Dna sequencing by mass spectrometry via exonuclease degradation

(57) エキソ nuclease activity cuts summary nucleic acid from the first end to one way, The determination method of the nucleic acid arrangement by identifying each nucleotide which makes emit each nucleotide to arrangement order, and is emitted to arrangement order with a mass spectrometry machine, and determining nucleic acid arrangement from the identified nucleotide is indicated. Multiplexing for determining one or more nucleic acid arrangement simultaneously is possible for this method.