EP0449770B1

Bacterial vectors

Abstract

This record has no abstract on file.

EP0449770B1, drawing sheet 1 — Sheet 1 of 11

Term

Term ended

Expired 13 March 2011, 15.5 years ago.

34 claims: 3 independent, 31 dependent

1
hybrid vector comprising a) the approximately 3.5 kbp EcoRI/SalI L. lactis insert of the plasmid pUCRS deposited under the accession number DSM 5803, or a functional fragment thereof retaining promoter, signal or structural functions, or b) a DNA sequence which hybridizes with said insert or with a functional fragment thereof, or such a hybridizing DNA sequence operatively linked to its natural promoter region or c) a degenerate sequence of the DNA sequence covered in a) which encodes a signal peptide,
18
A DNA molecule which a) is the approximately 3.5 kbp EcoRI/SalI L. lactis insert of the plasmid pUCRS deposited under the accession number DSM 5803, or a functional fragment thereof retaining promoter, signal or structural functions, or b) hybridizes with said insert or with a functional fragment thereof, or is such a hybridizing DNA sequence operatively linked to its natural promoter region, or c) is a degenerate sequence of the DNA sequence covered in a) which encodes a signal peptide.
33
The major secretory protein of L. lactis in pure form having the amino acid given in SEQ ID No. 1.

Independent claims3

226 paragraphs in 1 section, as filed

Field of the invention

The invention relates to the field of genetic engeneering and provides novel DNA molecules which comprise a DNA sequence coding for the most abundant protein in the supernatant of cultures of Lactococcuslactis, hereinafter named Major Secretion Product (MSP), for its signal peptide and/or the promoter of the MSP gene. The molecules may also comprise multifunctional origin of replication. The novel DNA molecules are used for the production of novel shuttle vectors for cloning of DNA in at least E. coli and Lactococcusspec. or novel vectors for the expression of homologous or heterologous genes and the production of secreted gene products in gram positive bacteria such as Lactococcusspec. and Bacillus spec.

Background of the invention

Although in genetic engineering techniques numerous procaryotic vector-host-systems for cloning of heterologous or homologous genes are already known, there is a continuous need for novel systems which may have advantages over the known systems.

Most recombinant work in DNA technology has been carried out with bacteria such as Escherichia coli or Bacillus subtilis. The lactic acid bacteria, however, are of much more industrial interest. For this reason, a number of efforts have been made to develop plasmid vectors for cloning and expression of homologous and heterologous genes in lactic acid bacteria, particularly in Lactobacillus spec. and Lactococcus spec. [see PCT WO 85/03945, Gasson and Anderson (1985), EP-A-0 316 677, Bates et al. (1989), Jos et al. (1985) and review articles of Chassy (1987) and of De Vos (1987)]. Lactococcusspec. was formerly named Streptococcusspec..

Lactococcal strains investigated so far harbour a characteristic plasmid complement consisting of multiple different plasmids. This property can be used to differentiate between various lactococcal strains (Davies et al., 1981). For genetic studies, plasmid free strains have been constructed by repeated curing in the course of plasmid function studies (De Vos, 1987).

For example, the plasmid complement of L. lactis 712, hereinafter also called L. lactis LL712, consists of 5 plasmids having the molecular weights of 1.8 Md, 2.5 Md, 5.2 Md, 9 Md and 33 Md which are named pSH71, pSH72, pSH73, pSH74 and pLP712, respectively (Gasson, 1983).

Based on plasmid pSH71 of L. lactis and on the related L.cremoris plasmid pWV01 (Otto et al., 1982) various cloning vectors have been constructed. The cloning vectors have been produced either by inserting genetic markers such as antibiotic resistance genes into the plasmids or by screening fragments of the plasmids for an origin of replication function, i.e. for the ability to sustain replication of selected DNA fragments. A cloning vector produced according to the latter method is pNZ12 which contains the 1.7 kbp ClaI restriction fragment of pSH71 comprising the origin of replication (Gasson and Anderson, 1985). The origin of replication of pSH71 is also functional in other gram-positive bacteria such as Bacillusspec. and in the gram-negative Escherichia coli.

On the basis of these plasmids cloning vectors useful for the introduction and expression of homologous or heterologous genes in lactic acid bacteria have been developed. The development of the cloning vectors resulted in transformed lactococcal strains with improved properties which are useful in food and feed industry, for example a bacteriophage resistant L. lactis strain (EP-A-0 316 677) or a L. lactis strain which produces bovine prochymosin (PCT WO 85/03945). The development of cloning vectors for the production of homologous or heterologous gene products is not only of interest because of the production of improved lactic acid bacteria cells but also for the production of recombinant proteins. One of the major problems with the production of heterologous proteins in microbial expression systems has been the purification of the product Purification of intracellular proteins is time-consuming and often results in poor yields. Purification can be considerably facilitated if the product is secreted from the host cell. To avoid the problems of purification of the products expressed in bacteria, vector-host systems for the production of recombinant proteins which are secreted into the supernatant can be useful.

Another advantage of secreted proteins can be that they can have a native and biologically active conformation, because then no refolding process is needed. Refolding is usually necessary if the polypeptide is intracellularly deposited.

Secretion of a protein usually requires a signal peptide at the amino terminus of the primary translation product which directs the protein into the secretory pathway. It is of advantage if the signal peptide is cleaved enzymatically from the protein during the translocation through the cell membrane. This, however, is not always the case.

Object of the invention and solutions

It is an object of the invention to provide novel hybrid vectors which can replicate in gram positive and gram negative bacteria and/or which allow expression of homologous or heterologous genes and secretion of stable protein products into the supernatant.

In particular, this object has been achieved by the present novel hybrid vectors comprising a novel DNA insert which comprises the promoter region, the DNA sequence coding for the signal peptide and the coding region of a hitherto unknown gene encoding a polypeptide which is the most abundant protein in the supernatant of L.lactis as judged after TCA precipitation of the supernatant, SDS-polyacrylamide electrophoresis of the precipitated proteins and staining of the gel with Coomassie brilliant blue and which is called herein Major Secretion Product (MSP).

Accordingly, another solution to an object of the invention is to provide novel hybrid vectors comprising the promoter region, the DNA sequence coding for the signal peptide and/or the coding region of the MSP gene or of a related gene and an origin of replication derived from the 2.5 Md or 5.2 Md plasmid of L. lactis LL712 or an origin of replication related thereto.

The invention concerns also functional fragments of the novel DNA inserts or the origins of replication per se. They are useful for the production of novel expression vectors for the secretion of homologous or heterologous proteins from lactic acid bacteria.

The invention further provides a method for the preparation of the novel DNA molecules and hybrid vectors, and a method for the production of secreted gene products by means of hosts transformed with a novel hybrid vector of the invention.

The invention provides also the MSP protein in pure form.

Detailed description of the invention

DNA molecules

The invention concerns a hybrid vector comprising <ul id="ul0001" list-style="none"><li>a) the approximately 3.5 kbp EcoRI/SalI L. lactis insert of the plasmid pUCRS deposited under the accession number DSM 5803, or a functional fragment thereof, or</li><li>b) a DNA sequence which hybridizes with said insert or with a functional fragment thereof, or comprises a promoter region which is naturally operatively linked to such a hybridizing DNA sequence, or</li><li>c) a degenerate sequence of a DNA sequence which is covered in a) and which encodes a signal peptide, or</li><li>d) a derivative of a DNA molecule covered in a), b) or c),</li></ul>

The vector may also comprise the origin of replication of (I) the 2.5 Md plasmid of L.lactis LL712 deposited under the accession number DSM 5804, or of (II) the 5.2 Md plasmid of L.lactis LL712, or of (III) a plasmid of the same incompatibility group as the 2.5 Md plasmid or the 5.2 Md plasmid of L.lactis LL712.

The approximately 3.5 kbp EcoRI/SalI L. lactis insert of the plasmid pUCRS comprises the promoter region of the MSP gene, the DNA sequence encoding the MSP signal peptide and the coding region for MSP. MSP with its signal peptide attached is hereinafter named pre-MSP. The EcoRI/SalI insert comprises the 1920 bp long DNA sequence with the SEQ ID No. 1 depicted in the sequence listing in such orientation that base position 1 is proximal to the EcoRI site.

The coding region for MSP extends from base position 492 up to base position 1793 of the sequence with SEQ ID No. 1.

The DNA sequence which encodes the 27 amino acid long MSP signal peptide, extends from the end of the promoter region defined hereinafter up to the beginning of the DNA sequence coding for the mature MSP, i.e. from base position 411 up to position 491 of the sequence with SEQ ID No. 1. The MSP signal peptide or a functional fragment thereof causes a protein to be excreted from a host cell, e.g. from a lactic acid bacterium, such as Lactococcus lactis, or from Bacillusspec., such as B.thuringiensis, if the N-terminus of the protein is covalently linked to the C-terminus of the signal peptide. Covalent linkage of the C-terminus of the MSP signal peptide and the N-terminus of a protein which is to be excreted from a host cell can be obtained by the production of a fusion gene in which the entire DNA sequence encoding the MSP signal peptide or part thereof encoding a functional fragment of the MSP signal peptide comprising the C-terminus is linked in proper reading frame to the 5' end of a structural gene encoding the desired protein. Such a fusion gene is, for example, the DNA molecule with SEQ No. 2 which comprises the DNA sequence encoding the MSP signal peptide and the hirudin structural gene.

The amino acid sequences of the MSP signal peptide and of MSP are also given in the sequence with SEQ ID No. 1.

The promoter region of the MSP gene is located upstream of the DNA sequence encoding the MSP signal peptide and comprises up to about 1500, preferably up to about 100 to 1000 nucleotides. In the approximately 3.5 kbp long EcoRI/SalI L.lactis insert of the plasmid pUCRS, the promoter region extends from the EcoRI cut end of the insert located upstream of the base corresponding to position 1 of the DNA sequence with SEQ ID No. 1 up to base position 411. More particular, the promoter region extends from the first HindIII restriction site located upstream of the base corresponding to position 1 up to base position 411. The promoter region can bind RNA polymerase as well as regulatory proteins and can control the expression of a structural gene operatively linked therewith.

The term functional fragment includes those DNA fragments retaining promoter, signal and/or structural functions.

Preferred functional fragments are those containig the MSP promoter region, the DNA sequence encoding the MSP signal peptide or the promoter region and the DNA sequence encoding the MSP signal peptide. Fragments according to the invention are also composed of smaller fragments retaining the MSP promoter activity and/or coding for a peptide with signal peptide activity.

Fragments with promoter function, for example, are those starting with the first base at the EcoRI cut end of the approximately 3.5 kbp EcoRI/SalI L. lactis insert or, in particular, those starting at the HindIII site and extending up to a base corresponding to about position 410 of the sequence with SEQ ID No. 1. Other fragments with promoter function are selected from the group of fragments starting with any one of the bases between the EcoRI and the HindIII site and ending with a base in about position 410 of the sequence with SEQ ID No. 1. Shorter fragments of the promoter region also retain promoter activity.

A DNA fragment which encodes a signal peptide extends, for example, from about base 411 up to base 491 of the sequence with SEQ ID No. 1. It can be extended at the 5' end with a fragment of the promoter region which does not retain promoter activity.

A fragment retaining MSP promoter function and encoding a signal peptide extends from the EcoRI cut end of the insert located upstream of the base corresponding to position 1 of the DNA sequence with SEQ ID No. No. 1 down to base position 491. Another fragment retaining MSP promoter function and encoding a signal peptide extends from the first HindIII restriction site located upstream of the base corresponding to position 1 of DNA sequence with SEQ ID No. 1 up to about the base corresponding to position 491. Other fragments retaining MSP promoter function and encoding a signal peptide are selected from the group of fragments starting with anyone of the bases between the said EcoRI and HindIII sites and ending with a base in about position 491 of the sequence with SEQ ID No. 1.

The fragments may contain linkers which provide for succesful linkage to other DNA molecules. Suitable linkers to above fragments have a DNA sequence which fits into the restriction site of the DNA to which the fragment is to be linked. They may also contain a predetermined restriction site.

A DNA molecule which hybridizes with said insert is hybridizing under conventional conditions. A conventional hybridization procedure is described e.g. by Benton and Davis (1977). Such a hybridizing DNA molecule comprises, for example, as hybridizing DNA sequence a variant of the structural gene encoding MSP. Such a hybridizing variant is hereinafter also referred to as a structural gene related to the MSP gene. The protein encoded by such a related gene is referred to as a protein related to MSP. Accordingly, a promoter region which is naturally operatively linked to a DNA sequence hybridizing with said insert is a promoter region of a gene related to the MSP gene.

A structural gene related to the MSP gene is, for example, a naturally occuring variant derived from another bacterium than L. lactis, particularly from another lactic acid bacterium, for example from Lactobacillusspec. or Lactococcusspec., e.g. L.cremoris or L.thermophilus. It is also a naturally occuring variant which is encoded by an isogene on the chromosome of or on a plasmid naturally occuring in L.lactis.

A DNA molecule comprising a gene related to the MSP gene can be isolated according to conventional methods.

DNA molecules of the invention are also such having degenerated DNA sequences. They are degenerated within the meaning of the genetic code in that an unlimited number of nucleotides are replaced by other nucleotides without changing the amino acid sequence for which they code. Molecules having such degenerate DNA sequences may be useful because of their different restriction sites or because of a preferred codon usage in a particular host. Preferred are degenerated sequences of the DNA sequence coding for the MSP signal peptide or of a variant thereof.

The term derivative when used in connection with a DNA molecule covered in a), b) or c) includes fragments, mutants or larger derivatives of such a DNA molecule. The term derivative includes also larger derivatives of fragments or mutants of such a DNA molecule. Preferred are fragments retaining promoter, signal or structural functions. Examples of such fragments are given above.

A mutant of a DNA molecule described in a), b) or c) is, for example, a DNA molecule having a deletion, insertion, inversion, or point mutation which may occur naturally or may be artificially introduced into the DNA molecule in vivo or in vitro according to conventional methods. A mutant can show an altered restriction pattern.

Larger derivatives of a DNA molecule covered in a), b) or c) are, for example, excisable from the L. lactis genome. They can be found in a genomic library of L.lactis LM0230 obtained by fragmentation of the nucleic acids, treatment of the fragments with a suitable restriction enzyme, e.g. Sau3AI, EcoRI, BamHI or HindIII, ligating into a suitable vector, e.g. the lambda phage λEMBL3 or the plasmid pBR322, cloning, e.g. in E. coli, and excising again, with the same or another suitable restriction enzyme.

Larger derivatives of a DNA molecule covered in a), b) or c) are also recombinant DNA molecules with flanking sequences, for example such comprising linkers which provide suitable restriction sites or which put regulatory sequences, such as a promoter, a DNA sequence encoding a signal peptide or a terminator, and a structural gene into the correct distance or reading frame, or such comprising sequences derived from a vector, e.g. from a phage or plasmid used in the construction of a hybrid vector. The flanking sequences comprised in the larger derivatives may give rise to fusion genes.

An origin of replication derived from the L. lactis LL712 2.5 Md or 5.2 Md plasmid or from a plasmid of the same incompatibility group as the 2.5 Md plasmid or the 5.2 Md plasmid can cause a plasmid to replicate in acid bacteria, for example in lactococci, e.g. in L.lactisL.lactisdiacetylactis or L.cremoris, and in E.coli or also in gram negative bacteria other than E. coli or in gram positive bacteria other than lactic acid bacteria, e.g. in Bacillusspec. such as B. thuringiensis.

An origin of replication which is derived from the L. lactis LL712 2.5 Md or 5.2 Md plasmid or from a plasmid of the same incompatibility group as the 2.5 Md plasmid or the 5.2 Md plasmid is, for example, comprised in the whole respective linearized plasmid obtainable after treatment with a suitable restriction endonuclease. Accordingly, a hybrid vector of the invention is also such comprising the whole DNA sequence of the 2.5 Md or the 5.2 Md plasmid or of a plasmid of the same incompatibility group as the 2.5 Md or 5.2 Md plasmid. Both plasmids can be isolated, for example, from the strain L.lactis LL712 which is deposited with the DSM as DSM 5804.

A DNA sequence which functions as an origin of replication is also a fragment of any of said plasmids retaining origin of replication function. Such a DNA fragment can be obtained, for example, by isolating it after the fragmentation of the respective plasmid or of a fragment thereof, for example, with physical forces, e.g. sharing forces, or with chemical cleavage reactions, or with enzymes cleaving DNA such as nucleases, e.g. the exonucleases Bal31, S1 or exonuclease III, or endonucleases, e.g. restriction endonucleases. In particular, such a DNA fragment is a restriction fragment obtained after treatment of the respective plasmid or fragment therof with one or with two different restriction endonucleases which are in the case of the 2.5 Md plasmid, for example, NdeI, SphI and/or EcoRV and in the case of the 5.2 Md plasmid, for example, Sau3A, NdeI, HindIII, AccI and/or EcoRI.

The origin of replication of the 2.5 Md plasmid is located, for example, on the approximately 1.8 kbp long NdeI/SphI fragment, the approximately 3.1 kbp long EcoRV/SphI fragment obtainable after partial EcoRV digestion of the approximately 3.5 kbp long SphI fragment, the approximately 3.5 kbp long SphI fragment, the approximately 1.2 kbp long NdeI/EcoRV fragment, the approximately 2.5 kbp long EcoRV fragment, or the approximately 3.5 kbp long EcoRV/SphI fragment obtainable after partial EcoRV digestion of the approximately 3.5 kbp long SphI fragment. The approximately 3.5 kbp long SphI and the other fragments mentioned hereinbefore are also comprised in pSC12. The production of pSC12 is described in Example 1.2. A restriction map is given in Figure 2.

The origin of replication of the 5.2 Md plasmid is located, for example, on the approximately 1.0 kbp long NdeI/HindIII fragment, the approximately 2.2 kbp long NdeI/AccI fragment, the approximately 2.7 kbp long NdeI/EcoRI fragment, the approximately 3.1 kbp long NdeVSau3A fragment, the approximately 2.0 kbp long Sau3A/HindIII fragment, the approximately 3.2 kbp long Sau3A/AccI fragment, the approximately 3.7 kbp long Sau3A/EcoRI fragment, or the approximately 4.1 kbp long Sau3A fragment. The fragments with Sau3A cut ends are obtainable after partial Sau3A digestion of the 5.2 Md plasmid, of pSC18 or of suitable restriction fragments of these plasmids. The production of pSC18 is described in Example 1.3. A restriction map is given in Figure 3.

Plasmids of the same incompatibility group cannot stably coexist in the same cell. They carry related origins of replication.

A recombinant DNA molecule does, for example, contain linkers and/or sequences derived from a vector such as from a phage or plasmid, and optionally a marker gene such as a resistance marker gene, e.g. an erythromycin, ampicillin, or tetracyclin resistance marker gene or the like. A recombinant DNA molecule comprising an origin of replication of the present invention is, for example, a vector plasmid for lactic acid bacteria, preferentially a shuttle vector which can replicate at least in lactic acid bacteria and E.coli and wherein a lactococcal derived origin of the invention is the only origin of replication.

A preferred shuttle vector is pSC12, pSC12ΔP, pSC12ΔN, pSC12ΔNP, pSC18, pSC18ΔP, or pSC18ΔN. A preferred shuttle vector for the expression of a foreign gene in lactic acid bacteria is pSC12HIR, pSC12HIR-1 or pSC12HIRTerm. The preferred shuttle vectors are described in the Examples.

A DNA sequence which functions as an origin of replication in at least lactic acid bacteria and E. coli which is intended to be covered above under e) is also an inversion, insertion, deletion or point mutant of a DNA sequence defined hereinbefore which retains the function of an origin of replication. Such mutants have an altered nucleotide sequence in the DNA sequences flanking the real origin of replication, for example point mutations which cause an altered pattern of restriction sites or deletion mutants such as the L. lactis inserts of the plasmids pSC12ΔP, pSC12ΔN, pSC12ΔNP, pSC18ΔNP, or pSC18ΔN (see Table 1). The real origin of replication mentioned hereinbefore is intended to be the smallest DNA fragment which functions as an origin of replication.

The hybrid vectors of the invention are usable for cloning in hosts such as fungi or in particular bacteria. They can be derived from any vector useful in the art of genetic engineering, such as from phages, cosmids or plasmids. They are, for example, derivatives of phage λ, e.g. NM 989 or EMBL 3, of phage M13, e.g. M13mp18 or M13mp19, of bacterial plasmids, e.g. pBR 322, pUC18 or pUC19 or plasmids of lactic acid bacteria, or of yeast plasmids, e.g. yeast 2µ plasmid, or also of defective phages or defective plasmids in the presence of a helper phage or a helper plasmid allowing replication of said defective phages or plasmids.

A hybrid vector of the invention comprises a nucleotide sequence of a DNA molecule covered in a), b), c) or d). It comprises in particular the approximately 3.5 kbp EcoRI/SalI L. lactis insert, a fragment thereof coding for the MSP signal peptide and/or the promoter of the MSP gene. Depending on the type of the DNA molecule of the invention inserted in the hybrid vector, the vector may comprise a hybrid expression control sequence. Such a hybrid expression control sequence partially consists of a DNA molecule covered in a), b) or c) and partially of a DNA molecule different from a DNA molecule covered in a), b) or c). For example, the hybrid vector can comprise the MSP promoter in combination with a DNA sequence coding for a non-MSP signal peptide a DNA fragment coding for the MSP signal peptide in combination with another than the MSP promoter. Additionally, a hybrid vector of the invention optionally comprises a transcriptional terminator region, e.g. the trpA transcription terminator of E. coli.

Hybrid vectors comprising a DNA sequence coding for a signal peptide of the present invention, i.e. derived from the gene for pre-MSP or a related gene, are useful for the production of secreted gene products in lactic acid bacteria, particularly in Lactococcus spec., especially in L. lactis or L.cremoris or in other bacteria, for example a Bacillus spec. such as B. thuringiensis. Such a hybrid vector comprises a promoter which functions in the desired bacterium, for example the MSP promoter for the expression in lactic acid bacteria, and optionally a transcription terminator region which functions in the desired bacterium. The use of a terminator can increase the yield of the recombinant gene product if it is operatively linked with the respective homologous or heterologous structural gene. For example, if the trpA terminator of E.coli is operatively linked with a hirudin gene (EP-168 342) which is expressed under the control of the MSP promoter and a DNA molecule encoding the MSP signal peptide in lactic acid bacteria, the production of desulfatohirudin (vgl. EP-142 860) is markedly increased.

A hybrid vector of the invention also comprises an origin of replication which functions in the desired host, e.g. in lactic acid bacteria and/or Bacillusspec. and/or E.coli.

A DNA sequence bearing an origin of replication which functions in at least lactic acid bacteria is, for example, the 1.7 kbp ClaI fragment of the lactococcal plasmid pSH71 (Gasson and Anderson, 1985) or one of the DNA fragments defined hereinbefore which comprise the origin of replication of the 2.5 Md plasmid or of the 5.2 Md plasmid of L. lactis LL712.

A hybrid vector of the present invention may contain a selectable marker. The choice of the marker depends on the host which is to be transformed, selected and cloned. Any marker gene can be used which facilitates the selection of transformants due to the phenotypic expression of the marker. Suitable markers are particularly those expressing antibiotic resistance, e.g. against erythromycin, tetracycline or ampicillin, or in the case of auxotrophic mutants, genes which complement host lesions, e.g. genes of the lactose metabolizing pathway.

Preferred embodiments of the present invention are hybrid vectors comprising a homologous or heterologous structural gene, for example the MSP or especially a heterologous structural gene defined hereinafter, which is operatively linked in a proper reading frame to the DNA sequence encoding the MSP signal peptide and which can be expressed under the control of the MSP promoter or a heterologous promoter.

Structural genes may originate from viruses, procaryotic cells or eucaryotic cells and may be derived from genomic DNA or from cDNA prepared via the mRNA route, or may be synthesized chemically. They may code for a wide variety of useful polypeptides, including glycosylated polypeptides, in particular of higher eukaryotic, especially mammalian, such as animal or especially human origin, such as enzymes which can be used, for example, for the production of nutrients and for performing enzymatic reactions in chemistry, or polypeptides which are useful and valuable for the treatment of human or animal diseases or for the prevention thereof, for example hormones, polypeptides with immunomodulatory, anti-viral and anti-tumor properties, antibodies, viral antigens, vaccines, clotting factors, foodstuffs, and the like.

Examples of such heterologous structural genes are e.g. those coding for hormones such as secretin, thymosin, relaxin, calcitonin, luteinizing hormone, parathyroid hormone, adrenocorticotropin, melanocyte-stimulating hormone, β-lipotropin, urogastrone or insulin, growth factors, such as epidermal growth factor, insulin-like growth factor (IGF), e.g. IGF-I and IGF-II, mast cell growth factor, nerve growth factor, glia derived nerve cell growth factor, or transforming growth factor (TGF), such as TGFβ, growth hormones, such as human or bovine growth hormones, interleukin, such as interleukin-1 or -2, human macrophage migration inhibitory factor (MIF), interferons, such as human α-interferon, for example interferon-αA, αB, αD or αF, β-interferon, γ-interferon or a hybrid interferon, for example an αA-αD- or an αB-αD-hybrid interferon, especially the hybrid interferon BDBB, proteinase inhibitors such as α1-antitrypsin, SLPI and the like, hepatitis virus antigens, such as hepatitis B virus surface or core antigen or hepatitis A virus antigen, or hepatitis nonA-nonB antigen, plasminogen activators, such as tissue plasminogen activator or urokinase, tumour necrosis factor, somatostatin, renin, β-endorphin, immunoglobulins, such as the light and/or heavy chains of immunoglobulin D, E or G, or human-mouse hybrid immunoglobulins, immunoglobulin binding factors, such as immunoglobulin E binding factor, e.g. sCD23, calcitonin, human calcitonin-related peptide, blood clotting factors, such as factor IX or VIIIc, erythropoietin, eglin, such as eglin C, hirudin, desulfatohirudin, such as desulfatohirudin variant HV1, HV2, HV3, [Leu1,Thr2]-HV1, [Lys47]-HV2 or PA, human superoxide dismutase, viral thymidin kinase, β-lactamase, glucose isomerase. A preferred gene is this coding for desulfatohirudin, e.g. variant HV1. In the hybrid vectors of the present invention, the present promoter and/or the DNA molecule encoding the MSP signal peptide is operably linked to the polypeptide coding region so as to ensure effective expression of the polypeptide.

Preferred hybrid vectors are pUCRS, M13 mp18RS, M13 mp19H, pVAHIR, pVAHIR-1, pSC12HIR, pSC12HIR-1, and in particular pSC12HIRTerm. The hybrid vectors are described hereinafter in the Examples.

The invention also concerns a DNA molecule which <ul id="ul0002" list-style="none"><li>a) is the approximately 3.5 kbp EcoRI/SalI L. lactis insert of the plasmid pUCRS deposited under the accession number DSM 5803, or a functional fragment thereof, or</li><li>b) hybridizes with said insert or with a functional fragment thereof, or comprises a promoter region which is naturally operatively linked to such a hybridizing DNA sequence, or</li><li>c) is a degenerate sequence of a DNA sequence which is covered in a) and which encodes a signal peptide, or</li><li>d) is a derivative of a DNA molecule covered in a), b) or c), per se.</li></ul>

The molecule may also comprise the origin of replication of (I) the 2.5 Md plasmid of L.lactis LL712 deposited under the accession number DSM 5804, or of (II) the 5.2 Md plasmid of L.lactis LL712, or of (III) a plasmid of the same incompatibility group as the 2.5 Md plasmid or the 5.2 Md plasmid of L.lactis LL712.

These DNA molecules are useful for the preparation of the hybrid vectors of the invention or for screening DNA gene libraries or mRNA for further similar DNAs or mRNAs.

Process for the Preparation of the DNA Molecules and of MSP

A further object of the invention is a process for the preparation of a DNA molecule of the invention, i.e. of a hybrid vector or a DNA molecule defined hereinbefore, comprising <ul id="ul0003" list-style="none" compact="compact"><li>A) culturing a host which comprises a DNA molecule of the invention and isolating a DNA molecule of the invention from such a cultured host, or</li><li>B) preparing a DNA molecule of the invention by an invitro synthesis.</li></ul>

The culturing of the hosts is carried out in a conventional nutrient medium which may be supplemented with or deprived of chemical compounds allowing negative or positive selection of the transformants, i.e. such hosts containing the desired DNA molecule together with a selection marker, from the non-transformants, i.e. such hosts lacking the desired DNA molecule.

Any suitable transformable hosts useful in the art may be used, for example suitable bacteria, such as gram-negative bacteria, e.g. E. coli, or gram-positive bacteria, e.g. Bacillus spec. or lactic acid bacteria, such as Lactobacillusspec., or especially Lactococcusspec., e.g. L.lactisL.lactisdiacetylactis or L. cremoris.

Bacteria are transformed by a conventional method and the transformants are identified in conventional manner, e.g. by their resistance, for example against tetracycline.

In particular the described hybrid vectors are propagated in suitable E. coli host strains, such as TG1, HB101 (Maniatis et al (1982)), JM109, MH1 and the like, or in suitable Bacillus strains, or in suitable lactic acid bacteria, e.g. Lactococcus strains such as L.lactis 0230, L.lactis diacetylactis,L. cremoris and the like. The hosts are transformed and selected by methods conventional in the art. The propagated plasmid DNA is isolated from the bacteria by conventional methods, for example as described by Birnboim & Doly (1979).

A DNA molecule of the invention can also be prepared by an in vitro synthesis according to conventional methods. The in vitro synthesis is especially applicable for the preparation of smaller fragments, e.g. of the DNA sequences of the MSP gene or of a related gene coding for the promoter or in particular for the signal peptide.

A DNA molecule of the invention can be obtained from a lactic acid bacterium containing such a DNA molecule, in particular from a genomic library thereof or also via mRNA.

In the following the preparation of a DNA molecule which is the approximately 3.5 kbp EcoRI/SalI L. lactis insert of plasmid pUCRS is described in more detail.

As starting material a genomic library of Lactococcus spec., e.g. L.lactis 0230, can be used which is prepared according to conventional methods, e.g. by partial digestion of genomic DNA of a L. lactis strain, e.g. LM 0230, C2 or LL712, with a restriction enzyme, e.g. Sau3A or MboI, and cloning the high molecular weight DNA fragments in a suitable vector, e.g. the E. coli plasmid pUN121 or a lambda phage, e.g. λEMBL3.

Any other strain of lactic acid bacteria producing MSP may also serve as source for the genomic library and likewise other suitable vectors may be used as recipient for the fragments.

In order to successfully screen the genomic library for DNA sequences of the present invention a DNA probe is necessary which hybridizes with such a DNA sequence, e.g. with the MSP structural gene. This can be a synthetic DNA probe if the sequence of e.g. the MSP gene or part thereof is known. As neither the MSP protein nor the MSP gene sequence or part thereof was known prior to the invention, the problems of purification of MSP, of the determination of the N-terminus sequence of MSP and of the preparation of hybridizing DNA probes were solved first.

MSP is the most abundant gene product in the supernatant of cultures of Lactococcus spec., e.g L.lactis as judged after TCA precipitation of the supernatant, SDS-polyacrylamide gel electrophoresis of the precipitated proteins and staining of the gel with Coomassie brilliant blue.

For the purification of MSP any source containing it may be used, for example, the supernatant of a culture of lactic acid bacteria, e.g. Lactococcusspec such as L. lactis. MSP can be purified from a SDS polyacrylamide gel by cutting out the gel piece comprising the major protein band and by eluting MSP.

Further purification methods such as precipitation with acid, e.g. trichloroacetic acid, salting out, desalting, reprecipitation in form of a different salt, dialysis chromatography, e.g. affinity chromatography, ion exchange chromatography, gel permeation chromatography, electrophoresis, e.g. with SDS-polyacrylamide gel, isoelectric focusing, electroelution, and the like, or any combination thereof can also be applied in order to obtain pure MSP.

In the present invention, a MSP with an apparent molecular weight of about 56 kD was isolated in pure form by precipitating the proteins from the supernatant of L.lactis LM0230 with trichloroacetic acid, by a subsequent electrophoresis of the precipitated protein on a SDS polyacrylamide gel, cutting out the region with the major protein band and electroeluting MSP from the gel. This method is also suitable for the isolation of proteins related to MSP having another apparent molecular weight. The amino acid sequence of MSP was partly determined by sequencing and partly deduced from the DNA sequence. It is shown in the sequence listing under SEQ ID No. 1 and extends from position 28 up to 466 of the amino acid sequence depicted.

The pure MSP protein is also a subject of the invention.

The sequencing of the N-terminus of MSP was performed in conventional manner and revealed the following amino acid sequence: X-X-Asn-Ser-Asp-Ile-Ala-Lys-Gln-Asp-Ala-Thr-Ile-Ser-X-Ala-Gln-Ser-Ala-Lys-Ala-Gln-Ala-Gln-Ala-Gln-Val-Asp. The first two amino acids and the amino acid in position 15 which are indicated with "X" have not been determined.

Based on the sequence of the amino acids in position 5 to 13 the following oligonucleotide mixture was synthesized: GAN1 ATN2 GCI AAN3 CAN3 GAN1 GCI AC. In this nucleotide sequence, N1 is T or C; N2 is T, C or A; N3 is A or G. A represents a nucleotide with the base Adenine, T with Thymine, C with Cytidine, G with Guanosine and I with Inosine. The oligonucleotide mixture was radioactively marked in conventional manner and used to screen a genomic library of Lactococcus spec., particularly L. lactis LM0230.

DNA probes containing sequences encoding MSP amino acid sequences and having at least about 14 bp can be used for screening for nucleic acids comprising related MSP genes or part thereof, which includes also the screening for pre-MSP.

For screening purposes the DNA probes are radioactively labelled at their 5' end by methods known in the art using γ32P-ATP and T4 kinase. Host microorganisms or phages carrying nucleic acids of the present invention as an insert, are identified by hybridization with the labelled DNA probe on filter replicas of the gene library.

The hybridization conditions used are conventional and may be more or less stringent, e.g. simply by choosing different temperatures.

The hybridizing part of a DNA clone of the library which hybridizes with the oligo-nucleotide mixture was partly sequenced according to conventional methods. The determined sequence comprises nearly the entire functional MSP gene. The sequence is depicted in the sequence listing under SEQ ID No. 1.

The 3.5 kbp EcoRI/SalI L. lactis insert can be isolated from positive clones of a genomic library by digestion with EcoRI and SalI and subsequent purification of the insert according to conventional methods, e.g. using agarose gel electrophoresis. Fragments can be prepared according to conventional methods and can be ligated into a suitable vector, e.g. into M13mp18 to generate M13mp18RS or into pUC18 to generate pUCRS.

Likewise, any other L. lactis insert comprising MSP sequences, for example larger derivatives, variants or fragments of the 3.5 kbp EcoRI/SalI insert, or DNA molecules which hybridize with the 3.5 kbp EcoRI/SalI insert or comprise a promoter region which is naturally linked to such a hybridizing DNA molecule can be isolated from a genomic library of lactic acid bacteria.

Fragments of the DNA molecule according to a) to c) may be obtained in conventional manner, e.g. by isolation of the fragments after digestion of the insert with suitable exo- or endonucleases, e.g. with exonuclease III, Bal31 or S1 or restriction endonucleases, such as Sau3A, HindIII, and the like. Fragments may also be obtained by in vitro DNA synthesis according to conventional methods.

Mutants of the DNA molecule covered in either of a) to c) hereinbefore, for example inversion, deletion, insertion or point mutants, can be prepared according to conventional methods, for example invivo or in vitro by site-directed mutagenesis (see review article of Zoller and Smith 1983, Botstein and Shortle, 1985, or Norris et al., 1983) using mutagenic oligonucleotide primers or by deleting DNA fragments between two restriction sites by cutting with suitable restriction enzymes and religating the DNA, optionally in diluted solution.

In the following the preparation of a recombinant DNA molecule according to the invention comprising a DNA sequence which functions as an origin of replication is described in more detail.

Plasmids are isolated from L. lactis LL712 according to conventional methods, e.g. as described by Birnboim and Doly (1979) with the modifications described in the examples, and are separated in conventional manner, e.g. using chromatography techniques, for example agarose gel chromatography as described in Maniatis et al. (1982). The 2.5 Md plasmid and the 5.2 Md plasmid are isolated and fragmented according to conventional methods.

The fragment mixtures thus obtained are ligated with a suitable vector according to conventional methods, e.g. as described in Maniatis et al. (1982) and these ligation mixtures are used to transform in conventional manner a suitable intermediate host strain.

A suitable vector carries a marker gene for selection in lactic acid bacteria, e.g. a resistance marker gene, for example the erythromycin resistance gene, and an origin of replication which does not function in lactic acid bacteria but in a bacterium suitable as an intermediate host for cloning the fragments of the L. lactis plasmids. A suitable vector comprises also a marker gene for selection in the intermediate host which may be identical with the marker gene which functions in the lactic acid bacterium. A suitable intermediate host is, for example, an E. coli strain, e.g. E.coli TG1, and a suitable cloning vector then is an E. coli vector, for example a pUC18 derivative carrying an erythromycin resistance gene, such as pUC383, the construction of which is described hereinafter in the Examples.

The intermediate host cells which are transformed with a vector comprising fragments of the L. lactis 2.5 Md or 5.2 Md plasmid optionally may be selected in conventional manner which depends on the type of intermediate host cell and the vector used. Selection markers may be, for example, resistance markers or genes encoding a screenable marker enzyme, e.g. the product of the E. coli lacZ gene, β-galactosidase, provided that the host is an E. coli strain defective in the genomic lacZ gene. The selection marker genes may be disturbed by the insertion of a DNA fragment. If the marker is lacZ, the host cells which carry a vector with a fragment inserted into the lacZ gene are no more able to convert X-Gal into a blue dye and, as a consequence, positive clones on X-Gal comprising agar plates remain white after induction of the expression of the lacZ gene with IPTG.

Vectors carrying an insert derived from the L. lactis 2.5 Md or 5.2 Md plasmid are tested for their ability to replicate in a plasmid free L. lactis strain, e.g. in L. lactis 0230. For this purpose the plasmids are isolated from the intermediate host and transformed into L. lactis 0230 cells according to conventional methods, e.g. as described in Powell et al. (1988). Replicating vectors comprise a DNA insert derived from the L. lactis 5.2 Md or 2.5 Md plasmid which functions as an origin of replication. A DNA molecule comprising this function may be isolated from the replicting vectors, fragmentated, mutated and the like and can be used to construct recombinant DNA molecules according to the invention, e.g. cloning or expression vectors replicating in lactic acid bacteria. The L. lactis 2.5 Md or 5.2 Md plasmid derived origins of replication are also functional in bacteria other than lactic acid bacteria, e.g. in Bacillus spec. or E. coli. Therefore, vectors comprising such DNA fragments can be used as shuttle vectors.

Fragments of the 2.5 Md or 5.2 Md plasmid carrying an origin of replication can also be identified and isolated according to the method described hereinbefore from a fragment mixture which was obtained by fragmentation of the whole plasmid pool of L. lactis LL712. Which of the cloned fragments carrying an origin of replication is derived from the 2.5 Md or 5.2 Md plasmid may be determined by hybridizing the cloned fragments with the plasmids of the plasmid pool derived from L.lactis LL712 which were separated on an agarose gel or by comparison of the restriction pattern of the cloned fragments and of the plasmids of the pool.

Recombinant DNA molecules according to the invention which comprise the whole DNA sequence of the L. lactis 2.5 Md or 5.2 Md plasmid or a plasmid of the same imcompatibility group as the 2.5 Md or 5.2 Md plasmid may be obtained, for example, by cutting the respective plasmid with a suitable restriction endonuclease and ligating it, for example, with a DNA fragment comprising a homologous or heterologous structural gene, a promoter region or vector sequences or with a linker fragment or the like. Suitable restriction endonucleases have only one recognition and cleavage site in the respective plasmid.

A recombinant DNA molecule comprising a sequence with origin of replication function derived from the 2.5 Md or the 5.2 Md plasmid can be obtained, for example, by a method comprising the preparation of the plasmid pool of L. lactis LL712 according to conventional methods, the identification of the 2.5 Md or 5.2 Md plasmid, e.g. by estimating the molecular weight in agarose gel electrophoresis, fragmentation of the respective plasmid, e.g. with suitable restriction enzymes, preparation of a fragment comprising the origin of replication function, e.g. one of the fragments mentioned hereinbefore, and ligating such a fragment or a mixture comprising such fragment with a DNA molecule comprising no origin of replication, e.g. a cloning vector depleted of its origin of replication, and selecting for DNA molecules replicating in at least L. lactis and E. coli.

Plasmids comprising an origin of replication of the same incompatibility group as the 2.5 Md or 5.2 Md plasmid can be identified because they cannot be maintained together with the 2.5 Md or 5.2 Md plasmid, respectively, within the same cell.

The invention concerns also the use of a DNA molecule or a recombinant DNA molecule of the invention for the preparation of hybrid vectors for the expression of a structural gene. Examples of such structural genes are given hereinbefore. The hybrid vectors can be prepared according to conventional methods using enzymes such as restriction enzymes, DNA polymerases, DNA ligases and the like.

Example 4 and 5 describe exemplary in more detail the preparation of hybrid vectors for the expression of a structural gene in lactic acid bacteria, particularly in L.lactis, or in Bacillus spec., particularly B. thuringiensis.

Transformed hosts and process for the preparation thereof

The invention concerns further a bacterial host transformed with a hybrid vector of the invention.

Transformed bacterial hosts according to the invention are suitable for the cloning, amplification and/or preparation of a hybrid vector comprising a DNA molecule defined in of a) to e). The hybrid vectors can replicate in such hosts and are not lost under selective pressure in the cell population during proliferation. The host which can be used depends on the origin of replication comprised in the hybrid vector. In the case that the hybrid vector comprises a DNA sequence with origin of replication function according to e), a suitable host is, for example, any strain of E.coli, Bacillusspec. or Lactococcusspec. which does not contain a plasmid with an origin of replication of the same incompatibility group.

In the case that the hybrid vector comprises a homologous or heterologous structural gene fused in a proper reading frame with the DNA sequence coding for the MSP signal peptide, a transformed host of the invention is such which is suitable for the production of a secreted homologous or heterologous protein, e.g. a strain of L. lactis.

An example for a transformed host according to the invention is an E. coli strain, e.g. TG1, C600 (Huynh et al (1985)) or HB101 transformed with pUCRS, pSC12, pSC12ΔP, pSC12ΔN, pSC12ΔNP, pSC18, pSC18ΔN, pSC18ΔP, pVAHIR, pVAHIR-1, pSC12HIR, pSC12HIR-1, or pSC12HIRTerm, or L.cremoris, or a plasmid-free L. lactis, e.g. L. lactis LM0230, or a Bacillus strain, e.g. B. thuringiensis, transformed with either of these plasmids. Preferred are E. coli TG1 transformed with pUCRS, L.lactis transformed with pUCRS, pSC12 HIRTerm, pSC12 or pSC18, and B. thuringiensis transformed with pVAHIR or pVAHIR-1.

The invention concerns also a method for the preparation of such transformants comprising treatment of a host under transforming conditions with a recombinant DNA molecule of the present invention, especially a hybrid vector of the invention, optionally together with a selection marker gene and selecting the transformants.

Process for the preparation of polypeptides

The invention concerns further a method for the preparation of a polypeptide, characterized in that a homologous or heterologous structural gene is fused in proper reading frame with the DNA sequence coding for the MSP signal peptide, that a suitable host such as a gram positive bacterial host, for example a lactococcal host, e.g. L.lactis LM0230, or Bacillusspec. e.g. B.thuringiensis, is transformed with a hybrid vector comprising such fused gene and that the polypeptide encoded by said gene is secreted from the host cells. When required, the polypeptide is isolated from the supernatant according to conventional methods. In a preferred embodiment of the invention a bacterial host is used which does not secrete a protease in its culture medium which degrades the expressed protein.

Such a preferred embodiment of the invention is, for example, the production of proteins, e.g. desulfatohirudin, which are secreted from L. lactis LM0230 cells transformed with a hybrid vector of the invention comprising a gene encoding such protein, e.g. pSC12HIR, pSC12HIR-1 or in particular pSC12HIRTerm. Concentrations of active desulfatohirudin can be obtained from supernatant of stationary phase cultures of L. lactis LM0230 cells transformed with pSC12HIR or pSC12HIR-1. In the supernatant of L. lactis LM0230 cells transformed with pSC12HIRTerm the yield in desulfatohirudin is increased. Surprisingly, the levels of heterologous gene products, e.g. desulfatohirudin, do not decrease after prolonged incubation, e.g. over night, of the stationary phase cultures indicating absence of proteolytic degradation of the heterologous gene products in the supernatant.

In another embodiment of the invention the cells may be collected from the nutrient medium either in the log phase or in the stationary phase of the culture, e.g. by centrifugation or filtration, and resuspended in a smaller volume, e.g. in about 1 % up to 20 % of the original culture volume, of fresh nutrient medium or suitable buffer solution. Incubation of the resuspended cells results in an increased yield of heterologous gene product which is secreted into the supernatant. For example, in a culture of L. lactis LM0230 transformed with pSC12HIR an increased yield in desulfatohirudin can be obtained in the supernatant if the cells of a stationary phase culture are collected by centrifugation, resuspended in about 1/10 vol. of fresh nutrient medium and incubated for about 30 min at about 30°C.

Another embodiment of the invention is the production of MSP which is secreted from a gram positive host cell transformed with a hybrid vector of the invention, e.g. pUCRS, according to the methods described above.

A further embodiment of the invention is the production of desulfatohirudin in B. thuringiensis, preferentially in strain HD1 cryB (DSM 4574), transformed with pVAHIR or pVAHIR-1.

Short description of the figures

Figure 1: Physical map of plasmid pUC838. The map positions are approximate and given in kbp. The pUC 18 mojety extends from map position 0 up to 2.7 and the pVA838 mojety carrying the erythromycin resistance gene extends from map position 2.7 up to 4.4. The restriction sites shown in brackets were destroyed during the preparation of the recombinant molecule. The meanings of the abbreviations are: ampr, ampicillin resistance gene; eryr, erythromycin resistance gene; oripuc, origin of replication derived from pUC 18.

Figure 2: Physical map of plasmid pSC12. The map positions are approximate and are given in kbp. pUC18 DNA extends from position 0 up to 2.7. The 1.7 kb erythromycin resistance carrying fragment of pVA838 extends from position 2.7 up to 4.4. The L.lactis 2.5 Md plasmid derived insert extends from position 4.4 up to 8.0/0. The restriction sites shown in brackets were destroyed during the preparation of the recombinant molecules. The meanings of the abbreviations are: PL1, pUC polylinker region from the EcoRI to the (Smal) site, PL2, pUC polylinker region from the (Smal) to the SphI site; ampr, ampicillin resistance gene; eryr, erythromycin resistance gene; oripuc, origin of replication derived from pUC; ori11, origin of replication derived from lactococcal 2.5 Md plasmid as conducted from deletion analysis.

Figure 3: Physical map of plasmid pSC18. The map positions are approximate and are given in kbp. pUC18 DNA extends from position 0 up to 2.7. The 1.7 kb erythromycin resistance carrying fragment of pVA838 extends from position 2.7 up to 4.4. The L.lactis 5.2 Md plasmid derived insert extends from position 4.4 up to 8.5/0. The restriction sites shown in brackets were destroyed during the preparation of the recombinant molecules. The meanings of the abbreviations are: PL1, pUC polylinker region from the EcoRI to the (SmaI) site; PL2, pUC polylinker region from the (BamHI) to the HindIII site; ampr, ampicillin resistance gene; eryr, erythromycin resistance gene; oripuc, origin of replication derived from pUC; ori12, origin of replication derived from lactococcal 5.2 Md plasmid as conducted from deletion analysis.

Abbreviations

<dl id="dl0001" compact="compact"><dt>dNTP</dt><dd>deoxy nucleotide trisphosphate</dd><dt>HPLC</dt><dd>high performance liquid chromatography</dd><dt>IPTG</dt><dd>isopropyl-β-D-thiogalactopyranoside</dd><dt>kbp</dt><dd>kilo base pairs</dd><dt>kD</dt><dd>kilo Daltons</dd><dt>LB</dt><dd>Luria broth nutrient medium (Gibco/BRL)</dd><dt>Md</dt><dd>Mega Daltons</dd><dt>sCD23</dt><dd>soluble CD23, i.e. 25 k IgE-binding factor</dd><dt>SLPI</dt><dd>Secretory Leucoproteinase Inhibitor</dd></dl>

The following examples serve to illustrate present invention, however, they should not be construed as a limitation thereof.

Material and Methods

Strains

L. lactis LM0230 is a plasmidfree derivative of L. lactis C2 (Efstathiou J.D. and L.L McKay, 1977). It is deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (see hereinafter).

L. lactis C2 (NCDO 2031), and L. lactis LL712 which latter serve as the source of the 2.5 Md and 5.2 Md plasmids, are closely related or identical (F.L. Davis et al., 1981). The former is obtainable, for example, from the National Collection of Diary Organisms, UK (NCDO 2031), the latter is deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (see hereinafter).

E. coli TG1 has the genotype K12, Δ(lac-pro), supE, thi, hsdD5/F'traD36, proA+B+, lacIq, lacZΔM15 and is obtainable from Amersham; it is described in the manual "Oligonucleotide-directed invitro mutagenesis system" of Amersham.

General Methods:

Transformation

E. coli is transformed according to the calcium chloride method as described in Maniatis et al. (1982).

Transformation of lactococcal strains is performed by electroporation using a Gene Pulser® apparatus from BioRad and following their protocol for L. lactis LM0230.

Plasmid Preparation

Plasmid DNA from E. coli is prepared according to the method of Birnboim and Doly (1979).

The same method is used for the isolation of plasmids from lactococci with the following modifications: <ul id="ul0004" list-style="none" compact="compact"><li>a) Overnight cultures are grown in M17-G medium (Terzaghi and Sadine, 1975) at 30°C, diluted 1:10 into fresh medium and grown for another two hours.</li><li>b) Incubation with lysozyme is performed also in the presence of mutanolysin (50 mg/l) for 10-20 min at 37°C.</li></ul>

A plasmid pool from L. lactis LL712 is prepared by the same method and the plasmid fraction is further purified by CsCl-ethidium bromide equilibrium centrifugation (Clewell and Helinski, 1969). The three smaller plasmids (1.8 Md, 2.5 Md, 5.2 Md; Gasson, 1983) are enriched in the purified plasmid fraction while the two larger plasmids (9 Md and 33 Md; Gasson, 1983) are present in smaller quantities.

Examples:

1. Construction of Shuttle Vectors for Lactic Acid Bacteria

and E. coli

1.1 Construction of pUC838

pVA838 (ATCC 37160; Macrina et al., 1982) is a lactococcal plasmid which carries the gene for constitutively expressed erythromycin resistance on a 1.7kbp AvaI/HindIII restriction fragment. About 10 µg of pVA838 DNA is digested with AvaI and HindIII. The ends of the DNA fragments are blunt ended with Kleenow enzyme in the presence of all four dNTPs. The fragments are separated on an agarose gel and the 1.7kbp AvaI/HindIII fragment is recovered from the gel by electroelution.

About 200 ng of said fragment and 100 ng of SmaI-cut pUC18 (Norrander et al.) are ligated in the presence of T4-ligase as suggested by Rusche et al. and the ligation mixture is used to transform E. coli TG1. White colonies are picked from LB agar plates containing X-Gal, IPTG and 100 mg/l of ampicillin. Correct clones are identified by isolating and analyzing their plasmid DNA, and by their ability to grow on LB agar plates containing erythromycin (100 mg/l). The resulting plasmid is designated pUC838. The restriction sites and characteristics of pUC838 are given in Figure 1.

1.2 Construction of pSC12

The plasmids in the pool isolated from L. lactis LL712 are separated on a preparative agarose gel. The second DNA band from bottom corresponds to the ccc form of the 2.5 Md plasmid. It is cut out and the plasmid is electroeluted. Digestion of this plasmid with SphI and agarose gel electrophoresis reveales a single 3.5 kbp fragment indicating the presence of a single or several closely spaced SphI cleavage sites in the 2.5 Md plasmid.

pUC838 is cut at its unique SphI site and treated with calf intestinal phosphatase. About 50 ng of phosphatase treated vector and 150 ng of the 3.5 kbp SphI fragment of the 2.5 Md plasmid is ligated with T4 ligase and the ligation mixture is used to transform competent E.coli TG1 cells. The transformation mixture is grown at 37°C in 1 ml of LB for 90 min and then transferred to 200 ml of LB containing 100 mg/l of erythromycin and grown at 37°C over night. Plasmid DNA is prepared from the cells of this culture and about 2 µg are used to transform L. lactis LM0230. Transformed L. lactis LM0230 cells are selected at 30°C on M-17G agar plates containing 5 mg/l of erythromycin.

Plasmid DNA is isolated from several clones of the transformed L. lactis LM0230 cells and subjected to restriction enzyme analysis. All transformants investigated contained a 8.0 kbp plasmid, pSC12, from which the 3.5 kbp SphI insert can be recovered. A physical map of pSC12 is given in Figure 2.

pSC12 is shuttled several times between L. lactis and E. coli without any obvious changes in restriction pattern.

1.3 Construction of pSC18

Plasmid pool DNA from L. lactis LL712 is partially restricted with Sau3AI by incubating 500 ng aliquots of DNA with different amounts of restriction enzyme. Samples containing partially cut DNA are identified on an agarose gel. This DNA is ligated to pUC838 vector which is previously cut at its unique BamHI site and treated with calf intestinal phosphatase.

Transformation of E. coli TG1 plasmid isolation and subsequent transformation of L. lactis LM0230 is done as described for pSC12. Again, restriction digests are performed on plasmid DNA isolated from several clones of L. lactis LM0230 transformants. They carry a 8.8 kbp plasmid, pSC18. Shuttling the plasmid several times between L. lactis and E. coli didn't result in any changes in restriction pattern indicating stability of the construct in both cell types. A physical map of pSC18 is given in Fig. 3.

2. Identification of Lactococcal origins of replication

2.1 Deletion Analysis of pSC12 and pSC18

The regions on plasmids pSC12 and pSC18 carrying the lactococcus derived origins of replication are defined by deletion analysis as follows:

DNA between appropriate restriction sites is deleted by cutting the plasmids with the corresponding restriction enzyme and recircularization with T4 ligase. The approximate location of the restriction enzyme cleavage sites in pSC12 and pSC18 are given in Figures 2 and 3, respectively, and in Table 1.

pSC12ΔP and pSC18ΔP are constructed by deleting the DNA between the two PvuII sites in the pUC18 part of the plasmids pSC12 and pSC18, respectively, thus removing the col El derived origins of replication.

In pSC12ΔN and pSC18ΔN the smaller NdeI-fragment of pSC12 or pSC18, respectively, is removed.

pSC12ΔR lacks an EcoRV fragment in the lactococcal DNA of pSC12.

In pSC12ΔNP DNA between the PvuII site in pUC and the NdeI site in the lactococcal part of pSC12 is removed. In this case the vector DNA is blunted with Kleenow enzyme before religation.

A further derivative of pSC18, pSC18ΔH, lacks DNA spanning the HindIII sites in the plasmid.

All the mutated plasmid DNAs are first isolated from E. coli TG1 transformants that are resistant to erythromycin. It is then tested whether they can be used to transform L. lactis LM0230 to erythromycin resistance. Failure to do so is assumed to indicate that the lactococcal origin of replication was removed or destroyed by the deletion. When erythromycin resistant transformants are obtained, the plasmid is reisolated and its structural integrity reconfirmed by restriction analysis.

For both the 2.5 Md and the 5.2 Md plasmid a region required for replication in streptococcus is identified. From the fact that the ΔP-derivatives could replicate in E. coli it is obvious that the cloned lactococcal origins can also function in the gram-negative E. coli bacteria.

Results of these experiments are given in Table 1. <tables id="tabl0001" num="0001"><table frame="all"><title>Table 1:</title><tgroup cols="5" colsep="1" rowsep="0"><colspec colnum="1" colname="col1" colwidth="31.50mm" /><colspec colnum="2" colname="col2" colwidth="31.50mm" /><colspec colnum="3" colname="col3" colwidth="31.50mm" /><colspec colnum="4" colname="col4" colwidth="31.50mm" /><colspec colnum="5" colname="col5" colwidth="31.50mm" /><thead valign="top"><row rowsep="1"><entry namest="col1" nameend="col5" align="left">Results of the deletion analysis of pSC12 and pSC18</entry></row><row rowsep="1"><entry namest="col1" nameend="col1" rowsep="0" align="left">Plasmid</entry><entry namest="col2" nameend="col2" rowsep="0" align="left">Deleted region1</entry><entry namest="col3" nameend="col3" rowsep="0" align="left">Deleted fragment2</entry><entry namest="col4" nameend="col5" align="center">Replication of the plasmid in</entry></row><row rowsep="1"><entry namest="col1" nameend="col1" /><entry namest="col2" nameend="col2" /><entry namest="col3" nameend="col3" /><entry namest="col4" nameend="col4" align="center">E. coli</entry><entry namest="col5" nameend="col5" align="center">L. lactis</entry></row></thead><tbody valign="top"><row><entry namest="col1" nameend="col1" align="left">pSC12</entry><entry namest="col2" nameend="col2" align="left">none</entry><entry namest="col3" nameend="col3" /><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">+</entry></row><row><entry namest="col1" nameend="col1" align="left">pSC12ΔP</entry><entry namest="col2" nameend="col2" align="left">PvuII (0.1), PvuII (2.5)</entry><entry namest="col3" nameend="col3" align="char" char=".">2.4</entry><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">+</entry></row><row><entry namest="col1" nameend="col1" align="left">pSC12ΔN</entry><entry namest="col2" nameend="col2" align="left">NdeI (0.2), NdeI (6.2)</entry><entry namest="col3" nameend="col3" align="char" char=".">2.0</entry><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">+</entry></row><row><entry namest="col1" nameend="col1" align="left">pSC12ΔR</entry><entry namest="col2" nameend="col2" align="left">EcoRV (5.0), EcoRV (7.5)</entry><entry namest="col3" nameend="col3" align="char" char=".">2.5</entry><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">-</entry></row><row><entry namest="col1" nameend="col1" align="left">pSC12ΔNP</entry><entry namest="col2" nameend="col2" align="left">NdeI (6.2), PvuII (2.5)</entry><entry namest="col3" nameend="col3" align="char" char=".">4.3</entry><entry namest="col4" nameend="col4" align="center">ND3</entry><entry namest="col5" nameend="col5" align="center">+</entry></row><row><entry namest="col1" nameend="col1" align="left">pSC18</entry><entry namest="col2" nameend="col2" align="left">none</entry><entry namest="col3" nameend="col3" /><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">+</entry></row><row><entry namest="col1" nameend="col1" align="left">pSC18ΔP</entry><entry namest="col2" nameend="col2" align="left">PvuII (0.1), PvuII (2.5)</entry><entry namest="col3" nameend="col3" align="char" char=".">2.4</entry><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">+</entry></row><row><entry namest="col1" nameend="col1" align="left">pSC18ΔN</entry><entry namest="col2" nameend="col2" align="left">NdeI (0.2), NdeI (7.5)</entry><entry namest="col3" nameend="col3" align="char" char=".">1.2</entry><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">+</entry></row><row rowsep="1"><entry namest="col1" nameend="col1" align="left">pSC18ΔH</entry><entry namest="col2" nameend="col2" align="left">HindIII (0), HindIII (6.5)</entry><entry namest="col3" nameend="col3" align="char" char=".">2.0</entry><entry namest="col4" nameend="col4" align="center">+</entry><entry namest="col5" nameend="col5" align="center">-</entry></row></tbody></tgroup><tgroup cols="5" colsep="0" rowsep="0"><colspec colnum="1" colname="col1" colwidth="31.50mm" /><colspec colnum="2" colname="col2" colwidth="31.50mm" /><colspec colnum="3" colname="col3" colwidth="31.50mm" /><colspec colnum="4" colname="col4" colwidth="31.50mm" /><colspec colnum="5" colname="col5" colwidth="31.50mm" /><tbody valign="top"><row><entry namest="col1" nameend="col5" align="justify">1The deletions extend between two restriction sites. The approximate positions of the restriction sites in pSC12 or pSC18 are given in the brackets as the distances from the HindIII site in the pUC838-derived mojety of the plasmids which is defined as position (0). They relate to the base positions given in Figures 2 and 3, respectively.</entry></row><row><entry namest="col1" nameend="col5" align="justify">2Approximate length of the deleted fragment in kbp.</entry></row><row><entry namest="col1" nameend="col5" align="justify">3Not determined.</entry></row></tbody></tgroup></table></tables>

2.2 Source of the lactococcal origins of replication

The origin of replication in pSC12 is derived from the 2.5 Md plasmid of L. lactis LL712 as is clear from the isolation protocol for the DNA fragment carrying the origin.

The restriction enzyme cleavage pattern indicates that the origin of replication in pSC18 stems from the 5.2 Md plasmid.

2.3 Replication of pSC12 and pSC18 in other Lactic Lactococci

Strains of L. lactis, L. lactis diacetylactis and L. cremoris are successfully transformed with both plasmids. The plasmids remain stably associated with the cell population under selective pressure.

3. Cloning of the Gene encoding the Major Secretion Product

(MSP) of L. Lactis LM 0230

3.1. Isolation of the Major Secreted Protein (MSP) of L.

Lactis LM 0230

1.5 l of an overnight culture of L. lactis LM 0230, grown at 30 °C in M-17G, is centrifuged in a Sorvall GS-3 rotor at 7000 rpm for 20 min at 4 °C. The supernatant is collected and proteins precipitated by adding an equal volume of ice cold 10 % trichloroacetic acid and incubating for 30 min at room temperature. The precipitate is collected by centrifugation in a Sorvall GS-3 rotor at 8 000 rpm and 4 °C for 30 min. The protein pellets are drained and redissolved in 3 ml of SDS-sample buffer (Laemmli, 1970). The sample is neutralized by adding a small amount of 4N NaOH and then dialyzed for 4 h against 4 l of 25 mM Tris-HCl pH 6.8, 0.02 % SDS. The sample recovered has a volume of about 4 ml. 3x conc. SDS-sample buffer is added to the sample in order to enlarge the volume to 6 ml.

Proteins are separated by running 2 ml aliquots on preparative 8 % SDS polyacrylamide gels (Laemmli, 1970) using a Protean cell from BioRad. Small strips are cut alongside from the gel, stained with Coomassie Brilliant Blue and destained in an aqueous solution containing 10 % of Methanol and 10 % of Acetic acid. They serve as markers to identify and cut out the major protein band with an apparent molecular weight of about 56 kD which is cut out of the gel.

The MSP protein is recovered from the gel by electroelution at 150 V for 2 h using a Biotrap apparatus and a buffer containing 20 mM ammonium acetate and 0.01 % SDS. The eluate is dialyzed for 48 h against two changes of 20 mM ammonium acetate, 0.005 % SDS. A sample of the dialyzed protein is run on a 8 % SDS-PAGE and a single sharp protein band is observed with an apparent molecular weight of about 56 kD.

3.2 Analysis of the amino-terminal Sequence of MSP

Amino-terminal sequencing of the isolated MSP protein is performed according to conventional methods using a gas phase sequenator (Applied Biosystems Inc., Model 470A) with HPLC quantitation of phenylthiohydantoin-derivatives of cleaved amino acid residues.

The 28 amino acid long sequence obtained is X-X-Asn-Ser-Asp-Ile-Ala-Lys-Gln-Asp-Ala-Thr-Ile-Ser-X-Ala-Gln-Ser-Ala-Lys-Ala-Gln-Ala-Gln-Ala-Gln-Val-Asp. The first two amino acids and the amino acid in position 15 which are indicated with "X" are not determined.

3.3 Synthesis of a mixed Oligonucleotide Probe

Based on the amino acid sequence Asp-Ile-Ala-Lys-Gln-Asp-Ala-Thr-Ile, which corresponds to the amino acids in position 5 to 13 in the N-terminus sequence of MSP, a mixed oligonucleotide is designed and constructed for the screening of a DNA library. Inosine is inserted at the two positions in the 23-mer where the degeneracy of the genetic code would require all 4 dNTPs in order to decrease the complexity of the probe. The nucleotide sequence of the oligonucleotide mixture is GAN1 ATN2 GCI AAN3 CAN3 GAN1 GCI AC. N1 is T or C; N2 is T, C or A; N3 is A or G. A represents a nucleotide with the base Adenine, T with Thymine, C with Cytidine, G with Guanosine and I with Inosine.

3.4. Construction of a Genomic Library of L. lactis LM 0230

Chromosomal DNA is isolated from L. lactis LM 0230 by a modification of the protocol used for plasmid isolation: after treatment with lysozyme and mutanolysin the cells are incubated for two hours at 56°C with proteinase K (100 mg/l) in 10 mM Tris-HCl pH 8, 20 mM EDTA, 0.5 % SDS. The DNA is extracted once with phenol/chloroform (1 vol:1 vol) and then purified by CsCl density gradient centrifugation.

Chromosomal DNA is partially digested with Sau3AI and size fractionated on sucrose gradients as described in Maniatis. Fragments between about 10-20 kbp are collected.

λ EMBL 3 DNA is cleaved with BamHI and EcoRI and purified by phenol extraction and ethanol precipitation. It is then ligated with the 10-20 kbp fragments in the presence of hexamminecobalt(III)chloride to favour the formation of concatamers. (Rusche J.R. et al., 1985). The ligation mixture is packaged in vitro using the Gigapack Plus® system from Stratagene.

Recombinants are selected by plating the library on E. coli Q 359 (Kahn J. et al., 1980). A total of about 600 000 recombinant phages is obtained.

3.5 Screening the Library for DNA sequences encoding MSP

About 15 000 recombinant λ EMBL 3 plaques are plated per petri dish (15 cm diameter) and transferred to PlaqueScreen® membranes (NEN). The filters are screened by hybridization with the mixed oligonucleotide probe described in Example 3.3 which was labeled with [γ32P]ATP using T4 Kinase according to standard procedures described in Maniatis et al. (1982).

Positive plaques are identified and subjected to a second round of screening at low plaque density.

DNA is prepared from positive phages, digested with restriction enzymes and subjected to Southern analysis as described in Maniatis. Southern blots are probed with the mixed oligonucleotide. It hybridizes to a 3.5 kbp EcoRI/SalI fragment in the L. lactis insert of a positive phage. Further probing showed a 2.1 kbp HindIII fragment within the 3.5 kbp EcoRI/SalI fragment to hybridize with the oligonucleotide mixture.

The approximate distances of different restriction sites from the EcoRI cut end of the 3.5 kbp EcoRI/SalI fragment are determined by agarose gel electrophoresis after digestion of the fragment with the respective enzymes and suitable enzyme mixtures. The EcoRI cut end is located about 0.5 kbp away from a HindIII site, about 2.6 kbp from a second HindIII site, and about 3.15 kbp from a third HindIII site.

3.6 Construction of pUCRS

DNA from a positive phage is digested with EcoRI and SalI. The DNA fragments are separated on an 0.6 % agarose gel and the 3.5 kbp EcoRI/SalI fragment is cut out and isolated by electroelution. A three fold molar excess of this fragment is ligated to pUC19 which is cut with EcoRI and SalI and treated with alkaline phosphatase.

The ligation mixture is used to transform E.coli TG1 and transformants are selected on plates containing 100 mg/l of ampicillin. Plasmid DNA is prepared from transformants and restriction analyses is performed to identify the correct construct which is designated pUCRS.

E.coli TG1 transformed with pUCRS is deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSM).

3.7. Sequencing the gene for the Major Secretion Product

(MSP)

Sequencing is done by the chain termination method (Sanger F. et al., 1977) using Sequenase® from USBC.

The 3.5 kbp EcoRI/SalI fragment prepared as described in Example 3.6 is subcloned into M13 mp18. The resulting plasmid is named M13 mpl8RS. The 2.1 kbp HindIII fragment of the 3.5 kbp EcoRI/SalI fragment is cloned into M13 mp19, the resulting plasmid is M13 mp19H. A set of unidirectional deletions is generated from the SalI end of the insert in M13 mp18RS by digesting the DNA with exonucleaseIII and SI (Henikoff, 1984; Yanisch-Perron et al., 1985). The deletions extend about 2 kbp into the insert. Sequencing these deletions from the universal mp18 primer yielded the sequence information for one strand. Based on this sequence, a set of synthetic oligonucleotides is synthesized and serves as primers to sequence the second strand. Additional sequence information is gained by sequencing exoIII-S1 deletions generated from M13 mp19H with the same method. The DNA sequence of a 1920 bp fragment of the 3.5 kbp EcoRI/SalI fragment is depicted in the sequence listing under SEQ ID No. 1. It comprises part of the MSP promoter region, the DNA sequence encoding the MSP signal peptide and the structural gene for mature MSP.

Analysis of the about 2 kbp long DNA sequence revealed the presence of a single open reading frame (ORF) coding for a protein having 461 amino acid residues. Comparison of the deduced amino acid sequence with the data obtained from sequencing the N-terminus of the isolated MSP identified a mature form of the protein with 434 amino acids which is preceded in frame by 27 amino acids. The peptide formed by the latter amino acids has the structure of a typical signal peptide. Charged residues are found at the N-terminus while the rest of the sequence consists mainly of hydrophobic amino acids. The amino acid composition around the cleavage site follows the rules deduced from other signal peptides (Von Heijne, 1983).

4. Production of recombinant Desulfatohirudin in Lactococcus

lactis

4.1 Construction of Desulfatohirudin Secretion Plasmids

Plasmid M13 mp19H contains a 2.1 kbp HindIII fragment encoding the N-terminus half of MSP including the signal peptide and about 1.5 kbp of upstream DNA. This plasmid is cleaved at its unique ScaI site 129 bp downstream of the COOH-terminus of the signal peptide. A blunt ended 211 bp DNA fragment encoding the genetic information for desulfatohirudin is isolated from plasmid pML310 (published in the European Patent Application EP-A-168 342) and inserted at the cleaved ScaI site of M13 mpl9H. An in frame fusion between the DNA sequence encoding the MSP signal peptide and the desulfatohirudin structural gene is created by removing the excess DNA separating the codon for the last amino acid of the signal peptide (Ala) and the codon for the first amino acid of desulfatohirudin (Val). This is achieved by oligonucleotide-directed in vitro mutagenesis using the system from Amersham. The 29 bp oligonucleotide comprises the last 14 bp of the signal peptide and the first 15 bp of the hirudin gene. The sequence of the resulting fusion is depicted in the sequence listing under SEQ ID No. 2.

The HindIII fragment carrying the fusion is cut out and inserted into the unique HindIII of pSC12. Both orientations of the insert are recovered. The resulting plasmids are named pSC12HIR and pSC12HIR-1, respectively.

Another plasmid (pSC12HIRTerm) is constructed by inserting the trpA transcription terminator of E.coli (Pharmacia) at the unique HpaI site of pSC12HIR about 125 bp downstream of the desulfatohirudin gene.

4.2 Secretion of Desulfatohirudin by L. lactis

The desulfatohirudin fusion plasmids are transformed into L. lactis LM 0230. Transformants are grown in M-17G medium supplemented with 2 % of glucose in the presence of erythromycin (5 mg/l) at 30°C. When the cultures reach stationary phase, 1.5 ml samples are centrifuged in an Eppendorf® tube. The culture supernatants are removed and frozen at -70°C. The cultures are then further grown overnight before a second sample is taken, centrifuged, the supernatant removed and also frozen. The production of secreted desulfatohirudin is determined with a bioassay.

The bioassay measures the thrombin inhibition activity of desulfatohirudin in the supernatants collected. A detailed protocol of the bioassay, which is a thrombin inhibition assay is presented hereinafter:

The buffer used for making all dilutions of samples and reagents is 0.2M Tris-HCl, pH 7.5 containing 1.0M NaCl and 0.01 % bovine serum albumin. Thrombin is from human plasma (Protogen AG, Läufelfingen, product No. 80-13-1102), the chromogenic thrombin substrate is Chromozyme TH (Boehringer, Mannheim, product No 206849). The p-nitroaniline released from Chromozym TH is measured with a Dynatech MR 600 microplate reader. All assays are performed in microtiter plates (Nunc, MicroWell plates). Each well receives: 50 µl buffer, 50 µl supernatant with unknown concentration of thrombin inhibitory activity, i.e. desulfatohirudin, and 25 µl thrombin solution. The reaction is started by adding 150 µl substrate solution (330 µg/ml) and the plates are incubated for two hours at 37°C. The concentration of thrombin is adjusted to give an A405nm = 0.8 ± 0.2 in an uninhibited control well. Both substrate and thrombin solutions are kept frozen at -20°C and are thawed immediately before being used. A standard curve with known concentrations of a [Tyr63]desulfatohirudin (40, 20, 10, 5, 2.5 and 1.25 ng/ml) is used to convert the OD-measurements into the concentrations of active desulfatohirudin.

In the supernatant of stationary phase cultures of L.lactis LM0230 cells transformed with either pSC12HIR or pSC12HIR-1 the same level of desulfatohirudin activity is determined. In the supernatant of a stationary phase culture of L.lactis LM0230 cells transformed with pSC12HIRTerm, the level of desulfatohirudin activity is increased by about 50 %. Supernatants of cells transformed as a control with pSC12 contain no desulfatohirudin activity.

The levels of desulfatohirudin in the supernatant did not decrease after prolonged incubation for about 16 h of the stationary phase cultures. These results indicate the absence of proteolytic degradation in the supernatant of L.lactis LM0230.

In one experiment, cells are first collected by centrifugation and then resuspended in 1/10 volume of fresh medium and incubating at 30°C for 30 min expression period. Bioassay measures an about six fold higher level of desulfatohirudin activity than in the experiments described hereinabove. In a more sophisticated form, concentration of the cells for an expression period is a way to increase the concentration of secreted product in the supernatant.

5. Production of recombinant Desulfatohirudin in Bacillus

thuringiensis

5.1 Construction of plasmid pVAHIR

Plasmid pVA838 (Macrina et al, 1982) is digested with HindIII and the 5.0 kbp fragment carrying the gene for erythromycin resistance and the gram positive origin of replication is isolated from a 0.5 % agarose gel by electroelution.

The HindIII fragment containing the desulfatohirudin gene is isolated in a similar manner from pSC12HIR.

The two fragments are ligated in the presence of T4-ligase and the ligation mixture is directly used to transform L. lactis LM 0230 by electroporation. Erythromycin resistant transformants are selected and plasmid DNA is prepared from them.

Restriction analyses of these plasmids is performed to identify correct constructions. Both orientations of the HindIII desulfohirudin expression cassette are obtained and can be used for the production of Desulfatohirudin in Bacillus thuringiensis. The plasmids are named pVAHIR and pVAHIR-1.

5.2 Secretion of Desulfatohirudin by B. thuringiensis

B. thuringiensis strain HID1 cryB (DSM 4574) is transformed with pVAHIR using electroporation (W. Schurter et al, 1989).

Transformants are selected on LB plates containing 20 µg/ml of erythromycin at 27°C or 30°C. Plasmid DNA is prepared from individual transformants by the same method as used for L.lactis. Restriction analyses reconfirms that the structure of the isolated plasmids is identical to pVAHIR.

Transformants harbouring pVAHIR or pVA838 - as a control - are grown in LB containing 20 mg/l of erythromycin at 27°C over night. The cultures are diluted 1:200 into fresh medium and grown further at 30°C. The cultures are centrifuged and the supernatants are assayed for desulfohirudin activity 7 h after dilution.

Desulfatohirudin is detected in the supernatant of cells transformed with pVAHIR, while none is measured in the control with pVA838.

Deposited Microorganisms

The following microorganisms are deposited according to the Budapest Treaty with the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSM), Mascheroder Weg 1b, D-3300 Braunschweig: <tables id="tabl0002" num="0002"><table frame="all"><tgroup cols="3" colsep="1" rowsep="0"><colspec colnum="1" colname="col1" colwidth="52.50mm" /><colspec colnum="2" colname="col2" colwidth="52.50mm" /><colspec colnum="3" colname="col3" colwidth="52.50mm" /><thead valign="top"><row rowsep="1"><entry namest="col1" nameend="col1" align="left">Microorganism</entry><entry namest="col2" nameend="col2" align="left">Deposition No.</entry><entry namest="col3" nameend="col3" align="left">Date of deposition</entry></row></thead><tbody valign="top"><row><entry namest="col1" nameend="col1" align="left">E. coli K12 TG1/pUCRS</entry><entry namest="col2" nameend="col2" align="left">DSM 5803</entry><entry namest="col3" nameend="col3" align="left">February 16, 1990</entry></row><row><entry namest="col1" nameend="col1" align="left">Lactococcus lactis LL712</entry><entry namest="col2" nameend="col2" align="left">DSM 5804</entry><entry namest="col3" nameend="col3" align="left">February 16, 1990</entry></row><row rowsep="1"><entry namest="col1" nameend="col1" align="left">Lactococcus lactis LM0230</entry><entry namest="col2" nameend="col2" align="left">DSM 5805</entry><entry namest="col3" nameend="col3" align="left">February 16, 1990</entry></row></tbody></tgroup></table></tables>

References:

<ul id="ul0005" list-style="none" compact="compact"><li>Bates, E.E.M. et al. (1989) Applied and Environmental Microbiology 55, 2095.</li><li>Bernton, W.D., and Davis, R.W. (1977), Science 196 180.</li><li>Birnboim, H.C. and Doly, J. (1979) Nucleic Acids Res., 7, 1513.</li><li>Botstein, D. and Shortle, D. (1985) Science 229, 1193.</li><li>Chassy, B.M. (1987) FEMS Microbiol. Rev. 46, 297.</li><li>Clewell, D. and Helinski, D.R. (1969) Proc. Natl. Acad. Sci. USA, 62, 1159.</li><li>Davies, F.L., Underwood, H.M. and Gasson, J.M. (1981) J. of Applied Bacteriology, 51, 325.</li><li>De Vos, W.M. (1987) FEMS Microbiol. Rev. 46, 281.</li><li>Efstathiou, J.D. and McKay, L.L. (1977) J. Bacteriol., 130, 257.</li><li>Gasson, J.M. (1983) J. Bacteriol., 154, 1.</li><li>Gasson, J.M. and Anderson P.H. (1985) FEMS Microbiol. Lett., 30, 193.</li><li>Henikoff, S. (1984) Gene, 28, 351.</li><li>Huynh et al, in: DNA Cloning 1:56-110, D.M. Glover ed., IRL Press Oxford (1985)</li><li>Jos, M. (1985) Applied and Environmental Microbiology 50, 540.</li><li>Karn, J., Brenner, S., Barnett, L., and Cesareni, G. (1980) Proc. Natl. Acad. Sci. USA, 77, 5172.</li><li>Laemmli, U.K. (1970) Nature, 227, 680.</li><li>Macrina, F.L. et al., in Genetic Engineering of Microorganisms for Chemicals, Plenum Press, New York, (1982).</li><li>Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular cloning, a Laboratory Manual. Cold Spring Harbor Laboratory, New York.</li><li>Norrander, J. et al. (1983) Gene, 26, 101.</li><li>Norris, K. et al. (1983) Nucl. Acids Res. 11, 5103.</li><li>Powell et al. (1988) Applied and Environmental Microbiology 54, 6.</li><li>Oto, R. et al. (1982) Applied and Environmental Microbiology 43, 1272.</li><li>Rusche, J.R. and Howards-Flanders, P. (1985) Nucleic Acids Res., 13, 1997.</li><li>Sanger, F. Milken, S., Coulson, A.R. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463.</li><li>Schurter, W., Geiser, M. and Mathe, D. (1989) Mol. Gen. Genet. 218, 177.</li><li>Terzaghi, B.K. and Sadine, N.E. (1975) Applied Microbiology, 29, 807.</li><li>Von Heijne, G. (1983) Eur. J. Biochem., 133, 17.</li><li>Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Gene, 33, 103.</li><li>Zoller, M.J. and Smith, M. (1983) Methods Enzymol. 100, 468.</li></ul>

Sequence Listing

<ul id="ul0006" list-style="none" compact="compact"><li>SEQ ID No. 1 <ul id="ul0007" list-style="none" compact="compact"><li>SEQUENCE TYPE: Nucleotide with corresponding protein</li><li>SEQUENCE LENGTH: 1920 base pairs</li><li>STRANDEDNESS: double</li><li>TOPOLOGY: linear</li><li>MOLECULE TYPE: genomic DNA</li><li>ORIGINAL ORGANISM SOURCE: Lactococcus lactis LM0230 (DSM 5805)</li><li>IMMEDIATE EXPERIMENTAL SOURCE: Plasmid pUCRS (DSM 5803)</li><li>POSITION IN THE GENOME: chromosomal</li><li>FEATURES: from <ul id="ul0008" list-style="none" compact="compact"><li>1 to 410 part of the promoter region</li><li>411 to 491 MSP signal peptide</li><li>492 to 1793 mature MSP protein</li></ul></li><li>PROPERTIES: Major secretion product (MSP) gene of L. lactis.</li></ul><img file="EP0449770B1_D0001.tif" /><img file="EP0449770B1_D0002.tif" /><img file="EP0449770B1_D0003.tif" /><img file="EP0449770B1_D0004.tif" /><img file="EP0449770B1_D0005.tif" /><img file="EP0449770B1_D0006.tif" /></li><li>SEQ ID No.: 2 <ul id="ul0009" list-style="none" compact="compact"><li>SEQUENCE TYPE: Nucleotide with corresponding polypeptide</li><li>SEQUENCE LENGTH: 279 bp</li><li>STRANDEDNESS: double</li><li>TOPOLOGY: linear</li><li>MOLECULE TYPE: recombinant</li><li>ORIGINAL SOURCE</li><li>ORGANISMS: L. Lactis LM0230 (DSM 5805), Hirudo medicinalis</li><li>IMMEDIATE EXPERIMENTAL SOURCE: Plasmid pUCRS (DSM 5803), plasmid pML310 (see European patent application EP-A-168 342)</li><li>FEATURES: from <ul id="ul0010" list-style="none" compact="compact"><li>1 to 81 bp MSP signal peptide</li><li>82 to 279 bp coding region for desulfatohirudin</li></ul></li><li>PROPERTIES: Fusion of the L. Lactis LM0230 DNA encoding the MSP signal peptide and of the hirudin structural gene for the production of secreted hirudin in bacteria.</li></ul><img file="EP0449770B1_D0007.tif" /><img file="EP0449770B1_D0008.tif" /></li></ul>

Every citation, both waysCites: the store holds 2 of 3

Citations
Document	Relation	Office	Cited during
EP228726A	Cites	European Patent Office (EPO)	–
EP455280A	Cites	European Patent Office (EPO)	–
FEMS MICROBIOLOGY LETTERS vol. 30, 1985, ELSEVIER. AMSTERDAM;NL pages 193 - 196; M. J. GASSON ET AL: 'High copy number plasmid vectors for use in lactic streptococci'	Non-patent	–	–
NETHERLANDS MILK AND DAIRY JOURNAL. vol. 40, no. 2/3, 1986, WAGENINGEN NL pages	Non-patent	–	–
141 - 154; W. M. DE VOS: 'Gene cloning in lactic streptococci'	Non-patent	–	–
JOURNAL OF BACTERIOLOGY vol. 154, no. 1, 1983, BALTIMORE pages 1 - 9; M. J. GASSON: 'Plasmid complements of Streptococcus lactis NCDO 712 and other lactic Streptococci after protoplast-induced curing'	Non-patent	–	–
GENE. vol. 95, September 1990, AMSTERDAM NL pages 155 - 160; M. VAN ASSELDONK ET AL: 'Cloning of usp45,a gene encoding a secreted protein from Lactococcus lactis subsp. lactis'	Non-patent	–	–
141 - 154; W. M. DE VOS: 'Gene cloning in lactic streptococci'	Non-patent	–	Examiner

32 members in 22 offices

This recordMember, by publication datePriority claim12 more offices are in the list below

Priority claims5

Priority claims
Document	Office	Kind	Date
9006400	United Kingdom	A	1990-03-22
9006400	United Kingdom	A	1990-03-22
9006400	United Kingdom	–	1990-03-22
9006400	–	–	–
GB19900006400	–	–	–

Members32

Family members, oldest first
Document	Office	Kind
GB9006400D0	United Kingdom	D0	1990-05-23
NO911138D0	Norway	D0	1991-03-21
CA2038706A1	Canada	A1	1991-09-23
FI911367A	Finland	A	1991-09-23
FI911367A7	Finland	A7	1991-09-23
FI911367L	Finland	L	1991-09-23
NO911138L	Norway	L	1991-09-23
IE910948A1	Ireland	A1	1991-09-25
EP0449770A2	European Patent Office (EPO)	A2	1991-10-02
AU7355891A	Australia	A	1991-10-03
HU910958D0	Hungary	D0	1991-10-28
BR9101137A	Brazil	A	1991-11-05
KR910016930A	Republic of Korea	A	1991-11-05
HUT57263A	Hungary	A	1991-11-28
PT97081A	Portugal	A	1991-11-29
ZA912114B	South Africa	B	1992-01-29
EP0449770A3	European Patent Office (EPO)	A3	1992-07-01
JPH04211384A	Japan	A	1992-08-03
NZ237506A	New Zealand	A	1992-12-23
TW213488B	Taiwan Province of China	B	1993-09-21
AU643511B2	Australia	B2	1993-11-18
MX25027A	Mexico	A	1993-12-01
AR245504A1	Argentina	A1	1994-01-31
US5559007A	United States of America	A	1996-09-24
PT97081B	Portugal	B	1998-07-31
EP0449770B1This record	European Patent Office (EPO)	B1	1998-12-02
AT174055T	Austria	T	1998-12-15
ATE174055T1	Austria	T1	1998-12-15
DE69130544D1	Germany	D1	1999-01-14
ES2125864T3	Spain	T3	1999-03-16
DE69130544T2	Germany	T2	1999-04-29
DK0449770T3	Denmark	T3	1999-06-23

58 legal events, as 6 offices reported them to INPADOC

Over the term

Point at a mark for the event

Events

Legal events, newest first
	Event	Code	Office
2005-03-13	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2004-04-01	Announcement of lapse in spainLapsedFD2A	FD2A	ES
2003-01-10	Notification of lapseLapsedST	ST	FR
2002-12-02	Nl: lapsed or anulled due to non-payment of the annual feeLapsedNLV4	NLV4	EP
2002-11-29	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-11-25	Ep patent lapsedLapsedEBP	EBP	DK
2002-11-15	Patent ceasedCeasedPL	PL	CH
2002-11-06	Gb: european patent ceased through non-payment of renewal feeCeasedGBPC	GBPC	EP
2002-10-22	Se: european patent has lapsedLapsedEUG	EUG	EP
2002-10-01	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-10-01	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-09-30	Be: lapsedLapsedBERE	BERE	EP
2002-04-02	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-03-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-03-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-03-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-03-14	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-03-14	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-03-13	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-03-13	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-01-01	European patent in force as of 2002-01-01IF02	IF02	GB
2001-05-16	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-30	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-15	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-13	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-13	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-13	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-07	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-06	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-03-06	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
2001-02-28	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-11-24	No opposition filedOpposition26N	26N	EP
1999-10-08	No opposition filed within time limitOppositionORIGINAL CODE: 0009261PLBE	PLBE	EP
1999-10-08	Information on the status of an ep patent application or granted ep patentGrantedSTATUS: NO OPPOSITION FILED WITHIN TIME LIMITSTAA	STAA	EP
1999-06-23	Ep patent with danish claimsT3	T3	DK
1999-03-16	Definitive protectionFG2A	FG2A	ES
1999-03-13	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1999-02-12	It: translation for a ep patent filedITF	ITF	EP
1999-01-22	Fr: translation filedET	ET	EP
1999-01-14	Corresponds to:REF	REF	EP
1998-12-15	European patent takes effect as a national patent in ch/liEP	EP	CH
1998-12-02	Designated contracting statesAK	AK	EP
1998-12-02	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1998-12-02	Corresponds to:REF	REF	EP
1998-10-16	(expected) grantORIGINAL CODE: 0009210GRAA	GRAA	EP
1998-10-01	Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOS IGRAGRAH	GRAH	EP
1998-09-23	Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOS IGRAGRAH	GRAH	EP
1998-04-07	Despatch of communication of intention to grantORIGINAL CODE: EPIDOS AGRAGRAG	GRAG	EP
1998-04-07	Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOS IGRAGRAH	GRAH	EP
1997-09-26	Despatch of communication of intention to grantORIGINAL CODE: EPIDOS AGRAGRAG	GRAG	EP
1997-05-14	Party data changed (applicant data changed or rights of an application transferred)RAP1	RAP1	EP
1997-04-02	Party data changed (applicant data changed or rights of an application transferred)RAP1	RAP1	EP
1995-04-26	First examination report despatched17Q	17Q	EP
1992-07-01	Designated contracting statesAK	AK	EP
1992-05-08	Search report despatchedORIGINAL CODE: 0009013PUAL	PUAL	EP
1991-10-02	Request for examination filed17P	17P	EP
1991-10-02	Designated contracting statesAK	AK	EP
1991-08-15	Public reference made under article 153(3) epc to a published international application that has entered the european phaseORIGINAL CODE: 0009012PUAI	PUAI	EP

Bacterial vectors

Abstract

Term

(MSP)

Priority claims5

Members32

Over the term

Events

Numbers

Titles3

Classification

Designated states1