Production of fucosylated carbohydrates by enzymatic fucosylation synthesis of sugar nucleotides; and in situ regeneration of gdp-fucose.

THE INVENTION REFERS TO METHODS OF ENZYMATIC PRODUCTION OF CARBOHYDRATES, ESPECIALLY FUCOSILATED CARBOHYDRATES. ALSO PERFECTED SYNTHESIS OF 1 GLYCOSYL IS ANALYZED, USING CHEMICAL AND ENZYMATIC MEDIA. THE PHOSPHORILATE GLYCOSIDES ARE THEN USED TO OBTAIN SUGAR NUCLEOTIDES, WHICH ARE, IN turn, USED AS SUGAR DONORS FOR ACCEPTING CARBOHYDRATE GLYCOSYLATION. ESPECIALLY PREFERRED HERE IS THE USE OF A DESCRIBED METHOD FOR FUCOSILATION.