Gated voltammetry

0001The present invention relates to a voltammetric method for determining the concentration of an analyte and to a handheld measuring device adapted to receive a sensor strip for carrying out the voltammetric method. The method of the invention is defined in claim 1 and the handheld measuring device of the invention is defined in claim 26.

<u>BACKGROUND</u>

0002The quantitative determination of analytes in biological fluids is useful in the diagnosis and treatment of physiological abnormalities. For example, determining the glucose level in biological fluids, such as blood, is important to diabetic individuals who must frequently check their blood glucose level to regulate their diets and/or medication.

0003Electrochemical systems have been used for this type of analysis. During the analysis, the analyte undergoes a redox reaction with an enzyme or similar species to generate an electric current that may be measured and correlated with the concentration of the analyte. A substantial benefit may be provided to the user by decreasing the time required for the analysis while supplying the desired accuracy and precision.

0004One example of an electrochemical sensor system for analyzing analytes in biological fluids includes a measuring device and a sensor strip. The sensor strip includes reagents to react with and transfer electrons from the analyte during the analysis and electrodes to pass the electrons through conductors to the device. The measuring device includes contacts to receive the electrons from the strip and the ability to apply a voltage differential between the contacts. The device may record the current passing through the sensor and translate the current values into a measure of the analyte content of the sample. These sensor systems may analyze a single drop of whole blood (WB), such as from 1-15 microliters (µL) in volume.

0005Examples of bench-top measuring devices include the BAS 100B Analyzer available from BAS Instruments in West Lafayette, Indiana; the CH Instrument Analyzer available from CH Instruments in Austin, Texas; the Cypress Electrochemical Workstation available from Cypress Systems in Lawrence, Kansas; and the EG&amp;G Electrochemical Instrument available from Princeton Research Instruments in Princeton, New Jersey. Examples of portable measuring devices include the Ascensia Breeze® and Elite® meters of Bayer Corporation.

0006The sensor strip may include a working electrode where the analyte undergoes electrochemical reaction and a counter electrode where the opposite electrochemical reaction occurs, thus allowing current to flow between the electrodes. Thus, if oxidation occurs at the working electrode, reduction occurs at the counter electrode. <i>See,</i> for example, <nplcit id="ncit0001" npl-type="b"><text>Fundamentals Of Analytical Chemistry, 4th Edition, D.A. Skoog and D.M. West; Philadelphia: Saunders College Publishing (1982), pp 304-341</text></nplcit>.

0007The sensor strip also may include a true reference electrode to provide a non-variant reference potential to the measuring device. While multiple reference electrode materials are known, a mixture of silver (Ag) and silver chloride (AgCl) is typical due to the insolubility of the mixture in the aqueous environment of the analysis solution. A reference electrode also may be used as the counter electrode. A sensor strip using such a combination reference-counter electrode is described in <patcit id="pcit0001" dnum="US5820551A"><text>U.S. Pat. No. 5,820,551</text></patcit>.

0008The sensor strip may be formed by printing electrodes on an insulating substrate using multiple techniques, such as those described in <patcit id="pcit0002" dnum="US6531040B"><text>U.S. Pat. Nos. 6,531,040</text></patcit>; <patcit id="pcit0003" dnum="US5798031A"><text>5,798,031</text></patcit>; and <patcit id="pcit0004" dnum="US5120420A"><text>5,120,420</text></patcit>. One or more reagent layer may be formed by coating one or more of the electrodes, such as the working and/or counter electrodes. In one aspect, more than one of the electrodes may be coated by the same reagent layer, such as when the working and counter electrodes are coated by the same composition. In another aspect, reagent layers having different compositions may be printed or micro-deposited onto the working and counter electrodes using the method described in a <patcit id="pcit0005" dnum="US51381703P" dnum-type="L"><text>U.S. provisional patent application filed October 24, 2003, Serial No. 60/513,817</text></patcit>. Thus, the reagent layer on the working electrode may contain the enzyme, the mediator, and a binder while the reagent layer on the counter electrode contains a soluble redox species, which could be the same as the mediator or different, and a binder.

0009The reagent layer may include an ionizing agent for facilitating the oxidation or reduction of the analyte, as well as any mediators or other substances that assist in transferring electrons between the analyte and the conductor. The ionizing agent may be an analyte specific enzyme, such as glucose oxidase or glucose dehydrogenase, to catalyze the oxidation of glucose in a WB sample. The reagent layer also may include a binder that holds the enzyme and mediator together. Table I, below, provides conventional combinations of enzymes and mediators for use with specific analytes. <tables id="tabl0001" num="0001"><table frame="all"><title><b>Table I</b></title><tgroup cols="3"><colspec colnum="1" colname="col1" colwidth="22mm" /><colspec colnum="2" colname="col2" colwidth="40mm" /><colspec colnum="3" colname="col3" colwidth="32mm" /><thead><row><entry valign="top"><b>Analyte</b></entry><entry valign="top"><b>Enzyme</b></entry><entry valign="top"><b>Mediator</b></entry></row></thead><tbody><row rowsep="0"><entry>Glucose</entry><entry>Glucose Oxidase</entry><entry>Ferricyanide</entry></row><row rowsep="0"><entry>Glucose</entry><entry>Glucose Dehydrogenase</entry><entry>Ferricyanide</entry></row><row rowsep="0"><entry>Cholesterol</entry><entry>Cholesterol Oxidase</entry><entry>Ferricyanide</entry></row><row rowsep="0"><entry>Lactate</entry><entry>Lactate Oxidase</entry><entry>Ferricyanide</entry></row><row rowsep="0"><entry>Uric Acid</entry><entry>Uricase</entry><entry>Ferricyanide</entry></row><row><entry>Alcohol</entry><entry>Alcohol Oxidase</entry><entry>Phenylenediamine</entry></row></tbody></tgroup></table></tables>

0010The binder may include various types and molecular weights of polymers, such as CMC (carboxyl methyl cellulose) and/or PEO (polyethylene oxide). In addition to binding the reagents together, the binder may assist in filtering red blood cells, preventing them from coating the electrode surface.

0011Examples of conventional electrochemical sensor systems for analyzing analytes in biological fluids include the Precision® biosensors available from Abbott in Abbott Park, Illinois; Accucheck® biosensors available from Roche in Indianapolis, Indiana; and OneTouch Ultra® biosensors available from Lifescan in Milpitas, California.

0012One electrochemical method, which has been used to quantify analytes in biological fluids, is coulometry. For example, Heller et al. described the coulometric method for WB glucose measurements in <patcit id="pcit0006" dnum="US6120676A"><text>U.S. Pat. No. 6,120,676</text></patcit>. In coulometry, the analyte concentration is quantified by exhaustively oxidizing the analyte within a small volume and integrating the current over the time of oxidation to produce the electrical charge representing the analyte concentration. Thus, coulometry captures the total amount of analyte present within the sensor strip.

0013An important aspect of coulometry is that towards the end of the integration curve of charge vs. time, the rate at which the current changes with time becomes substantially constant to yield a steady-state condition. This steady-state portion of the coulometric curve forms a relatively flat plateau region, thus allowing determination of the corresponding current. However, the coulometric method requires the complete conversion of the entire volume of analyte to reach the steady-state condition. As a result, this method is time consuming and does not provide the fast results which users of electrochemical devices, such as glucose-monitoring products, demand. Another problem with coulometry is that the small volume of the sensor cell must be controlled in order to provide accurate results, which can be difficult with a mass produced device.

0014Another electrochemical method which has been used to quantify analytes in biological fluids is amperometry. In amperometry, current is measured during a read pulse as a constant potential (voltage) is applied across the working and counter electrodes of the sensor strip. The measured current is used to quantify the analyte in the sample. Amperometry measures the rate at which the electrochemically active species, such as the analyte, is being oxidized or reduced near the working electrode. Many variations of the amperometric method for biosensors have been described, for example in <patcit id="pcit0007" dnum="US5620579A"><text>U.S. Pat. Nos. 5,620,579</text></patcit>; <patcit id="pcit0008" dnum="US5653863A"><text>5,653,863</text></patcit>.; <patcit id="pcit0009" dnum="US6153069A"><text>6,153,069</text></patcit>; and <patcit id="pcit0010" dnum="US6413411B"><text>6,413,411</text></patcit>.

0015A disadvantage of conventional amperometric methods is the non-steady-state nature of the current after a potential is applied. The rate of current change with respect to time is very fast initially and becomes slower as the analysis proceeds due to the changing nature of the underlying diffusion process. Until the consumption rate of the reduced mediator at the electrode surface equals the diffusion rate, a steady-state current cannot be obtained. Thus, for conventional amperometry methods, measuring the current during the transient period before a steady-state condition is reached may be associated with more inaccuracy than a measurement taken during a steady-state time period.

0016The "hematocrit effect" provides an impediment to accurately analyzing the concentration of glucose in WB samples. WB samples contain red blood (RB) cells and plasma. The plasma is mostly water, but contains some proteins and glucose. Hematocrit is the volume of the RB cell constituent in relation to the total volume of the WB sample and is often expressed as a percentage. Whole blood samples generally have hematocrit percentages ranging from 20% to 60%, with ∼40% being the average.

0017In conventional sensor strips, glucose may be oxidized by an enzyme, which then transfers the electron to a mediator. This reduced mediator then travels to the working electrode where it is electrochemically oxidized. The amount of mediator being oxidized may be correlated to the current flowing between the working and counter electrodes of the sensor strip. Quantitatively, the current measured at the working electrode is directly proportional to the diffusion coefficient of the mediator. The hematocrit effect interferes with this process because the RB cells block the diffusion of the mediator to the working electrode. Subsequently, the hematocrit effect influences the amount of current measured at the working electrode without any connection to the amount of glucose in the sample.

0018WB samples having varying concentrations of RB cells may cause inaccuracies in the measurement because the sensor may not distinguish between a lower mediator concentration and a higher mediator concentration where the RB cells block diffusion to the working electrode. For example, when WB samples containing identical glucose levels, but having hematocrits of 20, 40, and 60%, are analyzed, three different glucose readings, will be reported by a conventional sensor system based on one set of calibration constants (slope and intercept, for instance). Even though the glucose concentrations are the same, the system will report that the 20% hematocrit sample contains more glucose than the 60% hematocrit sample due to the RB cells interfering with diffusion of the mediator to the working electrode.

0019The normal hematocrit range (RBC concentration) for humans is from 20% to 60% and is centered around 40%. Hematocrit bias refers to the difference between the reference glucose concentration obtained with a reference instrument, such as the YSI 2300 STAT PLUS™ available from YSI Inc., Yellow Springs, Ohio, and an experimental glucose reading obtained from a portable sensor system for samples containing differing hematocrit levels. The difference between the reference and experimental readings results from the varying hematocrit levels between specific WB samples.

0020In addition to the hematocrit effect, measurement inaccuracies also may arise when the measurable species concentration does not correlate with the analyte concentration. For example, when a sensor system determines the concentration of a reduced mediator generated in response to the oxidation of an analyte, any reduced mediator not generated by oxidation of the analyte will lead to the sensor system indicating that more analyte is present in the sample than is correct due to mediator background.

0021In addition to the hematocrit and mediator background effects, other factors also may lead to inaccuracies in the ability of a conventional electrochemical sensor system to determine the concentration of an analyte in a sample. In one aspect, these inaccuracies may be introduced because the portion of the sensor strip that contains the sample may vary in volume from strip to strip. Inaccuracies also may be introduced when sufficient sample is not provided to completely fill the volume of the cap-gap, a condition referred to as under-fill. In other aspects, inaccuracies may be introduced into the measurement by random "noise" and when the sensor system lacks the ability to accurately determine temperature changes in the sample.

0022In an attempt to overcome one or more of these disadvantages, conventional sensor systems have attempted multiple techniques, not only with regard to the mechanical design of the sensor strip and reagent selection, but also regarding the manner in which the measuring device applies the electric potential to the strip. For example, conventional methods of reducing the hematocrit effect for amperometric sensors include the use of filters, as disclosed in <patcit id="pcit0011" dnum="US5708247A"><text>U.S. Pat. Nos. 5,708,247</text></patcit> and <patcit id="pcit0012" dnum="US5951836A"><text>5,951,836</text></patcit>; reversing the polarity of the applied current, as disclosed in <patcit id="pcit0013" dnum="WO0157510A"><text>WO 01/57510</text></patcit>; and by methods that maximize the inherent resistance of the sample, as disclosed in <patcit id="pcit0014" dnum="US5628890A"><text>U.S. Pat. No. 5,628,890</text></patcit>.

0023Multiple methods of applying the electric potential to the strip, commonly referred to as pulse methods, sequences, or cycles, have been used to address inaccuracies in the determined analyte concentration. For example, in <patcit id="pcit0015" dnum="US4897162A"><text>U.S. Pat. No. 4,897,162</text></patcit> the pulse method includes a continuous application of rising and falling voltage potentials that are commingled to give a triangular-shaped wave. Furthermore, <patcit id="pcit0016" dnum="WO2004053476A"><text>WO 2004/053476</text></patcit> and <patcit id="pcit0017" dnum="US20030178322A"><text>U.S. Pat. Documents 2003/0178322</text></patcit> and <patcit id="pcit0018" dnum="US20030113933A"><text>2003/0113933</text></patcit> describe pulse methods that include the continuous application of rising and falling voltage potentials that also change polarity.

0024Other conventional methods combine a specific electrode configuration with a pulse sequence adapted to that configuration. For example, <patcit id="pcit0019" dnum="US5942102A"><text>U.S. Pat. No. 5,942,102</text></patcit> combines the specific electrode configuration provided by a thin layer cell with a continuous pulse so that the reaction products from the counter electrode arrive at the working electrode. This combination is used to drive the reaction until the current change verses time becomes constant, thus reaching a true steady state condition for the mediator moving between the working and counter electrodes during the potential step. While each of these methods balances various advantages and disadvantages, none are ideal. <patcit id="pcit0020" dnum="US4304853A"><text>US 4,304,853</text></patcit> disclose a method for the determination of proteases and antiproteases, according to which a fragment liberated from a substrate by enzymatic hydrolysis is determined electrochemically. In the method of <patcit id="pcit0021" dnum="US4304853A"><text>US 4,304,853</text></patcit> the electrical input signal used to follow the reaction includes voltammetric peaks separated by 60 second amperometric flat portions. The initial, rapidly changing signal is used to pre-condition the electrode, while the later portion of the signal is analytic and uses a peak current recorded during the voltammetric peaks to determine the progress of the reaction. The input signal is continuous, having no breaks in voltage application to the sample. Thus, voltage is always applied during the scan and current is continuously generated. <patcit id="pcit0022" dnum="US4304853A"><text>US 4,304,853</text></patcit> does not describe the effect of this voltage change on the current flow from the samples. The degree and duration of current reduction in the method of <patcit id="pcit0023" dnum="US4304853A"><text>US 4,304,853</text></patcit> is also not described. <patcit id="pcit0024" dnum="US6413398B1"><text>US 6,413,398 B1</text></patcit> discloses a method for electrochemical detection (ECD). The illustrated embodiments are particularly adapted to use in detection of analytes separated by capillary electrophoresis. The method is a voltammetric ECD, including a preparatory positive potential pulse, an optional stabilizing pulse at a negative potential and an analytical pulse at extreme negative potentials (e.g., -300 mV to -1,500 mV), and a positive cleaning potential pulse. The methods have been shown to detect analytes previously considered to be electrochemically inactive using more traditional electrochemical methods. Examples of such analytes include various drugs, antibiotics, and peptides. The analytical pulse comprises a scan across a range of negative potentials and comprises a linear ramp including a superimposed square-wave form with an amplitude of about 100 mV and a frequency of about 1000 Hz. The wave form of the analytical pulse and interpretation of the subsequent current response is taken from square-wave voltammetry (SWV). <patcit id="pcit0025" dnum="US6413398B1"><text>US 6,413,398 B1</text></patcit> therefore discloses an amperometric method of determining an analyte concentration. It does not address the current flow during the reversals of the SWV signal, thus failing to teach the degree and duration of current flow reduction required by relaxations of the present application. <patcit id="pcit0026" dnum="US4897162A"><text>US 4,897,162</text></patcit> discloses a glucose sensing apparatus and method including an electrocatalytic sensor having a reference electrode and a sensing electrode. A periodic signal is comprised of a ramp voltage which is intermingled a series of square wave measurement pulses. This signal is applied to the sensor. Current levels are sampled twice during each measurement pulse, and a signal indicative of glucose level is derived therefrom. After completion of a measurement, a reactivation signal is applied to the electrode to regenerate deteriorated surfaces thereof. <patcit id="pcit0027" dnum="US4897162A"><text>US 4,897,162</text></patcit> fails to address the current flow during the continuous input signal thus failing to teach the degree and duration of current flow reduction required by the relaxations of the present claims. <patcit id="pcit0028" dnum="US5873990A"><text>US 5,873,990</text></patcit> discloses an apparatus which is a microprocessor based instrument designed to conveniently and rapidly measure various analytes in environmental and biological samples. An anodic stripping voltametry (ASV) method that includes a pre-concentration step where a heavy metal is deposited onto an electrode similarly to electroplating is disclosed. The deposited or plated metal is than electrically removed from the electrode. The current required to remove the metal may be correlated with the amount of metal initially deposited. <patcit id="pcit0029" dnum="US5873990A"><text>US 5,873,990</text></patcit> discloses multiple methods of stripping the deposited metal from the electrode with the pre-filled method being square-wave voltammetry (SWV). The device can use SWV to determine the concentration of non-metal analytes such as drugs. According to the method of <patcit id="pcit0030" dnum="US5873990A"><text>US 5,873,990</text></patcit> the applied voltage is incremented from one level to a final level in a series of steps, during each step a small offset voltage is applied, first in the positive direction then in the negative direction. <patcit id="pcit0031" dnum="US5873990A"><text>US 5,873,990</text></patcit> therefore discloses an amperometric method of determining an analyte concentration since the current measurements from which the analyte concentration is determined are taken from a generally uniform voltage level of the input signal. It fails to address the current flow during the continuous input signals used to strip the heavy metal from the electrode, thus failing to teach the degree and duration of current flow reduction required by the relaxations between the excitations.

0025As may be seen from the above description, there is an ongoing need for improved electrochemical sensor systems, especially those that may provide increasingly accurate determination of the analyte concentration in less time. The systems, devices, and methods of the present invention overcome at least one of the disadvantages associated with conventional systems.

<u>SUMMARY</u>

0026A voltammetric method of determining the concentration of an analyte in a sample is provided that includes applying a pulse sequence to the sample and measuring the resulting currents, the pulse sequence includes at least two duty cycles, where each of the duty cycles includes an excitation and a relaxation, the excitation includes voltammetric scanning, and the relaxation is from 0.1 to 3 seconds and includes a current reduction to at least one-half the current flow at the maximum of the excitation. In addition to the at least two duty cycles, the pulse sequence may include a terminal read pulse and/or an initial time delay and may be applied to a sensor strip including a diffusion barrier layer on a working electrode. The method may include less bias attributable to mediator background than a concentration of the analyte determined from another method or from a voltammetric method lacking the pulse sequence comprising at least two duty cycles. The sample may be a liquid including a biological fluid and the analyte may be glucose.

0027The duty cycles may include an excitation including a potential varied with time or a potential varied linearly with time, such as a linear, cyclic, acyclic, or a combination of these excitation types. A current value may be recorded during each of the excitations and the pulse sequence may include a terminal read pulse. The duty cycles may include acyclic excitations substantially excluding a reverse oxidation peak or a reverse reduction peak and may reduce the concentration of a mediator in the sample not responsive to the analyte in relation to the method where the duty cycles comprise cyclic excitations. The duty cycles may include acyclic excitations terminating before initiation of a reverse current peak, acyclic excitations substantially excluding forward and reverse oxidation and reduction peaks, or acyclic excitations substantially within a diffusion limited current region of a redox pair.

0028The method may include the determination of at least one contour profile and may include applying at least one data treatment, such as semi-integral, semi-derivative, or derivative, to the resulting currents. The method also may include determining a plurality of calibration sets from the currents and determining the number of duty cycles from the plurality of calibration sets. Determination of the analyte concentration may include averaging multiple concentration values obtained from the plurality of calibration sets.

0029The method also may include determining if a sensor strip containing the sample is under-filled with the sample. This determination may include comparing at least one current value to a pre-selected value. The method also may include determining the active ionizing agent content of a sensor strip, a determination that may be made by determining a ratio from forward and reverse scan current values. In one aspect, this ratio was previously correlated to known amounts of the active ionizing agent. In another aspect, a calibration slope may be altered in response to the active ionizing agent content of the sensor strip. In another aspect, the excitation/relaxation time ratio of the duty cycles may be from 0.3 to 0.2.

0030A handheld analyte measuring device is provided for determining the concentration of an analyte in a sample. The device includes a gated voltammetric measuring device adapted to receive a sensor strip. The gated amperometric measuring device includes at least two device contacts in electrical communication with a display through electrical circuitry. The sensor strip includes at least first and second sensor strip contacts. The first sensor strip contact is in electrical communication with a working electrode and the second sensor strip contact is in electrical communication with a counter electrode through conductors. A first reagent layer is on at least one of the electrodes and includes an oxidoreductase and at least one species of a redox pair. The electrodes may be on the same or on different substrates.

0031The electronic circuitry may establish electrical communication between the contacts and the display. The circuitry includes an electric charger and a processor, where the processor is in electrical communication with a computer readable storage medium. The medium includes computer readable software code, which when executed by the processor, causes the charger to implement a gated voltammetric pulse sequence including at least two duty cycles, where each of the duty cycles includes an excitation and a relaxation, the excitation includes voltammetric scanning, and the relaxation is from 0.1 to 3 seconds and includes a current reduction to at least one-half the current flow at the maximum of the excitation.

0032A method of reducing the bias attributable to mediator background in a determined concentration of an analyte in a sample is provided that includes applying a gated voltammetric pulse sequence including at least two duty cycles.

0033A method of determining the duration of a pulse sequence including at least 2 duty cycles, for determining the concentration of an analyte in a sample is provided that includes determining a plurality of sets of calibration constants determined from currents recorded during the at least 2 duty cycles and determining the duration of the pulse sequence in response to the determined concentration of the analyte in the sample. The pulse sequence may be a gated voltammetric pulse sequence.

0034A method of signaling a user to add additional sample to a sensor strip is provided that includes determining if the sensor strip is under-filled by comparing at least one current value recorded from a pulse sequence including at least 2 duty cycles to a pre-selected value and signaling the user to add additional sample to the sensor strip if the strip is under-filled. The pulse sequence may be a gated voltammetric pulse sequence. The sensor strip may include two electrodes and the determining may be performed in less than five seconds.

0035A voltammetric method of determining the concentration of an analyte in a sample is provided that includes applying a pulse sequence to the sample and measuring the resulting currents, the pulse sequence includes at least 2 duty cycles having excitation/relaxation time ratios from 0.3 to 0.2. The method may be more accurate than a concentration of the analyte determined from another method where the excitation/relaxation time ratio of a pulse is greater than 0.3.

0036An electrochemical method for determining the concentration of an analyte in a sample is provided that includes an improvement including applying a gated voltammetric pulse sequence to the sample including at least two duty cycles.

0037The following definitions are included to provide a clear and consistent understanding of the specification and claims.

0038The term "analyte" is defined as one or more substances present in a sample. The analysis determines the presence and/or concentration of the analyte present in the sample.

0039The term "sample" is defined as a composition that may contain an unknown amount of the analyte. Typically, a sample for electrochemical analysis is in liquid form, and preferably the sample is an aqueous mixture. A sample may be a biological sample, such as blood, urine, or saliva. A sample also may be a derivative of a biological sample, such as an extract, a dilution, a filtrate, or a reconstituted precipitate.

0040The term "voltammetry" is defined as an analysis method where the concentration of an analyte in a sample is determined by electrochemically measuring the oxidation or reduction rate of the analyte at a varying potential.

0041The term "system" or "sensor system" is defined as a sensor strip in electrical communication through its conductors with a measuring device, which allows for the quantification of an analyte in a sample.

0042The term "sensor strip" is defined as a device that contains the sample during the analysis and provides electrical communication between the sample and the measuring device. The portion of the sensor strip that contains the sample is often referred to as the "cap-gap."

0043The term "conductor" is defined as an electrically conductive substance that remains stationary during an electrochemical analysis.

0044The term "measuring device" is defined as one or more electronic devices that may apply an electric potential to the conductors of a sensor strip and measure the resulting current. The measuring device also may include the processing capability to determine the presence and/or concentration of one or more analytes in response to the recorded current values.

0045The term "accuracy" is defined as how close the amount of analyte measured by a sensor strip corresponds to the true amount of analyte in the sample. In one aspect, accuracy may be expressed in terms of bias.

0046The term "precision" is defined as how close multiple analyte measurements are for the same sample. In one aspect, precision may be expressed in terms of the spread or variance among multiple measurements.

0047The term "redox reaction" is defined as a chemical reaction between two species involving the transfer of at least one electron from a first species to a second species. Thus, a redox reaction includes an oxidation and a reduction. The oxidation half-cell of the reaction involves the loss of at least one electron by the first species, while the reduction half-cell involves the addition of at least one electron to the second species. The ionic charge of a species that is oxidized is made more positive by an amount equal to the number of electrons removed. Likewise, the ionic charge of a species that is reduced is made less positive by an amount equal to the number of electrons gained.

0048The term "mediator" is defined as a substance that may be oxidized or reduced and that may transfer one or more electrons. A mediator is a reagent in an electrochemical analysis and is not the analyte of interest, but provides for the indirect measurement of the analyte. In a simplistic system, the mediator undergoes a redox reaction in response to the oxidation or reduction of the analyte. The oxidized or reduced mediator then undergoes the opposite reaction at the working electrode of the sensor strip and is regenerated to its original oxidation number.

0049The term "binder" is defined as a material that provides physical support and containment to the reagents while having chemical compatibility with the reagents.

0050The term "mediator background" is defined as the bias introduced into the measured analyte concentration attributable to measurable species not responsive to the underlying analyte concentration.

0051The term "measurable species" is defined as any electrochemically active species that may be oxidized or reduced under an appropriate potential at the working electrode of an electrochemical sensor strip. Examples of measurable species include analytes, oxidoreductases, and mediators.

0052The term "under-fill" is defined as when insufficient sample was introduced into the sensor strip to obtain an accurate analysis.

0053The term "redox pair" is defined as two conjugate species of a chemical substance having different oxidation numbers. Reduction of the species having the higher oxidation number produces the species having the lower oxidation number. Alternatively, oxidation of the species having the lower oxidation number produces the species having the higher oxidation number.

0054The term "oxidation number" is defined as the formal ionic charge of a chemical species, such as an atom. A higher oxidation number, such as (III), is more positive, and a lower oxidation number, such as (II), is less positive.

0055The term "reversible redox pair" is defined as a pair of redox species where the separation between the forward and reverse scans of the semi-integral is at most 30 mV at the half-height of the si_ss transition. For example, in <figref idref="f0014">FIG. 10A</figref> the forward and reverse semi-integral scans for the ferricyanide/ferrocyanide redox pair in addition to the si_ss transition height are shown. At the line where the half-height si_ss transition line intersects the forward and reverse scan lines the separation between the lines is 29 mV, establishing the reversibility of the ferricyanide/ferrocyanide redox pair at the depicted scan rate.

0056The term "quasi-reversible redox pair" is defined as a redox pair where the separation between the forward and reverse scans of the semi-integral is larger than 30 mV at the half-height of the si_ss transition for the redox pair.

0057The term "soluble redox species" is defined as a substance that is capable of undergoing oxidation or reduction and that is soluble in water (pH 7, 25° C) at a level of at least 1.0 grams per Liter. Soluble redox species include electro-active organic molecules, organotransition metal complexes, and transition metal coordination complexes. The term "soluble redox species" excludes elemental metals and lone metal ions, especially those that are insoluble or sparingly soluble in water.

0058The term "oxidoreductase" is defined as any enzyme that facilitates the oxidation or reduction of an analyte. An oxidoreductase is a reagent. The term oxidoreductase includes "oxidases," which facilitate oxidation reactions where molecular oxygen is the electron acceptor; "reductases," which facilitate reduction reactions where the analyte is reduced and molecular oxygen is not the analyte; and "dehydrogenases," which facilitate oxidation reactions where molecular oxygen is not the electron acceptor. <i>See, for example,</i><nplcit id="ncit0002" npl-type="b"><text>Oxford Dictionary of Biochemistry and Molecular Biology, Revised Edition, A.D. Smith, Ed., New York: Oxford University Press (1997) pp. 161, 476, 477, and 560</text></nplcit>.

0059The term "electro-active organic molecule" is defined as an organic molecule lacking a metal that is capable of undergoing an oxidation or reduction reaction. Electro-active organic molecules may serve as mediators.

0060The term "organotransition metal complex," also referred to as "OTM complex," is defined as a complex where a transition metal is bonded to at least one carbon atom through a sigma bond (formal charge of -1 on the carbon atom sigma bonded to the transition metal) or a pi bond (formal charge of 0 on the carbon atoms pi bonded to the transition metal). For example, ferrocene is an OTM complex with two cyclopentadienyl (Cp) rings, each bonded through its five carbon atoms to an iron center by two pi bonds and one sigma bond. Another example of an OTM complex is ferricyanide (III) and its reduced ferrocyanide (II) counterpart, where six cyano ligands (formal charge of -1 on each of the 6 ligands) are sigma bonded to an iron center through the carbon atoms.

0061The term "coordination complex" is defined as a complex having well-defined coordination geometry, such as octahedral or square planar. Unlike OTM complexes, which are defined by their bonding, coordination complexes are defined by their geometry. Thus, coordination complexes may be OTM complexes (such as the previously mentioned ferricyanide), or complexes where non-metal atoms other than carbon, such as heteroatoms including nitrogen, sulfur, oxygen, and phosphorous, are datively bonded to the transition metal center. For example, ruthenium hexaamine is a coordination complex having a well-defined octahedral geometry where six NH₃ ligands (formal charge of 0 on each of the 6 ligands) are datively bonded to the ruthenium center. A more complete discussion of organotransition metal complexes, coordination complexes, and transition metal bonding may be found in <nplcit id="ncit0003" npl-type="s"><text>Collman et al., Principles and Applications of Organotransition Metal Chemistry (1987</text></nplcit>) and <nplcit id="ncit0004" npl-type="s"><text>Miessler &amp; Tarr, Inorganic Chemistry (1991</text></nplcit>).

0062The term "steady-state" is defined as when the change in electrochemical signal (current) with respect to its independent input variable (voltage or time) is substantially constant, such as within ±10 or ±5%.

0063The term "relatively constant" is defined as when the change in a current value or a diffusion rate is within ±20, ±10, or ±5%.

0064The term "reversing-point" is defined as the point in a cyclic or acyclic scan when the forward scan is stopped and the reverse scan is initiated.

0065The term "linear excitation" is defined as an excitation where the voltage is varied in a single "forward" direction at a fixed rate, such as from -0.5 V to +0.5 V to provide a 1.0 V excitation range. The excitation range may cover the reduced and oxidized states of a redox pair so that a transition from one state to the other occurs. A linear excitation may be approximated by a series of incremental changes in potential. If the increments occur very close together in time, they correspond to a continuous linear excitation. Thus, applying a change of potential approximating a linear change may be considered a linear excitation.

0066The term "cyclic excitation" is defined as a combination of a linear forward excitation and a linear reverse excitation where the excitation range includes the oxidation and reduction peaks of a redox pair. For example, varying the potential in a cyclic manner from -0.5 V to +0.5 V and back to -0.5 V is an example of a cyclic excitation for the ferricyanide/ferrocyanide redox pair as used in a glucose sensor, where both the oxidation and reduction peaks are included in the excitation range.

0067The term "acyclic excitation" is defined in one aspect as an excitation including more of one forward or reverse current peak than the other current peak. For example, an excitation including forward and reverse linear excitations where the forward excitation is started at a different voltage than where the reverse excitation stops, such as from -0.5 V to +0.5 V and back to +0.25 V, is an example of an acyclic excitation. In another example, an acyclic excitation may start and end at substantially the same voltage when the excitation is started at most ±20, ±10, or ±5 mV away from the formal potential E^∘' of the redox pair. In another aspect, an acyclic excitation is defined as an excitation including forward and reverse linear excitations that substantially exclude the oxidation and reduction peaks of a redox pair. For example, the excitation may begin, reverse, and end within the diffusion-limited region of a redox pair, thus excluding the oxidation and reduction peaks of the pair.

0068The terms "fast excitation," "fast excitation rate," "fast scan," and "fast scan rate" are defined as an excitation where the voltage is changed at a rate of at least 176 mV/sec. Preferable fast excitation rates are rates greater than 200, 500, 1,000, or 2,000 mV/sec.

0069The terms "slow excitation," "slow excitation rate," "slow scan," and "slow scan rate" are defined as an excitation where the voltage is changed at a rate of at most 175 mV/sec. Preferable slow excitation rates are rates slower than 150, 100, 50, or 10 mV/sec.

0070The term "average initial thickness" refers to the average height of a layer prior to the introduction of a liquid sample. The term average is used because the top surface of the layer is uneven, having peaks and valleys.

0071The term "redox intensity" (RI) is defined as the total excitation time divided by the sum of the total excitation time and the total relaxation time delays for a pulse sequence.

0072The term "handheld device" is defined as a device that may be held in a human hand and is portable. An example of a handheld device is the measuring device accompanying Ascensia® Elite Blood Glucose Monitoring System, available from Bayer HealthCare, LLC, Elkhart, IN.

0073The term "on" is defined as "above" and is relative to the orientation being described. For example, if a first element is deposited over at least a portion of a second element, the first element is said to be "deposited on" the second. In another example, if a first element is present above at least a portion of a second element, the first element is said to be "on" the second. The use of the term "on" does not exclude the presence of substances between the upper and lower elements being described. For example, a first element may have a coating over its top surface, yet a second element over at least a portion of the first element and its top coating may be described as "on" the first element. Thus, the use of the term "on" may or may not mean that the two elements being related are in physical contact.

<u>BRIEF DESCRIPTION OF THE DRAWINGS</u>

0074The invention may be better understood with reference to the following drawings and description. The components in the figures are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the invention. Moreover, in the figures, like referenced numerals designate corresponding parts throughout the different views. <ul id="ul0001" list-style="none" compact="compact"><li><figref idref="f0001">FIG. 1A</figref> is a perspective representation of an assembled sensor strip.</li><li><figref idref="f0001">FIG. 1B</figref> is a top-view diagram of a sensor strip, with the lid removed.</li><li><figref idref="f0001">FIG. 2</figref> depicts an end-view diagram of the sensor strip of <figref idref="f0001">FIG. 1B</figref>.</li><li><figref idref="f0002">FIGs. 3A and 3B</figref> depict a working electrode having a surface conductor and a DBL during the application of long and short read pulses.</li><li><figref idref="f0003">FIGs. 4A and 4B</figref> are graphs illustrating the improvement in measurement accuracy when a DBL is combined with a short excitation.</li><li><figref idref="f0004">FIG. 5</figref> represents an electrochemical analytic method of determining the presence and concentration of an analyte in a sample.</li><li><figref idref="f0005 f0006 f0007">FIGs. 6A-6F</figref> represent six examples of pulse sequences where multiple duty cycles were applied to the sensor strip after introduction of the sample, which do not fall under the scope of the independent claims.</li><li><figref idref="f0008">FIG. 7A</figref> is a graph showing a cyclic voltammogram from a sensor system.</li><li><figref idref="f0008">FIG. 7B</figref> compares a cyclic scan to an acyclic scan, where the forward excitation of the acyclic scan was started near the formal potential E°' for the redox pair.</li><li><figref idref="f0009">FIG. 7C</figref> shows an acyclic scan, where the reverse scan is terminated the reverse current peak.</li><li><figref idref="f0009">FIG. 7D</figref> shows a cyclic scan with an acyclic scan superimposed in the DLC region.</li><li><figref idref="f0010 f0011">FIGs. 8A-8D</figref> shows the output currents plotted as voltammograms from the pulse sequence represented in <figref idref="f0006">FIG. 6C</figref> for 40% hematocrit WB samples containing 50, 100, and 400 mg/dL glucose</li><li><figref idref="f0012 f0013">FIGs. 9A-9C</figref> show contour profiles of the voltammograms of <figref idref="f0010 f0011">FIGs. 8A-8C</figref>.</li><li><figref idref="f0014">FIG. 10A</figref> is a graph of the semi-integral corresponding to the cyclic voltammogram of <figref idref="f0008">FIG. 7A</figref>.</li><li><figref idref="f0014">FIG. 10B</figref> presents the semi-integral of the acyclic data corresponding to the acyclic voltammogram of <figref idref="f0009">FIG. 7C</figref>.</li><li><figref idref="f0015">FIG. 10C</figref> presents the semi-integrals of the cyclic and acyclic excitations of <figref idref="f0008">FIG. 7B</figref>.</li><li><figref idref="f0015">FIG. 10D</figref> shows the semi-integral and recorded current values for the acyclic excitation of <figref idref="f0009">FIG. 7D</figref>.</li><li><figref idref="f0016">FIG. 11</figref> shows contour profiles prepared by semi-integrating voltammograms from a seven excitation pulse sequence for WB samples containing varying amounts of glucose.</li><li><figref idref="f0016">FIG. 12A</figref> depicts the cyclic voltammogram, semi-integral, and semi-derivative of 16 mM ferrocyanide in a 20% hematocrit WB sample.</li><li><figref idref="f0017">FIG. 12B</figref> is an enlargement of the semi-derivative curve of <figref idref="f0016">FIG. 12A</figref>.</li><li><figref idref="f0017 f0018">FIGs. 13A-13C</figref> depict the derivatives of cyclic voltammograms.</li><li><figref idref="f0019">FIG. 14</figref> plots the semi-integral currents recorded as a function of time for the contour profiles of <figref idref="f0016">FIG. 11</figref>.</li><li><figref idref="f0019">FIG. 15</figref> depicts the cyclic voltammograms obtained from an under-filled sensor strip.</li><li><figref idref="f0020">FIGs. 16A</figref> depicts semi-integral plots of cyclic voltammograms obtained from five sensor strips with 1 V/sec scan rates for a sample including 100 mg/dL glucose and 40% hematocrit in WB.</li><li><figref idref="f0020">FIG. 16B</figref> plots the ratio of the forward and reverse scan current values taken at the 0.15 potential as a function of enzyme concentration.</li><li><figref idref="f0021">FIG. 16C</figref> depicts a typical response of the slope of the linear response calibration of the sensor strip as a function of the GO content (%-dry weight).</li><li><figref idref="f0021">FIG. 17</figref> is a schematic representation of a measuring device.</li></ul>

<u>DETAILED DESCRIPTION</u>

0075An electrochemical analytic system determines the concentration of analytes. in a sample, such as the glucose concentration of whole blood. The system includes at least one device that applies gated voltammetric pulse sequences including multiple duty cycles to the sample. Each duty cycle includes a linear, cyclic, or acyclic excitation during which currents (amperage) are measured from a sensor strip while a potential (voltage) applied to the strip is varied linearly with time. Each duty cycle also includes a relaxation that may be provided by an open circuit. The system may compare the resulting current data to determine the concentration of the analyte in the sample, while correcting the results for variations in non-analyte responsive factors. The system also may apply one or more data treatments, including those based on semi-integration, derivatives, and semi-derivatives to analyze the voltammetric data.

0076The gated voltammetric pulse sequences may provide improved accuracy and precision to the analysis, while reducing the completion time of the analysis. Accuracy errors introduced by the hematocrit effect and precision errors introduced by varying cap-gap volume may be reduced through the combination of a diffusion barrier layer with the gated pulse sequences. Errors otherwise resulting from a non-steady-state sensor condition and/or mediator background also may be reduced. The time required for analysis may be reduced by eliminating the need for additional delays and pulses, such as "incubation" delays to provide reagent rehydration, "burn-off" pulses to renew the electrodes, and mediator regeneration pulses to renew the oxidation state of the mediator. The gated pulse sequences also may allow the determination of dynamic current and contour profiles that provide multiple calibration points, under-fill detection, and the ability to apply temperature compensation to the analysis. Because the gated pulse sequences may generate useful data rapidly, the long wait times of conventional coulometry and the inaccuracy of non-steady-state measurements in conventional amperometry may be avoided.

0077<figref idref="f0001">FIGs.1A-1B</figref> depict a sensor strip <b>100,</b> which may be used in the present sensor system. <figref idref="f0001">FIG. 1A</figref> is a perspective representation of an assembled sensor strip <b>100</b> including a sensor base <b>110,</b> at least partially covered by a lid <b>120</b> that includes a vent <b>130,</b> a concave area <b>140,</b> and an input end opening <b>150.</b> A partially-enclosed volume <b>160</b> (the cap-gap) is formed between the base <b>110</b> and the lid <b>120.</b> Other sensor strip designs compatible with the present invention also may be used, such as those described in <patcit id="pcit0032" dnum="US5120420A"><text>U.S. Pat. Nos. 5,120,420 </text></patcit>and <patcit id="pcit0033" dnum="US5798031A"><text>5,798,031</text></patcit>.

0078A liquid sample for analysis may be transferred into the cap-gap <b>160</b> by introducing the liquid to the opening <b>150.</b> The liquid fills the cap-gap <b>160</b> while expelling the previously contained air through the vent <b>130.</b> The cap-gap <b>160</b> may contain a composition (not shown) that assists in retaining the liquid sample in the cap-gap. Examples of such compositions include water-swellable polymers, such as carboxymethyl cellulose and polyethylene glycol; and porous polymer matrices, such as dextran and polyacrylamide.

0079<figref idref="f0001">FIG. 1B</figref> depicts a top-view of the sensor strip <b>100,</b> with the lid <b>120</b> removed. Conductors <b>170</b> and <b>180</b> may run under a dielectric layer <b>190</b> from the opening <b>150</b> to a working electrode <b>175</b> and a counter electrode <b>185,</b> respectively. In one aspect, the working and counter electrodes <b>175, 185</b> may be in substantially the same plane, as depicted in the figure. In another aspect, the electrodes <b>175, 185</b> may be facing, such as described in <patcit id="pcit0034" dnum="US20040054267A" dnum-type="L"><text>U.S. Pat. App. 2004/0054267</text></patcit>.

0080While the working and counter electrodes <b>175, 185</b> may be closer, in one aspect the electrodes <b>175, 185</b> may be separated by greater than 200 or 250 µm. Similarly, while at least one of the electrodes <b>175, 185</b> may be closer, in one aspect at least one electrode may be separated from an upper portion of the lid <b>120</b> by at least 100 µm. In one aspect, the working and counter electrodes <b>175, 185</b> may have surface areas of approximately 1 mm² and 1.2 mm², respectively. The dielectric layer <b>190</b> may partially cover the electrodes <b>175, 185</b> and may be made from any suitable dielectric material, such as an insulating polymer.

0081The counter electrode <b>185</b> balances the potential at the working electrode <b>175</b> of the sensor strip <b>100.</b> In one aspect, this potential may be a reference potential achieved by forming the counter electrode <b>185</b> from a redox pair, such as Ag/AgCl, to provide a combined reference-counter electrode. In another aspect, the potential may be provided to the sensor system by forming the counter electrode <b>185</b> from an inert material, such as carbon, and including a soluble redox species, such as ferricyanide, within the cap-gap <b>160.</b>

0082Alternatively, the sensor strip <b>100</b> may be provided with a third conductor and electrode (not shown) to provide a reference potential to the sensor system. This third electrode may be configured as a true reference electrode or as an inert material that relies on a soluble redox species to provide the reference potential. The third electrode also may allow the measuring device to determine the insertion of a sensor strip and/or if the cap-gap <b>160</b> has filled with sample. Additional conductors and/or electrodes also may be provided on the strip <b>100</b> to provide these and other functions.

0083<figref idref="f0001">FIG. 2</figref> depicts an end-view diagram of the sensor strip depicted in <figref idref="f0001">FIG. 1B</figref> showing the layer structure of the working electrode <b>175</b> and the counter electrode <b>185.</b> The conductors <b>170</b> and <b>180</b> may lie directly on the base <b>110.</b> Surface conductor layers <b>270</b> and <b>280</b> optionally may be deposited on the conductors <b>170</b> and <b>180,</b> respectively. The surface conductor layers <b>270, 280</b> may be made from the same or from different materials.

0084The material or materials used to form the conductors <b>170, 180</b> and the surface conductor layers <b>270, 280</b> may include any electrical conductor. Preferable electrical conductors are non-ionizing, such that the material does not undergo a net oxidation or a net reduction during analysis of the sample. The conductors <b>170, 180</b> preferably include a thin layer of a metal paste or metal, such as gold, silver, platinum, palladium, copper, or tungsten. The surface conductor layers <b>270, 280</b> preferably include carbon, gold, platinum, palladium, or combinations thereof. If a surface conductor layer is not present on a conductor, the conductor is preferably made from a non-ionizing material.

0085The surface conductor material may be deposited on the conductors <b>170, 180</b> by any conventional means compatible with the operation of the sensor strip, including foil deposition, chemical vapor deposition, slurry deposition, metallization, and the like. In the case of slurry deposition, the mixture may be applied as an ink to the conductors <b>170, 180,</b> as described in <patcit id="pcit0035" dnum="US5798031A"><text>U.S. Pat. No. 5,798,031</text></patcit>.

0086The reagent layers <b>275</b> and <b>285</b> may be deposited on the conductors <b>170</b> and <b>180,</b> respectively, and include reagents and optionally a binder. The binder material is preferably a polymeric material that is at least partially water-soluble. Suitable partially water-soluble polymeric materials for use as the binder may include poly(ethylene oxide) (PEO), carboxy methyl cellulose (CMC), polyvinyl alcohol (PVA), hydroxyethylene cellulose (HEC), hydroxypropyl cellulose (HPC), methyl cellulose, ethyl cellulose, ethyl hydroxyethyl cellulose, carboxymethyl ethyl cellulose, polyvinyl pyrrolidone (PVP), polyamino acids such as polylysine, polystyrene sulfonate, gelatin, acrylic acid, methacrylic acid, starch, maleic anhydride, salts thereof, derivatives thereof, and combinations thereof. Among the above binder materials, PEO, PVA, CMC, and PVA are preferred, with CMC and PEO being more preferred at present.

0087In addition to the binder, the reagent layers <b>275</b> and <b>285</b> may include the same or different reagents. In one aspect, the reagents present in the first layer <b>275</b> may be selected for use with the working electrode <b>175,</b> while the reagents present in the second layer <b>285</b> may be selected for use with the counter electrode <b>185.</b> For example, the reagents in the layer <b>285</b> may facilitate the free flow of electrons between the sample and the conductor <b>180.</b> Similarly, the reagents in the layer <b>275</b> may facilitate the reaction of the analyte.

0088The reagent layer <b>275</b> may include an oxidoreductase specific to the analyte that may facilitate the reaction of the analyte while enhancing the specificity of the sensor system to the analyte, especially in complex biological samples. Examples of some specific oxidoreductases and corresponding analytes are given below in Table II. <tables id="tabl0002" num="0002"><table frame="all"><title><b>Table II</b></title><tgroup cols="2"><colspec colnum="1" colname="col1" colwidth="95mm" /><colspec colnum="2" colname="col2" colwidth="33mm" /><thead><row><entry valign="top"><b>Oxidoreductase (reagent layer)</b></entry><entry valign="top"><b>Analyte</b></entry></row></thead><tbody><row><entry>Glucose dehydrogenase</entry><entry>β-glucose</entry></row><row><entry>Glucose oxidase</entry><entry>β-glucose</entry></row><row><entry>Cholesterol esterase; cholesterol oxidase</entry><entry>Cholesterol</entry></row><row><entry>Lipoprotein lipase; glycerol kinase; glycerol-3-phosphate oxidase</entry><entry>Triglycerides</entry></row><row><entry>Lactate oxidase;lactate dehydrogenase; diaphorase</entry><entry>Lactate</entry></row><row><entry>Pyruvate oxidase</entry><entry>Pyruvate</entry></row><row><entry>Alcohol oxidase</entry><entry>Alcohol</entry></row><row><entry>Bilirubin oxidase</entry><entry>Bilirubin</entry></row><row><entry>Uricase</entry><entry>Uric acid</entry></row><row><entry>Glutathione reductase</entry><entry>NAD(P)H</entry></row><row><entry>Carbon monoxide oxidoreductase</entry><entry>Carbon monoxide</entry></row></tbody></tgroup></table></tables> At present, especially preferred oxidoreductases for glucose analysis include glucose oxidase, glucose dehydrogenase, derivatives thereof, or combinations thereof.

0089The reagent layer <b>275</b> also may include a mediator to more effectively communicate the results of the analyte reaction to the surface conductor <b>270</b> and/or the conductor <b>170.</b> Examples of mediators include OTM complexes, coordination complexes, and electro-active organic molecules. Specific examples include ferrocene compounds, ferrocyanide, ferricyanide, coenzymes of substituted or unsubstituted pyrroloquinoline quinones (PQQ), substituted or unsubstituted 3-phenylimino-3H-phenothiazines (PIPT), 3-phenylimino-3H-phenoxazine (PIPO), substituted or unsubstituted benzoquinones, substituted or unsubstituted naphthoquinones, N oxides, nitroso compounds, hydroxylamines, oxines, flavins, phenazines, phenazine derivatives, phenothiazines, indophenols, and indamines. These, and other mediators that may be included in the reagent layer may be found in <patcit id="pcit0036" dnum="US5653863A"><text>U.S. Pat. Nos. 5,653,863</text></patcit>; <patcit id="pcit0037" dnum="US5520786A"><text>5,520,786</text></patcit>; <patcit id="pcit0038" dnum="US4746607A"><text>4,746,607</text></patcit>; <patcit id="pcit0039" dnum="US3791988A"><text>3,791,988</text></patcit>; and in <patcit id="pcit0040" dnum="EP0354441A"><text>EP Pat. Nos. 0 354 441 </text></patcit>and <patcit id="pcit0041" dnum="EP0330517A"><text>0 330 517</text></patcit>.

0090At present, especially preferred mediators for glucose analysis include ferricyanide, ruthenium hexaamine, PIPT, PIPO, or combinations thereof. A review of useful electrochemical mediators for biological redox systems may be found in <nplcit id="ncit0005" npl-type="s"><text>Analytica Clinica Acta. 140 (1982), pages 1-18</text></nplcit>.

0091The reagent layers <b>275, 285</b> may be deposited by any convenient means, such as printing, liquid deposition, or ink-jet deposition. In one aspect, the layers are deposited by printing. With other factors being equal, the angle of the printing blade may inversely affect the thickness of the reagent layers. For example, when the blade is moved at an approximately 82° angle to the base <b>110,</b> the layer may have a thickness of approximately 10 µm. Similarly, when a blade angle of approximately 62° to the base <b>110</b> is used, a thicker 30 µm layer may be produced. Thus, lower blade angles may provide thicker reagent layers. In addition to blade angle, other factors, such as the viscosity of the material being applied as well as the screen-size and emulsion combination, may affect the resulting thickness of the reagent layers <b>275, 285.</b>

0092The working electrode <b>175</b> also may include a diffusion barrier layer (DBL) that is integral to a reagent layer <b>275</b> or that is a distinct layer <b>290,</b> such as depicted in <figref idref="f0001">FIG. 2</figref>. Thus, the DBL may be formed as a combination reagent/DBL on the conductor, as a distinct layer on the conductor, or as a distinct layer on the reagent layer. When the working electrode <b>175</b> includes the distinct DBL <b>290,</b> the reagent layer <b>275</b> may or may not reside on the DBL <b>290.</b> Instead of residing on the DBL <b>290,</b> the reagent layer <b>275</b> may reside on any portion of the sensor strip <b>100</b> that allows the reagent to solubilize in the sample. For example, the reagent layer <b>175</b> may reside on the base <b>110</b> or on the lid <b>120.</b>

0093The DBL provides a porous space having an internal volume where a measurable species may reside. The pores of the DBL may be selected so that the measurable species may diffuse into the DBL, while physically larger sample constituents, such as RB cells, are substantially excluded. Although conventional sensor strips have used various materials to filter RB cells from the surface of the working electrode, a DBL provides an internal volume to contain and isolate a portion of the measurable species from the sample.

0094When the reagent layer <b>275</b> includes a water-soluble binder, any portion of the binder that does not solubilize into the sample prior to the application of an excitation may function as an integral DBL. The average initial thickness of a combination DBL/reagent layer is preferably less than 30 or 23 micrometers (µm) and more preferably less than 16 µm. At present, an especially preferred average initial thickness of a combination DBL/reagent layer is from 1 to 30 µm or from 3 to 12 µm. The desired average initial thickness of a combination DBL/reagent layer may be selected for a specific excitation length on the basis of when the diffusion rate of the measurable species from the DBL to a conductor surface, such as the surface of the conductor <b>170</b> or the surface of the surface conductor <b>270</b> from <figref idref="f0001">FIG. 2</figref>, becomes relatively constant.

0095Furthermore, using too thick of a DBL with a short excitation length may delay when the diffusion rate of the measurable species from the DBL to the conductor surface becomes relatively constant. For example, when duty cycles including sequential 1 second excitations separated by 0.5 second relaxations are applied to a working electrode using a combination DBL/reagent layer having an average initial thickness of 30 µm, a preferred measurable species diffusion rate from the DBL to the conductor surface may not be reached until at least 6 duty cycles have been applied (>∼10 seconds). Conversely, when the same duty cycles are applied to a working electrode using a combination DBL/reagent layer having an average initial thickness of 11 µm, a relatively constant diffusion rate may be reached after the second excitation (∼2.5 seconds). Thus, there is an upper limit for the preferred average initial thickness of the DBL for a given duty cycle. A more in-depth treatment of the correlation between DBL thickness, excitation length, and time to reach a relatively constant diffusion rate may be found in <patcit id="pcit0042" dnum="WO2006042304A"><text>WO 2006/042304, filed October 12, 2005</text></patcit>, entitled "Concentration Determination in a Diffusion Barrier Layer".

0096The distinct DBL <b>290</b> may include any material that provides the desired pore space, while being partially or slowly soluble in the sample. In one aspect, the distinct DBL <b>290</b> may include a reagent binder material lacking reagents. The distinct DBL <b>290</b> may have an average initial thickness of at least 1 µm, preferably, from 5 to 25 µm, and more preferably from 8 to 15 µm.

0097<figref idref="f0002">FIGs. 3A and 3B</figref> depict a working electrode <b>300</b> having a surface conductor 330 and a distinct DBL 305 during the application of long and short read pulses. When a WB sample is applied to the working electrode <b>300,</b> RB cells <b>320</b> cover the DBL <b>305.</b> Analyte present in the sample forms external measurable species <b>310</b> external to the DBL <b>305.</b> A portion of the external measurable species <b>310</b> diffuses into the distinct DBL <b>305</b> to give internal measurable species <b>315.</b>

0098As shown in <figref idref="f0002">FIG. 3A</figref>, when a continuous 10 second read pulse is applied to the working electrode <b>300,</b> both the external and internal measurable species <b>310</b> and <b>315</b> are excited at the surface conductor <b>330</b> by a change in oxidation state. During the long read pulse, the external measurable species <b>310</b> diffuses through the sample region where the RB cells <b>320</b> reside and through the DBL <b>305</b> to the surface conductor <b>330.</b> Diffusion of the external measurable species <b>310</b> through the RB cells <b>320</b> during the read pulse introduces the hematocrit effect to the analysis. Because a substantial portion of the measurable species excited at the surface conductor <b>330</b> originates from outside the DBL <b>320,</b> a long read pulse applied to a sensor strip having a DBL may perform similarly with regards to the hematocrit effect to a short read pulse applied to a strip lacking a DBL.

0099Conversely, <figref idref="f0002">FIG. 3B</figref> represents the situation where a short excitation is applied to the DBL equipped sensor strip <b>300</b> to excite the internal measurable species <b>315,</b> while substantially excluding from excitation the measurable species <b>310</b> external to the DBL <b>305.</b> During the short excitation, the measurable species <b>310</b> either remains external to the DBL <b>305</b> or does not substantially diffuse through the DBL to reach the surface conductor <b>330.</b> In this manner, the short excitation may provide a substantial reduction in the influence of the hematocrit effect on the analysis. By reducing the hematocrit effect, analysis errors (bias) introduced by the sample constituents, including RB cells, may be reduced.

0100Another advantage of selectively analyzing the measurable species internal to the DBL with a short excitation is a reduction of measurement imprecision from sensor strips having varying cap-gap volumes. Variances in the cap-gap volume between sensor strips may lead to imprecision because the electronics in conventional measuring devices apply the same electric potential and perform the same calculations for each analysis. If a read pulse continues past the time when substantially all of the measurable species present in the cap-gap has been analyzed, the analysis no longer represents the concentration of measurable species in the sample, but instead represents the amount of measurable species in the cap-gap; a very different measurement. Thus, a sensor strip having a larger cap-gap volume will show a higher analyte concentration than a sensor strip having a smaller cap-gap volume, independent of the analyte concentration of the sample. By substantially limiting analysis to the measurable species present in the DBL, the imprecision otherwise introduced by manufacturing variability between sensor strips may be reduced.

0101<figref idref="f0003">FIGs. 4A and 4B</figref> are graphs illustrating the improvement in measurement accuracy when a DBL was combined with a short excitation. <figref idref="f0003">FIG. 4A</figref> shows a large inaccuracy represented as the difference between the 16% and 48% calibration lines (the total hematocrit bias span) resulting from a sensor strip lacking a DBL after a 1 second excitation. Conversely, <figref idref="f0003">FIG. 4B</figref> shows a smaller difference between the calibration lines representing a more accurate result when a DBL was combined with a 1 second excitation. The total bias hematocrit span for the DBL combined with a short excitation was nearly two-thirds less than the total bias span without the DBL.

0102As described above and in further detail in <patcit id="pcit0043" dnum="WO2006042304A"><text>WO 2006/042304</text></patcit>, a short read pulse or excitation may provide an improvement in the accuracy and/or precision of an analysis. However, if a single short excitation is used for the analysis, a relatively constant diffusion rate of the measurable species from the DBL to the conductor surface may not be reached during the analysis. This condition also may result in measurement inaccuracy because the concentration of the measurable species within the DBL does not accurately represent that in the sample. Furthermore, the single excitation may not effectively reduce the background signal from the mediator.

0103<figref idref="f0004">FIG. 5</figref> represents an electrochemical analysis <b>500</b> for determining the presence and optionally the concentration of an analyte <b>522</b> in a sample <b>512</b> that may overcome the disadvantages associated with short excitations. In one aspect, the analysis <b>500</b> may reduce bias from mediator background while providing a shorter analysis time with or without a DBL. In a preferred aspect, the analysis <b>500</b> may be completed in less than 3 or less than 1 minute. In a more preferred aspect, the analysis <b>500</b> may be completed in from 2 to 50 or from 4 to 32 seconds.

0104In <b>510,</b> the sample <b>512</b> is introduced to a sensor strip <b>514,</b> such as the sensor strip depicted in <figref idref="f0001">FIGs. 1A-1B and 2</figref>. The reagent layers, such as <b>275</b> and/or <b>285</b> from <figref idref="f0001">FIG. 2</figref>, begin to solubilize into the sample <b>512,</b> thus allowing reaction. At this point in the analysis, an initial time delay, or "incubation period," optionally may be provided for the reagents to react with the sample <b>512.</b> Preferably, the optional time delay may be from 1 to 10 seconds. A more in-depth treatment of initial time delays may be found in <patcit id="pcit0044" dnum="US5620579A"><text>U.S. Pat. Nos. 5,620,579</text></patcit> and <patcit id="pcit0045" dnum="US5653863A"><text>5,653,863</text></patcit>. In one aspect, the analysis <b>500</b> may reduce the need for an incubation period.

0105During the reaction, a portion of the analyte <b>522</b> present in the sample <b>512</b> is chemically or biochemically oxidized or reduced in <b>520,</b> such as by an oxidoreductase. Upon oxidation or reduction, electrons optionally may be transferred between the analyte <b>522</b> and a mediator <b>532</b> in <b>530.</b>

0106In <b>540,</b> a measurable species <b>542,</b> which may be the charged analyte <b>522</b> from <b>520</b> or the charged mediator <b>532</b> from <b>530,</b> is electrochemically excited (oxidized or reduced). For example, when the sample <b>512</b> is whole blood containing glucose oxidized by glucose oxidase in <b>520</b> and transferring an electron to reduce a ferricyanide (III) mediator to ferrocyanide (II) in <b>530,</b> the excitation <b>of 540</b> oxidizes ferrocyanide (II) to ferricyanide (III) at the working electrode. In this manner, an electron is selectively transferred from the glucose analyte to the working electrode of the sensor strip where it may be detected by a measuring device (not shown).

0107The excitation <b>540</b> includes voltammetric scanning where a varying potential or "scan" is applied across the electrodes of the sensor strip <b>514</b> at a substantially fixed rate (V/sec). The scan rate may be slow or fast; however, fast scans are preferred due to the nature of the gated pulse sequences. In one aspect, the rate at which the potential is scanned is at least 2 mV/sec, preferably from 20 to 5000 mV/sec, more preferably from 200 to 2000 mV/sec. At present, an especially preferred scan rate is from 500 to 1500 mV/sec.

0108The duration of the excitation <b>540</b> is at most 4 or 5 seconds, and preferably less than 3, 2, 1.5, or 1 second. In another aspect, the duration of the excitation <b>540</b> is from 0.1 to 3 seconds, from 0.1 to 2 seconds, or from 0.1 to 1.5 seconds. More preferably, the duration of the excitation <b>540</b> is from 0.4 to 1.2 seconds.

0109In <b>550,</b> the currents resulting from the scanning excitation <b>540</b> may be monitored and recorded as a function of the applied potential (voltage). This contrasts with conventional amperometry and coulometry where a constant voltage is applied while the current is measured as a function of time. In one aspect, the current is monitored and recorded during the excitation <b>540.</b> In another aspect, the current is not monitored during the relaxation <b>560</b> or at least during a portion of the relaxation <b>560.</b> In another aspect, the current and the potential at the working electrode may be monitored during at least a portion of the relaxation <b>560,</b> but the values are not used in determining the concentration of the analyte <b>522.</b>

0110In <b>560,</b> the sample undergoes relaxation, where the measuring device may open the circuit through the sensor strip <b>514,</b> thus allowing the system to relax. During the relaxation <b>560,</b> the current applied during the excitation <b>540</b> is substantially reduced by at least one-half, preferably by an order of magnitude, and more preferably to zero. Preferably, a zero current state is provided by an open circuit. In one aspect, the relaxation <b>560</b> is at least 10, 5, 3, 2, 1.5, 1, or 0.5 seconds in duration. In another aspect, the relaxation <b>560</b> is from 0.1 to 3 seconds, from 0.1 to 2 seconds, or from 0.1 to 1.5 seconds in duration. More preferably, the relaxation 360 is from 0.2 to 1.5 seconds in duration and provided by an open circuit.

0111During the relaxation <b>560,</b> the ionizing agent may react with the analyte to generate additional measurable species without the effects of an electric potential. Thus, for a glucose sensor system including glucose oxidase and a ferricyanide mediator as reagents, additional ferrocyanide (reduced mediator) responsive to the analyte concentration of the sample may be produced without interference from an electric potential during the relaxation <b>560.</b>

0112The excitation <b>540,</b> the recordation <b>550,</b> and the relaxation <b>560</b> constitute a single duty cycle. In <b>570,</b> the duty cycle is repeated at least once for a total of at least two duty cycles. In one aspect, the duty cycle is repeated at least twice for a total of at least three duty cycles within 180 seconds, 90 seconds, or less. In another aspect, the pulse sequence of the analysis <b>500</b> includes at least 4, 6, 8, 10, 14, 18, or 22 duty cycles applied during an independently selected 120, 90, 60, 30, 15, 10, or 5 second time period. In another aspect, the duty cycles are applied during a 5 to 60 second time period. In another aspect, from 3 to 18 or from 3 to 10 duty cycles may be applied within 30 seconds or less. In another aspect, from 4 to 8 duty cycles may be applied within 3 to 16 seconds.

0113The repetitive "on" and "off" nature of the duty cycles of the analysis 500 directly contrast with conventional methods where voltage is continuously applied to and current is continuously drawn from a sensor strip for from 5 to 10 seconds during the duration of the read pulse. For these conventional methods, the applied voltage may have a fixed potential or may have a potential that is swept from a positive to a negative potential or from a positive or a negative potential to a zero potential relative to a reference potential. Even at a zero relative potential, these methods continuously draw current from the sensor strip during the read pulse, which permits the electrochemical reaction to continue throughout the read pulse. Thus, in these conventional methods the reaction that produces measurable species responsive to the analyte concentration and the diffusion of the measurable species to the working electrode are both affected by current during the zero potential portion of a conventional read pulse. The analysis <b>500</b> pulse sequences also are markedly different from conventional methods that use a single long duration pulse with multiple measurements, such as those disclosed in <patcit id="pcit0046" dnum="US5243516A"><text>U.S. Pat. No. 5,243,516</text></patcit>, due to the multiple relaxations <b>560.</b>

0114In <b>580,</b> the recorded current and voltage values may be transformed with one or more data treatments. The transformed values may be used to determine the presence and/or concentration of the analyte <b>522</b> in the sample <b>512.</b> The transformed values also may be used to determine other characteristics of the analysis <b>500</b>, including the hematocrit concentration of the sample, multiple calibration sets, under-fill, and the active ionizing agent content of the sensor strip, as outlined below.

0115<figref idref="f0005 f0006 f0007">FIGs. 6A-6F</figref> depict six examples of gated voltammetric pulse sequences that may be used with the method <b>500.</b> In each pulse sequence, multiple duty cycles were applied to the sensor strip after introduction of the sample. The voltammetric excitation portion of each duty cycle may be applied in a linear (<figref idref="f0005">FIG. 6A</figref>), cyclic (<figref idref="f0005">FIG. 6B</figref>), or acyclic manner (<figref idref="f0006 f0007">FIGs. 6C-6F</figref>). In these examples, tilted (linear) or triangular-wave (cyclic or acyclic) excitation pulses were used; however, other wave types compatible with the sensor system and the sample also may be used.

0116<figref idref="f0005">FIG. 6A</figref> depicts multiple tilted excitations where the voltage increased linearly with time to an endpoint. <figref idref="f0005">FIG. 6B</figref> depicts multiple triangular-wave excitations providing cyclic data that includes the complete potential range of the ferricyanide mediator. <figref idref="f0006">FIG. 6C</figref> depicts six duty cycles including six triangular-wave excitations providing acyclic data that starts and ends at substantially the same voltage. Because the last excitation of <figref idref="f0006">FIG. 6C</figref>, a terminal read pulse <b>640,</b> lacks a relaxation, only six duty cycles are shown. <figref idref="f0006">FIG. 6D</figref> depicts seven duty cycles including seven triangular-wave excitations providing acyclic data. The first duty cycle is preceded by an initial incubation period. <figref idref="f0007">FIG. 6E</figref> depicts multiple triangular-wave excitations providing acyclic data that starts and ends at different voltages. <figref idref="f0007">FIG. 6F</figref> depicts multiple triangular-wave excitations resulting in acyclic data that substantially exclude the oxidation and reduction peaks of the ferricyanide/ferrocyanide redox pair.

0117The terminal read pulse <b>640</b> may have the same duration and scan rate as the excitations of the prior duty cycles, as depicted in <figref idref="f0006">FIG. 6C</figref>, or the terminal read pulse <b>640</b> may have a different duration or rate. In one aspect, the terminal read pulse <b>640</b> may be of longer duration and increased voltage in relation to the excitations of the prior duty cycles. The increased voltage may provide the ability to detect a species having a higher oxidation potential, such as a control solution. A more complete discussion regarding terminal read pulses may be found in <patcit id="pcit0047" dnum="US66972905P" dnum-type="L"><text>U.S. Provisional App. No. 60/669,729, filed April 8, 2005</text></patcit>, entitled "Oxidizable Species as an Internal Reference in Control Solutions for Biosensors."

0118Control solutions containing known amounts of glucose may be used to verify that the analysis system is operating properly. Specific formulations for control solutions may be found in <patcit id="pcit0048" dnum="US3920580A"><text>U.S. Pat. Nos. 3,920,580</text></patcit>; <patcit id="pcit0049" dnum="US4572899A"><text>4,572,899</text></patcit>; <patcit id="pcit0050" dnum="US4729959A"><text>4,729,959</text></patcit>; <patcit id="pcit0051" dnum="US5028542A"><text>5,028,542</text></patcit>; <patcit id="pcit0052" dnum="US5605837A"><text>5,605, 837</text></patcit>; and <patcit id="pcit0053" dnum="WO9321928A"><text>PCT publications WO 93/21928</text></patcit>; <patcit id="pcit0054" dnum="WO9513535PCT"><text>WO 95/13535</text></patcit>; and <patcit id="pcit0055" dnum="WO9513536PCT"><text>WO 95/13536</text></patcit>. If the measurement device cannot distinguish between a signal from a control solution versus a sample, control solution readings may be stored as analyte values. Thus, the history of a patent's glucose readings, for example, may be inaccurate regarding diabetic condition.

0119If the control solutions cannot be identified and their responses separated from those of the blood samples by the test meter, glucose readings of the control solutions will be included in the history of the glucose measurements, which could lead to wrong interpretation of a patient's diabetic condition.

0120Each of the duty cycles for the pulse sequences depicted in <figref idref="f0005 f0006 f0007">FIGs. 6A-6F</figref> provide excitation times of shorter duration than the following open circuit relaxation times; however, this is not required. In <figref idref="f0006">FIG. 6C</figref> the duration of the excitations is 0.8 seconds at a rate of 1 V/sec while the duration of each relaxation is about 3.2 seconds. Thus, each duty cycle has a duration of about 4 seconds and the pulse sequence lasts for about 24.8 seconds, including a terminal read pulse to provide a redox intensity (RI) of 0.226 (5.6/24.8). The pulse sequence of <figref idref="f0006">FIG. 6D</figref> provides a lower RI of 0.2 (5.6/28), attributable to the incubation period before the first duty cycle.

0121The higher the RI for a pulse sequence, the less background inaccuracy introduced into the analysis by the mediator. The pulse sequences represented in <figref idref="f0005 f0007">FIGs. 6A-6F</figref> are oxidative pulses, designed to excite (e.g. oxidize) a reduced mediator, which is the measurable species. Thus, the greater the oxidative current applied to the sensor strip in a given time period, the less chance that mediator reduced by pathways other than oxidation of the analyte contributes to the recorded current values. In combination, the multiple excitations of the gated voltammetric pulse sequence may eliminate the need for an initial pulse to renew the oxidation state of the mediator. For ferricyanide, pulse sequences having RI values of at least 0.01, 0.3, 0.6, or 1 are preferred, with RI values of from 0.1 to 0.8, from 0.2 to 0.7, or from 0.4 to 0.6 being more preferred at present.

0122During a linear excitation, such as forward excitation <b>610</b> depicted in <figref idref="f0005">FIG. 6A</figref>, the current at the working electrode is measured while the potential at the working electrode changes linearly with time at a constant rate. The excitation range, such as from -0.5 V to +0.5 V, may cover the reduced and oxidized states of a redox pair so that a transition from a first state to a second state occurs. The current measured at the working electrode may be thought of as having three components: the equilibrium current, the diffusion current, and the surface current. The surface current, which may derive from any species, adsorbed on the electrode, is generally small. The equilibrium and diffusion currents are the primary components represented in the resulting voltammogram.

0123A linear voltammogram (a plot of current verses voltage) may be characterized by a plot that starts at an initial current, reaches a peak current, and decays to a lower diffusion-limited current (DLC) level during the excitation. The initial current is substantially dependent on the applied potential, while the DLC is not. If the scan is slow enough, the DLC may be seen as a plateau region in a voltammogram.

0124The DLC region represents a state where the oxidation or reduction of the measurable species at the conductor surface reaches a maximum rate substantially limited by diffusion. The diffusion may be limited by the rate at which the measurable species travels from the sample to the conductor surface. Alternatively, when the working electrode of the sensor strip includes a DBL, the diffusion may be limited by the rate at which the measurable species travels from the DBL to the conductor surface.

0125DLC values recorded at a relatively constant diffusion rate after rehydration of the reagent layer may minimize inaccuracies that would otherwise be introduced by variations in the rehydration and diffusion rates of the reagents. Thus, once a relatively constant diffusion rate is reached, the recorded DLC values may more accurately correspond to the concentration of the measurable species, and thus the analyte.

0126After completion of the forward excitation <b>610,</b> for a cyclic or acyclic excitation, such as those depicted in <figref idref="f0005">FIGs. 6B</figref> and <figref idref="f0006">6C</figref>, respectively, a reversed potential linear excitation <b>620</b> is applied. The reversed potential linear scan of the excitation <b>620</b> may be applied at substantially the same rate as the forward scan <b>610.</b> Thus, the excitation range is scanned from a first lower value to a higher value and back to a second lower value, where the first and second lower values may or may not be the same for cyclic or acyclic scans, respectively. Cyclic, and in some instances acyclic, excitations may examine the transition of a redox species from a reduced state to an oxidized state (and vice versa) in relation to the applied potential or in relation to the diffusion rate of the redox species to the conductor surface.

0127In relation to a linear excitation, cyclic and acyclic excitations may provide a better representation of the DLC region of the excitation. The advantage of cyclic and acyclic excitations may be especially advantageous for quantifying the DLC from quasi-reversible redox pairs at fast scan rates. Additional information about linear and cyclic scan voltammetry may be found in "<nplcit id="ncit0006" npl-type="s"><text>Electrochemical Methods: Fundamentals and Applications" by A.J. Bard and L.R. Faulkner, 1980</text></nplcit>.

0128<figref idref="f0008">FIG. 7A</figref> presents the data from a 25 mV/sec cyclic excitation of a ferricyanide/ferrocyanide redox pair as a cyclic voltammogram. The voltammogram is characterized by a forward current peak during the forward portion of the scan from -0.3 V to +0.6 V indicating ferrocyanide oxidation and a reverse current peak during the reverse voltage scan from +0.6 V back to -0.3 V indicating ferricyanide reduction. The forward and reverse current peaks center around the formal potential E⁰' of the ferrocyanide/ferricyanide redox pair, when referenced to the counter electrode. In this aspect, the potential of the counter electrode is substantially determined by the reduction potential of ferricyanide, the major redox species present on the counter electrode.

0129While the potentials where the forward and reverse scans begin (the excitation range) may be selected to include the reduced and oxidized states of the redox pair, the excitation range may be reduced to shorten the analysis time. However, the excitation range preferably includes the DLC region for the redox pair. For example, at a scan rate of 25 mV/sec, the concentration of the reduced [Red] and oxidized [Ox] species of the ferrocyanide/ferricyanide reversible redox pair and the resulting electrode potential are described by the Nernst equation as follows. <maths id="math0001" num="(1)"><math display="block"><mi mathvariant="normal">E</mi><mo>=</mo><msup><mi>E</mi><mrow><mn>0</mn><mo>′</mo></mrow></msup><mo>+</mo><mfrac><mi mathvariant="italic">RT</mi><mi mathvariant="italic">nF</mi></mfrac><mi>ln</mi><mfrac><mfenced open="[" close="]"><mi mathvariant="italic">Ox</mi></mfenced><mfenced open="[" close="]"><mrow><mi>Re</mi><mi>d</mi></mrow></mfenced></mfrac><munder accentunder="true"><munder accentunder="true"><mrow><mi>T</mi><mo>=</mo><mn>25</mn><mo>°</mo><mi>C</mi></mrow><mo>_</mo></munder><mo>_</mo></munder><mi mathvariant="normal"></mi><msup><mi>E</mi><mrow><mn>0</mn><mo>′</mo></mrow></msup><mo>+</mo><mfrac><mn>0.059</mn><mi>n</mi></mfrac><mi>log</mi><mfrac><mfenced open="[" close="]"><mi mathvariant="italic">Ox</mi></mfenced><mfenced open="[" close="]"><mrow><mi>Re</mi><mi>d</mi></mrow></mfenced></mfrac><munder accentunder="true"><munder accentunder="true"><mrow><mi mathvariant="normal">n</mi><mo>=</mo><mn>1</mn></mrow><mo>_</mo></munder><mo>_</mo></munder><mi mathvariant="normal"></mi><msup><mi>E</mi><mrow><mn>0</mn><mo>′</mo></mrow></msup><mo>+</mo><mn>0.059</mn><mi>log</mi><mfrac><mfenced open="[" close="]"><mi mathvariant="italic">Ox</mi></mfenced><mfenced open="[" close="]"><mrow><mi>Re</mi><mi>d</mi></mrow></mfenced></mfrac></math><img file="EP1934591B1_D0001.tif" /></maths>

0130In the Nernst equation, R is the gas constant of 8.314 Joul/(mole*K), F is the Faraday constant of 96,5000 Coul./equiv., n is the number of equivalents per mole, and T is the temperature in degrees Kelvin. When the potential at the working electrode is referenced to its own redox potential, the formal potential E⁰' will become substantially zero and the equation collapses to: <maths id="math0002" num="(2)"><math display="block"><mi mathvariant="normal">E</mi><mo>=</mo><mn>0.059</mn><mi>log</mi><mfrac><mfenced open="[" close="]"><mi>Ox</mi></mfenced><mfenced open="[" close="]"><mi>Red</mi></mfenced></mfrac><mo>=</mo><mn>0.059</mn><mi>log</mi><mfrac><mfenced open="[" close="]"><mrow><mi>Fe</mi><msubsup><mfenced><mi>CN</mi></mfenced><mn>6</mn><mrow><mo>−</mo><mn>3</mn></mrow></msubsup></mrow></mfenced><mfenced open="[" close="]"><mrow><mi>Fe</mi><msubsup><mfenced><mi>CN</mi></mfenced><mrow><mn>6</mn><mo>′</mo></mrow><mrow><mo>−</mo><mn>4</mn></mrow></msubsup></mrow></mfenced></mfrac><mo>.</mo></math><img file="EP1934591B1_D0002.tif" /></maths> From equation (2), when the ratio of the oxidized mediator to the reduced mediator changes by 10, the potential at the working electrode changes by about 60 mV. The reverse is also true. Thus, for ferricyanide [Ox] to ferrocyanide [Red] concentration ratios of 10:1, 100:1, 1000:1 and 10,000:1, the potential at the working electrode will be approximately 60, 120, 180, and 240 mV away from the zero potential, respectively.

0131Thus, when the ratio of ferricyanide to ferrocyanide is ∼1000:1, a scan range of -180 mV to +180 mV would provide substantially complete oxidation of the reduced species at the working electrode. At 180 mV, the oxidation rate is limited by how fast the reduced form of the mediator can diffuse to the conductor surface, and from this potential forward, there exists a DLC region. Thus, if the reversing point is set ∼400 mV from the zero potential, ∼200 mV of DLC region may be provided.

0132For reversible systems, it may be preferable to provide an excitation range of from 400 to 600 mV, thus exciting from 200 to 300 mV on each side of the formal potential E⁰' of the redox pair. For quasi-reversible systems, it may be preferable to provide an excitation range of from 600 to 1000 mV, thus exciting from 300 to 500 mV on each side of the formal potential E⁰' of the redox pair.

0133The larger excitation range may be preferred for quasi-reversible systems because the DLC region may be smaller. In addition to redox pairs that are inherently quasi-reversible, fast scan excitation may cause a redox pair that is reversible at slow excitation rates to demonstrate quasi-reversible behavior. Thus, it may be preferable to provide a larger quasi-reversible excitation range for a reversible redox pair at fast excitation rates.

0134Preferably, at least 25, 50, 100, 150, or 300 mV of DLC region is provided by the selected excitation range. In another aspect, the reversing point for a cyclic or acyclic excitation is selected so that from 25 to 400 mV, from 50 to 350 mV, from 100 to 300 mV, or from 175 to 225 mV of DLC region is provided. For reversible systems, the reversing point for a cyclic or acyclic excitation may be selected so that from 180 to 260 mV or from 200 to 240 mV of DLC region is provided. For quasi-reversible systems, the reversing point for a cyclic or acyclic excitation may be selected so that from 180 to 400 mV or from 200 to 260 mV of DLC region is provided.

0135Once the reversing point is selected to provide the desired DLC region, the duration of the reverse scan may be selected for an acyclic scan. As can be seen in <figref idref="f0008">FIG. 7B</figref>, starting the forward scan and terminating the reverse scan at approximately -0.025 mV resulted in an acyclic scan that included more of the forward current peak than the reverse current peak. From the <figref idref="f0008">FIG. 7B</figref> comparison, while the peak currents obtained for the cyclic (a) and acyclic (b) scans differ, the DLC region of the scans were nearly the same, especially with regard to the reverse scan.

0136In another aspect, the reverse excitation may be terminated before the reverse current peak is reached, as depicted in <figref idref="f0009">FIG. 7C</figref>. When the forward excitation was started at a potential sufficiently negative, such as at -0.3 mV in <figref idref="f0009">FIG. 7C</figref>, to the middle of the potential range of the redox pair, such as -0.05 mV in <figref idref="f0009">FIG. 7C</figref>, the forward excitation included the full range of the redox potential of the redox pair. Thus, by terminating the reverse excitation at a potential from 50 to 500 mV, from 150 to 450, or from 300 to 400 mV negative from the reversing point, for example, the reverse current peak may be excluded for the ferricyanide/ferrocyanide redox pair.

0137Similarly, the reverse excitation also may be terminated before the reverse current peak is reached by terminating the excitation when the reverse excitation current deviates in value from the DLC. A change in the reverse excitation current of at least 2%, 5%, 10%, or 25% may be used to indicate the beginning of the reverse excitation current peak.

0138<figref idref="f0009">FIG. 7D</figref> compares a 1 V/sec cyclic voltammogram including the forward and reverse oxidation peaks of the redox pair with a 1 V/sec acyclic voltammogram that excludes the forward and reverse oxidation peaks of a redox pair. The acyclic excitation had starting and ending points of 200 mV and a reversing point of 300 mV. Preferable excitation ranges for acyclic excitations within the DLC region of the ferricyanide/ferrocyanide redox pair, which exclude the forward and reverse oxidation and reduction peaks, are from 10 to 200 mV, more preferably from 50 to 100 mV. While the cyclic voltammogram including the complete scan range significantly decayed after reaching the current peak, the acyclic voltammogram provided a substantially flat current region over the scan range. This current region may be directly correlated with the analyte concentration of the sample.

0139As seen in <figref idref="f0009">FIG. 7D</figref>, the current values recorded for the acyclic excitation are numerically smaller than those of the cyclic excitation, while the background current is lower for the acyclic excitation. This beneficial reduction in background current was unexpectedly obtained without having to initiate the acyclic excitation in the reduction peak portion of the cyclic excitation. Thus, a fast and short acyclic excitation within the DLC region of a redox pair may increase the accuracy of analyte determination due to a reduction in the background current, which may provide an increase in the signal-to-background ratio.

0140Cyclic and acyclic excitations may provide multiple benefits in relation to linear excitations. In one aspect, the portion of the reverse scan from the reversing point to the point where the reverse current peak begins may be a better representation of the true DLC values than the DLC region of the forward scan. The DLC region of the reverse excitation may be a more accurate representation of analyte concentration for quasi-reversible redox systems or at fast excitation rates because the forward excitation may not show a distinct DLC region.

0141Acyclic excitations may have multiple advantages over cyclic excitations including a shorter excitation time and a substantial decrease in the amount of mediator electrochemically converted to the measurable state. Thus, if the mediator is reduced in response to the analyte and electrochemically oxidized during measurement, terminating the reverse excitation before the oxidized mediator is electrochemically reduced decreases the amount of reduced mediator in the sample not responsive to the analyte. Similarly, starting the forward excitation at a potential above that at which the measurable species is reduced also may decrease the amount of reduced mediator in the sample not responsive to the analyte. Both acyclic excitations may allow for a shorter analysis time, a significant benefit for the user.

0142<figref idref="f0010 f0011">FIGs. 8A-8D</figref> show the output dynamic currents plotted as a function of potential from the pulse sequence of <figref idref="f0006">FIG. 6C</figref> using 7 triangular waveform excitations for WB samples containing 40% hematocrit and 0, 50, 100, and 400 mg/dL of glucose. The scan rate was 1 V/sec. Instead of a conventional long duration read pulse resulting in extensive oxidation of the measurable species, each triangular excitation was followed by a relaxation to provide a break in the current profile. The currents from each successive excitation were plotted as a different "rep" line, thus providing rep1 through rep7 for each Figure.

0143The current values from each of the multiple excitations (each rep) in the voltammograms of <figref idref="f0010 f0011">FIGs. 8A-8D</figref> were converted to a single data point and connected to give the contour profiles of <figref idref="f0012 f0013">FIGs. 9A-9C</figref>. For <figref idref="f0012">FIGs. 9A and 9B</figref>, the conversion was accomplished by selecting a current value at the same potential in the DLC region of each successive excitation, such as 300 mV. In <figref idref="f0012">FIG. 9A</figref>, the current values from FIGs. SA-8D were directly plotted as a function of time from the ending of the pulse sequence. In <figref idref="f0012">FIG. 9B</figref>, a semi-integral data treatment was applied to the current values before plotting. For <figref idref="f0013">FIG. 9C</figref>, the multiple excitations were converted to single data points by selecting the peak current value of each rep and using a semi-derivative data treatment. In this manner, the X-axis of the contour profiles are expressed in terms of time, thus mimicking the data obtained from a conventional system at steady-state, where the current change with time is substantially constant. While the recorded voltammogram currents may be treated in multiple ways to extract useful information, semi-integral, semi-derivative, and derivative data treatments are presently preferred.

0144The dynamic current profiles obtained from gated voltammetric pulse sequences are fundamentally different from the current profiles obtained from a conventional analysis using a single read pulse. While currents recorded from a single read pulse derive from a single relaxation/diffusion, each time point in the contour profile of the dynamic currents originates from an excitation after an independent relaxation/diffusion process. Furthermore, as the length of an excitation increases, the correlation between the current and the analyte concentration may decrease, often due to the hematocrit effect. Thus, the accuracy of an analysis using multiple, short excitations may be increased in comparison to an analysis using a longer read pulse having the duration of the multiple excitations combined.

0145The application of these data treatments to glucose analysis is described below. However, a more in-depth discussion of data treatments for transforming electrochemical currents and the related digital implementations may be found in <nplcit id="ncit0007" npl-type="s"><text>Bard, A.J., Faulkner, L.R., "Electrochemical Methods: Fundamentals and Applications," 1980</text></nplcit>; <nplcit id="ncit0008" npl-type="s"><text>Oldham, K.B.; "A Signal-Independent Electroanalytical Method," Anal. Chem. 1972, 44, 196</text></nplcit>; <nplcit id="ncit0009" npl-type="s"><text>Goto, M., Oldham, K.B., "Semi-integral Electroanalysis: Shapes of Neopolarograms," Anal. Chem. 1973, 45, 2043</text></nplcit>; <nplcit id="ncit0010" npl-type="s"><text>Dalrymple-Alford, P., Goto, M., Oldham, K.B., "Peak Shapes in Semi-differential Electroanalysis," Anal. Chem. 1977, 49, 1390</text></nplcit>; <nplcit id="ncit0011" npl-type="s"><text>Oldham, K.B., "Convolution: A General Electrochemical Procedure Implemented by a Universal Algorithm," Anal. Chem. 1986, 58, 2296</text></nplcit>; <nplcit id="ncit0012" npl-type="s"><text>Pedrosa, J.M., Martin, M.T., Ruiz, J.J., Camacho, L., "Application of the Cyclic Semi-Integral Voltammetry and Cyclic Semi-Differential Voltammetry to the Determination of the Reduction Mechanism of a Ni-Porphyrin," J. Electroanal. Chem. 2002, 523, 160</text></nplcit>; <nplcit id="ncit0013" npl-type="s"><text>Klicka, R, "Adsorption in Semi-Differential Voltammetry," J. Electroanal. Chem. 1998, 455, 253</text></nplcit><b>.</b>

0146Semi-integration of a voltammogram may separate the DLC from the hematocrit affected equilibrium current (initial peak) because separate signals may be observed for the hematocrit-affected equilibrium si current and the hematocrit. This is especially true at slow scan rates. The semi-integral of the experimentally obtained voltammetric current <i>i(t)</i> has the following mathematical form: <maths id="math0003" num="(3)"><math display="block"><mfrac><msup><mi>d</mi><mrow><mo>−</mo><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup><mrow><mi>d</mi><msup><mi>t</mi><mrow><mo>−</mo><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mrow></mfrac><mi>i</mi><mfenced><mi>t</mi></mfenced><mo>=</mo><mi>I</mi><mfenced><mi>t</mi></mfenced><mo>=</mo><mfrac><mn>1</mn><msup><mi>π</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mfrac><mstyle displaystyle="true"><mrow><munderover><mo>∫</mo><mn>0</mn><mi>t</mi></munderover><mrow><mfrac><mrow><mi>i</mi><mfenced><mi>u</mi></mfenced></mrow><msup><mfenced><mrow><mi>t</mi><mo>−</mo><mi>u</mi></mrow></mfenced><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mfrac><mi mathvariant="italic">du</mi></mrow></mrow></mstyle></math><img file="EP1934591B1_D0003.tif" /></maths><ul id="ul0002" list-style="none" compact="compact"><li>where <i>i(t)</i> is the time function of the voltammetric current obtained during the scan;</li><li><i>I(t)</i> is a transformation and the semi-integral of <i>i(t)</i>;</li><li><i>u</i> is a transformation parameter; and</li><li><i>d</i>^{<i>-1</i>/<i>2</i>}/<i>dt</i>^{<i>-1</i>/<i>2</i>} is the semi-integration operator.</li></ul>

0147At a sufficiently high oxidation potential, the steady-state semi-integral current is given by: <maths id="math0004" num="(4)"><math display="block"><msub><mi>I</mi><mi mathvariant="italic">lim</mi></msub><mo>=</mo><mi mathvariant="italic">nFA</mi><msup><mi>D</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup><mi>C</mi><mfenced><mrow><mi>coul</mi><mo>/</mo><msup><mi>sec</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mrow></mfenced></math><img file="EP1934591B1_D0004.tif" /></maths> where <i>I_lim</i> is the DLC under the condition of the surface concentration of the oxidizable species being zero. Note that the unit of semi-integral current is coul/sec^{<i>1</i>/<i>2</i>}, which is not the traditional unit for expressing electrical current, which is coul/sec.

0148For simplicity, <i>I_lim</i> is referred to as the semi-integration DLC (SI) with a unit of coul/sec^1/2. This SI current (coul/sec^{<i>1</i>/<i>2</i>}) is only a half-step integration from current (coul/sec). The half-step integration is fundamentally different from coulometry where a full integral is applied to the <i>i-t</i> curve to provide the total charge passing through the electrodes.

0149Although equation (3) gives a theoretical definition of the semi-integral, for digital processing the <i>i-t</i> data may be divided into <i>N</i> equally spaced time intervals between <i>t</i> = <i>0</i> and <i>t</i> = <i>NΔt.</i> One such digital processing algorithm is given by equation (5) where <i>t</i> = <i>kΔt</i> and <i>u</i> = <i>jΔt,</i> and <i>i</i> is determined at the midpoint of each interval. <maths id="math0005" num="(5)"><math display="block"><mi>I</mi><mfenced><mrow><mi>k</mi><mi mathvariant="normal">Δ</mi><mi>t</mi></mrow></mfenced><mo>=</mo><mfrac><mn>1</mn><msup><mi>π</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mfrac><mstyle displaystyle="true"><munderover><mo>∑</mo><mrow><mi>j</mi><mo>=</mo><mn>1</mn></mrow><mrow><mi>j</mi><mo>=</mo><mi>k</mi></mrow></munderover><mfrac><mrow><mi>i</mi><mfenced><mrow><mi>j</mi><mi mathvariant="normal">Δ</mi><mi>t</mi><mo>−</mo><mn>1</mn><mo>/</mo><mn>2</mn><mi mathvariant="normal">Δ</mi><mi>t</mi></mrow></mfenced><mi mathvariant="normal">Δ</mi><msup><mi>t</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mrow><msqrt><mrow><mi>k</mi><mo>−</mo><mi>j</mi><mo>+</mo><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msqrt></mfrac></mstyle></math><img file="EP1934591B1_D0005.tif" /></maths> A preferred algorithm for digital processing is given by: <maths id="math0006" num="(6)"><math display="block"><mi>I</mi><mfenced><mrow><mi>k</mi><mi mathvariant="normal">Δ</mi><mi>t</mi></mrow></mfenced><mo>=</mo><mfrac><mn>1</mn><msup><mi>π</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mfrac><mstyle displaystyle="true"><munderover><mo>∑</mo><mrow><mi>j</mi><mo>=</mo><mn>1</mn></mrow><mrow><mi>j</mi><mo>=</mo><mi>k</mi></mrow></munderover><mfrac><mrow><mi mathvariant="normal">Γ</mi><mfenced><mrow><mi>k</mi><mo>−</mo><mi>j</mi><mo>+</mo><mn>1</mn><mo>/</mo><mn>2</mn></mrow></mfenced></mrow><mrow><mfenced><mrow><mi>k</mi><mo>−</mo><mi>j</mi></mrow></mfenced><mo>!</mo></mrow></mfrac></mstyle><mi mathvariant="normal">Δ</mi><msup><mi>t</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup><mi>i</mi><mfenced><mrow><mi>j</mi><mi mathvariant="normal">Δ</mi><mi>t</mi></mrow></mfenced></math><img file="EP1934591B1_D0006.tif" /></maths> where Γ(x) is the gamma function of x, where Γ(1/2) = π^1/2, Γ(3/2) = 1/2π^1/2, and Γ(5/2) = 3/2*1/2π^1/2, etc.

0150From equation (4) it may be seen that the SI current lacks the time-dependence factor of conventional amperometric methods. Thus, the SI current response may be considered a series of plateau currents, instead of the continuously changing amperometric currents obtained from conventional amperometry. Because the semi-integration allows for quantification of the DLC, a faster scan rate may be used than when peak currents are quantified. Thus, linear, cyclic, or acyclic voltammetry in combination with semi-integration may rapidly generate a DLC in response to glucose concentrations. In this manner, the disadvantages of the long wait times of coulometry and the non-steady-state nature of the current in conventional amperometry may be reduced.

0151Equation (4) also shows that reversible or quasi-reversible redox pairs are preferred for use with semi-integration. This is because the semi-integral from a reversible or quasi-reversible redox pair can exhibit a sharp transition from the reduced state to the oxidized state (and vice versa) and a wide DLC region, thus making the transition easier to determine. Ferricyanide/ferrocyanide and the +3 and +2 states of ruthenium hexaamine are examples of redox pairs demonstrating preferred reversible (slow scan) or quasi-reversible (fast scan) behaviors.

0152Poorly activated electrodes may not provide an acceptable DLC condition even with reversible or quasi-reversible redox pairs. Thus, electrode activation procedures, such as those described in <patcit id="pcit0056" dnum="US5429735A"><text>U.S. Pat. No. 5,429,735</text></patcit>, may be used to achieve the preferred electrode activity.

0153In addition to semi-integrals, semi-derivatives of a voltammogram also may be used to quantify the analyte by measuring the peak of the semi-derivative. The semi-derivative of the experimentally obtained voltammetric current <i>i(t)</i> has the following mathematical forms: <maths id="math0007" num="(7)"><math display="block"><mfrac><msup><mi>d</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup><mrow><mi>d</mi><msup><mi>t</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mrow></mfrac><mi>i</mi><mfenced><mi>t</mi></mfenced></math><img file="EP1934591B1_D0007.tif" /></maths><maths id="math0008" num="(8)"><math display="block"><mfrac><msup><mi>d</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup><mrow><mi>d</mi><msup><mi>t</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mrow></mfrac><mi>i</mi><mfenced><mi>t</mi></mfenced><mo>=</mo><mfrac><mrow><mi mathvariant="italic">dI</mi><mfenced><mi>t</mi></mfenced></mrow><mi mathvariant="italic">dt</mi></mfrac><mo>=</mo><mfrac><mi>d</mi><mi mathvariant="italic">dt</mi></mfrac><mfenced open="[" close="]"><mrow><mfrac><mn>1</mn><msup><mi>π</mi><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mfrac><mstyle displaystyle="true"><mrow><munderover><mo>∫</mo><mn>0</mn><mi>t</mi></munderover><mfrac><mrow><mi>i</mi><mfenced><mi>u</mi></mfenced></mrow><msup><mfenced><mrow><mi>t</mi><mo>−</mo><mi>u</mi></mrow></mfenced><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msup></mfrac></mrow></mstyle><mi mathvariant="italic">du</mi></mrow></mfenced><mo>,</mo><mfenced><mrow><mi mathvariant="italic">coul</mi><mo>/</mo><msup><mi>sec</mi><mrow><mn>3</mn><mo>/</mo><mn>2</mn></mrow></msup></mrow></mfenced></math><img file="EP1934591B1_D0008.tif" /></maths> where <i>I(t)</i> is the semi-integral of the time function <i>i(t).</i> The equations used for the semi-integral, semi-derivative, and the derivative data treatment described below, were implemented with the Electrochemical Workstation software package, version 4.07, revised April 26, 2004, which accompanies the CH Instruments Electrochemical Workstation, model CHI 660A.

0154<figref idref="f0014">FIG. 10A</figref> presents the semi-integral plot of the cyclic voltammogram from <figref idref="f0008">FIG. 7A</figref>. Similarly, <figref idref="f0014">FIG. 10B</figref> presents the semi-integral plot of the acyclic voltammogram from <figref idref="f0009">FIG. 7C</figref>, where the reverse excitation terminated before initiation of the reverse current peak. <figref idref="f0015">FIG. 10C</figref> establishes that when the semi-integral of the cyclic and acyclic excitations of <figref idref="f0008">FIG. 7B</figref> are plotted, the DLC region of the return scan was readily established, permitting an accurate current reading in as little as 50 mV from the reversing point. Furthermore, the peak portion of the semi-integral plot was responsive to the hematocrit content of the sample and the magnitude of the peak may be quantitatively related to the hematocrit level.

0155<figref idref="f0015">FIG. 10D</figref> shows the semi-integrals for the cyclic and 200 to 300 mV acyclic excitations of <figref idref="f0009">FIG. 7D</figref>. The shape of the <i>si</i> voltammogram from the short acyclic excitation differs from the voltammogram of the cyclic excitation because the region of oxidation-reduction transition is missing from the acyclic excitation. By starting the acyclic excitation in the DLC region, the background <i>si</i> current decreased at a faster rate in comparison to that observed for the cyclic voltammogram, thus improving the signal-to-background ratio for the acyclic excitation. Furthermore, the reverse <i>si</i> current from the acyclic excitation shows a plateau more accurately describing the analyte concentration of the sample than the forward <i>si</i> current. In this manner, the acyclic scan of the DLC region provided an increase in accuracy for the analysis when compared to the cyclic excitation.

0156<figref idref="f0016">FIG. 11</figref> shows contour profiles prepared by semi-integrating voltammograms from a seven excitation pulse sequence for WB samples containing 0, 56, 111, 221.75, 455.25, and 712.5 mg/dL of plasma glucose. For each of the glucose concentrations, equilibrium with regards to DBL rehydration was reached at the highest current value in the contour profile for each glucose concentration. Thus, readings <b>1110</b> (highest) and <b>1120</b> (lower) establish that equilibrium was reached regarding DBL rehydration at about four seconds for the 455 mg/dL glucose concentration.

0157Current values recorded at a relatively constant diffusion rate may minimize inaccuracies that would otherwise be introduced by variations in the rehydration and diffusion rates of the reagents. Thus, once a relatively constant diffusion rate is reached, the recorded current values may more accurately correspond to the concentration of the measurable species, and thus the analyte. Furthermore, for <figref idref="f0016">FIG. 11</figref>, the complete analysis may be completed in as few as seven seconds because once the highest current value <b>1110</b> of the contour profile is known, its value may be directly correlated to the analyte concentration. Additional data points may be obtained to reduce background error attributable to the mediator, as previously discussed with regard to RI.

0158Another form of data treatment that may be used to generate a contour profile is semi-derivatization. One implementation of a semi-derivative is to take a full step derivative of the semi-integral, as previously described in relation to equation (8). Unlike the plateau region representing the voltammetric scan in semi-integral plots, semi-derivative plots convert the voltammetric scan data into a peak centered at the transition of the redox pair.

0159<figref idref="f0016">FIG. 12A</figref> depicts the cyclic voltammogram (a), semi-integral (b), and semi-derivative (c) of 16 mM ferrocyanide in a 20% hematocrit WB sample. In this instance, the working electrode of the sensor strip lacked enzyme and oxidized mediator. <figref idref="f0017">FIG. 12B</figref> is an enlargement of the semi-derivative curve of <figref idref="f0016">FIG. 12A</figref> showing the peak height for the forward scan. The value of the forward or reverse scan peak height may be correlated with the analyte concentration of the sample. Furthermore, the semi-derivative data treatment may inherently provide hematocrit compensation for glucose determination, especially for samples including less than 40% hematocrit. A more detailed description of the application of semi-derivative data treatment to glucose analysis may be found in <patcit id="pcit0057" dnum="WO2005114164A"><text>WO 2005/114164, filed May 16, 2005</text></patcit>, entitled "Voltammetric Systems for Assaying Biological Analytes."

0160In addition to semi-integral and semi-derivative data treatments, a derivative data treatment also may be used to generate a contour profile, and thus determine the concentration of the analyte in the sample. <figref idref="f0017 f0018">FIGs. 13A-13C</figref> depict the derivatives of cyclic voltammograms for samples having 20, 40, and 60 % hematocrit. These derivative plots show an initial increase in current as voltage increases, followed by a decrease, and finally a DLC region. The hematocrit effect may be seen in the negative peak located at about 0.1 volts in <figref idref="f0016 f0017">FIGs. 12A-12C</figref>, with higher RB cell concentrations reflected as more negative peak values.

0161While the values of the positive and negative derivative peaks, such as those depicted in the derivative plot of <figref idref="f0018">FIG. 13B</figref>, are concentration dependent, the ratio of the negative peak to the positive peak cancels out the concentration dependence, thus being hematocrit dependent. Because this ratio (HI-DER) is concentration independent and hematocrit dependent, the ratio indicates the percent hematocrit in the sample. Thus, this ratio of the derivative peaks may be used to determine a hematocrit compensation equation for analyte determination. A more detailed description of the application of derivative data treatment to glucose analysis maybe found in <patcit id="pcit0058" dnum="WO2005114164A"><text>WO 2005/114164</text></patcit>.

0162In addition to the ability of the gated pulse sequences to reduce inaccuracy from the hematocrit effect and from the mediator background signal, the combination of the dynamic current profile of each excitation and the resulting contour profiles may be used to provide multiple sets of calibration constants to the sensor system, thus increasing the accuracy of the analysis. Each set of calibration constants obtained may be used to correlate a specific current reading to a specific concentration of measurable species in the sample. Thus, in one aspect, an increase in accuracy may be obtained by averaging the glucose values obtained using multiple sets of calibration constants.

0163Conventional electrochemical sensor systems generally use one set of calibration constants, such as slope and intercept, to convert current readings into a corresponding concentration of the analyte in the sample. However, a single set of calibration constants may result in inaccuracies in the analyte concentration determined from the recorded current values because random noise is included in the measurement.

0164By taking the current value or the transformed current value after data treatment at a fixed time within each duty cycle of a gated voltammetric pulse sequence, multiple sets of calibration constants may be established. <figref idref="f0019">FIG. 14</figref> plots the semi-integral currents recorded at 7.4, 10.65, 13.9, and 17.15 seconds for the contour profiles of <figref idref="f0016">FIG. 11</figref>. Each of these four calibration lines are independent of the other and may be used in at least two ways.

0165First, the multiple sets of calibration constants may be used to determine the number of duty cycles that should be applied during the pulse sequence to obtain the desired accuracy and precision. For example, if the current values obtained from the first three excitations indicate a high glucose concentration, such as >150 or 200 mg/dL, the sensor system may terminate the analysis early, such as after the 4^th excitation depicted in <figref idref="f0016">FIG. 11</figref>. In this manner, the time required for the analysis may be substantially shortened. Such a shortening may be possible because imprecision at high glucose concentrations is typically less than at lower glucose concentrations. Conversely, if the current values obtained from the first three excitations indicate a low glucose concentration, such as ≤150 or 100 mg/dL, the sensor system may extend the analysis to greater than 5 or 7 excitations. Thus, the accuracy and/or precision of the analysis may be increased by including 5 or more duty cycles.

0166Second, the multiple sets of calibration constants may be used to increase the accuracy and/or precision of the analysis by averaging. For example, if the target glucose measurement time is 17.15 seconds, the currents at 10.65, 13.9, and 17.15 seconds can be utilized to calculate the glucose concentrations using the slopes and intercepts from the corresponding calibration lines; therefore, <i>G_10.65</i> = <i>(i_10.65</i> - <i>Int_10.65)</i>/<i>Slope_10.65, G_13.9</i> = (<i>i_13.9 - Int_13.9)</i>/<i>Slope_13.9,</i> and <i>G_17.15</i> = (<i>i_17.15</i> - <i>Int_17.15</i>)/<i>Slope_17.15.</i> Theoretically, these three glucose values should be equivalent, differing only by random variations. Thus, the glucose values G<i>_10.65,</i> G<i>_13.9,</i> and G<i>_17.15</i> may be averaged and the final glucose value of (G<i>_10.65</i> + G<i>_13.9</i> + G<i>_17.15</i>)/3 may be calculated. Averaging the values from the calibration lines may provide a reduction in noise at the rate of 1/√3).

0167An unexpected benefit of gated voltammetric pulse sequences including relatively short excitations and relatively long relaxations, such as that depicted in <figref idref="f0006">FIG. 6C</figref>, is the ability to simplify calibration. While the multiple sets of calibration constants that may be obtained from the dynamic and contour profiles may provide an advantage to the accuracy of the analysis, a pulse sequence such as depicted in <figref idref="f0006">FIG. 6C</figref>, may provide similar accuracy to that obtained using multiple sets of calibration constants from a single set of calibration constants. This effect may be observed in the contour profiles of <figref idref="f0016">FIG. 11</figref> and the resulting calibration lines in <figref idref="f0019">FIG. 14</figref>.

0168This unexpected increase in accuracy may be attributable to the relatively long relaxation times in comparison to the short relaxations. In one aspect, excitation/relaxation time (ERT) ratios from 0.3 to 0.2 are preferred, with ERT ratios from 0.27 to 0.22 being more preferred. For example, a gated voltammetric pulse sequence having an ERT ratio of 0.25 (0.8 seconds/3.2 seconds), such as depicted in <figref idref="f0006">FIG. 6C</figref>, may be preferred to a pulse having an ERT ratio of greater than 0.3, such as the <figref idref="f0005">FIG. 6B</figref> pulse sequence having an ERT ratio of 0.56 (1.4 seconds /2.5 seconds). While not intending to be bound by any particular theory, the relatively long relaxation times may provide a state where the average consumption rate of measurable species during the excitation is balanced by the supply rate of measurable species diffusing into the DBL. In this manner, the multiple sets of calibration constants may collapse into a single set and the conversion of the recorded data into an analyte concentration may be simplified by carrying out the averaging process on the recorded current data before determining the analyte concentration.

0169The dynamic current profiles provided by the multiple duty cycles may be used to determine if the sensor strip has been under-filled with sample, thus allowing the user to add additional sample to the sensor strip. In addition to working and counter electrodes, conventional sensor systems may determine an under-fill condition through the use of a third electrode or electrode pair; however, the third electrode or electrode pair adds complexity and cost to the sensor system.

0170Conventional two electrode systems may be able to recognize that an analysis is "bad," but may not determine if the reason for the failed analysis was caused by under-fill or a defective sensor strip. The ability to determine if under-fill caused the failure of the analysis is beneficial because it may be corrected by adding additional sample to the same sensor strip and repeating the analysis, thus preventing a good strip from being discarded.

0171<figref idref="f0019">FIG. 15</figref> depicts the cyclic voltammograms obtained from an under-filled sensor strip, while <figref idref="f0010">FIG. 8A</figref> depicts a series of seven cyclic voltammograms obtained with a gated voltammetric pulse sequence from a normal-filled sensor strip. In both instances, the scan rate was 1 V/sec. Even though the <figref idref="f0010">FIG. 8A</figref> sample lacked any glucose and the sample used for <figref idref="f0019">FIG. 15</figref> included 400 mg/dL of glucose, the current values obtained from the under-filled strip having the 400 mg/dL glucose concentration were much lower than those from the normal-filled strip having no glucose. Thus, it may be determined by the second duty cycle of the pulse sequence that the currents obtained are lower than a previously selected value and that the sensor strip is under-filled. For example, for the system of <figref idref="f0019">FIG. 15</figref>, initial current values less than 0 signify that the sensor strip is under-filled.

0172In this manner, the gated voltammetric pulse sequences of the present invention allowed for under-fill detection in a two-electrode sensor strip, a function typically requiring a third electrode for conventional sensor systems. Furthermore, the under-fill determination may be made in less than 5 seconds, providing time for the measuring device to signal the user, such as by sending a signal to a light emitting device or a display, to add more sample to the strip.

0173A common problem for the accuracy of strip based analysis methods is that the reagents, especially the enzyme, degrade over time. One of the effects of enzyme degradation is a change in the calibration values, and thus the precision and/or accuracy of the analysis.

0174The dynamic current profiles provided by the multiple duty cycles of the present invention may be used to determine the active ionizing agent content of aged sensor strips, where the ionizing species may have degraded. Knowing the amount of ionizing agent available to react with the analyte may allow for the identification of defective sensor strips and for the correction of the analyte concentration value to provide the desired accuracy and precision to the analysis. In this manner, the accuracy and/or precision of the analysis obtained from sensor strips having varying amounts of active ionizing agent due to manufacturing variability or reagent degradation may be obtained.

0175<figref idref="f0020">FIG. 16A</figref> depicts semi-integral plots of cyclic voltammograms obtained from five sensor strips with 1 V/sec scan rates for a sample including 100 mg/dL glucose and 40% hematocrit in WB. While <figref idref="f0020">FIG. 16A</figref> presents acyclic voltammograms, the method also may be applied to cyclic scans. The ionizing agent used in the reagent layer for the sensor strips was the glucose oxidase (GO) enzyme. Each sensor strip included a dry weight percentage of 1.7, 3.5, 5.3, 7, or 10 percent (weight/weight) GO in relation to the total dry weight of the material forming the regent layer. As seen in the figure, the current values for the forward scans increase in relation to those for the reverse scans as the percentage of ionizing agent increases. Thus, the difference between the forward and reverse scan current values may be used to determine the percent of active ionizing agent present in the reagent layer of the sensor strip.

0176<figref idref="f0020">FIG. 16B</figref> plots the ratio of the forward and reverse scan <i>si</i> current values taken at the 0.15 potential as a function of percent GO. Once the correlation between the forward and reverse current ratios and the percent active GO is determined, the amount of active GO present in a reagent layer may be determined from the current values measured for a strip. The ratio of the forward and reverse scans may be determined before or during the analyte analysis portion of the pulse sequence, thus allowing the user to be notified if the strip is defective.

0177The actual active ionizing agent content of the strip may then be used to alter the calibration slope through a relationship such as shown in <figref idref="f0021">FIG. 16C. FIG. 16C</figref> depicts a typical response of the slope of the linear response calibration of the sensor strip as a function of the GO content (%-dry weight). This plot shows that as the GO content increases, the calibration slope decreases. Thus, if the actual GO content of the reagent layer is calculated from <figref idref="f0020">FIG. 16B</figref>, the affected slope of the GO-based sensor strip may be calculated from the 2^nd order polynomial of <figref idref="f0021">FIG. 16C</figref> using the GO content as the input. The output slope then may be used to correct the glucose concentration value in response to differing amounts of active ionizing agent present in the reagent layer of the sensor strip. In this manner, inaccuracy and/or imprecision that would otherwise result from enzyme degradation may be reduced.

0178<figref idref="f0021">FIG. 17</figref> is a schematic representation of a measuring device <b>1700</b> including contacts <b>1720</b> in electrical communication with electrical circuitry <b>1710</b> and a display <b>1730.</b> In one aspect, the measuring device <b>1700</b> is portable and is adapted to be handheld and to receive a sensor strip, such as the strip <b>100</b> from <figref idref="f0001">FIG. 1A</figref>. In another aspect, the measuring device <b>1700</b> is a handheld measuring device adapted to receive a sensor strip and implement gated voltammetric pulse sequences.

0179The contacts <b>1720</b> are adapted to provide electrical communication with the electrical circuitry <b>1710</b> and the contacts of a sensor strip, such as the contacts <b>170</b> and <b>180</b> of the sensor strip <b>100</b> depicted in <figref idref="f0001">FIG. 1B</figref>. The electrical circuitry <b>1710</b> may include an electric charger <b>1750,</b> a processor <b>1740,</b> and a computer readable storage medium <b>1745.</b> The electrical charger <b>1750</b> may be a potentiostat, signal generator, or the like. Thus, the charger <b>1750</b> may apply a voltage to the contacts <b>1720</b> while recording the resulting current to function as a charger-recorder.

0180The processor <b>1740</b> may be in electrical communication with the charger <b>1750,</b> the computer readable storage medium <b>1745,</b> and the display <b>1730.</b> If the charger is not adapted to record current, the processor <b>1740</b> may be adapted to record the current at the contacts <b>1720.</b>

0181The computer readable storage medium <b>1745</b> may be any storage medium, such as magnetic, optical, semiconductor memory, and the like. The computer readable storage medium <b>1745</b> may be a fixed memory device or a removable memory device, such as a removable memory card. The display <b>1730</b> may be analog or digital, in one aspect a LCD display adapted to displaying a numerical reading.

0182When the contacts of a sensor strip containing a sample are in electrical communication with the contacts <b>1720,</b> the processor <b>1740</b> may direct the charger <b>1750</b> to apply a gated voltammetric pulse sequence to the sample, thus starting the analysis. The processor <b>1740</b> may start the analysis in response to the insertion of a sensor strip, the application of a sample to a previously inserted sensor strip, or in response to a user input, for example.

0183Instructions regarding implementation of the gated voltammetric pulse sequence may be provided by computer readable software code stored in the computer readable storage medium <b>1745.</b> The code may be object code or any other code describing or controlling the functionality described in this application. The data that results from the gated voltammetric pulse sequence may be subjected to one or more data treatments, including the determination of decay rates, K constants, slopes, intercepts, and/or sample temperature in the processor <b>1740</b> and the results, such as a corrected analyte concentration, output to the display <b>1730.</b> As with the instructions regarding the pulse sequence, the data treatment may be implemented by the processor <b>1740</b> from computer readable software code stored in the computer readable storage medium <b>1745.</b>

<u>EXAMPLES</u>

Example 1:

Collection of Voltammetric Data

0184The cyclic voltammogram of <figref idref="f0008">FIG. 7A</figref> was obtained from a CH Electrochemical Work Station by applying a potential between the working and counter electrodes of a sensor strip that varied linearly by 1 V/sec at a scan rate of 0.025 V/sec. The current generated at the working electrode during the application of the potential was recorded and plotted as a function of the applied potential. After the initial 0.8 second excitation, the potentiostat opened the circuit to provide a 3.2 second relaxation. Six additional excitations were applied to the strip using the pulse sequence of <figref idref="f0006">FIG. 6C</figref>. In this manner, seven acyclic voltammograms for glucose concentrations of 0, 50, 100, and 400 mg/dL, as shown in <figref idref="f0010 f0011">FIGs. 8A-8D</figref>, respectively, were obtained.

Example 2:

Establishing Contour Plots for Multiple Data Treatments

0185<figref idref="f0012">FIGs. 9A, 9B</figref>, and <figref idref="f0013">9C</figref> are contour plots from unprocessed voltammetric currents, semi-integral, and semi-derivative data treatments, respectively. In <figref idref="f0012">FIG. 9A</figref>, unprocessed current values at 0.3 V were taken from each forward scan to provide seven data points. The resulting contour plot presents the unprocessed current values as a function of time since each duty cycle included a 0.8 second excitation followed by a 3.2 second relaxation.

0186<figref idref="f0012">FIG. 9B</figref> presents a contour plot of the same voltammetric data converted with semi-integral data processing according to equation (3) and implemented with equations (5) and (6). The implemented semi-integral data processing was that present in the CH Electrochemical Work Station software package, version 4.07, revised April 26, 2004, which accompanies the CH Instruments Electrochemical Workstation, model CHI 660A. After semi-integral processing, the semi-integral currents at 0.3 V were taken from the reverse portion of each scan and plotted as function of time, as previously described with regard to <figref idref="f0012">FIG. 9A</figref>.

0187<figref idref="f0013">FIG. 9C</figref> presents a contour plot of the same voltammetric data converted with semi-derivative data processing according to equation (8). The semi-derivative data processing used was that present in the CH Electrochemical Work Station software package, version 4.07, revised April 26, 2004, which accompanies the CH Instruments Electrochemical Workstation, model CHI 660A. After semi-derivative processing, the peak current value was taken from each scan and plotted as function of time, as previously described with regard to <figref idref="f0012">FIGs. 9A and 9B</figref>. Thus, the Y-axis of <figref idref="f0013">FIG. 9C</figref> has the unit of uCoul/sec^3/2 for the semi-derivative currents.

Example 3:

Constructing Calibration Plots and Determining Analyte Concentration

0188As shown in <figref idref="f0019">FIG. 14</figref>, a calibration plot for the semi-integral data processing method was formed by taking the semi-integral currents from the four different glucose concentration at 8.8, 12.8, 16.8, and 20.8 seconds from <figref idref="f0012">FIG. 9B</figref> and plotting the currents as a function of YSI plasma glucose concentration. Glucose sample concentrations were determined from the calibration plot by plugging in the semi-integral processed current from a sample measurement at a specific time into the slope and intercept of the calibration line.

0189Calibration plots for the unprocessed and semi-derivative processed data were generated similarly. The calibration plots were then used to determine glucose sample concentrations from unprocessed and semi-derivative processed measured current values taken at a specific time.

Example 4:

Determining Analyte Concentration from Multiple Calibration Sets

0190<figref idref="f0003">FIG. 4</figref> depicts at least four calibration lines for times up to 20.8 seconds. For an analysis time of 16.8 seconds, the calibration points at 8.8 and 12.8 seconds were used to calibrate the glucose values. The three glucose values calculated from the 8.8, 12.8 and 16.8 second calibration points were the result of independent oxidations separated by the relaxation time before the 8.8, 12.8 and 16.8 second excitation. While representing the same sample glucose concentration, the concentration values differ by the experimental noise. Thus, by averaging, G = (G_8.8 + G_12.8 + G_16.8)/3, these values, the signal-to-noise ratio of the final glucose concentration value was increased.

0191While various embodiments of the invention have been described, it will be apparent to those of ordinary skill in the art that other embodiments and implementations are possible within the scope of the invention.