Optical tomography of small moving objects using time delay and integration imaging

Description of equivalent WO 2004031809 A2

0001OPTICAL TOMOGRAPHY OF SMALL MOVING OBJECTS USING TIME DELAY AND INTEGRATION IMAGING

0002Related Application

0003This application is a continuation-in-part of co-pending U.S. application number 09/927,151 of Alan C. Nelson, filed August 10, 2001, now patent number 6,522,775 issued July 8, 2003 entitled "APPARATUS AND METHOD FOR IMAGING SMALL OBJECTS IN A FLOW STREAM USING OPTICAL TOMOGRAPHY." Field of the Invention

0004The present invention relates to optical tomographic imaging systems in general, and, more particularly, optical tomography where objects are imaged using optical tomography and time delay and integration.

0005Background of the Invention

0006U.S. application 10/126,026 of Alan Nelson, filed 4/19/2002, entitled "VARIABLE-MOTION OPTICAL TOMOGRAPHY OF SMALL OBJECTS" is incorporated herein by this reference. In Nelson, projection images of shadowgrams are digitally captured by means of conventional CCD or CMOS image detectors. In imaging moving objects, such image sensors require short exposures to "stop motion" in order to reduce motion blur. Short exposures limit the signal to noise ratio that can be attained when imaging moving objects. It is advantageous in optical tomography (OT) to image moving objects such as in a flow stream or entrained in a rigid medium for high throughput analysis. Additionally, in the case of objects entrained in a rigid medium, design of the presentation stage or sample positioner is simpler for constant velocity motion than for rapid stop and go motion. Moreover, in such a system, constant motion produces less vibration than stop and go motion.

0007Generally, time delay and integration (TDI) imaging is based on the concept of accumulating multiple exposures of the same moving object, thereby effectively increasing the integration time available to collect incident light. The object motion must be synchronized with the exposures to ensure a crisp image. Typically, TDI detectors include pixels arranged in rows and columns. An electronic signal is moved from row to row in synchrony with a moving image projected onto the device. The synchronized signal results in an extended integration time without blurring.

0008United States Patent 6,249,341 to Basiji, et al. issued June 19, 2001 entitled "Imaging and Analyzing Parameters of Small Moving Objects Such as Cells," discloses an apparatus where light from an object such as a cell moving through an imaging system is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector. Basiji, et al. define a TDI detector as any pixellated device in which the signal produced in response to radiation directed at the device can be caused to move in a controlled fashion. Basiji, et al. does not address optical tomography, a deficiency overcome by the present invention. Summary of the Invention

0009The present invention provides an apparatus and method for three dimensional (3D) reconstruction of an object of interest moving at a constant velocity. The object of interest is centered. The object of interest is imaged with optical point sources located at multiple projection angles around the object of interest, in cooperation with opposing time delay and integration (TDI) image sensors located at a distance from the objects of interest such that there is no focal plane within the objects of interest during imaging. Each of the TDI sensors has a line transfer rate synchronized to the constant velocity of the objects of interest. In one aspect, the present invention provides a method for three dimensional

0010(3D) reconstruction of objects of interest in a laminar flow stream. Objects of interest are injected into a laminar flow stream such that objects are centered in the laminar flow stream and moving at a constant velocity. The objects of interest are sampled with at least one optical point source located around the laminar flow stream, in cooperation with at least one opposing time delay and integration (TDI) image sensor located opposite the at least one optical point source at a distance from the laminar flow stream such that there is no focal plane within the objects of interest during sampling. Multiple projection angles through objects of interest are sampled as they flow between the at least one optical point source and at least one opposing TDI image sensor. At least one projection image is generated with the TDI image sensor, the line transfer rate of which is synchronized to the flow rate of the objects of interest. In another aspect, the present invention overcomes deficiencies in the prior art by providing a method and system for three dimensional optical tomography using photon point source or parallel beam projections and time delay and integration (TDI) image sensors. More particularly, a system is provided for imaging microscopic objects, including biological cells, in a flow stream or entrained in a rigid medium using optical tomography.

0011It is a motivation of this invention to improve signal to noise ratio of projection images in dynamic optical tomography systems by taking advantage of the ability of TDI image sensors to track moving objects. In another aspect the present invention takes advantage of the ability of TDI image sensors to track objects moving in the direction of charge transfer on the sensor and synchronized to the sensor's line transfer rate. In one embodiment, the present invention provides a method of capturing or digitizing projection images or shadowgrams in an optical tomography instrument with a time delay and integration (TDI) image sensor oriented such that the line transfer vector is parallel to the motion vector of the cells as they are presented to the reconstruction cylinder by either a laminar flow stream or mechanically translated under computer control. Brief Description of the Drawings

0012FIG. 1 schematically shows an example illustration of a Flow Optical Tomography (FOT) system as contemplated by an embodiment of the present invention.

0013FIG. 2 schematically shows an example illustration of a Variable Motion Optical Tomography (VOT) system as contemplated by an embodiment of the present invention. FIG. 3 schematically shows an example illustration of a reconstruction cylinder as contemplated by an embodiment of the present invention.

0014FIG. 4 schematically shows an example illustration of a partial top view of another example reconstruction cylinder.

0015FIG. 5 schematically shows an example illustration of a reconstruction cylinder with point sources and TDI image sensors on different planes.

0016FIG. 6 schematically shows an example of a flow diagram illustrating the operation of a TDI image sensor. Detailed Description of the Preferred Embodiments

0017The invention is described herein with respect to specific examples relating to biological cells, however, it will be understood that these examples are for the purpose of illustrating the principals of the invention, and that the invention is not so limited. In one example, constructing a three dimensional distribution of optical densities within a microscopic volume enables the quantification and the determination of the location of structures, molecules or molecular probes of interest. By using tagged molecular probes, the quantity of probes that attach to specific structures in the microscopic object may be measured. For illustrative purposes, an object such as a biological cell may be labeled with at least one stain or tagged molecular probe, and the measured amount and location of this probe may yield important information about the disease state of the cell, including, but not limited to, various cancers such as lung, breast, prostate, cervical and ovarian cancers.

0018Referring to FIG. 1, there schematically shown is an example illustration of a Flow Optical Tomography (FOT) system as contemplated by an embodiment of the present invention. The invention provides an apparatus and method for imaging small objects in a flow stream or entrained in a rigid medium using optical point source or parallel beam projections, time delay and integration (TDI) image sensors and tomographic image reconstruction. The optical tomography (OT) system includes in one example embodiment, a flow cytometer, including a reconstruction cylinder 12, positioned around capillary tube 2.

0019The system is oriented with reference to a coordinate system 40 having coordinates in the X, Y and Z-directions. In operation, cells 1 are injected into an injection tube 3. The capillary tube may be wider at an injection end 5 and includes a pressure cap 6. A sheath fluid 7 is introduced at tube 8 to create laminar flow within the capillary tube 2. A first source of photons 9a and a first photo detector 10a work together with a pulse height analyzer 11 to operate as a triggering device. Pulse height analyzer 11 operates to provide a first signal 30a for the beginning of a cell, and a second signal 30b for the end of the cell as it moves through the tube. The signals 30a, 30b, 31a and 31b are represented as a light intensity, "I," vs. "TIME" function within pulse height analyzer 11. The pulse height analyzer 11 generates a plurality of signals 14 that are sent to a computer 13 which, after a delay related to the velocity of the moving object and distance between the photo detector and the reconstruction cylinder 12, sends a trigger signal 15 to a reconstruction cylinder 12 to initiate and end data collection for that particular cell. Additionally, a second photon source 9b and a second photo detector 10b may advantageously be positioned at a known distance downstream from the first set such that an interval between the cell setting off a third signal 31a and setting off a fourth signal 31b may advantageously be used to calculate the velocity of the cell and also as a timing signal to synchronize a line transfer rate of a TDI image sensor. The timing signal is transmitted to computer 13 in the plurality of signals 14. The computer 13, which may be any useful personal computer or equivalent, in turn sends synchronization signals 16 to the reconstruction cylinder 12. In this way the movement of the cell along the flow axis 20 is matched by a rate of transfer of charge from one stage of the TDI sensor to the next, as described and shown in more detail below with reference to FIG. 6.

0020Now referring to FIG. 2, there schematically shown is an example illustration of a Variable Motion Optical Tomography (VOT) system as contemplated by an alternate embodiment of the present invention. A VOT system 100 takes advantage of a mechanical positioner to present cells entrained in a rigid medium in a tube one at a time. As compared to the FOT system described with reference to FIG. 1. in the VOT system 100 only one trigger mechanism including a photon source 9 and a photo detector 10 is required since the velocity of the cell can be precisely controlled to synchronize with the TDI sensors in the reconstruction cylinder 12. The trigger here is processed by the pulse height analyzer 11 and the computer 13 and used to start and stop data collection. As indicated by double arrow line the capillary tube in this embodiment is translated along the z-axis through the reconstruction cylinder 12 by a screw drive 18 driven by a computer controlled motor 17. The computer controlled motor 17 receives control information 19 from the computer 13. It will be understood by those skilled in the art having the benefit of this disclosure, that any mechanism capable of translating the capillary tube linearly at a constant velocity can be used in place of the screw drive. Signals from the reconstruction cylinder may be analyzed directly or processed using computerized tomographic image reconstruction techniques to provide two dimensional or three dimensional information about cells. Referring now to FIG. 3, there schematically shown is an example illustration of a reconstruction cylinder 12 as contemplated by an embodiment of the present invention. The reconstruction cylinder 12 comprises a plurality of photon point sources 27 of selectable wavelength that are disposed around and substantially concentric with the capillary tube 2. The plurality of photon point sources 27 operate in conjunction with opposing time delay and integration (TDI) image sensors 25 that are sensitive to selectable portions of the light spectrum, where the TDI image sensors 25 are disposed to receive light from the plurality of photon point sources 27 after it has passed through the capillary tube 2, including any object, such as a cell, moving within the capillary tube 2. Conventional TDI sensors, such as, for example, model number CCD525 and/or model number CCD582 available from Fairchild Imaging, Inc. of Milpitas, CA, USA, feature integrated registers that provide signal information as an available output for processing images according to known principals. Such devices feature fast line transfer rates that can be controlled and synchronized by the user. In operation, during the course of moving through the reconstruction cylinder, the cell 1 passes through at least one photon point source. A feature of the present invention is that a plurality of photon point sources 27 of selectable wavelength are disposed around and concentric with the capillary tube 2. The photon point sources operate in conjunction with the opposing time delay and integration (TDI) image sensors 25 that are sensitive to selectable portions of the light spectrum, thus allowing the acquisition of projections 21 of the light transmitted through the cell 1. In this manner, a set of projection rays can be generated where the projection rays can be described as the straight line connecting the source point to an individual sensing element. For illustrative purposes, one example ray is shown as ray 53. The difference between the number of photons leaving the source point along a particular projection ray and the number of photons received at the particular sensing element is related to the number of photons lost or attenuated due to interactions with the cell and other contents of the capillary tube along the projection ray path.

0021Note that complications may arise from light scatter, photon energy shifts, imperfect geometry and poor collimation, and photons from different sources may arrive at a particular sensing element when multiple source points are energized simultaneously. Construction of the reconstruction cylinder, for example by using the geometry for the pattern of point sources and their opposing detectors as described herein, and by proper timing or multiplexing of activation of the multiple point sources and readout of the sensor arrays, the photon contamination due to these issues can be reduced. Photon contamination can be accounted for by calibration of the system, for example, with no cells present. That is, each light source may be illuminated in turn and its effects on each of the sensors can be measured, thereby providing offset data for use in normalizing the system. An additional calibration step may entail, for example, imaging latex polymer beads or other microspheres or oblate spheroids whose optical properties are known and span the density range of interest for cellular imaging.

0022FIG. 3 is a simplified schematic illustration of a particular geometry and arrangement of point sources and sensors. Only a limited number of point sources and detectors are illustrated for the sake of simplifying the drawing in order to better illustrate the principals of the invention. It will be understood by those skilled in the art having the benefit of this disclosure, that additional point sources and detectors may advantageously be packed together, and that a plurality of such units may advantageously be stacked together at predetermined radial displacements to obtain sufficient projection perspectives, for up to or exceeding 180 projections.

0023Referring now to FIG. 4, there schematically shown is an example illustration of a partial top view of another example reconstruction cylinder 12A. Each section of the reconstruction cylinder 12A includes a TDI image sensor 25. In this example, a plurality of projections 21, here up to three projections 21, can be imaged on each sensor such that 15 projections can be accommodated in each 180 degree wide section of the reconstruction cylinder. In a preferred embodiment two such 180 degree wide sections are used. Those skilled in the art having the benefit of this disclosure will understand that substantially similar results can be achieved with many different geometries or arrangements of point sources and sensors as long as projection images of the cell are obtained from a sufficient number of radial perspectives to enable tomographic reconstruction. Referring to FIG. 5, there shown is a particularly useful design of a reconstruction cylinder 12B as contemplated by an embodiment of this invention. Here, a ring of point sources 27 is place around the capillary tube 2 and a ring of TDI image sensors 25 is placed in a plane below the point sources. While only 4 point sources are shown in the illustration, it will be understood that the ring of TDI image sensors may advantageously comprise a greater number, that being enough to enable tomographic reconstruction of images of moving objects. Further, the TDI image sensors can be below or above the plane of the point sources. The point sources may advantageously generate a cone beam 35. By placing the point sources and TDI image sensors on separate planes, point sources on opposing sides of the cylinder will not physically interfere with other projection cone beams.

0024The curved surface of the capillary tube 2 acts as a cylindrical lens producing a focusing effect that may not be desirable in a projection system. Those skilled in the art having the benefit of this disclosure will appreciate that the bending of photons by the capillary tube 2 can be substantially reduced if the spaces 28 between the point source and the tube and between the tube and the detector surfaces are filled with a material having an index of refraction matching that of the capillary tube. Further, the tube can be optically coupled to the space filling material. Such optical coupling may be accomplished with oil or a gel, for example.

0025Now referring to FIG. 6, there schematically shown is an example of a flow diagram 50 illustrating the operation of a TDI image sensor. Charge corresponding to an image element of the cell is transferred down a column of pixel elements 51 of the TDI sensor in synchrony with the image. The charge transfer occurs sequentially until the accumulated charge from the column is read out at the bottom register of the sensor 26.

0026In one embodiment of the optical tomography system contemplated by the invention, a plurality of TDI sensors 25 are oriented such that each sensor has a direction of line transfer 19 that is the same as that of cell movement 20 along the z- axis. The TDI image sensor line transfer rate is synchronized to the velocity of the cells by timing or clocking signals from the computer 13.

0027The process flow shows a moving cell 1 and its location with respect to a TDI sensor 25 at various times along a time line 34. At time = 0 the cell 1 is just above the TDI sensor 25 and no image is sensed. At time = 1 the cell 1 is partially imaged by the TDI sensor 25. A shadowgram 51 of the cell 1 is imaged one line at a time. Electrical charges 22 corresponding to each image line are transferred to the next line of sensor pixel elements 23 in synchrony with the movement of that image line down the TDI image sensor from time = 0 to time = 5. In this way, electrical charge corresponding to each pixel is accumulated down each column 24 of the TDI detector 25 until it is read out at the bottom register 26 at time = 5. Depending on the number of lines or stages in the TDI image sensor, the signal is boosted (e.g. up to 96 fold with a 96 stage TDI sensor such as a DALSA IL-E2 sensor available from DALSA, Waterloo, Ontario, Canada). TDI image sensors are available with up to 53 KHz line transfer rate. This is equivalent to a frame rate of 53,000 frames/sec. Light Source.

0028Each light source may have the same general characteristics, preferably:

0029• it may approximate a small circular point source,

0030• it may be bright with known spectral content,

0031• the photons emitted from the source may form a beam of a known geometry such as a cone with a small cone angle of 5 to 10 degrees or a pencil beam where all photon rays are parallel. Each source creates data for one projection angle. A plurality of sources arranged along a helix whose axis is the center axis of the capillary tube creates data from multiple projection angles as the cell moves through the module. Depending on the sensor geometry, several point sources could be arranged co-linearly on the same circumference such that the projections do not overlap at the sensor. The desired number of sources is a function of the needed resolution within each planar reconstruction (the x-y plane) or volumetric reconstruction. Further, the wavelength of the sources is selectable either by use of various diode or other lasers or by bandpass filtering of a white or other broadband source, for example a mercury or xenon arc lamp.

0032There are several options that can be employed to create optical source points, such as:

0033• an aperture in front of a laser or other high intensity photon source, • an aperture utilizing surface plasmon focusing of photons on both the entry and exit sides of the pinhole,

0034• an optical fiber with a small cross-section, • a short focal length lens in front of a photon source,

0035• an electron beam that irradiates a point on a phosphor surface (a form of CRT), and

0036• various combinations of the above. The geometry using a diverging beam of light is such that, the closer the point source to the object of interest (the cell), the higher the magnification due to the wider geometric angle that is subtended by an object closer to the source. Magnification in a simple projection system is approximately M=(A+B)/A, where A is the distance between the point source and the object (cell) and B is the distance between the object and the detector. Conversely, if the required resolution is known in advance of the system design, then the geometry can be optimized for that particular resolution. For background, those skilled in the art are directed to Blass, M., editor-in-chief, Handbook of Optics: Fiber Optics and Nonlinear Optics. 2^nd ed., Vol. IV, Mcgraw-Hill, 2001.

0037The invention has been described herein in considerable detail in order to comply with the Patent Statutes and to provide those skilled in the art with the information needed to apply the novel principles of the present invention, and to construct and use such exemplary and specialized components as are required. However, it is to be understood that the invention may be carried out by specifically different equipment, and devices and reconstruction algorithms, and that various modifications, both as to the equipment details and operating procedures, may be accomplished without departing from the true spirit and scope of the present invention.

0038What is claimed is: