Novel nucleic acid prodrugs and method of use thereof

COMPLETE SPECIFICATION (See section 10, rule 13)

1. Title of the invention·. NOVEL NUCLEIC ACID PRODRUGS AND METHODS OF USE THEREOF

2. Applicant(s)

NAME

B3NTORII, INC

NATIONALITY

United States

ADDRESS

419 Western Avenue, Boston, MA 02135, United States of America

3. Preamble to the description

COMPLETE SPECIFICATION

The following specification particularly describes the invention and the manner in which it is to be performed.

PD005463IN-NP

ORIGINAL

ABSTRACT

NOVEL NUCLEIC ACID PRODRUGS AND METHODS OF USE THEREOF

Described herein are nucleic acid prodrugs and nucleic acid prodrugs comprising chiral phosphorous moieties. Also described herein are methods of making and using nucleic acid prodrugs and nucleic acid prodrugs comprising chiral phosphorous moieties.

209 [0001] This application claims the benefit of U.S. Provisional Application No. 61/223,369, filed July 6, 2009, and U.S. Provisional Application No. 61/242,722, filed September 15, 2009, each of which is incorporated herein by reference in its entirety.

FIELD OF THE INVENTION [0002] Described herein are nucleic acid prodrugs and nucleic acid prodrugs comprising chiral phosphorous moieties and methods of making and using the same.

BACKGROUND OF THE INVENTION [0003] Oligonucleotides are useful in therapeutic, diagnostic, research, and new and nanomaterials applications. The use of natural sequences of DNA or RNA is limited, for example, by their stability to nucleases. Additionally, in vitro studies have shown that the properties of antisense nucleotides such as binding affinity, sequence specific binding to the complementary RNA, stability to nucleases are affected by the configurations of the phosphorous atoms. Therefore, there is a need for prodrugs of stereodefined oligonucleotides to impart additional stability to oligonucleotide molecules in a number of in-vitro and invivo applications Prodrugs of stereodefined oligonucleotides which comprise phosphorus atom-modified nucleic acids and methods of use thereof are described herein.

SUMMARY OF THE INVENTION [0004] One embodiment provides for a chiral nucleic acid prodrug.

[0005] One embodiment provides a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0001.tif" />

R²

<img file="IN720DEN2012A_D0002.tif" />

OR³

Formula 1 wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -P(O)(R^e)2, -HP(O)(R^e), -OR* or -SR^C;

Y¹ is O, NR^d, S, or Se;

R* is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynyl· Y²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y²is O, NR^d, orS;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OR^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

at least one instance of X is -OC^CHiS-SfO^Rio, -OCH2CH2S-SCH2CH2OH, -OCH2CH2CO2H,

<img file="IN720DEN2012A_D0003.tif" />

<img file="IN720DEN2012A_D0004.tif" />

<img file="IN720DEN2012A_D0005.tif" />

<img file="IN720DEN2012A_D0006.tif" />

<img file="IN720DEN2012A_D0007.tif" />

<img file="IN720DEN2012A_D0008.tif" />

<img file="IN720DEN2012A_D0009.tif" />

<img file="IN720DEN2012A_D0010.tif" />

<img file="IN720DEN2012A_D0011.tif" />

q W .

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid;

Rio is an alkyl group having 1 to 4 carbon atoms;

Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl;

Z is S or O;

q is 0,1, or 3;

w is 1,2,3,4, 5, or 6;

Rj5 and R]₆ are independently hydrogen or methyl;

Rn is selected from alkyl, aryl or a CH₂CH=CH₂;

R₁₈ is selected from N(CH₃)₂,

<img file="IN720DEN2012A_D0012.tif" />

ΜθΟ₂0 .

n is an integer of 1 to about 200.

[0006] Another embodiment provides for a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0013.tif" />

Formula 1 wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -P(O)(R^e)2, -HP(O)(R^e), -OR* or -SR^C;

Y¹ is O, NR^d, S, or Se;

R* is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -PiOXR^, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na^+I, Li⁺¹, or K⁺¹;

Y²is O, NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OR^b ,or -SR^C, wherein R^b is a blocking group; each instance of B’ is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

<img file="IN720DEN2012A_D0014.tif" />

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid;

Rio is an alkyl group having 1 to 4 carbon atoms;

Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl; and n is an integer of 1 to about 200.

[0007] A further embodiment provides the nucleic acid prodrug wherein each X-phosphonate moiety of the compound of Formula 1 is more than 98% diastereomerically pure as determined by ^3IP NMR spectroscopy or reverse-phase HPLC.

[0008] A further embodiment provides the nucleic acid prodrug wherein each X-phosphonate moiety has a R_f configuration.

[0009] A further embodiment provides the nucleic acid prodrug wherein each X-phosphonate moiety has a SP configuration. A further embodiment provides the nucleic acid prodrug wherein each X-phosphonate independently has a RP configuration or a configuration.

[0010] A further embodiment provides the nucleic acid prodrug wherein R₁₀ is methyl. A further embodiment provides the nucleic acid prodrug wherein R_u is methyl. A further embodiment provides the nucleic acid prodrug wherein R]₂ is methyl.

[0011] A further embodiment provides the nucleic acid prodrug, wherein at least 25% of the X moieties of

R12 O /'oAAr,,

<img file="IN720DEN2012A_D0015.tif" />

the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0016.tif" />

further embodiment provides the nucleic acid prodrug, wherein at least 50% of the X moieties of the nucleic

<img file="IN720DEN2012A_D0017.tif" />

<img file="IN720DEN2012A_D0018.tif" />

<img file="IN720DEN2012A_D0019.tif" />

<img file="IN720DEN2012A_D0020.tif" />

<img file="IN720DEN2012A_D0021.tif" />

<img file="IN720DEN2012A_D0022.tif" />

<img file="IN720DEN2012A_D0023.tif" />

<img file="IN720DEN2012A_D0024.tif" />

<img file="IN720DEN2012A_D0025.tif" />

further embodiment provides the nucleic acid prodrug, wherein at least 90% of the X moieties of the nucleic acid prodrug are independently selected from

R₁₂ O /oAArh

<img file="IN720DEN2012A_D0026.tif" />

<img file="IN720DEN2012A_D0027.tif" />

<img file="IN720DEN2012A_D0028.tif" />

<img file="IN720DEN2012A_D0029.tif" />

<img file="IN720DEN2012A_D0030.tif" />

<img file="IN720DEN2012A_D0031.tif" />

<img file="IN720DEN2012A_D0032.tif" />

<img file="IN720DEN2012A_D0033.tif" />

<img file="IN720DEN2012A_D0034.tif" />

<img file="IN720DEN2012A_D0035.tif" />

further embodiment provides the nucleic acid prodrug, wherein each X moiety of the nucleic acid prodrug is independently selected from

<img file="IN720DEN2012A_D0036.tif" />

<img file="IN720DEN2012A_D0037.tif" />

V^SY%1O

<img file="IN720DEN2012A_D0038.tif" />

_Rii

<img file="IN720DEN2012A_D0039.tif" />

<img file="IN720DEN2012A_D0040.tif" />

<img file="IN720DEN2012A_D0041.tif" />

<img file="IN720DEN2012A_D0042.tif" />

<img file="IN720DEN2012A_D0043.tif" />

<img file="IN720DEN2012A_D0044.tif" />

,

<img file="IN720DEN2012A_D0045.tif" />

[0012] A further embodiment provides the nucleic acid prodrug, wherein each X moiety of the nucleic acid prodrug is independently selected from -OCH2CH₂S-S(0)2Rio, -OCH2CH2S-SCH2CH2OH, -

<img file="IN720DEN2012A_D0046.tif" />

<img file="IN720DEN2012A_D0047.tif" />

<td>^X 0'^MeMe ,</td><td> ' m^Me Me ,</td><td> (j)Me Y°'^Y^N'Me 0</td>

<td></td><td> Λ</td><td> X</td>

<td> (j)Me</td><td> (p Me</td><td> (j) Me</td>

<td> Y^s'^/V^N'^Me</td><td> Υ°--^γ^Ν'Μ_β</td><td> V^Sx/YT^N'^Me</td>

<td> 0 ,</td><td> 0 ,</td><td> 0</td>

<td>^R15,^R16 o \^ZsnS-S-R₁₇a ii</td><td>^R15^R16 r-&gt;</td><td></td>

<td>^M 0 , or</td><td> q w .</td><td></td>

<td colspan="2"> R³ is hydrogen, a blocking group, a linking moiety connected to a solid</td><td> support or a linking moiety</td>

connected to a nucleic acid;

Rio is an alkyl group having 1 to 4 carbon atoms;

Rii is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl;

Z is S or O;

q is 0,1, or 3;

w is 1, 2, 3, 4, 5, or 6;

Ris and R_i6 are independently hydrogen or methyl;

Rn is selected from alkyl, aryl or a CH₂CH=CH2; and

<img file="IN720DEN2012A_D0048.tif" />

MeO2C [0013] One embodiment provides a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0049.tif" />

wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -P(O)(R^e)2, -HP(OXR'), -OR^a or -SR‘;

Y¹ is O, NR^d, S, or Se;

R’ is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -PiOXR'X, or -HP(0)(R');

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y²is 0,NR^d, orS;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaiyl-Y¹-, -0R^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

<img file="IN720DEN2012A_D0050.tif" />

<img file="IN720DEN2012A_D0051.tif" />

<img file="IN720DEN2012A_D0052.tif" />

<img file="IN720DEN2012A_D0053.tif" />

<img file="IN720DEN2012A_D0054.tif" />

<img file="IN720DEN2012A_D0055.tif" />

wherein Rio is an alkyl group having 1 to 4 carbon atoms; Rh is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and Ri₂is hydrogen or alkyl.

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid; and n is an integer of 1 to about 200.

[0014] A further embodiment provides a nucleic acid prodrug wherein R_w is methyl. A further embodiment provides a nucleic acid prodrug wherein Rn is methyl. A further embodiment provides a nucleic acid prodrug wherein R,₂ is methyl [0015] A further embodiment provides a nucleic acid prodrug, wherein at least 25% of the X moieties of the

<img file="IN720DEN2012A_D0056.tif" />

<img file="IN720DEN2012A_D0057.tif" />

nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0058.tif" />

is an alkyl group having 1 to 4 carbon atoms; R11 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. A further embodiment provides a nucleic acid prodrug, wherein at least 50% of the X moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0059.tif" />

<img file="IN720DEN2012A_D0060.tif" />

<img file="IN720DEN2012A_D0061.tif" />

<img file="IN720DEN2012A_D0062.tif" />

<img file="IN720DEN2012A_D0063.tif" />

Y^S'-^x^T'^N'Me

O ; wherein RIO is an alkyl group having 1 to 4 carbon atoms; Rll is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. A further embodiment provides a nucleic acid prodrug, wherein at least 90% of the X moieties of the nucleic acid prodrug are independently

<img file="IN720DEN2012A_D0064.tif" />

selected from

<img file="IN720DEN2012A_D0065.tif" />

<img file="IN720DEN2012A_D0066.tif" />

<img file="IN720DEN2012A_D0067.tif" />

<img file="IN720DEN2012A_D0068.tif" />

<img file="IN720DEN2012A_D0069.tif" />

<img file="IN720DEN2012A_D0070.tif" />

to 4 carbon atoms; RI 1 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl.

A further embodiment provides a nucleic acid prodrug, wherein at each X moiety of the nucleic acid prodrug

<img file="IN720DEN2012A_D0071.tif" />

<img file="IN720DEN2012A_D0072.tif" />

is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and Ri2 is hydrogen or alkyl.

[0016] One embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the following structure:

a

<img file="IN720DEN2012A_D0073.tif" />

R² .

OR³

Formula 1 wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -P(O)(R^C)2, -HP(OXR^e), -OR* or -SR^C;

Y¹ is O, NR^d, S, or Se;

R’ is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -Ρ(Ο)(Η% or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na^+I, Li⁺ⁱ, or K⁺ⁱ;

Y²is 0,NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -0R^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

wherein at least one X moiety of the nucleic acid prodrug is independently selected from

<img file="IN720DEN2012A_D0074.tif" />

<img file="IN720DEN2012A_D0075.tif" />

<img file="IN720DEN2012A_D0076.tif" />

<img file="IN720DEN2012A_D0077.tif" />

<img file="IN720DEN2012A_D0078.tif" />

<img file="IN720DEN2012A_D0079.tif" />

<img file="IN720DEN2012A_D0080.tif" />

<img file="IN720DEN2012A_D0081.tif" />

<img file="IN720DEN2012A_D0082.tif" />

is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R]2 is hydrogen or alkyl;

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid; and n is an integer of 1 to about 200;

wherein the method used to synthesize the nucleic acid prodrug comprises the steps of: (1) reacting a molecule comprising an achiral /f-phosponate moiety and a nucleoside comprising a 5’-OH moiety to form a condensed intermediate; and (2) converting the condensed intermediate to the nucleic acid prodrug comprising a chiral X-phosphonate moiety.

[0017] Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1, wherein each X-phosphonate moiety of the compound of Formula 1 is more than 98% diastereomerically pure as determined by 3IP NMR spectroscopy or reverse-phase HPLC. Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1 wherein each X-phosphonate moiety has a RP configuration. Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1, wherein each X-phosphonate moiety has a SB configuration. Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1 wherein each X-phosphonate independently has a RP configuration or a S_P configuration.

[0018] Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1, wherein at least 25% of the X moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0083.tif" />

<img file="IN720DEN2012A_D0084.tif" />

<img file="IN720DEN2012A_D0085.tif" />

<img file="IN720DEN2012A_D0086.tif" />

Υ³^Υ^Ν^θ

Ο ; wherein RIO is an alkyl group having 1 to 4 carbon atoms; RI 1 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1, wherein at least 50% of the X moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0087.tif" />

Me

<img file="IN720DEN2012A_D0088.tif" />

ο wherein RIO is an alkyl group having 1 to 4 carbon atoms; Rll is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1, wherein at least 90% of the X moieties independently selected nucleic acid prodrug

<img file="IN720DEN2012A_D0089.tif" />

R11 ; wherein RIO is an alkyl group having 1 to 4 carbon atoms; R11 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen 16 or alkyl. Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1, wherein each instance of X is independently selected from

<img file="IN720DEN2012A_D0090.tif" />

<img file="IN720DEN2012A_D0091.tif" />

wherein Rio is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R_n is hydrogen or alkyl.

[0019] Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1 wherein R₁₀ is methyl. Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1 wherein Rn is methyl.

Another embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the structure of Formula 1 wherein R_n is methyl.

[0020] One embodiment provides a method of treating a disease associated with upregulated RNase L by administering a therapeutic amount of a chiral nucleic acid prodrug. Another embodiment provides a method of treating a disease associated with upregulated RNase L, wherein the disease is chronic fatigue syndrome. Another embodiment provides a method of treating a disease associated with downregulated RNase L by administering a therapeutic amount of a chiral nucleic acid prodrug. Another embodiment provides a method of treating a disease with downregulated RNase L, wherein the disease is cancer. IN anothoer embodiment, the cancer is selected from prostate, colorectal, and pancreatic cancer. In one embodiment, the cancer with downregulated RNase L is pancreatic cancer. In another embodiment the cancer with downregulated RNase L is prostate cancer. In yet another embodiment, the cancer with downregulated RNase L is colorectal cancer.

[0021] One embodiment provides a method of treating cancer comprising administering a therapeutic amount of a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0092.tif" />

n

Formula 1 wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -Ρ(Ο)(Κ% -ΗΡ(Ο)^°), -OR* or -SR^C;

Y¹ is O, NR^d, S, or Se;

R is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y²is O, NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N3, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OR^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

<img file="IN720DEN2012A_D0093.tif" />

at least one X moiety of the nucleic acid prodrug is independently selected from

<img file="IN720DEN2012A_D0094.tif" />

<img file="IN720DEN2012A_D0095.tif" />

Me

Me , and

<img file="IN720DEN2012A_D0096.tif" />

r” o (j) Me

Me ;wherein R₁₀ is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and Ri₂ is hydrogen or alkyl; R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid; and n is an integer of 1 to about 200;

R¹⁰ is an alkyl group having 1 to 4 carbon atoms;

wherein the method used to synthesize the nucleic acid prodrug comprises the steps of: (1) reacting a molecule comprising an achiral H-phosponate moiety and a nucleoside comprising a 5’-OH moiety to form a condensed intermediate; and (2) converting the condensed intermediate to the nucleic acid prodrug comprising a chiral X-phosphonate moiety.

[0022] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein at least 25% of the X moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0097.tif" />

<img file="IN720DEN2012A_D0098.tif" />

<img file="IN720DEN2012A_D0099.tif" />

<img file="IN720DEN2012A_D0100.tif" />

<img file="IN720DEN2012A_D0101.tif" />

<img file="IN720DEN2012A_D0102.tif" />

; wherein RIO is an alkyl group having 1 to 4 carbon atoms; Rll is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein at least 50% of the X moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0103.tif" />

<img file="IN720DEN2012A_D0104.tif" />

<img file="IN720DEN2012A_D0105.tif" />

<img file="IN720DEN2012A_D0106.tif" />

<img file="IN720DEN2012A_D0107.tif" />

<img file="IN720DEN2012A_D0108.tif" />

<img file="IN720DEN2012A_D0109.tif" />

<img file="IN720DEN2012A_D0110.tif" />

<img file="IN720DEN2012A_D0111.tif" />

<img file="IN720DEN2012A_D0112.tif" />

<img file="IN720DEN2012A_D0113.tif" />

<img file="IN720DEN2012A_D0114.tif" />

wherein RIO is an alkyl group having 1 to 4 carbon atoms; Rll is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein at least 90% of the X

R12 O moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0115.tif" />

having 1 to 4 carbon atoms; RI 1 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein each instance of X is independently selected from

<img file="IN720DEN2012A_D0116.tif" />

<img file="IN720DEN2012A_D0117.tif" />

<img file="IN720DEN2012A_D0118.tif" />

<img file="IN720DEN2012A_D0119.tif" />

<img file="IN720DEN2012A_D0120.tif" />

<img file="IN720DEN2012A_D0121.tif" />

<img file="IN720DEN2012A_D0122.tif" />

<img file="IN720DEN2012A_D0123.tif" />

<img file="IN720DEN2012A_D0124.tif" />

<img file="IN720DEN2012A_D0125.tif" />

wherein Rio is an alkyl group having 1 to 4 carbon atoms; R_n is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and Ru is hydrogen or alkyl.

[0023] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein Rio is methyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein Rn is methyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein R₁₂ is methyl.

[0024] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein each X-phosphonate moiety of the compound of Formula 1 is more than 98% diastereomerically pure as determined by ^3IP NMR spectroscopy or reverse-phase HPLC. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein each X-phosphonate moiety has a RP configuration. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein each X-phosphonate moiety has a SP configuration. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein each X-phosphonate independently has a ΛΡ configuration or a configuration.

[0025] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound of Formula 1, wherein the cancer is pancreatic cancer.

[0026] One embodiment provides a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0126.tif" />

Formula 2 wherein R¹ is -OH, -SH, -NR^dR^d, -N₃, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -P(OXR*)2, -HP(O)(R^C), -OR* or -SR^C;

Y¹ is O, NR^d, S, orSe;

R* is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y²is 0, NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -0R^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

<img file="IN720DEN2012A_D0127.tif" />

<img file="IN720DEN2012A_D0128.tif" />

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid;

Rio is an alkyl group having 1 to 4 carbon atoms;

Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl; and n is an integer of 1 to about 200.

[0027] One embodiment provides a nucleic acid prodrug of Formula 2, wherein each X-phosphonate moiety of the compound of Formula 2 is more than 98% diastereomerically pure as determined by 3IP NMR spectroscopy or reverse-phase HPLC. Another embodiment provides a nucleic acid prodrug of Formula 2, wherein each X-phosphonate moiety has a RP configuration. Another embodiment provides a nucleic acid prodrug of Formula 2, wherein each X-phosphonate moiety has a SP configuration. Another embodiment provides a nucleic acid prodrug of Formula 2, wherein each X-phosphonate independently has a ΛΡ configuration or a 5_P configuration.

[0028] Another embodiment provides a nucleic acid prodrug of Formula 2 wherein R₁₀ is methyl. Another embodiment provides a nucleic acid prodrug of Formula 2wherein Rn is methyl. Another embodiment provides a nucleic acid prodrug of Formula 2 wherein R_n is methyl [0029] Another embodiment provides a nucleic acid prodrug of Formula 2, wherein at least 25% of the X

<img file="IN720DEN2012A_D0129.tif" />

moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0130.tif" />

<img file="IN720DEN2012A_D0131.tif" />

Another embodiment provides a nucleic acid prodrug of Formula 2, wherein at least 50% of the X moieties

<img file="IN720DEN2012A_D0132.tif" />

of the nucleic acid prodrug are independently selected

<img file="IN720DEN2012A_D0133.tif" />

Another embodiment provides a nucleic acid prodrug of Formula 2, wherein at least 90% of the X moieties

<img file="IN720DEN2012A_D0134.tif" />

of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0135.tif" />

Another embodiment provides a nucleic acid prodrug of Formula 2, wherein each X moiety of the nucleic acid prodrug is independently selected from

<img file="IN720DEN2012A_D0136.tif" />

<img file="IN720DEN2012A_D0137.tif" />

<img file="IN720DEN2012A_D0138.tif" />

<img file="IN720DEN2012A_D0139.tif" />

<img file="IN720DEN2012A_D0140.tif" />

<img file="IN720DEN2012A_D0141.tif" />

[0030] One embodiment provides a pharmaceutical composition comprising a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0142.tif" />

Formula 2 wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaiyl-Y¹-, -P(O)(R^e)2, -HP(O)(R‘), -OR* or -SR^C;

Y¹ is O, NR^d, S, or Se;

R^a is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y²is O, NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OR^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

wherein at least one X moiety of the nucleic acid prodrug is independently selected from

<img file="IN720DEN2012A_D0143.tif" />

<img file="IN720DEN2012A_D0144.tif" />

<img file="IN720DEN2012A_D0145.tif" />

<img file="IN720DEN2012A_D0146.tif" />

<img file="IN720DEN2012A_D0147.tif" />

<img file="IN720DEN2012A_D0148.tif" />

is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R₁₂ is hydrogen or alkyl;

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid; and n is an integer of 1 to about 200;

wherein the method used to synthesize the nucleic acid prodrug comprises the steps of: (1) reacting a molecule comprising an achiral 77-phosponate moiety and a nucleoside comprising a 5’-OH moiety to form a condensed intermediate; and (2) converting the condensed intermediate to the nucleic acid prodrug comprising a chiral X-phosphonate moiety.

[0031] Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2 wherein each X-phosphonate moiety of the compound of Formula 2 is more than 98% diastereomerically pure as determined by 3 IP NMR spectroscopy or reverse-phase HPLC. Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2 wherein each X-phosphonate moiety has a RP configuration. Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2 wherein each X-phosphonate moiety has a SP configuration. Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2 wherein each X-phosphonate independently has a RP configuration or a S_P configuration.

[0032] Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2, wherein at least 25% of the X moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0149.tif" />

wherein RIO is an alkyl group having 1 to 4 carbon atoms; Rll is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2, wherein at least 50% of the X moieties of the nucleic acid prodrug are

<img file="IN720DEN2012A_D0150.tif" />

independently selected from

<img file="IN720DEN2012A_D0151.tif" />

<img file="IN720DEN2012A_D0152.tif" />

<img file="IN720DEN2012A_D0153.tif" />

<img file="IN720DEN2012A_D0154.tif" />

<img file="IN720DEN2012A_D0155.tif" />

<img file="IN720DEN2012A_D0156.tif" />

<img file="IN720DEN2012A_D0157.tif" />

<img file="IN720DEN2012A_D0158.tif" />

<img file="IN720DEN2012A_D0159.tif" />

<img file="IN720DEN2012A_D0160.tif" />

<img file="IN720DEN2012A_D0161.tif" />

<img file="IN720DEN2012A_D0162.tif" />

Ο ; wherein RIO is an alkyl group having 1 to 4 carbon atoms; R11 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2, wherein at least 90% of the X moieties of

<img file="IN720DEN2012A_D0163.tif" />

<img file="IN720DEN2012A_D0164.tif" />

the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0165.tif" />

<img file="IN720DEN2012A_D0166.tif" />

<img file="IN720DEN2012A_D0167.tif" />

; wherein RIO is an alkyl group having 1 to 4 carbon atoms; RI 1 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a pharmaceutical composition comprising a compound of Formula

2,

R12 O /sAAr„ wherein each instance of X is independently selected from

<img file="IN720DEN2012A_D0168.tif" />

wherein R_to is an alkyl group having 1 to 4 carbon atoms; Ru is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R_[2 is hydrogen or alkyl.

[0033] Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2 wherein Rio is methyl. Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2 wherein Rn is methyl. Another embodiment provides a pharmaceutical composition comprising a compound of Formula 2 wherein R₁₂ is methyl.

[0034] One embodiment provides a method of treating cancer comprising administering a therapeutic amount of a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0169.tif" />

wherein R¹ is -OH, -SH, -NR^dR^d, -N₃, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -PiOXR'fc, -HPiOXR⁰), -OR* or -SR^C;

Y¹ is O, NR^d, S, or Se;

R^a is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(0)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y²is 0,NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -0R^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

<img file="IN720DEN2012A_D0170.tif" />

at least one X moiety of the nucleic acid prodrug is independently selected from

<img file="IN720DEN2012A_D0171.tif" />

<img file="IN720DEN2012A_D0172.tif" />

to 4 carbon atoms; R_H is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R_u is hydrogen or alkyl; R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid; and n is an integer of 1 to about 200;

R¹⁰ is an alkyl group having 1 to 4 carbon atoms;

wherein the method used to synthesize the nucleic acid prodrug comprises the steps of: (1) reacting a molecule comprising an achiral /7-phosponate moiety and a nucleoside comprising a 5’-OH moiety to form a condensed intermediate; and (2) converting the condensed intermediate to the nucleic acid prodrug comprising a chiral X-phosphonate moiety.

[0035] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein at least 25% of the X moieties of the

<img file="IN720DEN2012A_D0173.tif" />

<img file="IN720DEN2012A_D0174.tif" />

nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0175.tif" />

<img file="IN720DEN2012A_D0176.tif" />

<img file="IN720DEN2012A_D0177.tif" />

<img file="IN720DEN2012A_D0178.tif" />

<img file="IN720DEN2012A_D0179.tif" />

<img file="IN720DEN2012A_D0180.tif" />

<img file="IN720DEN2012A_D0181.tif" />

<img file="IN720DEN2012A_D0182.tif" />

<img file="IN720DEN2012A_D0183.tif" />

<img file="IN720DEN2012A_D0184.tif" />

; wherein RIO is an alkyl group having 1 to 4 carbon atoms; R11 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein at least 50% of the X moieties of the nucleic acid prodrug are independently selected from

<img file="IN720DEN2012A_D0185.tif" />

<img file="IN720DEN2012A_D0186.tif" />

<img file="IN720DEN2012A_D0187.tif" />

<img file="IN720DEN2012A_D0188.tif" />

<img file="IN720DEN2012A_D0189.tif" />

having 1 to 4 carbon atoms; R11 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein at least 90% of the X moieties of the nucleic acid prodrug are independently selected from

R12 O /oAArh

<img file="IN720DEN2012A_D0190.tif" />

<img file="IN720DEN2012A_D0191.tif" />

<img file="IN720DEN2012A_D0192.tif" />

<td></td><td></td><td> \/0^^©.Me ' T^xMe</td>

<td> 0</td><td> 0</td><td> Me^Me ,</td>

<td></td><td> (j)Me</td><td> (j)Me</td>

<td> X/S-^x^^Me ^X Y'Me</td><td> \^Ό'^γ^Ν'Μβ</td><td></td>

<td> Me ,</td><td> 0</td><td> o</td>

<td> Λ</td><td></td><td></td>

<td> (j) Me</td><td> ψ Me</td><td></td>

<td> V°^Y^N'^Me</td><td></td><td></td>

<td> 0</td><td> and 0</td><td> ;wherein RIO is an alkyl group</td>

having 1 to 4 carbon atoms; R11 is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R12 is hydrogen or alkyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein each instance of X is independently

<img file="IN720DEN2012A_D0193.tif" />

<img file="IN720DEN2012A_D0194.tif" />

<img file="IN720DEN2012A_D0195.tif" />

<img file="IN720DEN2012A_D0196.tif" />

<img file="IN720DEN2012A_D0197.tif" />

<img file="IN720DEN2012A_D0198.tif" />

and

<img file="IN720DEN2012A_D0199.tif" />

Y^S'^Y^N'Me

Ο ; wherein Ri₀ is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R_n is hydrogen or alkyl.

[0036] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein Rio is methyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein Rn is methyl. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein Ri₂ is methyl [0037] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein each X-phosphonate moiety of the compound of Formula 2 is more than 98% diastereomerically pure as determined by ³¹P NMR spectroscopy or reverse-phase HPLC.

[0038] Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein each X-phosphonate moiety has a RP configuration. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein each X-phosphonate moiety has a SP configuration. Another embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein each X-phosphonate independently has a RP configuration or a S? configuration.

[0039] One embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein the cancer is pancreatic cancer.

[0040] One embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein the compound has the following formula:

<img file="IN720DEN2012A_D0200.tif" />

Formula A₃-2 wherein each A is adenine and each R] i is independently selected from alkyl, aryl, heteroaryl, heterocyclyl, and cycloalkyl. A further embodiment provides a method of treating pancreatic cancer comprising administering a therapeutic amount of a compound of Formula A₃-2.

[0041] One embodiment provides a method of treating cancer comprising administering a therapeutic amount of a compound having the structure of Formula 2, wherein the compound has the following formula:

<img file="IN720DEN2012A_D0201.tif" />

[0042] A further embodiment provides a method of treating pancreatic cancer comprising administering a therapeutic amount of a compound of Formula A₃-3.

[0043] One embodiment provides a compound or its pharmaceutically acceptable salt having the following formula:

<img file="IN720DEN2012A_D0202.tif" />

HO OH

Formula A₃-l

<img file="IN720DEN2012A_D0203.tif" />

<img file="IN720DEN2012A_D0204.tif" />

wherein each A is adenine; and at least one X moiety is

<img file="IN720DEN2012A_D0205.tif" />

to 4 carbon atoms; Rh is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and Ru is hydrogen or alkyl.

[0044] Another embodiment provides a compound or its pharmaceutically acceptable salt having the structure of Formula A3-1, wherein at least two of the X moieties of the nucleic acid prodrug are

<img file="IN720DEN2012A_D0206.tif" />

independently selected from

<img file="IN720DEN2012A_D0207.tif" />

; wherein R_w is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and R_]2 is hydrogen or alkyl.

[0045] Another embodiment provides a compound or its pharmaceutically acceptable salt having the structure of Formula A₃-l, wherein at least three of the X moieties of the nucleic acid prodrug are

R₁₂ θ Rn O /

AAAr,, /'•sAAr,, ⁰

9

Y^R independently selected from

<img file="IN720DEN2012A_D0208.tif" />

<img file="IN720DEN2012A_D0209.tif" />

<img file="IN720DEN2012A_D0210.tif" />

<img file="IN720DEN2012A_D0211.tif" />

<img file="IN720DEN2012A_D0212.tif" />

<img file="IN720DEN2012A_D0213.tif" />

<img file="IN720DEN2012A_D0214.tif" />

<img file="IN720DEN2012A_D0215.tif" />

<img file="IN720DEN2012A_D0216.tif" />

; wherein Ri₀ is an alkyl group having 1 to 4 carbon atoms; Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl; and Ri₂ is hydrogen or alkyl.

[0046] Another embodiment provides a compound or its pharmaceutically acceptable salt having the structure of Formula A₃-l, wherein each X moiety of the nucleic acid prodrug is independently selected from

<img file="IN720DEN2012A_D0217.tif" />

<img file="IN720DEN2012A_D0218.tif" />

cycloalkyl; and Ru is hydrogen or alkyl.

[0047] Another embodiment provides a compound or its pharmaceutically acceptable salt having the structure of Formula A₃-l, wherein the compound has the following formula:

<img file="IN720DEN2012A_D0219.tif" />

INCORPORATION BY REFERENCE [0048] All publications and patent applications disclosed herein in this specification are herein incorporated by reference in their entirety to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

BRIEF DESCRIPTION OF THE DRAWINGS [0049] The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings.

[0050] Figure 1 provides a representative analytical HPLC profile for compound 64 and GSH.

[0051] Figure 2 provides provided a representative HPLC profile of compound 64a, a glutathione adduct, and the final product after release from the pro-moeity.

[0052] Figure 3 provides a plot of conversion over time for compound 64a and 64b.

[0053] Figure 4 provides a reaction timecourse as determined by LC-MS for the glutathione assisted prodrug release of compound 64a.

DETAILED DESCRIPTION OF THE INVENTION [0054] The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described. All documents, or portions of documents, cited in the application including, without limitation, patents, patent applications, articles, books, manuals, and treatises are hereby expressly incorporated by reference in their entirety for any purpose.

[0055] Unless otherwise stated, the following terms used in this application, including the specification and claims, have the definitions given below. It must be noted that, as used in the specification and the appended claims, the singular forms “a” “an” and “the” include plural referents unless the context clearly dictates otherwise. Unless otherwise indicated, conventional methods of mass spectroscopy, NMR, HPLC, protein chemistry, biochemistry, recombinant DNA techniques and pharmacology are employed. In this application, the use of “or” or “and” means “and/or” unless stated otherwise. Furthermore, use of the term “including” as well as other forms, such as “include”, “includes” and “included” is not limiting.

Certain Chemical Terminology [0056] Unless otherwise noted, the use of general chemical terms, such as though not limited to alkyl, amine, aryl, are unsubstituted.

[0057] As used herein, C_rC_x includes C_rC₂,C]-C₃... C_rC_x. By way of example only, a group designated as C1-C4 indicates that there are one to four carbon atoms in the moiety, i.e. groups containing 1 carbon atom, 2 carbon atoms, 3 carbon atoms or 4 carbon atoms, as well as the ranges C_rC₂ and C_rC₃. Thus, by way of example only, C_rC₄ alkyl indicates that there are one to four carbon atoms in the alkyl group, i.e., the alkyl group is selected from among methyl, ethyl, propyl, iso-propyl, n-biityl, iso-butyl, sec-butyl, and tbutyl. Whenever it appears herein, a numerical range such as 1 to 10 refers to each integer in the given range; e.g., 1 to 10 carbon atoms means that the group may have 1 carbon atom, 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms, 6 carbon atoms, 7 carbon atoms, 8 carbon atoms, 9 carbon atoms, or 10 carbon atoms.

[0058] The terms heteroatom or hetero as used herein, alone or in combination, refer to an atom other than carbon or hydrogen. Heteroatoms are may be independently selected from among oxygen, nitrogen, sulfur, phosphorous, silicon, selenium and tin but are not limited to these atoms. In embodiments in which two or more heteroatoms are present, the two or more heteroatoms can be the same as each another, or some or all of the two or more heteroatoms can each be different from the others.

[0059] The term alkyl as used herein, alone or in combination, refers to a straight-chain or branched-chain saturated hydrocarbon monoradical having from one to about ten carbon atoms, or one to six carbon atoms. Examples include, but are not limited to methyl, ethyl, n-propyl, isopropyl, 2-methyl-l-propyl, 2-methyl-2propyl, 2-methyl-l-butyl, 3-methyl-1-butyl, 2-methyl-3-butyl, 2,2-dimethyl-l-propyl, 2-methyl-l-pentyl, 3methyl-1-pentyl, 4-methyl-l-pentyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl, 4-methyl-2-pentyl, 2,2-dimethyl1-butyl, 3,3-dimethyl-1 -butyl, 2-ethyl-l-butyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopentyl, 42 neopentyl, tert-amyl and hexyl, and longer alkyl groups, such as heptyl, octyl and the like. Whenever it appears herein, a numerical range such as C_rC₆ alkyl or C^ alkyl, means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms. In one embodiment, the “alkyl” is substituted. Unless otherwise indicated, the “alkyl” is unsubstititued.

[0060] The term alkenyl as used herein, alone or in combination, refers to a straight-chain or branchedchain hydrocarbon monoradical having one or more carbon-carbon double-bonds and having from two to about ten carbon atoms, or two to about six carbon atoms. The group may be in either the cis or trans conformation about the double bond(s), and should be understood to include both isomers. Examples include, but are not limited to ethenyl (-CH=CH₂), 1-propenyl (-CH₂CH=CH₂), isopropenyl [-C(CH₃)=CH₂], butenyl, 1,3-butadienyl and the like. Whenever it appears herein, a numerical range such as C₂-C₆ alkenyl or C₂_6 alkenyl, means that the alkenyl group may consist of 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms. In one embodiment, the “alkenyl” is substituted. Unless otherwise indicated, the “alkenyl” is unsubstititued.

[0061] The term alkynyl as used herein, alone or in combination, refers to a straight-chain or branchedchain hydrocarbon monoradical having one or more carbon-carbon triple-bonds and having from two to about ten carbon atoms, or from two to about six carbon atoms. Examples include, but are not limited to ethynyl, 2-propynyl, 2-butynyl, 1,3-butadiynyl and the like. Whenever it appears herein, a numerical range such as C₂-C₆ alkynyl or C₂.₆ alkynyl, means that the alkynyl group may consist of 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms. In one embodiment, the “alkynyl” is substituted. Unless otherwise indicated, the “alkynyl” is unsubstititued.

[0062] The terms heteroalkyl, heteroalkenyl and heteroalkynyl as used herein, alone or in combination, refer to alkyl, alkenyl and alkynyl structures respectively, as described above, in which one or more of the skeletal chain carbon atoms (and any associated hydrogen atoms, as appropriate) are each independently replaced with a heteroatom (i.e. an atom other than carbon, such as though not limited to oxygen, nitrogen, sulfur, silicon, phosphorous, tin or combinations thereof), or heteroatomic group such as though not limited to -O-O-, -S-S-, -Ο-S-, -S-Ο-, =N-N=, -Ν=Ν-, -Ν=Ν-ΝΗ-, -Ρ(Ο)₂-, -O-P(O)₂-, -P(O)₂-O, -S(O)-, -S(O)₂-, -SnH₂- and the like.

[0063] The terms haloalkyl, haloalkenyl and haloalkynyl as used herein, alone or in combination, refer to alkyl, alkenyl and alkynyl groups respectively, as defined above, in which one or more hydrogen atoms is replaced by fluorine, chlorine, bromine or iodine atoms, or combinations thereof. In some embodiments two or more hydrogen atoms may be replaced with halogen atoms that are the same as each another (e.g. difluoromethyl); in other embodiments two or more hydrogen atoms may be replaced with halogen atoms that are not all the same as each other (e.g. 1-chloro-1-fluoro-1-iodoethyl). Non-limiting examples of haloalkyl groups are fluoromethyl, chloromethyl and bromoethyl. A non-limiting example of a haloalkenyl group is bromoethenyl. A non-limiting example of a haloalkynyl group is chloroethynyl.

[0064] The term carbon chain as used herein, alone or in combination, refers to any alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl or heteroalkynyl group, which is linear, cyclic, or any combination thereof. If the chain is part of a linker and that linker comprises one or more rings as part of the core backbone, for purposes of calculating chain length, the chain only includes those carbon atoms that compose the bottom or top of a given ring and not both, and where the top and bottom of the ring(s) are not equivalent in length, the shorter distance shall be used in determining the chain length. If the chain contains heteroatoms as part of the backbone, those atoms are not calculated as part of the carbon chain length.

[0065] The term cycloalkyl as used herein, alone or in combination, refers to a saturated, hydrocarbon monoradical ring, containing from three to about fifteen ring carbon atoms or from three to about ten ring carbon atoms, though may include additional, non-ring carbon atoms as substituents (e.g. methylcyclopropyl). Whenever it appears herein, a numerical range such as C₃-C₆ cycloalkyl or C_Mcycloalkyl, means that the cycloalkyl group may consist of 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms, i.e., is cyclopropyl, cyclobutyl, cyclopentyl or cyclohepty, although the present definition also covers the occurrence of the term cycloalkyl where no numerical range is designated. The term includes fused, non-fused, bridged and spiro radicals. A fused cycloalkyl may contain from two to four fused rings where the ring of attachment is a cycloalkyl ring, and the other individual rings may be alicyclic, heterocyclic, aromatic, heteroaromatic or any combination thereof. Examples include, but are not limited to cyclopropyl, cyclopentyl, cyclohexyl, decalinyl, and bicyclo [2.2.1] heptyl and adamantyl ring systems. Illustrative examples include, but are not limited to the following moieties:

and the like.

In one embodiment, the “cycloalkyl” is substituted. Unless otherwise indicated, the “cycloalkyl” is unsubstititued.

[0066] The terms non-aromatic heterocyclyl and heteroalicyclyl as used herein, alone or in combination, refer to a saturated, partially unsaturated, or fully unsaturated nonaromatic ring monoradicals containing from three to about twenty ring atoms, where one or more of the ring atoms are an atom other than carbon, independently selected from among oxygen, nitrogen, sulfur, phosphorous, silicon, selenium and tin but are not limited to these atoms. In embodiments in which two or more heteroatoms are present in the ring, the two or more heteroatoms can be the same as each another, or some or all of the two or more heteroatoms can each be different from the others. The terms include fused, non-fused, bridged and spiro radicals. A fused non-aromatic heterocyclic radical may contain from two to four fused rings where the attaching ring is a nonaromatic heterocycle, and the other individual rings may be alicyclic, heterocyclic, aromatic, heteroaromatic or any combination thereof. Fused ring systems may be fused across a single bond or a double bond, as well as across bonds that are carbon-carbon, carbon-hetero atom or hetero atom-hetero atom. The terms also include radicals having from three to about twelve skeletal ring atoms, as well as those having from three to about ten skeletal ring atoms. Attachment of a non-aromatic heterocyclic subunit to its parent molecule can be via a heteroatom or a carbon atom. Likewise, additional substitution can be via a heteroatom or a carbon atom. As a non-limiting example, an imidazolidine non-aromatic heterocycle may be attached to a parent molecule via either of its N atoms (imidazolidin-l-yl or imidazolidin-3-yl) or any of its carbon atoms (imidazolidin-2-yl, imidazolidin-4-yl or imidazolidin-5-yl). In certain embodiments, non-aromatic heterocycles contain one or more carbonyl or thiocarbonyl groups such as, for example, oxo- and thiocontaining groups. Examples include, but are not limited to pyrrolidinyl, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, dihydropyranyl, tetrahydrothiopyranyl, piperidino, morpholino, thiomorpholino, thioxanyl, piperazinyl, azetidinyl, oxetanyl, thietanyl, homopiperidinyl, oxepanyl, thiepanyl, oxazepinyl, diazepinyl, thiazepinyl, 1,2,3,6-tetrahydropyridinyl, 2-pyrrolinyl, 3-pyrrolinyl, indolinyl, 2Hpyranyl, 4H-pyranyl, dioxanyl, 1,3-dioxolanyl, pyrazolinyl, dithianyl, dithiolanyl, dihydropyranyl, dihydrothienyl, dihydrofuranyl, pyrazolidinyl, imidazolinyl, imidazolidinyl, 3-azabicyclo[3.1.0]hexanyl, 3azabicyclo[4.1.0]heptanyl, 3H-indolyl and quinolizinyl. Illustrative examples of heterocycloalkyl groups, also refened to as non-aromatic heterocycles, include:

O . ό · 0 · 0 OO zb

A , A ,

NH HN-NH

O^O \__/ n

Q 0 db

NH

A A °7^S'\ ’ HN^JNH ’ ’ \^J ’

<img file="IN720DEN2012A_D0220.tif" />

and the like.

The terms also include all ring forms of the carbohydrates, including but not and the oligosaccharides. In one embodiment, the is substituted. Unless otherwise indicated, the limited to the “non-aromatic “non-aromatic [0067] monosaccharides, the disaccharides heterocyclyl” or “heteroalicyclyl” heterocyclyl” or “heteroalicyclyl” is unsubstititued.

[0068] The term aryl as used herein, alone or in combination, refers to an aromatic hydrocarbon radical of six to about twenty ring carbon atoms, and includes fused and non-fused aryl rings. A fused aryl ring radical contains from two to four fused rings where the ring of attachment is an aryl ring, and the other individual rings may be alicyclic, heterocyclic, aromatic, heteroaromatic or any combination thereof. Further, the term aryl includes fused and non-fused rings containing from six to about twelve ring carbon atoms, as well as those containing from six to about ten ring carbon atoms. A non-limiting example of a single ring aryl group includes phenyl; a fused ring aryl group includes naphthyl, phenanthrenyl, anthracenyl, azulenyl; and a non45 fused bi-aryl group includes biphenyl. In one embodiment, the “aryl” is substituted. Unless otherwise indicated, the “aryl” is unsubstititued.

[0069] The term heteroaryl as used herein, alone or in combination, refers to an aromatic monoradicals containing from about five to about twenty skeletal ring atoms, where one or more of the ring atoms is a heteroatom independently selected from among oxygen, nitrogen, sulfur, phosphorous, silicon, selenium and tin but not limited to these atoms and with the proviso that the ring of said group does not contain two adjacent O or S atoms. In embodiments in which two or more heteroatoms are present in the ring, the two or more heteroatoms can be the same as each another, or some or all of the two or more heteroatoms can each be different from the others. The term heteroaryl includes fused and non-fused heteroaryl radicals having at least one heteroatom. The term heteroaryl also includes fused and non-fused heteroaryls having from five to about twelve skeletal ring atoms, as well as those having from five to about ten skeletal ring atoms. Bonding to a heteroaryl group can be via a carbon atom or a heteroatom. Thus, as a non-limiting example, an imidazole group may be attached to a parent molecule via any of its carbon atoms (imidazol-2-yl, imidazol4-yl or imidazol-5-yl), or its nitrogen atoms (imidazol-l-yl or imidazol-3-yl). Likewise, a heteroaryl group may be further substituted via any or all of its carbon atoms, and/or any or all of its heteroatoms. A fused heteroaryl radical may contain from two to four fused rings where the ring of attachment is a heteroaromatic ring and the other individual rings may be alicyclic, heterocyclic, aromatic, heteroaromatic or any combination thereof. A non-limiting example of a single ring heteroaryl group includes pyridyl; fused ring heteroaryl groups include benzimidazolyl, quinolinyl, acridinyl; and a non-fused bi-heteroaryl group includes bipyridinyl. Further examples of heteroaryls include, without limitation, furanyl, thienyl, oxazolyl, acridinyl, phenazinyl, benzimidazolyl, benzofuranyl, benzoxazolyl, benzothiazolyl, benzothiadiazolyl, benzothiophenyl, benzoxadiazolyl, benzotriazolyl, imidazolyl, indolyl, isoxazolyl, isoquinolinyl, indolizinyl, isothiazolyl, isoindolyloxadiazolyl, indazolyl, pyridyl, pyridazyl, pyrimidyl, pyrazinyl, pyrrolyl, pyrazinyl, pyrazolyl, purinyl, phthalazinyl, pteridinyl, quinolinyl, quinazolinyl, quinoxalinyl, triazolyl, tetrazolyl, thiazolyl, triazinyl, thiadiazolyl and the like, and their oxides, such as for example pyridyl-N-oxide. Illustrative examples of heteroaryl groups include the following moieties:

<img file="IN720DEN2012A_D0221.tif" />

<img file="IN720DEN2012A_D0222.tif" />

In one embodiment, the “heteroaryl” is substituted. Unless otherwise indicated, the “heteroaryl” is unsubstititued.

[0070J The term heterocyclyl as used herein, alone or in combination, refers collectively to heteroalicyclyl and heteroaryl groups. Herein, whenever the number of carbon atoms in a heterocycle is indicated (e.g., C_r

C₆ heterocycle), at least one non-carbon atom (the heteroatom) must be present in the ring. Designations such as Ci-C₆ heterocycle” refer only to the number of carbon atoms in the ring and do not refer to the total number of atoms in the ring. Designations such as 4-6 membered heterocycle refer to the total number of atoms that are contained in the ring (i.e., a four, five, or six membered ring, in which at least one atom is a carbon atom, at least one atom is a heteroatom and the remaining two to four atoms are either carbon atoms or heteroatoms). For heterocycles having two or more heteroatoms, those two or more heteroatoms can be the same or different from one another. Non-aromatic heterocyclic groups include groups having only three atoms in the ring, while aromatic heterocyclic groups must have at least five atoms in the ring. Bonding (i.e. attachment to a parent molecule or further substitution) to a heterocycle can be via a heteroatom or a carbon atom. In one embodiment, the “heterocyclyl” is substituted. Unless otherwise indicated, the “heterocycyl” is unsubstititued.

[0071] The terms halogen, halo or halide as used herein, alone or in combination refer to fluoro, chloro, bromo and/or iodo.

Certain Pharmaceutical Terminology [0072] The term subject, patient or individual as used herein in reference to individuals suffering from a disorder, and the like, encompasses mammals and non-mammals. Examples of mammals include, but are not limited to, any member of the Mammalian class: humans, non-human primates such as chimpanzees, and other apes and monkey species; farm animals such as cattle, horses, sheep, goats, swine; domestic animals such as rabbits, dogs, and cats; laboratory animals including rodents, such as rats, mice and guinea pigs, and the like. Examples of non-mammals include, but are not limited to, birds, fish and the like. In one embodiment of the methods and compositions provided herein, the mammal is a human.

[0073] The terms effective amount, therapeutically effective amount or pharmaceutically effective amount as used herein, refer to an amount of at least one agent or compound being administered that is sufficient to treat or prevent the particular disease or condition. The result can be reduction and/or alleviation of the signs, symptoms, or causes of a disease, or any other desired alteration of a biological system. For example, an effective amount for therapeutic uses is the amount of the composition comprising a compound as disclosed herein required to provide a clinically significant decrease in a disease. An appropriate effective amount in any individual case may be determined using techniques, such as a dose escalation study.

[0074] As used herein, “treatment” or “treating,” or “palliating” or “ameliorating” are used interchangeably herein. These terms refers to an approach for obtaining beneficial or desired results including but not limited to therapeutic benefit and/or a prophylactic benefit. By therapeutic benefit is meant eradication or amelioration of the underlying disorder being treated. Also, a therapeutic benefit is achieved with the eradication or amelioration of one or more of the physiological symptoms associated with the underlying disorder such that an improvement is observed in the patient, notwithstanding that the patient may still be afflicted with the underlying disorder. For prophylactic benefit, the compositions may be administered to a patient at risk of developing a particular disease, or to a patient reporting one or more of the physiological symptoms of a disease, even though a diagnosis of this disease may not have been made.

[0075] A “therapeutic effect,” as that term is used herein, encompasses a therapeutic benefit and/or a prophylactic benefit as described above. A prophylactic effect includes delaying or eliminating the appearance of a disease or condition, delaying or eliminating the onset of symptoms of a disease or condition, slowing, halting, or reversing the progression of a disease or condition, or any combination thereof.

[0076] The term pharmaceutically acceptable as used herein, refers to a material, such as a carrier or diluent, which does not abrogate the biological activity or properties of the compounds described herein, and is relatively nontoxic, i.e., the material may be administered to an individual without causing undesirable biological effects or interacting in a deleterious manner with any of the components of the composition in which it is contained.

[0077] The term pharmaceutical composition, as used herein, refers to a biologically active compound, optionally mixed with at least one pharmaceutically acceptable chemical component, such as, though not limited to carriers, stabilizers, diluents, dispersing agents, suspending agents, thickening agents, and/or excipients.

[0078] The term carrier as used herein, refers to relatively nontoxic chemical compounds or agents that facilitate the incorporation of a compound into cells or tissues.

[0079] The term “prodrug” is meant to indicate a compound that may be converted under physiological conditions or by solvolysis to a biologically active compound described herein. Thus, the term prodrug refers to a precursor of a biologically active compound that is pharmaceutically acceptable. A prodrug may be inactive when administered to a subject, but is converted in vivo to an active compound, for example, by hydrolysis. The prodrug compound often offers advantages of solubility, tissue compatibility or delayed release in a mammalian organism (see, e.g., Bundgard, H., Design of Prodrugs (1985), pp. 7-9, 21-24 (Elsevier, Amsterdam). A discussion of prodrugs is provided in Higuchi, T., et al., Pro-drugs as Novel Delivery Systems, A.C.S. Symposium Series, Vol. 14, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated in full by reference herein. The term “prodrug” is also meant to include any covalently bonded carriers, which release the active compound in vivo when such prodrug is administered to a mammalian subject. Prodrugs of an active compound, as described herein, may be prepared by modifying functional groups present in the active compound in such a way that the modifications are cleaved, either in routine manipulation or in vivo, to the parent active compound. Prodrugs include compounds wherein a hydroxy, amino or mercapto group is bonded to any group that, when the prodrug of the active compound is administered to a mammalian subject, cleaves to form a free hydroxy, free amino or free mercapto group, respectively. Examples of prodrugs include, but are not limited to acyloxy, thioacyloxy, 2carboalkoxyethyl, disulfide, thiaminal, and enol ester derivatives of a phosphorus atom-modified nucleic acid.

[0080] The term “pro-oligonucleotide” or “pronucleotide” or “nucleic acid prodrug” refers to an oligonucleotide which has been modified to be a prodrug of the oligonucleotide.

Certain Nucleic Acid Terminology [0081] Natural nucleic acids have a phosphate backbone; artificial nucleic acids may contain other types of backbones, but contain the same bases.

[0082] The term “nucleotide” as used herein refers to a monomeric unit of a polynucleotide that consists of a heterocyclic base, a sugar, and one or more phosphate groups. The naturally occurring bases, (guanine, (G), adenine, (A), cytosine, (C), thymine, (T), and uracil (U)) are derivatives of purine or pyrimidine, though it should be understood that naturally and non-naturally occurring base analogs are also included. The naturally occurring sugar is the pentose (five-carbon sugar) deoxyribose (which forms DNA) or ribose (which forms RNA), though it should be understood that naturally and non-naturally occurring sugar analogs are also included. Nucleic acids are linked via phosphate bonds to form nucleic acids, or polynucleotides, though many other linkages are known in the art (such as, though not limited to phosphorothioates, boranophosphates and the like). Artificial nucleic acids include PNAs (peptide nucleic acids), phosphothionates, and other variants of the phosphate backbone of native nucleic acids.

[0083] The term “nucleoside” refers to a moiety wherein a nucleobase or a modified nucleobase is covalently bound to a sugar or modified sugar.

[0084] The term “sugar” refers to a monosaccharide in closed and/or open form. Sugars include, but are not limited to, ribose, deoxyribose, pentofuranose, pentopyranose, and hexopyranose moieties.

[0085] The term “modified sugar” refers to a moiety that can replace a sugar. The modified sugar mimics the spatial arrangement, electronic properties, or some other physicochemical property of a sugar.

[0086] The terms “nucleic acid” and “polynucleotide” as used herein refer to a polymeric form of nucleotides of any length, either ribonucleotides (RNA) or deoxyribonucleotides (DNA). These terms refer to the primary structure of the molecules and, thus, include double- and single-stranded DNA, and doubleand single-stranded RNA. These terms include, as equivalents, analogs of either RNA or DNA made from nucleotide analogs and modified polynucleotides such as, though not limited to, methylated and/or capped polynucleotides. The terms encompass poly- or oligo-ribonucleotides (RNA) and poly- or oligodeoxyribonucleotides (DNA); RNA or DNA derived from N-glycosides or C-glycosides of nucleobases and/or modified nucleobases; nucleic acids derived from sugars and/or modified sugars; and nucleic acids derived from phosphate bridges and/or modified phosphorous-atom bridges. The term encompasses nucleic acids containing any combinations of nucleobases, modified nucleobases, sugars, modified sugars, phosphate bridges or modified phosphorous atom bridges. Examples include, and are not limited to, nucleic acids containing ribose moieties, the nucleic acids containing deoxy-ribose moieties, nucleic acids containing both ribose and deoxyribose moieties, nucleic acids containing ribose and modified ribose moieties. The prefix poly- refers to a nucleic acid containing about 1 to about 10,000 nucleotide monomer units and wherein the prefix oligo- refers to a nucleic acid containing about 1 to about 200 nucleotide monomer units.

[0087] The term “nucleobase” refers to the parts of nucleic acids that are involved in the hydrogen-bonding that binds one nucleic acid strand to another complementary strand in a sequence specific manner. The most common naturally-occurring nucleobases are adenine (A), guanine (G), uracil (U), cytosine (C), and thymine (T).

[0088] The term “modified nucleobase” refers to a moiety that can replace a nucleobase. The modified nucleobase mimics the spatial arrangement, electronic properties, or some other physicochemical property of the nucleobase and retains the property of hydrogen-bonding that binds one nucleic acid strand to another in a sequence specific manner. A modified nucleobase can pair with all of the five naturally occurring bases (uracil, thymine, adenine, cytosine, or guanine) without substantially affecting the melting behavior, recognition by intracellular enzymes or activity of the oligonucleotide duplex.

[0089] The term “chiral reagent” refers to a compound that is chiral or enantiopure and can be used for asymmetric induction in nucleic acid synthesis.

[0090] The term “chiral ligand” or “chiral auxiliary” refers to a moiety that is chiral or enantiopure and controls the stereochemical outcome of a reaction.

[0091] In a condensation reaction, the term “condensing reagent” refers to a reagent that activates a less reactive site and renders it more susceptible to attack by a nucleophile.

[0092] The term “blocking group” refers to a group that transiently masks the reactivity of a functional group. The functional group can be subsequently unmasked by removal of the blocking group.

[0093] The terms “boronating agents”, “sulfur electrophiles”, “selenium electrophiles” refer to compounds that are useful in the modifying step used to introduce BH₃, S, and Se groups, respectively, for modification at the phosphorus atom.

[0094] The term “moiety” refers to a specific segment or functional group of a molecule. Chemical moieties are often recognized chemical entities embedded in or appended to a molecule.

[0095] The term “solid support” refers to any support which enables synthetic mass production of nucleic acids and can be reutilized at need. As used herein, the term refers to a polymer, that is insoluble in the media employed in the reaction steps performed to synthesize nucleic acids, and is derivatized to comprise reactive groups.

[0096] The term “linking moiety” refers to any moiety optionally positioned between the terminal nucleoside and the solid support or between the terminal nucleoside and another nucleoside, nucleotide, or nucleic acid.

(0097] A “DNA molecule” refers to the polymeric form of deoxyribonucleotides (adenine, guanine, thymine, or cytosine) in its either single stranded form or a double-stranded helix. This term refers only to the primary and secondary structure of the molecule, and does not limit it to any particular tertiary forms. Thus, this term includes double-stranded DNA found, inter alia, in linear DNA molecules (e.g., restriction fragments), viruses, plasmids, and chromosomes. In discussing the structure of particular double-stranded DNA molecules, sequences can be described herein according to the normal convention of giving only the sequence in the 5’ to 3’ direction along the non-transcribed strand of DNA (i.e., the strand having a sequence homologous to the mRNA).

[0098] A DNA coding sequence or “coding region” is a double-stranded DNA sequence which is transcribed and translated into a polypeptide in vivo when placed under the control of appropriate expression control sequences. The boundaries of the coding sequence (the “open reading frame” or “ORF”) are determined by a start codon at the 5' (amino) terminus and a translation stop codon at the 3' (carboxyl) terminus. A coding sequence can include, but is not limited to, prokaryotic sequences, cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic (e.g., mammalian) DNA, and synthetic DNA sequences. A polyadenylation signal and transcription termination sequence is, usually, be located 3' to the coding sequence. The term “non-coding sequence” or “non-coding region” refers to regions of a polynucleotide sequence that are not translated into amino acids (e.g. 5' and 3' un-translated regions).

[0099] The term “reading frame” refers to one of the six possible reading frames, three in each direction, of the double stranded DNA molecule. The reading frame that is used determines which codons are used to encode amino acids within the coding sequence of a DNA molecule.

[00100] As used herein, an antisense nucleic acid molecule comprises a nucleotide sequence which is complementary to a sense nucleic acid encoding a protein, e.g., complementary to the coding strand of a double-stranded cDNA molecule, complementary to an mRNA sequence or complementary to the coding strand of a gene. Accordingly, an antisense nucleic acid molecule can hydrogen bond to a sense nucleic acid molecule.

[00101] The term “base pair” or (“bp”): a partnership of adenine (A) with thymine (T), or of cytosine (C) with guanine (G) in a double stranded DNA molecule. In RNA, uracil (U) is substituted for thymine.

[00102] As used herein a “codon” refers to the three nucleotides which, when transcribed and translated, encode a single amino acid residue; or in the case of UUA, UGA or UAG encode a termination signal. Codons encoding amino acids are well known in the art and are provided for convenience herein in Table 1.

Table 1

Codon Usage Table

<td> Codon</td><td> Amino acid</td><td> AA</td><td> Abbr.</td><td> Codon</td><td> Amino acid</td><td> AA</td><td> Abbr.</td>

<td> uuu</td><td> Phenylalanine</td><td> Phe</td><td> F</td><td> ucu</td><td> Serine</td><td> Ser</td><td> S</td>

<td> uuc</td><td> Phenylalanine</td><td> Phe</td><td> F</td><td> ucc</td><td> Serine</td><td> Ser</td><td> S</td>

<td> UUA</td><td> Leucine</td><td> Leu</td><td> L</td><td> UCA</td><td> Serine</td><td> Ser</td><td> s</td>

<td> UUG</td><td> Leucine</td><td> Leu</td><td> L</td><td> UCG</td><td> Serine</td><td> Ser</td><td> s</td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> CUU</td><td> Leucine</td><td> Leu</td><td> L</td><td> ecu</td><td> Proline</td><td> Pro</td><td> P</td>

<td> cue</td><td> Leucine</td><td> Leu</td><td> L</td><td> CCC</td><td> Proline</td><td> Pro</td><td> P</td>

<td> CUA</td><td> Leucine</td><td> Leu</td><td> L</td><td> CCA</td><td> Proline</td><td> Pro</td><td> P</td>

<td> CUG</td><td> Leucine</td><td> Leu</td><td> L</td><td> CCG</td><td> Proline</td><td> Pro</td><td> P</td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> AUU</td><td> Isoleucine</td><td> He</td><td> I</td><td> ACU</td><td> Threonine</td><td> Thr</td><td> T</td>

<td> AUC</td><td> Isoleucine</td><td> He</td><td> I</td><td> ACC</td><td> Threonine</td><td> Thr</td><td> T</td>

<td> AUA</td><td> Isoleucine</td><td> He</td><td> I</td><td> ACA</td><td> Threonine</td><td> Thr</td><td> T</td>

<td> Codon</td><td> Amino acid</td><td> AA</td><td> Abbr.</td><td> Codon</td><td> Amino acid</td><td> AA</td><td> Abbr.</td>

<td> AUG</td><td> Methionine</td><td> Met</td><td> M</td><td> ACH</td><td> Threonine</td><td> Thr</td><td> T</td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> GUU</td><td> Valine</td><td> Vai</td><td> V</td><td> GCU</td><td> Alanine</td><td> Ala</td><td> A</td>

<td> GUC</td><td> Valine</td><td> Vai</td><td> V</td><td> GCC</td><td> Alanine</td><td> Ala</td><td> A</td>

<td> GUA</td><td> Valine</td><td> Vai</td><td> V</td><td> GCA</td><td> Alanine</td><td> Ala</td><td> A</td>

<td> GUG</td><td> Valine</td><td> Vai</td><td> V</td><td> GCG</td><td> Alanine</td><td> Ala</td><td> A</td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> UAU</td><td> Tyrosine</td><td> Tyr</td><td> Y</td><td> UGU</td><td> Cysteine</td><td> Cys</td><td> C</td>

<td> UAC</td><td> Tyrosine</td><td> Tyr</td><td> Y</td><td> UGC</td><td> Cysteine</td><td> Cys</td><td> C</td>

<td> UUA</td><td></td><td> Stop</td><td></td><td> UGA</td><td></td><td> Stop</td><td></td>

<td> UAG</td><td></td><td> Stop</td><td></td><td> UGG</td><td> Tryptophan</td><td> Trp</td><td> w</td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> CAU</td><td> Histidine</td><td> His</td><td> H</td><td> CGU</td><td> Arginine</td><td> Arg</td><td> R</td>

<td> CAC</td><td> Histidine</td><td> His</td><td> H</td><td> CGC</td><td> Arginine</td><td> Arg</td><td> R</td>

<td> CAA</td><td> Glutamine</td><td> Gin</td><td> Q</td><td> CGA</td><td> Arginine</td><td> Arg</td><td> R</td>

<td> CAG</td><td> Glutamine</td><td> Gin</td><td> Q</td><td> CGG</td><td> Arginine</td><td> Arg</td><td> R</td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> AAU</td><td> Asparagine</td><td> Asn</td><td> N</td><td> AGU</td><td> Serine</td><td> Ser</td><td> S</td>

<td> AAC</td><td> Asparagine</td><td> Asn</td><td> N</td><td> AGC</td><td> Serine</td><td> Ser</td><td> s</td>

<td> AAA</td><td> Lysine</td><td> Lys</td><td> K</td><td> AGA</td><td> Arginine</td><td> Arg</td><td> R</td>

<td> AAG</td><td> Lysine</td><td> Lys</td><td> K</td><td> AGG</td><td> Arginine</td><td> Arg</td><td> R</td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> GAU</td><td> Aspartate</td><td> Asp</td><td> D</td><td> GGU</td><td> Glycine</td><td> Gly</td><td> G</td>

<td> GAC</td><td> Aspartate</td><td> Asp</td><td> D</td><td> GGC</td><td> Glycine</td><td> Gly</td><td> G</td>

<td> GAA</td><td> Glutamate</td><td> Glu</td><td> E</td><td> GGA</td><td> Glycine</td><td> Gly</td><td> G</td>

<td> GAG</td><td> Glutamate</td><td> Glu</td><td> E</td><td> GGG</td><td> Glycine</td><td> Gly</td><td> G</td>

[00103] As used herein, a “wobble position” refers to the third position of a codon. Mutations in a DNA molecule within the wobble position of a codon, in some embodiments, result in silent or conservative mutations at the amino acid level. For example, there are four codons that encode Glycine, i.e., GGU, GGC, GGA and GGG, thus mutation of any wobble position nucleotide, to any other nucleotide, does not result in a change at the amino acid level of the encoded protein and, therefore, is a silent substitution.

[00104] Accordingly a “silent substitution” or “silent mutation” is one in which a nucleotide within a codon is modified, but does not result in a change in the amino acid residue encoded by the codon. Examples include mutations in the third position of a codon, as well in the first position of certain codons such as in the codon “CGG” which, when mutated to AGG, still encodes Arg.

[00105] The terms “gene,” “recombinant gene” and “gene construct” as used herein, refer to a DNA molecule, or portion of a DNA molecule, that encodes a protein or a portion thereof. The DNA molecule can contain an open reading frame encoding the protein (as exon sequences) and can further include intron sequences. The term intron as used herein, refers to a DNA sequence present in a given gene which is not translated into protein and is found in some, but not all cases, between exons. It can be desirable for the gene to be operably linked to, (or it can comprise), one or more promoters, enhancers, repressors and/or other regulatory sequences to modulate the activity or expression of the gene, as is well known in the art.

[00106] As used herein, a “complementary DNA” or “cDNA” includes recombinant polynucleotides synthesized by reverse transcription of mRNA and from which intervening sequences (introns) have been removed.

[00107] “Homology” or “identity” or “similarity” refers to sequence similarity between between two nucleic acid molecules. Homology and identity can each be determined by comparing a position in each sequence which can be aligned for purposes of comparison. When an equivalent position in the compared sequences is occupied by the same base, then the molecules are identical at that position; when the equivalent site occupied by the same or a similar nucleic acid residue (e.g., similar in steric and/or electronic nature), then the molecules can be referred to as homologous (similar) at that position. Expression as a percentage of homology/similarity or identity refers to a function of the number of identical or similar nucleic acids at positions shared by the compared sequences. A sequence which is “unrelated” or “non-homologous” shares less than 40% identity, less than 35% identity, less than 30% identity, or less than 25% identity with a sequence described herein. In comparing two sequences, the absence of residues (amino acids or nucleic acids) or presence of extra residues also decreases the identity and homology/similarity.

[00108] The term “homology” describes a mathematically based comparison of sequence similarities which is used to identify genes with similar functions or motifs. The nucleic acid sequences described herein can be used as a “query sequence” to perform a search against public databases, for example, to identify other family members, related sequences or homologs. Such searches can be performed using the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990) J. Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to nucleic acid molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (e.g., XBLAST and BLAST) can be used (See www.ncbi.nlm.nih.gov).

[00109] As used herein, “identity” means the percentage of identical nucleotide residues at corresponding positions in two or more sequences when the sequences are aligned to maximize sequence matching, i.e., taking into account gaps and insertions. Identity can be readily calculated by known methods, including but not limited to those described in (Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J. Applied Math., 48: 1073 (1988). Methods to determine identity are designed to give the largest match between the sequences tested. Moreover, methods to determine identity are codified in publicly available computer programs. Computer program methods to determine identity between two sequences include, but are not limited to, the GCG program package (Devereux, J., et al., Nucleic Acids Research 12(1): 387 (1984)), BLASTP, BLASTN, and FASTA (Altschul, 53

S. F. et al., J. Molec. Biol. 215: 403-410 (1990) and Altschul et al. Nuc. Acids Res. 25: 3389-3402 (1997)). The BLAST X program is publicly available from NCBI and other sources (BLAST Manual, Altschul, S., et al., NCBI NLM NIH Bethesda, Md. 20894; Altschul, S., et al., J. Mol. Biol. 215: 403-410 (1990). The wellknown Smith Waterman algorithm can also be used to determine identity.

[00110] A “heterologous” region of a DNA sequence is an identifiable segment of DNA within a larger DNA sequence that is not found in association with the larger sequence in nature. Thus, when the heterologous region encodes a mammalian gene, the gene can usually be flanked by DNA that does not flank the mammalian genomic DNA in the genome of the source organism. Another example of a heterologous coding sequence is a sequence where the coding sequence itself is not found in nature (e.g., a cDNA where the genomic coding sequence contains introns or synthetic sequences having codons or motifs different than the unmodified gene). Allelic variations or naturally-occurring mutational events do not give rise to a heterologous region of DNA as defined herein.

[00111] The term “transition mutations” refers to base changes in a DNA sequence in which a pyrimidine (cytidine (C) or thymidine (T) is replaced by another pyrimidine, or a purine (adenosine (A) or guanosine (G) is replaced by another purine.

[00112] The term “transversion mutations” refers to base changes in a DNA sequence in which a pyrimidine (cytidine (C) or thymidine (T) is replaced by a purine (adenosine (A) or guanosine (G), or a purine is replaced by a pyrimidine.

Nucleic acid prodrugs comprising a chiral X-phosphonate moiety [00113] The general principles of prodrug design are outlined by Bundgard (Design and Application of Prodrugs. In a Textbook of Drug Design and Development; Krogsgaard-Larsen, P., Bundgard, H., Eds.; Harwood: Reading, UK, 1991).

[00114] One strategy to improve the pharmaceutical properties of molecules with desirable biological activity but poor pharmaceutical properties is to administer the molecule of interest as a prodrug derivative. These prodrugs can exhibit one or more of the properties of increased oral bioavailability, increased cell permeability, increased water solubility, reduced first-pass effect, increased stability, active transport by intestinal transporters, or avoidance of efflux transporters, when compared to the parent molecule.

[00115] Oligonucleotides have several pharmaceutical properties which can be improved through the application of prodrug strategies. In particular, oligonucleotides are rapidly degraded by nucleases and exhibit poor cellular uptake through the cytoplasmic cell membrane (Poijarvi-Virta et al., Curr. Med. Chem. (2006), 13(28);3441 -65; Wagner et al., Med. Res. Rev. (2000), 20(6):417-51; Peyrottes et al., Mini Rev. Med. Chem. (2004), 4(4):395-408; Gosselin et al., (1996), 43(1): 196-208; Bologna et al., (2002), Antisense &amp; Nucleic Acid Drug Development 12:33-41). In one example, Vives et al., (Nucleic Acids Research (1999), 27(20):4071-76) found that tert-butyl SATE pro-oligonucleotides displayed markedly increased cellular penetration compared to the parent oligonucleotide.

[00116] In some embodiments, the prodrug moiety is removed selectively by esterases, nucleases or a cytochrome P450 enzyme, including but not limited to those listed below.

<td> Family</td><td> Gene</td>

<td> CYP1</td><td> CYP1A1, CYP1A2, CYP1B1</td>

<td> CYP2</td><td> CYP2A6, CYP2A7, CYP2A13, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1, CYP2F1, CYP2J2, CYP2R1, CYP2S1, CYP2U1, CYP2W1</td>

<td> CYP3</td><td> CYP3A4, CYP3A5, CYP3A7, CYP3A43</td>

<td> CYP4</td><td> CYP4A11, CYP4A22, CYP4B1, CYP4F2, CYP4F3, CYP4F8, CYP4F11, CYP4F12, CYP4F22, CYP4V2, CYP4X1, CYP4Z1</td>

<td> CYP5</td><td> CYP5A1</td>

<td> CYP7</td><td> CYP7A1, CYP7B1</td>

<td> CYP8</td><td> CYP8A1 (prostacyclin synthase), CYP8B1 (bile acid biosynthesis)</td>

<td> CYP11</td><td> CYP11A1, CYP11B1, CYP11B2</td>

<td> CYP17</td><td> CYP17A1</td>

<td> CYP19</td><td> CYP19A1</td>

<td> CYP20</td><td> CYP20A1</td>

<td> CYP21</td><td> CYP21A2</td>

<td> CYP24</td><td> CYP24A1</td>

<td> CYP26</td><td> CYP26A1, CYP26B1, CYP26C1</td>

<td> CYP27</td><td> CYP27A1 (bile acid biosynthesis), CYP27B1 (vitamin D3 1-alpha hydroxylase, activates vitamin D3), CYP27C1 (unknown function)</td>

<td> CYP39</td><td> CYP39A1</td>

<td> CYP46</td><td> CYP46A1</td>

<td> CYP51 ... .....</td><td> CYP51A1 (lanosterol 14-alphademethylase)</td>

[00117] In some embodiments, the prodrug is removed when the prooligonucleotide has not yet been transported through the cell membrane. In other embodiments, the prodrug is removed from the prooligonucleotide only after it is transported through the cell membrane. Alternatively, the prodrug is removed only after it is transported into an organelle within the cell. In some embodiments, the prodrug moiety is removed through a non-enzymatic removal including but not limited to the spontaneous reduction inside the cell.

[00118] Described herein are prodrugs of a nucleic acid comprisng a modification of a chiral Xphosphonate, wherein the modification improves one or more physicochemical, pharmacokinetic or pharmacodynamic property of the nucleic acid. A prodrug moiety is connected to an oxygen or sulfur atom which is connected to the phosphorus atom of a phosphonate or phosphothiorate group of the nucleotide. The prodrug moiety includes but is not limited to S-acyl-2-thioethyl, acyloxy, thioacyloxy, 2-carboalkoxyethyl, disulfide, thiaminal, and enol ester derivatives.

[00119] In one embodiment, the prodrug moiety is an S-acyl-2-thioethyl moiety having the following structure:

<img file="IN720DEN2012A_D0223.tif" />

wherein Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl. In some embodiments, Rn is methyl, ethyl or cyclopropyl.

[00120] In other embodiments, the prodrug moiety is an acyloxy moiety having following structure:

<img file="IN720DEN2012A_D0224.tif" />

wherein R_n is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl, and R_J2 is hydrogen or alkyl. In some embodiments, Rn is methyl and R_n is hydrogen.

[00121] Alternatively, the prodrug moiety is a thioacyloxy moiety having the following structure:

Rf2 O /''sAAr,, wherein Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl, and R_n is hydrogen or alkyl. In some embodiments, Rn is methyl and Ri₂ is hydrogen.

[00122] The invention also provides a prodrug 2-carboalkoxyethyl moeity having one of the following structures:

<img file="IN720DEN2012A_D0225.tif" />

or wherein R¹⁰ is an alkyl group having 1 to 4 carbon atoms. In some embodiments, R¹⁰ is methyl or ethyl. [00123] In yet other embodiments, the prodrug moiety is a disulfide moiety having the following structure:

<img file="IN720DEN2012A_D0226.tif" />

wherein Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl. In some embodiments, Rn is methyl, ethyl or benzyl.

[00124] In further embodiments, the prodrug moiety is a thioacetal moiety having the following structure:

<img file="IN720DEN2012A_D0227.tif" />

wherein R¹⁰ is an alkyl group having 1 to 4 carbon atoms and Ri i is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl. In some embodimenst, R¹⁰ is methyl and Rn is methyl or phenyl.

[00125] The invention also provides enol ester prodrug moieties having one of the following structures:

<img file="IN720DEN2012A_D0228.tif" />

C4 enol ester

C3 enol ester wherein Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl. In some embodiments, the C3- enol ester prodrug moiety or the C4 enol ester prodrug moiety is in the cis form. In some embodiments of the C3- enol ester prodrug moiety or the C4 enol ester prodrug moiety, Rn is methyl, ethyl or phenyl.

[00126] In one embodiment, the prodrug moiety is a trialkylammoniumethyl moiety having one of the following structures:

<img file="IN720DEN2012A_D0229.tif" />

<img file="IN720DEN2012A_D0230.tif" />

<img file="IN720DEN2012A_D0231.tif" />

[00127] In one embodiment, the prodrug moiety is a alkylhydroxamate moiety having one of the following structures:

<img file="IN720DEN2012A_D0232.tif" />

<img file="IN720DEN2012A_D0233.tif" />

O , or O [00128] In one embodiment, the prodrug moiety is a acylhydroxamate moiety having one of the following structures:

<img file="IN720DEN2012A_D0234.tif" />

<img file="IN720DEN2012A_D0235.tif" />

(j) Me (j) Me

<img file="IN720DEN2012A_D0236.tif" />

, or O [00129] One embodiment provides a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0237.tif" />

;a

OR³

Formula 1 wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaiyl-Y¹-, -P(O)(R^C)2, -HP(O)(R^e), -OR* or -SR^C;

Y¹ is O, NR^d, S, or Se;

R is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y² is O, NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OR^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

at least one instance of X is -OCH₂CH₂S-S(O)₂Ri₀, -OCH₂CH₂S-SCH₂CH₂OH, -OCH₂CH₂CO₂H,

R12 Q

<img file="IN720DEN2012A_D0238.tif" />

<img file="IN720DEN2012A_D0239.tif" />

<img file="IN720DEN2012A_D0240.tif" />

&gt;

<img file="IN720DEN2012A_D0241.tif" />

<img file="IN720DEN2012A_D0242.tif" />

<img file="IN720DEN2012A_D0243.tif" />

<img file="IN720DEN2012A_D0244.tif" />

<img file="IN720DEN2012A_D0245.tif" />

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety

<img file="IN720DEN2012A_D0246.tif" />

connected to a nucleic acid;

Rio is an alkyl group having 1 to 4 carbon atoms;

Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl;

Z is S or O;

q is 0, 1, or 3;

w is 1,2,3,4,5, or 6;

R₁₅ and Ri6 are independently hydrogen or methyl;

R₁₇ is selected from alkyl, aryl or a CH₂CH=CH₂;

Ris is selected from N(CH₃)₂,

<img file="IN720DEN2012A_D0247.tif" />

<img file="IN720DEN2012A_D0248.tif" />

<img file="IN720DEN2012A_D0249.tif" />

, and

<img file="IN720DEN2012A_D0250.tif" />

^Me02^C ; and n is an integer of 1 to about 200.

[00130] In one aspect the invention provides a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0251.tif" />

OR³

Formula 1 wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OP(OXR^e)2, -HP(O)(R^C), -OR^a or -SR^C;

Y¹ is O, NR^d, S, or Se;

R^a is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y² is O, NR^d, or S;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OR^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

<img file="IN720DEN2012A_D0252.tif" />

<img file="IN720DEN2012A_D0253.tif" />

<img file="IN720DEN2012A_D0254.tif" />

<img file="IN720DEN2012A_D0255.tif" />

Ο , or Ο

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid;

R¹⁰ is an alkyl group having 1 to 4 carbon atoms;

Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl; and n is an integer of 1 to about 200.

[00131] In one aspect the invention provides a nucleic acid prodrug having the following structure:

<img file="IN720DEN2012A_D0256.tif" />

wherein R¹ is -OH, -SH, -NR^dR^d, -N3, halogen, hydrogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -OP(OXR^e)2, -HP(O)(R^e), -OR* or -SR^C;

Y¹ is O, NR^d, S, orSe;

R“ is a blocking group;

R^c is a blocking group;

each instance of R^d is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, -P(O)(R^e)₂, or -HP(O)(R^e);

each instance of R^e is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y²-, alkenyl-Y²-, alkynylY²-, aryl-Y²-, or heteroaryl-Y²-, or a cation which is Na⁺¹, Li⁺¹, or K⁺¹;

Y²is 0,NR^d, orS;

each instance of R² is independently hydrogen, -OH, -SH, -NR^dR^d, -N₃, halogen, alkyl, alkenyl, alkynyl, alkyl-Y¹-, alkenyl-Y¹-, alkynyl-Y¹-, aryl-Y¹-, heteroaryl-Y¹-, -0R^b ,or -SR^C, wherein R^b is a blocking group; each instance of Ba is independently a blocked or unblocked adenine, cytosine, guanine, thymine, uracil or modified nucleobase;

<img file="IN720DEN2012A_D0257.tif" />

<img file="IN720DEN2012A_D0258.tif" />

<img file="IN720DEN2012A_D0259.tif" />

<img file="IN720DEN2012A_D0260.tif" />

, or Ο

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid;

R¹⁰ is an alkyl group having 1 to 4 carbon atoms;

Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl; and n is an integer of 1 to about 200.

[00132] A further embodiment provides the nucleic acid prodrug of Formula 1 or Formula 2, wherein each X moiety of the nucleic acid prodrug is independently selected from -OCH2CH2S-S(O)2R10, -OCH2CH2SSCH2CH2OH, -OCH2CH2CO2H,

<img file="IN720DEN2012A_D0261.tif" />

<img file="IN720DEN2012A_D0262.tif" />

<img file="IN720DEN2012A_D0263.tif" />

<img file="IN720DEN2012A_D0264.tif" />

<img file="IN720DEN2012A_D0265.tif" />

<img file="IN720DEN2012A_D0266.tif" />

<img file="IN720DEN2012A_D0267.tif" />

<img file="IN720DEN2012A_D0268.tif" />

<img file="IN720DEN2012A_D0269.tif" />

<img file="IN720DEN2012A_D0270.tif" />

<img file="IN720DEN2012A_D0271.tif" />

<img file="IN720DEN2012A_D0272.tif" />

<img file="IN720DEN2012A_D0273.tif" />

<img file="IN720DEN2012A_D0274.tif" />

R³ is hydrogen, a blocking group, a linking moiety connected to a solid support or a linking moiety connected to a nucleic acid;

Rio is an alkyl group having 1 to 4 carbon atoms;

Rn is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl;

R12 is hydrogen or alkyl;

Z is S or O;

q is 0, 1, or 3; w is 1,2,3,4,5, or 6;

Ru and R_J6 are independently hydrogen or methyl;

Rn is selected from alkyl, aryl or a CH₂CH=CH2; and

Ris is selected from N(CH₃)₂,

<img file="IN720DEN2012A_D0275.tif" />

<img file="IN720DEN2012A_D0276.tif" />

<img file="IN720DEN2012A_D0277.tif" />

MeC^C [00133]In some embodiments, n is an integer of 1 to about 50; 1 to about 40; 1 to about 30; 1 to about 25; 1 to about 20; 1 to about 15; or 1 to about 10.

[00134] One embodiment provides a non-racemic pro-oligonucleotide wherein the pro-oligonucleotide is an analog of 2-5 A, having a structure of the following formula:

<img file="IN720DEN2012A_D0278.tif" />

Formula A₃-l wherein X is any of the prodrug moieties descibed herein.

[00135] In some embodiments, the non-racemic pro-oligonucleotide is a 2-5A analog having the following structure:

<img file="IN720DEN2012A_D0279.tif" />

Formula A₃-2 wherein R_n is alkyl, aryl, heteroaryl, heterocyclyl, or cycloalkyl.

[00136] In one embodiment, non-racemic pro-oligonucleotide is a 2-5A analog having the following structure:

<img file="IN720DEN2012A_D0280.tif" />

H0 0H

Formula A₃-3

EXEMPLARY METHODS OF SYNTHESIS

General discussion of the methods of synthesis of nucleic acid prodrugs comprising a chiral Xphosphonate moiety [00137] The methods described herein provide for an efficient synthesis of phosphorus atom-modified nucleic acid prodrugs wherein the stereochemical configuration at a phosphorus atom is controlled, thus producing a stereodefined oligonucleotide. While the exemplary methods of synthesis described herein provide for a 3’-5’ nucleotide likage, the 2’-5’ nucleotide linkage is also contemplated.

[00138] The pro-oligonucleotides of the invention may be synthesized by modifying either a chiral phosphorothioate or chiral H-phosphonate of a nucleotide or nucleic acid.

[00139] A S-acyl- 2-thioethyl pronucleotide may be synthesized from a nucleic acid or nucleotide comprising a chiral H-phosphonate as shown in the following scheme:

Scheme 1

<img file="IN720DEN2012A_D0281.tif" />

[00140] In some embodiments, Rl is -OP(O)(Re)2, wherein Re is θ . The chiral Hphosphonate is treated with N-chlorosuccinimde and then reacted with S-acyl- 2-thioethyl alcohol to produce a S-acyl- 2-thioethyl prodrug. Protecting groups present at R¹, R², and/or R³ may be subsequently removed.

[00141] An acyloxy nucleic acid prodrug may be synthesized from a nucleic acid or nucleotide comprising a chiral H-phosphonate as shown in the following scheme:

Scheme 2

<img file="IN720DEN2012A_D0282.tif" />

<img file="IN720DEN2012A_D0283.tif" />

[00142] The chiral H-phosphonate is treated with N-chlorosuccinimide and then reacted with a hydroxymethyl acetate compound to produce an acyloxy prodrug. Protecting groups present at R¹, R², and/or R³ may be subsequently removed.

[00143] A thioacyloxy nucleic acid prodrug may be synthesized from a nucleic acid or nucleotide comprising a chiral phosphorothioate as shown in the following scheme:

Scheme 3

<img file="IN720DEN2012A_D0284.tif" />

Ba

<img file="IN720DEN2012A_D0285.tif" />

[00144] The chiral phosphorothioate is treated with a chloromethyl acyloxy compound to produce an acyloxy prodrug. Protecting groups present at R¹, R², and/or R³ may be subsequently removed.

[00145] A 2-carboalkoxyethyl nucleic acid prodrug may be synthesized from a nucleic acid or nucleotide comprising a chiral phosphorothioate as shown in the following scheme:

Scheme 4

<img file="IN720DEN2012A_D0286.tif" />

[00146] The deprotonated chiral phosphorothioate is reacted with an alkyl acrylate to produce a 2carboalkoxyethyl pronucleotide. Protecting groups present at R¹, R², and/or R³ may be subsequently removed.

[00147] A disulfide nucleic acid prodrug may be synthesized from a nucleic acid or nucleotide comprising a chiral phosphorothioate as shown in the following scheme:

<img file="IN720DEN2012A_D0287.tif" />

The deprotonated chiral phosphorothioate is reacted with a dialkyl sulfide to produce an alkyl disulfide pronucleotide. Protecting groups present at R¹, R², and/or R³ may be subsequently removed.

[00148] A thioacetal nucleic acid prodrug may be synthesized from a nucleic acid or nucleotide comprising a chiral phosphorothioate as shown in the following scheme:

Scheme 6

<img file="IN720DEN2012A_D0288.tif" />

[00149] A 1,1,-dialkyoxy 3-acyloxy propane is reacted with trimethylsilyltriflate and the deprotonated chiral phosphorothioate is then added to the reaction mixture to produce an thioacetal pronucleotide. Protecting groups present at R¹, R², and/or R³ may be subsequently removed.

[001501A C3 enol ester nucleic acid prodrug may be synthesized from a nucleic acid or nucleotide comprising a chiral phosphorothioate as shown in the following scheme:

Scheme 7

<img file="IN720DEN2012A_D0289.tif" />

OR³ OR³ [00151[The deprotonated chiral phosphorothioate is reacted with an (E) -3- chloro- 1-acyloxy-prop-l-ene compound to produce the C3 enol ester nucleic acid prodrug. Protecting groups present at R¹, R², and/or R³may be subsequently removed.

[00152] A C4 enol ester nucleic acid prodrug may be synthesized from a nucleic acid or nucleotide comprising a chiral phosphorothioate as shown in the following scheme:

Scheme 8

<img file="IN720DEN2012A_D0290.tif" />

[00153] The deprotonated chiral phosphorothioate is reacted with an (E) -3- chloro- 1-acyloxy-but-l-ene compound to produce the C3 enol ester nucleic acid prodrug. Protecting groups present at R¹, R², and/or R³may be subsequently removed.

[00154] In some embodiments the nucleic acid comprising a chiral phosphorothioate or chiral H-phosphonate is synthesized as described herein. In other embodiments, other methods of synthesis may be used to provide the nucleic acid comprising a chiral phosphorothioate or chiral H-phosphonate.

Scheme 9. Synthesis of chiral phosphorothioate precursors of the prodrug oligonucleotides of the invention. (Route A)

<img file="IN720DEN2012A_D0291.tif" />

<img file="IN720DEN2012A_D0292.tif" />

[00155] The reaction of a molecule comprising an achiral //-phosphonate moiety of Formula 2 with a nucleoside comprising nucleophilic moiety of Formula IV results in the formation of a condensed intermediate (V); which is converted to a nucleic acid comprising a chiral X'-phosphonate moiety which can be further modified to produce the prodrug oligonucleotide of Formula I comprising a chiral X-phosphonate moiety. The synthesis of the condensed intermediate comprises the steps of (a) activation of the compound of Formula 2 with a condensing agent to form intermediate II, (b) reaction with a chiral reagent to form intermediate III, followed by (c) reaction with the compound of Formula IV.

[00156] The condensed intermediate may be converted to a nucleic acid comprising a chiral X' phosphonate moiety of Formula Γ by capping the chiral auxiliary with a moiety A, which is an acyl, aryl, alkyl, aralkyl, or silyl moiety, and modifying the phosphorus to introduce J, which is S, Se, or BH₃, thus producing a compound of Formula VII.

[00157] The compound of Formula VII may be converted to the compound of Formula Γ, where X' is S, Se, or BH₃, and n is 1, by cleaving the chiral reagent, and deblocking blocking groups and cleaving from solid support if desired. Alternatively the compound of Formula VII is subjected to chain elongation by deblocking the 5’ terminus, and repeating coupling steps to produce a condensed intermediate as before. The steps of capping, modifying, deblocking, and chain elongation are repeated until the desired n is achieved. At that point, the chiral reagents at each phosphonate are cleaved, the remaining blocking groups are cleaved, including cleaving from a solid support, if desired, to produce the compound of Formula Γ, where X' is S, Se, or BH₃, and n is greater than or equal to 2 and less than about 200. The compound of Formula Γ, where X' is S is then converted by the methods described herein to form the pro-oligonucleotide compound of Formula 1.

Modifying agents used to introduce S, Se, or BH₂ at chiral phosphorus of the condensed intermediate V in Route A.

[00158] In some embodiments, the modifying agent is a sulfur electrophile, selenium electrophile, or boronating agent. In some embodiments, the sulfur electrophile is a compound having one of the following formulas:

S₈ (Formula B), Z¹⁰-S-S-Zⁿ, or Z^,o-S-X-Z“, wherein Z¹⁰ and Z¹¹ are independently alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaryloxy, acyl, amide, imide, or thiocarbonyl, or Z¹⁰ and Z¹¹ are taken together to form a 3 to 8 membered alicyclic or heterocyclic ring, which may be substituted or unsubstituted; X is SO₂, O, or NR^f; and R^f is hydrogen, alkyl, alkenyl, alkynyl, or aryl. In other embodiments, the sulfur electrophile is a compound of Formula B, C, D, E, or F:

S₈

Formula B

<img file="IN720DEN2012A_D0293.tif" />

Formula C

<img file="IN720DEN2012A_D0294.tif" />

Formula D

<img file="IN720DEN2012A_D0295.tif" />

Formula E Formula F [00159] In other embodiments, the sulfur electrophile is Formula F, Formula E or Formula B.

[00160] In some embodiments, the selenium electrophile is a compound having one of the following formulas:

Se (Formula G), Z¹⁰-Se-Se-Zⁿ, or Z'^Se-X-Z¹¹, wherein Z¹⁰ and Z¹¹ are independently alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaryloxy, acyl, amide, imide, or thiocarbonyl, or Z¹⁰ and Z¹¹ are taken together to form a 3 to 8 membered alicyclic or heterocyclic ring, which may be substituted or unsubstituted; X is SO₂, S, O, or NR^f; and R^f is hydrogen, alkyl, alkenyl, alkynyl, or aryl. [00161] In other embodiments, the selenium electrophile is a compound of Formula G, Η, I, J, K, or L.

<img file="IN720DEN2012A_D0296.tif" />

Formula G Formula H Formula I Formula J Formula K Formula L [00162] In some embodiments, the selenium electrophile is Formula G or Formula L.

[00163] In some embodiments, the boronating agent is borane-AUV-diisopropylethylamine (BH₃-DIPEA), borane-pyridine (BH₃-Py), borane-2-chloropyridine (BH₃-CPy), borane-aniline (BH₃-An), borane70 tetrahydrofuran (BH₃-THF), or borane-dimethylsulfide (BH₃-Me₂S), aniline-cyanoborane, triphenylphosphine-carboalkoxyboranes.

[00164] In other embodiments, the boronating agent is borane-AT^V-diisopropylethylamine (BH3-DIPEA), borane-2-chloropyridine (BH3-CPy), borane-tetrahydrofuran (BH3-THF), or borane-dimethylsulfide (BH3-Me2S).

Scheme 10.

Synthesis of a precursor of a prodrug oligonucleotide via chiral H-phosphonates (Route B).

<img file="IN720DEN2012A_D0297.tif" />

Formula 1 [00165] In another embodiment, described in Scheme 10, an achiral 77-phosphonate of Formula 2 is treated with a condensing reagent to form an intermediate of structure II. The intermediate of structure II is not isolated and is treated in the same pot with a chiral reagent to form a chiral intermediate of structure III. The intermediate of structure III is not isolated and undergoes a reaction in the same pot with a nucleoside or modified nucleoside of structure IX to provide a chiral phosphite compound of structure X. In some embodiments, structure X is extracted into a solvent to separate it from side products, impurities, and/or reagents. In other embodiments, when the method is performed via solid phase synthesis, the solid support comprising the compound of structure X is filtered away from side products, impurities, and/or reagents. The compound of structure X is treated with an acid to remove the blocking group at the 5'-end of the growing nucleic acid chain (structure XI). The acidification step also removes the chiral auxiliary ligand to provide a chiral H-phosphonate IX. The 5'-deblocked intermediate is optionally allowed to re-enter the 71 chain elongation cycle to form a condensed intermediate containing a blocked 5'-end, which is then acidified to remove the 5'-end blocking group and chiral auxiliary ligand.

[00166] When the desired chain length has been reached, the 5'-deprotected intermediate undergoes a modifying step to introduce a moiety X bonded to each of the phosphorus atoms to provide a compound of structure XII. The modified intermediate is deblocked by removal of remaining protecting groups, e.g., nucleobase, modified nucleobase, sugar or modified sugar protecting groups are removed, to provide a nucleic acid of Formula 1. In embodiments where a solid support is used, the phosphorus-atom modified nucleic acid is then cleaved from the solid support. In certain embodiments, the nucleic acids is left attached on the solid support for purification purposes and then cleaved from the solid support following purification. In one embodiment, the synthesis described in Scheme 10 is useful when the G1 and G2 positions of the chiral auxiliary ligand of Formula A are not hydrogen.

Modification of the compound of Formula IX obtained via Route B to introduce an X- or X’phosphonate moiety.

[00167] Other methods used to modify the compound of Formula IX obtained via Route B are illustrated in Reaction Schemes 10a and 10b. Phosphonate and phosphite are known to tautomerize and exist in equilibrium. The phosphite tautomer is less stable than the phosphonate tautomer. Equilibrium lies toward the phosphonate tautomer under neutral conditions due to the very strong P=O bond. Under acidic conditions, the phosphoryl group of the phosphonate becomes reversibly protonated. Cleavage of the P-H bond in the intermediate occurs slowly to produce the phosphite intermediate. Structure IX is then modified to form structure XII, using reagents shown in Reaction Schemes 10a and 10b.

Reaction Scheme 10a.

Modification of the phosphorus center of intermediates synthesized via Route B, using an initial halogenation at phosphorus.

<img file="IN720DEN2012A_D0298.tif" />

<img file="IN720DEN2012A_D0299.tif" />

Reaction Scheme 10b.

Modification of phosphorus in intermediates synthesized via Route B, using an initial silylation.

<img file="IN720DEN2012A_D0300.tif" />

<img file="IN720DEN2012A_D0301.tif" />

<img file="IN720DEN2012A_D0302.tif" />

<img file="IN720DEN2012A_D0303.tif" />

<img file="IN720DEN2012A_D0304.tif" />

\ sulfur selenium I 'electrophile electrophile

<img file="IN720DEN2012A_D0305.tif" />

m

<img file="IN720DEN2012A_D0306.tif" />

<img file="IN720DEN2012A_D0307.tif" />

[00168] In some embodiments, the modifying step is performed by reacting structure IX with a halogenating reagent followed by reacting with a nucleophile. In specific embodiments, the halogenating reagent is CC14, CBr4, CI4„ C12, Br2, 12, sulfuryl chloride (SO2C12), phosgene, triphosgene, sulfur monochloride, sulfur dichloride, chloramine, CuC12, JV-chlorosuccinimide (NCS), 77-bromosuccinimide (NBS), or Niodosuccinimide (NIS). In other specific embodiments, the halogenating reagent is CC14, CBr4, C12, sulfuryl chloride (SO2C12), or ΛΓ-chlorosuccinimide (NCS). In some embodiments, the nucleophile is primary or secondary amines, alcohols, or thiols. In other embodiments, the nucleophile is NR^fR^fH, R^fOH, or R^fSH, wherein R^f is hydrogen, alkyl, alkenyl, alkynyl, or aryl, and at least one of R^f of NR^fR^fH is not hydrogen.

[00169] The modifying step can also be performed by reacting structure IX with a silylating reagent followed by reaction with a sulfur electrophile, a selenium electrophile, a boronating agenζ an alkylating agent, an aldehyde, or an acylating agent.

[00170] In specific embodiments, the silylating reagent is chlorotrimethylsilane (TMS-C1), triisopropylsilylchloride (TIPS-C1), i-butyldimethylsilylchloride (TBDMS-C1), i-butyldiphenylsilylchloride (TBDPS-C1), 1,1,1,3,3,3-hexamethyldisilazane (HMDS), 77-trimethylsilyldimethylamine (TMSDMA), N trimethylsilyldiethylamine (TMSDEA), Ύ-trimethylsilylacetamide (TMSA), JV,Obis(trimethylsilyl)acetamide (BSA), or Y,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA).

[00171] In other specific embodiments, the sulfur electrophile is a compound having one of the following formulas:

S₈ (Formula B), Z¹⁰-S-S-Zⁿ, or Z¹⁰-S-X-Zⁿ, wherein Z¹⁰ and Zⁿ are independently alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaryloxy, acyl, amide, imide, or thiocarbonyl, or Z¹⁰ and Z¹¹ are taken together to form a 3 to 8 membered alicyclic or heterocyclic ring, which may be substituted or unsubstituted; X is SO2, O, or NR^f; and R^f is hydrogen, alkyl, alkenyl, alkynyl, or aryl. In other embodiments, the sulfur electrophile is a compound of Formula B, C, D, E, or F:

<td> Se</td><td> nh₂</td><td></td><td></td><td>⁷ s-s / λ™</td>

<td> Formula B</td><td> Formula C</td><td> Formula D</td><td> Formula E</td><td> Formula F</td>

[00172] In other embodiments, the sulfur electrophile is Formula F, Formula E or Formula B.

[00173] In some embodiments, selenium electrophile is a compound having one of the following formulas: Se (Formula G), Z¹⁰-Se-Se-Zⁿ, or Z¹⁰-Se-X-Zⁿ, wherein Z¹⁰ and Z¹¹ are independently alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaiyloxy, acyl, amide, imide, or thiocarbonyl, or Z¹⁰ and Z¹¹ are taken together to form a 3 to 8 membered alicyclic or heterocyclic ring, which may be substituted or unsubstituted; X is SO₂, S, O, or NR^f; and R^f is hydrogen, alkyl, alkenyl, alkynyl, or aryl. [00174] In other embodiments, the selenium electrophile is a compound of Formula G, Η, I, J, K, or L.

<td> Se</td><td> KSeCN</td><td> Ph-I-Ph Ph</td><td> 0^</td><td>^P\ Se-ββ Ph</td><td> NC se-S^</td>

<td> Formula G</td><td> Formula H</td><td> Formula I</td><td> Formula J</td><td> Formula K</td><td> Formula L</td>

[00175] In some embodiments, the selenium electrophile is Formula G or Formula L.

[00176] In some embodiments, the boronating agent is borane-JVJV-diisopropylethylamine (BH3-DIPEA), borane-pyridine (BH3-Py), borane-2-chloropyridine (BH3-CPy), borane-aniline (BH3-An), boranetetrahydrofuran (BH3-THF), or borane-dimethylsulfide (BH3-Me2S), aniline-cyanoborane, triphenylphosphine-carboalkoxyboranes. In other embodiments, the boronating agent is borane-AQVdiisopropylethylamine (BH₃-DIPEA), borane-2-chloropyridine (BH₃-CPy), borane-tetrahydrofuran (BH₃ THF), or borane-dimethylsulfide (BH3-Me₂S).

[00177] In other embodiments, the alkylating agent is an alkyl halide, alkenyl halide, alkynyl halide, alkyl sulfonate, alkenyl sulfonate, or alkynyl sulfonate.

[00178] In other embodiments, the aldehyde is (para)-formaldehyde, alkyl aldehyde, alkenyl aldehyde, alkynyl aldehyde, or aryl aldehyde.

[00179] In yet other embodiments, the acylating agent is a compound of Formula M or N:

O 0 0

Λ Λ g⁷^m g⁷'xd^/(3⁷

Formula M Formula N (00180] wherein G⁷ is alkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, or heteroaryloxy; and M is F, Cl, Br, I, 3-nitro-l,2,4-triazole, imidazole, alkyltriazole, tetrazole, pentafluorobenzene, or 1-hydroxybenzotriazole.

Scheme 11. Synthesis of a chiral dinucleoside phosphorothioate by stereoselective synthesis.

TBDPS

<img file="IN720DEN2012A_D0308.tif" />

<img file="IN720DEN2012A_D0309.tif" />

<img file="IN720DEN2012A_D0310.tif" />

TBDPSO

O. .^DBLT

Ph

TBDPSO [00181] One method of stereoselective stereochemically pure 3’-phosphoramidites as described by Oka et al, (J. Am. Chem. Soc. (2003), 125:830717). As shown in the Scheme 6a (above), 2-chlorooxazaphospholidine derivatives are allowed to react with a 5’-O-(TBDPS)nucleoside to afford the 3’-0-oxazaphospholidine derivative. Reaction of a 3’-O dinucleoside phosphorothioate synthesis involves the use of (TBDPS)nucleoside with the 3’-0-oxazaphospholidine derivative in the presence of an activator such as N(cyanomethyl)pyrrolidine gives the dinucleoside phosphite as a single diastereomer. The dinucleoside phosphite can be converted to the phosphorothioate by a three-step process involving acetylation with acetic anhydride, sulfurization with the Beaucage reagent (3H-l,2-benzodithiol-3-one-l,l-dioxide; Iyer et al, J. Am.

Chem. Soc. (1990), 112:1253-4), and cleavage of the chiral auxiliary with excess DBU. The protected dinucleoside phosphorthioate is then converted to the prodrug by the methods disclosed herein.

[00182] Other methods useful for the synthesis of dinucleoside phosphorthioates include enzymatic methods (Hacia et al. Biochemistry (1994), 33:5367-9; Tang et al. Nucleosides Nucleotides (1995), 14:985-990), methods involving separation of diasteromeric phosphorthioate mixtures prepared by non-stereoselective methods (Zon et al Oligonucleotides and Analogues: A Practical Approach·, IRL Press: London, 1991, pp

87-108) and methods involving stereoselective synthesis of phosphorthioates (Wilk et al. J. Am. Chem. Soc.

2000, 122, 2149-2156; Lu et al, Angew. Chem., Int. Ed. 2000, 39, 4521-4524; Iyer et al Tetrahedron:Asymmetry 1995, 6, 1051-1054. Iyer et al Tetrahedron Lett. 1998, 39, 2491-2494; Lu et al Tetrahedron 2001, 57, 1677-1687. Stec et al Nucleic Acids Res. 1991, 19, 5883-5888; Stec et al J. Am. Chem. Soc. 1995,117,12019-12029; Uznan'ski etal J. Am. Chem. Soc. 1992, 114, 10197-10202.

Reverse 5' to 3' Nucleic Acid Synthesis [00183] A nucleic acid of Formula 1 comprising a chiral X-phosphonate moiety alternatively is synthesized from the 5' to 3' direction. In embodiments where a solid support is used, the nucleic acid is attached to the solid support through its 5' end of the growing nucleic acid, thereby presenting its 3' group for reaction, including enzymatic reaction (e.g. ligation and polymerization). In some embodiments, this orientation is engineered by preparing nucleoside monomers comprising an achiral 77-phosphonate moiety at the 5' position and protected hydroxyl group at the 3' position. In an embodiment, the nucleic acid is synthesized according to Scheme 12. In Scheme 12, -R4 is -ORb as defined above or, in the last cycle of synthesis, is R⁴, which is equivalent to R¹ as defined herein.

Scheme 12.

5' to 3' synthesis of a pro-oligonucleotide of Formula 1 comprising a chiral X-phosphonate moiety.

<img file="IN720DEN2012A_D0311.tif" />

Formula 1_r [00184] In the embodiment described in Scheme 12, an achiral H-phosphonate of structure Ir is treated with a condensing reagent to form an intermediate of structure Hr. The intermediate of structure Hr is not isolated and is treated in the same pot with a chiral reagent to form an intermediate of structure Illr. The intermediate of structure Illr is not isolated and undergoes a reaction in the same pot with a nucleoside or modified nucleoside of structure XIII to provide a chiral phosphite compound of structure XIV. In some embodiments, structure XIV is extracted into a solvent to separate it from side products, impurities, and/or reagents. In other embodiments, when the method is performed via solid phase synthesis, the solid support comprising the compound of structure XIV is filtered away from side products, impurities, and/or reagents. The compound of structure XIV is treated with an acid to remove the blocking group at the 3'-end of the growing nucleic acid chain (structure XV). The acidification step also removes the chiral auxiliary ligand to provide a compound of structure XIII. The 3'-deblocked intermediate is optionally allowed to re-enter the chain elongation cycle to form a condensed intermediate containing a blocked 3'-end, which is then acidified to remove the 3'-end blocking group and chiral auxiliary ligand. Following at least one round of chain elongation cycle, the 3'-deprotected intermediate undergoes a modifying step to introduce a moiety X bonded to each of the phosphorus atoms to provide a compound of structure XVI. The modified intermediate is deblocked by removal of remaining protecting groups, e.g., nucleobase, modified nucleobase, sugar or modified sugar protecting groups are removed, to provide a nucleic acid of Formula 1. In other embodiments, the nucleoside comprising a 3'-OH moiety is an intermediate from a previous chain elongation cycle as described herein. In yet other embodiments, the nucleoside comprising a 3'-OH moiety is an intermediate obtained from another known nucleic acid synthetic method. After a cycle of synthesis with the first nucleoside, nucleosides, nucleotides, or nucleic acids that contain an unprotected -OH moiety can be used for subsequent elongation cycles. In embodiments where a solid support is used, the phosphorus-atom modified nucleic acid can then be cleaved from the solid support, located at the 5' end. In certain embodiments, the nucleic acids can optionally be left attached on the solid support for purification purposes and then cleaved from the solid support following purification. In one aspect, the synthesis described in Scheme 12 is useful when both of the G1 and G2 position of the chiral auxiliary ligand of Formula A are not hydrogen. The reverse 5' to 3' synthesis can be accomplished using the same starting materials in Scheme 12 in a mechanism analogous to steps in Route A.

Generation of phosphothiotriesters with reversible protecting groups from H-phosphonate [00185] Phosphorothioates can be synthesized in a stereospecific manner from H-phosphonates with retention of configuration at phosphorus atom (J. Org. Chem. 1991, 3861-3869). Also contemplated is the use of this reaction to synthesize phosphorothiotriesters using thiol-containing moiety that also carries bioreversible protecting group, see Scheme 13. Additionally, stereocontrolled solid-phase synthesis of oligonucleoside /f-phosphonates has also been reported (Angew. Chem. Int. Ed. 2009, 48, 496-499) and it is contemplated that this method, combined with alkylation during solid support synthesis, to prepare phosphothiotriesters on support.

Scheme 13

<img file="IN720DEN2012A_D0312.tif" />

i) I₂, ACN: Py (3:2), TBDPSC1 and the respective thiol ii) 3%DCA/DCM

Reaction Conditions and Reagents used in the methods of the invention.

Conditions [00186] The steps of reacting a molecule comprising an achiral //-phosphonate moiety and a nucleoside comprising a 5'-OH moiety to form a condensed intermediate can occur without isolating any intermediates. In some embodiments, the steps of reacting a molecule comprising an achiral //-phosphonate moiety and a nucleoside comprising a 5'-OH moiety to form a condensed intermediate occurs is a one-pot reaction. In an embodiment, a molecule comprising an achiral //-phosphonate moiety, condensing reagent, chiral reagent, and compound comprising a free nucleophilic moiety are added to the reaction mixture at different times. In another embodiment, a molecule comprising an achiral //-phosphonate moiety, condensing reagent, and chiral reagent are present in the same reaction vessel or same pot. In another embodiment, a molecule comprising an achiral //-phosphonate moiety, condensing reagent, chiral reagent, and compound comprising a free nucleophilic moiety are present in the same reaction or same pot. This allows the reaction to be performed without isolation of intermediates and eliminates time-consuming steps, resulting in an economical and efficient synthesis. In specific embodiments, the achiral //-phosphonate, condensing reagent, chiral amino alcohol, 5'-OH nucleoside are present at the same time in a reaction. In a further embodiment, the formation of the chiral intermediate for condensation is formed in situ and is not isolated prior to the condensation reaction. In another embodiment, a molecule comprising an achiral //phosphonate moiety has been activated by reaction with a condensing reagent, chiral reagent in a different reaction vessel from that used when reacting the chiral intermediate with the compound comprising a free 5'OH moiety.

Synthesis on solid support [00187] In some embodiments, the synthesis of the nucleic acid is performed in solution. In other embodiments, the synthesis of the nucleic acid is performed on solid phase. The reactive groups of a solid support may be unprotected or protected. During oligonucleotide synthesis a solid support is treated with various reagents in several synthesis cycles to achieve the stepwise elongation of a growing oligonucleotide chain with individual nucleotide units. The nucleoside unit at the end of the chain which is directly linked to the solid support is termed the first nucleoside as used herein. The first nucleoside is bound to the solid 78 support via a linker moiety, i.e. a diradical with covalent bonds to both the polymer of the solid support and the nucleoside. The linker stays intact during the synthesis cycles performed to assemble the oligonucleotide chain and is cleaved after the chain assembly to liberate the oligonucleotide from the support.

[00188] Solid supports for solid-phase nucleic acid synthesis include the supports described in, e.g., US patents 4,659,774 , 5,141,813, 4,458,066; Caruthers U.S. Pat. Nos. 4,415,732, 4,458,066, 4,500,707, 4,668,777,4,973,679, and 5,132,418; Andrus etal. U.S. Pat. Nos. 5,047,524,5,262,530; and Koster U.S. Pat. Nos. 4,725,677 (reissued as Re34,069). In some embodiments, the solid phase is an organic polymer support. In other embodiments, the solid phase is an inorganic polymer support. In some embodiments, the organic polymer support is polystyrene, aminomethyl polystyrene, a polyethylene glycol-polystyrene graft copolymer, polyacrylamide, polymethacrylate, polyvinylalcohol, highly cross-linked polymer (HCP), or other synthetic polymers, carbohydrates such as cellulose and starch or other polymeric carbohydrates, or other organic polymers and any copolymers, composite materials or combination of the above inorganic or organic materials. In other embodiments, the inorganic polymer support is silica, alumina, controlled polyglass (CPG), which is a silica-gel support, or aminopropyl CPG. Other useful solid supports include fluorous solid supports (see e.g., WO/2005/070859), long chain alkylamine (LCAA) controlled pore glass (CPG) solid supports (see e.g., S. P. Adams, K. S. Kavka, E. J. Wykes, S. B. Holder and G. R. Galluppi, J. Am. Chem. Soc., 1983,105, 661-663; G. R. Gough, M. J. Bruden and P. T. Gilham, Tetrahedron Lett., 1981, 22, 4177-4180). Membrane supports and polymeric membranes (see e.g. Innovation and Perspectives in Solid Phase Synthesis, Peptides, Proteins and Nucleic Acids, ch 21 pp 157-162, 1994, Ed. Roger Epton and U.S. Pat. No. 4,923,901) are also useful for the synthesis of nucleic acids. Once formed, a membrane can be chemically functionalized for use in nucleic acid synthesis. In addition to the attachment of a functional group to the membrane, the use of a linker or spacer group attached to the membrane may be used to minimize steric hindrance between the membrane and the synthesized chain.

[00189] Other suitable solid supports include those generally known in the art to be suitable for use in solid phase methodologies, including, for example, glass sold as PrimerTM 200 support, controlled pore glass (CPG), oxalyl-controlled pore glass (see, e.g., Alul, et al., Nucleic Acids Research, 1991, 19, 1527), TentaGel Support-an aminopolyethyleneglycol derivatized support (see, e.g., Wright, et al., Tetrahedron Lett., 1993,34,3373), and Poros-a copolymer of polystyrene/divinylbenzene.

[00190] Surface activated polymers have been demonstrated for use in synthesis of natural and modified nucleic acids and proteins on several solid supports mediums. The solid support material can be any polymer suitably uniform in porosity, has sufficient amine content, and sufficiently flexible to undergo any attendant manipulations without losing integrity. Examples of suitable selected materials include nylon, polypropylene, polyester, polytetrafluoroethylene, polystyrene, polycarbonate, and nitrocellulose. Other materials can serve as the solid support, depending on the design of the investigator. In consideration of some designs, for example, a coated metal, in particular gold or platinum can be selected (see e.g, US publication No. 20010055761). In one embodiment of oligonucleotide synthesis, for example, a nucleoside 79 is anchored to a solid support which is functionalized with hydroxyl or amino residues. Alternatively, the solid support is derivatized to provide an acid labile trialkoxytrityl group, such as a trimethoxytrityl group (TMT). Without being bound by theory, it is expected that the presence of the trialkoxytrityl protecting group will permit initial detritylation under conditions commonly used on DNA synthesizers. For a faster release of oligonucleotide material in solution with aqueous ammonia, a diglycoate linker is optionally introduced onto the support.

Linking moiety [00191] A linking moiety or linker is optionally used to connect the solid support to the compound comprising a free nucleophilic moiety. Suitable linkers are known such as short molecules which serve to connect a solid support to functional groups (e.g., hydroxyl groups) of initial nucleosides molecules in solid phase synthetic techniques. In some embodiments, the linking moiety is a succinamic acid linker, or a succinate linker (-CO-CH₂-CH₂-CO-), or an oxalyl linker (-CO-CO-). In other embodiments, the linking moiety and the nucleoside are bonded together through an ester bond. In other embodiments, the linking moiety and the nucleoside are bonded together through an amide bond. In further embodiments, the linking moiety connects the nucleoside to another nucleotide or nucleic acid. Suitable linkers are disclosed in, for example, Oligonucleotides And Analogues A Practical Approach, Ekstein, F. Ed., IRL Press, N.Y., 1991, Chapter 1.

[00192] A linker moiety is used to connect the compound comprising a free nucleophilic moiety to another nucleoside, nucleotide, or nucleic acid. In some embodiments, the linking moiety is a phosphodiester linkage. In other embodiments, the linking moiety is an //-phosphonate moiety. In yet other embodiments, the linking moiety is an X-phosphonate moiety.

Solvents for synthesis [00193] Synthesis of the nucleic acids is performed in an aprotic organic solvent. In some embodiments, the solvent is acetonitrile, pyridine, tetrahydrofuran, or dichloromethane. In some embodiments, when the aprotic organic solvent is not basic, a base is present in the reacting step. In some embodiments where a base is present, the base is pyridine, quinoline, or W^V-dimethylaniline. Other examples of bases include pyrrolidine, piperidine, Y-methyl pyrrolidine, pyridine, quinoline, N,Ndimethylaminopyridine (DMAP), or WJV-dimethylaniline. In some embodiments, the aprotic organic solvent is anhydrous. In other embodiments, the anhydrous aprotic organic solvent is freshly distilled. In some embodiments, the freshly distilled anhydrous aprotic organic solvent is pyridine. In other embodiments, the freshly distilled anhydrous aprotic organic solvent is tetrahydrofuran. In other embodiments, the freshly distilled anhydrous aprotic organic solvent is acetonitrile.

Acidification conditions to remove blocking groups.

[00194] Acidification to remove blocking groups is accomplished by a Bronsted acid or Lewis acid. In some embodiments, acidification is used to remove R¹ blocking groups. Useful Bransted acids are carboxylic acids, alkylsulfonic acids, arylsulfonic acids, phosphoric acid and its derivatives, phosphonic acid and its 80 derivatives, alkylphosphonic acids and their derivatives, arylphosphonic acids and their derivatives, phosphinic acid, dialkylphosphinic acids, and diarylphosphinic acids which have a pKa (25 °C in water) value of -0.6 (trifluoroacetic acid) to 4.76 (acetic acid) in an organic solvent or water (in the case of 80% acetic acid). The concentration of the acid (1 to 80%) used in the acidification step depends on the acidity of the acid. Consideration to the acid strength must be taken into account as strong acid conditions will result in depurination/depyrimidination, wherein purinyl or pyrimidinyl bases are cleaved from ribose ring.

RCOOH

R-S-OH

II o

O 0

R¹O-P-OH R¹-P-OH

OR² OR²

O

R¹-P-OH

R²

R = H, alkyl, aryl

R = alkyl, aryl R¹, R² = H, alkyl, aryl

R¹, R² = H, alkyl, aryl

R¹,R² = H, alkyl, aryl [00195] In some embodiments, acidification is accomplished by a Lewis acid in an organic solvent. Useful Lewis acids are ZnX₂ wherein X is Cl, Br, I, or CF3SO3.

[00196] In some embodiments, the acidifying comprises adding an amount of a Bransted or Lewis acid effective to convert the condensed intermediate into the compound of Formula 4 without removing purine moieties from the condensed intermediate.

[00197] Acids that are useful in the acidifying step also include, but are not limited to 10% phosphoric acid in an organic solvent, 10% hydrochloric acid in an organic solvent, 1% trifluoroacetic acid in an organic solvent, 3% dichloroacetic acid in an organic solvent or 80% acetic acid in water. The concentration of any Bransted or Lewis acid used in the process is selected such that the concentration of the acid does not exceed a concentration that causes cleavage of the nucleobase from the sugar moiety.

[00198] In some embodiments, acidification comprises adding 1% trifluoroacetic acid in an organic solvent. In some embodiments, acidification comprises adding about 0.1% to about 8% trifluoroacetic acid in an organic solvent. In other embodiments, acidification comprises adding 3% dichloroacetic acid in an organic solvent. In other embodiments, acidification comprises adding about 0.1% to about 10% dichloroacetic acid in an organic solvent. In yet other embodiments, acidification comprises adding 3% trichloroacetic acid in an organic solvent. In yet other embodiments, acidification comprises adding about 0.1% to about 10% trichloroacetic acid in an organic solvent. In some embodiments, acidification comprises adding 80% acetic acid in water. In some embodiments, acidification comprises adding about 50% to about 90%, or about 50% to about 80%, about 50% to about 70%, about 50% to about 60%, about 70% to about 90% acetic acid in water. In some embodiments, the acidification comprises the further addition of cation scavengers to the acidic solvent. In specific embodiments, the cation scavengers can be triethylsilane or triisopropylsilane. In some embodiments, R¹ is deblocked prior to the step of acidifying the condensed intermediate. In some embodiments, R¹ is deblocked by acidification, which comprises adding 1% trifluoroacetic acid in an organic solvent. In some embodiments, R¹ is deblocked by acidification, which comprises adding 3% dichloroacetic acid in an organic solvent. In some embodiments, R¹ is deblocked by acidification, which comprises adding 3% trichloroacetic acid in an organic solvent.

Removal of blocking moieities or groups [00199] Functional groups such as hydroxyl or amino moieties which are located on nucleobases or sugar moieties are routinely blocked with blocking (protecting) groups (moieties) during synthesis and subsequently deblocked. In general, a blocking group renders a chemical functionality of a molecule inert to specific reaction conditions and can later be removed from such functionality in a molecule without substantially damaging the remainder of the molecule (see e.g., Green and Wuts, Protective Groups in Organic Synthesis, 2nd Ed., John Wiley &amp; Sons, New York, 1991). For example, amino groups can be blocked with nitrogen blocking groups such as phthalimido, 9-fludrenylmethoxycarbonyl (FMOC), triphenylmethylsulfenyl, t-BOC, 4,4’-dimethoxytrityl (DMTr), 4-methoxytrityl (MMTr), 9-phenylxanthin-9yl (Pixyl), trityl (Tr), or 9-(p-methoxyphenyl)xanthin-9-yl (MOX). Carboxyl groups can be protected as acetyl groups. Hydroxy groups can be protected such as tetrahydropyranyl (THP), r-butyldimethylsilyl (TBDMS), l-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp), l-(2-fluorophenyl)-4methoxypiperidin-4-yl (Fpmp), l-(2-chloroethoxy)ethyl, 3-methoxy-l,5-dicarbomethoxypentan-3-yl (MDP), bis(2-acetoxyethoxy)methyl (ACE), triisopropylsilyloxymethyl (TOM), 1 -(2-cyanoethoxy)ethyl (CEE), 2cyanoethoxymethyl (CEM), [4-(7V-dichloroacetylW-methylamino)benzyloxy]methyl, 2-cyanoethyl (CN), pivaloyloxymethyl (PivOM), levunyloxymethyl (ALE). Other representative hydroxyl blocking groups have been described (see e.g., Beaucage et al., Tetrahedron, 1992, 46, 2223). In some embodiments, hydroxyl blocking groups are acid-labile groups, such as the trityl, monomethoxytrityl, dimethoxytrityl, trimethoxytrityl, 9-phenylxanthin-9-yl (Pixyl) and 9-(p-methoxyphenyl)xanthin-9-yl (MOX). Chemical functional groups can also be blocked by including them in a precursor form. Thus an azido group can be considered a blocked form of an amine as the azido group is easily converted to the amine. Further representative protecting groups utilized in nucleic acid synthesis are known (see e.g. Agrawal et al., Protocols for Oligonucleotide Conjugates, Eds., Humana Press, New Jersey, 1994, Vol. 26, pp. 1-72).

[00200] Various methods are known and used for removal of blocking groups from the nucleic acids. In some embodiments, all blocking groups are removed. In other embodiments, the blocking groups are partially removed. In yet other embodiments, reaction conditions can be adjusted to remove blocking groups on certain moieties. In certain embodiments where R² is a blocking group, removal of the blocking group at R² is orthogonal to the removal of the blocking group at R¹. The blocking groups at R¹ and R² remain intact during the synthesis steps and are collectively removed after the chain assembly. In some embodiments, the R² blocking group are removed simultaneously with the cleavage of the nucleic acids from the solid support and with the removal of the nucleobase blocking groups. In specific embodiments, the blocking group at R¹is removed while the blocking groups at R² and nucleobases remain intact Blocking groups at R¹ are cleavable on solid supports with an organic base such as a primary amine, a secondary amine, or a mixture thereof. Deblocking of the R¹ position is commonly referred to as front end deprotection.

[00201] In an embodiment, the nucleobase blocking groups, if present, are cleavable after the assembly of the respective nucleic acid with an acidic reagent. In another embodiment, one or more of the nucleobase 82 blocking groups is cleavable under neither acidic nor basic conditions, e.g. cleavable with fluoride salts or hydrofluoric acid complexes. In yet another embodiment, one or more of the nucleobase blocking groups are cleavable after the assembly of the respective nucleic acid in the presence of base or a basic solvent, and wherein the nucleobase blocking group is stable to the conditions of the front end deprotection step with amines.

[00202] In some embodiments, blocking groups for nucleobases are not required. In other embodiments, blocking groups for nucleobases are required. In yet other embodiments, certain nucleobases require blocking group while other nucleobases do not require blocking groups. In embodiments where the nucleobases are blocked, the blocking groups are either completely or partially removed under conditions appropriate to remove the blocking group at the front end. For example, R¹ can denote OR*, wherein R* is acyl, and Ba denotes guanine blocked with an acyl group including, but not limited to isobutyryl, acetyl or 4(iert-butylphenoxy)acetyl. The acyl groups at RI and Ba will be removed or partially removed during the same deblocking step.

Reagents

Condensing reagent [00203] The condensing reagent (C_R) useful in the methods of the invention has one of the following general formulae:

Z¹ L , 2,3

<img file="IN720DEN2012A_D0313.tif" />

<img file="IN720DEN2012A_D0314.tif" />

or

Z⁷ Q

2®—1^--L

Z⁹

C_r3 C_r4

C_r5 wherein Z¹, Z², Z³, Z⁴, Z⁵, Z⁶, Z⁷, Z⁸, and Z⁹ are independently selected from alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, or heteroaryloxy, or wherein any of Z² and Z³, Z⁵ and Z⁶, Z⁷ and Z⁸, Z⁸ and Z⁹, Z⁹ and Z⁷, or Z⁷ and Z⁸ and Z⁹ are taken together to form a 3 to 20 membered alicyclic or heterocyclic ring; Q’ is a counter anion; and L is a leaving group. [00204] In some embodiments, the counter ion of the condensing reagent Cr is Cl', Br, BF₄‘, PF₆', TfO’, Tf₂N‘, AsF₆’, C1O₄’, or SbF₆‘, wherein Tf is CF₃SO₂. In some embodiments, the leaving group of the condensing reagent C_R is F, Cl, Br, I, 3-nitro-l,2,4-triazole, imidazole, alkyltriazole, tetrazole, pentafluorobenzene, or 1-hydroxybenzotriazole.

[00205] Examples of condensing agents that can be used in the process include, and are not limited to, pentafluorobenzoyl chloride, carbonyldiimidazole (CDI), l-mesitylenesulfonyl-3-nitrotriazole (MSNT), 1ethyl-3-(3’-dimethylaminopropyl)carbodiimide hydrochloride (EDCI-HC1), benzotriazole-1yloxytris(dimethylamino)phosphonium hexafluorophosphate (PyBOP), N,N’-bis(2-oxo-3oxazolidinyl)phosphinic chloride (BopCl), 2-(l/7-7-azabenzotriazol-l-yl)-l,l,3,3-tetramethyluronium hexafluorophosphate (HATU), and O-benzotriazole-ALYA’A⁷ ’-tetramethyluronium hexafluorophosphate (HBTU), DIPCDI; N,2V’-bis(2-oxo-3-oxazolidinyl)phosphinic bromide (BopBr), l,3-dimethyl-2-(3-nitrol,2,4-triazol-l-yl)-2-pyrrolidin-l-yl-l,3,2-diazaphospholidinium hexafluorophosphate (MNTP), 3-nitro83

1,2,4-triazol-1 -yl-tris(pyrrolidin-1 -yl)phosphonium hexafluorophosphate (PyNTP), bromotripyrrolidinophosphonium hexafluorophosphate (PyBrOP); G-(benzotriazol-l-yl)-7V^V^'^tetramethyluronium tetrafluoroborate (TBTU); and tetramethylfluoroformamidinium hexafluorophosphate (TFFH). In certain embodiments, the counter ion of the condensing reagent C_R is CF, Br', BF₄‘, PF₆’, TfO', Tf₂N‘, AsF₆’, C1O₄’, or SbF₆‘, wherein Tf is CF₃SO₂.

[00206] In other embodiments of the invention, the condensing reagent is 1-(2,4,6triisopropylbenzenesulfonyl)-5-(pyridin-2-yl) tetrazolide, pivaloyl chloride, bromotrispyrrolidinophosphonium hexafluorophosphate, NN -bis(2-oxo-3-oxazolidinyl) phosphinic chloride (BopCl), or 2-chloro-5,5-dimethyl-2-oxo-l,3,2-dioxaphosphinane. In one embodiment, the condensing reagent is A\7V’-bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl). Other known condensing reagents have been described (see e.g., WO/2006/066260).

[00207]In other embodiments, the condensing reagent is l,3-dimethyl-2-(3-nitro-l,2,4-triazol-l-yl)-2pyrrolidin-l-yl-l,3,2-diazaphospholidinium hexafluorophosphate (MNTP), or 3-nitro-l,2,4-triazol-l-yltris(pyrrolidin-l-yl)phosphonium hexafluorophosphate (PyNTP).

<td> F~\ r\</td><td></td><td> 0 0</td>

<td> BopCl</td><td> MNTP</td><td> PyNTP</td>

<td> Chiral reagent</td><td></td><td></td>

<td> [00208] In the methods</td><td colspan="2"> of the present invention, chiral reagents are used to confer</td>

stereoselectivity in the production of X-phosphonate linkages. Many different chiral auxiliaries may be used in this process which are compounds of Formula 3-1 where W1 and W2 are any of-0-, -S-, or -NG5-, which are capable of reacting with the /7-phosphonate starting material, a compound of Formula 2 to form the chiral intermediate, as shown in structure III of Schemes 5 and 6.

<img file="IN720DEN2012A_D0315.tif" />

Formula 3-1 [00209] Uj and U₃ are carbon atoms which are bonded to U₂ if present, or to each other if r is 0, via a single, double or triple bond. U₂ is -C-, -CG⁸-, -CG⁸G⁸-, -NG⁸-, -N-, -O-, or -S- where r is an integer of 0 to 5 and no more than two heteroatoms are adjacent. When any one of U2 is C, a triple bond must be formed between a second instance of U2, which is C, or to one of Ui or U3. Similarly, when any one of U2 is CG⁸, a double bond is formed between a second instance of U2 which is -CG⁸- or -N-, or to one of Ui or U₃.

[00210] For example, in some embodiments, -Ui-(U₂)_r-U₃- is -CG³G⁴-CG'G²-. In some embodiments, -Ur (U2)r-U3- is -CG³= CG¹-. In some embodiments, -Ur(U2)_r-U₃- is -C=C-. In some embodiments, -U_r(U₂\

U₃- is -CG³=C G’-CG’G²-. In some embodiments, -Ur(U2)r-U3- is -CG³G⁴-O-CG¹G²-. In some embodiments, -Ur(U₂)_r-U₃- is -CG³G⁴-NG⁸-CG*G²-. In some embodiments, -U,-(U2)r-U3- is -CG³G⁴-N-CG². In some embodiments, -Ur(U₂)_r-U₃- is -CG³G⁴-N=C G’-CG’G²-.

[00211] G¹, G², G³, G⁴, G⁵, and G⁸are independently hydrogen, alkyl, aralkyl, cycloalkyl, cycloalkylalkyl, heterocyclyl, hetaryl, or aryl, or two of G¹, G², G³, G⁴, and G⁵ are G⁶ taken together form a saturated, partially unsaturated or unsaturated carbocyclic or heteroatom-containing ring of up to about 20 ring atoms which is monocyclic or polycyclic, and is fused or unfused. In some embodiments, the ring so formed is substituted by oxo, thioxo, alkyl, alkenyl, alkynyl, heteroaryl, or aryl moieties. In some embodiments, when the ring formed by taking two G⁶ together is substituted, it is substituted by a moiety which is bulky enough to confer stereoselectivity during the reaction.

[00212] For example, in some embodiments, the ring formed by taking two of G⁶ together is cyclopentyl, pyrrolyl, cyclopropyl, cyclohexenyl, cyclopentenyl, tetrahydropyranyl, or piperazinyl.

[00213] In some embodiments of the invention, the chiral reagent is a compound of Formula 3.

H—Wi W₂—H

G³ G²

Formula 3 [00214] In some embodiments of Formula 3, Wi and W₂ are independently -NG⁵-, -O-, or -S-; G¹, G², G³, G⁴, and G⁵ are independently hydrogen, alkyl, aralkyl, cycloalkyl, cycloalkylalkyl, heterocyclyl, hetaryl, or aryl, or two of G¹, G², G³, G⁴, and G⁵ are G⁶ taken together form a saturated, partially unsaturated or unsaturated carbocyclic or heteroatom-containing ring of up to about 20 ring atoms which is monocyclic or polycyclic, fused or unfused, and no more than four of G¹, G², G³, G⁴, and G⁵ are G⁶. Similarly to the compounds of Formula 3’, any of G¹, G², G³, G⁴, or G⁵ are substituted by oxo, thioxo, alkyl, alkenyl, alkynyl, heteroaryl, or aryl moieties. In some embodiments, such substitution induces stereoselectivity in Xphosphonate production.

[00215] In some embodiments of the invention, the chiral reagent has one of the following Formulae:

<td> g⁵-nh oh G⁴G³ G²</td><td> g⁵-nh sh g⁴H'_G' G³ G²</td><td> g⁵-nh hn-g° ^g14^g'</td><td> HS OH ^G4O'^G1</td><td> HO OH G^W'Ql G³ G²</td><td> HS SH G⁴ %SG¹ G³ G²</td>

<td> Formulae 3-A</td><td> 3-B</td><td> 3-C</td><td> 3-D</td><td> 3-E</td><td> 3-F</td>

[00216] In some embodiments, the chiral reagent is an aminoalcohol. In some other embodiments, the chiral reagent is an aminothiol. In yet other embodiments, the chiral reagent is an aminophenol. In some embodiments, the chiral reagent is (5)- and (5)-2-methyl amino-1 -phenylethanol, (11?, 25)-ephedrine, or (15, 25)-2-methy lamino-1,2-dipheny lethanol.

{00217] In other embodiments of the invention the chiral reagent is a compound of one of the following formulae:

<img file="IN720DEN2012A_D0316.tif" />

Formula Ο

<img file="IN720DEN2012A_D0317.tif" />

Formula P Formula Q

<img file="IN720DEN2012A_D0318.tif" />

Formula R [00218] The choice of chiral reagent, for example, the isomer represented by Formula O or its stereoisomer, Formula P, permits the specific control of the chirality at phosphorus. Thus either a ΛΡ or SP configuration can be selected in each synthesis cycle, permitting control of the overall three dimensional structure of the nucleic acid product. In some embodiments of the invention, a nucleic acid product has all RP stereocenters. In some embodiments of the invention, a nucleic acid product has all SP stereocenters. In some embodiments, the selection of RP and SP centers is made to confer a specific three dimensional superstructure to the nucleic acid chain.

Stereochemistry of Oligonucleoside Phosphorothioate Linkages [00219] Oligonucleoside phosphorothioates have shown therapeutic potential (Stein et al.,

Science (1993), 261:1004-12; Agrawal et al., Antisence Res. and Dev. (1992), 2:261-66; Bayever et al., Antisense Res. and Dev. (1993), 3:383-390). Oligonucleoside phosphorothioates prepared without regard to the sterochemistry of the phosphorothioate exist as a mixture of 2n diastereomers, where n is the number of intemucleotide phosphorothioates linkages. The chemical and biological properties of these diastereomeric phosphorothioates can be distinct. For example, Wada et al (Nucleic Acids Symposium Series No. 51 p. 119-120; doi:10.1093/nass/nrm060) found that stereodefined-(Rp)-(Ups)9U/(Ap)9A duplex showed a higher Tm value than that of natural-(Up)9U/(Ap)9A and stereodefined-(Sp)-(Ups)9U did not form a duplex. In another example, in a study by Tang et al., (Nucleosides Nucleotides (1995), 14:985-990) stereopure Rpoligodeoxyribonucleoside phosphorothioates were found to possess lower stability to nucleases endogenous to human serum that the parent oligodeoxyribonucleoside phosphorothioates with undefined phosphorous chirality.

Nucieobases and Modified Nucleobases [00220] The nucleobase Ba in Formula 1 is a natural nucleobase or a modified nucleobase derived from natural nucleobases. Examples include, but are not limited to, uracil, thymine, adenine, cytosine, and guanine having their respective amino groups protected by acyl protecting groups, 2-fluorouracil, 2fluorocytosine, 5-bromouracil, 5-iodouracil, 2,6-diaminopurine, azacytosine, pyrimidine analogs such as pseudoisocytosine and pseudouracil and other modified nucleobases such as 8-substituted purines, xanthine, or hypoxanthine (the latter two being the natural degradation products). The modified nucleobases disclosed in Chiu and Rana, RNA, 2003, 9, 1034-1048, Limbach et al. Nucleic Acids Research, 1994, 22, 2183-2196 and Revankar and Rao, Comprehensive Natural Products Chemistry, vol. 7, 313, are also contemplated as Ba moieties of Formula 1.

[00221] Compounds represented by the following general formulae are also contemplated as modified nucleobases:

<img file="IN720DEN2012A_D0319.tif" />

<img file="IN720DEN2012A_D0320.tif" />

<img file="IN720DEN2012A_D0321.tif" />

<img file="IN720DEN2012A_D0322.tif" />

[00222] In the formulae above, R⁸ is a linear or branched alkyl, aryl, aralkyl, or aryloxylalkyl group having 1 to 15 carbon atoms, including, by way of example only, a methyl, isopropyl, phenyl, benzyl, or phenoxymethyl group; and each of R⁹ and R¹⁰ represents a linear or branched alkyl group having 1 to 4 carbon atoms.

[00223] Modified nucleobases also include expanded-size nucleobases in which one or more benzene rings has been added. Nucleic base replacements described in the Glen Research catalog (www.glenresearch.com); Krueger AT et al, Acc. Chem. Res., 2007, 40, 141-150; Kool, ET, Acc. Chem. Res., 2002, 35, 936-943; Benner S.A., et al., Nat. Rev. Genet., 2005, 6, 553-543; Romesberg, F.E., et al., Curr. Opin. Chem. Biol., 2003, 7, 723-733; Hirao, I., Curr. Opin. Chem. Biol., 2006, 10, 622-627, are contemplated as useful for the synthesis of the nucleic acids described herein. Some examples of these expanded-size nucleobases are shown below:

<img file="IN720DEN2012A_D0323.tif" />

[00224] Herein, modified nucleobases also encompass structures that are not considered nucleobases but are other moieties such as, but not limited to, corrin- or porphyrin-derived rings. Porphyrin-derived base replacements have been described in Morales-Rojas, H and Kool, ET, Org. Lett., 2002, 4, 4377-4380. Shown below is an example of a porphyrin-derived ring which can be used as a base replacement:

<img file="IN720DEN2012A_D0324.tif" />

<img file="IN720DEN2012A_D0325.tif" />

[00226] Modified nucleobases which are fluorescent are also contemplated. Non-limiting examples of these base replacements include phenanthrene, pyrene, stillbene, isoxanthine, isozanthopterin, terphenyl, terthiophene, benzoterthiophene, coumarin, lumazine, tethered stillbene, benzo-uracil, and naphtho-uracil, as shown below:

<img file="IN720DEN2012A_D0326.tif" />

<img file="IN720DEN2012A_D0327.tif" />

[00227] The modified nucleobases can be unsubstituted or contain further substitutions such as heteroatoms, alkyl groups, or linking moieties connected to fluorescent moieties, biotin or avidin moieties, or other protein or peptides. Modified nucleobases also include certain 'universal bases' that are not nucleobases in the most classical sense, but function similarly to nucleobases. One representative example of such a universal base is 3-nitropyrrole.

[00228] In addition to nucleosides of structure IV or IX, other nucleosides can also be used in the process disclosed herein and include nucleosides that incorporate modified nucleobases, or nucleobases covalently bound to modified sugars. Some examples of nucleosides that incorporate modified nucleobases include 4acetylcytidine; 5-(carboxyhydroxylmethyl)uridine; 2'-O-methylcytidine; 5-carboxymethylaminomethyl-2thiouridine; 5-carboxymethylaminomethyluridine; dihydrouridine; 2'-O-methylpseudouridine; beta,Dgalactosylqueosine; 2'-0-methylguanosine; V6-isopentenyladenosine; 1-methyladenosine; 1methylpseudouridine; 1-methylguanosine; 1-methylinosine; 2,2-dimethylguanosine; 2-methyladenosine; 2methylguanosine; 7V7-methylguanosine; 3-methyl-cytidine; 5-methylcytidine; M-methyladenosine; Ίmethylguanosine; 5-methylaminoethyluridine; 5-methoxyaminomethyl-2-thiouridine; beta,Dmannosylqueosine; 5-methoxycarbonylmethyluridine; 5-methoxyuridine; 2-methylthio-7V6isopentenyladenosine; V-((9-beta,D-ribofuranosyl-2-methylthiopurine-6-yl)carbamoyl)threonine; N-((9beta,D-ribofuranosylpurine-6-yl)-V-methylcarbamoyl)threonine; uridine-5-oxyacetic acid methylester; uridine-5-oxyacetic acid (v); pseudouridine; queosine; 2-thiocytidine; 5-methyl-2-thiouridine; 2-thiouridine; 4-thiouridine; 5-methyluridine; 2'-O-methyl-5-methyluridine; and 2'-O-methyluridine.

[00229] In some embodiments, nucleosides include 6'-modified bicyclic nucleoside analogs that have either (R) or (S)-chirality at the 6'-position and include the analogs described in US Patent No. 7,399,845. In other embodiments, nucleosides include 5'-modified bicyclic nucleoside analogs that have either (R) or (flchirality at the 5'-position and include the analogs described in US Patent Application Publication No. 20070287831.

[00230] In some embodiments, the nucleobases or modified nucleobases comprises biomolecule binding moieties such as antibodies, antibody fragments, biotin, avidin, streptavidin, receptor ligands, or chelating moieties. In other embodiments, Ba is 5-bromouracil, 5-iodouracil, or 2,6-diaminopurine. In yet other embodiments, Ba is modified by substitution with a fluorescent or biomolecule binding moiety. In some embodiments, the substituent on Ba is a fluorescent moiety. In other embodiments, the substituent on Ba is biotin or avidin.

Modified sugars of the nucleotide/nucleoside.

[00231] The most common naturally occurring nucleotides are ribose sugars linked to the nucleobases adenosine (A), cytosine (C), guanine (G), and thymine (T) or uracil (U). Also contemplated are modified nucleotides wherein the phosphate group or the modified phosphorous atom moieties in the nucleotides can be linked to various positions of the sugar or modified sugar. As non-limiting examples, the phosphate group or the modified phosphorous-atom moiety can be linked to the 2', 3', 4' or 5' hydroxyl moiety of a sugar or modified sugar. Nucleotides that incorporate the modified nucleobases described above can also be used in the process disclosed herein. In some embodiments, nucleotides or modified nucleotides comprising an unprotected -OH moiety are used in the process disclosed herein.

[00232] In addition to the ribose moiety described in Schemes l-4b, other modified sugars can also be incorporated in the nucleic acids disclosed herein. In some embodiments, the modified sugars contain one or 89 more substituents at the 2' position including one of the following: F; CF₃, CN, N₃, NO, NO₂, 0-, S-, or Nalkyl ; 0-, S-, or N-alkenyl; 0-, S- or N-alkynyl; or O-alkyl-O-alkyl, O-alkyl-N-alkyl or N-alkyl-O-alkyl wherein the alkyl, alkenyl and alkynyl may be substituted or unsubstituted Ci-Cio alkyl or C₂-Ci₀ alkenyl and alkynyl. Examples of substituents include, and are not limited to, O(CH₂)_nOCH₃, and O(CH₂)_nNH₂, wherein n is from 1 to about 10, MOE, DMAOE, DMAEOE. Also contemplated herein are modified sugars described in WO 2001/088198; and Martin et al., Helv. Chim. Acta, 1995, 78, 486-504. In some embodiments, modified sugars comprise substituted silyl groups, an RNA cleaving group, a reporter group, a fluorescent label, an intercalator, a group for improving the pharmacokinetic properties of a nucleic acid, or a group for improving the pharmacodynamic properties of a nucleic acid, and other substituents having similar properties. The modifications may be made at the at the 2', 3', 4', 5', or 6' positions of the sugar or modified sugar, including the 3' position of the sugar on the 3'-terminal nucleotide or in the 5' position of the 5'terminal nucleotide.

[00233] Modified sugars also include sugar mimetics such as cyclobutyl or cyclopentyl moieties in place of the pentofuranosyl sugar. Representative United States patents that teach the preparation of such modified sugar structures include, but are not limited to, US Patent Nos.: 4,981,957; 5,118,800; 5,319,080 ; and

5,359,044. Some modified sugars that are contemplated include:

<img file="IN720DEN2012A_D0328.tif" />

Q = Me, Et, /-Pr.

[00234] Other non-limiting examples of modified sugars include glycerol, which form glycerol nucleic acid (GNA) analogues. One example of a GN A analogue is shown below and is described in Zhang, R et al., J. Am. Chem. Soc., 2008,130, 5846-5847; Zhang L, et al., J. Am. Chem. Soc., 2005,127, 4174-4175 and Tsai

CH et al., PNAS, 2007, 14598-14603:

<img file="IN720DEN2012A_D0329.tif" />

[00235] wherein X is as defined herein. Another example of a GNA derived analogue, flexible nucleic acid (FNA) based on the mixed acetal aminal of formyl glycerol, is described in Joyce GF et al., PNAS, 1987,84, 4398-4402 and Heuberger BD and Switzer C, J. Am. Chem. Soc., 2008,130, 412-413, and is shown below:

«ΑΛΛΑ

Ο ι

<img file="IN720DEN2012A_D0330.tif" />

Ο

<img file="IN720DEN2012A_D0331.tif" />

ο

JL [00236] Other non-limiting examples of modified sugars include hexopyranosyl (6’ to 4’), pentopyranosyl (4’ to 2’), pentopyranosyl (4’ to 3’), or tetrofuranosyl (3’ to 2’) sugars.

Hexopyranosyl (6’ to 4’) sugars contemplated include:

<img file="IN720DEN2012A_D0332.tif" />

<img file="IN720DEN2012A_D0333.tif" />

[00237]Pentopyranosyl (4’ to 2’) sugars contemplated include:

<img file="IN720DEN2012A_D0334.tif" />

<img file="IN720DEN2012A_D0335.tif" />

«/vw [00238] Pentopyranosyl (4’ to 3’) sugars contemplated include:

<img file="IN720DEN2012A_D0336.tif" />

ywv [00239]Tetrofuranosyl (3’ to 2’) sugars contemplated include:

X

<img file="IN720DEN2012A_D0337.tif" />

<img file="IN720DEN2012A_D0338.tif" />

<img file="IN720DEN2012A_D0339.tif" />

[00240] Other modified sugars contemplated include:

<img file="IN720DEN2012A_D0340.tif" />

[00241 [Further contemplated are the sugar mimetics illustrated below wherein X is selected from S, Se, CH₂, N-Me, N-EtorN-iPr.

<img file="IN720DEN2012A_D0341.tif" />

<img file="IN720DEN2012A_D0342.tif" />

<img file="IN720DEN2012A_D0343.tif" />

λ/w ww

<img file="IN720DEN2012A_D0344.tif" />

<img file="IN720DEN2012A_D0345.tif" />

[00242] The modified sugars and sugar mimetics can be prepared by methods known in the art, including, but not limited to: A. Eschenmoser, Science (1999), 284:2118; M. Bohringer et al, Helv. Chim. Acta (1992), 75:1416-1477; M. Egli et al, J. Am. Chem. Soc. (2006), 128(33): 10847-56; A. Eschenmoser in Chemical Synthesis: Gnosis to Prognosis, C. Chatgilialoglu and V. Sniekus, Ed., (Kluwer Academic, Netherlands, 1996), p.293; K.-U. Schoning et al, Science (2000), 290:1347-1351; A. Eschenmoser et al, Helv. Chim. Acta (1992), 75:218; J. Hunziker et al, Helv. Chim. Acta (1993), 76:259; G. Otting et al, Helv. Chim. Acta (1993), 76:2701; K. Groebke et al, Helv. Chim. Acta (1998), 81:375; and A. Eschenmoser, Science (1999), 284:2118.

Blocking groups [00243] In the reactions described, it is necessary in certain embodiments to protect reactive functional groups, for example hydroxy, amino, thiol or carboxy groups, where these are desired in the final product, to avoid their unwanted participation in the reactions. Protecting groups are used to block some or all reactive moieties and prevent such groups from participating in chemical reactions until the protective group is removed. In one embodiment, each protective group is removable by a different means. Protective groups that are cleaved under totally disparate reaction conditions fulfill the requirement of differential removal. In some embodiments, protective groups are removed by acid, base, and/or hydrogenolysis. Groups such as trityl, dimethoxytrityl, acetal and t-butyldimethylsilyl are acid labile and are used in certain embodiments to protect carboxy and hydroxy reactive moieties in the presence of amino groups protected with Cbz groups, which are removable by hydrogenolysis, and/or Fmoc groups, which are base labile. In other embodiments, carboxylic acid and hydroxy reactive moieties are blocked with base labile groups such as, but not limited to, methyl, ethyl, and acetyl in the presence of amines blocked with acid labile groups such as r-butylcarbamate or with carbamates that are both acid and base stable but hydrolytically removable.

[00244] In another embodiment, hydroxy reactive moieties are blocked with hydrolytically removable protective groups such as the benzyl group, while amine groups capable of hydrogen bonding with acids are blocked with base labile groups such as Fmoc. In another embodiment, carboxylic acid reactive moieties are protected by conversion to simple ester compounds, or they are, in yet another embodiment, blocked with oxidatively-removable protective groups such as 2,4-dimethoxybenzyl, while co-existing amino groups are blocked with fluoride labile silyl or carbamate blocking groups.

[00245] Allyl blocking groups are useful in the presence of acid- and base- protecting groups since the former are stable and can be subsequently removed by metal or pi-acid catalysts. For example, an allylblocked hydroxy groups can be deprotected with a Pd(0)-catalyzed reaction in the presence of acid labile tbutylcarbamate or base-labile acetate amine protecting groups. Yet another form of protecting group is a resin to which a compound or intermediate is attached. As long as the residue is attached to the resin, that functional group is blocked and cannot react. Once released from the resin, the functional group is available to react.

(00246] Typically blocking/protecting groups useful in the synthesis of the compounds described herein are, by way of example only:

<img file="IN720DEN2012A_D0346.tif" />

allvl

<img file="IN720DEN2012A_D0347.tif" />

<img file="IN720DEN2012A_D0348.tif" />

M£

H3C''-'·'/

Et

Bn Cbz &lt;, H₃C_{x z}ch₃ (HjCHC-^T (H₃C)₃C'^S,'-'y·'

O alloc

<img file="IN720DEN2012A_D0349.tif" />

t-butvl TBDMS

<img file="IN720DEN2012A_D0350.tif" />

<img file="IN720DEN2012A_D0351.tif" />

acetyl Fmoc [00247] Representative protecting groups useful to protect nucleotides during synthesis include base labile protecting groups and acid labile protecting groups. Base labile protecting groups are used to protect the exocyclic amino groups of the heterocyclic nucleobases. This type of protection is generally achieved by acylation. Three commonly used acylating groups for this purpose are benzoyl chloride, phenoxyacetic anhydride, and isobutyryl chloride. These protecting groups are stable to the reaction conditions used during nucleic acid synthesis and are cleaved at approximately equal rates during the base treatment at the end of synthesis.

[00248] In some embodiments, the 5'-protecting group is trityl, monomethoxy trityl, dimethoxytrityl, trimethoxytrityl, 2-chlorotrityl, DATE, TBTr, 9-phenylxanthine-9-yl (Pixyl), or 9-(pmethoxyphenyl)xanthine-9-yl (MOX).

[00249] In some embodiments, thiol moieties are incorporated in the compounds of Formula 1,2,4, or 5 and are protected. In some embodiments, the protecting groups include, but are not limited to, pixyl, trityl, benzyl, p-methoxybenzyl (PMB), or teri-butyl (i-Bu).

[00250] Other protecting groups, plus a detailed description of techniques applicable to the creation of protecting groups and their removal are described in Greene and Wuts, Protective Groups in Organic Synthesis, 3rd Ed., John Wiley &amp; Sons, New York, NY, 1999, and Kocienski, Protective Groups, Thieme Verlag, New York, NY, 1994, which are incorporated herein by reference for such disclosure.

METHODS OF USE OF THE NUCLEIC ACID PRODRUGS COMPRISING A CHIRAL XPHOSPHONATE MOIETY [00251J The stereodefined oligonucleotide prodrug comprising a chiral phosphorous or phosphorothioate moiety which are obtained by the methods of the invention are useful in a number of areas for applications due to a combination of stability, defined chirality and ease of synthesis. Broadly, the compounds synthesized by this method are useful as therapeutics, diagnostic probes and reagents, synthetic tools for producing other oligonucleotide products, and nanostructure materials suitable for a variety of new materials and computing applications.

[00252] The stereodefined oligonucleotide prodrug of the invention have improved serum stability over that of natural DNA/RNA equivalents, and in particular, stereodefined oligonucleotide prodrug of the class of phosphorothioates. Further, the SP isomer is more stable than the RP isomer. In some embodiments, the level of serum stability is modulated by the introduction of either all SP centers or SP centers at selected positions to confer resistance to degradation. In other embodiments, introduction of selectable RP and/or S_Pstereocenters can provide for specific base pairing association with an endogenous or exogenous target thus protecting the target from metabolism or enhancing a particular biological reaction.

[00253] RNase H activation is also modulated by the presence of the stereocontrolled phosphorothioate nucleic acid analogs, with natural DNA/RNA being more susceptible than the RP stereoisomer which in turn is more susceptible than the corresponding S_P isomer.

[00254] Improved duplex stability towards RNA is seen with RP phosphorothioate oligonucleotides having greater duplex stability than corresponding SP oligonucleotides which in turn demonstrates higher stability than that of natural DNA/RNA. Improved duplex stability towards DNA is seen with SP having greater duplex stability than 7?P which has more stability than that of natural DNA/RNA. (P. Guga, Curr. Top Med. Chem., 2007,7,695-713).

[00255] These molecules may be useful as therapeutic agents, in a number of particular applications. They can be incorporated into oligonucleotides which also contain the standard DNA/RNA nucleosides, or they may be synthesized as entire sequences of the stereocontrolled oligonucleotides of the invention. Some categories of therapeutic agents include but are not limited to antisense oligonucleotides, antigene oligonucleotides which form triple helix with targeted sequences to repress transcription of undesired genes and modulate protein expression and/or activity, decoy oligonucleotides, DNA vaccines, aptamers, ribozymes, deoxyribozymes (DNAzymes or DNA enzymes), siRNAs, microRNAs, ncRNAs (non-coding RNAs), and P-modified prodrugs. Modulation encompasses indirectly or directly increasing or decreasing the activity of a protein or inhibition or promotion of the expression of a protein. These nucleic acid compounds can be used to control cell proliferation, viral replication, or any other cell signaling process.

[00256] In one example, the field of siRNA therapeutics has a need for oligonucleotide species that can afford increased stability against RNase activity, in order to improve the duration of action over that seen with siRNA composed of natural nucleosides. Additionally, A-form helix formation appears to be more indicative of success at entering RNAi than the presence of specific native elements on the oligonucleotide. Both of these requirements can be afforded by the use of the stereocontrolled oligonucleotides of the invention may provide enhanced stability (Y-L Chiu, T.M. Rana RNA, 2003,9,1034-1048).

Methods of Treatment [00257] The nucleic acids described herein are useful as therapeutic agents against various disease states, including use as antiviral agents. The nucleic acids can be used as agents for treatment of diseases through modulation of DNA and/or RNA activity. In some embodiments, the nucleic acids can be used for inhibiting specific gene expression. For example, the nucleic acids can be complementary to a specific target messenger RNA (mRNA) sequence. They can be used to inhibit viral replication of myriad viruses. Exemplary virus families include orthomyxoviruses, pox viruses, herpes viruses, papilloma viruses, picomaviruses, flaviviruses, retroviruses, hepatitis viruses, paramyxoviruses, reoviruses, parvoviruses, filoviruses, coronaviruses, arenaviruses, rhabdoviruses and adenoviruses. Additional virus families are known and are also contemplated herein. Other examples include uses as antisense compounds against HIV RNA or other retroviral RNA or for hybridizing to HIV mRNA encoding the tat protein, or to the TAR region of HIV mRNA. In some embodiments, the nucleic acids mimic the secondary structure of the TAR region of HIV mRNA, and by doing so bind the tat protein. In an embodiment, the nucleic acids is used to inhibit expression of a target protein by contacting a cell with a compound of Formula 1 wherein the expression of other proteins in the cell are not inhibited or are minimally inhibited. In some embodiment, target protein inhibition occurs in vivo in a mammal. In other embodiments, a therapeutically effective amount of a compound of Formula 1 is administered for inhibiting the expression of a target protein.

[00258] Other examples of proteins where expression can be modulated include Jim N-terminal kinase (JNK) proteins, diacylglycerol acyltransferase I, apolipoprotein B, glucagon receptor, Aurora B, acyl CoA cholesterol acyltransferase-2, c-reactive protein, STAT (signal transducers and activators of transcription) family of proteins, and MDR P-glycoprotein. The nucleic acids can be used to inhibit protein phosphatase IB (PTP1B) expression, RNA-dependent RNA viral polymerase. The nucleic acids can be used to induce events such as apoptosis in cancer cells or to make a cell more susceptible to apoptosis. The nucleic acids can be used to modulate activities of proteins. For example, it can help modulate RNase H activity targeting multidrug resistance (MDR) RNA molecules.

[00259] In another aspect, the present invention provides methods of treating a disease mediated by undesired gene expression in a subject (e.g., mammals, such as humans) in need of such treatment. By diseases is meant diseases, or disease symptoms. The method includes administering to the subject an effective amount of a non-racemic pro-oligonucleotide of the present invention.

(00260] Examples of diseases mediated by undesired gene expression include cancer (e.g., leukemia, tumors, and metastases), allergy, asthma, obesity, inflammation (e.g., inflammatory diseases such as inflammatory airways disease), hypercholesterolemia, hematological disorders, severe acute respiratory syndrome (SARS), obstructive airways disease, asthma, autoimmune diseases, retroviral diseases such as AIDS or HIV, other viral infections, intrauterine infections, metabolic diseases, infection (e.g., bacterial, viral, yeast, fungal), CNS diseases, brain tumors, degenerative neural diseases, cardiovascular diseases, and diseases associated with angiogenesis, neovascularization, and vasculogenesis.

[00261] In an exemplary embodiment, the compounds are useful for treating cancer, including pancreatic cancer, and other diseases or disorders involving abnormal cell proliferation.

[00262] Located in the upper abdomen (in the retroperitoneum), the pancreas is a dual-function gland of the digestive and endocrine system. In certain instances, the pancreas functions as an endocrine gland (e.g., producing several important hormones). In certain instances, the pancreas functions as an exocrine gland (e.g., secreting fluids containing digestive enzymes that pass to the small intestine).

[00263] Pancreatic cancer is the fourth most common cause of cancer death in the US (after lung, colon and breast), comprising 6% of all cancer-related deaths. In 2008, an estimated 37,680 new cases of pancreatic cancer will have been diagnosed in the US, with 34,290 deaths. Incidence of the disease, rises linearly after age 50, with the only definitive risk factor being cigarette smoking (smokers are four times more likely to develop the disease than non-smokers). Invasive pancreatic cancer is almost always fatal. The collective median survival time of all patients is 4-6 months. Relative 1-year survival is 24%; the overall 5-year survival rate &lt; 5%.

(00264] Pancreatic cancer is asymptomatic in its early stage and often remains undiagnosed for several months (less than one third of patients being diagnosed within 2 months of the onset symptoms). In certain instances, the delayed diagnosis results in (either partially or fully) metastasis of the cancerous cells to the liver or lymph nodes.

[00265] Currently, surgery (resectioning of the pancreas) is the primary and only curative therapy for pancreatic cancer. However, only 15-25% of tumors are resectable at the time of diagnosis and only 10-20% of patients undergoing surgery survive more than two years. Once tumor infiltration occurs and other tissues have been affected, surgery is no longer possible.

[00266] In certain instances, diabetes mellitus or pancreatitis predisposes an individual to develop a proliferative disorder of a plurality of pancreatic cells. In certain instances, individuals are at an increased risk of developing a proliferative disorder of a plurality of pancreatic cells due to a hereditary syndrome selected from the group consisting of hereditary nonpolyposis colorectal cancer (HNPCC) and familial adenomatous polyposis (FAP). In certain instances, individuals are at an increased risk of developing a proliferative disorder of a plurality of pancreatic cells due to a mutation in a gene selected from the group consisting of MSH2, MSH6, MLH1, and APC.

[00267] Ideally, effective treatment of pancreatic cancer should (i) control the primary tumor mass, both initially and subsequently, and (ii) treat the metastatic tumor cells. Chemoprevention (the administration of agents such as drugs, biologies, nutrients and the like) slows the progression of, reverses, or inhibits carcinogenesis, thereby lowering the risk of developing invasive or clinically significant disease.

[00268] Disclosed herein, in certain embodiments, is a method of treating pancreatic cancer. As used herein, “pancreatic cancer” includes forms of cancer of the pancreas. In some embodiments, the pancreatic cancer is metastatic pancreatic cancer. In some embodiments, the pancreatic cancer is a carcinoma, sarcoma, cancer, or combinations thereof. In some embodiments, a pancreatic cancer to be treated includes sporadic and hereditary pancreatic cancers. In some embodiments, the pancreatic cancer is duct cell carcinoma, acinar cell carcinoma, papillary mucinous carcinoma, signet ring carcinoma, adenosquamous carcinoma, undifferentiated carcinoma, mucinous carcinoma, giant cell carcinoma, small cell carcinoma, cystcancer, serous cystcancer, mucinous cystcancer, unclassified pancreatic cancer, pancreatoblastoma, or combinations thereof.

[00269] In some embodiments, an individual in need of treatment for pancreatic cancer presents with a localized tumor of the pancreas. In some embodiments, an individual in need of treatment for pancreatic cancer presents with a negative regional lymph node biopsy. In some embodiments, an individual in need of treatment for pancreatic cancer presents with a positive regional lymph node biopsy. In some embodiments, an individual in need of treatment for pancreatic cancer presents with a nodal negative pancreatic tumor (e.g., node-negative). In some embodiments, an individual in need of treatment for pancreatic cancer presents with a nodal positive tumor (e.g., node-positive).

[00270] In some embodiments, the pancreatic cancer in an individual in need of treatment for pancreatic cancer has metastasized to other locations in the body. In some embodiments, the pancreatic cancer has metastasized to a location selected from the group consisting of lymph node, stomach, bile duct, liver, bone, ovary, peritoneum and brain.

[00271] In some embodiments, cancer cells or precancerous cells are identified by histological typing or grading of a tissue sample (e.g., a biopsy sample). In some embodiments, cancer cells or precancerous cells are identified through the use of appropriate molecular markers.

[00272] In some embodiments, the pancreatic cancer in an individual in need of treatment for pancreatic cancer is staged according to the American Joint Committee on Cancer (AJCC) TNM classification system, where the tumor (T) has been assigned a stage of Tx, Tl, T2, T3, T4; and where the regional lymph nodes (N) have been assigned a stage of NX, NO, Nl; and where distant metastasis (M) has been assigned a stage of MX, MO, or Ml. In some embodiments, the pancreatic cancer in an individual in need of treatment for pancreatic cancer is staged as Stage 0, I, IA, IB, II, IIA, IIB, III, and IV pancreatic cancer. In some embodiments, the pancreatic cancer in an individual in need of treatment for pancreatic cancer is staged as Grade GX (e.g., grade cannot be assessed), Grade 1, Grade 2, Grade 3 or Grade 4.

[00273] More specific examples of cancers treated with the compounds of the present invention include breast cancer, lung cancer, melanoma, colorectal cancer, bladder cancer, ovarian cancer, prostate cancer, renal cancer, squamous cell cancer, glioblastoma, Kaposi's sarcoma, multiple myeloma, and leukemia. Evaluation and Treatment of Cancer [00274] The term “tumor cell antigen” is defined herein as an antigen that is present in higher quantities on a tumor cell or in body fluids than unrelated tumor cells, normal cells, or in normal body fluid. The antigen presence may be tested by any number of assays known to those skilled in the art and include without limitation negative and/or positive selection with antibodies, such as an ELISA assay, a radioimmunoassay, or by Western Blot.

[00275] “Apoptosis inducing agent” is defined herein to induce apoptosis/programmed cell death, and include, for example, anticancer agents and treatments wherein cells (e.g., tumor cells) are induced to undergo programmed cell death. Exemplary apoptosis inducing agents are described in more detail below.

[00276] The terms “apoptosis” or “programmed cell death,” refers to the physiological process by which unwanted or useless cells are eliminated during development and other normal biological processes. Apoptosis is a mode of cell death that occurs under normal physiological conditions and the cell is an active participant in its own demise (“cellular suicide”). It is most often found during normal cell turnover and tissue homeostasis, embryogenesis, induction and maintenance of immune tolerance, development of the nervous system and endocrine-dependent tissue atrophy. Cells undergoing apoptosis show characteristic morphological and biochemical features. These features include chromatin aggregation, nuclear and cytoplasmic condensation, partition of cytoplasm and nucleus into membrane bound vesicles (apoptotic bodies), which contain ribosomes, morphologically intact mitochondria and nuclear material. In vivo, these apoptotic bodies are rapidly recognized and phagocytized by macrophages, dendritic cells or adjacent epithelial cells. Due to this efficient mechanism for the removal of apoptotic cells in vivo no inflammatory response is elicited. In vitro, the apoptotic bodies as well as the remaining cell fragments ultimately swell and finally lyse. This terminal phase of in vitro cell death has been termed “secondary necrosis.” Apoptosis 99 can be measured by methods known to those skilled in the art like DNA fragmentation, exposure of Annexin V, activation of caspases, release of cytochrome c, etc. A cell that has been induced to die is termed herein as an “apoptotic cell.” [00277] Apoptosis can also be tested using a standard Annexin V Apoptosis Assay: NIH-.OVCAR-3 cells are grown in 6-well plates (NUNC) and irradiated or treated with an antagonist (or in combination with another anti-cancer drug) for 4-48 hours, washed and stained with Annexin V-FITC (BD-Pharmingen) for 1 hour. Cells are analyzed by flow cytometry (Becton-Dickinson, CellQuest), counterstained with Propidium Iodide and analyzed again in the flow cytometer.

(00278] Patients can be assessed with respect to symptoms at one or more multiple time points including prior to, during, and after treatment regimens. Treatment can result in improving the subject’s condition and can be assessed by determining if one or more of the following factors has occurred: decreased tumor size, decreased cell proliferation, decreased numbers of cells, decreased neovascularization, increased apoptosis, or decreased survival of at least a portion of the tumor cells. One or more of these occurrences may, in some cases, result in partial or total elimination of the cancer and prolongation of survival of the patient. Alternatively, for terminal stage cancers, treatment may result in stasis of disease, better quality of life and/or prolongation of survival.

Methods of Assaying Cell Migration [00279] Assays for cell migration have been described in the literature, e.g., by Brooks, et al., J. Clin. Invest 1997, 99:1390-1398 and methods for measuring cell migration are known to those of skill in the art. In one method for measuring cell migration described herein, membranes from transwell migration chambers are coated with substrate, the transwells washed, and non-specific binding sites blocked with BSA. Tumor cells from sub-confluent cultures are harvested, washed, and resuspended in migration buffer in the presence or absence of assay antibodies. After the tumor cells are allowed to migrate to the underside of the coated transwell membranes, the cells remaining on the top-side of the membrane are removed and cells that migrate to the under-side are stained with crystal violet. Cell migration is then quantified by direct cell counts per microscopic field.

Methods of Assaying Tumor Growth [00280] Tumor growth can be assayed by methods known to those of skill in the art, e.g., the SCID mouse model, the nude mouse model, and BALB/c mice with syngeneic tumors. SCID mouse models for tumor growth are carried out as follows: subconfluent human M21 melanoma cells (or any desired tumor cell type) are harvested, washed, and resuspended in sterile PBS (20 x 106 per mL). SCID mice are injected subcutaneously with 100 pL of M21 human melanoma cell (2 x 106) suspension. Three days after tumor cell injection, mice are either untreated or treated intraperitoneally with an antagonist in the desired dose ranges. The mice are treated daily for 24 days. Tumor size is measured with calipers and the volume estimated using the formula V = (L x W2)/2, where V is equal to the volume, L is equal to the length, and W is equal to the width.

100 [00281] Alternatively, nude mouse models, SCID mouse models and/or BALB/c syngeneic mouse models can also be utilized to assess tumor growth and inhibition thereof by the humanized anti-endoglin antibodies or antigen-binding fragments described herein.

Methods of Assaying Cell Proliferation [00282] Cell proliferation can be assayed by methods known to those of skill in the art. As described herein, subconfluent human endothelial cells (HUVECs) can be resuspended in proliferation buffer containing low (5.0%) serum in the presence or absence of CM (25 pL) from ECV or ECVL cells, and endothelial cells allowed to proliferate for 24 hours. Proliferation can be quantified by measuring mitochondrial dehydrogenase activity using a commercially available WST-1 assay kit (Chemicon). Also, as described herein, proliferation can be quantified by measuring 3H incorporation using standard methods. (She et al., Int. J. Cancer, 108:251-257 (2004)).

[00283] Other methods of assessing cell proliferation are known in the art and are contemplated herein. Further non-limiting examples are described in more detail in the examples.

[00284] One would understand that classification and staging systems described herein represent one means to assess treatment of cancers described herein; additionally, other staging schemes are known in the art and may be used in connection with the methods described herein. By way of example only, the TNM classification of malignant tumors may be used as a cancer staging system to describe the extent of cancer in a patient’s body. T describes the size of the tumor and whether it has invaded nearby tissue, N describes regional lymph nodes that are involved, and M describes distant metastasis. TNM is maintained by the International Union Against Cancer (UICC) and is used by the American Joint Committee on Cancer (AJCC) and the International Federation of Gynecology and Obstetrics (FIGO). One would understand that not all tumors have TNM classifications such as, for example, brain tumors. Generally, T (a,is,(0), 1-4) is measured as the size or direct extent of the primary tumor. N (0-3) refers to the degree of spread to regional lymph nodes: NO means that tumor cells are absent from regional lymph nodes, NI means that tumor cells spread to the closest or small numbers of regional lymph nodes, N2 means that tumor cells spread to an extent between NI and N3; N3 means that tumor cells spread to most distant or numerous regional lymph nodes. M (0/1) refers to the presence of metastasis: M0 means that no distant metastasis are present; Ml means that metastasis has occurred to distant organs (beyond regional lymph nodes). Other parameters may also be assessed. G (1-4) refers to the grade of cancer cells (i.e., they are low grade if they appear similar to normal cells, and high grade if they appear poorly differentiated). R (0/1/2) refers to the completeness of an operation (i.e., resection-boundaries free of cancer cells or not). L (0/1) refers to invasion into lymphatic vessels. V (0/1) refers to invasion into vein. C (1-4) refers to a modifier of the certainty (quality) of V.

[00285] Provided herein are methods for degrading, inhibiting the growth of or killing cancer cells comprising contacting the cells with an amount of a compound described herein effective to degrade, inhibit the growth of or kill cancer cells.

101 [00286] Provided herein are methods of inhibiting tumor size increase, reducing the size of a tumor, reducing tumor proliferation or preventing tumor proliferation in an individual comprising administering to said individual an effective amount of a compound described herein to inhibit tumor size increase, reduce the size of a tumor, reduce tumor proliferation or prevent tumor proliferation. Treatment of tumors in some cases includes stasis of symptoms, that is, by treating the patient, the cancer does not worsen and survival of the patient is prolonged.

[00287] Patients may be assessed with respect to symptoms at one or more multiple time points including prior to, during, and after treatment regimens. Treatment can result in improving the subject’s condition and can be assessed by determining if one or more of the following events has occurred: decreased tumor size, decreased tumor cell proliferation, decreased numbers of cells, decreased neovascularization and/or increased apoptosis. One or more of these occurrences may, in some cases, result in partial or total elimination of the cancer and prolongation of survival of the patient. Alternatively, for terminal stage cancers, treatment may result in stasis of disease, better quality of life and/or prolongation of survival. Other methods of assessing treatment are known in the art and contemplated herein.

[00288] In an exemplary embodiment, the pro-oligonucleotide compounds of the invention are administered to a subject such as a mammal (e.g., a human), suffering from a medical disorder, e.g., a cancer, or nonmalignant conditions characterized by the presence of a class of unwanted cells.

[00289] Primary outcome measures may be assessed for patients treated using the methods described herein and include, for example, progression-free survival. In one embodiment, an increase in progression free survival is observed in an amount of by about 2-fold, 5-fold, 10-fold, 20 fold, 50 fold or more compared to lack of treatment. In another embodiment, an increase in progression free survival is increased survival by about 3 months, about 6 months, about 9 months, about 12 months, about 18 months, about 2 years, about 3 years, about 4 years, about 5 years or more compared to lack of treatment.

[00290] Secondary outcome measures may also be assessed and include duration of response, time to tumor progression, overall survival, serious and non-serious adverse events. For example, a treatment may prevent progression of the disease (i.e., stasis) or may result in an improvement. Alternately, or in addition, other goals can be measured with respect to one or more of the following: decreased tumor burden, decreased neovascularization, reduced side effects, decreased adverse reactions, and/or increased patient compliance.

[00291] Other specific examples of diseases or disorders for which treatment by the compounds or compositions of the invention are useful for treatment or prevention include, but are not limited to transplant rejection (e.g., kidney, liver, heart, lung, islet cells, pancreas, bone marrow, cornea, small bowel, skin allografts or xenografts and other transplants), graft vs. host disease, osteoarthritis, rheumatoid arthritis, multiple sclerosis, diabetes, diabetic retinopathy, inflammatory bowel disease (e.g., Crohn's disease, ulcerative colitis, and other bowel diseases), renal disease, cachexia, septic shock, lupus, myasthenia gravis, psoriasis, dermatitis, eczema, seborrhea, Alzheimer's disease, Parkinson's disease, stem cell protection during chemotherapy, ex vivo selection or ex vivo purging for autologous or allogeneic bone

102 marrow transplantation, ocular disease, retinopathies (e.g., macular degeneration, diabetic retinopathy, and other retinopathies), comeal disease, glaucoma, infections (e.g., bacterial, viral, or fungal), heart disease, including, but not limited to, restenosis.

Activation of RNAse L [00292]The 2-5' oligoadenylate (2-5A)/RNase L pathway is one of the enzymatic pathways induced by interferon. Rnase L is activated after binding to 5’-phosphoroylated fragments of 2’-5’ adenylic acid. These fragments of 2’-5’ adenylic acid (2-5A) are produced under the control of 2’-5’ oligo(A) synthetase. This pathway is part of the innate immune system and has an important role in preventing viral infection. 2-5AInduced cleavage of single-stranded RNA results in apoptosis. Biostable phosphorothioate analogs of 2-5A have been shown to be potent activators of Rnase L (Xianh et al., Cancer Research (2003), 63:6795-6801). In this study, the 2-5A analogs induced Rnase L activity and caused apoptosis in cultures of late-stage, metastatic human prostate cancer cell lines DU145, PC3 and LNCaP.

[00293] Sustained activation of RNase L triggers a mitochondrial pathway of apoptosis that eliminates virusinfected cells as well as cancerous/tumor cells. RNase L can inhibit fibrosarcoma growth, prostate cancer growth, colorectal cancer growth and pancreatic cancer growth. Given the common role of RNase L in different cancers, it is contemplated that the invention described herein can be use for the treatment of any type of cancer. Silverman, RH, Cytokine Growth Factor Rev, 18(5-6): 381-388 (2007); Bisbal, C. and Silverman, RH, Biochimie. 89(6-7): 789-798 (2007). By way of example, downregulation of RNase L refers to any reduction in expression levels of the gene or genes encoding RNase L, silencing of the gene or genes encoding RNase L, reduction in the levels of expression/translation of the proteins comprising RNase L, redudction in the amount of RNase L present within a cell, and/or any reduction in activity of RNase L as compared to a predetermined level of RNase L in an exemplary healthy population. Alternatively any reduction in RNase L levels as described herein can be indicative of downregulation of RNase L.

In one exemplary embodiment, the compounds described herein are useful for the treatment of diseases having downregulated RNase L. In another embodiment, the disease associated with downregulated RNase L is cancer. In further embodiments, the cancer is pancreatic cancer, prostate cancer, or colorectal cancer. Alternatively, the compounds described herein are useful for the treatment of disease having upregulated RNase L. In one exemplary embodiment, the disease having upregulated RNase L is chronic fatigue syndrome. Additional diseases having upregulated RNase L are known in the art and contemplated herein. [00294] When used as therapeutics, the nucleic acid described herein is administered as a pharmaceutical composition. In some embodiments, the pharmaceutical composition comprises a therapeutically effective amount of a nucleic acid comprising a chiral X-phosphonate moiety of Formula 1, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable inactive ingredient selected from pharmaceutically acceptable diluents, pharmaceutically acceptable excipients, and pharmaceutically acceptable carriers. In another embodiment, the pharmaceutical composition is formulated for intravenous injection, oral administration, buccal administration, inhalation, nasal administration, topical administration, 103 ophthalmic administration or otic administration. In further embodiments, the pharmaceutical composition is a tablet, a pill, a capsule, a liquid, an inhalant, a nasal spray solution, a suppository, a suspension, a gel, a colloid, a dispersion, a suspension, a solution, an emulsion, an ointment, a lotion, an eye drop or an ear drop. Pharmaceutical Compositions and Administration [00295] In another aspect, the present invention provides a pharmaceutical composition comprising a nonracemic pro-oligonucleotide in admixture with a pharmaceutically acceptable excipient. One of skill in the art will recognize that the pharmaceutical compositions include the pharmaceutically acceptable salts of the non-racemic pro-oligonucleotides described above.

Compounds for enhancing and targeting delivery [00296] The pro-oligonucleotides described herein can be delivered using a variety of delivery strategies, including conjugates of oligonucleotides with various ligands, as well as use of nanocarrier approaches. Any nucleic acid delivery strategies are contemplated for use with the pro-oligonucleotides described herein. The choice between exemplary delivery strategies, including but not limited to, chemical conjugates, cationic lipid/liposomal transfer vessicles and supramolecular nanocarriers depends on the therapeutic context, and methods for determining the optimal delivery modality are known in the art and further contemplated herein. Cell Penetrating Compounds (CPCs) [00297] Numerous compounds are known to act as carriers for cargo such as nucleic acids and facilitate entry of the nucleic acid in a cell in an in vivo setting. Exemplary carriers are described in Dietz et al., Molecular &amp; Cellular Neuroscience, 27(2): 85-131 (2004) which is incorporated herein by reference. The prototypical CPCs derived from the Tat and antennepedia transcriptional regulators have been joined by a large number of new moieties. As an example, CPCs that are peptides can be relatively short (9-30 amino acids) polycationic peptides rich in argine and lysine, or membrane-interactive hydrophobic sequences. CPCs can be linked by recombinant DNA techniques or chemically coupled to peptides, oligonucleotides or nanocarriers, which then comprise the ‘cargo’ for the CPC.

Cell Targeting Ligands (CTLs) [00298] Another strategy is to deliver oligonucleotides by use of a CTL that binds with high affinity to a cell surface receptor that is capable of undergoing efficient internalization. Potential ligands include antibodies, polypeptides derived from phage display libraries, and small organic molecules. Additional cell-targeting ligands are known in the art, or will be developed, and are contemplated for use with the invention described herein. Because various receptors are often preferentially expressed on particular cell types, this approach offers the possibility of improved selectivity for the oligonucleotide reagents. Exemplary receptor targets include, but are not limited to, lipoprotein receptors (such as those in the liver), integrins, receptor tyrosine kinases, and the G-protein coupled receptor (GPCR) superfamily.

Nanocarriers [00299] A variety of supramolecular nanocarriers can be used to deliver nucleic acids. Exemplary nanocarriers include, but are not limited to liposomes, cationic polymer complexes and various polymeric.

104

Complexation of nucleic acids with various polycations is another approach for intracellular delivery; this includes use of PEGlyated polycations, polyethyleneamine (PEI) complexes, cationic block co-polymers, and dendrimers. Several cationic nanocarriers, including PEI and polyamidoamine dendrimers help to release contents from endosomes. Other approaches include use of polymeric nanoparticles, polymer micelles, quantum dots and lipoplexes.

[00300] Additional nucleic acid delivery strategies are known in addition to the exemplary delivery strategies described herein.

[00301] In therapeutic and/or diagnostic applications, the compounds of the invention can be formulated for a variety of modes of administration, including systemic and topical or localized administration. Techniques and formulations generally may be found in Remington, The Science and Practice of Pharmacy, (20th ed. 2000).

[00302] The compounds according to the invention are effective over a wide dosage range. For example, in the treatment of adult humans, dosages from 0.01 to 1000 mg, from 0.5 to 100 mg, from 1 to 50 mg per day, and from 5 to 100 mg per day are examples of dosages that may be used. The exact dosage will depend upon the route of administration, the form in which the compound is administered, the subject to be treated, the body weight of the subject to be treated, and the preference and experience of the attending physician.

[00303] Pharmaceutically acceptable salts are generally well known to those of ordinary skill in the art, and may include, by way of example but not limitation, acetate, benzenesulfonate, besylate, benzoate, bicarbonate, bitartrate, bromide, calcium edetate, camsylate, carbonate, citrate, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isethionate, lactate, lactobionate, malate, maleate, mandelate, mesylate, mucate, napsylate, nitrate, pamoate (embonate), pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, sulfate, tannate, tartrate, or teoclate. Other pharmaceutically acceptable salts may be found in, for example, Remington, The Science and Practice of Pharmacy (20th ed. 2000). Preferred pharmaceutically acceptable salts include, for example, acetate, benzoate, bromide, carbonate, citrate, gluconate, hydrobromide, hydrochloride, maleate, mesylate, napsylate, pamoate (embonate), phosphate, salicylate, succinate, sulfate, or tartrate.

[00304] Depending on the specific conditions being treated, such agents may be formulated into liquid or solid dosage forms and administered systemically or locally. The agents may be delivered, for example, in a timed- or sustained- low release form as is known to those skilled in the art. Techniques for formulation and administration may be found in Remington, The Science and Practice of Pharmacy (20th ed. 2000). Suitable routes may include oral, buccal, by inhalation spray, sublingual, rectal, transdermal, vaginal, transmucosal, nasal or intestinal administration; parenteral delivery, including intramuscular, subcutaneous, intramedullary injections, as well as intrathecal, direct intraventricular, intravenous, intra-articullar, intrastemal, intra-synovial, intra-hepatic, intralesional, intracranial, intraperitoneal, intranasal, or intraocular injections or other modes of delivery.

105 [00305] For injection, the agents of the invention may be formulated and diluted in aqueous solutions, such as in physiologically compatible buffers such as Hank's solution, Ringer's solution, or physiological saline buffer. For such transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art.

[00306] Use of pharmaceutically acceptable inert carriers to formulate the compounds herein disclosed for the practice of the invention into dosages suitable for systemic administration is within the scope of the invention. With proper choice of carrier and suitable manufacturing practice, the compositions of the present invention, in particular, those formulated as solutions, may be administered parenterally, such as by intravenous injection. The compounds can be formulated readily using pharmaceutically acceptable carriers well known in the art into dosages suitable for oral administration. Such carriers enable the compounds of the invention to be formulated as tablets, pills, capsules, liquids, gels, syrups, slurries, suspensions and the like, for oral ingestion by a subject (e.g., patient) to be treated.

[00307] For nasal or inhalation delivery, the agents of the invention may also be formulated by methods known to those of skill in the art, and may include, for example, but not limited to, examples of solubilizing, diluting, or dispersing substances such as, saline, preservatives, such as benzyl alcohol, absorption promoters, and fluorocarbons.

[00308] Pharmaceutical compositions suitable for use in the present invention include compositions wherein the active ingredients are contained in an effective amount to achieve its intended purpose. Determination of the effective amounts is well within the capability of those skilled in the art, especially in light of the detailed disclosure provided herein.

[00309] In addition to the active ingredients, these pharmaceutical compositions may contain suitable pharmaceutically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. The preparations formulated for oral administration may be in the form of tablets, dragees, capsules, or solutions.

[00310] Pharmaceutical preparations for oral use can be obtained by combining the active compounds with solid excipients, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carboxymethyl-cellulose (CMC), and/or polyvinylpyrrolidone (PVP: povidone). If desired, disintegrating agents may be added, such as the cross-linked polyvinylpyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate.

[00311] Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol (PEG), and/or titanium dioxide, lacquer solutions, and suitable organic solvents or

106 solvent mixtures. Dye-stuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.

[00312] Pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin, and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols (PEGs). In addition, stabilizers may be added.

[00313] Depending upon the particular condition, or disease state, to be treated or prevented, additional therapeutic agents, which are normally administered to treat or prevent that condition, may be administered together with the inhibitors of this invention. For example, chemotherapeutic agents or other antiproliferative agents may be combined with the inhibitors of this invention to treat proliferative diseases and cancer. Examples of known chemotherapeutic agents include, but are not limited to, adriamycin, dexamethasone, vincristine, cyclophosphamide, fluorouracil, topotecan, taxol, interferons, and platinum derivatives.

[00314] Other examples of agents the non-racemic pro-oligonucleotide of this invention may also be combined with include, without limitation, anti-inflammatory agents such as corticosteroids, TNF blockers, IL-1 RA, azathioprine, cyclophosphamide, and sulfasalazine; immunomodulatory and immunosuppressive agents such as cyclosporin, tacrolimus, rapamycin, mycophenolate mofetil, interferons, corticosteroids, cyclophophamide, azathioprine, and sulfasalazine; neurotrophic factors such as acetylcholinesterase inhibitors, MAO inhibitors, interferons, anti-convulsants, ion channel blockers, riluzole, and antiParkinsonian agents; agents for treating cardiovascular disease such as beta-blockers, ACE inhibitors, diuretics, nitrates, calcium channel blockers, and statins; agents for treating liver disease such as corticosteroids, cholestyramine, interferons, and anti-viral agents; agents for treating blood disorders such as corticosteroids, anti-leukemic agents, and growth factors; agents for treating diabetes such as insulin, insulin analogues, alpha glucosidase inhibitors, biguanides, and insulin sensitizers; and agents for treating immunodeficiency disorders such as gamma globulin.

[00315] These additional agents may be administered separately, as part of a multiple dosage regimen, from the non-racemic pro-oligonucleotide-containing composition. Alternatively, these agents may be part of a single dosage form, mixed together with the non-racemic pro-oligonucleotide in a single composition.

[00316] The examples and preparations provided below further illustrate and exemplify the compounds of the present invention and methods of preparing such compounds. It is to be understood that the scope of the present invention is not limited in any way by the scope of the following examples and preparations.

107

EXAMPLES

Example 1: The synthesis of (SP)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(terfbutyldiphenylsilyl)thymidin-3'-yl 3'-O-(iert-butyldimethylsilyl)thymidin-5'-yl phosphorothioate [(5P)-4tt] is illustrated in Scheme A.

Scheme A

TBDPSl

TBDI

ConcNhb

55’C, 12 h

TBDI

OTBS

4tt [00317] 8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(iert-butyldiphenylsilyl)thymidin-3'-yl phosphonate (It) (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). ?7//’-Bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl; 500 pmol) is added and the mixture is stirred for 5 min. A solution of amino alcohol (L-2) (100 pmol) is repeatedly coevaporated with dry pyridine and dissolved in dry pyridine (1 mL). The aminoalcohol solution is added to the reaction mixture dropwise via syringe, and the mixture is stirred for 5 min under argon. 3'-O-(terr-butyldimethylsilyl)thymidine 3t is dried using repeated coevaporations with dry pyridine and dissolved in 100 pmol pyridine. The above mixture is added via cannula to the solution of 3'-O-(teri-butyldimethylsilyl)thymidine 3t in dry (100 pmol) pyridine. After 5 min, N-trifluoroacetyl imidazole (CF3COIm; 200 pmol) is added. After an additional 30 s, Ν,Ν’dimethylthiuram disulfide (DTD; 120 pmol) is added. Following an additional 3 min, the mixture is dried in vacuum. Concentrated NH3 (10 mL) is added to the residue, and the mixture is heated for 12 h at 55 °C. The mixture is then allowed to cool to room temperature and then concentrated to dryness under reduced pressure. The mixture is diluted with CHC13 (5 mL), and washed with 0.2 M phosphate buffer (pH 7.0, 5 mL). The aqueous layers are back-extracted with CHC13 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness under reduced pressure. The residue is purified by PTLC.

108

The product is dissolved in CHC13 (5 mL), washed with 0.2 M l,8-diazabicyclo[5.4.0]undec-7-enium bicarbonate buffer (5 mL) and back-extracted with CHC13 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness to afford (SP)-4tt.

Example 2. The synthesis of (S_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 6-AM&gt;enzoyl-5'-O-(terrbutyldiphenylsilyl)-deoxyadenosin-3 '-yl 3 '-O-(ieri-butyldimethylsilyl)thymidin-5 '-y 1 phosphorothioate [(S_P)4at].

[00318] (SP)-4at is obtained from l,8-diazabicyclo[5.4.0]undec-7-enium 6-N-benzoyl-5'-O-(teributyldiphenylsilyl)- deoxyadenosin-3'-yl phosphonate (la) instead of It, using the reaction steps described in Example 1 and Scheme A for (SP)-4tt.

Example 3. The synthesis of (S_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 4-yV-benzoyl-5'-O-(ieributyldiphenylsilyl)-deoxycytidin-3 '-yl 3 '-O-(tert-butyldimethylsilyl)thymidin-5 '-yl phosphorothioate [(S_P)4ct].

[00319] (SP)-4ct is obtained from l,8-diazabicyclo[5.4.0]undec-7-enium 4-7V-benzoyl-5'-O-(iertbutyldiphenylsilyl)-deoxycytidin-3'-yl phosphonate (lc) ,instead of It, using the reaction steps described in Example 1 and Scheme A for (SP)-4tt.

Example 4. The synthesis of (S_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 2-2V-phenoxyacetyl-5'-O-(terrbutyldiphenylsilyl)deoxyguanosin-3 '-yl 3 '-O-(ieri-butyldimethylsilyl)thymidin-5 '-yl phosphorothioate [(S_P)4gt].

[00320](SP)-4gt is obtained from l,8-diazabicyclo[5.4.0]undec-7-enium 2-2V-phenoxyacetyl-5'-O-(tertbutyldiphenylsilyl)deoxyguanosin-3'-yl phosphonate (lg) instead of It, using the reaction steps described in Example 1 and Scheme A for (SP)-4tt.

Example 5. The synthesis of (/?_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(tertbutyldiphenylsilyl)thymidin-3'-yl 3'-O-(teri-butyldimethylsilyl)thymidin-5'-yl phosphorothioate [(7?p)-4tt], ]00321](7?P)-4tt is produced via the transformations described in Example 1 and Scheme A for the synthesis of (5P)-4tt using the amino alcohol D-2 as a chiral reagent, instead of L-2.

HQ

Ph'

D-2

Example 6. The synthesis of (Z?_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 6-TV-benzoy 1-5'-O-(tertbutyldiphenylsilyl)deoxyadenosin-3'-yl 3'-O-(tert-butyldimethylsilyl)thymidin-5'-yl phosphorothioate [(R_P)4at], [00322](7?P)-4at is produced via the transformations described in Example 2 using compound la and the amino alcohol D-2 as a chiral reagent, instead of L-2.

109

Example 7. The synthesis of (5_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 4-77-benzoyl-5'-O-(ieributyldiphenylsilyl)deoxycytidin-3 '-yl 3 '-O-(7eri-butyldimethylsilyl)thymidin-5 '-yl phosphorothioate [(R_P)4ct].

[00323] (RP)-4ct is produced via the transformations described above in Example 3 using compound lc and the amino alcohol D-2 as a chiral reagent, instead of L-2.

Example 8. The synthesis of (7?_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 2-N-phenoxyacetyl-5'-O-(tertbutyldiphenylsilyl)deoxyguanosin-3 '-yl 3 '-O-(ierNbutyldimethylsilyl)thymidin-5 '-yl phosphorothioate [(Λ_Ρ)4gt].

[00324] (RP)-4gt is produced via the transformations described above in Example 4 using compound lg and the amino alcohol D-2 as a chiral reagent, instead of L-2.

Scheme B

TBD

<img file="IN720DEN2012A_D0352.tif" />

h^xFohdbu

1t

<img file="IN720DEN2012A_D0353.tif" />

Bopa pyridine

<img file="IN720DEN2012A_D0354.tif" />

(a^2SH pyridne

<img file="IN720DEN2012A_D0355.tif" />

Example 9. Synthesis of (/?_P)-5'-O-(terr-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl //-phosphonate [(R_P)-7tt] as described in Scheme B.

[00325] It (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). AUV’-Bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl; 500 pmol) is added, and the mixture is stirred for 5 min. To the mixture, a solution of amino alcohol ((αΛ, 25)-6) (100 pmol), which has been dried by coevaportions with dry pyridine and dissolved in dry pyridine (1 mL), is added dropwise via syringe, and the mixture is stirred for 5 min under argon. 3'-O-(terNbutyldimethylsilyl)thymidine is dried using repeated coevaporations with dry pyridine and dissolved in 100 pmol pyridine. The above mixture is added via cannula to the solution of 3'-O-(teri-butyldimethylsilyl)thymidine 3t in dry (100 pmol) pyridine. After 15 min, the mixture is concentrated under reduced pressure. The residue is diluted with CH2C12 (5 mL), and washed with saturated NaHCO3 (3x5 mL). The combined aqueous layers are back-extracted with with CH2C12 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to ca. 1 mL under reduced pressure. The residue is added dropwise via a syringe to a stirred 1 % trifluoroacetic acid (TFA) solution in dry CH2C12 (20 mL) at 0 °C. After an additional 5 min, the mixture is diluted with dry CH2C12 (100 mL), and washed with saturated NaHCO3 aqueous solutions (2 χ 100 mL). The combined 110 aqueous layers are back-extracted with CH2C12 (2 χ 100 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness under reduced pressure to afford crude (7?P)-7tt.

Example 10. Synthesis of (5_P)-6-jV-benzoyl-5'-0-(ierZ-butyldiphenylsilyl)deoxyadenosin-3'-yl 3'-O-(Zer/butyldimethylsilyl)thymidin-5'-yl 77-phosphonate [(J?_P)-7at].

[00326] Crude (AP)-7at is produced as described in Example 9 using la instead of It.

Example 11. Synthesis of (3?_P)-4-JV-benzoyl-5'-O-(reri-butyldiphenylsilyl)deoxycytidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl 77-phosphonate [(J?_P)-7ct].

[00327]Crude (7?P)-7ct is produced as described in Example 9 using lc instead of It.

Example 12. Synthesis of (J?_P)-2-/V-phenoxyacetyl-5'-(2-(terr-butyldiphenylsilyl)deoxyguanosin-3'-yl 3-(7(ieri-butyldimethylsilyl)thymidin-5'-yl 77-phosphonate [(2?_P)-7gt].

[00328]Crude (7?P)-7gt is produced as described in Example 9 using lg instead of It.

Example 13. Synthesis of (5_P)-5'-(7-(terr-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl 77-phosphonate [(5_P)-7tt].

[00329] Crude (5P)-7tt is produced as described in Example 9 using (aS, 27()-6 instead of (aR, 2S)-6 as a chiral reagent.

Example 14. Synthesis of (5_P)-6-/V-benzoyl-5'-(7-(terr-butyldiphenylsilyl)deoxyadenosin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl /7-phosphonate [(5_P)-7at[.

[00330]Crude (5P)-7at is produced as described in Example 9 using compound la and (α5, 27()-6 instead of (aR, 25)-6 as a chiral reagent.

Example 15. Synthesis of (5_P)-4-7V-benzoyl-5'-(7-(ieri-butyldiphenylsilyl)deoxycytidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl 77-phosphonate [(5_P)-7ct].

[00331]Crude (5P)-7ct is produced as described in Example 9 using compound lc and (aS, 2R/-6 instead of (aR, 2S)-6 as a chiral reagent.

Example 16. Synthesis of(5_P)-2-/V-phenoxyacetyl-5'-(7-(ter7-butyldiphenylsilyl)deoxyguanosin-3'-yl 3'-O(teri-butyldimethylsilyl)thymidin-5'-yl /7-phosphonate [(5_P)-7gt], [00332]Crude (5P)-7gt is produced as described in Example 9 using compound lg instead of It and compound (aS, 2R)-6 instead of compound (aR, 25)-6 as a chiral reagent.

Scheme C

111

<img file="IN720DEN2012A_D0356.tif" />

<img file="IN720DEN2012A_D0357.tif" />

TBD concNhb-EtOH (3:1, v/v) rt, 12 h

<img file="IN720DEN2012A_D0358.tif" />

IBSO OTBS

10uu

Example 17. Synthesis of (S_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(tert-butyldiphenylsilyl)-2'-O(tert-butyldimethylsilyl)uridin-3 '-y 12',3 '-O-bis(feri-butyldimethylsilyl)uridin-5 '-y 1 phosphorothioate [(S_P)10uu], [00333] 1,8-Diazabicyclo[5.4.0]undec-7-enium 5'-&lt;7-(teri-butyldiphenylsilyl)-2'-O-(ieributyldimethylsilyl)uridin-3'-yl phosphonate (8u) (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). A'A^r’-Bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl; 500 pmol) is added, and the mixture is stirred for 5 min. To the mixture, a solution of amino alcohol (L-2) (100 pmol), which has been dried by repeated coevaportions with dry pyridine and dissolved in dry pyridine (1 mL), is added dropwise via syringe, and the mixture is stirred for 5 min under argon. 2',3'-Obis(tert-butyldimethylsilyl)uridine 9u is dried by repeated coevaporations with dry pyridine and dissolved in 100 pmol pyridine. Then the above mixture is added via cannula into the solution of 2',3'-O-bis(teributyldimethylsilyl)uridine 9u (100 pmol). After 10 min, jV-trifluoroacetyl imidazole (CF3COIm; 200 pmol) is added. After an additional 30 s, Nfl’-dimethylthiuram disulfide (DTD; 120 pmol) is added. After an additional 3 min, the mixture is dried in vacuum. To the residue, cone NH3-EtOH (3:1, v/v, 10 mL) is added, and the mixture is stirred for 12 h, and then concentrated to dryness under reduced pressure. Then, the mixture is diluted with CHC13 (5 mL), and washed with 0.2 M phosphate buffer (pH 7.0, 5 mL). The aqueous layers are back-extracted with CHC13 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness under reduced pressure. The residue is purified by PTLC. The product is dissolved in CHC13 (5 mL), washed with 0.2 M l,8-diazabicyclo[5.4.0]undec-7-enium

112 bicarbonate buffer (5 mL) and back-extracted with CHC13 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness to afford (SB)-lOuu.

Example 18. Synthesis of (S_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 6-N-bemoy\-5'-O-(tertbutyldiphenylsilyl)-2'-O-(tert-butyldimethylsilyl)adenosin-3'-yl 2',3'-&lt;9-bis(iert-butyldimethylsilyl)uridin-5'yl phosphorothioate [(S_P)-10auJ.

]00334](SB)-10au is produced as described in Example 17 using l,8-diazabicyclo[5.4.0]undec-7-enium 6-Nbenzoyl-5'-O-(teri-butyldiphenylsilyl)-2'-O-(terr-butyldimethylsilyl)adenosin-3'-yl phosphonate (8a) instead of 8u.

Example 19. Synthesis of (S_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 4-?/-benzoyl-5'-O-(ierrbutyldiphenylsilyl)- 2'-&lt;9-(ieri-butyldimethylsilyl)cytidin-3'-yl 2',3'-O-bis(ieri-butyldimethylsilyl)uridin-5'-yl phosphorothioate [(S_P)-10cu].

[00335](SB)-lOcu is produced as described in Example 17 using l,8-diazabicyclo[5.4.0]undec-7-enium 4-Nbenzoyl-5'-O-(tert-butyldiphenylsilyl)-2'-O-(ieri-butyldimethylsilyl)cytidin-3'-yl phosphonate (8c) instead of 8u.

Example 20. Synthesis of (S_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 2-N-phenoxyacetyl-5'-O-(teributyldiphenylsilyl)- 2'-O-(teri-butyldimethylsilyl)guanosin-3 '-yl 2',3 '-O-bis(teri-butyldimethylsilyl)uridin-5 'yl phosphorothioate [(S_P)-10gu].

]00336](SB)-10gu is produced as described in Example 17 using l,8-diazabicyclo[5.4.0]undec-7-enium 2-Nphenoxyacetyl-5 '-0-(tert-butyldiphenylsilyl)-2'-0-(teri-butyldimethylsilyl)guanosin-3 '-yl phosphonate (8g) instead of 8u.

Example 21. Synthesis of (R_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(tert-butyldiphenylsilyl)-2'-O(teri-butyldimethylsilyl)uridin-3 '-yl 2 ',3 '-O-bis(iert-butyldimethylsilyl)uridin-5 '-y 1 phosphorothioate [(R_P)10uu], [00337] (Rp)-lOuu is produced as described in Example 17 using chiral reagent D-2 instead of chiral reagent L-2.

Example 22. Synthesis of (R_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 6-JV-benzoyl-5'-0-(teributyldiphenylsilyl)-2'-O-(iert-butyldimethylsilyl)adenosin-3'-yl 2',3'-O-bis(fert-butyldimethylsilyl)uridin-5'yl phosphorothioate [(R_P)-10au[.

[00338] (RP)-lOau is produced as described in Example 17 using 8a instead of 8u and chiral reagent D-2 instead of chiral reagent L-2.

Example 23. Synthesis of (R_P)-1,8-Diazabicyclo[5.4.0]undec-7-enium 4-N-benzoyl-5'-O-(iertbutyldipheny Is ily 1)-2 '-O-(ieri-butyldimethylsilyl)cytidin-3 '-yl 2 ',3 '-O-bis(iert-butyldimethylsilyl)uridin-5 '-yl phosphorothioate [(R_P)-10cu], [00339] (RP)-lOcu is produced as described in Example 17 using 8c instead of 8u and chiral reagent D-2 instead of chiral reagent L-2.

113

Example 24. Synthesis of (R_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 2-N-phenoxyacetyl-5'-O-(tertbutyldiphenylsilyl)-2'-O-(iert-butyldimethylsilyl)guanosin-3'-yl 2',3'-O-bis(tert-butyldimethylsilyl)uridin-5'yl phosphorothioate [(7?_P)-10gu].

[00340] (RP)-lOgu is produced as described in Example 17 using 8g instead of 8u and chiral reagent D-2 instead of chiral reagent L-2.

Scheme D

TBDPSf

TBDI

TBSO OTBS &lt;u

Q OTBS

TBDPSI

TBSO OTBS

Example 25. Synthesis of (7?_P)-5'-O-(iert-butyldiphenylsilyl)-2'-O-(iert-butyldimethylsilyl)uridin-3'-yl 2',3'O-bis(tert-butyldimethylsilyl)uridin-5 '-y 1 H-phosphonate [(2?_P)-12uu].

[00341] 8u (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). AT^V’-Bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl; 500 pmol) is added, and the mixture is stirred for 5 min. To the mixture, a solution of amino alcohol ((αΛ, 2S)-6) (100 pmol), which is dried by coevaportions with dry pyridine and dissolved in dry pyridine (1 mL), is added dropwise via syringe, and the mixture is stirred for 5 min under argon. Then the mixture is added via cannula into a solution of 9u (100 pmol), which is prepared by repeated coevaporations with dry pyridine and dissolution in pyridine. After 15 min, the mixture is concentrated under reduced pressure. The residue is diluted with CH2C12 (5 mL), and washed with saturated NaHCO3 (3x5 mL). The combined aqueous layers are backextracted with with CH2C12 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to ca. 1 mL under reduced pressure. The residue is added dropwise via a syringe to a stirred 1% trifluoroacetic acid (TFA) solution in dry CH2C12 (20 mL) at 0 °C. After an additional 5 min, the mixture is diluted with dry CH2C12 (100 mL), and washed with saturated NaHCO3 aqueous solutions (2 x 100 mL). The combined aqueous layers are back-extracted with CH2C12 (2 x 100 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness under reduced pressure to afford crude (Rp)12uu, which is analyzed by 3IP NMR.

114

Example 26. Synthesis of (Rp)-6-N-Benzoyl-5'-O-(iert-butyldiphenylsilyl)-2'-O-(ieributyldimethylsilyl)adenosin-3 '-yl 2 ',3 '-O-bis(terr-butyldimethylsilyl)uridin-5 '-yl //-phosphonate [(R_P)-12au]. [00342] Crude (RP)-12au is produced as described in Example 25 using 8a instead of 8u.

Example 27. Synthesis of (/?p)-4-AT-Benzoyl-5'-O-(iert-butyldiphenylsilyl)-2'-O-(rerrbutyldimethylsilyl)cytidin-3 '-yl 2',3 '-O-bis(teri-butyldimethylsilyl)uridin-5 '-yl //-phosphonate [(R_f)-12cu]. [00343] Crude (RP)-12cu is produced as described in Example 25 using 8c instead of 8u.

Example 28. Synthesis of (Rp)-2-A/-Phenoxyacetyl-5'-O-(reri-butyldiphenylsilyl)-2'-O-(rerrbutyldimethylsilyl)guanosin-3 '-yl 2',3 '-O-bis(rm-butyldimethylsilyl)uridin-5 '-yl //-phosphonate [(Rp)-12gu]. [00344] Crude (RP)-12gu is produced as described in Example 25 using 8g instead of 8u.

Example 29. Synthesis of (Sp)-5'-O-(teri-butyldiphenylsilyl)-2'-O-(ieri-butyldimethylsilyl)uridin-3'-yl 2',3'O-bis(ierr-butyldimethylsilyl)uridin-5 '-yl //-phosphonate [(S_P)-12uu].

[00345] Crude (SP)-12uu is produced as described in Example 25 using chiral reagent (aS, 2R)-6 instead of chiral reagent (aR, 2S)-6.

Example 30. Synthesis of (S_P)-6W-Benzoyl-5'-O-(ieri-butyldiphenylsilyl)-2'O-(ieributyldimethylsilyl)adenosin-3 '-yl 2',3 '-O-bis(ieri-butyldimethylsilyl)uridin-5 '-yl //-phosphonate [(S_P)-12au]. [00346] Crude (SP)-12au is produced as described in Example 25 using 8a instead of 8u and chiral reagent (aS, 2R)-6 instead of chiral reagent (a/?, 2S)-6.

Example 31. Synthesis of (S_P)-4-A-Benzoyl-5'-0-(ieri-butyldiphenylsilyl)-2'-0-(ieributyldimethylsilyl)cytidin-3 '-yl 2',3 '-O-bis(teri-butyldimethylsilyl)uridin-5 '-y 1 //-phosphonate [(S_P)-12cu]. [00347] Crude (SP)-12cu is produced as described in Example 25 using 8c instead of 8u and chiral reagent (aS, 2R)-6 instead of chiral reagent (aR, 2S)-6.

Example 32. Synthesis of (S_P)-2-?/-Phenoxyacetyl-5'-0-(ierr-butyldiphenylsilyl)-2'-0-(rerrbutyldimethylsilyl)guanosin-3 '-yl 2',3 '-O-bis(iert-butyldimethylsilyl)uridin-5'-yl //-phosphonate [(S_P)-12gu]. [00348] Crude (SP)-12gu is produced as described in Example 25 using 8g instead of 8u and chiral reagent (aS, 2R)-6 instead of chiral reagent (aR, 2S)-6.

Scheme E

TBDPSO-ί λ

V?

TBSO CK^O HDBIT O'^B'H

<img file="IN720DEN2012A_D0359.tif" />

pyridine

<img file="IN720DEN2012A_D0360.tif" />

pyridne

<img file="IN720DEN2012A_D0361.tif" />

pyridne

13u

FscAhQ

CFaCOlm

<img file="IN720DEN2012A_D0362.tif" />

concNHfe-EtOH (3:1, v/v) rt,12h pyridine

<img file="IN720DEN2012A_D0363.tif" />

TBSO

14uu

115

Example 33. Synthesis of (S_P)-1,8-Diazabicyclo[5.4.0]undec-7-enium 5'-(?-(teri-butyldiphenylsilyl)-3'-O(tert-butyldimethylsilyl)uridin-2'-yl 2',3'-&lt;2-bis(ieri-butyldimethylsilyl)uridin-5'-yl phosphorothioate [($&gt;)14uu], [00349] 1,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(iert-butyldiphenylsilyl)-3'-O-(teributyldimethylsilyl)uridin-3'-yl phosphonate (13u) (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). 77^’-Bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl; 500 pmol) is added, and the mixture is stirred for 5 min. To the mixture, a solution of amino alcohol (L-2) (100 pmol), which has been dried by repeated coevaportions with dry pyridine and dissolved in dry pyridine (1 mL), is added dropwise via syringe, and the mixture is stirred for 5 min under argon. 2',3'-Obis(ieri-butyldimethylsilyl)uridine 9u is dried by repeated coevaporations with dry pyridine and dissolved in 100 pmol pyridine. Then the above mixture is added via cannula into the solution of 2',3'-O-bis(tertbutyldimethylsilyl)uridine 9u (100 pmol). After 10 min, TV-trifluoroacetyl imidazole (CF3COIm; 200 pmol) is added. After an additional 30 s, NJl ’-dimethylthiuram disulfide (DTD; 120 pmol) is added. After an additional 3 min, the mixture is dried in vacuum. To the residue, cone NH3-EtOH (3:1, v/v, 10 mL) is added, and the mixture is stirred for 12 h, and then concentrated to dryness under reduced pressure. Then, the mixture is diluted with CHC13 (5 mL), and washed with 0.2 M phosphate buffer (pH 7.0, 5 mL). The aqueous layers are back-extracted with CHC13 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness under reduced pressure. The residue is purified by PTLC. The product is dissolved in CHC13 (5 mL), washed with 0.2 M l,8-diazabicyclo[5.4.0]undec-7-enium bicarbonate buffer (5 mL) and back-extracted with CHC13 (2x5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness to afford (SP)-14uu.

Example 34. Synthesis of (5_P)-1,8-Diazabicyclo[5.4.0]undec-7-enium 6-7V-benzoyl-5'-O-(teributyldiphenylsilyl)-3'-O-(teri-butyldimethylsilyl)adenosin-2'-yl 2',3'-O-bis(ieri-butyldimethylsilyl)uridin-5'yl phosphorothioate [(S_P)-14au[.

[00350] (SP)-Mau is produced as described in Example 33 using l,8-diazabicyclo[5.4.0]undec-7-enium 6-Nbenzoy 1-5 '-&lt;9-(iert-butyldiphenylsilyl)-3 '-O-(ieri-butyldimethylsilyl)adenosin-2 '-yl phosphonate (13 a) instead of 13u.

Example 35. Synthesis of (S_P)-1,8-Diazabicyclo[5.4.0]undec-7-enium 4-A^f-benzoyl-5'-O-(teributyldiphenylsilyl)- 3'-O-(teri-butyldimethylsilyl)cytidin-2'-yl 2',3'-&lt;9-bis(ieri-butyldimethylsilyl)uridin-5'-yl phosphorothioate [(5_P)-14cu].

[00351](SP)-14cu is produced as described in Example 33 using l,8-diazabicyclo[5.4.0]undec-7-enium 4-Nbenzoyl-5'-O-(tert-butyldiphenylsilyl)-3 '-O-(ieri-butyldimethylsilyl)cytidin-2'-yl phosphonate (13c) instead of 13u.

Example 36. Synthesis of (5^)-1,8-Diazabicyclo[5.4.0]undec-7-enium 2-jV-phenoxyacetyl-5'-O-(teributyldiphenylsilyl)- 3 '-O-(teri-butyldimethylsilyl)guanosin-2'-yl 2',3 '-O-bis(teri-butyldimethylsilyl)uridin-5 'yl phosphorothioate [(S_P)-14gu],

116 [00352] (5P)-14gu is produced as described in Example 33 using l,8-diazabicyclo[5.4.0]undec-7-enium 2-Nphenoxyacetyl-5 '-O-(ieri-butyldiphenylsilyl)-3 '-O-(ieri-butyldimethylsily l)guanosin-2'-y 1 phosphonate (13 g) instead of 13u.

Example 37. Synthesis of (A_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(teri-butyldiphenylsilyl)-3'-O(tert-butyldimethylsilyl)uridin-2'-yl 2',3'-O-bis(teri-butyldimethylsilyl)uridin-5'-yl phosphorothioate [(5p)14uu], [00353] (5p)-14uu is produced as described in Example 33 using chiral reagent D-2 instead of chiral reagent L-2.

Example 38. Synthesis of (A_P)-1 ,8-Diazabicyclo[5.4.0]undec-7-enium 6-7V-benzoyl-5'-O-(teributyldiphenylsilyl)-3'-O-(tert-butyldimethylsilyl)adenosin-2'-yl 2',3'-O-bis(teri-butyldimethylsilyl)uridin-5'yl phosphorothioate [(5_P)-14auJ.

[00354] (AP)-Mau is produced as described in Example 33 using 13a instead of 13u and chiral reagent D-2 instead of chiral reagent L-2.

Example 39. Synthesis of (A_P)-1,8-Diazabicyclo[5.4.0]undec-7-enium 4-N-benzoyl-5'-O-(teributyldiphenylsilyl)-3 '-O-(tert-buty ldimethylsilyl)cytidin-2 '-y 12 ',3 '-O-bis(teri-butyldimethylsilyl)uridin-5 '-y 1 phosphorothioate [(A_P)-14cu], [00355] (AP)-14cu is produced as described in Example 33 using 13c instead of 13u and chiral reagent D-2 instead of chiral reagent L-2.

Example 40. Synthesis of (A_P)-l,8-Diazabicyclo[5.4.0]undec-7-enium 2-N-phenoxyacetyl-5'-O-(ieributyldiphenylsilyl)-3'-O-(ieri-butyldimethylsilyl)guanosin-2'-yl 2',3'-&lt;9-bis(teri-butyldimethylsilyl)uridin-5'yl phosphorothioate [(A_P)-Mgu].

[00356] (AP)-14gu is produced as described in Example 33 using 13g instead of 13u and chiral reagent D-2 instead of chiral reagent L-2.

Scheme F

<img file="IN720DEN2012A_D0364.tif" />

(aR, 2S)-6

Βορά pyridine pyridine pyridine

HDBIT- 0

TBSO

9u

13u

1% TFA/CH₂CI₂

<img file="IN720DEN2012A_D0365.tif" />

117

Example 41. Synthesis of (Rp)-5'-O-(/eri-butyldiphenylsilyl)-3'-O-(ieri-butyldimethylsilyl)uridin-2'-yl 2',3'O-bis(teri-butyldimethylsilyl)uridin-5'-yl 77-phosphonate [(R_p)-15uu].

[00357] 13u (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). AT^V'-Bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl; 500 pmol) is added, and the mixture is stirred for 5 min. To the mixture, a solution of amino alcohol ((aR, 2S)-6) (100 pmol), which is dried by coevaportions with dry pyridine and dissolved in dry pyridine (1 mL), is added dropwise via syringe, and the mixture is stirred for 5 min under argon. Then the mixture is added via cannula into a solution of 9u (100 pmol), which is prepared by repeated coevaporations with dry pyridine and dissolution in pyridine. After 15 min, the mixture is concentrated under reduced pressure. The residue is diluted with CH2C12 (5 mL), and washed with saturated NaHCO3 (3*5 mL). The combined aqueous layers are backextracted with with CH2C12 (2*5 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to ca. 1 mL under reduced pressure. The residue is added dropwise via a syringe to a stirred 1% trifluoroacetic acid (TFA) solution in dry CH2C12 (20 mL) at 0 °C. After an additional 5 min, the mixture is diluted with dry CH2C12 (100 mL), and washed with saturated NaHCO3 aqueous solutions (2 * 100 mL). The combined aqueous layers are back-extracted with CH2C12 (2 χ 100 mL). The combined organic layers are dried over Na2SO4, filtered, and concentrated to dryness under reduced pressure to afford crude (Rp)15uu, which is analyzed by 31P NMR.

Example 42. Synthesis of (R_P)-6-.V-Benzoy 1-5'-O-(ieri-butyldiphenylsily 1)-3'-O-(ieributyldimethylsilyl)adenosin-2'-yl 2',3'-&lt;9-bis(ieri-butyldimethylsilyl)uridin-5'-yl H-phosphonate [(R_P)-15auJ. [00358] Crude (RP)-15au is produced as described in Example 41 using 13a instead of 13u.

Example 43. Synthesis of (R^^-TZ-Benzoyl-S'-OXieri-butyldiphenylsilyO-S'-O-iterrbutyldimethylsilyl)cytidin-2'-yl 2',3'-O-bis(teri-butyldimethylsilyl)uridin-5'-yl 77-phosphonate [(R_P)-15cu]. [00359] Crude (RP)-15cu is produced as described in Example 41 using 13c instead of 13u.

Example 44. Synthesis of (R_P)-2-7V-Phenoxyacetyl-5'-O-(teri-butyldiphenylsilyl)-3'-O-(ieributyldimethylsilyl)guanosin-2'-yl 2',3'-O-bis(ieri-butyldimethylsilyl)uridin-5'-yl Tf-phosphonate ](R_P)-15gu]. [00360[Crude (RP)-15gu is produced as described in Example 41 using 13g instead of 13u.

Example 45. Synthesis of (S_P)-5'-O-(teri-butyldiphenylsilyl)-3'-O-(teri-butyldimethylsilyl)uridin-2'-yl 2',3'&lt;9-bis(ieri-butyldimethylsilyl)uridin-5 '-y 177-phosphonate [(S_P)-15uu].

[00361] Crude (RP)-15uu is produced as described in Example 41 using chiral reagent (aS, 2R)-6 instead of chiral reagent (aR, 2S)-6.

Example 46. Synthesis of (S_P)-6-jV-Benzoyl-5'-O-(ieri-butyldiphenylsilyl)-3'-O-(teributyldimethylsilyl)adenosin-2'-yl 2',3'-O-bis(tert-butyldimethylsilyl)uridin-5'-yl Jf-phosphonate [(S_P)-15au], [00362JCrude (SP)-15au is produced as described in Example 41 using 13a instead of 13u and chiral reagent (aR, 2R)-6 instead of chiral reagent (aR, 2S)-6.

Example 47. Synthesis of (S_P)-4-N-Benzoyl-5'-O-(tert-butyldiphenylsilyl)-3'-O-(ieributyldimethylsilyl)cytidin-2'-yl 2',3'-O-bis(teri-butyldimethylsilyl)uridin-5'-yl /7-phosphonate [(R_P)-15cu].

118 [00363] Crude (5P)-15cu is produced as described in Example 41 using 13c instead of 13u and chiral reagent (a5, 27?)-6 instead of chiral reagent (aR, 25)-6.

Example 48. Synthesis of (5_P)-2-Ar-Phenoxyacetyl-5'-O-(ter/-butyldiphenylsilyl)-3'-O-(terrbutyldimethylsilyl)guanosin-2'-yl 2',3'-O-bis(tert-butyldimethylsilyl)uridin-5'-yl //-phosphonate [(5_P)-15gu]. [00364]Crude (5P)-15gu is produced as described in Example 41 using 13g instead of 13u and chiral reagent (aS, 2R)~6 instead of chiral reagent (aR, 25)-6.

Scheme G: Synthesis of S-acyl-2-thioethyI nucleic acid prodrugs.

<img file="IN720DEN2012A_D0366.tif" />

Example 49. Synthesis of the S-acyl-2-thioethyl nucleic acid prodrug of (/?_P)-thymidin-3'-yl thymidin-5'-yl phosphonate [(R_P)-16tt] as described in Scheme G.

[00365] (2?P)-5 '-O-(ieri-butyldiphenylsilyl)thymidin-3 '-yl 3 '-O-(reri-butyldimethylsilyl)thymidin-5 '-y 1 H phosphonate [(RP)-7tt] (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). N-chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with Sacetyl-2-thioethanol (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-l6tt.

Example 50. Synthesis of the S-acyl-2-thioethyl pronucleotide of (R_P)-6-7V-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphonate [(5p)-16at].

[00366] Crude (7?P)-16at is produced as described in Example 49 using (RP)-7at instead of (5P)-7tt. Example 51. Synthesis of the S-acyl-2-thioethyl pronucleotide of (7?_P)-4-2V-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(5p)-l 6ct].

[00367] Crude (RP)-16ct is produced as described in Example 49 using (RP)-7ct instead of (7?P)-7tt.

Example 52. Synthesis of the S-acyl-2-thioethyl pronucleotide of (5_P)-2-7V-phenoxyacetyl-deoxyguanosin3'-yl thymidin-5'-yl phosphonate [(5_P)-16gt].

[00368] Crude (RP)-16gt is produced as described in Example 49 using (7?P)-7g instead of (RP)-7tt.

119

Example 53. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)- thymidin-3'-yl thymidin-5'-yl phosphonate [(S_P)-16tt].

[00369] Crude (SP)-16tt is produced as described in Example 49 using (5P)-7tt instead of (RP)-7tt.

Example 54. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_p)-6-N-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphonate [(S_P)-16at[.

[00370] Crude (SP)-16at is produced as described in Example 49 using (SP)-7at instead of (RP)-7tt.

Example 55. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-4-AT-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(S_P)-16ct[.

[00371] Crude (SP)-16ct is produced as described in Example 49 using (&lt;SP)-7ct instead of (RP)-7tt.

Example 56. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-2-jV-phenoxyacetyl-deoxyguanosin3'-yl thymidin-5'-yl phosphonate [(S_P)-16gt[.

[00372] Crude (SP)-16gt is produced as described in Example 49 using (SP)-7gt instead of (RP)-7tt.

Example 57. Synthesis of the S-acyl-2-thioethyl pronucleotide of (R_P)-uridin-3'-yl uridin-5'-yl phosphonate [(R_P)-16uu[.

[00373] Crude (RP)-16uu is produced as described in Example 49 using (RP)-12uu instead of (RP)-7tt. Example 58. Synthesis of the S-acyl-2-thioethyl pronucleotide of (R_P)-6-jV-Benzoyl-adenosin-3'-yl uridin5'-yl phosphonate [(R_P)-16au[.

[00374] Crude (RP)-16au is produced as described in Example 49 using (RP)-12au instead of (RP)-7tt. Example 59. Synthesis of the S-acyl-2-thioethyl pronucleotide of (R_P)-4-W-Benzoyl-cytidin-3'-yl uridin-5'yl phosphonate [(R_P)-16cu[.

[00375] Crude (RP)-16cu is produced as described in Example 49 using (RP)-12cu instead of (RP)-7tt. Example 60. Synthesis of the S-acyl-2-thioethyl pronucleotide of (R_P)-2-7V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate [(R_P)-16gu[.

[00376JCrude (RP)-16gu is produced as described in Example 49 using (RP)-12gu instead of (RP)-7tt. Example 61. Synthesis of the S-acyl-2-thioethyl pronucleotide of (Sp)-uridm-3'-yl uridin-5'-yl phosphonate [(S_P)-16uu].

[00377] Crude (SP)-16uu is produced as described in Example 49 using (SP)-12uu instead of (RP)-7tt. Example 62. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-6-?7-Benzoyl-adenosin-3'-yl uridin5'-yl phosphonate [(S_P)-16au[.

[00378]Crude (SP)-16au is produced as described in Example 49 using (5P)-12au instead of (RP)-7tt. Example 63. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-4-N-Benzoyl-cytidin-3'-yl uridin-5'yl phosphonate [(S_P)-16cu[.

[00379[Crude (SP)-16cu is produced as described in Example 49 using (SP)-12au instead of (RP)-7tt. Example 64. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-2-N-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate [(S_P)-16gu[.

[00380] Crude (SP)-16gu is produced as described in Example 49 using (SP)-12gu instead of (RP)-7tt.

120

Example 65. Synthesis of the S-acyl-2-thioethyl pronucleotide of (R_P)-uridin-2'-yl uridin-5'-yl phosphonate [(2?p)-17uu].

[00381] Crude (SP)-17uu is produced as described in Example 49 using (SP)-15uu instead of (/?P)-7tt.

Example 66. Synthesis of the S-acyl-2-thioethyl pronucleotide of (/?_P)-6-2V-Benzoyl-adenosin-2'-yl uridin5'-yl phosphonate [(R_P)-17au].

[00382] Crude (/?P)-17au is produced as described in Example 49 using (SP)-15au instead of (AP)-7tt.

Example 67. Synthesis of the S-acyl-2-thioethyl pronucleotide of (/?_P)-4-2V-Benzoyl-cytidin-2'-yl uridin-5'yl phosphonate [(/?_P)-17cu[.

[00383] Crude (RP)-17cu is produced as described in Example 49 using (SP)-15cu instead of (7?P)-7tt. Example 68. Synthesis of the S-acyl-2-thioethyl pronucleotide of (7?_P)-2-jV-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl //-phosphonate [(/?_P)-17gu].

[00384]Crude (7?P)-17gu is produced as described in Example 49 using (SP)-15gu instead of (7?P)-7tt. Example 69. Synthesis of the S-acyl-2-thioethyl pronucleotide of (.S'_P)-uridin-2'-yl uridin-5'-yl phosphonate [(S_P)-17uu].

[00385] Crude (SP)-17uu is produced as described in Example 49 using (SP)-15uu instead of (/?P)-7tt.

Example 70. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-6-V-Benzoyl-adenosin-2'-yl uridin5'-yl phosphonate [(S_P)-17au[.

[00386] Crude (SP)-17au is produced as described in Example 49 using (SP)-15au instead of (RP)-7tt. Example 71. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-4-JV-Benzoyl-cytidin-2'-yl uridin-5'yl phosphonate [(S_P)-17cu].

[00387] Crude (SP)-17cu is produced as described in Example 49 using (SP)-15cu instead of (7?P)-7tt. Example 72. Synthesis of the S-acyl-2-thioethyl pronucleotide of (S_P)-2-JV-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphonate [(S_P)-17gu[.

]00388]Crude (SP)-17gu is produced as described in Example 49 using (SP)-15gu instead of (7?P)-7tt.

Scheme H: Synthesis of acyloxy pronucleotides.

<img file="IN720DEN2012A_D0367.tif" />

Example 73. Synthesis of the acyloxy pronucleotide of (7?_P)-thymidin-3'-yl thymidin-5'-yl phosphonate [(7?p)-l 8tt] as described in Scheme H.

[00389] (/?P)-5 '-O-(ieri-butyldiphenylsilyl)thymidin-3 '-y 1 3 '-O-(ieri-butyldimethylsilyl)thymidin-5 '-y 1 //phosphonate [(/?P)-7tt] (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved

121 in dry pyridine (1 mL). N-chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with hydroxymethyl acetate, (100 pmol) in dry (100 pmol) methylene chloride. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-18tt.

Example 74. Synthesis of the acyloxy pronucleotide of (R_P)-6-lV-benzoyl-deoxyadenosin-3'-yl thymidin-5'yl phosphonate [(/?_P)-18at].

[00390] Crude (RP)-18at is produced as described in Example 73 using (AP)-7at instead of (RP)-7tt. Example 75. Synthesis of the acyloxy pronucleotide of (7?_P)-4-N-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(/?_P)-18ct].

[00391] Crude (7?P)-18ct is produced as described in Example 73 using (RP)-7ct instead of (RP)-7tt. Example 76. Synthesis of the acyloxy pronucleotide of (R_P)-2-AT-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphonate [(7?_P)-18gt].

[00392] Crude (RP)-18gt is produced as described in Example 73 using (RP)-7g instead of (RP)-7tt. Example 77. Synthesis of the acyloxy pronucleotide of (S_P)- thymidin-3'-yl thymidin-5'-yl phosphonate [(S_P)-18tt].

[00393] Crude (SP)-l 8tt is produced as described in Example 73 using (SP)-7tt instead of (RP)-7tt.

Example 78. Synthesis of the acyloxy pronucleotide of (S_P)-6-N-benzoyl-deoxyadenosin-3'-yl thymidin-5'yl phosphonate [(S_P)-18at].

[00394] Crude (SP)-l 8at is produced as described in Example 73 using (5P)-7at instead of (RP)-7tt. Example 79. Synthesis of the acyloxy pronucleotide of (S_P)-4-Y-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(5_P)-18ct].

[00395] Crude (SP)-18ct is produced as described in Example 73 using (SP)-7ct instead of (RP)-7tt. Example 80. Synthesis of the acyloxy pronucleotide of (S_P)-2-A^r-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphonate [(S_P)-18gt].

[00396] Crude (SP)-l 8gt is produced as described in Example 73 using (SP)-7gt instead of (/?P)-7tt. Example 81. Synthesis of the acyloxy pronucleotide of (R_P)-uridin-3'-yl uridin-5'-yl phosphonate [(R_P)18uu].

[00397] Crude (RP)-18uu is produced as described in Example 73 using (RP)-12uu instead of (RP)-7tt. Example 82. Synthesis of the acyloxy pronucleotide of (7?_P)-6-Y-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphonate [(7?_P)-18au].

[00398] Crude (RP)-l 8au is produced as described in Example 73 using (RP)-12au instead of (7?P)-7tt.

122

Example 83. Synthesis of the acyloxy pronucleotide of (7?_P)-4-7V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphonate [(2?_P)-18cu].

[00399]Crude (7?P)-18cu is produced as described in Example 73 using (7?P)-12cu instead of (RP)-7tt.

Example 84. Synthesis of the acyloxy pronucleotide of (7?_P)-2-N-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate [(2?_p)-18gu].

[00400] Crude (7?P)-18gu is produced as described in Example 73 using (RP)-12gu instead of (7?P)-7tt. Example 85. Synthesis of the acyloxy pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphonate [(S_P)18uu].

(00401] Crude (SP)-18uu is produced as described in Example 73 using (&gt;SP)-12uu instead of (AP)-7tt. Example 86. Synthesis of the acyloxy pronucleotide of (S_P)-6-JV-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphonate [(S_P)-18au].

[00402] Crude (SP)-18au is produced as described in Example 73 using (5P)-12au instead of (2?P)-7tt. Example 87. Synthesis of the acyloxy pronucleotide of (S_P)-4-.V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphonate [(S_P)-18cu].

[00403] Crude (SP)-l 8cu is produced as described in Example 73 using (SP)-12au instead of (7?P)-7tt. Example 88. Synthesis of the acyloxy pronucleotide of (S_P)-2-AT-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate [(S_P)-18gu].

[00404] Crude (5P)-18gu is produced as described in Example 73 using (SP)-12gu instead of (7?P)-7tt. Example 89. Synthesis of the acyloxy pronucleotide of (ft_P)-uridin-2'-yl uridin-5'-yl phosphonate [(2?_P)19uu].

[00405] Crude (SP)-19uu is produced as described in Example 73 using (SP)-15uu instead of (2?P)-7tt. Example 90. Synthesis of the acyloxy pronucleotide of (R_P)-6-A-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphonate [(7?_P)-19au].

[00406] Crude (7?P)-19au is produced as described in Example 73 using (SP)-15au instead of (2?P)-7tt. Example 91. Synthesis of the acyloxy pronucleotide of (A_P)-4-jV-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphonate [(7?_P)-19cu].

[00407] Crude (RP)-19cu is produced as described in Example 73 using (SP)-15cu instead of (RP)-7tt. Example 92. Synthesis of the acyloxy pronucleotide of (7?_P)-2-/V-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphonate [(7?_P)-19guJ.

[00408] Crude (7?P)-19gu is produced as described in Example 73 using (SP)-15gu instead of (7?P)-7tt.

Example 93. Synthesis of the acyloxy pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphonate [(S_P)19uu], [00409] Crude (SP)-19uu is produced as described in Example 73 using (SP)-15uu instead of (RP)-7tt. Example 94. Synthesis of the acyloxy pronucleotide of (S_P)-6-JV-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphonate [(5_P)-19au].

[00410] Crude (5P)-19au is produced as described in Example 73 using (SP)-15au instead of (J?P)-7tt.

123

Example 95. Synthesis of the acyloxy pronucleotide of (S_P)-4-7V-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphonate [(5_P)-19cu], [00411] Crude (5P)-19cu is produced as described in Example 73 using (SP)-15cu instead of (7?P)-7tt. Example 96. Synthesis of the acyloxy pronucleotide of (5_P)-2-JV-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphonate [(S_P)-19guJ.

[00412] Crude (5P)-19gu is produced as described in Example 73 using (SP)-15gu instead of (RP)-7tt.

Scheme I: Synthesis of thioacyloxy pronucleotides.

<img file="IN720DEN2012A_D0368.tif" />

OTBS

OTBS

4tt

Et3N3HF

20tt

Example 97. Synthesis of the thioacyloxy pronucleotide of (7?_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-20tt] as described in Scheme I.

[00413](5P)-1,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(teri-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl phosphorothioate [(5P)-4tt] (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry methylene chloride (1 mL). The mixture is treated with chloromethyl acetate, prepared by the method of Bodor et al. J. Org. Chem. (1983), 48:5280, (100 pmol) in dry (100 pmol) methylene chloride. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-20tt.

Example 98. Synthesis of the thioacyloxy pronucleotide of (R_P)-6-A/-benzoyl-deoxyadenosin-3'-yl thymidin-5’-yl phosphorothioate [(R_P)-20at].

[00414] Crude (7?P)-20at is produced as described in Example 97 using (AP)-4at instead of (RP)-4tt. Example 99. Synthesis of the thioacyloxy pronucleotide of (R_P)-4-7V-benzoyl-deoxycytidin-3'-yl thymidin5'-yl phosphorothioate [(A_P)-20ctJ.

[00415] Crude (RP)-20ct is produced as described in Example 97 using (J?P)-4ct instead of (RP)-4tt.

Example 100. Synthesis of the thioacyloxy pronucleotide of (R_P)-2-JV-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-20gt].

[00416] Crude (RP)-20gt is produced as described in Example 97 using (7?P)-4g instead of (J?P)-4tt.

124

Example 101. Synthesis of the thioacyloxy pronucleotide of (Sp)- thymidin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-20tt], [00417] Crude (SP)-20tt is produced as described in Example 97 using (SP)-4tt instead of (RP)-4tt.

Example 102. Synthesis of the thioacyloxy pronucleotide of (Sp)-6-AT-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-20at].

[00418] Crude (SP)-20at is produced as described in Example 97 using (SP)-4at instead of (RP)-4tt.

Example 103. Synthesis of the thioacyloxy pronucleotide of (S_P)-4-2V-benzoyl-deoxycytidin-3'-yl thymidin5'-yl phosphorothioate [(S_P)-20ct], [00419] Crude (SP)-20ct is produced as described in Example 97 using (SP)-4ct instead of (RP)-4tt.

Example 104. Synthesis of the thioacyloxy pronucleotide of (&lt;Sp)-2-N-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(Sp)-20gt].

[00420] Crude (SP)-20gt is produced as described in Example 97 using (SP)-4gt instead of (RP)-4tt.

Example 105. Synthesis of the thioacyloxy pronucleotide of (Rp)-uridin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-20uu].

[00421] Crude (RP)-20uu is produced as described in Example 97 using (RP)-lOuu instead of (7?P)-4tt. Example 106. Synthesis of the thioacyloxy pronucleotide of (Rp)-6-N-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-20au].

[00422] Crude (RP)-20au is produced as described in Example 97 using (RP)-lOau instead of (7?P)-4tt. Example 107. Synthesis of the thioacyloxy pronucleotide of (7?_P)-4-jV-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-20cu], [00423] Crude (RP)-20cu is produced as described in Example 97 using (RP)-lOcu instead of (RP)-4tt.

Example 108. Synthesis of the thioacyloxy pronucleotide of (7?_P)-2-jV-Phenoxyacetyl-guanosin-3'-yl uridin5'-yl phosphorothioate [(7?_P)-20gu].

[00424] Crude (7?P)-20gu is produced as described in Example 97 using (RP)-lOgu instead of (RP)-4tt. Example 109. Synthesis of the thioacyloxy pronucleotide of (S’pj-uridin-S'-yl uridin-5'-yl phosphorothioate [(Sp)-20uu].

[00425] Crude (SP)-20uu is produced as described in Example 97 using (SP)-lOuu instead of (7?P)-4tt. Example 110. Synthesis of the thioacyloxy pronucleotide of (S_P)-6-jV-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-20au], [00426] Crude (SP)-20au is produced as described in Example 97 using (SP)-lOau instead of (RP)-4tt.

Example 111. Synthesis of the thioacyloxy pronucleotide of (S_P)-4-jV-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-20cu], [00427] Crude (&lt;SP)-20cu is produced as described in Example 97 using (SP)-lOau instead of (RP)-4tt.

Example 112. Synthesis of the thioacyloxy pronucleotide of (SU-Z-jV-Phenoxyacetyl-guanosin-S'-yl uridin5'-yl phosphorothioate [(S_P)-20gu].

[00428] Crude (5P)-20gu is produced as described in Example 97 using (SP)-lOgu instead of (RP)-4tt.

125

Example 113. Synthesis of the thioacyloxy pronucleotide of (R_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(R_P)-21uu].

[00429] Crude (SP)-21uu is produced as described in Example 97 using (5P)-14uu instead of (/?P)-4tt.

Example 114. Synthesis of the thioacyloxy pronucleotide of (R_P)-6-JV-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(R_P)-21au].

[00430] Crude (RP)-21au is produced as described in Example 97 using (5P)-14au instead of (5P)-4tt. Example 115. Synthesis of the thioacyloxy pronucleotide of (R_P)-4-2V-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(R_P)-21cu].

[00431]Crude (RP)-21cu is produced as described in Example 97 using (SP)-14cu instead of (2?P)-4tt.

Example 116. Synthesis of the thioacyloxy pronucleotide of (R_P)-2-JV-Phenoxyacetyl-guanosin-2'-yl uridin5'-yl phosphorothioate [(R_P)-21guJ.

[00432] Crude (2?P)-21gu is produced as described in Example 97 using (SP)-14gu instead of (7?P)-4tt.

Example 117. Synthesis of the thioacyloxy pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-21uu].

[00433] Crude (5P)-21uu is produced as described in Example 97 using (5P)-14uu instead of (RP)-4tt. Example 118. Synthesis of the thioacyloxy pronucleotide of (S_P)-6-JV-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-21au].

[00434]Crude (5P)-21au is produced as described in Example 97 using (5P)-14au instead of (RP)-4tt. Example 119. Synthesis of the thioacyloxy pronucleotide of (S_P)-4-JV-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(5_P)-21cu].

[00435] Crude (SP)-21cu is produced as described in Example 97 using (SP)-14cu instead of (RP)-4tt.

Example 120. Synthesis of the thioacyloxy pronucleotide of (5_P)-2-AT-Phenoxyacetyl-guanosin-2'-yl uridin5'-yl phosphorothioate [(5_P)-21guJ.

[00436] Crude (5P)-21gu is produced as described in Example 97 using (SP)-14gu instead of (RP)-4tt.

Scheme J: Synthesis of 2-carboalkoxyethyl pronucleotides.

<td> TBDPSQ-^q^»</td><td> n oTBOPSC^^. Meo^—</td>

<td>⁰ OTBS</td><td>^Γ OTBS</td>

<td> 4tt</td><td>^Et^^3HF_r Jr</td>

<td></td><td>²²« w</td>

Example 121. Synthesis of the 2-carboalkoxyethyl pronucleotide of (7?_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-22tt] as described in Scheme J.

[00437](SP)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(ieri-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl phosphorothioate [(5P)-4tt] (100 pmol) is dried by repeated

126 coevaporations with dry pyridine and then dissolved in dry methylene chloride (1 mL). The mixture is treated with methyl acrylate (100 pmol) in dry (100 pmol) methylene chloride. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3 x 3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (7?P)-22tt.

Example 122. Synthesis of the 2-carboalkoxyethyl pronucleotide of (7?_P)-6-N-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(2?_P)-22at[.

[00438] Crude (7?P)-22at is produced as described in Example 121 using (RP)-4at instead of (RP)-4tt. Example 123. Synthesis of the 2-carboalkoxyethyl pronucleotide of (Z?_P)-4-2V-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-22ct].

[00439] Crude (RP)-22ct is produced as described in Example 121 using (7?P)-4ct instead of (RP)-4tt. Example 124. Synthesis of the 2-carboalkoxyethyl pronucleotide of (l?_P)-2-N-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-22gt].

[00440] Crude (l?P)-22gt is produced as described in Example 121 using (2?P)-4g instead of (RP)-4tt. Example 125. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)- thymidin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-22tt[.

[00441] Crude (5P)-22tt is produced as described in Example 121 using (5P)-4tt instead of (RP)-4tt. Example 126. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-6-ALbenzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-22at[.

[00442] Crude (SP)-22at is produced as described in Example 121 using (5P)-4at instead of (RP)-4tt. Example 127. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-4-N-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(5_P)-22ct], [00443] Crude (SP)-22ct is produced as described in Example 121 using (SP)-4ct instead of (RP)-4tt. Example 128. Synthesis of the 2-carboalkoxyethyl pronucleotide of (5_P)-2-jV-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(5_P)-22gt[.

[00444] Crude (SP)-22gt is produced as described in Example 121 using (SP)-4gt instead of (7?P)-4tt.

Example 129. Synthesis of the 2-carboalkoxyethyl pronucleotide of (R_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(5_P)-22uu[.

[00445] Crude (RP)-22uu is produced as described in Example 121 using (RP)-lOuu instead of (RP)-4tt.

Example 130. Synthesis of the 2-carboalkoxyethyl pronucleotide of (R_P)-6-N-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(J?_P)-22au].

[00446] Crude (7?P)-22au is produced as described in Example 121 using (AP)-lOau instead of (7(P)-4tt.

127

Example 131. Synthesis of the 2-carboalkoxyethyl pronucleotide of (7?_P)-4-V-Benzoyl-cytidin-3'-yl uridin5'-yl phosphorothioate [(/?_P)-22cuJ.

[00447] Crude (RP)-22cu is produced as described in Example 121 using (2?P)-10cu instead of (7?P)-4tt.

Example 132. Synthesis of the 2-carboalkoxyethyl pronucleotide of (^_P)-2-V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-22guJ.

[00448] Crude (RP)-22gu is produced as described in Example 121 using (RP)-lOgu instead of (RP)-4tt. Example 133. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-22uu[.

[00449] Crude (5P)-22uu is produced as described in Example 121 using (SP)-lOuu instead of (RP)-4tt. Example 134. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-6-jV-Benzoyl-adenosin-3'-yl uridin5'-yl phosphorothioate [(S_P)-22au[.

[00450] Crude (SP)-22au is produced as described in Example 121 using (SP)-lOau instead of (AP)-4tt. Example 135. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-4-V-Benzoyl-cytidin-3'-yl uridin5'-y 1 phosphorothioate [(S_P)-22cu].

[00451]Crude (SP)-22cu is produced as described in Example 121 using (SP)-lOau instead of (7?P)-4tt.

Example 136. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-2-7V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphorothioate [(iS’p)-22gu], [00452] Crude (SP)-22gu is produced as described in Example 121 using (SP)-10gu instead of (7?P)-4tt.

Example 137. Synthesis of the 2-carboalkoxyethyl pronucleotide of (7?_P)-uridin-2'-yI uridin-5'-yl phosphorothioate [(R_P)-23uu].

[00453] Crude (SP)-23uu is produced as described in Example 121 using (5P)-14uu instead of (RP)-4tt. Example 138. Synthesis of the 2-carboalkoxyethyl pronucleotide of (/?_P)-6-N-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-23au].

[00454] Crude (7?P)-23au is produced as described in Example 121 using (SP)-14au instead of (7?P)-4tt. Example 139. Synthesis of the 2-carboalkoxyethyl pronucleotide of (7?_P)-4-jV-Benzoyl-cytidin-2'-yl uridin5'-y 1 phosphorothioate [(7?_P)-23cu].

(00455] Crude (7?P)-23cu is produced as described in Example 121 using (SP)-14cu instead of (7?P)-4tt.

Example 140. Synthesis of the 2-carboalkoxyethyl pronucleotide of (7?_P)-2-7V-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-23gu].

[00456] Crude (RP)-23gu is produced as described in Example 121 using (SP)-14gu instead of (7?P)-4tt.

Example 141. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S'_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-23uu].

[00457] Crude (SP)-23uu is produced as described in Example 121 using (SP)-14uu instead of (J?P)-4tt.

Example 142. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-6-V-Benzoyl-adenosin-2'-yl uridin5'-yl phosphorothioate [(S_P)-23auJ.

[00458] Crude (SP)-23auis produced as described in Example 121 using (SP)-14au instead of (7?P)-4tt.

128

Example 143. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-4-2V-Benzoyl-cytidin-2'-yl uridin5'-yl phosphorothioate [(S_P)-23cu].

[00459]Crude (.VP)-23cu is produced as described in Example 121 using (SP)-14cu instead of (RP)-4tt.

Example 144. Synthesis of the 2-carboalkoxyethyl pronucleotide of (S_P)-2-A/-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-23guJ.

[00460] Crude (SP)-23gu is produced as described in Example 121 using (SP)-14gu instead of (RP)-4tt.

Scheme K: Synthesis of disulfide pronucleotides.

<td> TBDPSO-i^W EtS^Et</td><td rowspan="2"> tbopso-i^b. ^c^^o~i^^c9'</td>

<td>^B0H</td>

<td> OTBS 4tt</td><td> OTBS</td>

<td> EtjN3HF</td><td></td>

Example 145. Synthesis of the disulfide pronucleotide of (R_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-24tt] as described in Scheme K.

[00461](SP)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(teri-butyldiphenylsilyl)thymidin-3'-yl 3'-&lt;9-(teributyldimethylsilyl)thymidin-5'-yl phosphorothioate [(SP)-4tt[ (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry ethanol (1 mL). The mixture is treated with diethyl disulfide (200 pmol) in dry (100 pmol) ethanol. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3 χ 3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-24tt.

Example 146. Synthesis of the disulfide pronucleotide of (R_P)-6-N-benzoyl-deoxyadenosin-3'-yl thymidin5'-yl phosphorothioate [(R_P)-24at[.

[00462] Crude (RP)-24at is produced as described in Example 145 using (RP)-4at instead of (RP)-4tt.

Example 147. Synthesis of the disulfide pronucleotide of (R_P)-4-7V-benzoyl-deoxycytidin-3'-yl thymidin-5'yl phosphorothioate [(R_P)-24ct[.

[00463] Crude (RP)-24ct is produced as described in Example 145 using (RP)-4ct instead of (RP)-4tt.

Example 148. Synthesis of the disulfide pronucleotide of (R_P)-2W-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-24gt], [00464] Crude (RP)-24gt is produced as described in Example 145 using (RP)-4g instead of (RP)-4tt.

129

Example 149. Synthesis of the disulfide pronucleotide of (5_P)- thymidin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-24tt).

[00465] Crude (SP)-24tt is produced as described in Example 145 using (SP)-4tt instead of (RP)-4tt.

Example 150. Synthesis of the disulfide pronucleotide of (S_P)-6-jV-benzoyl-deoxyadenosin-3'-yl thymidin5'-yl phosphorothioate [(S_P)-24at].

[00466] Crude (SP)-24at is produced as described in Example 145 using (SP)-4at instead of (RP)-4tt.

Example 151. Synthesis of the disulfide pronucleotide of (S_P)-4-2V-benzoyl-deoxycytidin-3'-yl thymidin-5'yl phosphorothioate [(S_P)-24ct].

[00467] Crude (SP)-24ct is produced as described in Example 145 using (SP)-4ct instead of (RP)-4tt. Example 152. Synthesis of the disulfide pronucleotide of (S_P)-2-N-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(S_p)-24gtJ.

[00468] Crude (SP)-24gt is produced as described in Example 145 using (SP)-4gt instead of (RP)-4tt. Example 153. Synthesis of the disulfide pronucleotide of (R_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-24uu].

[00469] Crude (RP)-24uu is produced as described in Example 145 using (RP)-lOuu instead of (RP)-4tt. Example 154. Synthesis of the disulfide pronucleotide of (R_P)-6-A-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-24au].

[00470] Crude (RP)-24au is produced as described in Example 145 using (RP)-lOau instead of (RP)-4tt. Example 155. Synthesis of the disulfide pronucleotide of (R_P)-4-N-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate f(R_P)-24cu].

[00471]Crude (RP)-24cu is produced as described in Example 145 using (RP)-lOcu instead of (RP)-4tt. Example 156. Synthesis of the disulfide pronucleotide of (R_P)-2-N-Phenoxyacetyl-guanosin-3'-yl uridin-5'yl phosphorothioate [(R_P)-24gu].

[00472] Crude (RP)-24gu is produced as described in Example 145 using (RP)-lOgu instead of (RP)-4tt. Example 157. Synthesis of the disulfide pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-24uu].

[00473] Crude (5P)-24uu is produced as described in Example 145 using (SP)-lOuu instead of (RP)-4tt. Example 158. Synthesis of the disulfide pronucleotide of (S_P)-6-A/-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-24au].

[00474] Crude (5P)-24au is produced as described in Example 145 using (SP)-lOau instead of (RP)-4tt.

Example 159. Synthesis of the disulfide pronucleotide of (S_P)-4-JV-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-24cu].

[00475] Crude (SP)-24cu is produced as described in Example 145 using (SP)-lOau instead of (RP)-4tt.

Example 160. Synthesis of the disulfide pronucleotide of (S_P)-2-7V-Phenoxyacetyl-guanosin-3'-yl uridin-5'yl phosphorothioate [(S_P)-24gu].

[00476] Crude (SP)-24gu is produced as described in Example 145 using (SP)-lOgu instead of (RP)-4tt.

130

Example 161. Synthesis of the disulfide pronucleotide of (7?_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(.R_P)-25uu].

[00477] Crude (SP)-25uu is produced as described in Example 145 using (SP)-14uu instead of (7?P)-4tt.

Example 162. Synthesis of the disulfide pronucleotide of (R_P)-6-AT-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-25au].

[00478] Crude (RP)-25au is produced as described in Example 145 using (SP)-14au instead of (RP)-4tt. Example 163. Synthesis of the disulfide pronucleotide of (A_P)-4-jV-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(J?_P)-25cu], [00479] Crude (RP)-25cu is produced as described in Example 145 using (SP)-14cu instead of (RP)-4tt. Example 164. Synthesis of the disulfide pronucleotide of (7?_P)-2-jV-Phenoxyacetyl-guanosin-2'-yl uridin-5'yl phosphorothioate [(R_P)-25gu].

[00480] Crude (RP)-25gu is produced as described in Example 145 using (SP)-14gu instead of (RP)-4tt. Example 165. Synthesis of the disulfide pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-25uu[.

[00481] Crude (SP)-25uu is produced as described in Example 145 using (SP)-14uu instead of (7?P)-4tt. Example 166. Synthesis of the disulfide pronucleotide of (S_P)-6-7V-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-25au].

[00482] Crude (SP)-25au is produced as described in Example 145 using (SP)-14au instead of (7?P)-4tt. Example 167. Synthesis of the disulfide pronucleotide of (S_P)-4-2V-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-25cu], [00483] Crude (SP)-25cu is produced as described in Example 145 using (SP)-14cu instead of (/?P)-4tt. Example 168. Synthesis of the disulfide pronucleotide of (S_P)-2-2V-Phenoxyacetyl-guanosin-2'-yl uridin-5'yl phosphorothioate [(S_P)-25gu].

[00484] Crude (SP)-25gu is produced as described in Example 145 using (SP)-14gu instead of (RP)-4tt.

Scheme L: Synthesis of thioacetal pronucleotides.

<img file="IN720DEN2012A_D0369.tif" />

Example 169. Synthesis of the thioacetal pronucleotide of (/?_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-26tt] as described in Scheme L.

[00485] 3,3-Dimethoxypropyl acetate (100 pmol) is added to a solution of trimethylsilyltriflate (100 pmol) in methylene chloride (1 mL) at -78 °C. After stirring at -78 °C for 30 min, (5P)-1,8131

Diazabicyclo[5.4.0]undec-7-enium 5 '-0-(terrbutyldiphenylsilyl)thymidin-3 '-y 1 3 '-O-(ter/butyldimethylsilyl)thymidin-5'-yl phosphorothioate [(SP)-4tt] (100 pmol) is added in dry methylene chloride (1 mL). The mixture is allowed to slowly warm to room temperature. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydro fluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-26tt.

Example 170. Synthesis of the thioacetal pronucleotide of (R_P)-6-79-benzoyl-deoxyadenosin-3'-yl thymidin5’-yl phosphorothioate [(R_P)-26at], [00486] Crude (RP)-26at is produced as described in Example 169 using (RP)-4at instead of (RP)-4tt. Example 171. Synthesis of the thioacetal pronucleotide of (R_P)-4-7V-benzoyl-deoxycytidin-3'-yl thymidin-5'yl phosphorothioate [(R_P)-26ct], [00487] Crude (RP)-26ct is produced as described in Example 169 using (RP)-4ct instead of (RP)-4tt. Example 172. Synthesis of the thioacetal pronucleotide of (R_P)-2-jV-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-26gt], [00488] Crude (RP)-26gt is produced as described in Example 169 using (RP)-4g instead of (RP)-4tt. Example 173. Synthesis of the thioacetal pronucleotide of (R_P)- thymidin-3'-yl thymidin-5'-yl phosphorothioate ](S_P)-26tt].

[00489] Crude (SP)-26tt is produced as described in Example 169 using (SP)-4tt instead of (RP)-4tt. Example 174. Synthesis of the thioacetal pronucleotide of (R_P)-6-2V-benzoyl-deoxyadenosin-3'-yl thymidin5'-yl phosphorothioate ](S_P)-26at].

[00490] Crude (SP)-26at is produced as described in Example 169 using (SP)-4at instead of (RP)-4tt. Example 175. Synthesis of the thioacetal pronucleotide of (S_P)-4-jV-benzoyl-deoxycytidin-3'-yl thymidin-5'yl phosphorothioate [(R_P)-26ct].

[00491] Crude (SP)-26ct is produced as described in Example 169 using (RP)-4ct instead of (RP)-4tt. Example 176. Synthesis of the thioacetal pronucleotide of (R_P)-2-AT-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-26gt].

[00492] Crude (SP)-26gt is produced as described in Example 169 using (RP)-4gt instead of (RP)-4tt. Example 177. Synthesis of the thioacetal pronucleotide of (R_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-26uu].

[00493] Crude (RP)-26uu is produced as described in Example 169 using (RP)-lOuu instead of (RP)-4tt. Example 178. Synthesis of the thioacetal pronucleotide of (R_P)-6-jV-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-26au].

[00494] Crude (RP)-26au is produced as described in Example 169 using (RP)-lOau instead of (RP)-4tt.

132

Example 179. Synthesis of the thioacetal pronucleotide of (A_P)-4-N-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(A_P)-26cu].

[00495] Crude (AP)-26cu is produced as described in Example 169 using (AP)-lOcu instead of (AP)-4tt.

Example 180. Synthesis of the thioacetal pronucleotide of (7?_P)-2-AT-Phenoxyacetyl-guanosin-3'-yl uridin-5'yl phosphorothioate [(A_P)-26guJ.

[00496] Crude (AP)-26gu is produced as described in Example 169 using (RP)-lOgu instead of (AP)-4tt. Example 181. Synthesis of the thioacetal pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(5_P)-26uu].

[00497] Crude (5P)-26uu is produced as described in Example 169 using (SP)-lOuu instead of (AP)-4tt. Example 182. Synthesis of the thioacetal pronucleotide of (S_P)-6-jV-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-26auJ.

[00498] Crude (SP)-26au is produced as described in Example 169 using (SP)-lOau instead of (AP)-4tt. Example 183. Synthesis of the thioacetal pronucleotide of (S_P)-4-7V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-26cu].

[00499]Crude (SP)-26cu is produced as described in Example 169 using (5P)-10au instead of (AP)-4tt.

Example 184. Synthesis of the thioacetal pronucleotide of (S_P)-24V-Phenoxyacetyl-guanosin-3'-yl uridin-5’yl phosphorothioate [(S_P)-26guJ.

[00500] Crude (SP)-26gu is produced as described in Example 169 using (SP)-lOgu instead of (AP)-4tt. Example 185. Synthesis of the thioacetal pronucleotide of (A_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(A_P)-27uuJ.

[00501]Crude (5P)-27uu is produced as described in Example 169 using (SP)-14uu instead of (AP)-4tt. Example 186. Synthesis of the thioacetal pronucleotide of (A_P)-6-jV-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(A_P)-27au].

[00502] Crude (AP)-27au is produced as described in Example 169 using (5P)-14au instead of (RP)-4tt. Example 187. Synthesis of the thioacetal pronucleotide of (R_P)-44V-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(A_P)-27cuJ.

[00503] Crude (RP)-27cu is produced as described in Example 169 using (SP)-14cu instead of (AP)-4tt.

Example 188. Synthesis of the thioacetal pronucleotide of (A_P)-2-N-Phenoxyacetyl-guanosin-2'-yl uridin-5'yl phosphorothioate [(A_P)-27gu].

[00504] Crude (AP)-27gu is produced as described in Example 169 using (5P)-14gu instead of (AP)-4tt. Example 189. Synthesis of the thioacetal pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-27uuJ.

[00505] Crude (5P)-27uu is produced as described in Example 169 using (SP)-14uu instead of (AP)-4tt. Example 190. Synthesis of the thioacetal pronucleotide of (5_P)-6-7V-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-27au].

[00506] Crude (SP)-27au is produced as described in Example 169 using (SP)-14au instead of (AP)-4tt.

133

Example 191. Synthesis of the thioacetal pronucleotide of (S_P)-4-jV-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(5_P)-27cu], [00507] Crude (SP)-27cu is produced as described in Example 169 using (5P)-14cu instead of (7?P)-4tt. Example 192. Synthesis of the thioacetal pronucleotide of (5_P)-2-jV-Phenoxyacetyl-guanosin-2’-yl uridin-5'yl phosphorothioate [(S_P)-27gu], [00508] Crude (SP)-27gu is produced as described in Example 169 using (5P)-14gu instead of (AP)-4tt.

Scheme M: Synthesis of C3 enol ester pronucleotides.

<img file="IN720DEN2012A_D0370.tif" />

OTBS

DMF

<img file="IN720DEN2012A_D0371.tif" />

Example 193. Synthesis of the C3 enol ester pronucleotide of (R_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(J?_P)-28tt] as described in Scheme M.

[00509]To a solution of (E)-3-chloroprop-1-enyl acetate (100 pmol) in DMF (1 mL) is added (SP)-1,8Diazabicyclo[5.4.0]undec-7-enium 5 '-O-(iert-butyldiphenylsily l)thymidin-3 '-yl 3 '-O-(terN butyldimethylsilyl)thymidin-5'-yl phosphorothioate [(5P)-4tt] (100 pmol). After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3*3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-28tt.

Example 194. Synthesis of the C3 enol ester pronucleotide of (??_P)-6-2V-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-28at], [00510] Crude (RP)-28at is produced as described in Example 193 using (5P)-4at instead of (5P)-4tt. Example 195. Synthesis of the C3 enol ester pronucleotide of (J?_P)-4-V-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(2?_P)-28ct].

[00511]Crude (7?P)-28ct is produced as described in Example 193 using (5P)-4ct instead of (7?P)-4tt. Example 196. Synthesis of the C3 enol ester pronucleotide of (J?_P)-2-A^r-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-28gt].

[00512] Crude (RP)-28gt is produced as described in Example 193 using (7?P)-4g instead of (7?P)-4tt. Example 197. Synthesis of the C3 enol ester pronucleotide of (S_P)- thymidin-3'-yl thymidin-5'-yl phosphorothioate [(5_P)-28tt[.

134 [00513] Crude (5P)-28tt is produced as described in Example 193 using (SP)-4tt instead of (RP)-4tt.

Example 198. Synthesis of the C3 enol ester pronucleotide of (S_P)-6-jV-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-28at].

[00514] Crude (SP)-28at is produced as described in Example 193 using (SP)-4at instead of (RP)-4tt. Example 199. Synthesis of the C3 enol ester pronucleotide of (S_P)-4-JV-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-28ct[.

[00515] Crude (SP)-28ct is produced as described in Example 193 using (5P)-4ct instead of (2tP)-4tt. Example 200. Synthesis of the C3 enol ester pronucleotide of (S_P)-2-jV-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-28gt[.

[00516] Crude (SP)-28gt is produced as described in Example 193 using (SP)-4gt instead of (7?P)-4tt. Example 201. Synthesis of the C3 enol ester pronucleotide of (2?_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-28uu], [00517] Crude (7?P)-28uu is produced as described in Example 193 using (2?P)-10uu instead of (7?P)-4tt. Example 202. Synthesis of the C3 enol ester pronucleotide of (J?_P)-6-7V-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-28au], [00518] Crude (RP)-28au is produced as described in Example 193 using (7?P)-10au instead of (RP)-4tt. Example 203. Synthesis of the C3 enol ester pronucleotide of (jR_P)-4-AT-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(J?_P)-28cu].

[00519]Crude (7?P)-28cu is produced as described in Example 193 using (J?P)-10cu instead of (RP)-4tt. Example 204. Synthesis of the C3 enol ester pronucleotide of (J?_P)-2-2V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-28gu[.

[00520]Crude (RP)-28gu is produced as described in Example 193 using (RP)-lOgu instead of (RP)-4tt. Example 205. Synthesis of the C3 enol ester pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-28uuJ.

[00521] Crude (SP)-28uu is produced as described in Example 193 using (SP)-lOuu instead of (AP)-4tt. Example 206. Synthesis of the C3 enol ester pronucleotide of (S_P)-6-?V-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-28au].

[00522] Crude (SP)-28au is produced as described in Example 193 using (SP)-lOau instead of (RP)-4tt. Example 207. Synthesis of the C3 enol ester pronucleotide of (S_P)-4-7V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-28cuJ.

[00523] Crude (SP)-28cu is produced as described in Example 193 using (SP)-lOau instead of (RP)-4tt. Example 208. Synthesis of the C3 enol ester pronucleotide of (S_P)-2-N-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphorothioate [(Sp)-28gu].

[00524]Crude (SP)-28gu is produced as described in Example 193 using (SP)-lOgu instead of (7?P)-4tt. Example 209. Synthesis of the C3 enol ester pronucleotide of (R_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(/?_P)-29uu[.

135 [00525] Crude (SP)-29uu is produced as described in Example 193 using (SP)-14uu instead of (J?P)-4tt.

Example 210. Synthesis of the C3 enol ester pronucleotide of (R_P)-6-N-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-29au], [00526] Crude (7?P)-29au is produced as described in Example 193 using (SP)-14au instead of (RP)-4tt. Example 211. Synthesis of the C3 enol ester pronucleotide of (7?_P)-4-jV-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-29cu].

[00527] Crude (RP)-29cu is produced as described in Example 193 using (SP)-14cu instead of (RP)-4tt. Example 212. Synthesis of the C3 enol ester pronucleotide of (A_P)-2-jV-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphorothioate ](7?_P)-29gu].

[00528] Crude (7?P)-29gu is produced as described in Example 193 using (SP)-14gu instead of (7?P)-4tt. Example 213. Synthesis of the C3 enol ester pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-29uu], [00529] Crude (SP)-29uu is produced as described in Example 193 using (SP)-14uu instead of (RP)-4tt. Example 214. Synthesis of the C3 enol ester pronucleotide of (S_P)-6-7V-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-29au].

[00530] Crude (SP)-29au is produced as described in Example 193 using (SP)-14au instead of (RP)-4tt. Example 215. Synthesis of the C3 enol ester pronucleotide of (S_P)-4-7V-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate ](S_P)-29cu].

[00531] Crude (SP)-29cu is produced as described in Example 193 using (SP)-14cu instead of (2?P)-4tt. Example 216. Synthesis of the C3 enol ester pronucleotide of (S_P)-2-jV-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphorothioate ](S’_P)-29gu].

[00532] Crude (SP)-29gu is produced as described in Example 193 using (SP)-14gu instead of (RP)-4tt.

Scheme N: Synthesis of C4 enol ester pronucleotides.

<img file="IN720DEN2012A_D0372.tif" />

OTBS

<img file="IN720DEN2012A_D0373.tif" />

Example 217. Synthesis of the C4 enol ester pronucleotide of (2?_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-30tt] as described in Scheme N.

[00533] To a solution of (E)-4-chlorobut-l-enyl acetate (100 pmol) in DMF (1 mL) is added (SP)-1,8Diazabicyclo[5.4.0]undec-7-enium 5'-&lt;9-(iert-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl phosphorothioate [(SP)-4tt] (100 pmol). After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at

136 room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3*3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (7?P)-30tt.

Example 218. Synthesis of the C4 enol ester pronucleotide of (2?_P)-6-N-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(jR_P)-30at].

(00534] Crude (RP)-30at is produced as described in Example 217 using (RP)-4at instead of (7?P)-4tt. Example 219. Synthesis of the C4 enol ester pronucleotide of (R_P)-4-AT-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-30ctJ.

[00535] Crude (7?P)-30ct is produced as described in Example 217 using (7?P)-4ct instead of (RP)-4tt. Example 220. Synthesis of the C4 enol ester pronucleotide of (7?_P)-2-lV-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-30gt].

(00536] Crude (AP)-30gt is produced as described in Example 217 using (7?P)-4g instead of (RP)-4tt. Example 221. Synthesis of the C4 enol ester pronucleotide of (S_P)- thymidin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-30tt], [00537] Crude (SP)-30tt is produced as described in Example 217 using (SP)-4tt instead of (7?P)-4tt. Example 222. Synthesis of the C4 enol ester pronucleotide of (S_P)-6-AT-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-30at].

(00538] Crude (SP)-30at is produced as described in Example 217 using (SP)-4at instead of (7?P)-4tt. Example 223. Synthesis of the C4 enol ester pronucleotide of (5_P)-4-jV-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-30ct], [00539] Crude (SP)-30ct is produced as described in Example 217 using (SP)-4ct instead of (RP)-4tt. Example 224. Synthesis of the C4 enol ester pronucleotide of (S_P)-2-N-phenoxyacetyl-deoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-30gt], [00540] Crude (SP)-30gt is produced as described in Example 217 using (SP)-4gt instead of (RP)-4tt. Example 225. Synthesis of the C4 enol ester pronucleotide of (7?_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-30uu], [00541]Crude (7?P)-30uu is produced as described in Example 217 using (RP)-lOuu instead of (RP)-4tt. Example 226. Synthesis of the C4 enol ester pronucleotide of (2?_P)-6-7V-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-30au].

[00542] Crude (RP)-30au is produced as described in Example 217 using (RP)-lOau instead of (7?P)-4tt. Example 227. Synthesis of the C4 enol ester pronucleotide of (2?_P)-4-lV-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(l?_P)-30cu].

[00543] Crude (RP)-30cu is produced as described in Example 217 using (RP)-lOcu instead of (2?P)-4tt.

137

Example 228. Synthesis of the C4 enol ester pronucleotide of (7?_P)-2-N-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-30gu].

[00544] Crude (7?P)-30gu is produced as described in Example 217 using (RP)-lOgu instead of (RP)-4tt.

Example 229. Synthesis of the C4 enol ester pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-30uu].

[00545] Crude (5P)-30uu is produced as described in Example 217 using (SP)-lOuu instead of (7?P)-4tt. Example 230. Synthesis of the C4 enol ester pronucleotide of (S_P)-6-JV-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-30au], [00546]Crude (SP)-30au is produced as described in Example 217 using (SP)-lOau instead of (7?P)-4tt. Example 231. Synthesis of the C4 enol ester pronucleotide of (5_P)-4-jV-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(5_P)-30cu].

[00547] Crude (SP)-30cu is produced as described in Example 217 using (SP)-lOau instead of (RP)-4tt. Example 232. Synthesis of the C4 enol ester pronucleotide of (S_P)-2-7V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-30gu], [00548]Crude (SP)-30gu is produced as described in Example 217 using (SP)-lOgu instead of (7?P)-4tt.

Example 233. Synthesis of the C4 enol ester pronucleotide of (7?_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-31uu].

[00549] Crude (5P)-31uu is produced as described in Example 217 using (SP)-14uu instead of (RP)-4tt. Example 234. Synthesis of the C4 enol ester pronucleotide of (2?_P)-6-N-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-31au], [00550] Crude (7?P)-3 lau is produced as described in Example 217 using (SP)-14au instead of (7?P)-4tt. Example 235. Synthesis of the C4 enol ester pronucleotide of (R_P)-4-AT-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(2?_P)-31cu].

[00551] Crude (7?P)-31 cu is produced as described in Example 217 using (SP)-14cu instead of (RP)-4tt. Example 236. Synthesis of the C4 enol ester pronucleotide of (S_P)-2-N-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphorothioate [(R_P)-31gu], [00552] Crude (7?P)-31gu is produced as described in Example 217 using (SP)-14gu instead of (7?P)-4tt. Example 237. Synthesis of the C4 enol ester pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-31uu].

[00553]Crude (SP)-31uu is produced as described in Example 217 using (SP)-14uu instead of (RP)-4tt.

Example 238. Synthesis of the C4 enol ester pronucleotide of (S_P)-6-jV-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-31au].

[00554] Crude (SP)-31au is produced as described in Example 217 using (SP)-14au instead of (RP)-4tt. Example 239. Synthesis of the C4 enol ester pronucleotide of (S_P)-4-7V-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-31cu].

[00555] Crude (SP)-31cu is produced as described in Example 217 using (SP)-14cu instead of (l?P)-4tt.

138

Example 240. Synthesis of the C4 enol ester pronucleotide of (S_P)-2-Y-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphorothioate [(.^)-3 lgu].

[00556] Crude (5P)-31gu is produced as described in Example 217 using (SP)-14gu instead of (RP)-4tt.

Scheme O: Synthesis of protected 2’-5’-A₃ H-phosphonate (ΜβΟΟ_βΗ4)₃(\

<img file="IN720DEN2012A_D0374.tif" />

TBSO Ckp^O HDBlT- O' Ή

<img file="IN720DEN2012A_D0375.tif" />

9a

TFA

<img file="IN720DEN2012A_D0376.tif" />

TBSO OTBS

Example 241. The synthesis of a 5’-O-(methoxytrityl) protected 2’-5’-A₃ H-phosphonate is illustrated in

Scheme O-a.

[00557] 5’-O-(Methoxytrityl) protected compound 32 is coupled with 9a as described in Scheme 6, Example 41. The resulting H-phosphonate 33 is subjected to 5’-O-(methoxytrityl) deprotection by treatment with 1% TFA in CH2C12 to give 5’-OH compound 34. Coupling of 34 with 32, as described in Scheme 6, Example 41, gives the H-phosphonate trinucleotide 35. Deprotection of the 5’-OH group with 1% TFA in CH2C12 to gives H-phosphonate trinucleotide 36.

139

Scheme O-b: Synthesis of a 2’-5’-A₃ S-acetyl-2-thioethyl pronucleotide

A

NCS

CHsCN pyridine

<img file="IN720DEN2012A_D0377.tif" />

TBSO

TBSO

OTB S

A

<img file="IN720DEN2012A_D0378.tif" />

Example 242. Synthesis a 2’-5’-A3 S-acetyl-2-thioethyl pronucleotide is illustrated in Scheme O-b.

[00558] 5’-OH H-phosphonate trinucleotide compound 36 is converted to the S-acetyl-2-thioethyl prodrug by the method of Eldrup, as described in US 7,202,224. To 36 (1 mmol) is added ΙΗ-tetrazole (1.1 mmol) and the mixture dried overnight over P2O5. To this mixture is added dry acetonitrile (10 mL) followed by bis(Sacetyl-2-thioethyl)N,N-diisopropylphosphoramidite (1.1 mmol) and the resulting mixture is stirred at room temperature for 2 hours. The solvent is removed, the residue cooled to -40 °C and a solution of m-CPBA (1.0 mmol) in dichloromethane (10 mL) is added. After stining at room temperature for 1 hour, aq. NaHSO3 is added and the organic layer separated and product 37 is isolated by chromatography.

[00559] Compound 37 is converted into the final product 39 following the procedure of Scheme 7,

Example 49. Compound 37 (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). ΛΓ-chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with S-acetyl-2-thioethanol (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford 39.

140

Scheme P: Synthesis of trimethylammoniumethyl nucleic acid prodrugs.

<img file="IN720DEN2012A_D0379.tif" />

7tt

N3S

CHaCN

<img file="IN720DEN2012A_D0380.tif" />

pyridne

<img file="IN720DEN2012A_D0381.tif" />

OTBS

Bght3HF

<img file="IN720DEN2012A_D0382.tif" />

Example 243. Synthesis of the trimethylammoniumethyl nucleic acid prodrug of (/?_P)-thymidin-3'-yl thymidin-5'-yl phosphonate [(R_P)-16tt] as described in Scheme P.

[00560] (2?P)-5 '-O-(feri-butyldiphenylsilyl)thymidin-3 '-yl 3 '-O-(ieri-butyldimethylsilyl)thymidin-5 '-y 1 H phosphonate [(/?P)-7tt] (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). JV-chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with 1 (2-hydroxy)-ethyl-trimethylammonium chloride (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (/?P)-40tt.

Example 244. Synthesis of the trimethylammoniumethyl pronucleotide of (R_P)-6-JV-benzoyldeoxyadenosin-3'-yl thymidin-5'-yl phosphonate [(R_P)-40at[.

[00561] Crude (RP)-40at is produced as described in Example 243 using (RP)-7at instead of (7?P)-7tt. Example 245. Synthesis of the trimethylammoniumethyl pronucleotide of (2?_P)-4-jV-benzoyl-deoxycytidin3 '-yl thymidin-5'-yl phosphonate [(R_P)-40ct].

[00562] Crude (RP)-40ct is produced as described in Example 49 using (RP)-7ct instead of (RP)-7tt. Example 246. Synthesis of the trimethylammoniumethyl pronucleotide of (/?_P)-2-JV-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphonate [(Z?_P)-40gt].

[00563] Crude (AP)-40gt is produced as described in Example 49 using (RP)-7g instead of (RP)-7tt.

Example 247. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)- thymidin-3'-yl thymidin-5'yl phosphonate [(5_P)-40tt].

[00564] Crude (5P)-40tt is produced as described in Example 49 using (5P)-7tt instead of (7?P)-7tt.

141

Example 248. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-6-?V-benzoyl-deoxyadenosin3'-yl thymidin-5'-yl phosphonate [(S_P)-40at], [00565] Crude (SP)-40at is produced as described in Example 49 using (SP)-7at instead of (7?P)-7tt.

Example 249. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-4-7/-benzoyl-deoxycytidin3'-yl thymidin-5'-yl phosphonate [(S_P)-40ctJ.

[00566] Crude (SP)-40ct is produced as described in Example 49 using (SP)-7ct instead of (7?P)-7tt. Example 250. Synthesis of the trimethylammoniumethyl pronucleotide of (S'_P)-2-jV-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphonate [(S_P)-40gt], ]00567] Crude (SP)-40gt is produced as described in Example 49 using (5P)-7gt instead of (RP)-7tt. Example 251. Synthesis of the trimethylammoniumethyl pronucleotide of (Rp)-uridin-3'-yl uridin-5'-yl phosphonate [(R_P)-40uuJ.

[00568] Crude (7?P)-40uu is produced as described in Example 49 using (RP)-12uu instead of (RP)-7tt.

Example 252. Synthesis of the trimethylammoniumethyl pronucleotide of (7?_P)-6-AT-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphonate [(R_P)-40au].

[00569] Crude (RP)-40au is produced as described in Example 49 using (RP)-12au instead of (RP)-7tt. Example 253. Synthesis of the trimethylammoniumethyl pronucleotide of (7?_P)-4-?/-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphonate [(R_P)-40cu].

[00570] Crude (7?P)-16cu is produced as described in Example 49 using (RP)-12cu instead of (RP)-7tt. Example 254. Synthesis of the trimethylammoniumethyl pronucleotide of (R_P)-2-7V-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphonate [(R_P)-40gu], [00571] Crude (RP)-40gu is produced as described in Example 49 using (RP)-12gu instead of (7?P)-7tt. Example 255. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphonate [(S_P)-40uu], [00572] Crude (&lt;SP)-40uu is produced as described in Example 49 using (SP)-12uu instead of (J?P)-7tt. Example 256. Synthesis of the trimethylammoniumethyl pronucleotide of (Si&gt;)-6-JV-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphonate [(S_P)-40au].

[00573] Crude (SP)-40au is produced as described in Example 49 using (SP)-12au instead of (RP)-7tt. Example 257. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-4-/V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphonate [(S_P)-40cu].

[00574] Crude (SP)-40cu is produced as described in Example 49 using (SP)-12au instead of (7?P)-7tt. Example 258. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-2-?/-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphonate [(S_P)-40gu], [00575] Crude (SP)-40gu is produced as described in Example 49 using (SP)-12gu instead of (7?P)-7tt. Example 259. Synthesis of the trimethylammoniumethyl pronucleotide of (R_P)-uridin-2'-yl uridin-5'-yl phosphonate [(R_P)-41uu], [00576] Crude (RP)-41uu is produced as described in Example 49 using (7?P)-15uu instead of (RP)-7tt.

142

Example 260. Synthesis of the trimethylammoniumethyl pronucleotide of (R_p)-6-jV-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphonate [(R_P)-41au], [00577] Crude (RP)-41au is produced as described in Example 49 using (SP)-15au instead of (RP)-7tt.

Example 261. Synthesis of the trimethylammoniumethyl pronucleotide of (R_P)-4-jV-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphonate [(R_P)-41cu], [00578] Crude (RP)-41cu is produced as described in Example 49 using (SP)-15cu instead of (RP)-7tt. Example 262. Synthesis of the trimethylammoniumethyl pronucleotide of (R_P)-2-jV-Phenoxyacetylguanosin-2'-yl uridin-5'-yl H-phosphonate [(R_P)-41guJ.

[00579] Crude (RP)-41gu is produced as described in Example 49 using (SP)-15gu instead of (RP)-7tt. Example 263. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphonate [(5^)-4 luu].

[00580] Crude (SP)-41uu is produced as described in Example 49 using (SP)-15uu instead of (RP)-7tt.

Example 264. Synthesis of the trimethylammoniumethyl pronucleotide of (5_P)-6-jV-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphonate [(5_P)-41au].

[00581] Crude (SP)-41au is produced as described in Example 49 using (SP)-15au instead of (RP)-7tt. Example 265. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-4-W-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphonate [(S_P)-41cu].

[00582]Crude (SP)-41cu is produced as described in Example 49 using (SP)-15cu instead of (RP)-7tt. Example 266. Synthesis of the trimethylammoniumethyl pronucleotide of (S_P)-2-jV-Phenoxyacetylguanosin-2'-yl uridin-5'-yl phosphonate [(S_p)-4lgu].

[00583] Crude (&lt;SP)-41gu is produced as described in Example 49 using (5P)-15gu instead of (RP)-7tt.

Scheme Q: Synthesis of alkylhydroxamate nucleic acid prodrugs.

<img file="IN720DEN2012A_D0383.tif" />

<img file="IN720DEN2012A_D0384.tif" />

Example 267. Synthesis of the alkylhydroxamate nucleic acid prodrug of (R_P)-thymidin-3'-yl thymidin-5'yl phosphonate [(R_P)-42tt] as described in Scheme Q.

[00584] (RP)-5 '-O-(ieri-butyldiphenylsilyl)thymidin-3 '-yl 3 '-O-(iert-butyldimethylsilyl)thymidin-5 '-yl Hphosphonate [(RP)-7tt] (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved 143 in dry pyridine (1 mL). JV-chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with Nmethoxy-N-methyl-3-hydroxypropionamide (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-42tt.

Example 268. Synthesis of the alkylhydroxamate pronucleotide of (J?_P)-6-N-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphonate [(7?_P)-42at].

[00585] Crude (7?P)-42at is produced as described in Example 267 using (RP)-7at instead of (7?P)-7tt. Example 269. Synthesis of the alkylhydroxamate pronucleotide of (2?_P)-4-7V-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(7?_P)-42ct].

[00586] Crude (7?P)-42ct is produced as described in Example 267 using (2?P)-7ct instead of (l?P)-7tt. Example 270. Synthesis of the alkylhydroxamate pronucleotide of (2?_P)-2-W-phenoxyacetyl-deoxyguanosin3’-yl thymidin-5’-yl phosphonate [(R_P)-42gtJ.

[00587] Crude (RP)-42gt is produced as described in Example 267 using (7?P)-7g instead of (7?P)-7tt. Example 271. Synthesis of the alkylhydroxamate pronucleotide of (S_P)- thymidin-3'-yl thymidin-5'-yl phosphonate [(S_P)-42tt[.

[00588] Crude (SP)-42tt is produced as described in Example 267 using (5P)-7tt instead of (RP)-7tt. Example 272. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-6-N-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphonate [(S_P)-42at[.

[00589] Crude (SP)-42at is produced as described in Example 267 using (SP)-7at instead of (AP)-7tt. Example 273. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-4-N-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(S_P)-42ct].

[00590] Crude (SP)-42ct is produced as described in Example 267 using (SP)-7ct instead of (RP)-7tt. Example 274. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-2-?/-phenoxyacetyl-deoxyguanosin3'-yl thymidin-5'-yl phosphonate [(S_P)-42gt].

[00591] Crude (SP)-42gt is produced els described in Example 267 using (SP)-7gt instead of (RP)-7tt. Example 275. Synthesis of the alkylhydroxamate pronucleotide of (7?_P)-uridin-3'-yl uridin-5'-yl phosphonate [(R_P)-42uu].

[00592] Crude (7?P)-42uu is produced as described in Example 267 using (RP)-12uu instead of (7?P)-7tt. Example 276. Synthesis of the alkylhydroxamate pronucleotide of (7?_P)-6-7V-Benzoyl-adenosin-3'-yl uridin5'-yl phosphonate [(7?_P)-42au].

[00593] Crude (7?P)-42au is produced as described in Example 267 using (7?P)-12au instead of (7?P)-7tt.

144

Example 277. Synthesis of the alkylhydroxamate pronucleotide of (7?_P)-4-N-Benzoyl-cytidin-3'-yl uridin-5'yl phosphonate [(R_P)-42cu].

[00594] Crude (7?P)-42cu is produced as described in Example 267 using (7?P)-12cu instead of (7?P)-7tt.

Example 278. Synthesis of the alkylhydroxamate pronucleotide of (R_P)-2-V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate [(A_P)-42gu].

[00595] Crude (RP)-42gu is produced as described in Example 267 using (AP)-12gu instead of (7?P)-7tt.

Example 279. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphonate [(S_P)-42uuJ.

[00596] Crude (SP)-42uu is produced as described in Example 267 using (SP)-12uu instead of (7?P)-7tt. Example 280. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-6-jV-Benzoyl-adenosin-3'-yl uridin5'-yl phosphonate [(S_P)-42au].

[00597] Crude (SP)-42au is produced as described in Example 267 using (5P)-12au instead of (2?P)-7tt.

Example 281. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-4-N-Benzoyl-cytidin-3'-yl uridin-5'yl phosphonate [(S_P)-42cu].

[00598] Crude (SP)-42cu is produced as described in Example 267 using (SP)-12au instead of (7?P)-7tt. Example 282. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-2-7V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate [(S_P)-42gu].

[00599] Crude (SP)-42gu is produced as described in Example 267 using (SP)-12gu instead of (7?P)-7tt. Example 283. Synthesis of the alkylhydroxamate pronucleotide of (R_P)-uridin-2'-yl uridin-5'-yl phosphonate [(7?_P)-43uu[.

[00600] Crude (RP)-43uu is produced as described in Example 267 using (/?P)-15uu instead of (7?P)-7tt. Example 284. Synthesis of the alkylhydroxamate pronucleotide of (7?_P)-6-7V-Benzoyl-adenosin-2'-yl uridin5'-yl phosphonate [(/?_P)-43auJ.

[00601] Crude (7?P)-43au is produced as described in Example 267 using (SP)-15au instead of (7?P)-7tt. Example 285. Synthesis of the alkylhydroxamate pronucleotide of (R_P)-4-JV-Benzoyl-cytidin-2'-yl uridin-5'yl phosphonate [(7?_P)-43cuJ.

[00602] Crude (/?P)-43cu is produced as described in Example 267 using (SP)-15cu instead of (RP)-7tt. Example 286. Synthesis of the alkylhydroxamate pronucleotide of (R_P)-2-2V-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl /f-phosphonate [(7?_P)-43gu[.

[00603] Crude (RP)-43gu is produced as described in Example 267 using (SP)-15gu instead of (2?P)-7tt.

Example 287. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphonate [(S_P)-43uu], [00604] Crude (SP)-43uu is produced as described in Example 267 using (5P)-15uu instead of (RP)-7tt.

Example 288. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-6-V-Benzoyl-adenosin-2'-yl uridin5'-yl phosphonate [(S_P)-43auJ.

[00605] Crude (SP)-43au is produced as described in Example 267 using (SP)-15au instead of (7?P)-7tt.

145

Example 289. Synthesis of the alkylhydroxamate pronucleotide of (S_P)-4-JV-Benzoyl-cytidin-2'-yl uridin-5'yl phosphonate [(S_P)-43cuJ.

[00606] Crude (SP)-43cu is produced as described in Example 267 using (SP)-15cu instead of (RP)-7tt.

Example 290. Synthesis of the alkylhydroxamate pronucleotide of (5_p)-2-jV-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphonate [(S_P)-43gu].

[00607] Crude (SP)-43gu is produced as described in Example 267 using (SP)-15gu instead of (5P)-7tt.

Scheme R: Synthesis of acylhydroxamate nucleic acid prodrugs.

<img file="IN720DEN2012A_D0385.tif" />

7tt

<img file="IN720DEN2012A_D0386.tif" />

Example 291. Synthesis of the acylhydroxamate nucleic acid prodrug of (7?_P)-thymidin-3'-yl thymidin-5'-yl phosphonate [(7?_P)-44tt] as described in Scheme R.

[00608] (RP)-5 '-O-(teri-butyldiphenylsilyl)thymidin-3 '-yl 3 '-O-(terNbutyldimethylsilyl)thymidin-5 '-yl 77 phosphonate [(7?P)-7tt] (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). 7V-chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with N acyloxy-N-methyl-3-hydroxypropionamide (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3 x 3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (7?P)-44tt.

Example 292. Synthesis of the acylhydroxamate pronucleotide of (7?_P)-6-7V-benzoyl-deoxyadenosin-3'-yl thymidin-5'-yl phosphonate [(7?_P)-44at].

[00609] Crude (7?P)-40at is produced as described in Example 291 using (7?P)-7at instead of (7?P)-7tt.

Example 293. Synthesis of the acylhydroxamate pronucleotide of (7?_P)-4-7V-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(7?_P)-44ct].

146 [00610]Crude (RP)-44ct is produced as described in Example 291 using (RP)-7ct instead of (RP)-7tt. Example 294. Synthesis of the acylhydroxamate pronucleotide of (R_P)-2-N-phenoxyacetyl-deoxyguanosin3'-yl thymidin-5'-yl phosphonate ](R_P)-44gt].

[00611J Crude (RP)-44gt is produced as described in Example 291 using (RP)-7g instead of (RP)-7tt. Example 295. Synthesis of the acylhydroxamate pronucleotide of (R_P)- thymidin-3'-yl thymidin-5'-yl phosphonate [(R_P)-44ttJ.

[00612] Crude (RP)-44tt is produced as described in Example 291 using (SP)-7tt instead of (RP)-7tt. Example 296. Synthesis of the acylhydroxamate pronucleotide of (S_P)-6-2V-benzoyl-deoxyadenosin-3'-yl thymidin-5’-yl phosphonate [(S_P)-44at].

[00613] Crude (SP)-44at is produced as described in Example 291 using (RP)-7at instead of (RP)-7tt. Example 297. Synthesis of the acylhydroxamate pronucleotide of (S_P)-4-2V-benzoyl-deoxycytidin-3'-yl thymidin-5'-yl phosphonate [(S_P)-44ct].

[00614] Crude (SP)-44ct is produced as described in Example 291 using (RP)-7ct instead of (RP)-7tt.

Example 298. Synthesis of the acylhydroxamate pronucleotide of (R_P)-2-7V-phenoxyacetyl-deoxyguanosin3'-yl thymidin-5'-yl phosphonate [(S_P)-44gt].

[00615] Crude (RP)-44gt is produced as described in Example 291 using (SP)-7gt instead of (RP)-7tt. Example 299. Synthesis of the acylhydroxamate pronucleotide of (R_P)-uridin-3'-yl uridin-5'-yl phosphonate [(R_P)-44uu], [00616] Crude (RP)-44uu is produced as described in Example 291 using (RP)-12uu instead of (RP)-7tt. Example 300. Synthesis of the acylhydroxamate pronucleotide of (R_P)-6-N-Benzoyl-adenosin-3'-yl uridin5'-yl phosphonate [(R_P)-44au].

[00617] Crude (RP)-44au is produced as described in Example 291 using (RP)-12au instead of (RP)-7tt. Example 301. Synthesis of the acylhydroxamate pronucleotide of (R_P)-4-7V-Benzoyl-cytidin-3'-yI uridin-5yl phosphonate [(R_P)-44cu].

[00618] Crude (RP)-44cu is produced as described in Example 291 using (RP)-12cu instead of (RP)-7tt. Example 302. Synthesis of the acylhydroxamate pronucleotide of (R_P)-2-jV-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate ](R_P)-44gu], [00619] Crude (RP)-40gu is produced as described in Example 291 using (RP)-12gu instead of (RP)-7tt. Example 303. Synthesis of the acylhydroxamate pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphonate ](R_P)-44uu].

[00620] Crude (SP)-44uu is produced as described in Example 291 using (SP)-12uu instead of (RP)-7tt.

Example 304. Synthesis of the acylhydroxamate pronucleotide of (R_P)-6-77-Benzoyl-adenosin-3'-yl uridin5'-yl phosphonate [(S_P)-44au].

[00621] Crude (SP)-44au is produced as described in Example 291 using (RP)-12au instead of (RP)-7tt.

Example 305. Synthesis of the acylhydroxamate pronucleotide of (R_P)-4-77-Benzoyl-cytidin-3'-yl uridin-5'yl phosphonate [(S_P)-44cu].

147 [00622]Crude (5P)-44cu is produced as described in Example 291 using (SP)-12au instead of (7?P)-7tt. Example 306. Synthesis of the acylhydroxamate pronucleotide of (S_P)-2-7V-Phenoxyacetyl-guanosin-3'-yl uridin-5'-yl phosphonate [(S_P)-44guJ.

[00623] Crude (SP)-44gu is produced as described in Example 291 using (5P)-12gu instead of (AP)-7tt. Example 307. Synthesis of the acylhydroxamate pronucleotide of (2?_P)-uridin-2'-yl uridin-5'-yl phosphonate [(R_P)-45uu].

[00624J Crude (7?P)-45uu is produced as described in Example 291 using (7?P)-15uu instead of (7?P)-7tt. Example 308. Synthesis of the acylhydroxamate pronucleotide of (2?_P)-6-AT-Benzoyl-adenosin-2'-yl uridin5'-yl phosphonate [(7?_P)-45auJ.

[00625] Crude (5P)-45au is produced as described in Example 291 using (SP)-15au instead of (RP)-7tt. Example 309. Synthesis of the acylhydroxamate pronucleotide of (7?_P)-4-AT-Benzoyl-cytidin-2'-yl uridin-5'yl phosphonate [(R_P)-45cu], [00626] Crude (7?P)-45cu is produced as described in Example 291 using (5P)-15cu instead of (5P)-7tt. Example 310. Synthesis of the acylhydroxamate pronucleotide of (2?_P)-2-AT-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl //-phosphonate [(2?_P)-45guJ.

[00627] Crude (7?P)-45gu is produced as described in Example 291 using (5P)-15gu instead of (7?P)-7tt. Example 311. Synthesis of the acylhydroxamate pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphonate [(S_P)-45uu].

[00628] Crude (5P)-45uu is produced as described in Example 291 using (5P)-15uu instead of (J?P)-7tt. Example 312. Synthesis of the acylhydroxamate pronucleotide of (S_P)-6-N-Benzoyl-adenosin-2'-yl uridin5'-yl phosphonate [(5_P)-45au], [00629] Crude (5P)-45au is produced as described in Example 291 using (SP)-15au instead of (7?P)-7tt. Example 313. Synthesis of the acylhydroxamate pronucleotide of (S_P)-4-jV-Benzoyl-cytidin-2'-yl uridin-5'yl phosphonate [(5_P)-45cu].

[00630]Crude (SP)-45cu is produced as described in Example 291 using (SP)-15cu instead of (RP)-7tt. Example 314. Synthesis of the acylhydroxamate pronucleotide of (5_P)-2-7V-Phenoxyacetyl-guanosin-2'-yl uridin-5'-yl phosphonate [(S_P)-45guJ.

[00631]Crude (SP)-45gu is produced as described in Example 291 using (5P)-I5gu instead of (AP)-7tt.

Scheme S: Synthesis of thiotrialkylammoniumethyl pronucleotides.

148

<img file="IN720DEN2012A_D0387.tif" />

Example 315. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-thymidin-3'-yl thymidin5'-yl phosphorothioate [(R_P)-46tt] as described in Scheme S.

[00632](SP)-l,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(teri-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tert butyldimethylsilyl)thymidin-5'-yl phosphorothioate [(SP)-4tt[ (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry methylene chloride (1 mL). The mixture is treated with vinyltrimethylammonium chloride (100 pmol) in dry (100 pmol) methylene chloride. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stined for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-46tt.

Example 316. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-6-JV-benzoyldeoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-46at].

[00633] Crude (RP)-46at is produced as described in Example 315 using (RP)-4at instead of (RP)-4tt. Example 317. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-4-AT-benzoyldeoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-46ct].

[00634] Crude (RP)-46ct is produced as described in Example 315 using (RP)-4ct instead of (RP)-4tt.

Example 318. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-2-jV-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-46gt].

[00635] Crude (RP)-46gt is produced as described in Example 315 using (RP)-4g instead of (RP)-4tt.

Example 319. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)- thymidin-3'-yl thymidin5'-y 1 phosphorothioate [(5_P)-46tt].

[00636] Crude (SP)-46tt is produced as described in Example 315 using (5P)-4tt instead of (RP)-4tt.

149

Example 320. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-6-?/-benzoyldeoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-46at], [00637] Crude (SP)-46at is produced as described in Example 315 using (SP)-4at instead of (RP)-4tt.

Example 321. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-4-jV-benzoyl-deoxycytidin3 '-yl thymidin-5'-yl phosphorothioate [(S_P)-46ct].

[00638] Crude (SP)-46ct is produced as described in Example 315 using (SP)-4ct instead of (RP)-4tt. Example 322. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (5'_P)-2-A^r-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-46gt].

[00639] Crude (SP)-46gt is produced as described in Example 315 using (SP)-4gt instead of (RP)-4tt. Example 323. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-46uu].

[00640] Crude (RP)-46uu is produced as described in Example 315 using (RP)-10 uu instead of (RP)-4tt. Example 324. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-6-2V-Benzoyl-adenosin-3'yl uridin-5'-yl phosphorothioate [(R_P)-46au[.

[00641] Crude (RP)-46au is produced as described in Example 315 using (RP)-lOau instead of (RP)-4tt. Example 325. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-4-A^r-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-46cu).

[00642] Crude (RP)-46cu is produced as described in Example 315 using (RP)-lOcu instead of (RP)-4tt. Example 326. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-2-jV-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-46gu].

[00643] Crude (RP)-46gu is produced as described in Example 315 using (RP)-lOgu instead of (RP)-4tt. Example 327. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-46uu].

[00644] Crude (SP)-46uu is produced as described in Example 315 using (SP)-lOuu instead of (RP)-4tt. Example 328. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-6-jV-Benzoyl-adenosin-3'yl uridin-5'-yl phosphorothioate [(5_P)-46au].

[00645] Crude (SP)-46au is produced as described in Example 315 using (SP)-lOau instead of (RP)-4tt. Example 329. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-4-AT-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-46cuJ.

[00646]Crude (SP)-46cu is produced as described in Example 315 using (SP)-lOau instead of (RP)-4tt.

Example 330. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-2-lV-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-46gu].

[00647] Crude (SP)-46gu is produced as described in Example 315 using (SP)-lOgu instead of (RP)-4tt.

Example 331. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(R_P)-47uu].

[00648] Crude (SP)-47uu is produced as described in Example 315 using (SP)-14uu instead of (RP)-4tt.

150

Example 332. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (2?_P)-6-N-Benzoyl-adenosin-2'yl uridin-5'-yl phosphorothioate [(7?_P)-47au].

[00649] Crude (RP)-47au is produced as described in Example 315 using (SP)-14au instead of (J?P)-4tt.

Example 333. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-4-W-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(/?_P)-47cu].

[00650] Crude (7?P)-47cu is produced as described in Example 315 using (SP)-14cu instead of (J?P)-4tt. Example 334. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-2-N-Phenoxyacetylguanosin-2'-yl uridin-5'-yl phosphorothioate [(R_P)-47gu], [00651] Crude (RP)-47gu is produced as described in Example 315 using (SP)-14gu instead of (RP)-4tt. Example 335. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-uridin-2'-yI uridin-5'-yl phosphorothioate [(S_P)-47uu].

[00652] Crude (SP)-47uu is produced as described in Example 315 using (SP)-14uu instead of (AP)-4tt.

Example 336. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-6-Y-Benzoyl-adenosin-2'yl uridin-5'-yl phosphorothioate [(S_P)-47au).

[00653] Crude (SP)-47au is produced as described in Example 315 using (SP)-14au instead of (RP)-4tt. Example 337. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-4-N-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(^)-47^].

[00654] Crude (SP)-47cu is produced as described in Example 315 using (SP)-14cu instead of (7?P)-4tt. Example 338. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (S_P)-2-N-Phenoxyacetylguanosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-47gu].

[00655] Crude (SP)-47gu is produced as described in Example 315 using (SP)-14gu instead of (7?P)-4tt.

Scheme T: Synthesis of thio N-alkylhydroxamate pronucleotides.

<img file="IN720DEN2012A_D0388.tif" />

«t

<img file="IN720DEN2012A_D0389.tif" />

Example 339. Synthesis of the thio N-alkylhydroxamate pronucleotide of (7?_P)-thymidin-3'-yl thymidin-5'yl phosphorothioate [(R_P)-48tt] as described in Scheme T.

[00656](SP)-l ,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(teri-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tert butyldimethylsilyl)thymidin-5'-yl phosphorothioate [(SP)-4tt] (100 pmol) is dried by repeated 151 coevaporations with dry pyridine and then dissolved in dry methylene chloride (1 mL). The mixture is treated with N,O-dimethylacrylamide (100 pmol) in dry (100 μτηοΐ) methylene chloride. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-48tt.

Example 340. Synthesis of the thio N-alkylhydroxamate pronucleotide of (5_P)-6-jV-benzoyl-deoxyadenosin3 '-yl thymidin-5'-yl phosphorothioate [(7?_P)-48atJ.

[00657] Crude (7?P)-48at is produced as described in Example 339 using (RP)-4at instead of (5P)-4tt. Example 341. Synthesis of the thio N-alkylhydroxamate pronucleotide of (R_P)-4-JV-benzoyl-deoxycytidin3 '-yl thymidin-5'-yl phosphorothioate [(7?_P)-48ctJ.

[00658] Crude (RP)-48ct is produced as described in Example 339 using (RP)-4ct instead of (RP)-4tt. Example 342. Synthesis of the thio N-alkylhydroxamate pronucleotide of (2?_P)-2-N-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-48gt).

[00659] Crude (RP)-48gt is produced as described in Example 339 using (RP)-4g instead of (RP)-4tt. Example 343. Synthesis of the thio N-alkylhydroxamate pronucleotide of (5_P)- thymidin-3'-yl thymidin-5'yl phosphorothioate [(S_P)-48tt].

(00660] Crude (5P)-48tt is produced as described in Example 339 using (5P)-4tt instead of (RP)-4tt. Example 344. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-6-7V-benzoyl-deoxyadenosin3 '-yl thymidin-5'-yl phosphorothioate [(S_P)-48at(.

[00661] Crude (5P)-48at is produced as described in Example 339 using (5P)-4at instead of (AP)-4tt. Example 345. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-4-AT-benzoyl-deoxycytidin3'-yl thymidin-5'-yl phosphorothioate [(5_P)-48ct], [00662] Crude (SP)-48ct is produced as described in Example 339 using (SP)-4ct instead of (5P)-4tt. Example 346. Synthesis of the thio N-alkylhydroxamate pronucleotide of (5_P)-2-N'-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(5_P)-48gt(.

[00663] Crude (5P)-48gt is produced as described in Example 339 using (5P)-4gt instead of (7?P)-4tt.

Example 347. Synthesis of the thio N-alkylhydroxamate pronucleotide of (/?_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-48uu(.

[00664] Crude (RP)-48uu is produced as described in Example 339 using (RP)-lOuu instead of (7?P)-4tt.

Example 348. Synthesis of the thio N-alkylhydroxamate pronucleotide of (R_P)-6-N-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-48au].

[00665] Crude (RP)-48au is produced as described in Example 339 using (RP)-lOau instead of (7?P)-4tt.

152

Example 349. Synthesis of the thio N-alkylhydroxamate pronucleotide of (A_P)-4-7V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(A_P)-48cuJ, [00666] Crude (AP)-48cu is produced as described in Example 339 using (AP)-lOcu instead of (AP)-4tt.

Example 350. Synthesis of the thio N-alkylhydroxamate pronucleotide of (R_P)-24V-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphorothioate [(A_P)-48gu], [00667] Crude (AP)-48gu is produced as described in Example 339 using (AP)-lOgu instead of (AP)-4tt. Example 351. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(5_P)-48uu].

[00668] Crude (5P)-48uu is produced as described in Example 339 using (AP)-lOuu instead of (AP)-4tt. Example 352. Synthesis of the thio N-alkylhydroxamate pronucleotide of (5_P)-6-iV-Benzoyl-adenosin-3'-yl uridin-5'-yl phosphorothioate [(5_P)-48au].

[00669] Crude (SP)-48au is produced as described in Example 339 using (SP)-lOau instead of (AP)-4tt. Example 353. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-4-jV-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-48cu].

[00670] Crude (5P)-48cu is produced as described in Example 339 using (SP)-lOau instead of (AP)-4tt. Example 354. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-24V-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-48guJ.

[00671] Crude (SP)-48gu is produced as described in Example 339 using (5P)-10gu instead of (AP)-4tt. Example 355. Synthesis of the thio N-alkylhydroxamate pronucleotide of (A_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(A_P)-49uu].

[00672] Crude (SP)-49uu is produced as described in Example 339 using (SP)-14uu instead of (AP)-4tt. Example 356. Synthesis of the thio N-alkylhydroxamate pronucleotide of (R_P)-64V-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(A_P)-49au].

[00673] Crude (AP)-49au is produced as described in Example 339 using (SP)-14au instead of (AP)-4tt. Example 357. Synthesis of the thio N-alkylhydroxamate pronucleotide of (A_P)-4-N-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(A_P)-49cuJ.

[00674] Crude (AP)-49cu is produced as described in Example 339 using (SP)-14cu instead of (AP)-4tt. Example 358. Synthesis of the thio N-alkylhydroxamate pronucleotide of (A_P)-2-jV-Phenoxyacetylguanosin-2'-yl uridin-5’-yl phosphorothioate [(A_P)-49gu].

[00675] Crude (RP)-49gu is produced as described in Example 339 using (SP)-14gu instead of (AP)-4tt.

Example 359. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-49uu].

[00676] Crude (SP)-49uu is produced as described in Example 339 using (SP)-14uu instead of (AP)-4tt.

Example 360. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-64V-Benzoyl-adenosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-49auJ.

[00677] Crude (SP)-49au is produced as described in Example 339 using (SP)-14au instead of (AP)-4tt.

153

Example 361. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-4-N-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-49cuJ.

[00678]Crude (SP)-49cu is produced as described in Example 339 using (SP)-14cu instead of (RP)-4tt. Example 362. Synthesis of the thio N-alkylhydroxamate pronucleotide of (S_P)-2-jV-Phenoxyacetylguanosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-49gu].

[00679J Crude (5P)-49gu is produced as described in Example 339 using (SP)-14gu instead of (7?P)-4tt.

Scheme U: Synthesis of thio N-acetoxyhydroxamate pronucleotides.

OTBS

OTBS

4tt

Example 363. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (R_P)-thymidin-3'-yl thymidin5'-yl phosphorothioate [(R_P)-50tt] as described in Scheme U.

[00680](SP)-l ,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(tert-butyldiphenylsilyl)thymidin-3'-yl 3'-O-(tertbutyldimethylsilyl)thymidin-5'-yl phosphorothioate [(SP)-4tt] (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry methylene chloride (1 mL). The mixture is treated with N-methyl-N-acetoxy-acrylamide (100 pmol) in dry (100 pmol) methylene chloride. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3 x 3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (7?P)-50tt.

Example 364. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (R_P)-6-N-benzoyldeoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-50at].

[00681] Crude (7?P)-50at is produced as described in Example 363 using (7?P)-4at instead of (7?P)-4tt. Example 365. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (R_P)-4-N-benzoyldeoxycytidin-3'-yl thymidin-5’-yl phosphorothioate [(2?_P)-50ct].

[00682] Crude (RP)-50ct is produced as described in Example 363 using (RP)-4ct instead of (J?P)-4tt.

154

Example 366. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (7?_P)-2-jV-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-50gt).

[00683] Crude (AP)-50gt is produced as described in Example 363 using (AP)-4g instead of (7?P)-4tt.

Example 367. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)- thymidin-3'-yl thymidin5'-yl phosphorothioate [(S_P)-50tt].

[00684] Crude (SP)-50tt is produced as described in Example 363 using (SP)-4tt instead of (RP)-4tt.

Example 368. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-6-JV-benzoyldeoxyadenosin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-50at[.

[00685] Crude (SP)-50at is produced as described in Example 363 using (SP)-4at instead of (7?P)-4tt. Example 369. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (5_P)-4-jV-benzoyldeoxycytidin-3'-yl thymidin-5'-yl phosphorothioate [(S_P)-50ct[.

[00686] Crude (SP)-50ct is produced as described in Example 363 using (SP)-4ct instead of (7?P)-4tt. Example 370. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S^,P)-2-A^r-phenoxyacetyldeoxyguanosin-3'-yl thymidin-5'-yl phosphorothioate [(SP)-50gt[.

[00687] Crude (SP)-50gt is produced as described in Example 363 using (SP)-4gt instead of (RP)-4tt. Example 371. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (7?_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-50uu], [00688]Crude (2?P)-50uu is produced as described in Example 363 using (RP)-lOuu instead of (RP)-4tt. Example 372. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (7?_P)-6-jV-Benzoyl-adenosin-3'yl uridin-5'-yl phosphorothioate [(7?_P)-50au], [00689] Crude (RP)-50au is produced as described in Example 363 using (RP)-lOau instead of (RP)-4tt. Example 373. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (l?_P)-4-2V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(R_P)-50cu].

[00690] Crude (7?P)-50cu is produced as described in Example 363 using (RP)-lOcu instead of (7?P)-4tt. Example 374. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (7?_P)-2-7V-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphorothioate [(7?_P)-50guJ.

[00691] Crude (RP)-50gu is produced as described in Example 363 using (AP)-lOgu instead of (RP)-4tt. Example 375. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-uridin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-50uu].

[00692] Crude (SP)-50uu is produced as described in Example 363 using (SP)-lOuu instead of (RP)-4tt.

Example 376. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-6-/V-Benzoyl-adenosin-3'yl uridin-5'-yl phosphorothioate [(S_P)-50auJ.

[00693] Crude (SP)-50au is produced as described in Example 363 using (SP)-lOau instead of (7?P)-4tt.

Example 377. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-4-/V-Benzoyl-cytidin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-50cu[.

[00694] Crude (SP)-50cu is produced as described in Example 363 using (SP)-lOau instead of (7?P)-4tt.

155

Example 378. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (5_P)-2-7V-Phenoxyacetylguanosin-3'-yl uridin-5'-yl phosphorothioate [(S_P)-50gu], [00695] Crude (SP)-50gu is produced as described in Example 363 using (SP)-lOgu instead of (I?P)-4tt.

Example 379. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (7?_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(7?_P)-51 uu].

[00696] Crude (SP)-51uu is produced as described in Example 363 using (SP)-14uu instead of (AP)-4tt. Example 380. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (7?_P)-6-jV-Benzoyl-adenosin-2'yl uridin-5'-yl phosphorothioate [(7?_P)-51auJ.

[00697] Crude (7?P)-51au is produced as described in Example 363 using (SP)-14au instead of (AP)-4tt. Example 381. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (R_P)-4-Ar-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(R_P)-51cu].

[00698] Crude (7?P)-51cu is produced as described in Example 363 using (SP)-14cu instead of (7?P)-4tt. Example 382. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (/?_P)-2-N-Phenoxyacetylguanosin-2'-yl uridin-5'-yl phosphorothioate [(J?_P)-51 gu].

[00699] Crude (7?P)-51gu is produced as described in Example 363 using (SP)-14gu instead of (7?P)-4tt. Example 383. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-uridin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-51uu].

[00700] Crude (SP)-51uu is produced as described in Example 363 using (SP)-14uu instead of (2?P)-4tt. Example 384. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-6-7V-BenzoyI-adenosin-2'yl uridin-5'-yl phosphorothioate [(S_P)-51au[.

[00701]Crude (SP)-51au is produced as described in Example 363 using (SP)-14au instead of (7?P)-4tt. Example 385. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-4-?/-Benzoyl-cytidin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-51cuJ.

(00702] Crude (SP)-51cu is produced as described in Example 363 using (SP)-14cu instead of (7?P)-4tt. Example 386. Synthesis of the thio N-acetoxyhydroxamate pronucleotide of (S_P)-2-jV-Phenoxyacetylguanosin-2'-yl uridin-5'-yl phosphorothioate [(S_P)-51gu].

(00703] Crude (SP)-51gu is produced as described in Example 363 using (SP)-14gu instead of (J?P)-4tt.

Scheme V: Synthesis of thiotrialkylammoniumethyl pronucleotides.

156

<img file="IN720DEN2012A_D0390.tif" />

<img file="IN720DEN2012A_D0391.tif" />

Example 387. Synthesis of the thiotrialkylammoniumethyl pronucleotide of (R_P)-thymidin-3'-yl thymidin5'-yl phosphorothioate [(R_P)-53tt] as described in Scheme V.

[00704] (SP)-l ,8-Diazabicyclo[5.4.0]undec-7-enium 5'-O-(dimethoxytrity)thymidin-3'-yl 3'-O(dimethoxytrity)thymidin-5'-yl phosphorothioate [(SP)-52tt] is prepared by the same method used for the preparation of compound 4tt in Example 1 (Scheme A). Compound (SP)-52tt (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry dimethylformamide (1 mL). The mixture is treated with 2-iodoethyl trimethylammonium iodide (100 pmol) in dry DMF (0.5 mL). After 1 hour, the mixture is concentrated and then dissolved in CH2C12 (1000 pL) and trichloroacetic acid (50 pmol) is added. The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-53tt.

Scheme W: Synthesis of thiotrialkylammoniumethyl pronucleotides.

157

<img file="IN720DEN2012A_D0392.tif" />

o₂n

CI3CCO2H

<img file="IN720DEN2012A_D0393.tif" />

Example 388. Synthesis of the disulfide pronucleotide of (l?_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(7?_P)-54tt] as described in Scheme W.

[00705] Compound (SP)-52tt (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry ethanol (1 mL). The mixture is treated with p-nitrobenezene sulfenyl chloride (200 pmol) in dry (100 pmol) ethanol. After 1 hour, the mixture is concentrated and then dissolved in CH2C12 (1000 pL) and trichloroacetic acid (50 pmol) is added. The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (7?P)-54tt.

Scheme X: Synthesis of 2-thiopivalylethyl nucleic acid prodrugs.

<img file="IN720DEN2012A_D0394.tif" />

ODMTr ODMTr

55tt

ClaCCCfcH

<img file="IN720DEN2012A_D0395.tif" />

Example 389. Synthesis of the 2-thiopivalylethyl nucleic acid prodrug of (7?_P)-thymidin-3'-yl thymidin-5'yl phosphonate [(R_P)-56tt] as described in Scheme X.

158 [00706] (RP)-5'-O-(dimethoxytrityl)thymidin-3'-yl 3'-O-(dimethoxytrityl)thymidin-5'-yl //-phosphonate [(RP)-55tt[ is prepared by the same method used for the preparation of compound 7tt in Example 8 (Scheme B). Compound (RP)-55tt (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). N-Chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with 2-hydroxyethylthiopivalate (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in CH2C12 (1000 pL) and trichloroacetic acid (50 pmol) is added. The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3x3 mL). The combined organic layers are backextracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-56tt.

Scheme Y:

Synthesis of 2-carboethoxyethyl nucleic acid prodrugs.

<img file="IN720DEN2012A_D0396.tif" />

ch₃cn pyridine

ODMTr

55tt

ODMTr

CI3CCO2H

<img file="IN720DEN2012A_D0397.tif" />

Example 390. Synthesis of the 2-carboethoxyethyl nucleic acid prodrug of (R_P)-thymidin-3'-yl thymidin-5'yl phosphonate [(R_P)-57tt] as described in Scheme Y.

[00707] Compound (RP)-55tt (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). N-Chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with ethyl 2-hydroxyethylpropionate (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in CH2C12 (1000 pL) and trichloroacetic acid (50 pmol) is added. The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column

159 chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-57tt.

Scheme Z: Synthesis of thio(cyclohexyl)acyloxy pronucleotides.

<img file="IN720DEN2012A_D0398.tif" />

ODMTr

52tt

<img file="IN720DEN2012A_D0399.tif" />

Example 391. Synthesis of the thio(cyclohexyl)acyloxy pronucleotide of (7?_P)-thymidin-3'-yl thymidin-5'-yl phosphorothioate [(R_P)-58tt] as described in Scheme Z.

[00708] Compound (SP)-52tt (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry methylene chloride (1 mL). The mixture is treated with chloromethylcyclohexylacetic acetate (100 pmol) in dry (100 pmol) methylene chloride. After 1 hour, the mixture is concentrated and then dissolved in CH2C12 (1000 pL) and trichloroacetic acid (50 pmol) is added. The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-58tt.

Scheme AA: Synthesis of 2-carboxyethyl nucleic acid prodrugs.

<img file="IN720DEN2012A_D0400.tif" />

160

Example 392. Synthesis of the 2-carboxyethyl nucleic acid prodrug of (R_P)-thymidin-3'-yl thymidin-5'-yl phosphonate [(R_P)-59tt] as described in Scheme AA.

[00709] Compound (RP)-55tt (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). N-Chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with tert-butyl 2-hydroxyethylpropionate (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in CH2C12 (1000 pL) and trichloroacetic acid (50 pmol) is added. The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-59tt.

Scheme BB: Synthesis of 2-((2-hydroxyethyl)disuIfide)ethyl nucleic acid prodrugs.

TBDPSOq^o^a &gt;° ⁰

OTBS

<img file="IN720DEN2012A_D0401.tif" />

CH3CN pyridine

<img file="IN720DEN2012A_D0402.tif" />

<img file="IN720DEN2012A_D0403.tif" />

7tt

Et3N3HF

<img file="IN720DEN2012A_D0404.tif" />

Example 393. Synthesis of the 2-((2-hydroxyethyi)disulfide)ethyi nucleic acid prodrug of (R_P)-thymidin-3'yl thymidin-5'-yl phosphonate [(R_P)-60tt] as described in Scheme BB.

[00710] Compound (RP)-7tt (100 pmol) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). H-Chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with 2-((2-(tert-butyldiphenylsilyloxy)ethyl)disulfanyl)ethanol (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in triethylamine trihydrofluoride (500 pL). The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et20 (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column

161 chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (7?P)-60tt.

Scheme CC: Synthesis of 2-(methanesulfonothioate)ethyl nucleic acid prodrugs.

<img file="IN720DEN2012A_D0405.tif" />

ODMTr

55tt

<img file="IN720DEN2012A_D0406.tif" />

CH₃CN pyridine

<img file="IN720DEN2012A_D0407.tif" />

ODMTr

CbCCQjH

<img file="IN720DEN2012A_D0408.tif" />

Example 394. Synthesis of the 2-(methanesulfonothioate)ethyl nucleic acid prodrug of (R_P)-thymidin-3'-yl thymidin-5'-yl phosphonate [(R_P)-61 tt] as described in Scheme CC.

[00711] Compound (AP)-55tt (100 μιηοΐ) is dried by repeated coevaporations with dry pyridine and then dissolved in dry pyridine (1 mL). N-Chlorosuccinimide (0.1 mmol) is added, and the mixture is stirred for 2 hours at 0 °C. The mixture is concentrated and dissolved in dry pyridine (1 mL). The above mixture is treated with S-2-hydroxyethyl methanesulfonothioate (100 pmol) in dry (100 pmol) pyridine. After 1 hour, the mixture is concentrated and then dissolved in CH2C12 (1000 pL) and trichloroacetic acid (50 pmol) is added. The mixture is stirred for 15 h at room temperature. A 0.1 M ammonium acetate buffer (2.5 mL) is then added to the mixture, and the mixture is washed with Et2O (3x3 mL). The combined organic layers are back-extracted with 0.1 M ammonium acetate buffer (3 mL). The combined aqueous layers are then concentrated to dryness under reduced pressure, and the residue is purified by reverse-phase column chromatography [a linear gradient of acetonitrile 0-10% in 0.1 M ammonium acetate buffer (pH 7.0)] to afford (RP)-61tt.

Scheme DD: Synthesis of Prodrug molecules from H-Phosphonate Thymidine dimer [00712] Chemoselectivity and stereospecificity of iodine mediated oxidative couplings using separate diastereomers of dinucleoside H-phosphonate and O- nucleophiles to prepare phosphotriesters are known in the art (Nucleosides, Nucleotides &amp; Nucleic Acids 2003 Vol. 22, Nos. 5-8, 1467-1469). The products were purified by silica gel chromatography and characterized by 3IP and 1H NMR spectroscopy. The products were further purified by reverse phase HPLC for kinetic studies.

162

Scheme DD

<img file="IN720DEN2012A_D0409.tif" />

63a R=SCH₂CH₂OTBDMS ^64a R=SCH₂CH₂OH

63b R=SO₂CH₃^64b R=SO₂CH₃

63c R=SCO(CH₃)₃^64c R=SCO(CH₃)₃

i) I₂, ACN: Py (3:2), TBDPSC1 and 2-((2-((ier/-butyldimethylsilyl)oxy)ethyl)disulfanyl)ethanol for 63a; 2hydroxyethyl mehanesulfonate for 63b and 63c) 2-Hydroxyethylthiopivalate ii) 3% DCA/DCM

Example 395. General Procedure for Synthesis of 63a, 63b and 63c (Scheme DD) [00713] (RP,SP)-5'-O-(4,4’-dimethoxytrityl)thymidin-3'-yl 3'-O-(4,4’-dimethoxytrityl)thymidin-5'-yl Hphosphonate (62) (113.5 mg, 100 pmol) was dried under high vacuum overnight and dissolved in ACN (2 ml) and pyridine (2 ml). tert-Butyldiphenylsilyl chloride (52 pL, 200 pmol) and 12 (76 mg, 300 pmoles) were added. The reaction mixture was cooled in ice and respective alkylating reagent (1 mmol) dissolved in ACN (2 mL) was added drop wise to reaction mixture. The mixture was stirred for 10 min under argon. TLC of the crude reaction mixture showed quantitative conversion to product. The solvents were evaporated and residue was dissolved in ethyl acetate and washed with 5% Na2S2O3, brine and dried over Na2SO4. The ethyl acetate layer was concentrated under reduced pressure. The residue was purified by silica gel column chromatography to afford (RP,SP)-5'-O-(4,4’-dimethoxytrityl)thymidin-3'-yl 3'-O-(4,4’dimethoxytrityl)thymidin-5'-yl phosphotriester 63a, 63b and 63c in 80-90% yield.

General Procedure for Synthesis of 64a, 64b and 64c [00714] 3% DCA/DCM was added slowly to DMTr protected triester and reaction was left for stirring at room temperature for 30min. Reaction was quenched with methanol, solvents were evaporated and residue was purified by silica column. In case of compound 63a, TBDMS deprotection occurred simultaneously. Compounds 64a, 64b and 64c were obtained in quantitative yields.

[00715] Compound 63a: 1H NMR (400 MHz, CDC13) &lt;5 7.58-7.18 (m, 20H), 6.87-6.78 (m, 8H), 6.49-6.27 (m, 2H), 5.11-5.07 (m, 1H), 4.2-4.05 (m, 3H), 3.99-3.87 (m, 1H), 3.85-3.73 (m, 13H), 3.73-3.56 (m, 1H),

163

3.54- 3.26 (m, 2H), 2.94-2.66 (m, 8H), 1.97-1.78(m, 4H), 1.76-1.54 (m, 1H), 1.43-1.3 (m, 3H), 0.94-0.78 (m, 9H), 0.11-0.03 (m, 6H). ³¹P NMR (162 MHz, CDC13) &lt;5-1.19,-1.26 (two diastereomers).

[00716] Compound 63b: 1H NMR (400 MHz, CDC13 + trace amount of Py-D5) &lt;5 9.72-9.45 (m, 2H), 7.627.18 (m, 20H), 6.90-6.81 (m, 8H), 6.48-6.31 (m, 2H), 5.18-5.10 (m, 1H), 4.31—4.09 (m, 3H), 4.03-3.91 (m, 1H), 3.88-3.74 (m, 15H), 3.74-3.62 (m, 1H), 3.54-3.31 (m, 2H), 2.89-2.76 (m, 4H), 2.67-2.31 (m, 2H), 1.98-1.89 (m, 1H), 1.88, 1.85 (2s, 3H, diastereomers), 1.76-1.64 (m, 1H), 1.39 (s, 3H). ³¹P NMR (162 MHz, CDC13) &lt;5 -1.24, -1.27 (two diastereomers).

[00717] Compound 63c: 1H NMR (400 MHz, CDC13 + trace amount of Py-D5) &lt;5 7.6-7.16 (m, 20H), 6.96.77 (m, 8H), 6.49-6.27 (m, 2H), 5.18-5.06 (m, 1H), 4.32-4.04 (m, 2H), 4.0-3.85 (m 3H), 3.82-3.71 (m, 12H), 3.71-3.57 (m, 1H), 3.55-3.27 (m, 2H), 3.07-2.88 (m, 2H), 2.62-2.24 (m, 2H), 1.97-1.89 (m, 1H), 1.891.81 (m, 3H), 1.78-1.59 (m, 3H), 1.45-1.32 (m, 3H), 1.22-1.14 (m, 9H). ³¹P NMR (162 MHz, CDC13) &lt;5 1.23, -1.27 (two diastereomers).

[00718]Compound 64a: 1H NMR (400 MHz, D2O) &lt;5 7.52 (s, 1H), 7.42 (s, 1H), 6.28-6.07 (m, 2H), 5.074.87 (m, 1H), 4.54-4.38 (m, 1H), 4.37-4.19 (m, 3H), 4.19-3.95 (m, 2H), 3.80-3.50 (m, 4H), 2.99-2.62 (2m, 4H), 2.60-2.17 (m, 4H), 1.85-1.60 (m, 6H). ³¹P NMR (162 MHz, CD3OD) δ -1.37, -1.41 (two diastereomers). Calculated mass = 682.66, Observed mass in ESI-ve mode = 681.22 [00719]Compound 64b: 1H NMR (400 MHz, CD3OD) δ 7.74,7.49 (2s, 2H), 6.28-6.19 (m, 2H), 5.10-5.04 (m, 1H), 4.41-4.23 (m, 4H), 4.19^1.15 (m, 1H), 4.04-3.98 (m, 1H), 3.78-3.69 (m, 4H), 3.00-2.77 (m, 4H),

2.55- 2.21 (m, 4H), 1.86, 1.83 (2s, 6H). ³¹P NMR (162 MHz, CD3OD) &lt;5 -1.37, -1.41 (two diastereomers). Calculated mass = 684.63, Observed mass in ESI+ve mode = 683.14 [00720] Compound 64c: 1H NMR (400 MHz, CD3OD) δ 7.799 (s, 1H), 7.54 (s, 1H), 6.32-6.24 (m, 2H), 5.17-5.07 (m, 1H), 4.48-4.26 (m, 3H), 4.26-4.12 (m, 3H), 4.08-3.99 (m, 1H), 3.21-3.15 (m, 2H), 2.61-2.48 (m, 1H), 2.46-2.16 (m, 3H), 1.9 (s, 3H), 1.87 (s, 3H), 1.27-1.19 (d, 9H). ³¹P NMR (162 MHz, CD3OD) δ 1.53,-1.60 (two diastereomers). Calculated mass = 690.66, Observed mass in ESI-ve mode = 689.53

HPLC purification of 64a, 64b and 64c [00721] Reverse phase purification was carried out using Waters 2525 BGM combined with 2487 UV detector, Phenomenex Luna 5u C18 (2) 100A, 250x10 mm column and MassLynx v4.1. A gradient of Water and acetonitrile was used with flow rate of 5ml/min.

[00722] Gradient used for compound 64a and 64b: 10 to 50% B in 30 min [00723] Gradient used for compound 64c: 20 to 60% B in 30 min [00724] The product peaks were monitored at 254 and 280 nm.

Analytical HPLC conditions [00725] Quantitative analysis was done by reverse-phase HPLC employing an automated Alliance Waters e2695 HPLC instrument in combination with Empower software. An XBridge C18 3.5um, 4.6x150mm, Waters part# 186003034A was fitted and detection was done by UV (254nm and 280nm). A gradient elution 164 system was developed (Table 1) enabling the resolution of prodrug, intermediate and the released drug within the same chromatogram; mobile phase A consisting of 20 mM ammonium acetate in water; mobile phase B was acetonitrile.

Table 1

Column temperature: 60°C

<td> Time</td><td> Flow</td><td> %A</td><td> %B</td><td> Curve</td>

<td> 0.01</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td></td>

<td> 5.00</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td> 1</td>

<td> 30.00</td><td> 1.00</td><td> 75.0</td><td> 25.0</td><td> 6</td>

<td> 30.50</td><td> 1.00</td><td> 10.0</td><td> 90.0</td><td> 6</td>

<td> 35.00</td><td> 1.00</td><td> 10.0</td><td> 90.0</td><td> 1</td>

<td> 35.50</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td> 6</td>

<td> 42.00</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td> 1</td>

Example 396. Glutathione assisted prodrug release [00726] To 20 pL of 64 in water (2 O.D.), 100 pL of 10X PBS, and 630 pL of H2O were mixed. Kept the mixture in a hot plate set at 37 °C. 250 pL of freshly prepared 20 mM reduced L-glutathione was added to above mixture which gave 5 mM GSH concentration in the reaction mixture which is equal to cytosol concentration. 100 pl aliquots were take at time intervals of 10 min, 20 min, 30 min, 40 min, 50 min, 60 min, 1.5 hr, 2 hr and 2.5 hr. Each aliquot was immediately quenched with 400 pl of 100 mM citrate buffer (pH 4) and analyzed by reversed-phase HPLC and LC/MS.

Scheme EE: Mechanism of Glutathione Cleavage

<img file="IN720DEN2012A_D0410.tif" />

HO HO HO (Sp+ Rp)

64a, R=SCH₂CH₂OH

64b, R=SO₂CH, irteSate trapped at pH 4 Phosphodiester and observed by HPLC

LCMS of reaction mixture of compound 64b + GSH at 20 min time point [00727] Waters Acquity UPLC and SDS were used to characterize the products formed during prodrug release. XBridge cl8 3.5um, 4.6x150mm,Waters part# 186003034 was used with solvent system A: 5 mM ammonium formate/water and B: acetonitrile with linear gradient as shown in Table 2.

Table 2

<td> Time</td><td> Flow</td><td> %A</td><td> %B</td><td> Curve</td>

<td> 0.0</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td></td>

<td> 5.00</td><td> 1.00</td><td> 80</td><td> 20</td><td> 6</td>

165

<td> 7</td><td> 1.00</td><td> 5</td><td> 95</td><td> 6</td>

<td> 7.5</td><td> 1.00</td><td> 99</td><td> 1</td><td> 6</td>

<td> 9</td><td> 1.00</td><td> 99</td><td> 1</td><td> 1</td>

[00728] In Figure 1 is provided a representative HPLC profile of compound 64a + GSH.

(00729] In Figure 2 is provided a representative HPLC profile of compound 64a, a glutathione adduct, and the final product after release from the pro-moeity.

[00730] In Figure 3, compounds 64a and 64b show a pseudo first order kinetics because glutathione concentration is in great excess compared to substrate and thus remains effectively constant during the course of reaction. The curves for depleting starting material and forming product are not minor images because of accumulation of intermediate which is characterized as Glutathione adduct of dinucleoside triester (see Figure 2 and Figure 4).

Example 397. Carboxyesterase assisted cleavage of compound 64c

Scheme FF

<img file="IN720DEN2012A_D0411.tif" />

Compound 64c Phosphodiester [00731]Porcine Liver esterase (Sigma Aldrich, product number: E2884) was a suspension in 3.2M ammonium sulfate pH=8.0, concentration 36 mg protein/mL and 154 units/mg protein. According to product specifications, one unit will hydrolyse 1 μΜ of ethyl butyrate to butyric acid and ethanol per minute at pH=8.0 at 25°C. Compound 64c (0.1O.D, 5.5 nmoles) in 10 pL 1XPBS was incubated at 37 °C for 10 min. Serial dilutions of PLE were made in ten vials with cone in units from 1, 10-1,10-2,10-3,10-4, 10-5, 10-6, 10-7, 10-8, 10-9 each in 10 pL 1XPBS. The protein solution was incubated at 37 °C for 10 min and then each added to ten vials containing compound 64c. The mixtures were stored at 37 °C for 30 min and analysed by analytical HPLC and LCMS. The 64c was completely converted to phosphodiester in vials with protein cone, from 1, 10-1 and 10-2. No side reactions were observed. There was no reaction in vials with protein cone, from 10-6 to 10-9. There was some product seen in the vials containing protein cone. 10-3 and 10-4. This suggests that these concentrations are appropriate to study the kinetics of prodrug release using PLE. Time dependent kinetics will be studied using cone, within the range of 10-3 to 10-4 /~6 nmoles of compound 64c.

166 [00732] Compound 64c (5 O.D., 2.9 pmoles) dissolved in 900 pL of 1XPBS was incubated at 37 °C for 10 min. Porcine Liver Esterase (1U) in 100 pL lxPBS was added to above mixture and was stored at 37 °C. Aliquots of 100 pL were withdrawn at 0 min, 15 min and 45 min, quenched with 100 pL acetonitrile and samples were cooled in ice-bath. The samples were analyzed by UPLC SQD on XBridge C-18 3.5 pm, 4.6 x 150 mm, with solvent system A: 5 mM ammonium formate/water and B: acetonitrile with linear gradient as shown in Table 3. At zero minutes, only compound 64c was observed, at 15 min nearly 50% of the product was formed and reaction was complete at 45 minutes. Thus TpT diester 64c was released by carboxyesterase treatment without detectable accumulation of any intermediates.

Table 3

<td> Time</td><td> Flow</td><td> %A</td><td> %B</td><td> Curve</td>

<td> 0.0</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td></td>

<td> 2.0</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td> 1</td>

<td> 7.0</td><td> 1.00</td><td> 60.0</td><td> 40.0</td><td> 6</td>

<td> 9.0</td><td> 1.00</td><td> 5.0</td><td> 95.00</td><td> 6</td>

<td> 9.5</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td> 6</td>

<td> 11.0</td><td> 1.00</td><td> 99.0</td><td> 1.0</td><td> 1</td>

Example 398. Treatment of Pancreatic Cancer [00733] A method for treating a subject having pancreatic cancer comprising administering to the subject a therapeutically effective amount of a composition comprising the 2’-5’-A3 S-acetyl-2-thioethyl pronucleotide of Example 242 is contemplated. Treatment is expected to achieve increased tumor inhibition compared with gemcitabine administered as a single agent or gemcitabine and erlotinib administered in combination.

Example 399. Cell Penetration Assay

P³²'Labeled Nucleic Acid Drugs [00734] Prepare labeled nucleic acid prodrug and parent drug using [³²P]dNTP radionucleotide (Fisher Scientific, Pittsburgh, PA) to synthesize nucleic acid molecules comprising chiral phosphorous moieties and corresponding parent drugs as described herein.

Cell Culture and Penetration Testing [00735] Select culture of either HeLa (adherent human cervical cancer) cells grown in DMEM-10% FBS or BxPC-3 (adherent human pancreas adenocarcinoma) cells grown in 90% RPMI 1640-10% FBS. For plating of cell cultures, trypsinize cells with 0.05% trypsin-EDTA. Assay for viability and cell counting via standard Trypan Blue in phosphate buffered saline (PBS) staining. Dilute cells and seed at 1x105 cells/well in a 6well format. Incubate at 37°C in a 5% CO2 atmosphere for 16 hours or until cells adhere and grow to at least 80% confluency.

[00736] Add labeled prodrug mixture to prodrug experimental wells to achieve final predetermined range of concentrations (e.g., 1 μΜ, 5 μΜ, and 10 μΜ). Add labeled parent drug mixture to parent drug experimental wells to achieve final predetermined range of concentrations (e.g., 1 μΜ, 5 μΜ, and 10 μΜ). Reserve

167 untreated wells for negative control. Incubate cells with experimental treatments for predetermined ranges of time (e.g., 15 minutes, 1 hour, 4 hours, and 8 hours).

P³² Detection and Determination of Prodrug Penetration [00737] To harvest, wash wells 3 times with serum-free media and apply non-denaturing TRIS-HC1 lysis buffer with 1% Triton X100 (Cell Signaling Technology, Inc., Boston, MA) and sonicate briefly. Collect cytosolic and nuclear fractions via standard collection techniques.

[00738] Measurement of drug penetration is performed using standard radiation detection techniques. For detection via scintillation counter, add 50 pL of sample to 5 mL of scintillation cocktail and measure betaemission via liquid scintillation counting. Aliquots of each sample are assayed via Bradford colorimetric assays to normalize radiation counts by total protein concentration.

Example 400. Functional Cell Penetration Assay Using Reporter Gene

Assembly of Fusion Gene Vector and Transfection of Cell Line [00739] When nucleic acid prodrugs are used to inhibit specific gene expression, for example, antisense oligonucleotides or antigene oligonucleotides, a functional penetration assay may be desirable. Culture HeLa (adherent human cervical cancer) cells in DMEM-10% FBS. Clone gene of interest into a commercially available vector such as the Living Colors® Fluorescent Protein Vector, Clontech, Mountain View, CA. Transfection of cells with DNA construct and selection for stable transfectants are performed using standard techniques. The result is constitutive expression of gene of interest and a fluorescent reporter (e.g., the protein AcGFPl).

Nucleic Acid Drugs Inhibiting Specific Gene Expression [00740] Prepare nucleic acid molecules comprising chiral phosphorous moieties and corresponding parent drugs as described herein to disrupt the vector’s gene promoter sequence.

Cell Culture and Penetration Testing [00741] Prepare transfected culture by first trypsinizing cells for plating with 0.05% trypsin-EDTA. Assay for viability and cell counting via standard Trypan Blue in phosphate buffered saline (PBS) staining. Dilute cells and seed at 1x105 cells/well in a 6-well format. Incubate at 37°C in a 5% CO2 atmosphere for 16 hours or until cells adhere and grow to at least 80% confluency.

[00742] Fluorescent signal is first detected 8-12 hours after transfection. Add prodrug mixture to prodrug experimental wells to achieve final predetermined range of concentrations (e.g., 1 μΜ, 5 μΜ, and 10 μΜ). Add parent drug mixture to parent drug experimental wells to achieve final predetermined range of concentrations (e.g., 1 μΜ, 5 μΜ, and 10 μΜ). Reserve untreated wells for negative control. Incubate cells with experimental treatments for predetermined ranges of time (e.g., 15 minutes, 1 hour, 4 hours, and 8 hours).

Reporter Gene Expression Determination of Prodrug Penetration [00743] To harvest, wash each well 3 times with serum-free media and retrypsinize. Measurement of drug penetration is performed using standard fluorescence detection techniques. For qualitative fluorescence 168 measurement with microscopy and quantitative measurement with flow cytometry, use the wavelength that is excitatory for the fluorescent reporter (e.g., 488 nm for AcGFPl).

[00744] While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.

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