Dyeing composition containing a thiol/disulphide fluorescent colorant comprising a heterocycle, with an external cationic charge, and method for lightening keratin materials using said colorant

0001The invention relates to the coloring of keratin materials using fluorescent thiol / disulfide dyes comprising a heterocycloalkyl group.

0002It is known to dye keratin fibers, in particular human fibers, by direct coloring. The method conventionally used in direct dyeing consists in applying to the keratin fibers direct dyes which are colored and coloring molecules having an affinity for the fibers, in letting them diffuse, then in rinsing the fibers.

0003The direct dyes which are conventionally used are, for example, dyes of the benzene nitro type, anthraquinone dyes, nitropyridines, dyes of the azo, xanthene, acridine, azine or triarylmethane type.

0004The colorings which result from the use of direct dyes are temporary or semi-permanent dyes because the nature of the interactions which bind the direct dyes to the keratin fiber, and their desorption from the surface and / or from the core of the fiber are responsible. their low dye power and their poor resistance to washing or perspiration.

0005Furthermore, the coloring of keratin fibers from conventional direct dyes does not make it possible to lighten the keratin fibers significantly.

0006The lightening of the color of keratin fibers, more particularly dark towards lighter shades, possibly modifying the shade thereof, constitutes an important demand.

0007Conventionally, to obtain a lighter coloring, a chemical bleaching process is used. This method consists in treating keratin materials such as keratin fibers, in particular the hair, with a strong oxidizing system, generally consisting of hydrogen peroxide associated or not with persalts, most often in an alkaline medium.

0008This bleaching system has the drawback of degrading keratin materials, in particular keratin fibers, in particular human fibers such as the hair, and altering their cosmetic properties. The fibers tend to become rough, more difficult to disentangle and more fragile. Finally, the lightening or discoloration of keratin fibers by oxidizing agents is incompatible with treatments for modifying the shape of said fibers, particularly in hair straightening treatments.

0009Another lightening technique is to apply fluorescent direct dyes to dark hair. This technique described in particular in documents<patcit id="pcit0001" dnum="FR2830189"><text>FR 2830189</text></patcit> and <patcit id="pcit0002" dnum="WO2004091473A"><text>WO 2004/091473</text></patcit> respects the quality of the keratin fiber during the treatment, but the fluorescent dyes used do not have satisfactory resistance to shampooing.

0010To increase the toughness of direct dyes, it is known to fix direct dyes by covalent bonding to the hair. For example, it is known to react dyes with reactive groups with very numerous cystine or cysteine ​​residues in keratin fibers, see for example<nplcit id="ncit0001" npl-type="s"><text>Journal of the Society of Dyers and Colourists, Guise and Stapleton, 91, 259-264 (1975</text></nplcit>); <nplcit id="ncit0002" npl-type="s"><text>Journal of Cosmetic Chemistry, 42, 1-17 (1991</text></nplcit>); <patcit id="pcit0003" dnum="CA2024509"><text>CA 2024509</text></patcit>.

0011In addition, it is known to protect the thiol function or functions contained in a molecule to be grafted to the hair before applying them to said hair. <patcit id="pcit0004" dnum="WO9951194A"><text>WO 99/51194</text></patcit>. However, this application does not mention the use of fluorescent dyes for coloring or lightening hair.

0012Other disulfide dyes known for coloring keratin fibers are disulfide derivatives of aminothiophenol derivatives. Such dyes are described for example in the patent<patcit id="pcit0005" dnum="FR1156407"><text>FR 1156407</text></patcit>. These dyes can be used in relatively mild conditions, in the presence of a slightly reducing medium or after a reducing pretreatment of the hair. However, these dyes can cause color changes during application.

0013Finally, the document <patcit id="pcit0006" dnum="WO2005097051A"><text>WO 2005/097051</text></patcit> describes aza-imidazolium disulfide dyes for the direct dyeing of keratin fibers.

0014The object of the present invention is to provide new systems for coloring keratin materials, in particular human keratin fibers, in particular the hair, which do not have the drawbacks of existing bleaching methods. In particular, one of the aims of the present invention is to provide direct coloring systems making it possible to obtain lightening effects in particular on naturally or artificially dark keratin fibers, tenacious in the face of successive shampoos, which do not degrade the keratin fibers and which do not alter their cosmetic properties.

0015This object is achieved with the present invention which relates to a process for dyeing keratin materials, in particular keratin fibers, in particular human fibers such as the hair, more particularly dark hair, consisting in applying to the keratin materials, a dye composition. , comprising in a suitable cosmetic medium, at least one fluorescent dye with heterocycle group, chosen from the dyes of formula <b>(I), (II)</b> or <b>(III)</b> next :<chemistry id="chem0001" num="0001"><img file="EP2004754B1_D0001.tif" /></chemistry>their organic or mineral acid salts, optical isomers, geometric isomers, and solvates such as hydrates; formulas <b>(I), (II)</b> or <b>(III)</b> in which :<ul id="ul0001" list-style="none" compact="compact"><li>➢ R_at and R '_at, identical or different, represent an aryl group (C₁-VS₄) alkyl or a group (C₁-VS₆) alkyl optionally substituted by a hydroxy or amino group, (C₁-VS₄) alkylamino, (C₁-VS₄) dialkylamino, said alkyl radicals being able to form, with the nitrogen atom carrying them, a heterocyle comprising from 5 to 7 members, optionally comprising another heteroatom different or not from nitrogen; preferably R_at and R '_at represent a group (C₁-VS₃) alkyl optionally substituted by a hydroxy group such as methyl or ethyl, or a benzyl group;</li><li>➢R '_vs represents a hydrogen atom, an aryl group (C₁-VS₄) alkyl, a group C₁-VS₆ optionally substituted alkyl;</li><li>➢ R_g, R '_g, R "_g, R "'_g, R_h, R '_h, R "_h and R "'_h, identical or different, represent a hydrogen atom, a halogen atom, an amino group, (C₁-VS₄) alkylamino, (C₁-VS₄) dialkylamino, cyano, carboxy, hydroxy, trifluoromethyl, an acylamino radical, C alkoxy₁-VS₄, (poly) hydroxyalkoxy in C₂-VS₄, alkylcarbonyloxy alkoxycarbonyl, alkylcarbonylamino, an acylamino, carbamoyl, alkylsulfonylamino radical, an amino-sulfonyl radical, or a (C₁-VS₄) alkyl optionally substituted by a group chosen from (C₁-VS₄) alkoxy, hydroxy, cyano, carboxy, amino, (C₁-VS₄) alkylamino and (C₁-VS₄) dialkylamino, or else the two alkyl radicals carried by the nitrogen atom of the amino group form a heterocycle comprising from 5 to 7 members and optionally comprising another heteroatom identical or different to that of the nitrogen atom; particularly R_g, R '_g, R "_g, R "'_g, R_h, R '_h, R "_h and R "'_h represent a hydrogen atom;</li><li>➢ or two R groups_g and R '_g; R "_g and R "'_g; R_h and R '_h ; R "_h and R "'_h carried by two adjacent carbon atoms, together form a benzo, indeno ring, a fused heterocycloalkyl or fused heteroaryl group; the benzo, indeno, heterocycloalkyl or heteroaryl ring being optionally substituted by a halogen atom, an amino group, (C₁-VS₄) alkylamino, (C₁-VS₄) dialkylamino, cyano, carboxy, hydroxy, trifluoromethyl, an acylamino radical, C alkoxy₁-VS₄, (poly) hydroxyalkoxy in C₂-VS₄, alkylcarbonyloxy alkoxycarbonyl, alkylcarbonylamino, an acylamino, carbamoyl, alkylsulfonylamino radical, an amino-sulfonyl radical, or a (C₁-VS₄) alkyl optionally substituted by: a group chosen from (C₁-VS₄) alkoxy, hydroxy, cyano, carboxy, amino, (C₁-VS₄) alkylamino, (C₁-VS₄) dialkylamino, or else the two alkyl radicals carried by the nitrogen atom of the amino group form a heterocycle comprising from 5 to 7 members and optionally comprising another heteroatom identical or different to that of the nitrogen atom; particularly R_g and R '_g ; R "_g and R "'_g together form a benzo group;</li><li>➢ R_i, R '_i; R ", and R" '_i, identical or different, represent a hydrogen atom, or a group (C₁-VS₄) alkyl; particularly R_i, R '_i,; R "_i, and R "'_i, represent a hydrogen atom;</li><li>➢ R₁, R₂, R₃, R₄, R '₁, R '₂, R '₃ and R '₄, identical or different, represent a hydrogen atom or a group (C₁-VS₄) alkyl, (C₁-VS₄) alkoxy, hydroxy, cyano, carboxy, amino, (C₁-VS₄) alkylamino, (C₁-VS₄) dialkylamino, said alkyl radicals being able to form, with the nitrogen atom carrying them, a heterocyle comprising from 5 to 7 members, optionally comprising another heteroatom different or not from nitrogen; including R₁, R₂, R₃, R₄, R '₁, R '₂, R '₃ and R '₄, are hydrogen atoms or an amino group; particularly R₁, R₂, R₃ and R₄ represent a hydrogen atom;</li><li>➢ T_at or T_b, identical or different represent:<ol id="ol0001" compact="compact"><li>i) or a covalent bond σ,</li><li>ii) either one or more radicals or their combinations chosen from -SO₂-, -O-, -S-, -N (R) -, -N⁺(R) (R °) -, -C (O) -, with R and R °, identical or different, representing a hydrogen atom, a C 1 alkyl radical₁-VS₄, C-hydroxyalkyl₁-VS₄ or an aryl (C₁-VS₄) alkyl; including T_at and T_b represent a covalent bond σ or a group chosen from -N (R) -, -C (O) -, -C (O) -N (R) -, -N (R) -C (O) -, -C (O) -N (R) -C (O) -, -OC (O) -, -C (O) -O- and -N⁺(R) (R °) -, with R, R ° identical or different representing a hydrogen atom, a group C₁-VS₄ alkyl; particularly T_at and T_b represent a σ bond;</li><li>iii) or a heterocycloalkyl or heteroaryl radical, cationic or not, preferably monocyclic, containing one or two heteroatoms, particularly two nitrogen atoms, and comprising in particular from 5 to 7 chain members such as imidazolium, piperazinyl, piperazinium, homo-piperazinyl , homopiperazinium optionally substituted by a group (C₁-VS₄) alkyl such as methyl;<chemistry id="chem0002" num="0002"><img file="EP2004754B1_D0002.tif" /></chemistry><chemistry id="chem0003" num="0003"><img file="EP2004754B1_D0003.tif" /></chemistry>identical or different, represent a heterocyclic group, cationic or not, optionally substituted; in particular the heterocycle is monocyclic, saturated, and comprises in total one to two nitrogen atoms and from 5 to 8 members;</li></ol></li><li>➢ m, m ', n and n', identical or different, represent an integer inclusive between 0 and 6 with m + n and m '+ n', identical or different, represent an integer inclusive between 1 and 10; particularly the sum m + n = m '+ n' is an integer inclusive between 2 and 4; preferably when T_at and T_b represent a covalent bond σ, m + n = m '+ n' is an integer equal to 2;</li><li>➢ M 'representing an anionic counterion; and</li><li>➢ Y represents: i) a hydrogen atom; ii) an alkali metal; iii) an alkaline earth metal; iv) an ammonium group: N⁺R^αR^β3R^γR^δ or a phosphonium group: P⁺R^αR^βR^γR^δ with R^α, R^β, R^γ and R^δ, identical or different, representing a hydrogen atom or a group (C₁-VS₄) alkyl; or v) a protective group of thiol function;</li></ul>it being understood that when the compound of formula (I), (II) or (III) contains other cationic parts, it is found associated with one or more anionic counter-ions making it possible to achieve the electroneutrality of the formula (I ), (II) or (III).

0016Another subject of the invention is a dye composition comprising, in an appropriate cosmetic medium at least one fluorescent dye comprising a heterocyclic group of formula (I), (II) or (III) as defined above, and optionally a reducing agent .

0017The invention also relates to new fluorescent dyes of formula (I), (II) or (III) as defined above.

0018The dyeing process according to the invention makes it possible to visibly color dark keratin materials, in particular dark human keratin fibers, in particular dark hair.

0019In addition, the process of the invention makes it possible to obtain a coloration of keratin materials, in particular human keratin fibers, especially the hair, without degrading said material, which is persistent with regard to shampoos, common attacks (sun, perspiration). , and other hair treatments. The method of the invention also makes it possible to obtain a lightening of keratin materials such as keratin fibers, particularly dark keratin fibers and more particularly dark hair.

0020For the purposes of the invention, the term “dark keratinous material” is understood to mean that which has an luminescence L * encrypted in the CIE system L * a * b *, less than or equal to 45 and preferably less than or equal to 40, knowing moreover that L * = 0 is equivalent to black and L * = 100 to white.

0021For the purposes of the invention, the term “naturally or artificially dark hair” means hair whose pitch is less than or equal to 6 (dark blonde) and preferably less than or equal to 4 (chestnut).

0022The lightening of the hair is evaluated by the variation in "pitch" before and after application of the compound of formula (I), (II) or (III).

0023The notion "tone" is based on the classification of natural shades, a tone separating each shade from the one that immediately follows or precedes it. This definition and classification of natural shades is well known to hair professionals and published in the book "<nplcit id="ncit0003" npl-type="b"><text>Hair treatment science ”by Charles ZVIAK 1988, Ed. Masson, p. 215 and 278</text></nplcit>.

0024The pitch of the tone ranges from 1 (black) to 10 (very light blonde), a unit corresponding to a tone; the higher the number, the lighter the shade.

0025An artificially colored hair is a hair whose color has been modified by a coloring treatment, for example coloring with direct dyes or oxidation dyes.

0026Preferably, the composition must, after application to hair, for example brown, lead to the results below.<ul id="ul0002" list-style="dash" compact="compact"><li>We are interested in the reflectance performance of hair when it is irradiated with visible light in the wavelength range from 400 to 700 nanometers.</li><li>The reflectance curves are then compared as a function of the wavelength, of hair treated with the composition of the invention and of untreated hair.</li><li>The curve corresponding to the treated hair must show a reflectance in the wavelength range from 450 to 700 nanometers greater than the curve corresponding to the untreated hair.</li><li>This means that, in the wavelength range from 450 to 700 nanometers, there is at least one range where the reflectance curve corresponding to the treated hair is greater than the reflectance curve corresponding to the untreated hair. The term "superior" means a difference of at least 0.05% of reflectance, and preferably of at least 0.1%. This does not prevent that it can exist in the wavelength range from 450 to 700 nanometers, at least one range where the reflectance curve corresponding to the treated hair is superimposable, or less than the reflectance curve corresponding to the untreated hair.</li></ul>

0027Preferably, the wavelength where the difference is maximum between the reflectance curve of the treated hair and that of the untreated hair, is in the wavelength range from 450 to 650 nanometers, and preferably in the wavelength range from 450 to 620 nanometers.

0028Within the meaning of the present invention, and unless a different indication is given:<ul id="ul0003" list-style="dash" compact="compact"><li>the “aryl” or “heteroaryl” radicals or the aryl or heteroaryl part of a radical can be substituted with at least one substituent carried by a carbon atom, chosen from:<ul id="ul0004" list-style="bullet" compact="compact"><li>a C 1 alkyl radical₁-VS₁₆, preferably in C₁-VS₈, optionally substituted by one or more radicals chosen from hydroxy, C alkoxy radicals₁-VS₂, (poly) -hydroxyalkoxy in C₂-VS₄, acylamino, amino substituted by two alkyl radicals, identical or different, at C₁-VS₄ , optionally carrying at least one hydroxy group or, the two radicals being able to form, with the nitrogen atom to which they are attached, a heterocycle comprising from 5 to 7 members, preferably from 5 to 6 members, optionally saturated or unsaturated substituted optionally comprising another heteroatom identical or different from nitrogen;</li><li>a halogen atom such as chlorine, fluorine or bromine;</li><li>a hydroxy group;</li><li>a C alkoxy radical₁-VS₂ ;</li><li>a C alkylthio radical₁-VS₂ ;</li><li>a (poly) -hydroxyalkoxy radical in C₂-VS₄ ;</li><li>an amino radical;</li><li>a 5- or 6-membered heterocycloalkyl radical;</li><li>an optionally cationic 5 or 6-membered heteroaryl radical, preferably imidazolium, and optionally substituted by a radical (C₁-VS₄) alkyl, preferably methyl;</li><li>an amino radical substituted by one or two identical or different C 1 alkyl radicals₁-VS₆ possibly carrying at least:<ol id="ol0002" compact="compact"><li>i) a hydroxy group,</li><li>ii) an amino group optionally substituted by one or two C 1 alkyl radicals₁-VS₃ optionally substituted, said alkyl radicals being able to form, with the nitrogen atom to which they are attached, a heterocycle comprising from 5 to 7 members, saturated or unsaturated optionally substituted optionally comprising at least one other heteroatom different or not from nitrogen,</li></ol></li><li>an acylamino radical (-NR-COR ') in which the radical R is a hydrogen atom, a C 1 alkyl radical₁-VS₄ optionally carrying at least one hydroxy group and the radical R 'is a C 1 alkyl radical₁-VS₂ ; a carbamoyl radical ((R)₂N-CO-) in which the radicals R, which may or may not be identical, represent a hydrogen atom, a C 1 alkyl radical₁-VS₄ optionally carrying at least one hydroxy group; an alkylsulfonylamino radical (R'SO₂-NR-) in which the radical R represents a hydrogen atom, a C 1 alkyl radical₁-VS₄ optionally carrying at least one hydroxy group and the radical R ′ represents a C 1 alkyl radical₁-VS₄, a phenyl radical; an aminosulfonyl radical ((R)₂N-SO₂-) in which the radicals R, which may or may not be identical, represent a hydrogen atom, a C 1 alkyl radical₁-VS₄ optionally carrying at least one hydroxy group,</li><li>a carboxylic radical in acid or salified form (preferably with an alkali metal or ammonium, substituted or not);</li><li>a cyano group;</li><li>a polyhaloalkyl group comprising from 1 to 6 carbon atoms and from 1 to 6 halogen atoms, which may be identical or different, the polyhaloalkyl group is for example trifluoromethyl;</li></ul></li><li>the cyclic or heterocyclic part of a non-aromatic radical can be substituted by at least one substituent carried by a carbon atom chosen from the groups:<ul id="ul0005" list-style="bullet" compact="compact"><li>hydroxy,</li><li>C alkoxy₁-VS₄,</li><li>(poly) hydroxyalkoxy in C₂-VS₄,</li><li>a C alkylthio radical₁-VS₂ ;</li><li>alkylcarbonylamino (RCO-NR'-) in which the radical R 'is a hydrogen atom, a C 1 alkyl radical₁-VS₄ optionally carrying at least one hydroxy group and the radical R is a C 1 alkyl radical₁-VS₂, amino substituted by two identical or different C alkyl groups₁-VS₄ optionally carrying at least one hydroxy group, said alkyl radicals being able to form, with the nitrogen atom to which they are attached, a heterocycle comprising from 5 to 7 members, saturated or unsaturated optionally substituted optionally comprising at least one other different heteroatom or not nitrogen;</li><li>alkylcarbonyloxy (RCO-O-) in which the radical R is a C 1 alkyl radical₁-VS₄, amino substituted by two identical or different C alkyl groups₁-VS₄ optionally carrying at least one hydroxy group, said alkyl radicals being able to form, with the nitrogen atom to which they are attached, a heterocycle comprising from 5 to 7 members, saturated or unsaturated optionally substituted optionally comprising at least one other different heteroatom or not nitrogen;</li><li>alkoxycarbonyl (RO-CO-) in which the radical R is a C 1 alkyl radical₁-VS₄, amino substituted by two identical or different C alkyl groups₁-VS₄ optionally carrying at least one hydroxy group, said alkyl radicals being able to form, with the nitrogen atom to which they are attached, a heterocycle comprising from 5 to 7 members, saturated or unsaturated optionally substituted optionally comprising at least one other different heteroatom or not nitrogen;</li></ul></li><li>a cyclic, heterocyclic radical, or a non-aromatic part of an aryl or heteroaryl radical, can also be substituted by one or more oxo or thioxo groups;</li><li>an “aryl” radical represents a mono or polycyclic group, condensed or not, comprising from 6 to 22 carbon atoms, and of which at least one ring is aromatic; preferably the aryl radical is a phenyl, biphenyl, naphthyl, indenyl, anthracenyl, or tetrahydronaphthyl;</li><li>a “diarylalkyl” radical represents a group comprising on the same carbon atom of an alkyl group two aryl groups, identical or different, such as diphenylmethyl or 1,1-diphenylethyl;</li><li>a “heteroaryl radical” represents a mono or polycyclic group, condensed or not, optionally cationic, comprising from 5 to 22 links, from 1 to 6 heteroatoms chosen from the nitrogen, oxygen, sulfur and selenium atom, and of which at least one cycle is aromatic; preferably heteroaryl is selected from acridinyl, benzimidazolyl, benzobistriazolyl, benzopyrazolyl, benzopyridazinyle, benzoquinolyl, benzothiazolyl, benzotriazolyl, benzoxazolyl, pyridinyl, tetrazolyl, dihydrothiazolyl, imidazopyridinyl, imidazolyl, indolyl, isoquinolyl, naphthoimidazolyle, naphthooxazolyle, naphthopyrazolyle, oxadiazolyl, oxazolyl, oxazolopyridyl , phenazinyl, phenooxazolyl, pyrazinyl, pyrazolyl, pyrilyl, pyrazoyltriazyle, pyridyle, pyridinoimidazolyle, pyrrolyle, quinolyle, tétrazolyle, thiadiazolyle, thiazolyle, thiazolopyridinyle, thiazoylimidazolyle, thiopyrylyl, triazolyle, xanthylyl and its ammonium salt;</li><li>a “diheteroarylalkyl” radical represents a group comprising on the same carbon atom of an alkyl group two heteroaryl groups, identical or different such as difurylmethyl, 1,1-difurylethyl, dipyrrolylmethyl, dithienylmethyl;</li><li>a “cyclic radical” is a non-aromatic, mono or polycyclic cycloalkyl radical, condensed or not, containing from 5 to 22 carbon atoms, which may contain from 1 to several unsaturations; in particular the cyclic radical is a cyclohexyl;</li><li>a “sterically hindered cyclic” radical is a cyclic radical, aromatic or not, substituted or not, hindered by steric effect or constraint, comprising from 6 to 14 links, which can be bridged, as sterically hindered radicals, mention may be made of bicyclo [ 1.1.0] butane, mesytyles such as 1,3,5-trimethylpnenyl, 1,3,5-tnterbutylphenyl, 1,3,5-isobutylphenyl, 1,3,5-trimetylsillylphenyl and adamantyl;</li><li>a “heterocyclic radical” is a mono or polycyclic nonaromatic radical, condensed or not, containing from 5 to 22 links, comprising from 1 to 6 heteroatoms chosen from the nitrogen, oxygen, sulfur and selenium atom;</li><li>an “alkyl radical” is a C hydrocarbon radical₁-VS₁₆, linear or branched, preferably in C₁-VS₈ ;</li><li>the expression “optionally substituted” attributed to the alkyl radical means that said alkyl radical may be substituted by one or more radicals chosen from the i) hydroxy, ii) alkoxy radicals₁-VS₄, iii) acylamino, iv) amino optionally substituted by one or two alkyl radicals, identical or different, at C₁-VS₄, said alkyl radicals being able to form, with the nitrogen atom which carries them, a heterocycle comprising from 5 to 7 members, optionally comprising another heteroatom + different or not from nitrogen; v) or a quaternary ammonium group -N⁺R'R "R" ', M^- for which R ', R ", R"', identical or different, represent a hydrogen atom, or a C 1 alkyl group₁-VS₄, or else -N R'R "R" 'forms a heteroaryl such as imidazolium optionally substituted by a group C₁-VS₄ alkyl, and M^- represents the counterion of the organic, mineral acid or of the corresponding halide;</li><li>an “alkylene chain” represents a divalent C chain₁-VS₁₈ ; particularly in C₁-VS₆, more particularly in C₁-VS₂ when the chain is linear; optionally substituted by one or more, identical or different, halogen atoms or groups chosen from hydroxy, alkoxy, (di) (C₁-VS₄) (alkyl) amino, R^at-Z^at-C (Z^b) - with Z^at, Z^b, identical or different, representing an oxygen or sulfur atom or an NR group^at', and R^at, representing an alkali metal, a hydrogen atom, or an alkyl group and R^at'representing a hydrogen atom or an alkyl group;</li><li>an "alkoxy radical" is an alkyl-oxy or alkyl-O- radical for which the alkyl radical is a linear or branched C hydrocarbon radical₁-VS₁₆ preferably in C₁-VS₈ ;</li><li>an "alkylthio radical" is an alkyl-S- radical for which the alkyl radical is a hydrocarbon radical, linear or branched, at C₁-VS₁₆ preferably in C₁-VS₈; when the alkylthio group is optionally substituted, this means that the alkyl group is optionally substituted as defined above;</li><li>the limits delimiting the extent of a range of values ​​are included in this range of values;</li><li>a “salt of organic or inorganic acid” is more particularly chosen from a salt derived from i) hydrochloric acid HCl, ii) hydrobromic acid HBr, iii) sulfuric acid H₂SO₄, iv) alkylsulfonic acids: Alk-S (O)₂OH such as methylsulfonic acid and ethylsulfonic acid; v) arylsulfonic acids: Ar-S (O)₂OH such as benzene sulfonic acid and toluene sulfonic acid; vi) citric acid; vii) succinic acid; viii) tartaric acid; ix) lactic acid, x) alkoxysulfinic acids: Alk-OS (O) OH such as methoxysulfinic acid and ethoxysulfinic acid; xi) aryloxysulfinic acids such as tolueneoxysulfinic acid and phenoxysulfinic acid; xii) phosphoric acid H₃PO₄; xiii) acetic acid CH₃COOH; xiv) CF triflic acid₃SO₃H and xv) tetrafluoroboric acid HBF₄ ;</li><li>an “anionic counterion” is an anion or an anionic group associated with the cationic charge of the dye; more particularly the anionic counterion is chosen from i) halides such as chloride, bromide; ii) nitrates; iii) sulfonates including C₁-VS₆ alkylsulfonates: Alk-S (O)₂O- such as methylsulfonate or mesylate and ethylsulfonate; iv) arylsulfonates: Ar-S (O)₂O- such as benzenesulfonate and toluenesulfonate or tosylate; v) citrate; vi) succinate; vii) tartrate; viii) lactate; ix) alkyl sulphates: Alk-OS (O) O- such as methysulphate and ethyl sulphate; x) arylsulfates: Ar-OS (O) O^- such as benzenesulfate and toluenesulfate; xi) alkoxysulfates: Alk-OS (O)₂O^- such as methoxy sulfate and ethoxysulfate; xii) aryloxysulfates: Ar-OS (O)₂O^-, xiii) phosphate; xiv) acetate; xv) triflate; and xvi) borates such as tetrafluoroborate.</li></ul>

0029Heterocyclic group fluorescent dyes of formula (I), (II) or (III) are compounds capable of absorbing in UV or visible radiation at a wavelength λ_abs between 250 and 800 nm and capable of re-emitting in the visible range at an emission wavelength λ_em between 400 and 800 nm.

0030Preferably the fluorescent compounds with a heterocyclic group of the invention are dyes capable of absorbing in the visible λ_abs between 400 and 800 nm and reemitting in the visible λ_em between 400 and 800 nm. More preferably, the fluorescent dyes with a heterocyclic group of formula (I), (II) or (III) are dyes capable of absorbing at a λ_abs between 420 nm and 550 nm and reemitting in the visible at a λ_em between 470 and 600 nm.

0031A particular embodiment of the invention relates to fluorescent dyes with a heterocyclic group of formula (I), (II) or (III) in which m is 0.

0032According to a particular embodiment of the invention, the fluorescent dyes of the invention including the heterocycle of formula:<chemistry id="chem0004" num="0004"><img file="EP2004754B1_D0004.tif" /></chemistry>represents a heterocyclic group<chemistry id="chem0005" num="0005"><img file="EP2004754B1_D0005.tif" /></chemistry>, cationic or not, optionally substituted; in particular the heterocycle is monocyclic, saturated, and comprises a total of two nitrogen atoms and is formed from 5 to 8 members.

0033Particularly the heterocyclic is chosen from piperazinyl, homopiperazinyl, hexahydro-1,4-diazepinyl, and piperazinium, homopiperazinium or hexahydro-1,4-diazepinium optionally substituted by a group (C₁-VS₄) alkyl.

0034According to another embodiment, the thiol fluorescent dyes with a heterocyclic group of formula (I), (II) or (III) including the heterocycle:<chemistry id="chem0006" num="0006"><img file="EP2004754B1_D0006.tif" /></chemistry>represents a heterocyclic group; monocyclic, saturated, comprising in total a single nitrogen atom and formed from 5 to 7 members.

0035Particularly the heterocyclic is chosen from pyrrolidinyl, piperidyl and azepanyl.

0036The fluorescent compounds of the invention of formula (III) contain a SY function which can be in the covalent form -SY or ionic form -S- Y⁺ depending on the nature of Y and the pH of the medium.

0037A particular mode relates to the thiol fluorescent dyes with heterocyclic group of formula (III) with SY function where Y represents a hydrogen atom, or an alkali metal. Advantageously Y represents a hydrogen atom.

0038In accordance with another particular embodiment of the invention, in the above formula (III), Y is a protective group known to those skilled in the art, for example those described in the works <nplcit id="ncit0004" npl-type="b"><text>"Protective Groups in Organic Synthesis", TW Greene, John Willey &amp; Sons ed., NY, 1981, pp.193-217</text></nplcit> ; <nplcit id="ncit0005" npl-type="b"><text>"Protecting Groups", P. Kocienski, Thieme, 3rd ed., 2005, chap. 5</text></nplcit>.

0039Particularly when Y represents a protective group for the thiol function, Y is chosen from the following radicals:<ul id="ul0006" list-style="none" compact="compact"><li>■ (C₁-VS₄) alkylcarbonyl;</li><li>■ (C₁-VS₄) alkylthiocarbonyl;</li><li>■ (C₁-VS₄) alkoxycarbonyl;</li><li>■ (C₁-VS₄) alkoxythiocarbonyl;</li><li>■ (C₁-VS₄) alkylthio-thiocarbonyl;</li><li>■ (di) (C₁-VS₄) (alkyl) aminocarbonyl;</li><li>■ (di) (C₁-VS₄) (alkyl) aminothiocarbonyl;</li><li>■ arylcarbonyl as phenylcarbonyl;</li><li>■ aryloxycarbonyl;</li><li>■ aryl (C₁-VS₄) alkcoxycarbonyl</li><li>■ (di) (C₁-VS₄) (alkyl) aminocarbonyl such as dimethylaminocarbonyl;</li><li>■ (C₁-VS₄) (alkyl) arylaminocarbonyl;</li><li>■ carboxy;</li><li>■ N / A₃^- ; M⁺ with M⁺ representing an alkali metal such as sodium or potassium, or else M 'of formula (III) and M⁺ are absent;</li><li>■ optionally substituted aryl such as phenyl, dibenzosuberyl, or 1,3,5-cycloheptatrienyl,</li><li>■ optionally substituted heteroaryl; including in particular the cationic or non-cationic heteroaryl, comprising from 1 to 4 following heteroatoms:<ol id="ol0003" compact="compact"><li>i) 5-, 6- or 7-membered monocyclics such as furanyl or furyl, pyrrolyl or pyrryl, thiophenyl or thienyl, pyrazolyl, oxazolyl, oxazolium, isoxazolyl, isoxazolium, thiazolyl, thiazolium, isothiazolyl, isothiazolium, 1,2,4-triazol 1,2,4-triazolium, 1,2,3-triazolyle, 1,2,3-triazolium, 1,2,4-oxazolyle, 1,2,4-oxazolium, 1,2,4-thiadiazolyle, 1, 2,4-thiadiazolium, pyrylium, thiopyridyle, pyridinium, pyrimidinyle, pyrimidinium, pyrazinyle, pyrazinium, pyridazinyle, pyridazinium, triazinyl, triazinium, tetrazinyl, tetrazinium, azepine, azepinium, oxazepinyl, oxazepinium, thieepinyl, thieepinium, imidazolyl, imidazolium;</li><li>ii) 8 to 11-membered bicyclics such as indolyl, indolinium, benzoimidazolyl, benzoimidazolium, benzoxazolyl, benzoxazolium, dihydrobenzoxazolinyl, benzothiazolyl, benzothiazolium, pyridoimidazolyl, pyridoimidazolium or thienocycloheptadienyl groups, or thienocycloheptadienic groups) VS₁-VS₄) alkyl such as methyl, or polyhalo (C₁-VS₄) alkyl such as trifluoromethyl;</li><li>iii) or tricyclic <u>ABC</u> following :<chemistry id="chem0007" num="0007"><img file="EP2004754B1_D0007.tif" /></chemistry></li></ol>in which the two cycles <u>AT</u>, <u>VS</u> possibly have a heteroatom, and the cycle <u>B</u> is a 5, 6 or 7-membered ring, particularly a 6-membered ring and contains at least one heteroatom such as pyperidyl, pyranyl;</li><li>■ heterocycloalkyl optionally substituted, optionally cationic, the heterocycloalkyl group represents in particular a 5, 6 or 7-membered saturated or partially saturated monocyclic group comprising from 1 to 4 heteroatoms chosen from oxygen, sulfur and nitrogen, such as di / tetrahydrofuranyl, di / tetrahydrothiophenyl, di / tetrahydropyrrolyle, di / tetrahydropyranyl, di / tetra / hexahydrothiopyranyl, dihydropyridyl, piperazinyl, piperidinyl, tetramethylpiperidinyl, morpholinyl, di / tetra / hexahydroazepinyl, di / tetrahydro-pyrimidinyl these groups being optionally substituted by one or more groups such as (C₁-VS₄) alkyl, oxo or thioxo; or the heterocycle represents the following grouping:<chemistry id="chem0008" num="0008"><img file="EP2004754B1_D0008.tif" /></chemistry>in which R '^vs, R '^d, R '^e, R '^f, R '^g and R '^h, identical or different, represent a hydrogen atom or a group (C₁-VS₄) alkyl, or two groups R '^g with R '^h, and / or R '^e with R '^f form an oxo or thioxo group, or else R '^g with R '^e together form a cycloalkyl; and v represents an integer inclusive between 1 and 3; preferably R '^vs at R '^h represent a hydrogen atom; and an^- represents a counterion;<ul id="ul0007" list-style="none" compact="compact"><li>■ isothiouronium -C (NR '^vsR '^d) = N⁺R '^eR '^f; Year^- with R '^vs , R '^d, R '^e and R '^f, identical or different, represent a hydrogen atom or a group (C₁-VS₄) alkyl; preferably R '^vs at R '^f represent a hydrogen atom; and an^- represents a counterion;</li><li>■ isothiourea -C (NR '^vsR '^d) = NR '^e with R '^vs, R '^d and R '^e as defined above;</li><li>■ (di) aryl (C₁-VS₄) optionally substituted alkyl such as 9-anthracenylmethyl, phenylmethyl or diphenylmethyl optionally substituted by one or more groups in particular chosen from (C₁-VS₄) alkyl, (C₁-VS₄) alkoxy such as methoxy, hydroxy, (C₁-VS₄) alkylcarbonyl, and (di) (C₁-VS₄) (alkyl) amino such as dimethylamino;</li><li>■ (di) heteroaryl (C₁-VS₄) optionally substituted akyl, the heteroaryl group is in particular, cationic or not, monocyclic, comprising 5 or 6 members and from 1 to 4 heteroatoms chosen from nitrogen, oxygen and sulfur, such as the pyrrolyl, furanyl, thiophenyl groups , pyridyl, pyridyl N-oxide such as 4-pyridyl or 2-pyridyl-N-oxide, pyrylium, pyridinium, triazinyl, optionally substituted by one or more groups such as particularly methyl alkyl, advantageously (di) heteroaryl (C₁-VS₄) akyl is (di) heteroaryl-methyl or (di) heteroarylethyl;</li><li>■ CR¹R²R³ with R¹, R² and R³ identical or different, representing a halogen atom or a group chosen from:<ul id="ul0008" list-style="dash" compact="compact"><li>(VS₁-VS₄) alkyl;</li><li>(VS₁-VS₄) alkoxy;</li><li>optionally substituted aryl such as phenyl optionally substituted by one or more groups such as (C₁-VS₄) alkyl, (C₁-VS₄) alkoxy,, hydroxy;</li><li>optionally substituted heteroaryl such as thiophenyl, furanyl, pyrrolyl, pyranyl, pyridyl, optionally substituted by a group (C₁-VS₄) alkyl;</li><li>P (Z¹) R '¹R '²R '³ with R '¹, and R '² identical or different represent a hydroxy group, (C₁-VS₄) alkoxy or alkyl, R '³ represents a hydroxy group or (C₁-VS₄) alkoxy, and Z¹ represents an oxygen or sulfur atom;</li></ul></li></ul></li><li>■ sterically hindered cyclic such as the adamantyl group; and</li><li>■ alkoxy (C₁-VS₄) optionally substituted alkyl such as methoxymethyl (MOM), ethoxyethyl (EOM) and isobutoxymethyl.</li></ul>

0040According to a particular embodiment, the protected thiol fluorescent dyes with a heterocyclic group of formula (III) comprise a group Y i) monocyclic heteroaryl with 5 or 6 aromatic, cationic links comprising from 1 to 4 heteroatoms chosen from oxygen, sulfur and nitrogen, such as oxazolium,, isoxazolium, thiazolium, isothiazolium, 1,2,4-triazolium, 1,2,3-triazolium, 1,2,4-oxazolium, 1,2,4-thiadiazolium, pyrylium, pyridinium , pyrimidinium, pyrazinyl, pyrazinium, pyridazinium, triazinium, tetrazinium, oxazepinium, thieepinyl, thieepinium, imidazolium; ii) cationic 8 to 11-membered bicyclic heteroaryl such as indolinium, benzoimidazolium, benzoxazolium, benzothiazolium, these mono or bicyclic heteroaryl groups being optionally substituted by one or more groups such as alkyl such as methyl, or polyhalo (C₁-VS₄) alkyl such as trifluoromethyl; iii) or following heterocyclic:<chemistry id="chem0009" num="0009"><img file="EP2004754B1_D0009.tif" /></chemistry>in which R '^vs and R '^d, identical or different, represent a hydrogen atom or a group (C₁-VS₄) alkyl; preferably R '^vs at R '^d represent a group (C₁-VS₄) alkyl such as methyl; and an^- represents a counterion.

0041Particularly Y represents a group chosen from oxazolium, isoxazolium, thiazolium, isothiazolium, 1,2,4-triazolium, 1,2,3-triazolium, 1,2,4-oxazolium, 1,2,4-thiadiazolium, pyrylium, pyridinium , pyrimidinium, pyrazinium, pyridazinium, triazinium and imidazolium, benzoimidazolium, benzoxazolium, benzothiazolium, these groups being optionally substituted by one or more groups (C₁-VS₄) alkyl, especially methyl.

0042In particular, the fluorescent dyes of formula (III) comprise a group Y representing an alkali metal or a protective group such as:<ul id="ul0009" list-style="none" compact="compact"><li>➢ (C₁-VS₄) alkylcarbonyl such as methylcarbonyl or ethylcarbonyl;</li><li>➢ arylcarbonyl as phenylcarbonyl;</li><li>➢ (C₁-VS₄) alkoxycarbonyl;</li><li>➢ aryloxycarbonyl;</li><li>➢ aryl (C₁-VS₄) alkoxycarbonyl;</li><li>➢ (di) (C₁-VS₄) (alkyl) aminocarbonyl such as dimethylaminocarbonyl;</li><li>➢ (C₁-VS₄) (alkyl) arylaminocarbonyl;</li><li>➢ optionally substituted aryl such as phenyl;</li><li>➢ 5- or 6-membered monocyclic heteroaryl such as imidazolyl or pyridyl;</li><li>➢ 5-6-membered cationic monocyclic heteroaryl such as pyrylium, pyridinium, pyrimidinium, pyrazinium, pyridazinium, triazinium, imidazolium; these groups being optionally substituted by one or more, identical or different, groups (C₁-VS₄) alkyl such as methyl;</li><li>➢ 8 to 11-membered cationic bicyclic heteroaryl such as benzoimidazolium, or benzoxazolium; these groups being optionally substituted by one or more, identical or different, groups (C₁-VS₄) alkyl such as methyl;</li><li>➢ cationic heterocycle of the following formula:<chemistry id="chem0010" num="0010"><img file="EP2004754B1_D0010.tif" /></chemistry></li><li>➢ isothiouronium -C (NH₂) = N⁺H₂; Year^-;</li><li>➢ isothiourea-C (NH₂) = NH;</li><li>➢ N / A₃^-, M⁺ with M⁺ representing an alkali metal such as sodium or potassium, or else M 'of formula (III) and M⁺ are absent.</li></ul>

0043According to a particular embodiment of the invention, the dyes of the invention belong to the formula <b>(I), (II)</b> or <b>(III)</b>, include the ethylene group connecting the pyridinium part to the phenyl in the ortho or para position or in the 4-4 ', 2-2', 4-2 'or 2-4' positions.

0044Another particular embodiment of the invention relates to disulfide dyes of formula (I) symmetrical ie R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, R₁, R₂, R₃, R₄, T_at, m, n are identical to R '_at, R "_g, R "'_g, R "_h, R "'_h, R "_i, R "'_i, R '₁, R '₂, R '₃, R '₄, T_b, m 'and n' respectively.

0045By way of example of fluorescent dyes of the invention, mention may be made in particular of the following compounds:<tables id="tabl0001" num="0001"><img file="EP2004754B1_D0011.tif" /></tables><tables id="tabl0002" num="0002"><img file="EP2004754B1_D0012.tif" /></tables><tables id="tabl0003" num="0003"><img file="EP2004754B1_D0013.tif" /></tables><tables id="tabl0004" num="0004"><img file="EP2004754B1_D0014.tif" /></tables><tables id="tabl0005" num="0005"><img file="EP2004754B1_D0015.tif" /></tables><tables id="tabl0006" num="0006"><img file="EP2004754B1_D0016.tif" /></tables><tables id="tabl0007" num="0007"><img file="EP2004754B1_D0017.tif" /></tables><tables id="tabl0008" num="0008"><img file="EP2004754B1_D0018.tif" /></tables>

0046With M ', and An^-, identical or different, representing an anionic counterion; and M⁺ representing an alkali metal.

0047The protected thiol dyes of formula (<b>III "</b>) can be synthesized in two stages. The first step is to prepare the unprotected thiol dye (<b>III '</b>) according to methods known to those skilled in the art, for example "<i>Thiols and organic Sulfides</i>", "<i>Thiocyanates and Isothiocyanates, organic</i>", Ullmann's Encyclopedia, Wiley-VCH, Weinheim, 2005. And the second step consisting in protecting the thiol function according to the conventional methods known by a person skilled in the art to lead to the protected thiol dyes of formula (<b>III "</b>). As an example to protect the thiol-SH function of the thiol dye, the methods of the works can be used "<i>Protective Groups in Organic Synthesis</i>", TW Greene, John Willey &amp; Sons ed., NY, 1981, pp.193-217;"<i>Protecting Groups</i>", P. Kocienski, Thieme, 3^th ed., 2005, chap. 5.

0048We can illustrate this method by the method consisting in i) generating heterocyclic thiol fluorescent dyes of formula (<b>III '</b>) by reduction of a fluorescent dye with two chromophores, heterocyclic, carrying a disulfide function -SS- such that (<b>I '</b>) and ii) to protect by conventional methods said thiol function of (<b>III '</b>) to access the protected thiol fluorescent dyes of formula (<b>III "</b>) : <chemistry id="chem0011" num="0011"><img file="EP2004754B1_D0019.tif" /></chemistry>with R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, m, n, Hét and M 'are as defined above; Y 'represents a protective group of thiol function; and R represents a nucleofuge leaving group, such as, for example, mesylate, tosylate, triflate or halide.

0049According to another possibility, a protected thiol compound (b) can be reacted with a protective group Y ′ as defined above prepared according to one of the procedures described in the works cited above, said protected thiol compound comprising at least one nucleophilic function with a sufficient quantity, preferably equimolar, of a "reactive fluorescent chromophore" or of a compound comprising such a "reactive fluorescent chromophore" (<b><u>at</u></b>). In other words (<b><u>at</u></b>) includes an electrophilic function to form a covalent bond Σ as can be schematized below:<chemistry id="chem0012" num="0012"><img file="EP2004754B1_D0020.tif" /></chemistry><chemistry id="chem0013" num="0013"><img file="EP2004754B1_D0021.tif" /></chemistry>with R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, m, n, Hét, Y 'and M' are as defined above; <img file="EP2004754B1_D0022.tif" />representing a nucleophilic group; <img file="EP2004754B1_D0023.tif" />representing an electrophilic group; and Σ the bond generated after attack by the nucleophile on the electrophile.

0050By way of example, the covalent bonds Σ which can be generated are listed in the table below from the condensation of electrophiles with nucleophiles:<tables id="tabl0009" num="0009"><table frame="all"><tgroup cols="3"><colspec colnum="1" colname="col1" colwidth="48mm" /><colspec colnum="2" colname="col2" colwidth="35mm" /><colspec colnum="3" colname="col3" colwidth="42mm" /><thead><row><entry valign="top">Electrophiles <i>E</i></entry><entry valign="top">Nucleophiles <i>Naked</i></entry><entry valign="top">Covalent bonds Σ</entry></row></thead><tbody><row><entry>Esters activated *</entry><entry>Amines</entry><entry>Carboxamides</entry></row><row><entry>Acyl azides **</entry><entry>Amines</entry><entry>Carboxamides</entry></row><row><entry>Acyl halides</entry><entry>Amines</entry><entry>Carboxamides</entry></row><row><entry>Acyl halides</entry><entry>Alcohols</entry><entry>Esters</entry></row><row><entry>Acyl cyanides</entry><entry>Alcohols</entry><entry>Esters</entry></row><row><entry>Acyl cyanides</entry><entry>Amines</entry><entry>Carboxamides</entry></row><row><entry>Alkyl halides</entry><entry>Amines</entry><entry>Alkylamines</entry></row><row><entry>Alkyl halides</entry><entry>Carboxylic acids</entry><entry>Esters</entry></row><row><entry>Alkyl halides</entry><entry>Thiols</entry><entry>Thioesters</entry></row><row><entry>Alkyl halides</entry><entry>Alcohols</entry><entry>Ethers</entry></row><row><entry>Sulfonic acids and their salts</entry><entry>Thiols</entry><entry>Thioethers</entry></row><row><entry>Sulfonic acids and their salts</entry><entry>Carboxylic acids</entry><entry>Esters</entry></row><row><entry>Sulfonic acids and their salts</entry><entry>Alcohols</entry><entry>Ethers</entry></row><row><entry>Anhydrides</entry><entry>Alcohols</entry><entry>Esters</entry></row><row><entry>Anhydrides</entry><entry>Amines</entry><entry>Carboxamides</entry></row><row><entry>Aryl halides</entry><entry>Thiols</entry><entry>Thioethers</entry></row><row><entry>Aryl halides</entry><entry>Amines</entry><entry>Arylamines</entry></row><row><entry>Aziridines</entry><entry>Thiols</entry><entry>Thioethers</entry></row><row><entry>Carboxylic acids</entry><entry>Amines</entry><entry>Carboxamides</entry></row><row><entry>Carboxylic acids</entry><entry>Alcohols</entry><entry>Esters</entry></row><row><entry>Carbodiimides</entry><entry>Carboxylic acids</entry><entry>V-acylureas or anhydrides</entry></row><row><entry>Diazoalkanes</entry><entry>Carboxylic acids</entry><entry>Esters</entry></row><row><entry>Epoxides</entry><entry>Thiols</entry><entry>Thioethers</entry></row><row><entry>Haloacetamides</entry><entry>Thiols</entry><entry>Thioethers</entry></row><row><entry>Imidic esters</entry><entry>Amines</entry><entry>Amidines</entry></row><row><entry>Isocyanates</entry><entry>Amines</entry><entry>Ureas</entry></row><row><entry>Isocyanates</entry><entry>Alcohols</entry><entry>Urethanes</entry></row><row><entry>Isothiocyanates</entry><entry>Amines</entry><entry>Thioureas</entry></row><row><entry>Maleimides</entry><entry>Thiols</entry><entry>Thioethers</entry></row><row><entry>Sulfonic esters</entry><entry>Amines</entry><entry>Alkylamines</entry></row><row><entry>Sulfonic esters</entry><entry>Thiols</entry><entry>Thioethers</entry></row><row><entry>Sulfonic esters</entry><entry>Carboxylic acids</entry><entry>Esters</entry></row><row><entry>Sulfonic esters</entry><entry>Alcohols</entry><entry>Ethers</entry></row><row><entry>Sulfonyl halides</entry><entry>Amines</entry><entry>Sulfonamides</entry></row></tbody></tgroup><tgroup cols="3" rowsep="0"><colspec colnum="1" colname="col1" colwidth="48mm" /><colspec colnum="2" colname="col2" colwidth="35mm" /><colspec colnum="3" colname="col3" colwidth="42mm" /><tbody><row><entry namest="col1" nameend="col3" align="justify"><i>* acted esters of general formula -CO-Part with Part representing a leaving group such as oxysuccinimidyle, oxybenzotriazolyle, optionally substituted aryloxy;</i><i>** the acyl azides can rearrange to give the isocyanates.</i></entry></row></tbody></tgroup></table></tables>

0051A variant of this process is to use a fluorescent chromophore having an electrophilic acrylate function (-OCO-C = C-) on which an addition reaction is carried out which will generate a Σ bond.

0052It is also possible to use a thiol reagent Y'-SH comprising a group Y 'as defined above, the nucleophilic function of SH can react on the carbon atom in alpha of the halogen atom carried by the fluorescent chromophore (<b><u>at</u></b>') to yield the protected thiol fluorescent dye of formula (<b>III</b>"): <chemistry id="chem0014" num="0014"><img file="EP2004754B1_D0024.tif" /></chemistry>with R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, T_at, R_i, R '_i, m, n, Hét, Y ', (III ") and <b>M</b>'are as defined above, and Hal representing a nucleofuge halogen atom such as bromine, iodine or chlorine.

0053More particularly, it is possible to replace a leaving nucleofuge group with a thiourea group (S = C (NRR) NRR) to generate isothiouroniums. For example if the thiourea group is a thioimidazolium (β), the reaction scheme is as follows:<chemistry id="chem0015" num="0015"><img file="EP2004754B1_D0025.tif" /></chemistry>with R_at, R '_vs, R '_d, R_g, R '_g, R_h, R '_h, R_i, R '_i, m, n, Hét, Hal, An^- and M 'are as defined above.

0054A vanante is to use instead of the halide comprising the fluorescent chromophore (<b><u>at'</u></b>) a chromophore comprising another type of nucleofuge such as tosylate, mesylate.

0055In accordance with another possibility, certain fluorescent heterocyclic thiol dyes protected (<b>III</b>"') can be obtained by reacting a protected thiol compound with a compound carrying two carboxylic acid functions activated according to conventional methods (for example reaction with a carbodiimide or with thionyl chloride). The resulting product (<b><u>d</u></b>) is then reacted with a heterocyclic fluorescent chromophore carrying a nucleophilic function (<b><u>vs</u></b>), for example of primary or secondary amine type , or of aliphatic or aromatic alcohol type such as phenol.<chemistry id="chem0016" num="0016"><img file="EP2004754B1_D0026.tif" /></chemistry>with R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, Hét, T_at, Y ', m, n, M', <img file="EP2004754B1_D0023.tif" />, <img file="EP2004754B1_D0022.tif" /> and (III "') as defined above.

0056According to another possibility, the protected heterocyclic thiol fluorescent dyes of formula (<b>III</b>"" ') can be obtained by reaction of a compound comprising a thiol group protected by a group Y', and a hydroxy group activated beforehand in a leaving group nucleofuge (<b><u>of</u></b>), such as for example mesylate, tosylate, triflate or halide with a fluorescent chromophore <u>(<b>vs'</b>)</u> carrying an amino nucleophilic function on the heterocyclic part of Hét.<chemistry id="chem0017" num="0017"><img file="EP2004754B1_D0027.tif" /></chemistry>

0057With R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, Hét, T_at, Y ', n, M', Σ and <i>E</i> are as defined above.

0058By way of example, a compound containing a protected thiol group contains a leaving group nucleofuge R, such as for example mesylate, tosylate, or triflate which can undergo the nucleophilic attack of the amine carried by the fluorescent styrylic chromophore:<chemistry id="chem0018" num="0018"><img file="EP2004754B1_D0028.tif" /></chemistry>

0059Another alternative comes from the use of halides as a leaving nucleofuge group on a thiol compound which can be substituted by a primary amine function, for example carried by a fluorescent styrylic chromophore:<chemistry id="chem0019" num="0019"><img file="EP2004754B1_D0029.tif" /></chemistry>

0060In accordance with another possibility, the protected thiol fluorescent dyes of formula (<b>III</b>) according to the invention can be obtained by reaction of a compound comprising a thiol group Y as defined above and an electrophilic group (<b><u>f</u></b>) with a compound comprising a nucleophilic group. For example, an aldehyde or ketone can be condensed when G represents an oxygen atom with an "activated methylene" such as alkylpyridinium (<b><u>e</u></b>) to generate an ethylene bond> C = C <. This reaction is commonly called "Knoevenagel" condensation. By "activated methylenes" is meant those for example mentioned in the patent<patcit id="pcit0007" dnum="DE19951134"><text>DE19951134</text></patcit> there may be mentioned in particular 1,2-dialkylpyridinium and in particular 1,2-dimethylpyridinium:<chemistry id="chem0020" num="0020"><img file="EP2004754B1_D0030.tif" /></chemistry>with R_1' R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, Hét, T_at, m, n, Y and M 'as defined above and G representing an oxygen or sulfur atom or an N (R) group with R representing a hydrogen atom or a (C₁-VS₄) alkyl.

0061A variant of this process is to carry out i) a condensation of "Knoevenagel" of an aldehyde or of a ketone comprising a heterocycle Het with amino nucleophilic group (<b><u>g</u></b>) with an "activated methylene" such as alkylpyridinium (<b><u>e</u></b>) to lead to the styryl-heterocyclic intermediate (<b><u>h</u></b>). The latter can then ii) react on a cyclic monomer containing at least one thiolactam function, to lead, after opening the ring, to the thiol heterocyclic fluorescent dye (<b>IIIa</b>"""): <chemistry id="chem0021" num="0021"><img file="EP2004754B1_D0031.tif" /></chemistry>

0062With R₁, R₂, R₃, R₄, R_at R_g, R '_g, R_h, R '_h, R_i, R '_i, Het, n and M 'as defined above, G and G', identical or different, an oxygen, sulfur atom or an N (R) group with R representing a hydrogen atom or a (C₁-VS₄) alkyl.

0063Alternatively, when the reagent (<b>g</b>') has a heter group with an exocyclic amino group, the fluorescent dye with amide, thioamide, guanidine or amidine group is obtained<chemistry id="chem0022" num="0022"><img file="EP2004754B1_D0032.tif" /></chemistry>

0064With R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, Hét, n, M ', G et G', as defined above and R 'represents a hydrogen atom, or an alkyl group. It being understood that the amine of the heterocycle of the reagent (<b><u>g</u></b>') can be protected and then unprotected.

0065We can refer to the book <nplcit id="ncit0006" npl-type="b"><text>Advanced Organic Chemistry, J. March, 4th Ed, John Willey &amp; Sons, 1992 or TW Greene "Protective Groups in Organic Synthesis"</text></nplcit>, to have more details on the operating conditions implemented for the processes mentioned above.

0066The thiol fluorescent dyes formed can be transformed into thiol protected fluorescent dyes -SY 'by the protection of thiol -SH using the conventional protective groups.

0067Protected thiol dyes can be unprotected by conventional means.

0068The disulfide dyes of formula <b>(I)</b> can be synthesized in two stages. The first step of preparing the unprotected thiol dye<b>(III)</b> according to the methods described above. And the second step is to oxidize the unprotected thiol dye<b>(III)</b>, to form the disulfide dye of formula <b>(I ')</b> symmetrical. The oxidation of a thiol to disulfide is done according to conventional methods known to those skilled in the art, for example using hydrogen peroxide.<chemistry id="chem0023" num="0023"><img file="EP2004754B1_D0033.tif" /></chemistry>

0069Alternatively, an equivalent of a disulfide compound (<b><u>b "</u></b>) according to one of the procedures described in the works cited above, said disulfide compound comprising two nucleophilic functions with a sufficient quantity of a "reactive fluorescent chromophore" or of a compound comprising such a "reactive fluorescent chromophore" (<b><u>at</u></b>), such as at least two equivalents. In other words (<u><b>at</b>)</u> includes an electrophilic function to form a covalent bond Σ, to lead to the disulfide dye of formula <b>(I ")</b> as can be schematized below:<chemistry id="chem0024" num="0024"><img file="EP2004754B1_D0034.tif" /></chemistry>with R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, m, n, Hét and M 'are as defined above; <i>Naked</i> representing a nucleophilic group; <i>E</i> representing an electrophilic group; and Σ the bond generated after attack by the nucleophile on the electrophile. By way of example, the covalent bonds Σ which can be generated are listed in the table above from the condensation of electrophiles with nucleophiles.

0070In accordance with another possibility, certain disulfide fluorescent dyes <b>(I ")</b> can be obtained by reacting at least two equivalents of a 'reactive fluorescent chromophore' <b><u>(vs)</u></b> comprising a nucleophilic part with an equivalent of a dielectrophilic disulfide compound <b><u>(d ")</u>,</b> to lead to the disulfide dye <b>(I "):</b><chemistry id="chem0025" num="0025"><img file="EP2004754B1_D0035.tif" /></chemistry>with R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i R '_i Hét, m, n, M ', <i>E</i>, <i>Naked</i> and (I ") as defined above.

0071According to another possibility, the heterocyclic disulfide fluorescent dyes of formula <b>(I "')</b> can be obtained by reaction of a compound <b>(d ")</b> comprising a disulfide group, and a hydroxy group activated beforehand in a nucleofuge leaving group <i>Gp,</i> as for example with mesylate, tosylate, triflate or halide with a fluorescent chromophore <b><u>(vs')</u></b> carrying an amino nucleophilic function on the heterocyclic part of Hét.<chemistry id="chem0026" num="0026"><img file="EP2004754B1_D0036.tif" /></chemistry>

0072With R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i, R '_i, Hét, n, M ', and <i>Gp,</i> are as defined above.

0073By way of example, a compound containing a disulfide group contains a leaving group R, such as mesylate, tosylate, or triflate which can undergo the nucleophilic attack of the amine carried by the fluorescent styryl chromophore to lead to the disulfide dye. particular (I "'a):<chemistry id="chem0027" num="0027"><img file="EP2004754B1_D0037.tif" /></chemistry>

0074Another alternative comes from the use of halides as a leaving nucleofuge group on a disulfide compound which can be substituted by a secondary amine function, for example carried by a fluorescent styrylic chromophore:<chemistry id="chem0028" num="0028"><img file="EP2004754B1_D0038.tif" /></chemistry>

0075According to another possibility, the disulfide fluorescent dyes (I) according to the invention can be obtained by reaction of a compound comprising a disulfide group as defined above and an electrophilic group <b><u>(f ")</u></b> with a compound comprising a nucleophilic group. For example, we can condense an aldehyde or ketone when G represents an oxygen atom with an "activated methylene" such as alkylpyridinium.<b><u>(e)</u></b> to generate an ethylene bond> C = C <. This reaction is commonly called "Knoevenagel" condensation. By "activated methylenes" is meant those for example mentioned in the patent<patcit id="pcit0008" dnum="DE19951134"><text>DE19951134</text></patcit> there may be mentioned in particular 1,2-dialkylpyridinium and in particular 1,2-dimethylpyridinium:<chemistry id="chem0029" num="0029"><img file="EP2004754B1_D0039.tif" /></chemistry>with R₁, R₂, R₃, R₄, R_at, R_g, R '_g, R_h, R '_h, R_i R '_i, Hét, T_at, m, n, (I ') and M' as defined above and G representing an oxygen or sulfur atom or an N (R) group with R representing a hydrogen atom or a (C₁-VS₄) alkyl.

0076We can refer to the book<nplcit id="ncit0007" npl-type="b"><text> Advanced Organic Chemistry, J. March, 4th Ed, John Willey &amp; Sons, 1992 or TW Greene "Protective Groups in Organic Synthesis"</text></nplcit><i>,</i> to have more details on the operating conditions implemented for the processes mentioned above.

0077The disymmetric disulfide dyes of formula <b>(I)</b> can be synthesized in one step by reacting an unprotected fluorescent thiol dye with a fluorescent thiol dye protected by Y 'to form the disulfide dye of formula <b>(I).</b><chemistry id="chem0030" num="0030"><img file="EP2004754B1_D0040.tif" /></chemistry>with R_at, R '_at, R_g, R '_g, R "_g, R "'_g, R_h, R '_h, R "_h, R "'_h, R_i, R '_i, R "_i, R "'_i, m, m ', n, n', T_at, T_b, Hét Hét ', (I) and M' are as defined above; Y 'represents a protective group of thiol function.

0078The asymmetric disulfide dyes of formula <b>(II)</b> can be synthesized in one step by reacting a protected thiol fluorescent dye with an unprotected thiol compound to form the disulfide dye of formula <b><u>(i)</u>.</b><chemistry id="chem0031" num="0031"><img file="EP2004754B1_D0041.tif" /></chemistry>with R_at, R '_at, R_g, R '_g, R "_g, R "'_g, R_h, R '_h, R "_h, R "'_h, R_i, R '_i, R "_i, R "'_i, R '_vs, m, m ', n, n', T_at, T_b, Hét, Hét ', (II) and M' are as defined above; Y 'represents a protective group of thiol function.

0079The composition of the invention contains at least one heterocyclic fluorescent dye of formula (I), (II) or (III). In addition to the presence of at least one fluorescent dye of formula (I), (II) or (III), the composition of the invention may also contain a reducing agent. This reducing agent can be chosen from thiols, for example cysteine, homocysteine, thiolactic acid, the salts of these thiols, phosphines, bisulfite, sulfites, thioglycolic acid, as well as its esters, in particular the glycerol monothioglycolate, and thioglycerol. This reducing agent can also be chosen from borohydrides and their derivatives, such as for example the salts of borohydride, cyanoborohydride, triacetoxyborohydride, trimethoxyborohydride: sodium salts, lithium, potassium, calcium, quaternary ammoniums (tetramethylammonium, tetraethylammonium, tetra n -butylammonium, benzyltriethylammonium); catechol-borane.

0080The dye composition useful in the invention generally contains an amount of heterocyclic fluorescent dye of formula (I), (II) or (III) of between 0.001 and 50% relative to the total weight of the composition. Preferably, this amount is between 0.005 and 20% by weight and even more preferably between 0.01 and 5% by weight relative to the total weight of the composition.

0081The dye composition may also contain additional direct dyes. These direct dyes are, for example, chosen from neutral, acidic or cationic benzene nitro direct dyes, neutral, acidic or cationic azo direct dyes, tetraazapentamethine dyes, quinonic dyes and in particular neutral, acidic or cationic anthraquinone dyes, direct azine dyes , triarylmethane direct dyes, indoamine direct dyes and natural direct dyes.

0082Among the natural direct dyes, mention may be made of lawsone, juglone, alizarin, purpurin, carminic acid, kermesic acid, purpurogallin, protocatechaldehyde, indigo, isatin, curcumin, spinulosin, apigenidine. One can also use extracts or decoctions containing these natural dyes and in particular poultices or extracts based on henna.

0083The dye composition may contain one or more oxidation bases and / or one or more couplers conventionally used for dyeing keratin fibers.

0084Among the oxidation bases, mention may be made of para-phenylenediamines, bis-phenylalkylenediamines, para-aminophenols, bis-para-aminophenols, ortho-aminophenols, heterocyclic bases and their addition salts

0085Among these couplers, mention may in particular be made of meta-phenylenediamines, meta-aminophenols, meta-diphenols, naphthalene couplers, heterocyclic couplers and their addition salts.

0086The coupler (s) are each generally present in an amount of between 0.001 and 10% by weight of the total weight of the dye composition, preferably between 0.005 and 6%.

0087The oxidation base (s) present in the dye composition are generally present each in an amount between 0.001 to 10% by weight of the total weight of the dye composition, preferably between 0.005 and 6% by weight.

0088In general, the addition salts of the oxidation bases and of the couplers which can be used in the context of the invention are in particular chosen from addition salts with an acid such as hydrochlorides, hydrobromides, sulfates, citrates, succinates, tartrates, lactates, tosylates, benzenesulfonates, phosphates and acetates and addition salts with a base such as alkali metal hydroxides such as soda, potash, ammonia, amines or alkanolamines.

0089The medium suitable for dyeing, also called dye support, is a cosmetic medium generally consisting of water or a mixture of water and at least one organic solvent. As organic solvent, mention may, for example, be made of lower C alkanols.₁-VS₄, such as ethanol and isopropanol; polyols and polyol ethers such as 2-butoxyethanol, propylene glycol, propylene glycol monomethyl ether, monoethyl ether and diethylene glycol monomethyl ether, as well as aromatic alcohols such as benzyl alcohol or phenoxyethanol, and mixtures thereof.

0090The solvents when they are present are preferably present in proportions preferably of between 1 and 40% by weight approximately relative to the total weight of the dye composition, and even more preferably between 5 and 30% by weight approximately.

0091Alternatively, the invention contains a reducing agent capable of reducing the disulfide bonds of keratin. This reducing agent is as defined above.

0092The dye composition may also contain various adjuvants conventionally used in compositions for dyeing hair, such as anionic, cationic, nonionic, amphoteric, zwitterionic surfactants or mixtures thereof, anionic, cationic, nonionic, amphoteric, zwitterionic polymers or their mixtures, mineral or organic thickening agents, and in particular associative anionic, cationic polymer thickeners, nonionic and amphoteric, antioxidant agents, penetration agents, sequestering agents, perfumes, buffers, dispersing agents, conditioning agents such as, for example, volatile or non-volatile silicones, modified or not, such as silicones amines, film-forming agents, ceramides, preserving agents, opacifying agents, conductive polymers.

0093The above adjuvants are generally present in an amount for each of them of between 0.01 and 20% by weight relative to the weight of the composition.

0094Of course, those skilled in the art will take care to choose this or these optional additional compounds in such a way that the advantageous properties intrinsically attached to the dye composition according to the invention are not, or not substantially, affected by the or the additions envisaged.

0095The pH of the dye composition is generally between 3 and 14 approximately, and preferably between 5 and 11 approximately. It can be adjusted to the desired value by means of acidifying or basifying agents usually used in dyeing keratin fibers or even using conventional buffer systems.

0096Among the acidifying agents, there may be mentioned, by way of example, mineral or organic acids such as hydrochloric acid, orthophosphoric acid, sulfuric acid, carboxylic acids such as acetic acid, tartaric acid, citric acid, lactic acid, sulfonic acids.

0097Among the basifying agents, there may be mentioned, by way of example, ammonia, alkali carbonates, alkanolamines such as mono-, di- and triethanolamines as well as their derivatives, sodium or potassium hydroxides and compounds of following formula (y):<chemistry id="chem0032" num="0032"><img file="EP2004754B1_D0042.tif" /></chemistry>in which W_at is a propylene residue optionally substituted by a hydroxy group or a C 1 alkyl radical₁-VS₄ ; R_a1, R_a2, R_a3 and R_a4, identical or different, represent a hydrogen atom, a C 1 alkyl radical₁-VS₄ or C hydroxyalkyl₁-VS₄.

0098The dye composition may be in various forms, such as in the form of a liquid, cream, gel, or in any other form suitable for dyeing keratin fibers, and in particular hair.

0099According to a particular embodiment in the process of the invention, a reducing agent can be applied as a pretreatment before the application of the composition containing at least one heterocyclic fluorescent dye of formula (I), (II) or (III) .

0100This reducing agent can be chosen from thiols, for example cysteine, homocysteine, thiolactic acid, the salts of these thiols, phosphines, bisulfite, sulfites, thioglycolic acid, as well as its esters, in particular the glycerol monothioglycolate, and thioglycerol. This reducing agent can also be chosen from borohydrides and their derivatives, such as for example the salts of borohydride, cyanoborohydride, triacetoxyborohydride, trimethoxyborohydride: sodium salts, lithium, potassium, calcium, quaternary ammoniums (tetramethylammonium, tetraethylammonium, tetra n -butylammonium, benzyltriethylammonium); catecholborane.

0101This pretreatment can be of short duration, in particular from 0.1 second to 30 minutes, preferably from 1 minute to 15 minutes with a reducing agent as mentioned above.

0102When the heterocyclic thiol fluorescent dye (III) comprises a group Y which protects the thiol function, the process of the invention can be preceded by a deprotection step aimed at restoring <i>in-situ</i> the SH function.

0103For example, it is possible to deprotect the SY function with Y protective group by adjusting the pH as follows:<tables id="tabl0010" num="0010"><table frame="all"><tgroup cols="2"><colspec colnum="1" colname="col1" colwidth="123mm" /><colspec colnum="2" colname="col2" colwidth="23mm" /><thead><row><entry valign="top">Y: protecting group</entry><entry valign="top">protection</entry></row></thead><tbody><row><entry /><entry /></row><row><entry>alkylcarbonyl,</entry><entry>pH> 9</entry></row><row><entry>arylcarbonyl,</entry><entry>pH> 9</entry></row><row><entry>alkoxycarbonyl,</entry><entry>pH> 9</entry></row><row><entry>aryloxycarbonyl,</entry><entry>pH> 9</entry></row><row><entry>arylalkoxycarbonyl</entry><entry>pH> 9</entry></row><row><entry>(di) (alkyl) aminocarbonyl,</entry><entry>pH> 9</entry></row><row><entry>(alkyl) arylaminocarbonyl</entry><entry>pH> 9</entry></row><row><entry>optionally substituted aryl such as phenyl,</entry><entry>pH> 9</entry></row><row><entry>5-, 6- or 7-membered monocyclic heteroaryl such as oxazolium;</entry><entry>pH> 9</entry></row><row><entry>8 to 11 membered bicyclic heteroaryl such as benzoimidazolium, or benzoxazolium</entry><entry>pH> 9</entry></row></tbody></tgroup></table></tables>

0104The deprotection step can also be carried out during a hair pretreatment step such as, for example, the reducing hair pretreatment.

0105Alternatively, the reducing agent is added to the dye composition containing at least one heterocyclic fluorescent dye (I), (II) or (III) at the time of use.

0106According to another method, the composition comprising at least one heterocyclic fluorescent dye of formula (I), (II) or (III) also contains at least one reducing agent as defined above. This composition is then applied to the hair.

0107According to another vanante, the reducing agent is applied in post-treatment, after the application of the composition containing at least one fluorescent dye of formula (I), (II) or (III). The duration of the post-treatment with the reducing agent can be short, for example from 0.1 second to 30 minutes, preferably from 1 minute to 15 minutes, with a reducing agent as described above. According to a particular embodiment, the reducing agent is an agent of the thiol or borohydride type as described above.

0108In the coloring process another variant is to apply the fluorescent dye of formula (I) or (II) at the same time as the reducing agent.

0109A particular embodiment of the invention relates to a process in which the fluorescent dye of formula (I), (II) or (III) can be applied directly to the hair without reducing agents, free from reducing pre or post-treatment.

0110A treatment with an oxidizing agent may possibly be combined. Any type of conventional oxidizing agent can be used in the field. Thus, it can be chosen from hydrogen peroxide, urea peroxide, alkali metal bromates, persalts such as perborates and persulfates, as well as the enzymes among which mention may be made of peroxidases, oxido- 2 electron reductases such as uricases and 4 electron oxygenases such as laccases. The use of hydrogen peroxide is particularly preferred.

0111This oxidizing agent can be applied to the fibers before or after the application of the composition containing at least one fluorescent dye of formula (I), (II) or (III).

0112The application of the dye composition according to the invention is generally carried out at room temperature. It can however be carried out at temperatures varying from 20 to 180 ° C.

0113The invention also relates to a device with several compartments or "kit" for dyeing in which a first compartment contains a dye composition comprising at least one heterocyclic fluorescent dye of formula (I), (II) or (III) and a second compartment contains a reducing agent capable of reducing the disulfide functions of keratin materials.

0114One of these compartments may also contain one or more other dyes of the direct dye or oxidation dye type.

0115It also relates to a device with several compartments in which a first compartment contains a dye composition comprising at least one heterocyclic thiol fluorescent dye of formula (I), (II) or (III); a second compartment contains a reducing agent capable of reducing the disulfide bond of the keratin materials and / or the disulfide bond of the dyes of formula (I) or (II); a third compartment contains an oxidizing agent.

0116Alternatively, the dyeing device contains a first compartment containing a dye composition which comprises at least one protected thiol fluorescent dye of formula (III) and a second compartment containing an agent capable of deprotecting the protected thiol to release the thiol.

0117Each of the devices mentioned above can be equipped with a means enabling the desired mixture to be delivered to the hair, for example such as the devices described in the patent. <patcit id="pcit0009" dnum="FR2586913"><text>FR2 586 913</text></patcit>.

0118The examples which follow serve to illustrate the invention without, however, being limiting in nature. The fluorescent dyes of the examples below were fully characterized by conventional spectroscopic and spectrometric methods.

EXAMPLES

EXAMPLES OF SYNTHESIS

<u>Example 1:</u> Synthesis of 4,4 'dimethyl sulfate - {disulfanediylbis [ethane-2,1-diyl-piperazine-4,1-diyl-4,1-phenylene (E) ethene-2,1-diyl]} bis (1-methylpyridinium ) [1]

0119<chemistry id="chem0033" num="0033"><img file="EP2004754B1_D0043.tif" /></chemistry><chemistry id="chem0034" num="0034"><img file="EP2004754B1_D0044.tif" /></chemistry>

Step 1: Synthesis of 4,4 '- [disulfanediylbis (ethane-2,1-diylpiperazine-4,1-diyl)] - dibenzaldehyde

01202.5g 4-piperazin-1-yl-benzaldehyde, 1.37g 1-bromo-2 - [(2-bromoethyl) -disulfanyl] ethane, 7.3g potassium carbonate (K₂CO₃), and 20 mL of N-methylpyrrolidinone (NMP) are mixed at 75 ° C for 55 h. The product is purified by chromatography on silica gel (eluent: acetone / heptane 80/20 then dichlormethane / methanol 90/10). 469 mg of yellow oil are collected. Analysis indicates that the product is compliant.

Step 2: Synthesis of the 4,4 'dimethyl sulfate - {disulfanediylbis [ethane-2,1-diyl-piperazine-4,1-diyl-4,1-phenylene (E) ethene-2,1-diyl]} bis ( 1-methylpyridinium) [1]

0121469 4.4 'mg - [disulfanediylbis (ethane-2,1-diylpiperazine-4,1-diyl)] dibenzaldehyde, 5 mL isopropanol (iPrOH), 415 mg 1,4-dimethylpyridinium methyl sulfate, 80 mg pyrrolidine, 56 mg of acetic acid and 5 mL of dichloromethane are mixed at room temperature for 24 h and then at 40 ° C for 24 h. After cooling the mixture, 20 ml of acetone are added, the precipitate obtained is filtered, washed with 10 ml of isopropanol, then with 3 times 25 ml of acetone, and dried. 610 mg of red powder are collected. Analysis indicates that the product is compliant.

0122NMR¹ H (400 MHz, MeOH<i>-d</i>₄) 2.68 (m, 8 H), 2.78 (t, 4 H), 2.95 (t, 4 H), 3.37 (m, 8 H), 3.68 (s, 6 H), 4.24 (s, 6 H), 7 (d, 4 H), 7.16 (d, 2 H), 7.62 (d, 4 H), 7.82 (d, 2 H), 8.01 (d, 4 H), 8.56 (d, 4 H)

EXAMPLE 2 Synthesis of 1-methyl-4-methyl sulfate ((E) -2- {4- [4- (2-sulfanyl-ethyl) piperazin-1-yl] phenyl} vinyl) pyridinium [2]

0123<chemistry id="chem0035" num="0035"><img file="EP2004754B1_D0045.tif" /></chemistry><chemistry id="chem0036" num="0036"><img file="EP2004754B1_D0046.tif" /></chemistry><chemistry id="chem0037" num="0037"><img file="EP2004754B1_D0047.tif" /></chemistry>

Procedure

Synthesis of the salt of 1-methyl-4 - ((E) -2- {4- [4- (4- (2-sulfanylethyl) piperazin-1-yl] phenyl} vinyl) pyridinium [2]

012416 mg of compound [1] are dissolved in 10 mL of water / ethanol mixture (v / v: 1/1); 60 mg (2 eq.) Of 3- [bis (2-carboxy-ethyl) phosphino] propanoic acid hydrochloride hydrate in solution in 1 ml of water and 21 mg (4 eq) of sodium bicarbonate in solution in 1 mL of water are added to the mixture. After 30 minutes of stirring at 40 ° C under an inert atmosphere, the analyzes indicate that the mixture contains most of the expected product<b>[2].</b>LC-MS analysis: LC-DAD (400-700 nm) Column: Waters XTerra MS C18 5µm (4.6 x 50) mm Mobile phase: A: Water + 0.1% formic acid / B: acetonitrile Linear gradient: T (min) A% / B%: 0 min 95/5; 8 min 0/100 Flow rate: 1 ml / min. Detection: UV Diode array λ = 400-700nm Retention time t = 3.0 min. Relative purity> 90% MS (ESI +) m / z = 285 corresponds to the mass peak of the monocation of the expected product <b>[2]</b>

COLORING EXAMPLE

<u>Example 1:</u> Coloring process - compound [1]

Preparation of composition A

0125<tables id="tabl0011" num="0011"><table frame="all"><tgroup cols="2"><colspec colnum="1" colname="col1" colwidth="73mm" /><colspec colnum="2" colname="col2" colwidth="20mm" /><tbody><row><entry>Disulfide dye [1]</entry><entry align="right">10^-3 mol%</entry></row><row><entry>Benzyl alcohol</entry><entry align="right">4 g</entry></row><row><entry>Polyethylene glycol 6OE</entry><entry align="right">6 g</entry></row><row><entry>Hydroxyethylcellulose</entry><entry align="right">0.7 g</entry></row><row><entry>Alkylpolyglucoside in aqueous solution at 65% MA</entry><entry align="right">4.5g</entry></row><row><entry>Demineralized Water</entry><entry align="right">qs 100g</entry></row></tbody></tgroup></table></tables>

Preparation of a composition B

0126<tables id="tabl0012" num="0012"><table frame="all"><tgroup cols="2"><colspec colnum="1" colname="col1" colwidth="36mm" /><colspec colnum="2" colname="col2" colwidth="20mm" /><tbody><row><entry>Thioglycolic acid</entry><entry align="right">1M</entry></row><row><entry>Sodium hydroxide</entry><entry align="right">qs pH 8.5</entry></row><row><entry>Demineralized Water</entry><entry align="right">qs 100g</entry></row></tbody></tgroup></table></tables>

0127At the time of use, compositions A (9 mL) and B (1 mL) are mixed, then the mixture obtained is applied to a lock of 1 g of dark hair (pitch 4) for 30 minutes at room temperature (the wicks are turned over and re-impregnated after 15 minutes).

0128After rinsing with running water and drying, a lightening of the hair thus treated is observed: the lock of pitch 4 has become visually clearer than untreated control locks.

<u>Example 2</u> : dyeing process with the compound [2]

012910 mL of fresh solution of the compound <b>[2]</b> of the synthetic example are applied to a lock of 1g of hair, tone height 4, placed at the bottom of a bowl for 30 minutes at room temperature (the locks are turned over and re-impregnated after 15 minutes). The locks are then rinsed with running water and dried.

0130After coloring, the pitch of tone 4 became visually clearer than untreated control strands.

Remanence towards successive shampoos:

0131The locks thus treated are divided into two, one half is subjected to 5 successive shampoos according to a cycle which comprises wetting the locks with water, washing with a conventional shampoo, rinsing with water followed by drying.

Visual observations

0132During shampooing, there is no visible bleeding, the foam of the shampoos and the rinsing waters are not colored

0133The observed color is preserved and the lightening effect remains visible on the hair of tone 4 height thus treated.

Results in the L * a * b * system:

0134The color of the locks before and after the 5 washes was evaluated in the L * a * b * system using a CM 2600 KONICA MINOLTA® spectrophotometer, (Illuminant D65).

0135In this system L * a * b *, L * represents the intensity of the color, a * indicates the axis of color green / red and b * the axis of color blue / yellow. The higher the value of L, the lighter or less intense the color. Conversely, the lower the value of L, the darker or very intense the color. The higher the value of a * the more the shade is red and the higher the value of b * the more the shade is yellow.

0136The vanation of the coloring between the locks of HT4 hair, colored and washed is measured by (ΔE) according to the following equation: <maths id="math0001"><math display="block"><mi mathvariant="normal">ΔE</mi><mo mathvariant="normal">=</mo><msqrt><msup><mfenced><mi mathvariant="normal">L</mi><mo mathvariant="normal">*</mo><mo mathvariant="normal">-</mo><msub><mi mathvariant="normal">L</mi><mi mathvariant="normal">o</mi></msub><mo mathvariant="normal">*</mo></mfenced><mn mathvariant="normal">2</mn></msup><mo mathvariant="normal">+</mo><msup><mfenced><mi mathvariant="normal">at</mi><mo mathvariant="normal">*</mo><mo mathvariant="normal">-</mo><msub><mi mathvariant="normal">at</mi><mi mathvariant="normal">o</mi></msub><mo mathvariant="normal">*</mo></mfenced><mn mathvariant="normal">2</mn></msup><mo mathvariant="normal">+</mo><msup><mfenced><mi mathvariant="normal">b</mi><mo mathvariant="normal">*</mo><mo mathvariant="normal">-</mo><msub><mi mathvariant="normal">b</mi><mi mathvariant="normal">o</mi></msub><mo mathvariant="normal">*</mo></mfenced><mn mathvariant="normal">2</mn></msup></msqrt></math><img file="EP2004754B1_D0048.tif" /></maths>

0137In this equation, L *, a * and b * represent the values ​​measured before coloring and L₀*, at₀* and B₀* represent the values ​​measured after coloring or after coloring and shampooing.

0138The greater the value of ΔE, the greater the difference in color between the HT4 locks and the colored locks.<tables id="tabl0013" num="0013"><table frame="all"><tgroup cols="2"><colspec colnum="1" colname="col1" colwidth="111mm" /><colspec colnum="2" colname="col2" colwidth="12mm" /><thead><row><entry valign="top"><b>Treatment with the compound on HT4 wicks</b></entry><entry align="center" valign="top"><b>ΔE</b></entry></row></thead><tbody><row><entry>After application of compound 1 according to the invention</entry><entry align="center">8,84</entry></row><row><entry>After application of compound 1 according to the invention and 5 successive shampoos</entry><entry align="center">7,38</entry></row><row><entry /><entry align="center" /></row><row><entry>After application of compound 2 according to the invention</entry><entry align="center">4,22</entry></row><row><entry>After application of compound 2 according to the invention and 5 successive shampoos</entry><entry align="center">5,24</entry></row></tbody></tgroup></table></tables>

0139The results of the table show that there is no significant difference in color variation even after 5 shampoos. Thus the coloring and the lightening effect on the hair remain almost unchanged, which shows very good resistance to shampooing of the dyes of the invention.

Reflectance Results:

0140The lightening of the compositions in accordance with the invention and their persistence with respect to successive shampoos were also expressed as a function of the reflectance of the hair. These reflectances are compared to the reflectance of a lock of untreated hair of tone height HT4.

0141The reflectance is measured using a KONIKA-MINOLTA® spectrophotocolorimeter, CM 2600d and after irradiation of the hair with visible light in the wavelength range from 400 to 700 nanometers.<img file="EP2004754B1_D0049.tif" /><img file="EP2004754B1_D0050.tif" />

0142It is noted first of all that the reflectance of a lock of hair treated with a composition according to the invention is higher than that of untreated hair. The treated locks therefore appear visually clearer.

0143In addition, the results show that the reflectance of the locks of hair having a tone height 4 treated with the compositions of the invention changes very shortly after 5 shampoos. Thus the coloring and the lightening effect on the hair remain almost unchanged, which shows very good resistance to shampooing of the dyes of the invention.