Development of dna probes and immunological reagents of human tumor associated antigens

(-- A summary 57) The method of manufacturing the immunological reagent as for which * carries out the cell surface antigen of the (correction -- owner) subject human origin is offered. Solution means DNA as for which * carries out the character which can be identified so that DNA isolated from human tumor cells and selection are possible is introduced into the established non-human tumor formative-cells system, Choose and collect transduced cells and this is poured into the 1st suitable rat host, The poured-in transduced cell is induced and the obtained rat host is maintained during the period effective in forming a tumor in a rat host, Obtain tumor cells from the obtained tumor, solid-phase-ize the antiserum generated to that tumor cell to the non-human non-tumor formative-cells system established above-mentioned, pour an antiserum solid phase-ized cell into the 2nd suitable host, and this host is screened, Extraction and hype ーマ are adjusted for a spleen from the 2nd host who identified the host who generates the serum reacted to human tumor cells, and was identified by making it such, and the hype ーマ cell lines which produce the antibody which recognizes a cell surface antigen specifically and is combined are collected.