CA2162880C

Composite synthetic peptide construct eliciting neutralizingantibodies and cytotoxic t lymphocytes against hiv

Abstract

Peptide constructs comprised of multideterminant T helper peptides from the envelope glycoprotein of HIV previously identified toinduce proliferative responses in four different haplotypes of mice and IL-2 responses in 52-73 % of HIV positive, flu positive patients(cluster peptides), were co-linearly synthesized with the peptide 18 of the V3 loop of HIV-1 gp 160, corresponding to the principalneutralizing determinant of HIV-IIIB and also shown to contain a dominant CTL epitope. Cognate help for peptide 18 antibody was elicitedfollowing a single immunization in all strains of mice which had previously responded to a T cell epitope encompassed by the peptides. Intwo strains of mice, the level of neutralizing antibody achieved was comparable to levels adequate for protection from homologous viralchallenge in chimpanzees. After a single boost, much higher antibody titers for 90 % neutralization in the range of 1:1000 to 1:16,000were achieved. Spleen cells from mice of three distinct MHC haplotypes sharing the Dd class I MHC molecule but with different class IImolecules, immunized with the compound peptides, exhibited enhanced gp160-specific CTL activity.

CA2162880C, drawing sheet 1 — Sheet 1 of 35

Term

Term ended

Expired 13 May 2014, 12.4 years ago.

23 claims: 4 independent, 19 dependent

1
CA 02162880 2001-09-20 - 87 THE EMBODIMENTS OF THE INVENTION IN WHICH AN EXCLUSIVE PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:1. An isolated, purified polypeptide which comprises: a) a first peptide having a multideterminant cluster peptide of a human immunodeficiency virus (HIV) envelope protein which is capable of eliciting a helper T cell response (a cluster peptide);b) a second peptide having an epitope which is capable of eliciting a cytotoxic T cell response;and c) a third peptide having an epitope which is capable of eliciting a high titer neutralizing antibody response to HIV;wherein both of said second and third peptides are derived from the V3 loop of HIV gpl60 envelope protein, and wherein each response is provoked in a plurality of individuals of a mammalian species, and wherein each of said peptides is attached to both of the others in a contiguous manner.
9
An immunogen comprising:a) a first peptide having a multideterminant cluster peptide of a human immunodeficiency virus (HIV) envelope protein which is capable of eliciting a helper T cell response (a cluster peptide);b) a second peptide having an epitope which is capable of eliciting a cytotoxic T cell response;and CA 02162880 2002-01-23 - 89 c) a third peptide having an epitope which is capable of eliciting a high titer neutralizing antibody response to HIV;wherein both of said second and third peptides are derived from the V3 loop of HIV gpl60 envelope protein, and wherein each response is provoked in a plurality of individuals of a mammalian species, and wherein each of said peptides is attached to both of the others in a contiguous manner.
10
A polypeptide selected from the group consisting of:EQMHEDIIS LWDQSLKPCVKR IQRG PGRAFVTIGK, KQIINMWQEVGKAMYAP PISGQIRRIQRG PGRAFVTIGK, RDNWRSELYKYKWKIEPLGVAPTRIQRGPGRAFVTIGK, AVAEGTDRVIEWQGAYRAIRHIPRRIRQGLERRIQRGPGRAFVTIGK, DRVIEWQGAYRAIRHIPRRIRQGLERRIQRGPGRAFVTIGK, DRVIEWQGAYRAIRRIQRGPGRAFVTIGK, AQGAYRAIRHIPRRI RRIQRGPGRAFVTIGK, EQMHEDIISLWDQSLKPCVKRIHIGPGRAFYTTKN, KQ IINMWQ EVGKAMY AP PISGQIRRIHIG PGRAFYTTKN, RDNWRSELYKYKWKIEPLGVAPTRIHIGPGRAFYTTKN, AVAEGTDRVIEWQGAYRAIRHIPRRIRQGLERRIHIGPGRAFYTTKN, DRVIEWQGAYRAIRH I PRRI RQGLERRIHI GPGRAF YTTKN, DRVIEWQGAYRAIRRIHIGPGRAFYTTKN, and AQGAYRAIRHIPRRIRRIHIGPGRAFYTTKN.
14
A method for diagnosing exposure to HIV or for assessing immune function, which comprises:(i) contacting a sample obtained from a subject with a polypeptide of any one of claims 1 to 8 and 10;(ii) determining an immune response to said polypeptide, wherein said immune response is selected from the group consisting of a helper T cell response, a cytotoxic T cell response, and an antibody titer.
17
A method for diagnosing exposure to HIV or for assessing immune function which comprises:(i) contacting a sample obtained from a subject with a polypeptide of claim 10;and (ii) determining an immune response to said polypeptide, wherein said immune response is a helper T cell response, a cytotoxic T cell response, or an antibody titer.

Independent claims5

902 paragraphs in 166 sections, as filed

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PCT/US94/05142

COMPOSITE SYNTHETIC PEPTIDE CONSTRUCT ELICITING

NEUTRALIZING ANTIBODIES AND CYTOTOXIC T LYMPHOCYTES

AGAINST HIV

BACKGROUND OF THE INVENTION 5 Field of the Invention

The invention relates to peptides which comprise covalently linked T helper (Th) epitopes, cytoxic T lymphocyte (CTL) epitopes and epitopes which elicit a neutralizing antibody response (AbN) to an infectious agent, especially a parasitic or viral pathogen. Specific examples focus on application of the invention against Human Immunodeficiency Virus (HIV). The peptides have the further characteristic of evoking all three of these responses in hosts having a broad range

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PCT/US94/05142 of Major Histocompatibility Complex (MHC) types.

The invention is also directed to diagnostic methods for immune function in individuals infected with HIV which utilize the above-described peptides and is further directed to prophylactic or therapeutic vaccines which use the above-described peptides as a component of, or perhaps, as the sole active ingredient in the vaccine composition.

Description of the Related Art 10 Immune responses to HIV antigens elicited during natural infection may be a balance between those regulating viral infection and those antagonistic to the integrity of immune function (1-3) . The determinants which weigh favorably or unfavorably upon this balance are not certain. The initial immune responsiveness of the host seems to influence the course of persistent HIV infection leading to progressive debilitating disease associated with increasing immunologic dysfunction (4-7). The virus may contain structures enabling it to evade the immune system, such as suppressive epitopes or masking carbohydrates, structures inducing clonal restriction (8-10), or structures that elicit deleterious effects such as antibodies which enhance viral infectivity (11-16) or autoreactive antibodies or T cells that contribute to the immunodeficiency (17-20) .

The principal neutralizing determinant (PND) of the HIV envelope is located in the third hypervariable region or V3 loop between cysteine residues 301 and 331 (21-23). Antibodies to this region were initially demonstrated to be type specific in their neutralizing properties and more cross-reactive when examined by peptide binding ELISA (23-26), although more broadly neutralizing antibodies to the V3 loop have also been observed (27). Fortunately for synthetic vaccine development, such antibodies can be raised by

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PCT/US94/05142 immunization with short peptides (21,28,29). The protective efficacy of V3-specific antibodies to homologous cell-free virus challenge has been shown in chimpanzee challenge studies (15,30,31) and most recently protection against viral challenge was achieved by passive transfer into chimpanzees of a mouse-human IgGl chimeric monoclonal antibody specific for the V3 loop (32). Sequence variation in the viral envelope protein in and outside (but affecting) this region results in both neutralization escape mutants, potential CTL escape mutants, and altered cellular tropism (3337) .

A high degree of genetic variability in HIV isolates can be found in infected individuals (38-40) .

HIV isolates from a given individual appear to change over the course of disease. Under immune pressure the virus appears to exhibit differences in phenotypic characteristics such as cytopathicity, replication rates, and cellular tropism during the course of infection. Evidence that the virus may be replicating continuously at low levels during infection and never achieve a state of true latency supports the view that HIV-l produces a chronic active infection and selective mechanisms play an important role in viral persistence (33). Multiple distinct V3 regions encoding the PND of the envelope protein have been detected in isolates of HIV derived from peripheral blood mononuclear cells (PBMC), suggesting that positive selection leads to much diversity of HIV env genes in vivo (40). Nevertheless there is evidence that the PND contains conserved epitopes that are the targets of neutralizing antibodies generated by sequence divergent isolates and that a limited number of peptides from the PND can elicit neutralizing antibodies recognizing multiple isolates, albeit at lower titer, and probably lower affinity (24,25,41).

The criteria for an effective vaccine must be not

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PCT/US94/05142 only that it is safe, i.e., does not contain epitopes that elicit autoimmune or virus enhancing responses, but also that it is capable of eliciting both a cellular immune response and a neutralizing antibody response to all the potential HIV variants prevalent in the infected population. In addition, since the MHC molecule of a given individual will bind and recognize only a subset of potential antigenic determinants recognized by the species as a whole, a synthetic peptide vaccine must also incorporate enough antigenic determinants to elicit recognition by T cells of most HLA types.

Accordingly, in a previous study we constructed six synthetic peptides of 20-33 residues each that correspond to six multideterminant T helper regions of the HIV envelope (42) . Called cluster peptides, these span clusters of distinct but overlapping T helper epitopes recognized by proliferating T cells of three or four haplotypes of mice. These cluster peptides were tested for their ability to stimulate T cell responses in mice immunized with recombinant gp 160 (rgpl60) and in peripheral blood lymphocytes of humans infected with HIV. Mice were also immunized with the cluster peptides to test for the induction of T cells responding to intact gpl60 in vitro. Cluster peptides 3, 4 , and 6 (see sequences in Table I) stimulated T cells from mice of all four MHC haplotypes immunized with rgpl60; and when mice were immunized with the cluster peptide, elicited T cell responses capable of recognizing the whole envelope protein in vitro. Cluster peptide 1, also used in this current study, stimulated proliferation strongly in only one strain of mice, despite the fact that the three other strains recognized

<td rowspan="2"></td><td rowspan="2"> components of this peptide</td><td colspan="4"> the multideterminant region from which</td>

<td> was</td><td> made.</td><td> Thus, the whole had</td><td> less</td>

<td> 35</td><td> activity than</td><td> the</td><td> sum of</td><td colspan="2"> its parts (42) . Cluster</td>

<td></td><td> peptides 1, 3,</td><td> 4,</td><td> and 6</td><td> stimulated significant</td><td> IL-2</td>

<td></td><td> responses in</td><td colspan="2"> peripheral</td><td> blood lymphocytes of</td><td> HIV-</td>

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PCT/US94/05142 positive, influenza positive humans in 64, 73, 52, and

58% of tested cases respectively. It is of interest to note that these high responses were observed despite the fact that the subjects tested were presumably infected with a large number of different substrains of HIV. Cluster peptides 1, 3, and 4 have sequences relatively conserved among North American and European isolates of HIV, and cluster peptide 6 spans the boundary between conserved and variable sequences (43).

A successful peptide vaccine should be capable of eliciting T helper (Th) and cytotoxic T lymphocytes (CTL) responses as well as a neutralizing antibodyresponse in vaccinées of multiple HLA types. Major histocompatibility complex (MHC) class I-restricted CTL appear to play a central role in the recovery from viral infection (81). Although exogenous lymphokines can substitute for T-cell help in the maturation of CTL precursors in vitro, the role of Th in priming CTL in vivo still remains poorly understood, compared to Th-B20 cell collaboration. Although much evidence for a helper requirement in CTL induction exists (82-90) , there is also evidence for CTL responses independent of help (85,91-95). Further, no study to date has shown a necessity for help requiring covalent linkage of a helper antigenic determiant to a CTL determinant, analogous to the linkage of carrier to hapten in cognate help for B cells. This lack of evidence may be due to the fact that the targets of CTL are whole cells, and immunization until recently required whole cells (or tissue grafts) or live viruses. The closest one could come to suggesting determinant linkage was to show that the helper determinant and CTL determinant had to be on the same skin graft to induce rejection (89), but this could not be explored further at the molecular level.

Now that the possibility of peptide immunization for CTL induction has been demonstrated (96-100) , it becomes feasible to address this question using peptides

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2162880 PCT/US94/05142 comprising both helper and CTL determinants. Although recent evidence indicated that a helper site is beneficial (90,101,102), it was not clear if the helper and CTL sites needed to be linked. Indeed, uncoupled helper and CTL epitope peptides were effective in two studies (90,102) and not tested in the other (101), but in the former studies, the mixture of helper and CTL determinant peptides was administered in incomplete Freund's adjuvant emulsion, which sequesters the two peptides in the same microenvironment, or was given at high dose for multiple immunizations.

SUMMARY OF THE INVENTION

The present invention is directed to peptides which provide a broad immune response to an antigen expressed by a pathogen. The antigen is typically one derived from a viral or parasitic pathogen. Our strategy for peptide design was to link each cluster peptide to a short synthetic peptide (peptide 18), previously identified to be an immunodominant site recognized by

CD8 cytotoxic T cells in association with class I molecules, and found within the V3 loop or principal neutralizing determinant region of the HIV-IIIB envelope protein.

Immunization of hosts having a broad range of MHC types, (the H-2 loci in mice, equivalent to the HLA loci of humans) with a peptide of our invention results in an immune response having both humoral and cellular components. On the humoral side, a high titer neutralizing antibody response is observed. With respect to the cellular immune response, both cytotoxic T lymphocytes and T helper cells are elicited.

Proper choice of the epitopes employed evokes such a response that is broadly specific for a number of strains of a pathogen. This is particularly important if there is a great divergence in antigen structure among strains of the target pathogen, for example, as is

CA 02162880 2002-03-15 observed for HIV.

Accordingly, it is one object of the present invention co provide peptides which evoke all of a τ helper response. a cytotoxic T lymphocyte response and s a high titer of neutralising antibody in a plurality of hosts expressing a broad range of MHC types.

The peptides of the present invention are also useful in a diagnostic context. For instance the particular peptides disclosed in the examples can be io* . used in a variety of assay formats to assess the immune function of the T helper cells, cytotoxic T lymphocytes and B cells (both B-cell precursors and mature plasma cells) in individuals infected with BTV. Thus, it is also an object of the present invention to provide diagnostic methods and immune function assays which employ che peptides described herein as reagents.

By virtue of the broad immune response which is elicited in a host immunized with the peptides of the present invention, it is a further object of the present invention to provide vaccines, of either or both of a prophylactic and therapeutic nature, against a parasitic or viral infection, and against HIV-i infection especially.

It is yet a further object of the present invention ζΟ provide a method of immunization of a mammalian host which elicits a broad immune response against a parasitic or viral pathogen, especially the HTV-l virus.

The peptides of the present invention are comprised of a covalent linkage of a peptide having a multidéterminant T helper epitope, a peptide having a cytotoxic T lymphocyte (CTL) epitope, preferably one which elicits CTL that are cross-reactive

PCT/US94/05142

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6 2 8 8 0 with a variety of strains of the target virus, and a peptide having a determinant which elicits a neutralizing antibody (a principal neutralizing determinant (PND)). The epitopes in each case are those which can be shown to be recognized by hosts having a broad range of major histocompatibility complex antigens (MHC). The MHC are also called HLA in humans and are the cell surface proteins which determine, in part whether or not tissue transplants are accepted or rejected by the host. The MHC proteins are involved in presentation of antigens to the immune system in early stages of an immune response. By virtue of the fact that the peptides of the present invention are recognized by a number of different MHC or HLA types, they are expected to be efficacious in a large portion of the host population.

The peptides of the present invention further demonstrate the property of eliciting a high titer of neutralizing antibody against the antigen from which their sequences are derived.

The methods for assessing the immune function of an individual having a viral infection that employ the peptides of the present invention are in vitro tests which measure the response of isolated cells from an individual to incubation of the cells with the peptide. For instance, Th activity can be assessed by measurement of cytokines released specifically in response to incubation of peripheral blood cells from a patient with a peptide of the present invention. A preferred cytokine to be measured is interleukin-2 (IL-2). The method of measurement can be any of the techniques known to the art, for instance, measuring proliferation of an interleukin-2 dependent cell line in supernatants of cultures of the incubated peripheral blood cells. Alternatively, ELISA assay of the IL-2 (or other

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2J 6288 0

V9 cytokine) can be performed. The methods for immunization with the peptide of the present invention can be quite simple, such as intravenous injection of a sterile composition comprised of one or more of the peptides of the present invention and a pharmaceutically acceptable carrier solution or adjuvant. Alternatively, the peptides can be administered bound to the surface of irradiated antigen-presenting cells.

BRIEF DESCRIPTION OF THE DRAWINGS

Figs. 1A, IB. P18 specific antibody response of mice .of four different MHC haplotypes following 15 immunization with cluster peptide 6-18. (a) the primary antibody response 31 days after immunization with 20 nanomoles of peptide (levels at day 21 were lower; see

Results) . (b) an anamestic response to a boost of 10 nanomoles of peptide 37 and 49 weeks after primary immunization. Symbols correspond to individual mice except for + which indicates a prebleed pool.

Figs. 2A-2H. HIV-1 IIIB neutralization profiles of four strains of mice 31 days following a single immunization with peptide PCLUS 6-18.

Figs. 3A-3D. HIV-1 IIIB neutralization profiles of four strains of mice 10 days following a single boost with 10 nanomole of PCLUS 6-18 39-42 weeks post primary immunization. Vn/Vo is plotted versus reciprocal dilution for each numbered serum as in Fig. 2. Animal numbers and symbols represented correspond to those in Figure 2 for the primary response. Note that the abscissa for mouse strain BALB/c is different from the other strains, such that the endpoint dilution for BALB/c is 1: 32,768 whereas for the other strains it is

1:4096.

Figs. 4A, 4B. Competitive binding curves using pl8

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PCT/US94/05142 and rgp 120 as competitor, to assess affinity of antibodies for peptide and whole protein. Solid symbols represent sera with > 90% neutralizing activity at one of the dilutions tested and the open symbols represent sera with < 90% neutralizing activity at the lowest dilution tested. The data are representative of 3-4 experiments.

Figs. 5A, 5B. Fine specificity of neutralizing vs nonneutralizing sera in PCLUS 3-18 (Fig. 5A) and PCLUS

6-18 (Fig. 5B) immunized mice. Neutralizing sera (solid bars) and nonneutralizing sera (open bars) were tested in an ELISA assay on wells coated with P18 substituted peptides. Fifteen pepides with single amino acid substitutions from the HIV-1 IIIB sequence (RIQRGPGRAFVTIGK, SEQ. I.D. NO. 7) toward the HIV-1 RF sequence (**TKGPGRVIYATGQ, SEQ. I.D. NO. 8) were used to coat wells (See Table V). Where the two sequences were identical, an Ala was substituted. Peptides were called 18-1 through 18-15, where the second number indicates the position in the sequence that was substituted. The letter under the number in each graph indicates the amino acid from the RF sequence (or Ala) that was substituted at that position in the corresponding P18 IIIB sequence. An asterisk denotes a deletion. Sera are compared at a dilution of 1:1000.

Figs. 6A, 6B. Binding to P18 variants substituted within the central V3 loop region. Peptide 18 variants substituted at positions 3-10 (as shown in Table V) were used to coat microtiter wells, and sera were tested for binding in an ELISA assay. The letter under the number in each graph indicates the amino acid from the RF sequence (or Ala) that was substituted at that position in the corresponding P18 IIIB sequence. Solid bars represent neutralizing sera and open bars represent nonneutralizing sera. Columns represent the mean absorbance ratio of binding to substituted peptide versus P18 at 405 nm of duplicate readings for

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PCT/US94/05142 individual sera, identified by number, from animals immunized with PCLUS 3-18 (Fig. 6A) or PCLUS 6-18 (Fig. 6B) .

Figs. 7A-7C. Induction of HIV-1 envelope gpl6O-specific 5 CTL activity by immunization with compound peptides in

QS21 adjuvant. 15-12 transfectant target cells were used in each experiment. Fig. 7A, CTL from B10.D2 mice; Fig. 7B CTL from B10.A(5R) mice; Fig. 7C, CTL from B10.SOR) mice. Because the standard error of the mean (SEM) of triplicate wells was consistently less than 8 % of the mean, error bars are omitted for clarity.

Figs. 8A, 8B. The requirement for linkage between helper and CTL determinants for priming of CTL. CTL from B10.D2 mice (Figs. 8A and 8C) or B10.A(5R) mice (Figs.

8B and 8D) were incubated with 15-12 transfectant cells and restimulated in vitro with P18 + interleukin 2 (Figs. 8A and 8B) or with P18-pulsed BALB/c 3T3 fibroblasts (Figs. 8C and 8D) as targets.

Figs. 9A, 9B. Phenotype of the CTL effectors (Fig. 9A), and the helper T cells (Fig. 9B) induced by immunization with the compound peptide constructs. In Fig. 9B, immunization was performed with PCLUS4-18. Figs. 10A-10H show HIV neutralizing activity of PCLUS318MN boosted sera (Figs. 10A-10D) and PCLUS6-18MN boosted sera (Figs. 10E-10H).

Figs. 11A-11H show HIV neutralizing activity of PCLUS618MN boosted sera (Figs. 11A-11H) and PCLUS6.1-18MN boosted sera (Figs. 11E-11H).

Figs. 12A, 12B show CTL response elicited by immunization with P18-MN and PCLUS3-18MN in a variety of adjuvants.

Fig. 13A, 13B show CTL response following two immunizations of peptide P18 or PCLUS 6.1MN in a variety of adjuvants.

DETAILED DESCRIPTION OF THE INVENTION

In an effort to provide help for an enhanced

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PCT/US94/05142 neutralizing antibody response, we have directly linked cluster peptides to peptide 18 (P18), which is contained within the PND. P18 consists of amino acid residues 308-322 of HIV-1 IIIB gpl60s {sequence numbering according to the Los Alamos database (43), which is 7 less than the numbering of Ratner et al. (44) that we used previously (42)) . P18 also contains an immunodominant cytotoxic T cell site (45,46). It is to be understood that the region of gpl60 envelope protein homologous to the P18 region, from other strains of HIV than IIIB, can be employed in a similar manner. For instance, Example III shows results obtained when the P18 region of strain MN is used as the CTL epitope in

The immunogenicity of haptenic peptides has been shown to be increased by linear polymerization or coupling to T helper determinants (47-49). The cluster peptides should provide help in multiple MHC haplotypes. Remarkably high neutralizing titers were .obtained in mice of several MHC types after just a single boost with some of these peptides. We further attempted to examine the fine specificity and affinity of neutralizing antibody directed against peptide 18. This approach can be used in designing peptides for a synthetic peptide vaccine for the immunoprophalaxis and immunotherapy of HIV infection (50).

Some of the materials and methods employed in the Examples described below are used in more than one of the Examples. These materials and methods are described as general materials and methods.

General Materials and Methods

Synthesis of peptides. The cluster peptidepeptide-18 and T helper-peptide-18 constructs were synthesized on an automated peptide synthesizer (No.

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430A; Applied Biosystems™, Foster City,CA) utilizing tboc chemistry (51) according to the sequences shown in Table I (SEQ. I.D. NOS. 1-6). The peptides were cleaved from the resin with HF and initially purified by size exclusion chromatography (P4 Biogel™; BioRad™ Laboratories, Mountain View, CA) . Purification to single peaks was achieved by reverse-phase HPLC on gbondapack reverse-phase C18 analytical and preparative columns (Waters™ Associates, Milford, MA) . Peptide 55-18 was

10< synthesized with an extra Ala at the N-terminus to avoid an N terminal Gin, which would cyclize to form pyroglutamic acid.

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The peptides containing the individual or multideterminant epitopes can be joined together by synthesizing them as a colinear peptide as described above. Alternatively, side-chain carboxylic acid and amino groups can be used to form peptide bonds; connection of the peptides through the side-chains provides an immunogen having a branched structure. In a third embodiment, the peptides can be joined by nonpeptide conjugations. Several methods for conjugating

10. peptides are well-known in the art. One such method is set forth in U.S. Patent 4,886,782.

Mice. H-2 congenic mice on the BIO background and BALB/c mice were purchased from Jackson Laboratories (Bar Harbor, ME) or were bred in our colony at BioCon™

Inc., Rockville, MD. Mice used in this study were 8-20 weeks old.

ELISA. Wells of round bottom flexible PVC microtiter plates (#3912 Falcon Labware, Oxnard, CA) were coated overnight at 4° C with 100 μΐ of 10 μΜ Peptide 18, substituted peptide 18, cluster peptide 3, cluster peptide 6, 0.2μ9/πι1 recombinant gp 120 (ABT, Advanced

Biotechnologies, MA) or 2 μΜ sperm whale myoglobin in 0.1M carbonate buffer pH 9.6. Recombinant gpl20 plates were coated with 100 μΐ of a 0.2 μg/ml afftinity25 purified rgpl20 (ABT) in carbonate buffer. The plates were blocked with 1% BSA in phosphate buffered saline (PBS) for l-1.5h. at 4° C and washed with PBS containing 0.05% Tween 20 and 1% BSA (PBSTB). Next, 100μ1 of test serum was added to duplicate wells and incubated for l3 0 1.5h. at 4° C. Test sera were assayed at 10 fold dilutions in PBSTB ranging from 1:100-1:10,000. The wells were then washed with 200 μΐ PBSTB 10 times using an automatic plate washer (BioRad™ Model 1550) and incubated for lh at 4° C with 100 μΐ alkaline phosphatase-conjugated goat anti-mouse IgG (Promega™,

Madison, WI) diluted 1:7500 in PBSTB. After 10 washes, bound antibodies were detected by the addition of 100μ1

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PCT/US94/05142 of ltng/ml paranitrophenyl phosphate as substrate. The optical density at 4 05nm was read with an ELISA reader. Specific absorbance was determined as the mean optical density at 405nm on the the relevant antigen coated wells minus the optical density 405nm on nonrelevant sperm whale myoglobin coated wells. IgM specific for peptide 18 was detected using goat anti -mouse μ chain specific antibody (Sigma Chemical Co., St Louis, MO) followed by alkaline phosphatase conjugated anti goat.

« Total IgG and isotypes IgGl and IgG2a were detected using biotin conjugated rat anti mouse monoclonals LOMG1-13 for IgGl and LO-MG2a-3 for IgG2a (BioSource™ International, Westlake Village, CA) . Following the addition of strepavidin alkaline phosphatase (Sigma

Immunochemicals, St. Louis, MO) substrate was added and plates read on the ELISA reader.

The invention is illustrated in detail by the Examples presented below. The Examples are presented as illustrations of preferred embodiments of the invention and are not meant to be limiting of the invention in any manner.

EXAMPLE I; IMMUNIZATION OF MICE TO PRODUCE HIGH TITER

NEUTRALIZING ANTIBODY AGAINST HIV-1

The peptides of the present invention can be used to 25 produce a high titer of AbN against a target antigen by immunization of a mammalian host with the peptide. In this Example, peptides derived from the sequence of the HIV-1 envelope glycoprotein gp 120 are used to elicit high titers of AbN in mice.

Immunizations. Mice were immunized intraperitoneally with 20 nanomoles of each peptide emulsified 1:1 in Freund's Complete Adjuvant (CFA). At days 21 and 31 post immunization blood was drawn by retroorbital bleed, allowed to clot and the serum removed and frozen at -20°

C. Because antibody levels were found to still be rising between day 21 and day 31 after primary immunzation, the results from 31-day sera are reported.

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Selected groups of animals were boosted 36-52 weeks post primary immunization with 10 nanomoles of peptide in CFA intraperitoneally and bled 10-11 days later, when the secondary response was generally found to be optimal.

Neutralization assays of HIV-1. The quantitative infectivity microassay using CEM-SS cells was performed as described previously (52). Briefly, serial two-fold dilutions of 50 μΐ heat inactivated (56° C , 30 min.) test serum mixed with 50 μΐ of culture supernatant containing 200 syncytium forming units (SFU) of HIV-1IIIB, or HIV-1 MN strain grown optimally from logarithmic cultures of H9 cells and previously cryopreserved and titered were incubated for 30 min at room temperature. The mixtures were added to duplicate wells containing 5 χ 104 DEAE-dextran treated CEM-SS cells for 1 h at 37° C, after which the virus-antibody mixtures were removed and replaced with medium, and the cells cultured only in complete medium for 5 days (for

HIV-1 IIIB) or 4 days (for HIV-1 MN, determined to be optimal for each virus strain) at 37° C in 5% C02. Units of infectious virus were quantitated by subsequent syncytia formation of infected cells under an inverted microscope. The reciprocal geometric mean neutralization titer was expressed as the serum dilution capable of inhibiting HIV-1 foci by greater than 90% of control CEM-SS/HIV-1 infected cells infected with the indicated strain of HIV-1 (i.e. Vn/Vo < 0.1). The assay measures neutralization of cell-free virus in the first incubation, not inhibition of syncytium formation, which is only the readout for enumerating infectious virus. No cytostatic or toxic properties of the serum alone on the CEM-SS cells were observed at the hightest concentration tested. Also, the heat inactivation at 56°

C for 30 min has been shown to eliminate the nonspecific neutralizing activity of mouse sera (53).

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Determination of direct binding by immunofluorescence. To assess binding of peptideinduced antibodies to native gp 120, serial ten-fold dilutions of selected neutralizing and nonneutralizing sera were tested for binding to viral gp 120 expressed on the surface of HIV-1 IIIB productively infected cells in a live cell immunofluorescence assay (IFA) (52).

Competition ELISA binding curves. The binding tests were performed by mixing in sterile polypropylene tubes 125 μΐ of different dilutions of pl8 (0-20 μΜ) or rgpl20 (0-160 nM) in phosphate buffered saline pH 7.2, 1% ovalbumin, 0.05% Tween 20 with 125μ1 of a constant dilution of antisera determined to be in the linear range of absorbance versus antibody dilution in the same buffer. After overnight incubation at 4o C with gentle shaking, a volume of 100 μΐ was added to duplicate wells of a microtiter plate coated with the respective competing antigen pl8 or gpl20, incubated at 4o C for 20 min. and a standard ELISA assay performed. Binding curves were generated using a four parameter logistic function of log serum dilution versus absorbance using a commercial software program (Biometalics™ Inc., Princeton, NJ ) and an estimated dissociation constant (Kj value) for individual sera was determined.

Binding to P18 substituted peptides. The specificity of neutralizing and nonneutralizing sera were tested in a standard ELISA assay for binding to fifteen substituted pl8 peptides (37) coated onto plastic microtiter wells.

The synthetic multideterminant peptides (Table I), called cluster peptides (abbreviated PCLUS in the names of specific constructs), each constitute clusters of overlapping, but distinct, shorter T helper determinants identified in previous studies (42,54,55). Three cluster peptides, PCLUS 3, 4, and 6 used in this study were chosen because they fulfilled the criteria of eliciting proliferative responses in four independent

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MHC haplotypes of mice that differ in both an I-A and ΙΕ molecule, and also in humans of multiple HLA types (42,56,57). PCLUS 1 was strongly recognized by only one strain of mice, B10.BR, yet stimulated IL-2 production in 23 of 36 HIV seropositive, flu-positive donors. Peptide HP53 (residues 827-841 in the Los Alamos database (43) numbering, which is 7 less than that of Ratner (44) used previously (42,54,55)) and peptide HP55 (residues 834-848) have previously been identified as T helper epitopes of the HIV IIIB envelope sequence in mice of the AkEkand AbEb haplotypes and the AkEk, AbEb, AdEd, and ASES haplotypes respectively, and are contained within the longer PCLUS peptides. (Eb and Es are used here to indicate the expressed E0bEa and Ε0ΈΟ molecules, respectively. Although the nonpolymorphic Eo is not expressed in pure H-2b and H-2s haplotypes, we used recombinant strains that express Ea) . Peptide HP53 (also referred to as env TH 4.1) was also previously shown to elicit IL-2 production in peripheral blood lymphocytes of asymptomatic HIV-seropositive human patients (57) . Peptide 18 (residues 308-322) is a B cell epitope located within the hypervariable V3 loop region of the HIV-1 IIIB envelope known as the principal neutralizing determinant (PND), and is the major immunodominant cytotoxic T cell epitope in mice (45), as well as being recognized by human CTL (46).

Synthetic peptide vaccine constructs were prepared by synthesizing peptide 18 at the carboxy terminus of the cluster peptide. Immunization of mice with 20 nanomoles of these constructs produced enhanced peptide 18 specific antibodies, whereas no peptide 18 specific antibody was detected in mice immunized with peptide 18 alone (Table II). The orientation of T helper cell and B cell epitopes proved crucial for the immunogenicity of the construct since although cluster peptide 3-18 elicited an antibody response in all four strains tested, the reverse polarity construct, P18-cluster

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PCT/US94/05142 peptide 3 in which the helper site was C-terminal to P18, elicited an antibody response to peptide 18 in only one strain, B10.HTT, and at a significantly lower level.

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Tnble Π

HIV HID Peptide 18 Specific Antibody Response and Neutralizing Activity in Pour Strains of Mice 31 days Following a Single Immunization with 20 nanomole T Helper- P18 Peptide _

s ci co +Ϊ υ

u c

cc «C

O «ft <

Q £

Q

CJ

CC oc

Cc cc tRccIprocnl Geometric Mcnn HIV IIIIÎ Ncntrnllzlng Antibody Titer expressed as the scrum dilution capable of Inhibiting HIV MB specific foci by greater than 90% of control CEM-SS / HIV MB infected cells. (No. of mice with positive neutralizing titers / total tested.) t NT Not tested 1 B 10.D2 and / or Β 10.A mouse strains used.

§ For PCLUS 3-18 and PCLUS 6-18, two different experiments are shown.

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In most cases the T helper peptide component of our constructs provided help for peptide 18-specific antibody in the strains in which these T cell epitopes elicited proliferative responses in previous studies (54,58). In the case of peptide 53-18 and 55-18, the

AbEb andAkEk haplotypes responded to peptide 53-18 and the ASES, AdEd, and AbEb haplotypes responded to peptide 55-18 (Table II). In addition, AsEsmice responded to peptide 53-18, but AkEk mice failed to make anti-P18 antibodies to peptide 55-18 even though they were previously shown to proliferate in response to P55 (54) . PCLUS 1-18, which encompasses T helper cell epitopes recognized by all four strains of mice used in this study, was able to elicit a strong antibody response in. only one strain,

B10.BR, and a marginal one in two other strains, B10.D2 and B10.HTT. This was not surprising since in our previous study (42) we showed that cluster peptide 1 was not simply the sum of its parts, but failed to stimulate proliferation in some strains in which a smaller component of the cluster peptide did. The larger peptide may fold back upon itself and hinder interaction with the MHC or T cell receptor dr may undergo different processing which would destroy a component epitope. PCLUS 3-18, 4-18, and 6-18 elicited strong peptide 18 antibody responses in all th© strains of mice tested in at least one experiment, as measured by ELISA. These results show that by linking a B cell epitope to the carboxy terminus of a cluster of immunodominant T cell epitopes, in most cases the processing of individual epitopes is patent and the T cells elicited are capable of providing help to the B cell for production of specific antibody. Since a single immunization with these cluster peptide-peptide-18 constructs could induce high levels of P18-specific antibodies, we tested to see if a boost, which might represent a viral challenge, would produce a characteristic anamnestic response resulting in enhanced peptide 18 specific antibody

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PCT/US94/05142 (Figure 1). After the primary immunization, the response to which is slower than a secondary immunization, sera were obtained on days 21 and 31, but the antibody levels were still rising on day 31.

Therefore, we show in panel A individual mean peptide specific absorbance readings for animals immunized with 20 nanomoles of PCLUS 6-18 and bled 31 days post immunization and compare in panel B individual absorbance readings for animals boosted with 10 nanomole of PCLUS 6-18, 37 and 49 weeks post primary immunization and bled 11 days later, when the secondary response usually peaks. The increase in antibody response observed after boosting was between 2.5 to 12 fold.

To assess usefulness of these synthetic peptide constructs in a vaccine, it was necessary to determine if the antibody elicited to peptide 18 following a single immunization with these constructs was capable of neutralizing the virus in vitro. Neutralizing activity, expressed as the reciprocal geometric mean titer capable of inhibiting cell-free infectious units of HIV IIIB by greater than 90% compared to control CEMSS/HIV IIIB infected cells was elicited in_peptide 5318, 55-18, and PCLUS 1-18 in the strains which responded by a specific antibody response. However antibody was neutralizing in only one of five animals immunized with 53-18 and only two of five immunized with 55-18 and PCLUS 1-18 (Table II). This occurred despite equal or higher levels of peptide 18 specific antibodies by ELISA in other animals within the group. The finding that total peptide 18-specific antibody levels did not correlate with neutralization was extended by an interstrain comparison in animals immunized with PCLUS 4-18. Although all strains responded with significant levels of antibody to peptide 18 by ELISA compared to prebleed controls, only one of four animals of the BALB/c strain generated neutralizing antibody at the lowest dilution tested (Table II). The lack of

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PCT/ÜS94/05142 correlation between specific peptide 18 antibody response and neutralizing activity was especially apparent in animals immunzed with PCLUS 3-18. Significant levels of peptide 18 specific antibody were elicited as determined by ELISA in all strains of mice immunized with PCLUS 3-18, and the animals of the ASES haplotype showed the strongest antibody response to this construct . Nevertheless, only animals of the H-2d haplotype made antibodies capable of neutralizing virus in vitro (9 of 10 animals), despite lower levels of antibody by ELISA. This finding suggests that the in vivo induction of neutralizing antibody by the B cell epitope (peptide 18) immunogen depends on other factors in addition to the level of help, such as the specificity of helper T cells, or other MHC-linked regulatory factors.

PCLUS 6-18 reproducibly elicited neutralizing antibody in all strains tested in which it elicited a significant antibody response by ELISA (Tables II and

III) . The geometric mean neutralizing antibody titers achieved in BALB/c (42.2) and B10.BR (32.0) correspond to levels of neutralizing activity directed to the V3 loop that have been found sufficient to protect chimpanzees from a live homologous viral challenge (30) .

Neutralization titration profiles from two separate experiments for each group of animals following a single immunization with PCLUS 6-18 are shown in Figure 2.

Each animal received 20 nanomoles of synthetic peptide emulsified in CFA (1:1) intraperitoneally in a volume of 0.1ml. Neutralizing activity is determined in a microculture syncytium-forming assay using the HIV-1 IIIB HX3 strain and is expressed as Vn/Vo where Vn is the mean number of syncytia forming units (SFU) in duplicate test wells and Vo the number of SFU in control wells incubated without test sera. Each curve represents serial two fold dilutions of individual mouse serum (designated by animal number) except the prebleed pool

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PCT/US94/05142 which includes all animals within a group. The two columns represent two separate experiments.

It is interesting to note that three of five BALB/c mice shown in the first panel had 90 % neutralizing titers greater than 64, which was the highest dilution tested. In addition, four of ten B10.BR mice shown in panels 3 and 4 exhibited neutralizing titers greater than 64. Over half (22/40) of all animals immunized with cluster peptide 6-18 demonstrated 50% neutralization of live virus at a dilution of 1:64 and all but one neutralized 50% of the virus at one of the dilutions tested. This single animal number 6269 B10.HTT had a negligible antibody response in a group that appeared to be poorly immunized. The mice that responded to the primary immunization were given a single boost with the same construct in CFA 37 or 49 weeks after the first immunization. This single boost produced remarkably high titers of neutralizing antibody with 90% neutralization occurring out to 1:2048-1:4096 in many animals and 1: 16,384 in some (Fig. 3 and Table IV ). These neutralizing titers against the homologous viral strain after just two immunizations are at least four to eight fold higher than the highest titers of other polyclonal sera induced by any immunization that we have ever observed (8,59). Moreover, the timing indicates that memory from the primary immunization lasted at least 11 months. Furthermore, in three of four strains boosted with PCLUS6-18 IIIB (SEQ. I.D. NO. 36), the sera were also capable of neutralizing the HIV30 1 MN strain, albeit at much lower titer (Table IV) .

Although mice of the H-2d haplotype produced some of the highest neutralization titers against HIV-1 IIIB (e.g. 1: 16,384) following a single boost, none of these sera showed cross-neutralizing activity against the MN strain.

In order to attempt to explain why some sera had high neutralizing activity and other sera with similar

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ELISA titers for the same short sequence did not, we compared affinity, isotype, fine specificity and other properties of neutralizing and nonneutralizing antibodies generated by each of the cluster peptide5 peptide-18 immunizations (summarized in Table III). It is important to note that all the sera tested, with the exception of that of one animal (B10.BR # 9770) , were capable of neutralizing the virus at some dilution as assessed by 50% inhibition compared to control sera, even if they were nonneutralizing by the criterion of 90% inhibition. Therefore it may be more difficult to differentiate specificity differences, since sera that are nonneutralizing at 90% inhibition may possess low levels of neutralizing antibodies which would blur the distinction.

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TABLE III

Properties of Neutralizing Antibodies Generated By Cluster Peptide-P18 Immunization

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TABLE III (continued)

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Table IV

Properties of Sera Boosted with 10 nanomole Cluster Peptide-P18

<td rowspan="3"> Cluster Peptide/ Mouse Strain</td><td rowspan="3"> Serum#</td><td colspan="2"> ELISA</td><td colspan="4" rowspan="2"> Neutralization TUB 1 MN</td>

<td> PCLUS 3-18 BALB/c</td><td> 6243</td><td> 0.44</td><td> 0.08</td><td> 8</td><td> 32</td><td></td><td></td>

<td> PCLUS 6-18 BALB/c</td><td> 6247</td><td> 2.27</td><td> 0.42</td><td> 4068</td><td> >32,768</td><td></td><td></td>

ELISA readings measured ar 405 nm and reciprocal dilution neutralization titers of individual mouse sera 39 - 52 weeks following a single boost with 10 nanomole of cluster peptide 3 or 6 in four different strains of mice. Neutralization of homologous virus at 90% and 50% endpoints is indicated in the column labeled ΙΠΒ indicating HTV-1 ΠΙΒ and cross neutralization in the column MN representing HIV-1 MN.

* - indicates negative at the lowest dilution tested,l:64.

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Results in column 4 show direct binding to HIV IIIBinfected cells by immunofluorescence. Three of five sera from animals immunized with cluster peptide 6-18 and capable of neutralizing virus by 90% in the syncytium-forming assay exhibited binding to virus infected cells, although there was no correlation between neutralizing titer and IFA titer. Two neutralizing sera from mice immunized with PCLUS 3-18, #6245, and PCLUS 6-18, #6249, failed to bind infected cells by IFA. In addition, one of two nonneutralizing sera, from mouse #9770 immunized with PCLUS 6-18, exhibited binding to virus-infected cells despite the absence of any neutralizing activity at the level of 50% inhibition, in the synctium forming assay. In this small sample we were not able to detect a difference between neutralizing and nonneutralizing sera by IFA.

Sera from animals immunized with the cluster peptides were tested for binding to rgp 120 in an ELISA assay. Note that it is only relevant to compare absorbance reading from the ELISA assays in Table III within columns and not between columns, since different reagents and assay conditions are employed for each type of ELISA. All immune sera tested bound rgpl20 whereas prebleed control sera did not, and there was no significant difference in level found between neutralizing and nonneutralizing sera. In addition no difference between sera could be found in IgM levels which bound P18 or rgp 120. In a small sample, the isotype of antibodies elicited to P18 was determined to be IgGj. None of the sera tested showed any antibodies of the isotype IgG2a. No correlation was found between isotype and neutralizing activity.

Since antibodies elicited to the T helper sites may play a role in a subsequent viral infection, it was important to determine if sera from animals immunized with the cluster peptide-peptide-18 constructs contained any antibodies to the cluster peptides themselves.

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PCT/US94/05142 sites were the lack of peptide 6 antibodies, neutralizing activity

Also, if antibodies to the helper neutralizing, these could account for correlation between binding to peptide 18 and neutralization. We tested this possibility in an ELISA 5 using plates coated with cluster peptide 3 alone or cluster peptide 6. None of the animal sera tested showed binding to PCLUS 3. In contrast, all animals immunized with PCLUS 6-18 produced antibodies that were reactive to the T helper site and the level of antibody produced 10 was proportional to their peptide 18 response, whereas none of the control animals produced anti-cluster However, it is unlikely that of these sera was due to antibodies to cluster peptide 6, because of its location 15 in the intracytoplasmic tail of gp 41, and the lack of cross-neutralization of the MN strain {Table III), as discussed further below. Therefore, the neutralizing activity musts be primarily directed to the P18 portion of the construct.

It remained possible that differences in neutralizing ability were due to differences in affinity of antibodies. To test this possibility, sera selected on the basis of their ability to neutralize virus in the quantitative infective syncytium-plaque forming assay were mixed with various concentrations of peptide 18 or rgp 120 and allowed to achieve solution phase equilibrium. Dilutions of competitor were allowed to reach solution phase equilibrium in an overnight incubation at 4°C with a 10’3dilution of each antiserum.

Free antibody was then determined by short term incubation on competing peptide 18 or rgp 120 coated plates respectively, in an ELISA assay. Concentrations required for 50% maximal competition (IC50), as estimates of Kd, were determined for each serum tested from the binding curves. Each experiment was repeated three to four times and representative results are shown in

Figure 4. Binding avidities (reciprocal of Kd) of all

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PCT/US94/05142 sera tested were over two logs higher (i.e. IC50 two logs lower) when tested with rgp 120 than with peptide 18 (Table III) . Binding avidities of neutralizing and nonneutralizing sera to peptide 18 were comparable, and any differences in avidity for rgp 120 were equivocable. In one case neutralizing serum from an animal immunized with cluster peptide 6-18, B10.BR mouse number 9772, showed a 5-fold lower binding avidity for rgp 120 than a corresponding nonneutralizing serum,

BALB/c number 6251, whereas another neutralizing serum in this same group, B10.A(5R) mouse number 9777, showed only a 1.4 fold lower binding avidity than corresponding nonneutralizing serum 6251. The fact that the binding curves were not as steep as expected reveals that these sera were not homogeneous or monoclonal in the population of antibodies binding to either peptide 18 or rgp 12 0, and heterogeneity may have influenced these results. However, the fact that neutralizing sera often had lower avidities than nonneutralizing sera suggests that neutralization does not correlate with higher average avidity to peptide or to recombinant gp 120.

To explore the possibility that fine specificity differences might explain differences in neutralizing activity of sera with comparable peptide binding activity, we used peptides with single amino acid substitutions, in which single residues from the HIV-1RF sequence replaced residues in the HIV-l-IIIB sequence, (see Table V, SEQ. I.D. NOS. 8-23) to test the effect of each residue on the binding of neutralizing and nonneutralizing sera from animals immunized with PCLUS 3-18 and PCLUS 6-18 (Figure 5).

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Table V

Substituted Peptides Used For Determination of Binding Specificity (37)

315

18ΙΠΒ(Ρ18) R

18RF *

329

I G K T G Q

18-1

18-2

18-3

18-4

18-5

18-6

18-7

18-8

18-9

18-10

18-11

18-12

18-13

18-14

18-15

T

K

Δ y

I γ

I

Û * Indicates a deletion $ The underlined amino acids are substitutions in the 18ΙΠΒ sequence.

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Substitutions at neither the amino nor the carboxyterminus of peptide 18 seemed to affect binding by either neutralizing sera, represented by the solid columns, or nonneutralizing sera, represented by the open columns, from animals immunized with PCLUS 3-18 or PCLUS 6-18. In fact, binding was enhanced over peptide 18 control when a tyrosine was substituted for a valine at position number 11 and substitutions at positions number 12, 13, 14, and 15 revealed a similar enhancement. Binding of both groups of sera was reduced when substitutions were made in the central loop region of the peptide 18 sequence PGRAF. This was not surprising since the sequence GPGR has been shown to be the binding site for neutralizing antibodies and maintains a well defined β-turn conformation (60). It was surprising that a substitution of alanine for glycine at residue 312, (peptide 18-5) did not influence binding. It was also surprising that a substitution of alanine for proline residue 313 (peptide 18-6) did not more completely abrogate binding despite its effect in disrupting the putative reverse turn conformation. The most interesting substitution was substitution position number 8, amino acid residue 315. Neutralizing sera retained binding, albeit significantly less than to unsubstituted peptide 18, to a peptide with an alanine for arginine substitution made at this residue, whereas nonneutralizing sera failed to bind to this substituted peptide. We examined the central loop region further with additional sera. We were able to reproduce this finding in 3 additional experiments. Results from such an experiment are represented in Figure 6. Both neutralizing and nonneutralizing sera from animals immunized with cluster peptide 3-18 (Fig. 6A) showed reduced binding to substitutions made at positions 6, 7,

8, and 9. However, neutralizing sera retained a slight degree of binding to the peptide substituted at position number 8, whereas nonneutralizing sera failed to bind.

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Similarly in the lower panel, neutralizing and nonneutralizing sera from animals immunized with PCLUS 6-18 showed a dramatic reduction in binding to peptides with substitutions at position 6, 7, 8, and 9. Again, however, neutralizing sera retained a higher level of binding to the peptide with alanine for arginine substituted at position 8. In another experiment, the difference in effect of position 8 between neutralizing and nonneutralizing sera was more marked, although not as many substitutions were tested. The implications of an alanine for arginine substitution at residue 315 are unclear since it is the neutralizing sera which retain modest binding. However this result combined with the slightly greater effect of substitutions at positions 6 and 7 on binding of neutralizing sera than nonneutralizing sera suggests that neutralizing sera may be more focused on the tip of the loop, and nonneutralizing more focused on the carboxyl side adjacent to the central loop. Thus, a subtle difference in fine specificity may explain the difference in neutralizing activity among sera with comparable peptide and gp 120 binding activity.

This study demonstrates the utility of combining neutralizing B cell epitopes with a cluster of immunodominant T- helper sites for the purpose of constructing synthetic peptide vaccines with enhanced immunogenicity in multiple MHC types. The T-helper sites chosen for this study encompass multideterminant regions of the HIV envelope protein gpl60 previously shown to be recognized by mice of multiple H-2 types as well as human T cells from HIV-infected patients representing a broad array of HLA types. Our strategy was to link each cluster peptide to a short synthetic peptide (peptide 18) , previously identified to be an immunodominant site recognized by CD8 cytotoxic T cells in association with class I molecules, and found within the V3 loop or principal neutralizing determinant region

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PCT/US94/05142 of the HIV-IIIB envelope protein. These constructs, PCLUS 3-18, PCLUS 4-18, and PCLUS 6-18 (Table I) all elicited peptide 18 specific antibodies capable of binding rgpl20 following a single immunization.

Antibodies from mice representing four different MHC haplotypes immunized with one of these constructs, PCLUS 6-18, were capable of neutralizing virus. In 24 of 35 (69%) of the total animals of all strains immunized with PCLUS 6-18, antibodies elicited following a single immunization inhibited the infectivity of the homologous virus by greater than 90%. In three of the strains tested PCLUS 6-18 elicited neutralizing antibody in 8090% of the animals showing a peptide-18 specific response by ELISA. It is interesting to note that in one experiment the reciprocal geometric mean neutralizing antibody titer achieved in two haplotypes of mice, 42.2 in H-2d and 32.0 in H-2k, was a level approaching the levels known to be protective against an intravenous virus challenge in chimpanzees (30) . This mean value is a low estimate since a number of animals in each group had neutralizing titers greater than 64, which was the highest dilution tested. Moreover, a single boost resulted in 90% neutralization titers of 1: 1000 to 1: 16,000, much higher than seen in our experience with any other immunization protocol (8,59). PCLUS 3-18 elicited peptide specific antibody in all strains tested, but only in animals of the H-2dhaplotype were these antibodies consistently neutralizing (nine of ten mice).

The level of antibody binding activity by ELISA to either peptide 18 or rgp 120 was not predictive of whether or not antibodies elicited by the cluster peptide-peptide 18 construct would be neutralizing. This suggests that other factors, in addition to the level of T cell help enhancing the magnitude of the antibody response influence the qualitative response to the construct. In order to investigate the mechanism

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PCT/US94/05142 resulting in marked differences in neutralizing activity among sera with comparable peptide 18 binding activity by ELISA, we compared the specificity, affinity, and isotype of the antibodies. Interestingly, all sera that contained peptide 18 specific antibodies also contained high levels of antibodies that bound to envelope protein rgp 120 even though animals were immunized with peptide. This finding suggest that the preferred conformation of the short synthetic sequence of peptide 18 mimics to some degree that of the corresponding region in the larger envelope protein. Although 3 of 5 neutralizing sera tested from animals immunized with PCLUS 6-peptide 18 bound virus infected cells expressing native gpl20 in an IFA assay, we could not find any correlation between neutralization titer and binding by IFA. Furthermore, contrary to expectation, neutralizing sera exhibited slightly lower binding avidities to rgp 120 than nonneutralizing sera. Clearly other factors, in addition to the magnitude and affinity of binding to the

V3 loop of gpl20, influence neutralization.

To investigate this we looked for differences in isotype and fine specificity. 'No isotype differences were found. Both neutralizing and nonneutralizing sera showed reduced binding to substitutions in the central loop region of peptide 18 (PGRAF), although results suggested that neutralizing sera may be more focused on the tip of the loop, and nonneutralizing antibodies more focused on the carboxyl side adjacent to the central loop, in agreement with the experience of Javaherian et al.(61).

Results in this study are compatible with previous results in which a synthetic vaccine was made by coupling a T helper site, Tl peptide residues 421-436, to a longer peptide called SP10, residues 296-314 located within the V3 loop (62,63). Animals immunized with this construct developed both proliferative responses to the gp 120 envelope protein and

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PCT/US94/05142 neutralizing antibody after a series of immunizations employing different immunization regimens. Tl is contained within PCLUS 3, but does not represent a multideterminant region, and SP10 overlaps peptide 18 but lacks eight residues toward the carboxyl terminus of the central loop. Although the V3 loop is not the only site on the viral envelope that elicits neutralizing antibodies (64-67), it appears to be the major one that can be defined by a linear peptide sequence and against which antibodies can neutralize virus after it has bound its cellular receptor, the CD4 molecule (24,61,68,69).

The hypervariability of this region raises questions about how many variants of this sequence must be included in a vaccine. PCR studies of - virus from

North American and European isolates suggest that there is a consensus sequence that is relatively conserved that is most like the MN or SC isolates (24) . In another independent study of infected individuals in North America and Europe, the majority of those tested were shown to possess antibody reactive with an MN-like variant of the V3 loop (70,71). A relatively conserved portion of the loop, GPGRAF, has been shown to elicit antibodies that neutralize four of seven isolates of HIV tested (61). Isolate-specific neutralization is commonly found. The presence of neutralization escape mutants in infected patients may involve sequence variation within the V3 loop itself as well as sequence variation outside the loop leading to conformational changes (33,72). It is likely that a synthetic peptide vaccine must contain multiple constructs containing divergent V3 loop peptides that produce neutralizing antibodies of the right conformational specificity to a large number of transmitting isolates.

We have also shown in this study that a booster immunization with PCLUS 6-18 given up to 49 weeks post vaccination leads to a response 2.5-10 times the magnitude of the initial response by ELISA. Strikingly

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PCT/US94/05142 this rise in antibody levels correlates with an even higher rise in neutralization titers up to 1:1000 to 1:16,000 for 90 % neutralization, and even higher for 50 % neutralization (Fig. 3 and Table IV ). Lower titered crossreactivity on the MN isolate was also observed. At five months following the boost, antibody titers to P18 as measured by ELISA have remained at a high plateau.

An important question was to determine if immunization with PCLUS 3-18 or 6-18 elicited antibodies that were directed to the T helper sites themselves. It is unlikely that antibodies directed to T helper sites would block the T cell receptor from recognizing the site presented by the antigen presenting cell, because antibody directed to whole proteins has previously been shown to enhance uptake of the molecule and presentation to T cells, and it has usually not been possible to block presentation of antigen to T helper cells by antipeptide antibodies directed to a T cell epitope (73-76), although exceptions have been reported (77,78). We did not observe any diminution of response in animals immunized with cluster peptide 6-18 following a single boost 36-52 weeks post vaccination even though we have demonstrated that some antibodies are elicited to the T helper region of this construct. Another potential concern is antibody mediated antibody enhancement of viral infectivity, mediated via the Fc receptor and complement receptor (16,79). It is presently unclear to which site(s) enhancing antibodies are elicited during the natural infection, although some have been found to bind to the N-terminal portion of gp 41 (11,79).

Antibodies to the region included in PCLUS 3 have not been found to enhance infectivity. Cluster peptide 6 is located within the intracytoplasmic gp41 region of the virus envelope protein and therefore should be less likely to be either enhancing or neutralizing. Finally, we think it unlikely that much of the neutralizing acitvity was directed against the helper cluster

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PCT/US94/05142 peptides, for two reasons. First, we could not detect antibodies to PCLUS 3 by ELISA in the neutralizing sera from animals immunized with the PCLUS3-18 construct. In animals immunized with PCLUS6-18, some antibodies were detected to PCLUS 6, but since these are directed to the intracytoplasmic tail of gp41 which is not believed to be exposed on the virion, it is unlikely that they could neutralize virus. Second, antibodies to either PCLUS 3 or PCLUS 6, if neutralizing, would be expected to cross10 neutralize the MN strain of HIV-1, since these regions are relatively conserved compared to the P18 region, against which neutralizing antibodies are generally type-specific. Thus, the lack of cross-neutralization (Table III) indicates that the neutralizing activity is directed primarily against P18 and not the helper sites. In addition, antibodies to the Tl peptide, which represents a major portion of the PCLUS 3 peptide, were found not to correlate with neutralization in another study (80).

EXAMPLE II: IMMUNIZATION OF MICE TO ELICIT CTL AGAINST

HIV-1

Since HIV can spread ' via cell to cell transmission, the ability to elicit a viral specific CTL response might be important for a synthetic peptide vaccine in order to be an effective modality of immunotherapy.

The peptides described in Example I contain contain a CTL epitope in addition to a Th epitope and PND. Thus, the peptides were also tested for induction of CTL activity in mice.

Here, we address the question with a single immunization using a saponin adjuvant, QS21 (103), which does not require an emulsion, allowing us to test the requirement for covalent linkage between helper and CTL epitopes under limiting conditions. We also addressed the MHC linkage of helper activity by using congenic mouse strains differing in class II MHC but sharing the

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H-2Dd class I molecule presenting the CTL determinant. We have previously described the construction of synthetic peptides encompassing immunodominant Th peptides spanning multidéterminant regions (54) from the

HIV envelope protein gplGO (42) . These so-called cluster peptides, each consisting of a cluster of overlapping determinants, were found to induce in vitro T cell proliferation and cytokine production in mice and humans of multiple MHC types, respectively (42) . Three cluster peptides were used in the current study: PCLUS3 (residues 421-444, KQIINMWQEVGKAMYAPPISGQIR, SEQ. I.D. NO. 24), PCLUS4 (residues 476-499, RDNWRSELYKYKWKIEPLGVAPT, SEQ. I.D. NO. 25), and PCLUS6 (residues 821-853, AVAEGTDRVIEWQGAYRAIRHIPRRIRQGLER,

SEQ. I.D. NO. 26), in which the HIV-1 IIIB numbering is according to the Los Alamos database (43) (7 less than that of Ratner et al (44) used in previous publications (42)) .

To design a peptide vaccine immunogenic in multiple

MHC types and to investigate the mechanisms of CTL priming in vivo, we co-linearly synthesized each of the cluster peptides at the N-terminus of an immunodominant CTL determinant, P18 (45) (residues 308-322,

RIQRGPGRAFVTIGK, SEQ. I.D. NO. 7), previously identified to be recognized by murine CD8+ CTL of four distinct MHC types (104) as well as human T cells from HIV-infected patients representing a broad array of HLA types (46) . The P18 peptide corresponds to part of the gpl6 0 V3 loop and principal neutralizing determinant (PND) region of gpl60 (21-23), and is also presented by a class II MHC molecule (I-Ad) to helper T cells in mice of appropriate MHC type (105).

The cluster peptide-peptide-18 constructs were synthesized on an automated peptide synthesizer (Model.430A; Applied Biosystems, Foster City, CA) utilizing t-boc chemistry (51). The peptides were cleaved from the resin with HF and initially purified by

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PCT/US94/OS142 size exclusion chromatography. Purification to single peaks was achieved by reverse-phase HPLC on gbondapack reverse-phase Cl 8 columns (Waters™ Associates, Milford, MA). Mice 8-20 weeks old were immunized subcutaneously at the tail base with 20 nmoles of each peptide mixed with QS21 (15 gg) , the highly purified saponin fraction from the soap bark tree Quillaja saponaria, which retains the greatest adjuvant activity but is non-toxic (103). Two weeks after a single immunization, immune * spleen cells from B10.D2 (H-2d) (Fig. 7A) , B10.A(5R) (H2b) (Fig. 7B) , or B10.S(9R) (H-214) (Fig. 7C) mice (5xl06/ml in 24-well culture plates in complete T cell medium (1:1 mixture of RPMI 1640 and EHAA medium containing 10% FCS,- 2mM L-glutamine, 100 U/ml penicillin, 100 gg/ml streptomycin, and 5xl0'5M 2-ME) ) were restimulated for 6 days in vitro with 0.1 μΜ of P18 and 10% Con A supernatant-containing medium (Rat T stim; Collaborative Research, Inc., Bedford, MA). Cytolytic activity of in vitro secondary CTL was measured as described previously (104) using a 6 hr. assay with 51Crlabeled targets. The fibroblast targets were BALB/c.3T3 transfectants (H-2d, class I MHC+, class- II MHC' ) expressing the whole gpl60 protein endogenously (cell line 15-12 (ref (45)). Similar results were obtained with control BALB/c 3T3 cells transfected with only the neomycin resistance gene (18neo cells) pulsed with 1 μΜ P18 (data not shown) . As a control for both gpl60 transfectants and peptide-pulsed 18neo cells, background lysis using unpulsed 18neo target cells in the absence of specific peptide was less than 8 %. Effectors were cocultured with peptide-pulsed targets at the indicated E:T ratios. The percent of specific 51Cr release was calculated as 100 X ((experimental release - spontaneous release)/(maximum release - spontaneous release)).

Maximum release was determined from supernatants of cells that were lysed by the addition of 5% Triton X100. Spontaneous release was determined from targets

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<td> cells incubated without</td><td> added effectors</td><td> cells .</td><td> The</td>

<td> assay was performed in</td><td> triplicate, with</td><td> 5000</td><td> target</td>

<td> cells per well.</td><td></td><td></td><td></td>

<td colspan="2"> Thus, mice of three haplotypes, B10.D2</td><td> (H-2d;</td><td> AdEd) ,</td>

<td> differing in class II</td><td> but sharing the</td><td> class</td><td> I Dd</td>

molecule, were immunized once subcutaneously at the tail base with purified saponin (QS21) containing the compound peptides, PCLUS3-18, PCLUS4-18, or PCLUS6-18, or P18 alone. When their immune spleen cells were restimulated in vitro with P18 in the presence of IL2, we obtained CTL that could kill both transfected BALB/c 3T3 fibrobrast targets expressing endogenously the whole gpl60 protein (called 15-12; ref (45)) (Fig. 7) but not control BALB/c 3T3 fibrobrast targets (called 18neo, transfected with the neomycin resistance gene alone). They could also kill the gpl60-negative 18neo cells pulsed with peptide P18 (see Fig. 8), evidence that the killing was specific for the P18 moiety. The mice primed in vivo by the compound peptides generated strong CTL activity against 15-12 as well as P18 pulsed targets at effector to target (E:T) ratios as low as 7:1, and reaching levels of 45-75% specific lysis at E:T ratios of 60:1 (Fig.7A,B,C). In contrast, mice immunized with

Pl8 alone exhibited only marginal CTL activity even at maximal E:T ratios of 60:1. The shift in E:T ratio curves for killing 15-12 targets indicates more than a 10-fold greater number of CTL lytic units in mice primed with the compound peptide compared to mice primed in vivo with P18 alone. Unimmunized mice or mice immunized with adjuvant or peptide alone also failed to give rise to CTL. Also, the cluster peptides without the P18 component failed to prime specific CTL. The finding of markedly diminished or absent CD8+ CTL response to Pl8 in mice immunized with P18 alone mixed with QS21 raised the possibility that the in vivo priming for P18 specific CTL requires CD4 class Il-restricted help and

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PCT/US94/05142 that this help is provided by the immunization with the compound peptide containing cluster Th determinants.

To test whether covalent linkage of the helper determinant to the CTL determinant was required to prime mice in vivo for a CTL response, or whether a mixture of peptides not covalently linked was sufficient, we immunized B10.D2 or B10.A(5R) mice with compound peptides PCLUS3-18 or PCLUS4-18, respectively, or with mixtures of free peptides PCLUS3 or PCLUS4 and P18, or * P18 alone in QS21 adjuvant (Figs. 8A-8D) . Mice were immunized with PCLUS3-18 or PCLUS4-18, respectively, or mixtures of PCLUS3 or PCLUS4 and P18, or P18 alone, in QS21 adjuvant. Spleen cells from the immunized mice were restimulated for 6 days with 0.1 μΜ P18 and rIL2 (10 units/ml, Genzyme™, Cambridge, MA) . The effectors were tested on the gpl60 transfected line 15-12 (Figs. 8A, 8B) or neo-only transfected 3T3 fibroblasts (18neo) pulsed with P18 (ΙμΜ overnight) (Figs. 8C, 8D).

Background lysis on control 18neo fibroblasts in the absence of peptide was less than 8 %. SEM of triplicate wells was less than 7.2 % of the mean.

Thus, spleen cells immunized in vivo with the peptides of the present invention were restimulated with P18 and IL-2, which compensates for any deficiency in T25 cell help in vitro (since there are no Th cells present in the culture). Cytolytic activity was measured both on the transfected 15-12 fibroblast cell line endogenously expressing gpl60, and on control 18neo fibroblasts pulsed with P18 or no peptide. No lysis was

0 observed on the control targets in the absence of peptide. Surprisingly, the CD8+ CTL activity of immune spleen cells from mice immunized with the mixture or P18 alone was negligible, whereas the compound peptide immunization elicited a strong CTL response in both strains (Figs. 8A-8D). The lysis of targets pulsed only with P18, taken with the lack of CTL activity against targets pulsed with PCLUS3 or PCLUS4, indicates that the

><***», WO 94/26785

PCT/US94/05142 linkage requirement applies to induction of CTL activity specifically against the P18 determinant, and therefore is not due to some other activity that might be induced by the compound peptide and measured on the gpl605 expresssing targets. These results indicate that covalent linkage of the cluster peptides as helper sites to the CTL site in the compound peptides PCLUS3-18 and PCLUS4-18 was required for priming of CTL in vivo. This finding was consistently reproducible in three independent experiments.

Because immunization with the compound peptide might prime CD4+ CTL, we determined the phenotype of the specific CTL primed by the peptides. Figure 9A shows the results when the CTL from B10.D2 and B10.A(5R) mice 15 immunized with 20 nmoles of the compound peptides in 15 μg QS21, after restimulation with 0.1 μΜ P18 plus rIL-2, were treated with either with anti-CD8 monoclonal antibody (3.155; rat IgM) (114) plus complement (solid bar) or anti-CD4 monoclonal antibody (RL.174; rat IgM) (113) plus complement (open bar), or complement alone (not shown) , as described previously (28) , and tested on P18 pulsed 18neo as targets.

As shown in Fig. 9A, the CTL were conventional CD8+CD4* CTL. Also as the targets express only class I, not class II, MHC molecules, the CTL must be restricted by class I MHC molecules. The restriction was mapped to the Dd molecule using L-cells transfected with each H-2d class I molecule.

In contrast, the helper cells induced by the PCLUS-18 constructs were CD4 + , at least as measured in the in vitro stimulation of the immune spleen cells. For example, B10.A(5R) mice were immunized with 20 nmoles of PCLUS4-18, and their spleen cells were treated with or without anti-CD4 (RL 174, reference 113) plus complement before being restimulated for 6 days with PCLUS4-18 or

Pl8 alone in the presence or absence of recombinant IL-2 (rIL-2, 10 U/ml). The resulting effector cells were

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PCT/US94/05142 tested on the gpl60-expressing 15-12 BALB/c fibroblast cell line or on neo-only transfected fibroblast cells (not shown) as targets (Fig. 9B). No lysis was observed on the neo-only transfected control targets (less than

4.2 %) . SEM of triplicate wells was less than 7.3% of the mean. As a control, PCLUS4 alone (not linked to P18) in the presence of IL-2 did not induce any CTL activity. PCLUS4-18, but not P18 alone, stimulated the induction of CTL activity in the absence of rIL-2 to replace help (Fig. 9B). This result suggested that the PCLUS4-18 peptide was eliciting T-cell help in the restimulation culture, making exogenous IL-2 unnecessary. Elimination of CD4+ cells prevented the ί· induction of CTL activity in absence of IL-2, but not in the presence of IL-2 (Fig. 9B). Therefore, the helper cells induced in cultures of cells immune to PCLUS4-18 and restimulated with PCLUS4-18 were CD4-positive. The induction of CTL activity in the cultures of anti-CD4treated cells in the presence of rIL-2 indicates that the antibody and complement treatment did not affect the CTL precursors themselves. Thus, the CTL precursors, like the CTL effector cells (Fig. 9A) were CD4-negative.

Thus, we have shown a requirement for helper-CTL determinant linkage in vivo for induction of CTL, which had never been demonstrated before for CTL, in contrast to Th-B-cell cooperation, for which a requirement for cognate help in vivo has been widely recognized for many years. This result appears to contrast with two recent studies with mixtures of helper and CTL antigenic peptides, in which covalent linkage was not obligatory (90,102) . We may reconcile these findings by suggesting that in the former study (90), cosequestration of the peptides in an adjuvant emulsion kept them physically together in oil microdroplets, and that in the latter study (102) , multiple high doses of peptide used were able to overcome the inherent disadvantage of the unlinked mixture. This explanation is consistent with

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PCT/US94/05142 the requirement for proximity or presentation on the same presenting cell demonstrated by the requirement for helper and CTL determinants to be on the same skin graft to induce a rejection response in vivo (89) . The explanation is also consistent with our findings in vitro that in the confines of a culture well, a mixture of cluster peptide and P18 is sufficient to elicit a CTL response without added IL-2 almost as efficiently as the covalent construct. The lower dose without adjuvant emulsion may more closely mimic the case in natural infection. As the peptide which induces the strongest CTL response is different from strain to strain (Figs. 7A-7C), the enhancement of CTL response by compound peptide cannot be accounted for simply by the effects of the helper site on resistance or susceptibility of the peptide to enzymatic degradation in vivo. Indeed, the reproducible difference in responsiveness to different peptide constructs among congenic recombinant mouse strains differing in class II but sharing the same H-2Dd class I molecule implies that the helper T-cells are class II MHC restricted and that the cluster peptides are not presented equally by all the class II molecules. Nevertheless, the use of the cluster peptides allows much broader helper recognition among mice of different

MHC types than would be elicited by single helper determinants (42).

The mechanism of induction of CTL in vivo by compound peptides is likely to be that the longer peptides are taken up by specialized class II-expressing antigen30 presenting cells (APC), implicated in CTL induction (100,106,107), probably at the injection site or in draining lymph nodes, before degradation of the peptides by protease in serum or extra-cellular fluid, so the same cell may present both the CTL and Th epitopes through class I and class II MHC, respectively. This presentation may be more efficient than others in which the two epitopes are presented by the different APC

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PCT/US94/O5142 independently. The greater efficiency of presentation by the same APC may be because it brings the helper T cell and the CTL precursor together, for more effective transmission of small quantities of labile lymphokines, or, as suggested by Gill and Lafferty (108), because the APC is activated by the helper cell, and then in turn is more effective at presenting antigen to the CTL. In the former case, the presentation events to both cells would have to be close in time, whereas in the latter case, they could be separated in time. In either case, the same APC would be more efficient than two separate APC, and therefore the linked determinants would be more effective than ones that could diffuse apart once injected in vivo. This is consistent with the recent observation in which specialized APC expressing class II MHC simultaneously present extracellular antigens through both class I and class II MHC pathways to CTL and Th, respectively (107) , and with the skin graft experiments cited earlier (89) . QS21 may be able to penetrate cell membrane and introduce antigen into the cytoplasm, from which it can enter the MHC class I presentation pathway (103). Whichever of the above mechanisms holds, the stimulation of both cells by the same APC should facilitate the delivery of help.

Progression of HIV-1 infection toward AIDS appears to correlate with a shift from Thi to Th2 predominance in the HIV-1 specific cytokine response (109,110). In a previous study from our lab, reduction in CTL response to P18 due to concurrent schistosome infection appeared to correlate with a shift from Thi, producing IL2, to Th2 predominance (111) . Thi cells are thought to provide the CD4+ class Il-restricted help for CTL priming, whereas Th2 cells may secrete cytokines that inhibit CTL generation. Therefore, immunization of HIV35 1 carriers for immunotherapy should be most effective if it boosts both Thi CD4+ cells and CTL, as these compound constructs are intended to do.

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Help for induction of P18-specific neutralizing antibody was also observed following a single immunization in mice of four different haplotypes with these compound constructs containing cluster peptides (Example I). Although the immunization experiments presented here had to be carried out in experimental animals, the fact that the same epitopes are also recognized by human helper T cell and CTLs with more than one histocompatibility complex (HLA) class II or class I molecule (42,46), suggests that the same approach should be applicable to human immunization. Cluster 3 and 4 have sequences that are relatively conserved among North America and European isolates of HIV-1, and CLUSTER 6 spans the boundary between conserved and variable sequences (43).

Our current results, using recombinant mice differing in class II MHC molecules, suggest that Th determinants must be covalently attached to the CTL determinant on a single peptide to facilitate T-T collaboration and CD4+ class II-restricted help for priming of CTL response in vivo. Also, these cluster peptide-P18 constructs, previously shown to elicit high titers of HIVneutralizing antibodies (Example I, above) are useful immune constructs to elicit CTL as well as neutralizing antibodies to HIV in individuals of multiple MHC types. EXAMPLE III: BROAD IMMUNE RESPONSE ELICITED BY CLUSTER

PEPTIDES ATTACHED TO THE P18 PEPTIDE OF HIV-1 VARIANT MN

The hypervariability of the V3 loop of the HIV-1 virus raises the concern that vaccines directed to particular amino acid sequences in this region might not provide protection against the broad assortment of strains to which individuals might be exposed. One approach to overcoming the problem of strain variation is to immunize an individual with a preparation which comprises a mixture of several peptides, each directed against a different strain of the target pathogen. In order to assess the efficacy of peptides of the present

PCT/US94/05142

WO 94/26785 invention against additional strains of HIV-1, we prepared a series of cluster peptides attached to the P18 peptide derived from the V3 loop of the MN strain of HIV-1.

The peptides were prepared as described in the

General Methods, above. The sequences of the PCLUS-18MN peptides are presented in Table VI (SEQ. I.D. NOS. 2733) .

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Table VI

PCLUS-18MN peptides®

PCLUS1-18MN

EQMHEDIISLWDQSLKPCVKRIHIGPGRAFYTTKN

PCLUS3-18MN

KQIINMWQEVGKAMYAPPISGQIRRIHIGPGRAFYTTKN

PCLUS4-18MN

RDNWRSELYKYKWKIEPLGVAPTRIHIGPGRAFYTTKN

PCLUS6-I8MN

AVAEGTDRVIEWQGAYRAIRHIPRRIRQGLERRIHIGPGRAFYTTKN

PCLUS6.1-18MN

DRVIEWQGAYRAIRHIPRRIRQGLERRIHIGPGRAFYTTKN

P53-18MN

DRVIEWQGAYRAIRRIHIGPGRAFYTTKN

P55-18MN

AQGAYRAIRHIPRRIRRIHIGPGRAFYTTKN ® P18MN portion = RIHIGPGRAFYTTKN, PCLUS portion as described in Table I

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Four strains of mice representing four different haplotypes (See Example I, Table II) were immunized intraperitoneally with 20 nanomoles of P18MN, PCLUS318MN or PCLUS6-18MN peptide emulsified in Complete

Freund's Adjuvant (CFA) as described in Example I. Seven to eight weeks later, the mice were boosted with a second immunization, again with 20 nmoles i.p., and AbN titers against the HIV-1 strain MN were determined. The results of this experiment are shown in Figure 10.

* In a similar experiment comparing the peptides

PCLUS6-18MN and PCLUS6.1-18MN, mice were immunized as described above, but using those peptides. The results of this experiment are shown in Figures 11A-11H.

From these two experiments, it is concluded that

PCLUS3-18MN, PCLUS6-18MN and PCLUS6.1-18MN induce levels of AbN with a titer greater than 1:64 in all of the strains of mice tested, except that PCLUS3-18MN does not induce an immune response in BALB/c mice. Thus, the H-2d haplotype is not responsive to the PCLUS3-18MN peptide.

CTL response to the PCLUS-18MN peptides was investigated using the pl8MN and PCLUS3-18MN peptides. Primary immunization of groups of BALB/c mice was performed using 20 nmole of the peptide emulsified 1:1 with one of the following adjuvants: Alum, Incomplete

Freund's Adjuvant (IFA), QS 21 (described above), DOTAP (Boehringer Mannheim™ Biochimica, cat. no. 1202 375;

DOTAP is a lipofection reagent for the introducing macromolecules into cells through the plasma membrane.) and C259/763 (C259/763 is a proprietary substance provided by Dr. Fredrick Durr of Lederle Laboratories).

After two to three weeks, the mice were boosted with nmole of peptide and after an additional two to three weeks, spleens from two mice per group were removed and stimulated in vitro, as described in the above Example

II. Six days later, the cells were harvested and tested in a conventional cytotoxic T cell assay, again as described in Example II. Results are shown in Figures

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12A-12B.

An additional experiment was performed to assess the ability of the PCLUS6-18MN peptide to elicit a CTL response. Immunizations similar to those used above to elicit CTL to PCLUS3-18MN were used, comparing in this second case the responses to P18MN and PCLUS6-18MN and PCLUS6.1-18MN. However, the booster immunization was performed using 20 nmole of peptide and spleen cells were assayed for content of CTL after in vitro stimulation with peptide P18 and IL-2, the latter to provide non-specific help. The results of this experiment are shown in Figures 13A-13B.

As is clearly shown by the CTL experiments, the PCLUS-18MN peptides are more effective in eliciting a

CTL response than the P18MN peptide. The CTL response is produced in mice having a broad range of different MHC haplotypes. Furthermore, the superior efficacy of the PCLUS-18MN peptides in producing the CTL response is demonstrable in a variety of adjuvants.

Although the hypervariability of the V3 loop raises concerns for the design of a vaccine aimed at eliciting neutralizing antibodies and CTL specific for this region, it is encouraging that the same constructs with the P18 region from the HIV-1 MN isolate, which is representative of the most prevalent strains in Europe and North America (24,71), were also found to prime in vivo for a CTL response and to elicit a neutralizing antibody response against the MN variant. Also, recently, we found that CTL populations with broad specificities with respect to virus strain could be generated by restimulation of IIIB-gpl60 primed murine spleen cells with MN-type peptide with an aliphatic substitution at one position (112).

As demonstrated by the Examples hereinabove, the peptides of the present invention, which elicit efficient linked help for induction of both neutralizing antibodies and CTL in hosts presenting a broad range of

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MHC haplotypes should be considered as vaccine candidates for prevention or immunotherapy of AIDS. The approach presented here, that is, covalent attachment of epitopes constituting Th epitopes, CTL epitopes and PND for neutralizing antibodies into a single immunogenic polypeptide that is effective in producing all of these immune responses in a plurality of hosts having a broad range of MHC types, can be applied to other pathogens.

In particular, it is expected that this approach can be 10 ‘ applied to the development of vaccines against other viral pathogens, such as, but not limited to cytomegalovirus, hepatitis viruses, HTLV-I, rabies virus and the like. Furthermore, vaccines against viruses having non-human hosts, such as Feline Leukemia Virus, 15 Feline Immunodeficiency Virus and the like could also be produced using the approach of the present invention. EXAMPLE IV: ELICITING A BROAD IMMUNE RESPONSE TO MALARIA

CIRCUMSPROROZOITE ANTIGEN

U.S. Patent 5,028,425 describes the production of a 20 synthetic vaccine directed to the circumsporozoite (CS) protein of Plasmodium falciparum. That vaccine consists of a peptide immunogen which contains a CTL epitope from the CS antigen. U.S. Patent 4,886,782 describes a second synthetic vaccine directed against the CS protein which consists of a Th epitope that is linked to an AbN epitope.

In order to make a peptide vaccine which is recognized by a broad range of MHC types that will elicit a Th response, a CTL response and a high titer

AbN response, a peptide having the the sequence

PSDKKIEQYLKKIKNSISCNP (NANP) 5NAKPKDELDYENDIEKKICKMEKCS (SEQ. I.D. 35) is synthesized as described above. The peptide is administered to mice of differing MHC haplotypes (strains B10.D2, B10A(5R) and B10.SOR)) as described in Example II. AbN and CTL response is evaluated as described in Examples I and II,

216°

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PCT/US94/05142 respectively.

EXAMPLE V: CLINICAL TRIALS IN HUMAN SUBJECTS

The peptides of the present invention can be administered to human subjects as either a prophylactic 5 or therapeutic vaccine. Initially, clinical trials to evaluate the safety and maximum tolerated dose are conducted in human patients who are already infected with HIV. Thus, this example describes an experiment which provides preliminary evidence of the efficacy of 10 a vaccine formulation in a therapeutic mode.

The peptides of the present invention can be formulated into pharmaceutical compositions using the well-known materials and methods in the art. For instance, the peptides can be mixed with any sterile, 15 pharmaceutically acceptable carrier solution, e.g. saline, and packaged in syringe bottles for purposes of preparing an injectable preparation.

The dosage of the peptide that is administered can be also be determined by methods known in the 20 pharmaceutical arts. The actual dosages administered will vary depending upon various pharmacokinetic properties of the peptide, r including half-life, by various tissues, route of and the like. Typically, peptide pharmaceuticals are administered in amounts ranging from 0.1 to 50 ^g/kg body weight of the subject.

In the clinical trial described herein, the experimental cohort consists of HIV-infected patients, ages 18-75, with CD4+ cell counts of >600 cells/ml. 30 Consideration is given to requiring that the patients entered into the trial should present with evidence of intact T cell immune function, as measured by response to a recall antigen, for example influenza virus, as described in reference 57, in an IL-2 production assay. 35 Patients should be off anti-retroviral therapy during the initial four months of the trial. If, starting 3 months after entry into the trial, CD4+ cell counts fall sequestration administration,

PCT/US94/05142

WO 94/26785 below 500 cells/ml, and remains so for one month, then standard anti-retroviral therapy will be offered to the patients.

Peptides PCLUS3-18 and PCLUS6.1-18 are administered 5 to patients subcutaneously in Montanide™ ISA-51 adjuvant in a formulation prepared under GMP standards by Seppic™ Inc. (Fairfield, NJ). Vaccine is administered on day 0, and booster immunizations are given at 1, 3, 6, 9 and 12 months. The following immunization groups are made:

10* 1. 80 pg PCLUS3-18

2. 80 pg PCLUS6.1-18

3. 160 pg PCLUS3-18

4. 160 pg PCLUS6.1-18

5. 80 pg PCLUS3-18 + 80 gg,.PCLUS6.1-18

6. 160 pg PCLUS3-18 + 160 pg PCLUS6.1-18

Patients are first entered into groups 1 and 2, then into groups 3 and 4, then group 5 and finally into group

6. As experience is gained with the administration of the peptide to humans, patients in groups 1-4 might be permitted to receive the combination of PCLUS3-18 and PCLUS6.1-18, after a 6 month regimen of one or the other of the individual peptides.

A variety of immune system parameters is monitored in each patient, including:

routine chemistries and hematologic parameters;

Examination for toxicology and opportunistic infections;

Lymphocyte subsets;

Serum HIV p24 antigen levels;

Serum, plasma and cells are periodically frozen for measurement of viral load; measurement is by assays that are state of the art currently, including PCRbased assays ;

Measurement of antibodies specific to the peptides ^VO 94/26785

PCT/US94/05142 and to other determinants on HIV;

Determination of HIV neutralizing antibody titer;

Cytokine production, including interleukin-2 production, in response to mitogens, alloantigens, common antigens (e.g. influenza) and HIV antigens ;

Measurement of CTL activity against HIV antigens;

Skin tests for immune response to antigens other than those employed in testing for cytokine response.

Techniques for each of these assays are routine in the art.

EXAMPLE VI : USE OF THE PEPTIDES OF THE PRESENT INVENTION

IN DIAGNOSTIC ASSAYS

It is of course readily apparent to one of skill in the art that the peptides of the present invention, in addition to having utility as immunogens for eliciting a broad immune response, can also be employed in a diagnostic fashion to assay for antibody and CTL function in a patient.

The basic formats for antibody assays are well-known in the art, among them such solid-phase assays as ELISA, RIA and the like. In such solid-phase assays, the peptide of the present invention is attached to an insoluble substrate and the substrate, with the peptide bound thereto is contacted with a sample to be tested, under conditions such that if specific anti-peptide antibodies are present in the sample, they can bind to the peptide. The bound antibody is then detected by any of a variety of means, which are also well-known in the art. One such detection method employs a second, radiolabelled or enzyme-lablelled antibody which is specific for the Fc region of IgG, for example.

Similarly, measurement of CTL response is well-known.

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One method for measuring CTL response is described in Example II. Thus, peripheral blood cells from a patient can be incubated with peptides of the present invention and together with a target cell population that comprises a fibroblast cell line expresses MHC molecules which present the peptide of the present invention on their surface. Peptide-specific lysis of the target cells is a measure of CTL function in the patient.

The invention being thus described, it will be 10 apparent to one of skill in the art that various modifications of the materials and methods used to make or practice the invention could be employed. Such modifications are to be considered as encompassed by the scope of the invention as claimed below.

REFERENCES

Throughout this paper, reference is made to a number of articles of scientific and patent literature. Each of such papers are hereby incorporated in their entirety by such reference. Those articles which can be found in the technical literature are listed below:

1. Fauci, A. S., Science 239:617 (1988).

2. Lane, H. C., J. M. Depper, W. C. Greene, G. Whalen, T. A. Waldmann, and A. S. Fauci., New Engl. J. Med. 313:79 (1985) .

3. Redfield, R. R. , D. L. Birx, N. Ketter, E.

Tramont, V. Polonis, C. Davis, J. F. Brundage, G. Smith, S. Johnson, A. Fowler, T. Wierzba, A. Shafferman, F. Volvovitz, C. Oster, and D. S. Burke, N. Engl. J. Med. 324:1677 (1991).

4. Schechter, M. T., K. J. P. Craib, T. N. Le, J. S. G. Montaner, B. Douglas, P. Sestak, B. Willoughby, and Μ. V. O'Shaughnessy, AIDS 4:185 (1990).

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<td> 5. Dalgleish, A. Ludlam, B. Gazzard, Lancet 339:824 (1992)</td><td> G. , S. Wilson, M. Gompels, C. A. M. Coates, and J. Habeshaw,</td>

6. Wendler, I., U. Bienzle, and G. Hunsmann, AIDS

Res. Hu. Retroviruses 3:157 (1987).

7. Simmonds, P. , D. Beat son, R. J. G. Cuthbert, H. Watson, B. Reynolds, J. F. Peutherer, J. V. Parry, C. A. Ludlam, and C. M. Steel, Lancet 338:1159 (1991).

8. Nara, P. L. , R. R. Garrity, and J. Goudsmit, FASEB

J. 5:2437 (1991).

9. Nara, P. L. and J. Goudsmit, Clonal dominance of the neutralizing response to the HIV-1 V3 epitope: 'Evidence for original antigenic sin' during vaccination and infection in animals, including humans.

In Vaccines 91. R.M. Channock, H. Ginsberg, F. Brown and R.A. Lerner, eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, p. 37 (1991).

10. Kohler, H. , J. Goudsmit, and P. Nara, J. Acg. Immune Defic. Syndromes 5: 1158 (1992).

11. Robinson, W. E.,Jr., T. Kawamura, M. K. Gorny, D.

Lake, J. -Y. Xu, Y. Matsumoto, T. Sugano, Y. Masuho, W. M. Mitchell, E. Hersh, and S. Zolla-Pazner, Proc. Natl. Acad. Sci. U. S. A. 87:3185 (1990).

12. Habeshaw, J. A., A. G. Dalgleish, L. Bountiff, A.

L. Newell, D. Wilks, L. C. Walker, and F. Manca,

Immunol. Today 21:418 (1990).

13. Takeda, A., C. U. Tuazon, and F. A. Ennis, Science 242:580 (1988).

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14. Robinson, W. E. Jr., D. C. Montefiori, and W. M. Mitchell, Lancet 1988:790 (1988).

15. Berman, P. W. , T. J. Gregory, L. Riddle, G. R. Nakamura, M. A. Champe, J. P. Porter, F. M. Wurm, R. D.

Hershberg, E. K. Cobb, and J. W. Eichberg, Nature 345:622 (1990).

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SEQUENCE

94/26785

PCT/US94/05142

LISTING (1) GENERAL INFORMATION:

(i) APPLICANT:

(A) NAME: Gov't. of the United States as represented by the Department of Health and Human Services/National Institutes of Health (B) (C) (D) (E) (F)

STREET : Box OTT

CITY: Bethesda

STATE OR PROVINCE:Maryland

COUNTRY: United States of America

POSTAL CODE: 20892 (ii) TITLE OF INVENTION: COMPOSITE SYNTHETIC PEPTIDE CONSTRUCT ELICITING NEUTRALIZING ANTIBODIES AND CYTOTOXIC T LYMPHOCYTES AGAINST HIV (iii) NUMBER OF SEQUENCES: 36 (iv) CORRESPONDENCE ADDRESS:

(A) ADDRESSEE: Birch, Stewart, Kolasch & Birch (B) STREET: P.O. Box 747 (C) CITY: Falls Church (D) STATE: Virginia (E) COUNTRY: USA (F) ZIP: 22040-0747 (v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA:

(A) APPLICATION NUMBER: US 08/060,988 (B) FILING DATE: 14-MAY-1993 (C) CLASSIFICATION:

(viii) ATTORNEY/AGENT INFORMATION:

(A) NAME: Svensson, Leonard R.

(B) REGISTRATION NUMBER: 30330 (C) REFERENCE/DOCKET NUMBER: 1173-434P (ix) TELECOMMUNICATION INFORMATION:

(A) TELEPHONE: 703-205-8000 (B) TELEFAX: 703-205-8050 (2) INFORMATION FOR SEQ ID N0:l:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 35 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) HYPOTHETICAL: NO (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide

WO 94/26785

PCT/US94/05142 (B) LOCATION: 1..35 (D) OTHER INFORMATION: /label= peptide /note= pclus l-18/pl8IIIB peptide, see Table 1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:

Ile Gly Lys 35 (2) INFORMATION FOR SEQ ID NO : 2 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 39 amino acids (B) TYPE: amino acid

<td></td><td> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE: (A) NAME/KEY: (B) LOCATION:</td><td> Peptide 1..39</td>

(D) OTHER INFORMATION: /label= peptide /note= pclus 3-18/pl8IIIB peptide, see Table 1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:

Lys Gin Ile Ile Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala 15 10 15

Pro Pro Ile Ser Gly Gin Ile Arg Arg Ile Gin Arg Gly Pro Gly Arg 20 25 30

Ala Phe Val Thr Ile Gly Lys 35 (2) INFORMATION FOR SEQ ID NO:3:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 39 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE :

(A) NAME/KEY: Peptide (B) LOCATION: 1..39 (D) OTHER INFORMATION: /label= peptide /note= pclus 4-18/pl8IIIB peptide, see Table 1 ^ WO 94/26785

PCT/US94/05142 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:

(2) INFORMATION FOR SEQ ID NO :4:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 48 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..48 (D) OTHER INFORMATION: /label= peptide /note» pclus 6-18/pl8IIIB peptide, see Table 1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:

(2) INFORMATION FOR SEQ ID NO:5:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 30 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..30 (D) OTHER INFORMATION: /label» peptide /note» p53/pl8IIIB peptide, see Table 1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 5 :

Asp Arg Val Ile Glu Val Val Gin Gly Ala Tyr Arg Ala Ile Arg Arg 15 10 15

WO 94/26785

PCT/US94/05142

Ile Gin Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 20 25 30 (2) INFORMATION FOR SEQ ID NO : 6 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 31 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..31 (D) OTHER INFORMATION: /label= peptide /note= p55/pl8IIIB peptide, see Table 1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:

Ala Gin Gly Ala Tyr Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg 15 10 15

Arg Ile Gin Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 20 25 30 (2) INFORMATION FOR SEQ ID NO:7:

<i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

<td></td><td> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE : (A) NAME/KEY: (B) LOCATION:</td><td> Peptide 1..15</td>

(D) OTHER INFORMATION: /label= peptide /note= pl8IIIB peptide, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:

Arg Ile Gin Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO:8:

ii) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 13 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal ~W0 94/26785

PCT/US94/05142 (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..13 (D) OTHER INFORMATION: /label= peptide /note= 18RF peptide; peptide homologous to pl8IIIB in RF strain of HIV, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:

Thr Lys Gly Pro Gly Arg Val He Tyr Ala Thr Gly Gin 15 10 (2) INFORMATION FOR SEQ ID NO : 9 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 14 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..14 (D) OTHER INFORMATION: /label= peptide /note= plS-l, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:

He Gin Arg Gly Pro Gly Arg Ala Phe Val Thr He Gly Lys 15 10 (2) INFORMATION FOR SEQ ID NO:10:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 14 amino acids (B) TYPE: amino acid

<td></td><td colspan="2"> (D) TOPOLOGY: linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE:</td><td></td>

(A) NAME/KEY: Peptide (B) LOCATION: 1..14 (D) OTHER INFORMATION: /label= peptide /note= pl8-2, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:

Arg Gin Arg Gly Pro Gly Arg Ala Phe Val Thr He Gly Lys 15 10 (2) INFORMATION FOR SEQ ID NO :11:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear

WO 94/26785

PCT/US94/05142 (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= pl8-3, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:

Arg Ile Thr Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO:12:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE : internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= pl8-4, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:

Arg Ile Gin Lys Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO:13:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

<td></td><td> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE:</td><td></td>

<td></td><td> (A) NAME/KEY:</td><td> Peptide</td>

<td></td><td> (B) LOCATION:</td><td> 1..15</td>

(D) OTHER INFORMATION: /label= peptide /note= pl8-5, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:

Arg Ile Gin Arg Ala Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 15 io 15

WO 94/26785

PCT/US94/05142 (2) INFORMATION FOR SEQ ID NO :14:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

<td colspan="2"> (D) TOPOLOGY: linear</td>

<td> (ii) MOLECULE TYPE:</td><td> peptide</td>

<td> (v) FRAGMENT TYPE:</td><td> internal</td>

<td> (ix) FEATURE : (A) NAME/KEY: (B) LOCATION:</td><td> Peptide 1..15</td>

(D) OTHER INFORMATION: /label= peptide /note= p!8-6, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:

Arg lie Gin Arg Gly Ala Gly Arg Ala Phe Val Thr lie Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO:15:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

<td></td><td> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE:</td><td></td>

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= '·ρ18-7, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:

Arg lie Gin Arg Gly Pro Ala Arg Ala Phe Val Thr lie Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO:16:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

<td></td><td colspan="2"> (D) TOPOLOGY: linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE:</td><td></td>

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= pl8-8, see Table V

WO 94/26785

PCT/US94/O5142 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:

Arg Ile Gin Arg Gly Pro Gly Ala Ala Phe Val Thr Ile Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO :17:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

<td></td><td> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (V)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE: (A) NAME/KEY: (B) LOCATION:</td><td> Peptide 1..15</td>

(D) OTHER INFORMATION: /label= peptide /note= pl8-9, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:

Arg Ile Gin Arg Gly Pro Gly Arg Val Phe Val Thr lie Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO :18:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

<td></td><td colspan="2"> (D) TOPOLOGY: linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE :</td><td></td>

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= pl8-l0, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO :18:

Arg Ile Gin Arg Gly Pro Gly Arg Ala Ile Val Thr Ile Gly Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO:19:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal — WO 94/26785

PCT/US94/05142 (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= p!8-ll, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:

Arg lie Gin Arg Gly Pro Gly Arg Ala Phe Tyr Thr 15 10 (2) INFORMATION FOR SEQ ID NO :20:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

He Gly Lys 15

<td></td><td> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE : (A) NAME/KEY: (B) LOCATION:</td><td> Peptide 1..15</td>

(D) OTHER INFORMATION: /label= peptide /note= pl8-12, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:

Arg He Gin Arg Gly Pro Gly Arg Ala Phe Val Ala 15 10 (2) INFORMATION FOR SEQ ID NO :21:

(i). SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid

He Gly Lys 15

<td></td><td> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE: (A) NAME/KEY: (B) LOCATION:</td><td> Peptide 1..15</td>

(D) OTHER INFORMATION: /label= peptide /note= pl8-13, see Table V’1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:

Arg He Gin Arg Gly Pro Gly Arg Ala Phe Val Thr 15 10

Thr Gly Lys 15 (2) INFORMATION FOR SEQ ID NO:22:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal

WO 94/26785

PCT/US94/05142 (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= pl8-14, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO :22:

Arg lie Gin Arg Gly Pro Gly Arg Ala Phe Val Thr lie Ala Lys 15 10 15 (2) INFORMATION FOR SEQ ID NO:23:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= pl8-15, see Table V (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:

Arg lie Gin Arg Gly Pro Gly Arg Ala Phe Val Thr lie Gly Gin 15 10 15 (2) INFORMATION FOR SEQ ID NO:24:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 24 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..24 (D) OTHER INFORMATION: /label= peptide /note= pclus3 peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24:

Lys Gin lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala 15 10 15

Pro Pro lie Ser Gly Gin lie Arg 20

WO 94/26785

PCT/US94/05142 (2) INFORMATION FOR SEQ ID NO :25:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 25 amino acids (B) TYPE: amino acid

<td></td><td> (D) TOPOLOGY:</td><td> linear</td><td></td><td></td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td><td></td><td></td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td><td></td><td></td>

<td> (ix)</td><td> FEATURE :</td><td></td><td></td><td></td>

<td></td><td> (A) NAME/KEY:</td><td> Peptide</td><td></td><td></td>

<td></td><td> (B) LOCATION:</td><td> 1..25</td><td></td><td></td>

<td></td><td colspan="2"> (D) OTHER INFORMATION:</td><td> /label» peptide</td><td></td>

<td></td><td> /note»</td><td colspan="2"> pclus4 peptide</td><td></td>

<td> (xi)</td><td colspan="3"> SEQUENCE DESCRIPTION: SEQ ID NO:25:</td><td></td>

25 (2) INFORMATION FOR SEQ ID NO:26:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 33 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..33 (D) OTHER INFORMATION: /label» peptide /note» pclus6 peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:

Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val Val Gin Gly Ala

5 10 15

Tyr Arg Ala lie Arg His Ile Pro Arg Arg Ile Arg Gin Gly Leu Glu

25 30

Arg (2) INFORMATION FOR SEQ ID NO:27:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 35 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal

WO 94/26785

PCT/US94/05142 (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..35 (D) OTHER INFORMATION: /label= peptide /note= pclusl-18MN peptide, see Table VI (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27:

<td colspan="2"> INFORMATION FOR SEQ ID NO :28:</td>

<td> (i)</td><td> SEQUENCE CHARACTERISTICS: (A) LENGTH: 39 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear</td>

<td> (ii)</td><td> MOLECULE TYPE: peptide</td>

<td> (v)</td><td> FRAGMENT TYPE: internal</td>

(ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..39 (D) OTHER INFORMATION: /label= peptide /note= pclus3-18MN peptide, see Table VI (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28:

(2) INFORMATION FOR SEQ ID NO :29:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 39 amino acids (B) TYPE: amino acid

<td></td><td colspan="2"> (D) TOPOLOGY:</td><td> linear</td>

<td> (ii)</td><td> MOLECULE</td><td> TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT</td><td> TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE :</td><td></td><td></td>

(A) NAME/KEY: Peptide (B) LOCATION: 1..39 (D) OTHER INFORMATION: /label= peptide /note= pclus4-18MN peptide, see Table VI ^WO 94/26785

PCT/US94/05142 (xi) SEQUENCE DESCRIPTION: SEQ ID NO :29:

(2) INFORMATION FOR SEQ ID NO:30:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 48 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..48 (D) OTHER INFORMATION: /label= peptide /note= pclus6-18MN peptide, see Table VI (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:

(2) INFORMATION FOR SEQ ID NO:31:

<i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 42 amino acids (B) TYPE: amino acid

<td></td><td colspan="2"> (D) TOPOLOGY: linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE :</td><td></td>

(A) NAME/KEY: Peptide (B) LOCATION: 1..42 (D) OTHER INFORMATION: /label= peptide /note= pclus6.1-18MN peptide, see Table VI (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:

Asp Arg Val lie Glu Val Val Gin Gly Ala Tyr Arg Ala lie Arg His 15 10 15

WO 94/26785

PCT/US94/05142

40 (2) INFORMATION FOR SEQ ID NO:32:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 30 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..30 (D) OTHER INFORMATION: /label= peptide /note= p53-18MN peptide, see Table VI (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32:

(2) INFORMATION FOR SEQ ID NO:33:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 31 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..31 (D) OTHER INFORMATION: /label= peptide /note= p55-18MN peptide, see Table VI (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:

(2) INFORMATION FOR SEQ ID NO :34:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 15 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear — WO 94/26785 “J ^2880 PCT/US94/05142 (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..15 (D) OTHER INFORMATION: /label= peptide /note= pl8MN peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34:

Arg He His He Gly Pro Gly Arg Ala Phe Tyr Thr Thr Lys Asn 1 5 10 '15 (2) INFORMATION FOR SEQ ID NO :35:

ii) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 66 amino acids (B) TYPE: amino acid

<td></td><td colspan="2"> (D) TOPOLOGY: linear</td>

<td> (ii)</td><td> MOLECULE TYPE:</td><td> peptide</td>

<td> (v)</td><td> FRAGMENT TYPE:</td><td> internal</td>

<td> (ix)</td><td> FEATURE:</td><td></td>

(A) NAME/KEY: Peptide (B) LOCATION: 1..66 (D) OTHER INFORMATION: /label= peptide /note= peptide from P. falciparum CS antigen (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35:

Cys Ser 65 (2) INFORMATION FOR SEQ ID NO:36:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 42 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal

WO 94/26785 (ix) FEATURE:

(A) NAME/KEY: Peptide (B) LOCATION: 1..42 (D) OTHER INFORMATION: /label= peptide /note=pclus6.1-18IIIB peptide

Asp Arg Val Ile Glu Val Val Gin Gly Ala Tyr Arg Ala Ile Arg His 15 10 15

Ile Pro Arg Arg Ile Arg Gin Gly Leu Glu Arg Arg Ile Gin Arg Gly 20 25 30

PCT/US94/05142

Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 35 40

Contents166

100 members in 12 offices

This recordMember, by publication datePriority claim2 more offices are in the list below

Priority claims9

Priority claims
Document	Office	Kind	Date
08060988	United States of America	–	1993-05-14
6098893	United States of America	A	1993-05-14
6098893	United States of America	A	1993-05-14
9405142	United States of America	W	1994-05-13
9405142	United States of America	W	1994-05-13
08060988	–	–	–
PCTUS94005142	–	–	–
US19930060988	–	–	–
WO1994US05142	–	–	–

Members100

Family members, oldest first
Document	Office	Kind
US4621006A	United States of America	A	1986-11-04
IL84980D0	Israel	D0	1988-06-30
EP0273716A2	European Patent Office (EPO)	A2	1988-07-06
WO8805051A1	World Intellectual Property Organization (WIPO)	A1	1988-07-14
AU1365788A	Australia	A	1988-07-27
JPS63503227A	Japan	A	1988-11-24
US4853065A	United States of America	A	1989-08-01
WO8907112A1	World Intellectual Property Organization (WIPO)	A1	1989-08-10
AU3204489A	Australia	A	1989-08-25
EP0273716A3	European Patent Office (EPO)	A3	1989-12-20
AU592258B2	Australia	B2	1990-01-04
WO9000901A1	World Intellectual Property Organization (WIPO)	A1	1990-02-08
IL91007D0	Israel	D0	1990-02-09
AU3967089A	Australia	A	1990-02-19
EP0400076A1	European Patent Office (EPO)	A1	1990-12-05
US5021282A	United States of America	A	1991-06-04
EP0429477A1	European Patent Office (EPO)	A1	1991-06-05
US5030449A	United States of America	A	1991-07-09
JPH03503166A	Japan	A	1991-07-18
JPH03504384A	Japan	A	1991-09-26
EP0400076A4	European Patent Office (EPO)	A4	1991-11-13
EP0429477A4	European Patent Office (EPO)	A4	1991-11-13
US5081226A	United States of America	A	1992-01-14
AU621097B2	Australia	B2	1992-03-05
AU624628B2	Australia	B2	1992-06-18
US5154963A	United States of America	A	1992-10-13
CA2114849A1	Canada	A1	1993-03-18
WO9304697A1	World Intellectual Property Organization (WIPO)	A1	1993-03-18
WO9305812A1	World Intellectual Property Organization (WIPO)	A1	1993-04-01
AU2569392A	Australia	A	1993-04-05
AU2571992A	Australia	A	1993-04-27
IL84980A	Israel	A	1993-06-10
IL89012A	Israel	A	1993-07-08
EP0273716B1	European Patent Office (EPO)	B1	1993-08-11
AT92934T	Austria	T	1993-08-15
ATE92934T1	Austria	T1	1993-08-15
CA2131153A1	Canada	A1	1993-09-16
DE3787002D1	Germany	D1	1993-09-16
WO9318055A2	World Intellectual Property Organization (WIPO)	A2	1993-09-16
AU3787893A	Australia	A	1993-10-05
DE3787002T2	Germany	T2	1993-11-25
WO9318055A3	World Intellectual Property Organization (WIPO)	A3	1994-03-17
EP0601108A1	European Patent Office (EPO)	A1	1994-06-15
CA2162880A1	Canada	A1	1994-11-24
WO9426785A1	World Intellectual Property Organization (WIPO)	A1	1994-11-24
AU7017394A	Australia	A	1994-12-12
EP0630385A1	European Patent Office (EPO)	A1	1994-12-28
JPH07501516A	Japan	A	1995-02-16
JPH0762031B2	Japan	B2	1995-07-05
IL91007A	Israel	A	1995-08-31
AU665023B2	Australia	B2	1995-12-14
US5500269A	United States of America	A	1996-03-19
EP0701572A1	European Patent Office (EPO)	A1	1996-03-20
EP0400076B1	European Patent Office (EPO)	B1	1996-05-15
AU668927B2	Australia	B2	1996-05-23
AT138077T	Austria	T	1996-06-15
ATE138077T1	Austria	T1	1996-06-15
DE68926499D1	Germany	D1	1996-06-20
EP0429477B1	European Patent Office (EPO)	B1	1996-10-16
AT144143T	Austria	T	1996-11-15
ATE144143T1	Austria	T1	1996-11-15
DE68927348D1	Germany	D1	1996-11-21
DE68926499T2	Germany	T2	1996-12-19
JP2569185B2	Japan	B2	1997-01-08
JPH09500614A	Japan	A	1997-01-21
DE68927348T2	Germany	T2	1997-02-13
EP0601108A4	European Patent Office (EPO)	A4	1997-03-19
CA1339240C	Canada	C	1997-08-05
JP2676166B2	Japan	B2	1997-11-12
US5711947A	United States of America	A	1998-01-27
AU688333B2	Australia	B2	1998-03-12
US5820865A	United States of America	A	1998-10-13
US5932218A	United States of America	A	1999-08-03
EP0701572B1	European Patent Office (EPO)	B1	1999-08-11
AT183200T	Austria	T	1999-08-15
ATE183200T1	Austria	T1	1999-08-15
US5939074A	United States of America	A	1999-08-17
DE69420034D1	Germany	D1	1999-09-16
EP0601108B1	European Patent Office (EPO)	B1	1999-10-06
AT185275T	Austria	T	1999-10-15
ATE185275T1	Austria	T1	1999-10-15
US5976541A	United States of America	A	1999-11-02
DE69230106D1	Germany	D1	1999-11-11
ES2138086T3	Spain	T3	2000-01-01
CA1340896C	Canada	C	2000-02-15
CA1340907C	Canada	C	2000-02-22
GR3031790T3	Greece	T3	2000-02-29
DE69420034T2	Germany	T2	2000-03-02
DK0701572T3	Denmark	T3	2000-03-13
DE69230106T2	Germany	T2	2000-03-30
DK0601108T3	Denmark	T3	2000-04-17
US6214347B1	United States of America	B1	2001-04-10
US6294322B1	United States of America	B1	2001-09-25
CA2162880CThis record	Canada	C	2003-02-11
JP2006056893A	Japan	A	2006-03-02
JP3802049B2	Japan	B2	2006-07-26
ES2257733T3	Spain	T3	2006-08-01
US7094405B1	United States of America	B1	2006-08-22
CA2114849C	Canada	C	2007-04-24
CA2131153C	Canada	C	2008-02-12

2 legal events, as the office reported them to INPADOC

Over the term

Point at a mark for the event

Events

Legal events, newest first
	Event	Code
2013-06-24	LapsedLapsedMKLA	MKLA
1996-05-30	Examination requestEEER	EEER