Three-dimensional cell and tissue culture system
Abstract
A three-dimensional cell culture system which can be used to grow different cells and tissues in prolonged periods of time is described. Cells derived from a single tissue are inoculated and grown on a pre-established stromal carrier matrix. The stromal matrix comprises stromal cells, such as fibroblasts which actively grow on a three-dimensional matrix. The stroma cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages / monocytes, adipocytes, perlytes, reticular cells found in bone marrow stroma, etc. The stromal matrices carry the carrier, growth factors and regulatory factors necessary for sustained long-term active proliferation. of cells in culture. When grown in this three-dimensional system,
Term
No projected expiry on record.
- Priority
- Filed
- Published
- Today
1 claim: 1 independent, 0 dependent
- 1components to adult tissue analogous to the counterparts found in vlvo. ponenter tll voksent vev analogt med motpartene som finnes in vlvo.
6 paragraphs, as filed
(21) Application number 910787 (22) Inception date 27.02.91 (24) Training day 07.09.89 (41) Alm. avail. 22.04.91 (62) Initial application (83) (86) Int. Entry date and application number
07.09.89, PCT / US89 / O3853 (85) Continuation day 27.02.91 (30) Priority 08.09.88, US, 242096
01.09.89, US, 402104 (71/73) Applicant / Holder Marrow-Tech Inc., 100 Grasslands Road, Elmsford, NY 10523, US (72) Inventor (G) K. Naughton, Yorktown Heights, NY, US
Brian A. Naughton, Yorktown Heights, NY, US (74) Proxy Bryns Patentkontor AS, Oslo (54) Designation Threedimensjonalt celle- og vevskultursystem.
(57) Sammendrag
Et tredimensjonalt cellekultUrine System That Can Be Used To Grow Different Cells And Tissues In vitro 1 extended time periods are described. Cells derived from a desired tissue are inoculated and grown on a pre-established stromal carrier matrix. The straw carrier matrix includes stromal cells, such as fibroblasts which actively grow on a three-dimensional matrix. The stromal cells may also include other cells present in loose connective tissue such as endothelial cells, macrophages / monocytes, adipocytes, perlytes, reticular cells found in the bone marrow stroma, etc. The stromal matrices provide the carrier, growth factors and regulatory factors necessary for sustained long-term active proliferation of the cells 1 culture. When cultured in this three-dimensional system, the proliferating cells mature and segregate properly to form the
157 members in 31 offices
Priority claims10
| Document | Office | Kind | Date |
|---|---|---|---|
| 24209688 | United States of America | A | |
| 24209688 | United States of America | A | |
| 40210489 | United States of America | A | |
| 40210489 | United States of America | A | |
| 8903853 | United States of America | W | |
| 8903853 | United States of America | W | |
| 89402104 | – | – | – |
| US19880242096 | – | – | – |
| US19890402104 | – | – | – |
| WO1989US03853 | – | – | – |
Members157
| Document | Office | Kind | |
|---|---|---|---|
| IE871007L | Ireland | L | |
| EP0241578A2 | European Patent Office (EPO) | A2 | |
| AU5985086A | Australia | A | |
| WO8706120A1 | World Intellectual Property Organization (WIPO) | A1 | |
| JPS62249926A | Japan | A | |
| AU7356887A | Australia | A | |
| DK665687A | Denmark | A | |
| DK665687D0 | Denmark | D0 | |
| NO875286D0 | Norway | D0 | |
| NO875286L | Norway | L | |
| US4721096A | United States of America | A | |
| PT87136A | Portugal | A | |
| KR880701093A | Republic of Korea | A | |
| IL85957D0 | Israel | D0 | |
| FI884783A | Finland | A | |
| GR880100216A | Greece | A | |
| EP0309456A1 | European Patent Office (EPO) | A1 | |
| HUT48112A | Hungary | A | |
| EP0309456A4 | European Patent Office (EPO) | A4 | |
| EP0241578A3 | European Patent Office (EPO) | A3 | |
| BR8707673A | Brazil | A | |
| ES2009894A6 | Spain | A6 | |
| JPH01503195A | Japan | A | |
| EP0358506A2 | European Patent Office (EPO) | A2 | |
| WO9002796A1 | World Intellectual Property Organization (WIPO) | A1 | |
| PT91676A | Portugal | A | |
| AU4211489A | Australia | A | |
| AU595813B2 | Australia | B2 | |
| EP0358506A3 | European Patent Office (EPO) | A3 | |
| NZ218282A | New Zealand | A | |
| ZA896886B | South Africa | B | |
| NZ220038A | New Zealand | A | |
| US4963489A | United States of America | A | |
| KR900702006A | Republic of Korea | A | |
| NO910787D0 | Norway | D0 | |
| DK40591D0 | Denmark | D0 | |
| AU6815990A | Australia | A | |
| AU6816090A | Australia | A | |
| HU202578B | Hungary | B | |
| CA1282725C | Canada | C | |
| NO910787LThis record | Norway | L | |
| DK40591A | Denmark | A | |
| US5032508A | United States of America | A | |
| HU895973D0 | Hungary | D0 | |
| BR8907642A | Brazil | A | |
| HUT56393A | Hungary | A | |
| HU910750D0 | Hungary | D0 | |
| AU615414B2 | Australia | B2 | |
| JPH04501657A | Japan | A | |
| US5160490A | United States of America | A | |
| PT87136B | Portugal | B | |
| CA1310926C | Canada | C | |
| BG51337A3 | Bulgaria | A3 | |
| RO106655B1 | Romania | B1 | |
| US5266480A | United States of America | A | |
| AU644578B2 | Australia | B2 | |
| NZ230572A | New Zealand | A | |
| IL85957A | Israel | A | |
| CA1335657C | Canada | C | |
| US5443950A | United States of America | A | |
| EP0309456B1 | European Patent Office (EPO) | B1 | |
| AT127692T | Austria | T | |
| ATE127692T1 | Austria | T1 | |
| DE3751519D1 | Germany | D1 | |
| US5460939A | United States of America | A | |
| CA2189882A1 | Canada | A1 | |
| WO9531532A1 | World Intellectual Property Organization (WIPO) | A1 | |
| IL114017D0 | Israel | D0 | |
| AU2549595A | Australia | A | |
| CA2192064A1 | Canada | A1 | |
| WO9533821A1 | World Intellectual Property Organization (WIPO) | A1 | |
| AU2769695A | Australia | A | |
| DE3751519T2 | Germany | T2 | |
| US5510254A | United States of America | A | |
| US5512475A | United States of America | A | |
| NO179181B | Norway | B | |
| US5516680A | United States of America | A | |
| US5516681A | United States of America | A | |
| US5518915A | United States of America | A | |
| US5541107A | United States of America | A | |
| NO179181C | Norway | C | |
| IL91536A | Israel | A | |
| FI964592A | Finland | A | |
| NO964862D0 | Norway | D0 | |
| US5578485A | United States of America | A | |
| US5580781A | United States of America | A | |
| CA2224071A1 | Canada | A1 | |
| WO9640175A1 | World Intellectual Property Organization (WIPO) | A1 | |
| AU6031596A | Australia | A | |
| NO964862L | Norway | L | |
| HU9603155D0 | Hungary | D0 | |
| EP0757717A1 | European Patent Office (EPO) | A1 | |
| PL317234A1 | Poland | A1 | |
| US5624840A | United States of America | A | |
| CN1152935A | China | A | |
| KR970703417A | Republic of Korea | A | |
| CZ336696A3 | Czechia | A3 | |
| SK147696A3 | Slovakia | A3 | |
| HUT76354A | Hungary | A | |
| BR9507657A | Brazil | A |
Numbers
- Publication, DOCDB
- 910787
- Publication, EPODOC
- NO910787L
- Application
- 91910787
- Application, DOCDB
- 910787
- Application, EPODOC
- NO19910000787
Titles2
- Norwegian
- TREDIMENSJONALT CELLE- OG VEVSKULTURSYSTEM.
- English
- THREE-DIMENSIONAL CELL AND WEIGHT CULTURE SYSTEM.
Classification
- CPC, 25
- C12N5/0062
- A61L27/38
- A61L27/60
- A61K35/12
- C12N5/0618
- C12N5/0669
- C12N5/0671
- C12N5/0677
- C12N5/0697
- C12N5/0698
- A61K2035/124
- C12N2502/081
- C12N2502/086
- C12N2502/091
- C12N2502/094
- C12N2502/097
- C12N2502/1305
- C12N2502/1323
- C12N2502/28
- C12N2502/30
- C12N2503/04
- C12N2510/00
- C12N2533/30
- C12N2533/40
- C12N2533/70
- IPC, 21
- A01N1 02
- A01N63 02
- A61K35 12
- A61K49 00
- A61L27 00
- C12N15 02
- A61L27 38
- A61L27 60
- C12M3 00
- C12N5 00
- C12N5 02
- C12N5 071
- C12N5 10
- C12N11 02
- C12N15 09
- C12Q1 02
- C12Q1 18
- C12Q1 24
- G01N33 15
- G01N33 48
- G01N33 50