Fungicidal preparation and method of funguses control

The present invention relates to combinations of novel biologically active substances for combating plant diseases in mixtures and methods of applying such mixtures to leaves, soil treatments and seed treatments.

The combinations of the invention have the following components:

a) 2- (4-Chlorophenyl) -3-cyclopropyl-1- (1H-1,2,4-triazoll-yl) butan-2-ol of Formula I

<img file="LT3728B_D0001.tif" />

or acid salts thereof and metal complexes thereof, and

b) cis -4- [3- (4-tert-butylphenyl) -2-methylpropyl] -2,6-dimethylmorpholine of Formula II

<img file="LT3728B_D0002.tif" />

<img file="LT3728B_D0003.tif" />

or an acid addition salt thereof and / or ΙΕ 3- (4-tert-butylphenyl) -2-methylpropyl] -ciperidine of formula III

<img file="LT3728B_D0004.tif" />

(III) or its salts obtained by acid addition.

Component a) is known by the name cyproconazole. Its synthesis and fungicidal properties are described in patent application VFR patent no. 3406993.

Component b) is known as fenpropimorph and component c) is known as phenpropidin. Their synthesis and fungicidal properties are described in the patent application VFR patent no. 2752135.

Unexpectedly, it has been found that the combinations of biologically active substances of the invention have a significantly higher fungicidal activity than the sum of the activities of the individual substances. This indicates that there is an unexpected synergistic increase in action, rather than the simple enhancement that would be expected when the two components are mixed. The combinations of biologically active substances proposed in the present invention also enrich the prior art.

In the case of biologically active substances in the combinations of the invention in which these substances are taken in defined weight ratios, the synergistic effect is particularly pronounced. However, depending on the applications, the weight ratios of the biologically active substances in their mixtures can be varied within a relatively wide range. In general, 0.2 to 20 parts by weight, preferably 0.5 to 10 parts by weight, of active ingredient of formula (II) and / or (III) are present per part by weight of the compound of formula I. This corresponds to a weight ratio of components a) and b) of 5: 1 to 1:20.

Particularly suitable are combinations containing from 1 to 8 parts by weight of the compound of formula (I) containing from 1 to 8 parts by weight of the active ingredient (s) of formula (II) and / or III, preferably when a): b) = from 2: 1 to 1:10 and best results with a ratio of 1: 1 to 1: 4. Examples for use in practice may be referred to as the most suitable proportions of the components in the mixture, namely: a): b) = 2: 3, 2: 5, 1: 2 and 1: 3. The same quantitative ratios are used for salts obtained with acid addition or for metal complexes of biologically active substances.

Among the acids which may be used to form the salts of the compounds of the formulas I, II or III, the following should be noted: halogenated hydrocarbons such as hydrobromic acid and hydrochloric acid, in addition to phosphorus, nitric and sulfuric acid, in addition to mono, bi and trifunctional carboxylic and hydrocarboxylic acids such as formic, succinic, acetic, glycolic, fumaric, lactic, oxanyl, propane, sorbin, trichloroacetic acid, trifluoroacetic acid, citric acid and the like, sulfuric acids such as benzenesulfo-1,5-naphthalenesulfonic and p-toluenesulfonic acids as well as (thio) saccharin.

The metal complexes consist of a basic organic molecule and an inorganic or organic metal salt which may be selected, for example, from halogen, nitrate, sulfate, phosphate, acetate, trifluoroacetate, trichloroacetate, propionate, tartrate, sulfonate, salicylate, benzoate, etc. The cation (metal) of these salts may be from the second basic group of elements, such as aluminum, tin or lead, as well as from the first to eighth side groups such as chromium, manganese, iron, cobalt, nickel, copper, zinc. etc. Elements of the fourth period by-groups should be given priority. Metals can be of various valences, which are characteristic of them.

The mixtures of biologically active substances and their salts obtained by the addition of acids according to the invention have a fungicidal effect on plants and can therefore be used to control fungi found in agriculture and horticulture. In particular, they are suitable for slowing down or destroying the growing phytopathogenic fungi of individual parts of plants, such as leaves, stems, roots, tubers, fruits or flowers, and for controlling harmful fungi occurring in the soil.

Mixtures of biologically active substances according to the invention are most suitable for combating ascomycetes (brysiphe graminis, Uncinula necator, Venturia, Sphaerotheca pannosa, brysipne betae) and basidiomycetes characterized by rust diseases belonging to Puccinia, Uromyces and Hemilcia species (including Puccinia recondi, Puccinia graminis, Puccinia coronata, Uromyces fabae, Uromyces appendiculatus, Hemilcia Vastatrix).

Further, the combinations of biologically active agents of the invention are effective against the diseases Helminthosporium of the species Fungiimperfecti (e.g. Helminthosporium oryzae, Helminthosporium teres, Helminthosporium sativum, Helminthosporium tritici-repentis), Alternaria (e.g. Alternaria brassiola, Alternaria br

Septoria (e.g. Septoria (e.g. Ceratocystis ulmi), acenae), Cercospora Pyricularia (e.g. Pyricularia oryzae and Mycosphaerella fijiensis).

The combinations of biologically active substances of the invention are suitable, inter alia, for the control of fungal strains which have become resistant to the action of triazole class of biologically active substances.

Single doses used in the open area range from 75 to 1000 g of active substance per hectare. For combating seed fungi, it is preferable to use a dose of 0.01 to 1.0 g of active ingredient mixture per kilogram of seed. It would be logical to apply the same amounts to the substances used for plant propagation, ie converted to 1 kg of sprouts, tubers, root shoots, etc.

The fungicidal compositions of the invention have systemic, therapeutic and prophylactic effects.

Mixtures of biologically active substances of the invention may be processed into various types of devices, such as solutions, suspensions, emulsions, emulsifiable concentrates and powder formulations. Such fungicidal agents are the subject of the present invention. The fungicidal compositions of the present invention are characterized in that they contain effective amounts of ciproconazole and phenpropimorph and / or phenpropidin or salts of the acid addition or metal complexes of these active substances as well as excipients. It is desirable to add at least one excipient from the group consisting of solid fillers, solvents or dispersants, surfactants (woolen and emulsifiers), dispersants (non-surfactant) and other types of additives such as stabilizers.

The most commonly used solid aggregates are: natural minerals such as kaolin, chalk such as flaked chalk, magnesium carbonate, limestone, quartz, dolomite, atapulgite, montmorillonite and diatomite, synthetic minerals such as highly dispersible silicas, alumina and silicas substances such as cellulose, starch, urea and synthetic resins and fertilizers such as phosphates and nitrates, these fillers can be used in both granular and powder form.

Suitable solvents or dispersants are aromatic compounds such as toluene, xylenes, high alkylation benzenes and alkylnaphthalenes, chlorinated aromatic compounds and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes and methylene chloride, (cyclic) aliphatic hydrocarbons, such as petroleum fractions, alcohols such as butanol and glycol as well as their ethers and esters, ketones, such as acetone, methyl ketone, methyl isobutyl ketone, isophorone and cyclohexane, and highly polar solvents and dispersants such as dimethylformamide, n-methylpyrrolidone and dimethylsulfoxide are also preferred to have a flash point of at least 30 ° C and a boiling point of such solvents. like 50 ° C, and water. Solvents and dispersants also include so-called liquefied gas diluents or fillers. These are products that are in gaseous form at room temperature and pressure. When water is used as a solvent, organic solvents may be used as an additional solvent.

Surfactants (wolfguns and emulsifiers) may be non-ionic compounds such as condensation products of fatty acids, saturated alcohols or ethylene oxide products of fatty acid-substituted phenols, ethers and esters of fatty acids and sugars or polyhydric alcohols, and products obtained by condensation of sugars or polyhydric alcohols. ethylene oxide alcohols, ethylene oxide and propylene oxide block copolymers or alkyldimethylamine oxides.

Surfactants may also include anionic compounds such as soaps, fatty sulfate esters such as dodecyl sodium sulfate, octadecyl sodium sulfate and acetyl sodium sulfate, alkyl sulfonates, aryl sulfonates and saturated aromatic sulfonates such as alkylbenzenesulfonate and butyl naphthalene, and for example, amidocondensation products of butyric acid and N-methylturin and sodium sulfate of dioctylsuccinate.

Finally, cationic compounds such as alkyldimethylbenzylammonium chloride, dialkyldimethylammonium chloride, alkyltrimethylammonium chloride and ethoxylated quaternary ammonium chloride may also be present as surfactants.

Dispersants (non-surfactant) include (mainly): sodium or ammonium salts of lignosulfonic acid, sodium salts of copolymers of anhydride and diisobutylene, sodium and ammonium salts of polycondensation products of naphthalene and formaldehyde, sodium carboxylic acids of polymers and carboxylic acids. Dispersants which serve as thickeners or anti-precipitants include, for example, methylcellulose, carboxymethylcellulose, hydroxymethylcellulose, polyvinyl alcohol, alginates, caseinates and blood albumin.

Examples of suitable stabilizers are acid-binding agents such as epichlorohydrin, phenylglycid ether and soy epoxides, antioxidants, gallic acid esters and butylhydroxytoluene, UV-absorbers such as benzo-phenones, diphenylalkylnitranilic acid esters and cinnamic acid esters and cinnamic acid esters, and polyglycols.

The fungicidal compositions of the invention may contain, in addition to the combinations mentioned above, other active substances, such as other fungicidal agents [active substances c) and d)], insecticidal and acaricidal agents, bactericides, plant growth regulators and fertilizers. Such combined measures allow broadening of the spectrum of action or have other positive effects on plant growth.

In general, the fungicidal compositions of the invention contain from 0.0001 to 95% by weight of the active ingredients, depending on the type. Concentrates of active substances are present in the upper part of the concentration range. Concentrates may be further diluted with one or more types of excipients to obtain concentrations of the active substances which are suitable for practical use and are usually at the lower end of the concentration range indicated above. The emulsifiable concentrates (in general) contain from 5 to 95% by weight, preferably from 25 to 85% by weight, of active substances. In practice, they can be used, among other forms, in the form of solutions, emulsions and suspensions, which may be used, for example, for spraying plants. Such solutions may contain, for example, from 0.0001 to 20% by weight of active substances. In the case of the Ultralow-Volume spraying solution, the spray solutions are prepared in a concentration of 0.5 to 20% by weight, whereas in the case of LowVolume and High-Volume spray solutions, the concentration of the active substances should preferably be 0, 02 to 1.0 or 0.002 to 0.1% by weight.

The fungicidal compositions of the invention may be prepared by mixing the active ingredient combinations of the invention with excipients.

These agents may be prepared by known methods, for example, by thoroughly mixing the active substances with solid excipients, dissolving or suspending them in suitable solvents or dispersants, and, where appropriate, acting as wetting or emulsifying or dispersing surfactants, diluting pre-formulated emulsifiers with solvents or dispersants. concentrates and so on

In the case of powder formulations, the active ingredients may be mixed with the solid excipient, for example by co-grinding, or the solid excipient may be impregnated with a solution or suspension of the active ingredients, followed by removal of the solvent or dispersant by evaporation, heating or suction. The addition of surfactants or dispersants to such powdered formulations enables them to be readily absorbed in water and made into suspensions in water, for example as a spraying agent.

The active ingredient mixtures of the present invention may also be mixed with a surfactant and a solid filler to give a water-dispersible powder which is dispersible in water, or they may be mixed with a granular filler to give a product in granular form.

If desired, the active ingredient mixtures of the invention may be dissolved in a water immiscible solvent such as an alicyclic ketone containing a dissolved emulsifier. Such a solution, when poured into water, will have a self-absorbing capacity. On the other hand, the active ingredient combinations can be mixed with an emulsifier and then diluted with water to the desired concentration. Mixtures of active substances may also be dissolved in a solvent and then mixed with an emulsifier.

Such a mixture may also be diluted with water to the required concentration. In this way, emulsifiable concentrates or ready-to-use emulsions are obtained.

Fenpropimorph Ciproconazole

N-methylpyrrolidone (co-solvent)

The methods of the invention can be used in practice using conventional techniques in plant protection technology and agriculture. The inventive method of controlling harmful fungi is characterized by treating the crop area or the seed material, such as the plants themselves, parts thereof or seeds, with an effective amount of the active ingredient combination or the inventive agent.

Where appropriate, the fungal lesion or injury site is treated in any order or at the same time with active substance I or salt or complex and / or active substance III or salt thereof.

EXAMPLES OF MIXTURES Example: Emulsifiable Concentrate (EC)

300 g / 1 50 g / 1

100 g / 1

Nonylphenol polyethoxylate (nonionic emulsifier) ​​50 g / l Ca-dodecylbenzenesulfonate (anionic emulsifier) ​​25 g / l Alkylbenzene mixture (solvent) up to 1000 ml

This type of concentrate can be diluted with water until solutions for leaves, other parts of the plant and soil treatment are obtained.

example. Emulsifiable Concentrate (EC)

Fenpropimorph '120 g / l

Phenpropidin 240 g / L

N-methylpyrrolidone (cosolvent) 100 g / l

Isotridecanol polyethoxyl (nonionic emulsifier) ​​75 g / l

Ca-dodecylbenzenesulfonate (anionic emulsifier) ​​25 g / l

Alkylnaphthalene mixture (solvent) up to 1000 ml

All components dissolve by stirring, and mild heating accelerates the dissolution process. Such solutions, when diluted, are used to prevent fungal deposition of plants or parts thereof (seeds, seedlings, tubers, etc.).

3 example. Emulsifiable Concentrate (EC)

<td>Fenpropimorph</td><td> 50</td><td>g / 1</td>

<td>Fenpropidin</td><td> 50</td><td>g / i</td>

<td>Ciproconazole</td><td> 25</td><td>g / i</td>

<td>Octylphenolpolyethylene glycol ether (4-5 mo.</td><td></td><td></td>

<td>ethylene oxide)</td><td> 30</td><td>g / i</td>

<td>Ca-dodecylbenzenesulfonate</td><td> 30</td><td>g / i</td>

Castor oil polyglycol ether (35 moles ethylene oxide)

Cyclohexanone g / 1 g / 1

Xylol mixture up to 1000 ml

The solvents obtained in Examples 1-3 are emulsified in water to give a final solution of the desired concentration for spray application. This solution can be used, for example, to strain cereal seeds.

Example: WP

Fenpropimorph 25% Ciproconazole 25% Hydrated Silica (Silica Filler) 25% Nonylphenol Polyethoxylate (Wool) 4% Na-Polycarboxylate (Dispersant) 4% Calcium Carbonate (Inert Material, Filler) 17%

The first step in the preparation of this wettable powder is to mix phenpropidine and nonylphenol polyethoxylate and spray the mixture onto the silica present therein.

Other components are then added and thoroughly ground, for example, in a blow-disk mill.

Mixing the resulting wettable powder with water produces a finely dispersed suspension of the desired dilution, which can serve as a ready-to-use solution, for example, for seedling plant seedlings, such as tubers, roots and foliage or for seed dressing.

Example: WP

Fenpropimorph 15% Fenpropidin 25% Ciproconazole 10% Na-lignin sulphonate 5%

Na-Diazobutyl Naphthalenesulfonate 6%

Octylphenolpolyethylene glycol ether (7-8 mol

2 ethylene oxide) °

Highly dispersed silica 10%

Kaolin 27% Example: WP

Fenpropimorph 50%

Fenpropidin 20%

Ciproconazole 5%

Na-Lauryl Sulfate 5%

Na-Diazobutyl Naphthalenesulfonate 10%

Highly dispersed silica 10% and the active ingredients of Example 6 are well mixed with additives and thoroughly ground in a mill. A wettable powder is obtained, which can be diluted with water to give the desired concentration of suspension.

example. In the form of dust

Fenpropimorph 15% Ciproconazole 10% Kaolin · 87% Highly dispersed silica 5%

The ready-to-use dusting agent is prepared by mixing the active substance with a filler and grinding in a suitable mill.

Biological samples

B-1. Fungal growth test on Helmint hosposium repentis-tritici.

(a) Method

The fungal strain is cultivated for 7 days at 18 ° C under artificial daylight for 16 h / day. The strain is grown on potato dextrose-agar (PDA) containing one or both of the active substances or on agar devoid of active substances (control). For this purpose, each of the active substances I and II is dissolved in pure ethanol and the solutions are mixed in the desired quantitative ratios and diluted. The defined amount of the mixture is then placed in a liquid PDA medium at 50 ° C and mixed thoroughly. Agar media at concentrations of 30, 10, 3, 1, 0, 3, 0.1, 0.03 and

0.01 mg active substance / l. The concentration of ethanol in the medium is in all cases 0.1%.

The liquid medium is then poured into Petri dishes (0 9 cm) and infected by placing an agar cylinder (0 5 cm) cut from a 7-day-old fungal culture in the center of the dish. The infected plates are incubated in a dark climatic chamber for 5 days at 18 ° C. Each test is repeated 3-4 times.

(b) Evaluation

At the end of the incubation period, the diameter of the fungal colony is measured. The fungicidal effect shall be transferred to

Probit values ​​according to Abbot (Bliss CI, Ann. Appl. Biol. 22, 134-167 (1935) and are plotted against the dose-effect versus logarithm of fungicidal concentrations. The dose-effect curve is plotted versus the probit-log curve. (DL Finey 1971 Probit analysis 3rd ed., Cambridge, UK:

Cambridge University Press). From this line linear regression and ED-50 (Effective Dosing) values ​​are determined.

(c) Calculation of the fungicidal factors (SF) in the mixture.

Theoretical effect of a mixture (ED_th) can be calculated using Uodli [2] Wadley, FM (1945),

The Widence is required to show synergistic action of insecticides and a short mt in analysis.

Et-223 US Department of Agriculture, 8 pp. Wadley FM (1967) Experimental statistics in entomology. Washington, USA (Graduate School Press., USDA), if the ED-values ​​of the individual components of the mixture are known.

a + b

ED-50 (th) = ab

- j-ED-50a ED-50b a, b = ratio in the mixture of fungicides

The ratio of theoretically calculated effect (EDth) to actual observed mixture effect (EDob) is the synergistic factor (SF).

ED-50 (th)

SF

ED-50 (ob)

SF> 1.2 synergistic interaction,

SF> O, 5 <1.2 additive interaction,

SF &lt;0.5 antagonistic interaction.

According to [3]: V. Giži et al. V. Gisi, V. Binder, H. Rimbach, E (1985).

Synergetic interactions of fungicides with different modes of action

Trans. Br. mycal. Soc. 85 (2), 299-306 and J. Levi et al. Disc. Y. et. al. (1986)

The joint action of fungicides in mixture: a comparison of two methods of synergycalculation

Bulletin OEPP 16, 651-657 (1986), SF values ​​greater than 1.0 already show synergistic interactions.

The limits of the synergistic factor for a given mixture are determined using the standard deflection of the ED values. SF-values ​​greater than 1.2 indicate characteristic statistical synergism.

(d) Results of tests on active substances I and II.

Activity of the individual components and mixture (ED-50)

<td rowspan="2">Active substance</td><td colspan="4">Test #</td>

<td> 1</td><td> 2</td><td> 3</td><td> 4</td>

<td>I</td><td> 9,0</td><td> 30, 9</td><td> 13, 1</td><td> 12,9</td>

<td>II</td><td> 2,5</td><td> 1,9</td><td> 1,9</td><td> 0, 8</td>

<td>I: II = 1: 1</td><td> 1,8</td><td> 1,9</td><td> 2,3</td><td> 0,8</td>

<td>SF-values ​​ED-50</td><td> 2,2</td><td> 1,9</td><td> 1,4</td><td> 1,9</td>

<td> [ 2]</td><td></td><td></td><td></td><td></td>

(e) Results of tests on active substances I and III

Mixed Component Activity (ED-20)

<td rowspan="2">Active substance</td><td colspan="4">Test #</td>

<td> 1</td><td> 2</td><td> 3</td><td> 4</td>

<td>I</td><td> 9,0</td><td> 30, 9</td><td> 13,1</td><td> 12,9</td>

<td>II</td><td> 3, 6</td><td> 3,2</td><td> 2,8</td><td> 0, 8</td>

<td>I: III = I: I</td><td> 1,9</td><td> . 2,3</td><td> 1,6</td><td> 0, 6</td>

<td>SF-values ​​ED-50</td><td> 2,7</td><td> 2,5</td><td> 2,9</td><td> 2, 5</td>

<td> [ 3]</td><td></td><td></td><td></td><td></td>

B-2. Fungal growth test on Septonia nodorum

The test method and its rating are the same as in biological sample Bl.

(a) Results of active tests I and II

Activity of the individual components and mixture (ED-50)

(b) Results of active tests I and III

Table of activity of individual components and mixture (ED-50)

<td rowspan="2">Active material</td><td colspan="2">Test #</td>

<td> 1</td><td> 2</td>

<td>I</td><td> 0,7</td><td> 0, 3</td>

<td>II</td><td> 0,8</td><td> 0,4</td>

<td>I: II = I: I</td><td> 0,4</td><td> 0,1</td>

<td>SF values ​​ED-50</td><td> 1,9</td><td> 3,4</td>

table

<td rowspan="2">Active material</td><td colspan="2">Test #</td>

<td> 1</td><td> 2</td>

<td>I</td><td> 0,7</td><td> 0,3</td>

<td>III</td><td> 1,4</td><td> 1,3</td>

<td>I: III = I: I</td><td> 0,7</td><td> 0,3</td>

<td>SF values ​​ED-50</td><td> 1,3</td><td> 1,6</td>

(f) Comments

The values ​​of Tables 1 to 4 for each of the two or four independent processes indicate that the fungicidal activity of both the active substance I and II mixture and the active substance mixture I and III mixture is significantly increased, i.e. synergistically increased. The results of this effect appear to be reproducible at any time.

Similar results are obtained for Helminthosporium tenes.