Droplet-based pyrosequencing
Abstract
The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using discrete droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting biochemical reactions, such as the reagents described for use in nucleic acid amplification protocols, affinity-based assay protocols, sequencing protocols, and protocols for analyses of biological fluids.
Term
0.2 yearsto projected expiry
Projected expiry 11 December 2026, counted from filing; an application has no term until it is granted.
- Priority
- Filed
- Published
- Today
- Projected expiry
Every citation, both ways
| Document | Relation | Office | Category | Cited during | Relevant claims |
|---|---|---|---|---|---|
| EP1418243A2 | Cites | European Patent Office (EPO) | A | Search report | 1-15 |
| US2006021875A1 | Cites | United States of America | X | Search report | 1,7 |
| WO9822625A1 | Cites | World Intellectual Property Organization (WIPO) | A | Search report | 1-18 |
| WO9954730A1 | Cites | World Intellectual Property Organization (WIPO) | XY | Search report | 1-3,7 |
| MARGULIES MARCEL ET AL: "Genome sequencing in microfabricated high-density picolitre reactors.", NATURE 15 SEP 2005 LNKD- PUBMED:16056220, vol. 437, no. 7057, 15 September 2005 (2005-09-15), pages 376 - 380, XP002606440, ISSN: 1476-4687 | Non-patent | – | – | Search report | – |
| RUSSOM AMAN ET AL: "Pyrosequencing in a microfluidic flow-through device.", ANALYTICAL CHEMISTRY 1 DEC 2005 LNKD- PUBMED:16316155, vol. 77, no. 23, 1 December 2005 (2005-12-01), pages 7505 - 7511, XP002606441, ISSN: 0003-2700 | Non-patent | – | – | Search report | – |
231 members in 12 offices
Priority claims21
| Document | Office | Kind | Date |
|---|---|---|---|
| 745059P | United States of America | – | |
| 745039P | United States of America | – | |
| 745058P | United States of America | – | |
| 745073P | United States of America | – | |
| 74505906 | United States of America | P | |
| 74503906 | United States of America | P | |
| 74505806 | United States of America | P | |
| 74507306 | United States of America | P | |
| 745914P | United States of America | – | |
| 745950P | United States of America | – | |
| 74591406 | United States of America | P | |
| 74595006 | United States of America | P | |
| 746797P | United States of America | – | |
| 746801P | United States of America | – | |
| 74679706 | United States of America | P | |
| 74680106 | United States of America | P | |
| 806412P | United States of America | – | |
| 80641206 | United States of America | P | |
| 807104P | United States of America | – | |
| 80710406 | United States of America | P | |
| 2006047481 | United States of America | W |
Members231
| Document | Office | Kind | |
|---|---|---|---|
| US2007241068A1 | United States of America | A1 | |
| US2007242105A1 | United States of America | A1 | |
| US2007242111A1 | United States of America | A1 | |
| US2007243634A1 | United States of America | A1 | |
| CA2680061A1 | Canada | A1 | |
| CA2680532A1 | Canada | A1 | |
| WO2007120240A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2007120241A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2007123908A2 | World Intellectual Property Organization (WIPO) | A2 | |
| US2007275415A1 | United States of America | A1 | |
| US2008006535A1 | United States of America | A1 | |
| US2008038810A1 | United States of America | A1 | |
| US2008044893A1 | United States of America | A1 | |
| US2008044914A1 | United States of America | A1 | |
| US2008050834A1 | United States of America | A1 | |
| US2008053205A1 | United States of America | A1 | |
| CA2680062A1 | Canada | A1 | |
| WO2008051310A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2007120240A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2007120241A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US2008230386A1 | United States of America | A1 | |
| WO2008116209A1 | World Intellectual Property Organization (WIPO) | A1 | |
| WO2007123908A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US7439014B2 | United States of America | B2 | |
| US2008281471A1 | United States of America | A1 | |
| EP2016091A2 | European Patent Office (EPO) | A2 | |
| EP2016189A2 | European Patent Office (EPO) | A2 | |
| EP2021103A2 | European Patent Office (EPO) | A2 | |
| WO2009026339A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2008051310A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2009052348A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2009026339A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US2009155902A1 | United States of America | A1 | |
| CN101472940A | China | A | |
| WO2009052348A3 | World Intellectual Property Organization (WIPO) | A3 | |
| CN101500694A | China | A | |
| EP2016091A4 | European Patent Office (EPO) | A4 | |
| WO2009111769A2 | World Intellectual Property Organization (WIPO) | A2 | |
| HK1127066A1 | Hong Kong, China | A1 | |
| HK1127091A1 | Hong Kong, China | A1 | |
| JP2009534653A | Japan | A | |
| US2009263834A1 | United States of America | A1 | |
| US2009280475A1 | United States of America | A1 | |
| US2009280476A1 | United States of America | A1 | |
| WO2009140373A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2009140671A2 | World Intellectual Property Organization (WIPO) | A2 | |
| US2009291433A1 | United States of America | A1 | |
| EP2126038A1 | European Patent Office (EPO) | A1 | |
| WO2009111769A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US2009311713A1 | United States of America | A1 | |
| WO2010006166A2 | World Intellectual Property Organization (WIPO) | A2 | |
| JP2010503516A | Japan | A | |
| US2010041086A1 | United States of America | A1 | |
| WO2010027894A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2010042637A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2009140373A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2010006166A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US2010116640A1 | United States of America | A1 | |
| US7727723B2 | United States of America | B2 | |
| WO2010027894A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US2010140093A1 | United States of America | A1 | |
| US2010143963A1 | United States of America | A1 | |
| US2010151439A1 | United States of America | A1 | |
| WO2009140671A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2010042637A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US7763471B2 | United States of America | B2 | |
| EP2212683A2 | European Patent Office (EPO) | A2 | |
| US2010221713A1 | United States of America | A1 | |
| US2010258441A1 | United States of America | A1 | |
| US7815871B2 | United States of America | B2 | |
| US7816121B2 | United States of America | B2 | |
| US7822510B2 | United States of America | B2 | |
| US2010279374A1 | United States of America | A1 | |
| US2010291578A1 | United States of America | A1 | |
| EP2126038A4 | European Patent Office (EPO) | A4 | |
| EP2016091B1 | European Patent Office (EPO) | B1 | |
| EP2016189A4This record | European Patent Office (EPO) | A4 | |
| US7851184B2 | United States of America | B2 | |
| AT490971T | Austria | T | |
| ATE490971T1 | Austria | T1 | |
| DE602006018794D1 | Germany | D1 | |
| EP2279405A2 | European Patent Office (EPO) | A2 | |
| EP2021103A4 | European Patent Office (EPO) | A4 | |
| EP2286228A2 | European Patent Office (EPO) | A2 | |
| US7901947B2 | United States of America | B2 | |
| ES2356777T3 | Spain | T3 | |
| US2011091989A1 | United States of America | A1 | |
| US2011097763A1 | United States of America | A1 | |
| US2011100823A1 | United States of America | A1 | |
| US2011104747A1 | United States of America | A1 | |
| US7939021B2 | United States of America | B2 | |
| US2011114490A1 | United States of America | A1 | |
| US2011118132A1 | United States of America | A1 | |
| WO2011084703A2 | World Intellectual Property Organization (WIPO) | A2 | |
| JP2011520449A | Japan | A | |
| US2011180571A1 | United States of America | A1 | |
| US2011186433A1 | United States of America | A1 | |
| US7998436B2 | United States of America | B2 | |
| US2011203930A1 | United States of America | A1 | |
| US8007739B2 | United States of America | B2 |
83 legal events, as 9 offices reported them to INPADOC
Over the term
Point at a mark for the eventEvents
| Event | Code | Office | |
|---|---|---|---|
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Notification of lapseLapsedST | ST | FR | |
| Patent lapsedLapsedMM4A | MM4A | IE | |
| Gb: european patent ceased through non-payment of renewal feeCeasedGBPC | GBPC | EP | |
| Lapsed because of non-payment of the annual feeLapsedMM | MM | NL | |
| Patent ceasedCeasedPL | PL | CH | |
| Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal feeWithdrawnR119 | R119 | DE | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Fee paymentPLFP | PLFP | FR | |
| Fee paymentPLFP | PLFP | FR | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| No opposition filed against granted patent, or epo opposition proceedings concluded without decisionGrantedR097 | R097 | DE | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| No opposition filedOpposition26N | 26N | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| No opposition filed within time limitOppositionORIGINAL CODE: 0009261PLBE | PLBE | EP | |
| Information on the status of an ep patent application or granted ep patentGrantedSTATUS: NO OPPOSITION FILED WITHIN TIME LIMITSTAA | STAA | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Standard patents granted in hong kongGrantedGR | GR | HK | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Deletion acc. to par. 5 (withdrawal of the translation of the ep patent)MK05 | MK05 | AT | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lt: invalidation of european patent or patent extensionLTIE | LTIE | EP | |
| Translation files for an european patent granted for nl, confirming art. 52 par. 1 or 6 of the patents act 1995GrantedT3 | T3 | NL | |
| New agentNV | NV | CH | |
| Dpma publication of mentioned ep patent grantGrantedR096 | R096 | DE | |
| European patents granted designating irelandGrantedFG4D | FG4D | IE | |
| Reference to at number (ep patent enters austrian national phase)REF | REF | AT | |
| European patent takes effect as a national patent in ch/liEP | EP | CH | |
| Designated contracting statesAK | AK | EP | |
| European patent grantedGrantedFG4D | FG4D | GB | |
| (expected) grantORIGINAL CODE: 0009210GRAA | GRAA | EP | |
| Grant fee paidORIGINAL CODE: EPIDOSNIGR3GRAS | GRAS | EP | |
| Request for extension of the european patent (deleted)DAX | DAX | EP | |
| Information provided on ipc code assigned before grantRIC1 | RIC1 | EP | |
| Information provided on ipc code assigned before grantRIC1 | RIC1 | EP | |
| Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOSNIGR1GRAP | GRAP | EP | |
| Supplementary search report drawn up and despatchedA4 | A4 | EP | |
| Requests to designate patent in hong kongDE | DE | HK | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Information on inventor provided before grant (corrected)RIN1 | RIN1 | EP | |
| Request for examination filed17P | 17P | EP | |
| Designated contracting statesAK | AK | EP | |
| Request for extension of the european patentAX | AX | EP | |
| Public reference made under article 153(3) epc to a published international application that has entered the european phaseORIGINAL CODE: 0009012PUAI | PUAI | EP |
Numbers
- Publication
- 2016189
- Application
- 68475888
Titles3
- German
- PYROSEQUENZIERUNG AUF TRÖPFCHENBASIS
- English
- DROPLET-BASED PYROSEQUENCING
- French
- PYROSÉQUENÇAGE À BASE DE GOUTTELETTES
Classification
- CPC, 25
- B01L3/502792
- B01L3/502715
- B01L3/502761
- B01L7/52
- B01L7/525
- B01L2200/027
- B01L2200/0668
- B01L2300/0654
- B01L2300/0816
- B01L2300/0861
- B01L2300/0864
- B01L2300/0867
- B01L2300/089
- B01L2300/1827
- B01L2400/0406
- B01L2400/0415
- B01L2400/043
- B01L2400/0436
- B01L2400/0442
- B01L2400/0448
- C12Q1/6869
- G01N33/49
- B01F31/65
- B01F33/3021
- B01F33/3031
- IPC, 2
- C12Q1 68
- C07H21 00
Designated states31
- Contracting states, 31
- Austria
- Belgium
- Bulgaria
- Switzerland
- Cyprus
- Czechia
- Germany
- Denmark
- Estonia
- Spain
- Finland
- France
- United Kingdom
- Greece
- Hungary
- Ireland
- Iceland
- Italy
- Liechtenstein
- Lithuania
- Luxembourg
- Latvia
- Monaco
- Netherlands (Kingdom of the)
and 7 moreShow fewer
- Poland
- Portugal
- Romania
- Sweden
- Slovenia
- Slovakia
- Türkiye