EP0342633A3

Transformation du bacillus thuringiensis

Abstract

The present invention describes a method which makes possible for the first time a direct and targeted genetic manipulation of Bacillus thuringiensis as well as the closely related B. cereus with the aid of recombinant DNA technology. Furthermore, the present invention relates to the construction of plasmids and shuttle vectors and the B. thuringiensis strains transformed therewith themselves. Also described is a method for direct cloning, expression and identification of genes in B. thuringiensis and the closely related B. cereus.

EP0342633A3, drawing sheet 1 — Sheet 1 of 1

Term

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Projected expiry passed 17 May 2009, 17.4 years ago.

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Citations
Document	Relation	Office	Category	Cited during
EP0178151A2	Cites	European Patent Office (EPO)	X	Search report
Zeitschrift fuer Allgemeine Mikrobiologie, Band 23, Nr. 9, 1983, Seiten 595-599, Berlin, DE; N. SHIVAROVA et al.: "Microbiological implications of electric field effects. VII. Stimulation of plasmid transformation of B. cereus protoplasts by electric field pulses".	Non-patent	–	–	Search report
Molecular and General Genetics, Band 191, 1983, Seiten 257-262, Berlin, DE; A. KLIER et al.: "Mating between Bacillus thuringiensis and transfer of cloned crystal genes".	Non-patent	–	–	Search report
Archives of Microbiology, Band 139, 1984, Seiten 213-217, Berlin, DE; H.-M. FISCHER et al.: "Introduction of plasmid pC194 into Bacillus thuringiensis by protoplast transformation and plasmid transfer".	Non-patent	–	–	Search report
Annual Meeting of the American Society for Microbiology, 8.-13. Mai 1988, Seite 155, Zusammenfassung H-64, Miami Beach, Florida, US; P.M. MURIANA et al.: "Transformation of plasmid DNA into Lactobacillus species, Bacillus cereus, Enterococcus faecalis and Staphylococcus aureus by electroporation".	Non-patent	–	–	Search report
Journal of Bacteriology, Band 157, Nr. 2, 1984, Seiten 708-711, Baltimore, US; R.E. RUHFEL et al.: "Interspecies transduction of Plasmids among Bacillus anthracis, B. cereus and B. thuringiensis".	Non-patent	–	–	Search report
Proceedings of the National Academy of Sciences, Band 79, Nr. 22, Seiten 6951-6955, Washington, US; J.M. GONZALEZ et al.: "Transfer of Bacillus thuringiensis plasmids coding for delta-endotoxins among strains of B. thuringiensis and B. cereus".	Non-patent	–	–	Search report
CHEMICAL ABSTRACTS, Band 107, Nr. 25, December 1987, Seite 226, Zusammenfassung Nr. 230533h, Columbus, Ohio, US; L.V. KOVTUNENKO et al.: "Plasmid transformation of Bacillus cereus protoplasts", & Mol. Genet. Mikrobiol. Virusol. 1987, Nr. 10, Seiten 27-30.	Non-patent	–	–	Search report
Trends in Biotechnology, Band 6, Nr. 12, Dezember 1988, Seiten 303-309, Cambridge, GB; B.M. CHASSY et al.: "Transformation of Bacteria by electroporation".	Non-patent	–	–	Search report
Fems Microbiology, Letters, Band 44, 1987, Seiten 173-177, Amsterdam, NL; B.M. CHASSY et al.: "Transformation of Lactobacillus casei by electroporation".	Non-patent	–	–	Search report

43 members in 23 offices

This recordMember, by publication datePriority claim13 more offices are in the list below

Priority claims12

Priority claims
Document	Office	Kind	Date
194688	Switzerland	–	1988-05-20
194688	Switzerland	A	1988-05-20
194688	Switzerland	A	1988-05-20
327988	Switzerland	–	1988-09-02
327988	Switzerland	A	1988-09-02
327988	Switzerland	A	1988-09-02
18089	Switzerland	–	1989-01-20
18089	Switzerland	A	1989-01-20
18089	Switzerland	A	1989-01-20
CH19880001946	–	–	–
CH19880003279	–	–	–
CH19890000180	–	–	–

Members43

Family members, oldest first
Document	Office	Kind
FI892359A0	Finland	A0	1989-05-17
DK245689D0	Denmark	D0	1989-05-19
NO892029D0	Norway	D0	1989-05-19
GB8911435D0	United Kingdom	D0	1989-07-05
IE891635L	Ireland	L	1989-11-20
DK245689A	Denmark	A	1989-11-21
FI892359A	Finland	A	1989-11-21
NO892029L	Norway	L	1989-11-21
AU3502089A	Australia	A	1989-11-23
EP0342633A2	European Patent Office (EPO)	A2	1989-11-23
PT90595A	Portugal	A	1989-11-30
IL90334A0	Israel	A0	1989-12-15
IL90334D0	Israel	D0	1989-12-15
GB2219806A	United Kingdom	A	1989-12-20
PL279555A1	Poland	A1	1990-01-22
ZA893764B	South Africa	B	1990-03-28
JPH02119780A	Japan	A	1990-05-07
HUT52157A	Hungary	A	1990-06-28
DD283840A5	German Democratic Republic (until 1990)	A5	1990-10-24
KR900018374A	Republic of Korea	A	1990-12-21
EP0342633A3This record	European Patent Office (EPO)	A3	1991-07-31
NZ229191A	New Zealand	A	1992-04-28
GB2219806B	United Kingdom	B	1993-01-13
AU638208B2	Australia	B2	1993-06-24
AR244805A1	Argentina	A1	1993-11-30
PL164138B1	Poland	B1	1994-06-30
IE62833B1	Ireland	B1	1995-03-08
PT90595B	Portugal	B	1995-05-31
IL90334A	Israel	A	1995-07-31
EP0342633B1	European Patent Office (EPO)	B1	1997-01-08
AT147431T	Austria	T	1997-01-15
ATE147431T1	Austria	T1	1997-01-15
DE58909762D1	Germany	D1	1997-02-20
GR3022245T3	Greece	T3	1997-04-30
ES2099063T3	Spain	T3	1997-05-16
HU213302B	Hungary	B	1997-05-28
CA1339734C	Canada	C	1998-03-17
KR0133924B1	Republic of Korea	B1	1998-04-20
KR19990011604A	Republic of Korea	A	1999-02-18
JP2923651B2	Japan	B2	1999-07-26
KR100225355B1	Republic of Korea	B1	1999-10-15
SK280300B6	Slovakia	B6	1999-11-08
SK304489A3	Slovakia	A3	1999-11-08

55 legal events, as 5 offices reported them to INPADOC

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	Event	Code	Office
2005-05-17	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2002-07-16	Announcement of lapse in spainLapsedFD2A	FD2A	ES
2001-03-02	Notification of lapseLapsedST	ST	FR
2001-03-01	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2001-02-01	Nl: lapsed or anulled due to non-payment of the annual feeLapsedNLV4	NLV4	EP
2001-01-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2001-01-22	Se: european patent has lapsedLapsedEUG	EUG	EP
2001-01-15	Patent ceasedCeasedPL	PL	CH
2000-12-01	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-11-30	Be: lapsedLapsedBERE	BERE	EP
2000-05-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-05-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-05-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-05-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-05-18	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-05-18	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-05-17	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
2000-05-17	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1999-05-11	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-05-06	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-21	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-20	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-20	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-19	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-19	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-16	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-13	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1999-04-12	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1998-01-16	Transfer of patentPC2A	PC2A	ES
1998-01-07	No opposition filedOpposition26N	26N	EP
1997-11-21	No opposition filed within time limitOppositionORIGINAL CODE: 0009261PLBE	PLBE	EP
1997-11-21	Information on the status of an ep patent application or granted ep patentGrantedSTATUS: NO OPPOSITION FILED WITHIN TIME LIMITSTAA	STAA	EP
1997-11-14	Transmission of propertyTP	TP	FR
1997-10-08	Title (correction)BACILLUS THURINGIENSIS TRANSFORMATIONRTI2	RTI2	EP
1997-06-02	Nl: assignments of ep-patentsNLS	NLS	EP
1997-05-16	Definitive protectionFG2A	FG2A	ES
1997-03-31	Validation in greece3022245FG4A	FG4A	GR
1997-03-21	Fr: translation filedET	ET	EP
1997-03-11	It: translation for a ep patent filedITF	ITF	EP
1997-03-11	It: translation for a ep patent filedITF	ITF	EP
1997-02-20	Corresponds to:REF	REF	EP
1997-02-14	Name/firm changedCIBA-GEIGY AG TRANSFER- NOVARTIS AGPFA	PFA	CH
1997-01-15	European patent takes effect as a national patent in ch/liEP	EP	CH
1997-01-08	Designated contracting statesAK	AK	EP
1997-01-08	Corresponds to:REF	REF	EP
1996-11-22	(expected) grantORIGINAL CODE: 0009210GRAA	GRAA	EP
1996-10-03	Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOS IGRAGRAH	GRAH	EP
1996-07-09	Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOS IGRAGRAH	GRAH	EP
1996-02-29	Despatch of communication of intention to grantORIGINAL CODE: EPIDOS AGRAGRAG	GRAG	EP
1993-04-21	First examination report despatched17Q	17Q	EP
1991-07-31	Designated contracting statesAK	AK	EP
1991-06-07	Search report despatchedORIGINAL CODE: 0009013PUAL	PUAL	EP
1989-11-23	Request for examination filed17P	17P	EP
1989-11-23	Designated contracting statesAK	AK	EP
1989-10-07	Public reference made under article 153(3) epc to a published international application that has entered the european phaseORIGINAL CODE: 0009012PUAI	PUAI	EP

Transformation du bacillus thuringiensis

Abstract

Term

Priority claims12

Members43

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Designated states1