Optically pure salts of pyridinylmethyl sulfinyl-ih- benzimidazole compounds

PCT/SE94/00509

WO 94/27988

Optically pure salts of pyridinylmethyl sulfinyl-IHbenzimidazole compounds.

Field of the invention

The present invention is directed to new compounds with high optical purity, their use in medicine, a process for their preparation and their use in the manufacture of pharmaceutical preparation. The invention also relates to novel intermediates in the preparation of the compounds of the invention.

Background of the invention

The compound 5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2pyridinyl)methyl] sulfinyl]-IH-benzimidazole, having the generic name omeprazole, and therapeutically acceptable alkaline salts thereof are described in EP 5129 and

EP 124 495, respectively. Omeprazole and its alkaline salts are effective gastric acid secretion inhibitors, and are useful as antiulcer agents. The compounds, being sulfoxides, have an asymmetric center in the sulfur atom, i.e. exist as two optical isomers (enantiomers). It is desirable to obtain compounds with improved pharmacokinetic and metabolic properties which will give an improved therapeutic profile such as a lower degree of interindividual variation. The present invention provides such compounds, which are novel salts of single enantiomers of omeprazole.

The separation of the enantiomers of omeprazole in analytical scale is described in

e.g. J. Chromatography, 532 (1990), 305-19 and in a preparative scale in DE 4035455. The latter has been done by using a diastereomeric ether which is separated and thereafter hydrolysed in an acidic solution. Under the acidic conditions needed for hydrolysis of the attached group, omeprazole is quite sensitive and the acid has to be quickly neutralized with a base to avoid degradation of the acid-sensitive compound. In the above mentioned application

WO 94/27988

PCT/SE94/00509 this is done by adding the reaction mixture containing concentrated sulfuric acid to a concentrated solution of NaOH. This is disadvantageous because there is a great risk of locally reaching pH values between Î-6, which would be devastating for the substance. Moreover, instantaneous neutralisation will create heat which will be difficult to handle in large scale production.

The present invention in a further aspect provides a novel method for preparing the novel compounds of the invention in large scale. This novel method can also be used in large scale to obtain single enantiomers of omeprazole in neutral form.

There is no example known in the prior art of any isolated or characterized salt of optically pure omeprazole, i.e. single enantiomers of omeprazole neither of any isolated or characterized salt of any optically pure omeprazole analogue.

Detailed description of the invention

The present invention refers to the new Na⁺, Mg^⁺, Li⁺, K⁺, Ca^⁺ and N⁺(R)^ salts of the single enantiomers of omeprazole, where R is an alkyl with 1-4 carbon atoms, i.e. Na⁺, Mg^⁺, Li⁺, K⁺, Ca^⁺ and N^CR)^ salts of (+)-5-methoxy-2-[[(420 methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-lH-benzimidazole and (-)-5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfmyl]-lHbenzimidazole, where R is an alkyl with 1-4 carbon atoms.

Particularly preferred salts according to the invention are the Na⁺, Ca^⁺ and Mg^⁺ salts, i.e (+)-5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]lH-benzimidazole sodium salt, (-)-5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2pyridinyl)methyl] sulfinyl]-lH-benzimidazole sodium salt, (+)-5-methoxy-2-[[(4methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-lH-benzimidazole magnesium salt, (-)-5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-lH30 benzimidazole magnesium salt, (+)-5-methoxy-2-[[(4-methoxy-3,5-dimethyl-22135653

WO 94/27988

PCT7SE94/00509 pyridinyl)methyl]sulfinyl]-lH-benzimidazoie calcium salt and (-)-5-methoxy-2-[[(4methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-lH-benzimidazole calcium salt

Most preferred salts according to the invention are the optically pure Na⁺ salts of 5 omeprazole according to compounds la and lb

<img file="CA2139653C_D0001.tif" />

la (+)-enantiomer lb (-)-enantiomer and the optically pure magnesium salts of omeprazole according to compounds Ha and lib

<img file="CA2139653C_D0002.tif" />

Ila (+)-enantiomer lib (-)-enantiomer

CA 02139653 1999-11-02

With the expression optically pure Na⁺ salts of omeprazole is meant the (+)-enantiomer of omeprazole Na-salt essentially free of the (-)-enantiomer of omeprazole Na-salt and the (-)enantiomer essentially free of the (+)-enantiomer, respectively. Single enantiomers of omeprazole have hitherto only been obtained as syrups and not as crystalline products.

By means of the novel specific method according to one aspect of the invention by preparing the single enantiomers of omeprazole, the salts defined by the present invention are easy to obtain. In addition, the salts, however not the neutral forms, are obtained as crystalline products. Because it is possible to purify optically impure salts of the enantiomers of omeprazole by crystallisation, they can be obtained in very high optical purity, in some instances >99.8% enantiomeric excess (e.e.) even from an optically contaminated preparation. Moreover, the optically pure salts are stable towards racemization both in neutral pH and basic pH, which was surprising since the known deprotonation at the carbon atom between the pyridine ring and the chiral sulphur atom was expected to cause racemization under alkaline conditions. This high stability towards racemization makes it possible to use a single enantiomeric salt of the invention in therapy.

The specific method of preparation of the single enantiomers of omeprazole is a further aspect of the invention as mentioned above and it can be used to obtain the single enantiomers of omeprazole in neutral from as well as the salts thereof.

The compounds according to the invention may be used for inhibiting gastric acid secretion in mammals and man. In a more general sense, the compounds of the invention may be used for the treatment of gastric acid-related diseases and gastrointestinal inflammatory diseases in mammals and man, such as gastric ulcer, duodenal ulcer, reflux esophagitis and gastritis. Furthermore, the compounds may be used for

CA 02139653 1999-11-02

4a treatment of other gastrointestinal disorders where gastric antisecretory effect is desirable e.g. in patients on NSAID therapy, in patients with gastrinomas, and in patients with acute upper gastrointestinal bleeding. They may also be used

PCT/SE94/00509

WO 94/27988 in patients in intensive care situations, and pre- and postoperatively to prevent acid aspiration and stress ulceration. The compound of the invention may also be used for treatment or prophylaxis of inflammatory conditions in mammals, including man, especially those involving lysozymal enzymes. Conditions that may be specifically mentioned are rheumatoid arthritis and gout. The compound of the invention may also be useful in the treatment of psoriasis as well as in the treatment of Helicobacter infections.

Yet a further aspect of the invention is the compound III, which is an intermediate 10 used in the specific method of preparation.

och₃

<img file="CA2139653C_D0003.tif" />

Preparation

The optically pure compounds of the invention, i.e. the single enantiomers, are 25 prepared by separating the two stereoisomers of a diastereomeric mixture of the following type, 5- or 6-methoxy-2-[[(4-methoxy-3,5-dimethyl-2pyridinyl)methyl]sulfinyl]-l-[acyloxymethyl]-lH-benzimidazole, formula IV

CA 02139653 1999-11-02

<img file="CA2139653C_D0004.tif" />

ch₃

<img file="CA2139653C_D0005.tif" />

(IV) wherein the methoxy substituent in the benzimidazole moiety is in position 5 or 6, and wherein the Acyl radical is as defined below, followed by a solvolysis of each separated diastereomer in an alkaline solution. The formed single enantiomers of omeprazole are then isolated by neutralizing aqueous solutions of the salts of the single enantiomers of omeprazole with a neutralizing agent which can be an acid or an ester such as methyl formate.

The Acyl moiety in the diastereomeric ester may be a chiral acyl group such as mandeloyl, and the asymmetric center in the chiral acyl group can have either R or S configuration.

The diastereomeric esters can be separated either by chromatography or fractional crystallization.

The solvolysis usually takes place together with a base in a protic solvent such as alcohols or water, but the acyl group may also be hydrolysed off by a base in an aprotic solvent such as dimethyisulfoxide or drniethylformamide. The reacting base may be OH* or R^O where R^ can be any alkyl or aryl group.

To obtain the optically pure Na⁺ salts of the invention,

i.e. the single enantiomers of omeprazole Na⁺ salts, the compound resulting from the solvolysis is treated with a base, such as

WO 94/27988 £139653

PCT/SE94/00509

7

NaOH, in an aqueous or nonaqueous medium, or with NaOR wherein R is an alkyl group containing 1-4 carbon atoms, or with NaNF^· Also alkaline salts wherein the cation is Li⁺ or K⁺ may be prepared using lithium or potassium salts of the above mentioned bases. In order to obtain the crystalline form of the Na⁺ salt, addition of NaOH in a non-aqueous medium such as a mixture of 2-butanone and toluene, is preferred.

To obtain the optically pure Mg^⁺ salts of the invention, optically pure Na⁺ salts are treated with an aqueous solution of an inorganic magnesium salt such as

MgC^, whereupon the Mg^⁺ salts are precipitated. The optically pure Mg^⁺ salts may also be prepared by treating single enantiomers of omeprazole with a base, such as Mg(OR^J>2, wherein R is an alkyl group containing 1-4 carbon atoms, in a non-aqueous solvent such as alcohol (only for alcoholates), e.g. ROH, or in an ether such as tetrahydrofuran. In an analogous way, also alkaline salts wherein the cation is Ca^⁺ can be prepared, using an aqueous solution of an inorganic calcium salt such as CaC^.

Alkaline salts of the single enantiomers of the invention are, as mentioned above, beside the sodium salts (compounds la and lb) and the magnesium salts (compound

Ila and lib), exemplified by their salts with Li⁺, K⁺, Ca^⁺ and N+iR^, where R is an alkyl with 1-4 C-atoms.

For clinical use the single enantiomers, i.e. the optically pure compounds, of the invention are formulated into pharmaceutical formulations for oral, rectal, parenteral ot other modes of administrations. The pharmaceutical formulations contain the single enantiomers of the invention normally in combination with a pharmaceutically acceptable carrier. The carrier may be in form of a solid, semisolid or liquid diluent, or capsule. These pharmaceutical preparations are a further object of the invention. Usually the amount of active compound is between 0.130 95% by weight of the preparation, between 0.2-20% by weight in preparations for

WO 94/27988

PCT/SE94/00509 parenteral use and between 1-50% by weight in preparations for oral administration.

In the preparation of pharmaceutical formulations in form of dosage units for oral 5 administration the optically pure compound may be mixed with a solid, powdered carrier, such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose dérivâtes, gelatin or another suitable carrier, stabilizing substances such as alkaline compounds e.g. carbonates, hydroxides and oxides of sodium, potassium, calcium, magnesium and the like as well as with lubricating agents such as magnesium stearate, calcium stearate, sodium stearyl fumarate and polyethylenglycol waxes. The mixture is then processed into granules or pressed into tablets. Granules and tablets may be coated with an enteric coating which protects the active compound from acid catalysed degradation as long as the dosage form remains in the stomach. The enteric coating is chosen among pharmaceutically acceptable enteric-coating materials e.g. beeswax, shellac or anionic film-forming polymers and the like, if preferred in combination with a suitable plasticizer. To the coating various dyes may be added in order to distinguish among tablets or granules with different amounts of the active compound present

Soft gelatine capsules may be prepared with capsules containing a mixture of the active compound, vegetable oil, fat, or other suitable vehicle for soft gelatine capsules. Soft gelatine capsules may also be enteric-coated as described above.

Hard gelatine capsules may contain granules or enteric-coated granules of the active compound. Hard gelatine capsules may also contain the active compound in combination with a solid powdered carrier such as lactose, saccharose, sorbitol, mannitol, potato starch, amylopectin, cellulose dérivâtes or gelatin. The capsules may be enteric-coated as described above.

WO 94/27988

2139653 PCT/SE94/00509

Dosage units for rectal administration may be prepared in the form of suppositories which contain the active substance mixed with a neutral fat base, or they may be prepared in the form of a gelatine rectal capsule which contains the active substance in a mixture with a vegetable oil, paraffin oil or other suitable vehicle for gelatine rectal capsules, or they may be prepared in the form of a ready-made micro enema, or they may be prepared in the form of a dry micro enema formulation to be reconstituted in a suitable solvent just prior to administration.

Liquid preparation for oral administration may be prepared in the form of syrups or 10 suspensions, e.g. solutions or suspensions containing from 0.2% to 20% by weight of the active ingredient and the remainder consisting of sugar or sugar alcohols and a mixture of ethanol, water, glycerol, propylene glycol and/or polyethylene glycol. If desired, such liquid preparations may contain colouring agents, flavouring agents, saccharine and carboxymethyl cellulose or other thickening agents. Liquid preparations for oral administration may also be prepared in the form of dry powder to be reconstituted with a suitable solvent prior to use.

Solutions for parenteral administrations may be prepared as solutions of the optically pure compounds of the invention in pharmaceutically acceptable solvents, preferably in a concentration from 0.1 to 10% by weight These soultions may also contain stabilizing agents and/or buffering agents and may be manufactured in different unit dose ampoules or vials. Solutions for parenteral administration may also be prepared as dry preparations to be reconstituted with a suitable solvent extemporaneously before use.

The typical daily dose of the active compound will depend on various factors such as for example the individual requirement of each patient, the route of administration and the disease. In general, oral and parenteral dosages will be in the range of 5 to 500 mg per day of active substance.

CA 02139653 1999-11-02

9a

The invention is illustrated by the following examples. It will be noted from the examples that the preparation of the optically pure salts of omeprazole will result in a change of direction from (-) to (+) optical rotation when preparing the sodium salt from the non-salt form and vice versa from (+) to (-) optical rotation when preparing the magnesium salt from the sodium salt.

CA 02139653 1999-11-02

Example 1. Preparation of (+)-5-methoxy-2-fr(4-mcthoxy-3,5-dimeAyl-2pvridinvDmethyllsulfinvil-IH-bcnzimidazole sodium salt

100 mg (0.3 mmol) of G)-5-methoxy-2-([(4-methoxy-3^-dimethyi-2-pyridinyl)methyl]suffinyl]-l H-benzimidazole (contaminated with 3% of the (+)-isomer) was dissolved in 1 ml of 2-butanone with stirring. 60 μΐ of an aqueous solution of 5.0 M sodium hydroxide and 2 ml of toluene were added. The resultant mixture was non-homogeneous. In order to obtain a clear solution, more 2-butanone was added (ca 1 ml) and the mixture was stirred at ambient temperature over nightThe formed precipitate was filtered off and washed with ether. There was obtained 51 mg (46%) of the title compound as white crystals m.p. (decomposition) 246-248°C. The optical purity (e.e.) which was analyzed by chiral column chromatography was 299.8%. [a]^°= +42,8° (c=0_5%, water).

NMR data are given below.

Example 2. Preparation of (->-5-methoxy-2-rf(4-methoxy-3_T5-dimethyl-2pyridinyl)methvl1suIfinyll-lH-benzimidazolc sodium salt

100 mg (0.3 mmol) of (+)-5-methoxy-2-[[(4-methoxy-3_r5-dimethyI-2-pyridinyl)methyI]sulfinyl]-lH.-benzimidazole (contaminated with 3% of the (-)-isomer) was dissolved in 1 ml of 2-butanone with stirring. 60 pi of an aqueous solution of 5.0 M sodium hydroxide and 2 ml of toluene were added. The resultant mixture was non-homogeneous. In order to obtain a clear solution, more 2-butanone was added (ca 1 ml) and the mixture was stirred at ambient temperature over night The formed precipitate was filtered off and washed with ether. There was obtained 56 mg (51%) of the title compound as white crystals m.p. (decomposition) 247-249°C.

WO 94/27988 , μ SS ¢5 3

PCT/SE94/00509

The optical purity (e.e.) which was analyzed by chiral column chromatography was >99.8%. [α]θθ= -44.Γ (c=0.5%, water).

NMR data are given below.

Example 3. Preparation of (+)-5-methoxy-2-IT(4-methoxy-3,5-dirnethvl-2pyridinvDmethyllsulfinvIl-IH-benzimidazole magnesium salt

2.9 ml of a 0.1 M solution of NaOH was added to 0.10 g (0.29 mmol) (+)-510 methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]- 1Hbenzimidazole. To this mixture 2 ml methylene chloride was added, and after mixing in a separatory funnel the aqueous solution was separated off. A solution of 14 mg (0.145 mmol) MgC^ in water was added dropwise. The formed precipitate was isolated by centrifugation, and 52 mg (50%) of the product was isolated as an amorphous powder. The optical purity (e.e.) was 98%, and thus the same as the starting material. The optical purity was determined by 20 chromatography on an analytical chiral column. Mp = +101.2° (c=l%, methanol). The Mg content of the sample was found to be 3.0%, shown by atomic absorption spectroscopy.

Example 4, Preparation of (+)-5-methoxy-2-fi(4-methoxy-3,5-dimethyl-2pyridinyl)methyI1sulfinyll-lH-benzimidazole magnesium salt (-)-5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-lH25 benzimidazole sodium salt (0.500 g, 1.36 mmol) was dissolved in water (10 ml).

To this mixture 10 ml of an aqueous solution of MgCljxHjO (138 mg, 0.68 mmol) was added dropwise and the formed precipitate was isolated by centrifugation. There was obtained 418 mg (86%) of the product as a white powder. The optical purity (ee) of the product was 99.8% which was the same as the optical purity of the starting material. The optical purity was determined by

WO 94/27988

PCT/SE94/00509

0 chromatography on an analytical chiral column. [a]^ = +129.9° (c=l%, methanol).

Example 5. Preparation of (-)-5-methoxy-2-[[(4-metboxy-3,5-dimethyl-2pyridinyllmethyllsulfinyll-lH-benzimidazoie magnesium salt (+)-5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]-sulfinyl]-lHbenzimidazole sodium salt (0.165 g, 0.45 mmol) was dissolved in water (3 ml). To this mixture 2 ml of an aqueous solution of MgC^xH^O (46 mg, 0.23 mmol) was added dropwise and the formed precipitate was isolated by centrifugation. There was obtained 85 mg (51%) of the product as a white powder. The optical purity (ee) of the product was 99.9% which was the same or better as the optical purity of the starting material. The optical purity was determined by chromatography on an analytical chiral column. [a]p = -128.2° (c=l%, methanol).

Table 1

Ex. Solvent

1. DMSO-dg 500 MHz

NMR data δ ppm

2.20 (s, 3H), 2.22 (s, 3H), 3.69 (s, 3H), 3.72 (s, 3H), 4.37 (d, 1H), 4.75 (d, 1H), 6.54 (dd, 1H), 6.96 (d, 1H) 7.30 (d, 1H), 8.21 (s, 1H).

2.

DMSO-dg 2.20 (s, 3H), 2.22 (s, 3H), 3.69 (s, 3H), 3.72 (s, 3H),

500 MHz 4.38 (d, 1H), 4.73 (d, 1H), 6.54 (dd, 1H), 6.96 (d, 1H), 7.31 (d, 1H), 8.21 (s, 1H).

Preparation of the synthetic intermediates according to the invention will be described in the following examples.

WO 94/27988

PCT/SE94/00509

Example 6. Preparation of 6-methoxv-2-ri(4-methoxy-3,5-dimethyl-2pyridinvI)methyn-(R/S)-sulfinyl1-l-f(R)-mandelovloxymethyl1-lH-benzimidazole

A solution of 3.4 g sodium hydroxide in 40 ml water was added to a mixture of 5 14.4 g (42 mmol) tetrabutylammonium hydrogen sulphate and 6.4 g (42 mmol) (R)-(-)-mandelic acid. The mixture was extracted with 400 ml chloroform. After separation, the organic extract was heated to reflux with 16.6 g (42 mmol) of the racemate of 6-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]-sulfinyl]l-[chloromethyl]-lH-benzimidazole. Evaporation of the solvent was followed by dilution with 100 ml dichloromethane and 700 ml ethyl acetate. The mixture was washed with 3 x 200 ml water and the organic solution was dried over MgSO^ and then evaporated. The crude material was purified by recrystallization from 100 ml acetonitrile, giving 8.1 g of the title compound (38%) as a diastereomeric mixture.

NMR data are given below.

Example 7. Separation of the more hydrophilic diastereomer of 6-methoxy-2-IT(4methoxv^^-dimethvl^-pyridinyDmethyll-iR/SI-sulfmvn-l-rfR)mandelovloxymethvlI-lH-benzimidazole

The diastereomers of the title compound in Example 6 were separated using reversed phase chromatography (HPLC). Approximately 300 mg of the diastereomeric mixture was dissolved in 10 ml hot acetonitrile which was diluted with 10 ml of a mixture of aqueous 0.1 M ammoniumacetate and acetonitrile (70/30). The solution was injected to the column and the compounds were eluted with a mixture of aqueous 0.1 M ammoniumacetate and acetonitrile (70/30). The more hydrophilic isomer was easier to obtain pure than the less hydrophilic one. The work up procedure for the fraction which contained pure isomer was as follows; extraction with dichloromethane, washing the organic solution with aqueous 5 % sodium hydrogen carbonate solution, drying over Na2SO^ and

WO 94/27988

PCT/SE94/00509

<img file="CA2139653C_D0006.tif" />

evaporation of the solvent on a rotavapor (at the end of the evaporation the removal of acetonitrile was facilitated by adding more dichloromethane). Using 1.2 g of the diastereomeric mixture with the above mentioned technique, the more hydrophilic isomer, 410 mg, was obtained in a pure state as a colourless syrup.

NMR data are given below.

Example 8. Preparation of 6-methoxv-2-[i(4-methoxv-3,5-dimethyl-2Pvridinvl)methvn-(R/S)-sulfinvn-l-i(S)-mandelovloxvmethvl1-lH-benzimidazole

The product was obtained from 8.1 g (202 mmol) sodium hydroxide in 100 ml water, 34.4 g (101 mmol) tetrabutylammonium hydrogen sulfate, 15.4 g (101 mmol) (S)-(+)-mandelic acid and 39.9 g (101 mmol) of the racemate of 6-methoxy 2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]-sulfmyl]-1 -[chloromethyl]-1H15 benzimidazole using the same procedure as in Example 6. Recrystallization from 100 ml acetonitrile yielded 21.3 g, i.e. 41% of the title compound as a diastereomeric mixture.

NMR data are given below.

Example 9. Separation of the more hydrophilic diastereomer of 6-methoxy-2-[[(4methoxv-3,5-dimethvl-2-pvridinvl)methvl1-(R/S)-sulfinyll-l-r(S)mandeloyloxymethyll-lH-benzimidazole

The diastereomers of the title compound in Example 8 were separated using reversed phase chromatography (HPLC) in the same way as in Example 7, but using the diasteromeric mixture of 6-methoxy-2-[[(4-methoxy-3,5-dimethyl-2pyridinyl)methyl]-(R/S)-sulfinyl]-l-[(S)-mandeloloxymethyl]-IH-benzimidazole instead of the (R)-mandelic ester used in Example 7. Using 2.1 g of the diastereomeric mixture, the more hydrophilic isomer, 760 mg, was obtained in a

2139653 PCT/SE94/00509

WO 94/27988 pure state as a colourless syrup.

NMR data are given below.

Example 10. Preparation of (-)-5-methoxy-2-lT(4-methoxY-3,5-dimethYl-2PVridinvl)methvl1-sulfinvl1-lH-benzimidazole

0.23 g (0.45 mmol) of the more hydrophilic diastereomer of 6-methoxy-2-[[(4methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfmyl]-l-[(R)-mandeloyloxymethyl]-lH10 benzimidazole was dissolved in 15 ml methanol. A solution of 36 mg (0.9 mmol) sodium hydroxid in 0.45 ml water was added, and after 10 minutes the mixture was evaporated on a rotavapor. The residue was partitioned between 15 ml water and 15 ml dichloromethane. The organic solution was extracted with 15 ml water and to the combined aqueous solutions was added 85 pi (1.4 mmol) methyl formate. After 15 minutes the mixture was extracted with 3x10 ml dichloromethane. The organic solution was dried over Na2SO^ and then evaporated. There was obtained 0.12 g (77%) of the title compound as a colourless syrup. The optical purity (e.e.) which was analyzed by chiral column chromatography was 94%. [α]^θ= -155° (c=0.5%, chloroform).

NMR data are given below

Example 11. Preparation of (+)-5-methoxv-2-ff(4-methoxy-3,5-dimethyl-2pyridinvDmethvn-sulfinyll-lH-benzimidazole

0.76 g (1.5 mmol) of the more hydrophilic diastereomer of 6-methoxy-2-[[(4methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-l-[(S)-mandeloyloxyrnethyl]-lHbenzimidazole was dissolved in 50 ml methanol. A solution of 0.12 mg (3.0 mmol) sodium hydroxid in 1.5 ml water was added, and after 10 minutes the mixture was evaporated on a rotavapor. The residue was partitioned between 25 ml water and

WO 94/27988

PCT/SE94/00509 ml dichloromethane. The organic solution was extracted with 25 ml wateT and to the combined aqueous solutions was added 200 μΐ (3.2 mmol) methyl formate. After 15 minutes the mixture was extracted with 3x25 ml dichloromethane. The organic solution was dried over Na2SO^ and then evaporated. There was obtained

0.42 g (81%) of the title compound as a colourless syrup. The optical purity (e.e.) which was analyzed by chiral column chromatography was 98%. [α]θθ= +157° (c=0.5%, chloroform).

NMR data are given below

Table 2.

Ex. Solvent 6. CDC1₃

500 MHz

7. CDC1₃500 MHz

8. CDC1₃500 MHz

NMR data δ ppm

2.18 (s, 3H), 2.20 (s, 3H), 2.36 (s, 3H), 2.39 (s, 3H),

3.77 (s, 3H), 3.78 (s, 3H), 3.82 (s, 3H), 3.87 (s, 3H), 4.80 (d, 1H), 4.88 (d, 1H), 5.0 (m, 2H), 5.34 (s, 2H), 6.43 (d, 1H), 6.54 (d, 1H), 6.6-6.7 (m, 2H), 6.90 (d, 1H), 6.95-6.98 (m, 2H), 7.01 (d, 1H), 7.2-7.3 (m, 6H), 7.37 (m, 2H), 7.44 (m, 2H), 7.58 (d, 1H), 7.62 (d, 1H), 7.95 (s, 1H), 7.97 (s, 1H).

2.20 (s, 3H), 2.36 (s, 3H), 3.78 (s, 3H), 3.82 (s, 3H),

4.80 (d, 1H), 5.00 (d, 1H), 5.35 (d, 1H), 6.43 (d, 1H), 6.63 (d, 1H), 6.90 (d, 1H), 6.97 (dd, 1H), 7.2-7.3 (m, 3H), 7.37 (m, 2H), 7.62 (d, 1H), 7.97 (s, 1H).

2.19 (s, 3H), 2.20 (s, 3H), 2.36 (s, 3H), 2.39 (s, 3H), 3.77 (s, 3H), 3.78 (s, 3H), 3.83 (s, 3H), 3.87 (s, 3H), 4.80 (d, 1H),

4.88 (d, 1H), 5.0 (m, 2H), 5.34 (s, 2H), 6.43 (d, 1H), 6.54 (d, 1H), 6.6-6.7 (m, 2H), 6.90 (d, 1H), 6.96-6.98 (m, 2H), 7.01 (d, 1H), 7.2-7.3 (m, 6H), 7.37 (m, 2H), 7.44 (m, 2H), 7.58 (d

CA 02139653 2000-11-07

23940-831(S)

CDC1₃500 MHz

10. CDC1₃

300 MHz

11.

CDC1

1H), 7.62 (d, 1H) , 7.95 (s, 1H) , 7.97 (s, 1H) .

2.20(s,3H) ,2.36(s,3H),3.78 (s,3H) ,3.82 (s, 3H),4.80(d,1H),5.00(d,1H),5.35(d,1H),6.43 (d,lH),6.63(d,lH)6.90(d, 1H) ,6.97(dd,lH) , 7.2-7.3(m,3H),7.37(m,2H),7.62(d,1H),7.97 (S,1H).

2.18, (s, 3H) ,2.22 (s,3H) , 3.68 (S,3H) ,3.83 {s, 3H),4.77(m,2H),6.93(dd,1H),«7.0(b,1H), »7.5(b,lH),8.19(s,lH).

2.21 (s,3H) ,2.23(s,3H) ,3.69(s,3H) ,3.84 (s,3 H),4.76(m,2H),6.94(dd,1H), »7.0(b,1H),«7.5 (b,1H),8.20(s,1H).

The best mode of carrying out the invention known at present is to use the sodium salts of the optically pure compounds of the invention, thus the compounds described in Example 1 and Example 2.

Pharmaceutical preparations containing the compounds of the invention as active ingredient are illustrated in the following formulations.

Syrup

A syrup containing 1% (weight per volume) of active 10 substance was prepared from the following ingredients:

Compound according to the invention 1.0 g

Sugar, powder 30.0 g

Saccharine 0.6 g

Glycerol 5.0 g

Flavouring agent

0.05 g

CA 02139653 2000-11-07

23940-831(S)

Ethanol 96% 5.0 g

Distilled water q.s. to a final volume of 100 ml

Sugar and saccharine were dissolved in 60 g of warm water. After cooling the active compound was added to the sugar solution and glycerol and a solution of flavouring agents dissolved in ethanol were added. The mixture was diluted with water to a final volume of 100 ml.

Enteric-coated tablets

An enteric coated tablet containing 50 mg of active compound was prepared from the following ingredients:

I Compound according to the invention 500 g as Mg salt

Lactose 700 g

Methyl cellulose 6 g

Polyvinylpyrrolidone cross-linked 50 g

Magnesium stearate 15 g

Sodium carbonate 6 g

Distilled water q.s.

II Cellulose acetate phthalate 200 g

Cetyl alcohol 15 g

Isopropanol 2000 g

Methylene chloride 2000 g

I Compound according to the invention, powder, was mixed with lactose and granulated with a water solution of methyl cellulose and sodium carbonate. The wet mass was forced

CA 02139653 2000-11-07

23940-831(S) through a sieve and the granulate dried in an oven. After drying the granulate was mixed with polyvinylpyrrolidone and magnesium stearate. The dry mixture was pressed into tablet cores (10 000 tablets), each tablet containing 50 mg of active substance, in a tabletting machine using 7 mm diameter punches.

II A solution of cellulose acetate phthalate and cetyl alcohol in isopropanol/methyiene chloride was sprayed onto the tablets I in an Accela Cota^R , Manesty coating equipment. A final tablet weight of 110 mg was obtained.

Solution for intravenous administration

A parenteral formulation for intravenous use, containing 4 mg of active compound per ml, was prepared from the following ingredients:

Compound according to the invention 4 g

Sterile water to a final volume of 1000 ml

The active compound was dissolved in water to a final volume of 1000 ml. The solution was filtered through a 0.22 pm filter and immediately dispensed into 10 ml sterile ampoules. The ampoules were sealed.

Capsules

Capsules containing 30 mg of active compound were prepared from the following ingredients:

Compound according to the invention 300 g

Lactose 700 g

Microcrystalline cellulose 40 g

Hydroxypropyl cellulose low-substituted g

CA 02139653 2000-11-07

23940-831(S)

Disodium hydrogen phosphate 2 g

Purified water q.s

The active compound was mixed with the dry ingredients and granulated with a solution of disodium hydrogen phosphate. The wet mass was forced through an extruder and spheronized and dried in a fluidized bed dryer.

500 g of the pellets above were first coated with a solution of hydroxypropyl methylcellulose, 30 g, in water, 750 g, using a fluidized bed coater. Afer drying, the pellets were coated with a second coating as given below:

Coating solution:

Hydroxypropyl methylcellulose phthalate 70 g

Cetyl alcohol 4 g

Acetone 200 g

Ethanol 600 g

The final coated pellets were filled into capsules.

Suppositories

Suppositories were prepared from the following ingredients using a welding procedure. Each suppository contained 40 mg of active compound.

Compound according to the invention 4 g

Witepsol *H-15 180 g *Trade-mark

CA 02139653 2000-11-07

23940-831(S)

The active compound was homogeneously mixed with Witepsol* H-15 at a temperature of 41°C. The molten mass was volume filled into pre-fabricated suppository packages to a net weight of 1.84 g. After cooling the packages were heat sealed.

Each suppository contained 40 mg of active compound.

Stability towards racemization at different pHs

The stability of the optically pure compounds of the invention towards racemization has been measured at low concentrations in refrigerator in aqueous buffer solutions at pH 8, 9.3, 10 and 11.2. The stereochemical stability was measured by comparing the optical purity for the (-)-isomer of 5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-lH-benzimidazole in buffer solution immediately after dissolving and after several days. The measurement was performed by chromatography on an analytical chiral column. The surprising high stereochemical stability in alkaline conditions for the compounds of invention is exemplified by the fact that no racemization for the test compound was obtained at pH 11.2 even after 21 days. At pH 8,

9.3 and 10, the chemical degradation of the compound is more apparent which makes the racemization measurement more difficult to perform, however at none of these pH values a detectable racemization was obtained after 16 days.

In another racemization experiment with the optically pure compounds of the invention, an aqueous phosphate buffer solution (pH=ll) of the (+)-isomer of 5-methoxy-2-[[(4-methoxy3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-ΙΗ-benzimidazole (c = 10'⁵M) was warmed for 26 hours at 37°C without any racemization at all being observed.

*Trade-mark