Adsorbent for the removal of blood cells

Adsorption object for specification blood cell removal Technical field

For the blood cell removal for removing the white corpuscles and blood platelets which are contained in the present invention and blood It is related with an adsorption object. Background art

Recent-years and white corpuscle adsorption machines are inflammatory bowel disease (I B D) and rheumatoid arthritis. (R A) Treatment It is beginning to spread as a Treatment device. a white corpuscle adsorption machine -- principle of adsorption * filtration it uses -- removing the white corpuscles leading to inflammation directly out of blood -- medical treatment An effect is demonstrated. It is the greatest feature that there are few medical treatment and differences with a white corpuscle adsorption machine according to a medicine by medical treatment, and side effects. Filter of the method using the carrier which had a certain fixed surface coarseness in the white corpuscle adsorption machine put in practical use, or super-thin polymers textiles The used method is proposed.

patent documents 1 -- a center line average coarseness R a value -- 0 .2// pi! it is * iota omicron omicron mu m -- a concavo-convex average interval S m value -- 5 mu piiota*2 0 0; The concavo-convex surface in the range of u m The carrier for granular leukocyte adsorption which it has is indicated.

In patent documents 2, a number average molecular weight is 1. 0 0 0 It is zero or more, a coagulation value -- different -- Solvent which has compatibility for at least two sorts of polymer to each polymer it dissolves -- solidifying the polymer fluid in the solidification liquid in which a non-solvent is contained by liquid drop-like voice The manufacturing method of the porous bead which carries out Manufacture is proposed.

It is arrangement regularly highly about textiles according to organic polymer in patent documents 3. It carries out, i.e., it arranges in parallel substantially and circulates blood in the meantime, Destruction of the blood cell which was difficult to protect with filters, such as a nonwoven fabric, solidification of blood, etc. The art which catches white corpuscles to a fiber surface is indicated, conquering a problem. It is mainly Condylar from the blood of these methods, a cancer patient, or the patient that caused abnormalities to the immunity system. It was devised in order to remove white corpuscles, such as Bulbulity and a lymphocyte. however, these days especially in research, it sets to inflammatory diseases, such as an autoimmune disease, -- not only white corpuscles but blood It is becoming clear that the blood platelets in liquid are involving as an inflammatory cell._cPrior art document

Patent documents

Patent documents 1 : Provisional Publication No. 5 -- No. 168706 gazette

Patent documents 2 : Provisional Publication No. 62 -- No. 243561 gazette

Patent documents 3 : European patent No. 1931404 specification Outline of an invention

Object of the Invention

there are few problems, such as solidification in a circuit, at the time of the present invention and blood dipping -- and white corpuscle Weaning It is in offer of the adsorption object for blood cell removal which can remove blood platelets efficiently.

The means for solving an oen subject

They are a mode with the present invention, and an adsorption object for blood cell removal. it is formed by the adsorption object for blood cell removal concerned, and hydrophobic polymers resin -- surface center line average coarseness (R a) is 5*100 n m

By using the adsorption object for blood cell removal of this mode, they are a column and a time at the time of blood dipping. It is efficiently about white corpuscles and blood platelets, controlling that blood coagulation occurs in a way. It is removable.

it sets in the above-mentioned mode -- hydrophobic polymers resin is denoted by following chemical-formula (1) -- winding It may be polyarylate resin which has a return unit.

<img file="WO2009128564A1_D0001.tif" />In chemical-formula (1), the carbon number of R 1 and R 2 is a low-grade Alkyl machine of 1*5. It is, and R 1 and R 2 may be the same respectively, or they may be different. it sets in the above-mentioned mode -- hydrophobic polymers resin may also contain the polyether sulphone resin which has a manipulation return unit denoted by following chemical-formula (2) or chemical-formula (3).

<img file="WO2009128564A1_D0002.tif" />In chemical-formula (2), the carbon number of R 3 and R 4 is a low-grade Alkyl machine of 1*5. It is, and R 3 and R 4 may be the same respectively, or they may be different.

<img file="WO2009128564A1_D0003.tif" />

it sets in the above-mentioned mode -- hydrophobic polymers resin is denoted by above-mentioned chemical-formula (1) -- winding The poly arylate * fat which has a return unit, and the polyether sulphone resin which has a repeat unit denoted by above-mentioned chemical-formula (2) or chemical-formula (3) are included. It is good. It may be an adsorption object for blood cell removal of the above-mentioned mode, and bead-shaped. The above-mentioned voice The adsorption objects [ like ] for blood cell removal may be textiles of the shape of a hollow fiber, or the shape of solid thread. the white corpuscles in the adsorption object for blood cell removal of the above-mentioned mode, and blood and removal of blood platelets -- using -- Be able to.

It is Special by what combined suitably each element mentioned above, and this patent application. It may be contained in the scope of the invention which asks for protection by Pard. EFFECT OF THE INVENTION

according to the present invention, there are few problems, such as solidification in a circuit, at the time of blood dipping -- and blood The white corpuscles and blood platelets which are contained are efficiently removable. brief explanation of the drawings Schematic diagram of the porous bead manufacture device used for manufacture of Drawing 1 and the bead for blood cell removal Because -- To .

Drawing 2, Outline of the blood cell removal module using the bead for blood cell removal concerning Example 1 It is an exploded perspective view showing an important point.

Drawing 3 -- A FM image [ (1 0 / mX l O/i m) ] of the bead for blood cell removal of Example 3 -- Ah.

Drawing 4, It is A FM image of the bead for blood cell removal of comparative example 3 (1 0// mX 1 0;u m).

Drawing 5, Part which shows the outline of the blood cell removal module using the hollow fiber concerning Example 4 It is a solution perspective view.

Drawing 6 It is a perspective view of the structure of the blood cell removal module concerning A and an embodiment. Figure 6 B is an exploded perspective view of the structure of the blood cell removal module concerning an embodiment. Drawing 7 -- an example of the structure of the module for blood cell removal in an embodiment of the invention It is a perspective view.

Drawing 8 -- decomposition slant of an example of the module for blood cell removal in an embodiment of the invention It is View.

Drawing 9 -- 1 of the manufacturing method of the module for blood cell removal in an embodiment of the invention It is a figure explaining an example.

Drawing 1 it is explanation about the composition of the adsorption object for cylindrical blood cell removal in 0 and an embodiment of the invention A of 8 -- it is a figure to carry out -- a figure -- it is a figure showing an example of the section which met A line.

Drawing 1 it is explanation about the composition of the adsorption object for cylindrical blood cell removal in 1 and an embodiment of the invention A of 8 -- it is a figure to carry out -- a figure -- it is a figure showing other examples of the section which met A line. The form for inventing

[A 1st embodiment]

it is formed by the adsorption object for blood cell removal concerning an embodiment, and hydrophobic polymers resin -- the surface center line average coarseness [ (R a) ] -- 5*1 0 It is 0 nm.

By using the surface quality of the material as hydrophobic polymers resin, it is a hydrophobic interaction. It can raise adsorbent [ of white corpuscles, such as a granular leukocyte and a lymphocyte, and blood platelets ]. To.

As hydrophobic polymers resin, they are polyarylate resin (PAR) and the poly Ethers. Luhon resin (PE S), polysulfone acid resin (PE S), or these resin A polymer alloy is preferred.

Poly arylate It has a repeat unit denoted by following chemical-formula (1). It is Resin. As for the number average molecular weight of polyarylate resin, it is preferred that it is 20, 000*30, and 000. The number average molecular weights of polyarylate resin are 30 and 0. Since surface unevenness will become large too much if larger than 00, proper surface unevenness is formed. Carrying out becomes difficult. on the other hand -- the number average molecular weight of poly arylate * fat -- 20 and 0 if smaller than 00, the intensity of the adsorption object for blood cell removal will become low -- adsorption object for blood cell removal The manufacture yield worsens.

<img file="WO2009128564A1_D0004.tif" />In chemical-formula (1), the carbon number of R 1 and R 2 is a low-grade Alkyl machine of 1*5. It is OK, even if it is, R1 and R 2 are the same respectively and it is different. R As 1 and R 2, a methyl group, a Ethyl machine, a propyl group, a Buchir machine, a pentyl machine, etc. are mentioned, for example. They are desirable R 1 and R 2, and a methyl group. the repeat unit denoted by polyarylate resin and a chemical formula (1) -- main -- Unless there is no restriction especially as long as it considers it as a repeat unit, and the object of the present invention is checked others -- the repeat unit may be contained. It is front I at polyether sulphone resin and following chemical-formula (2) or chemical-formula (3). It is resin which has a repeat unit carried out. Number average of polyether sulphone resin A molecular weight is 1. It is preferred that it is 5,000*30 and 000. Polyether When the number average molecular weight of sulfone resin is larger than 30,000, surface unevenness is greatly. In order to pass, it becomes difficult to form proper surface unevenness. on the other hand -- Polyethylene the number average molecular weight of 1 Tell sulfone resin -- 1 if smaller than 5 and 000 -- object for blood cell removal the intensity of an adsorption object becomes low -- the manufacture yield of the adsorption object for blood cell removal worsens.

<img file="WO2009128564A1_D0005.tif" />In Science type (2), the carbon number of R 3 and R 4 is a low-grade Alkyl machine of 1*5. It is, and R 3 and R 4 may be the same respectively, or they may be different. R As 3 and R 4, a methyl group, a Ethyl machine, a propyl group, a Buchir machine, a pentyl machine, etc. are mentioned, for example. They are desirable R 1 and R 2, and a methyl group.

<img file="WO2009128564A1_D0006.tif" />It is R a of the surface of the adsorption object for blood cell removal 5*1 0 By being referred to as 0 n m, they are white corpuscles. And it can raise adsorbent [ of blood platelets ] more. Adsorption for blood cell removal A manufacture top is difficult for making R a of the bodily surface smaller than five. On the other hand, it is blood cell removal. If R a of the surface of the for adsorption object is larger than lO O n m, while the contribution to adsorption of blood platelets (size 2* 4 m) will decrease, manufacture by the solidifying method becomes difficult. Blood cell removal R a of the surface of the adsorption object for The past is A F M (atomic force microscope). Measuring It can do. A The measurement region by F M is 1. 0^m X 1 It is 0 mum. It is preferred to use for the removal treatment of the adsorption object for blood cell removal concerning an embodiment and white corpuscle Weaning blood platelets. being concrete and the adsorption object for blood cell removal concerning an embodiment -- power it is filled up in rum -- shedding blood in a column -- the white corpuscles from blood, and Small blood A board is removable. in this case, the inside of a column -- the adsorption object for blood cell removal -- To fill of To, a channel is formed between the adjoining adsorption objects for blood cell removal -- reservation of a blood flow Since it becomes easy, generating of blood coagulation is controlled. furthermore -- using a complicated device there is no To -- removing white corpuscle Weaning blood platelets from blood simple and efficiently Last . Fiber, such as a form of the adsorption object for blood cell removal, bead shape, the shape of a hollow fiber, or the shape of solid thread Maintenance is preferred.

case where the form of the adsorption object for blood cell removal is bead-shaped ( being with the following and the bead for blood cell removal, and obtaining) To -- it is desirable for the diameter of a bead to be 0.5*5 mm. For blood cell removal The following effects can be acquired by making an adsorption object into bead shape.

(1) L CAP (lymphocyte removal treatment) using a super-thin textiles nonwoven fabric it compares -- patient the viscosity of blood is high -- even if it is when risks, such as solidification in a column, are high -- Calah there is little Pressure drop within Beam -- problems, such as solidification, can use it few comparatively.

(2) Memory about immunity since LCAP, a difference, and a lymphocyte are not removed The danger of removing one cell decreases.

(3) GCAP (granular leukocyte removal treatment) using the adsorption material of the same bead shape It compares. Since many blood platelets are removable from The, it is inflammation condition of blood platelet origin. It can control effectively.

(4) Since medical treatment is made to the power rum of disposer pull only by passing all the blood, simple medical treatment with high safety can be provided.

(5) This which treats cheaply since expensive devices, such as a centrifuge, are not needed It can do. the form of the adsorption object for blood cell removal -- hollow fiber-like Talk solid -- case it is filar (henceforth the hollow fiber for Each blood cell removal, and solid thread for blood cell removal) To -- it is desirable for an outside diameter to be 0. 1*5 mm. the adsorption object for blood cell removal -- the shape of a hollow fiber, or solid -- being filar -- To By To, the following effects can be acquired besides the effect mentioned above.

(1) the adsorption object for blood cell removal -- the shape of a hollow fiber, or solid -- supposing that it is filar -- super-thin Fiber it compares with LCAP using Nonwoven fabric -- the viscosity of a patient's blood is high -- solidification in a column etc. -- even if it is when high-risk, it can be used comparatively satisfactorily.

(2) using the form of a hollow fiber and solid thread -- the adsorption object for blood cell removal -- raw -- the Production efficiency is improved -- a manufacturing cost can be reduced. [A manufacturing method of the bead for blood cell removal]

The manufacturing method of the bead for blood cell removal concerning an embodiment is explained. first -- sparse -- N -- methyl 2 -- water polymer resin -- pylori Boss (it calls following NM P) Dissolve. The polymer fluid (undiluted solution) It adjusts. They are solidification liquid and To about what mixed NM P in water. To. Inside diameter 0.2 It is the surface of a solidification cistern to abbreviation 1 from the nozzle of 5 mm. (refer to Drawing 1) which trickles the polymer fluid from the height of 0 c m. The bead for blood cell removal can be obtained by washing with distilled water after fully solidifying within solidification liquid.

Drawing 1, Bead manufacture device 1 for blood cell removal used for manufacture of the bead for blood cell removal 0 It is a schematic diagram of 0. Undiluted solution tank 1 1 The undiluted solution stored in 0 is pump 1. 2 It is A by 0. Jump 1 3 0 is supplied. Nozzle 1 3 The undiluted solution supplied to 0, and nozzle 1 3 It is dropped from 0. Nozzle 1 Solidification liquid bath tub 1 in which solidification liquid was stored under 30 4 0 is provided. Solidification liquid bath tub 1 4 Rotating body which makes solidification liquid start flow - spiral to 0 (not shown) It may be provided.

Solidification liquid bath tub 1 4 To the upper part of 0, it is overflow pipe' 1. 4 2 is attached. Solidification liquid bath tub 1 4 The surface of the solidification liquid stored by 0 is overflow pipe 1. 4 2 takes. When an attachment mouth is reached, overflowing solidification liquid is overflow pipe 1. 4 It is Flow about 2. Re and solidification liquid recovery tank 1 It is collected by 44. Overflow pipe 1 4 In the attachment mouth of 2, it is solidification liquid bath tub 1. Bead 1 for blood cell removal generated by 40 5 Zero paths Mesh 1 with small eyes which opens 4 It is preferred to provide 3. According to this, it is solidification liquid recovery tank 1. 4 It is bead 1 for blood cell removal to 4. 5 0 mixes as a foreign substance. It is controlled.

solidification liquid recovery tank 1 4 The solidification liquid collected by 4 and solidification liquid circulating pump 1 4 6 it is used and pumped up -- solidification liquid bath tub 1 4 It is again stored by 0. solidification liquid recovery tank 1 4 4 to solidification liquid bath tub 1 4 the quantity of the solidification liquid supplied to 0 -- flow instrument 1 4 7 -- detection -- Re and solidification liquid amount-of-supply regulating valve 1 using 48 -- a proper quantity -- solidification liquid bath tub 1 4 0 is supplied. Thus, solidification liquid is effective by circulating overflowing solidification liquid. Since use becomes possible, it is bead 1 for blood cell removal. The manufacturing cost of 50 is controlled. It can do.

nozzle 1 3 0 to solidification liquid bath tub 1 4 Inside of the undiluted solution dropped towards 0, and solidification liquid it is solidified spherically -- bead 1 for blood cell removal 5 0 is formed. It is a field in solidification liquid. B for blood cell removal good [ stable and ] the yield and more nearly spherical than liquid is dropped A 1 5 0 can be obtained.

solidified bead 1 for blood cell removal 5 0 and solidification liquid bath tub 1 4 from the lower part of 0 -- discharge -- Re and bead 1 for blood cell removal 5 Flu 1 with small eyes which opens from the path of 0 6 Are held 0. To. Solidification liquid bath tub 1 4 Bead 1 for blood cell removal discharged from 0 5 Solidification liquid containing 0 Quantity and the amount control valve 1 of solidification liquid discharge 7 It is suitably adjusted by 0. Bead 1 for blood cell removal 5 It is solidification liquid bath tub 1 in 0. 4 The solidification liquid discharged from 0 is solidification liquid recovery tank 1. 4 It is collected and reused by 4.

[A manufacturing method of the hollow fiber for blood cell removal]

The manufacturing method of the hollow fiber for blood cell removal concerning an embodiment is explained. first -- sparse -- water polymer resin is dissolved in an organic solvent -- a silk-thread-spun undiluted solution is prepared. as the organic solvent to hydrophobic polymers resin, especially if it is a right solvent, there will be no restriction -- for example, Thet Rahi Dollo Fran, dioxane, and Dimethylformamide and Dimethylacetoamide, NM P, etc. can be mentioned. the inside of these -- NM P -- as the organic solvent -- good -- better -- There.

a double nozzle is used -- a silk-thread-spun undiluted solution -- internal solidification * night (organic solvent containing water) it pushes out -- external solidification liquid (organic solvent containing water) dropping -- blood cell The hollow fiber for removal can be manufactured. Temperature at the time of spinning the hollow fiber for blood cell removal 5*1 A 5 °C grade is preferred. making silk-thread-spun temperature into this range -- silk thread spun the stability of an undiluted solution improves -- it becomes difficult to produce phase separation etc. Internal solidification liquid (core liquid) Besides As for the ratio of the concentration of part solidification liquid, it is preferred that it is 0.6*1.6.

[A manufacturing method of solid thread for blood cell removal]

The manufacturing method of solid thread for blood cell removal concerning an embodiment is explained. first -- sparse -- water polymer * fat is dissolved in an organic solvent -- a silk-thread-spun undiluted solution is prepared. as the organic solvent to hydrophobic polymers resin, especially if it is a right solvent, there will be no restriction -- for example, Thet Rahi Dollo Fran, dioxane, and Dimethylformamide and Dimethylacetoamide, NM P, etc. can be mentioned. the inside of these -- NM P -- as the organic solvent -- good -- better -- There.

the usual nozzle (orifice) it uses -- a silk-thread-spun undiluted solution -- solidification liquid (Organic dissolution agent containing water) By dropping, solid thread for blood cell removal can be manufactured. To. The temperature at the time of spinning solid thread for blood cell removal is 5*1. A 5 °C grade is preferred. Spinning by making thread temperature into this range, the stability of a silk-thread-spun undiluted solution improves -- phase separation etc. It becomes difficult to produce.

[A 2nd embodiment] .

Hereinafter, an embodiment of the invention is described with reference to drawings. Drawing 6 (A), operation It is a perspective view of the structure of the blood cell removal module concerning a form. Drawing 6 (B), operation It is an exploded perspective view of the structure of the blood cell removal module concerning a form. Blood cell removal Module - Le 1 0, case 20, and adsorption object 5 for blood cell removal It has 0.

Case 2 0 and case body 2 A [ 1 or ] pair of meshes 3 0 a and 3 0b and 1 A pair of header 4 It has 0 a and 4 O b. Case body 2 1, product made from polycarbonate It is a cylindrical member. case body 2 The material of 1, and polycarbonate it is not limited -- a well-known resin material, the charge of a metallic material, or a composite material may be used. Case body 2 A pair of meshes 3 of the product [ opening / of 1 / both-ends ] made from polyester 0 a,

3 0 b is attached, respectively. mesh 3 0 a and 3 -- it O b and mentions below Adsorption object 5 for blood cell removal it has an eye smaller than the outside diameter of 0 -- this mesh -- blood cell Adsorption object 5 for removal 0 is held in a case. Mesh 3 The material of 0 is not limited to polyester but a well-known resin material, the charge of a metallic material, or a composite material is used for it. The is also good.

case body 2 Mesh 3 mentioned above in one opening of 1 0 a -- inserting -- Buddha 4 0 a is attached. Header 4 Entrance set to 0 a with the introductory way of blood Part 2 2 is provided. Case body 2 It mentions above in the opening of another side of 1. They are a clip and header 4 about It was mesh 3 O b. 0 b is attached. Exit part 2 set to header 4 O b with the discharge way of the blood after blood cell removal 4 is provided. Header

4 0 a and header 4 It is case body 2 by 0 b. The both-ends opening of 1 is closed. To. It is header 4 in order to ensure closure. 0 a and case body 2 It reaches one, passes and is List. Da 4 0 b and case body 2 Sealing members, such as O ring, may be provided between 1.

-- A pair of mesh 3 0 a and 3 Case body 2 pinched by 0 b In the inside of 1, it is a blood cell. Adsorption object 5 for removal 0 is stored. adsorption object 5 for blood cell removal 0 and case body 2 the inside of 1 -- randomness -- in other words being arranged in irregularity and the state where it is not fixed ing. Adsorption object 50 for blood cell removal is a staple of the shape of a hollow fiber, or the shape of solid thread, and the length is case body 2. 1*6 of the inside diameter of 1 0 % is preferred and it is 1. 8*5 6 % is caused. It is desirable. Adsorption object 5 for blood cell removal It is case body 2 about the length of 0. Less than 1 % of the inside diameter of 1 The fall of carrying out and productivity is caused. On the other hand, it is adsorption object 5 for blood cell removal. The length of 0 is Kay. A main part 2 6 of the inside diameter of 1 When it becomes longer than 0 %, it is case body 2. Blood cell fibrous within one Adsorption object 5 for removal Since zero comrades interfere, Each adsorption object 5 for blood cell removal 0 is freely. Since moving is restricted, air is [ - And picking Included rare To and ] besides . case in textiles. It becomes difficult to escape. That is, the air which remained since an air omission worsened is among blood. It becomes easy to start blood coagulation. Contact surface product of the adsorption object for blood cell removal, and blood Since it decreases, the fall of adsorption performance may be caused.

Case body 2 Adsorption object 5 for blood cell removal over the capacity of 1 The filling factor of 0 is 2. 0*6 It is preferred that it is 0 %. Adsorption object 5 for blood cell removal It is a filling factor of 0 2 More than 0 %, To Since blood volume required for purification of the blood is reduced by To, a patient's burden is eased by it. It can carry out. On the other hand, it is adsorption object 5 for blood cell removal. The filling factor of 0 is 6. Size comes from 0 % and it is . To. Since it will become difficult to be filled up if it becomes, decline in working efficiency is caused. Adsorption object 5 for blood cell removal 0 consists of hydrophobic polymers resin. Thereby, it is adsorption object 5 for blood cell removal. Since the surface of 0 serves as hydrophobicity, not only a granular leukocyte but the blood platelets which are inflammatory cells are efficiently removable by a hydrophobic interaction. The inflammation condition of autoimmune disease origin can be controlled, suppressing side effects. To.

The same * fat as the embodiment of The 1 mentioned above as hydrophobic polymers resin is used. Things are made. Therefore, the explanation is omitted here.

By setting R a of the surface of the adsorption object for blood cell removal to 5*1 O O n m, they are white corpuscles. And it can raise adsorbent [ of blood platelets ] more. A manufacture top is difficult for like and the thing for which R a of the surface of the adsorption object for blood cell removal is made smaller than five which were mentioned above. -- R a of the surface of a way and the adsorption object for blood cell removal is 1. 0 If larger than 0 n m, the contribution to adsorption of blood platelets (large Tears 2*4 / m) will decrease. R a of the surface of the adsorption object for blood cell removal is A F M (atomic force microscope). It can measure. A Measurement by F M A constant field is 1. 0 i m X 1 It is 0 mu m. according to the blood cell removal module explained above, there are few stimuli to a solidification system -- blood When it uses for medical treatment as a purification column, the blood coagulation under medical treatment becomes difficult to happen. Priming (blood restoration) The air omission at the time can be made good. Since the adsorption object for blood cell removal can be manufactured in high manufacturing efficiency only by cutting the textiles of the shape of a hollow fiber in which continuous molding is possible, or the shape of solid thread, it is blood cell removal Modi. Yule's cost can be reduced.

[A 3rd embodiment]

Blood cell removal module And blood cell removal module in an embodiment of the invention A manufacturing method is explained below. it can set to the 1st and a 2nd embodiment -- said -- The same numerals are given to one composition and the explanation is omitted.

Drawing 7 and figure Like and blood cell removal module 3 in this embodiment which are shown in 8 0 0 and case 2 In zero, it is adsorption object 3 for cylindrical blood cell removal. 5 0 is stored. Kay A 2 0 and case body 2 1 and blood inflow exit 2 2, 2 it had 4, respectively -- passing -- Buddha 4 0 a and 4 accepting 0 b and necessity -- a pair of meshes 3 0 a, 3 0 It has b. On the other hand, it is adsorption object 3 for cylindrical blood cell removal. 5 0, Figure 8 And figure 9 and figure 1 Be shown in 0, the arranged shape of a plurality of hollow fiber, or solid -- adsorption object 5 for blood cell removal which consists of filar textiles Meshes-of-a-net-like cloth 5 which the both ends of 4 can blood pass Adsorption object one for blood cell removal fixed to 2 type meshes-of-a-net-like cloth 60 -- adsorption object 5 for blood cell removal involving in along the arrangement direction of 4 -- formation -- Being done. here -- adsorption object 5 for blood cell removal Meshes-of-a-net-like cloth 5 which can blood pass through the both ends of 4 both may be fixed using adhesives the case where it fixes to 2 -- again -- blood cell removal for adsorption object 5 Meshes-of-a-net-like cloth 5 which can blood pass through the both ends of 4 Even if it is made to fuse to 2 and fixes It is good. adsorption object 5 for blood cell removal the case where 4 is hollow fiber-like textiles -- the time of fixation Fusion (especially thermal melting arrival) it compares with fixation by adhesives by using -- blood cell removal The hollow of the both ends of the for adsorption object is closed easily. therefore, blood cell removal Module 3 0 a method simpler than the time of using it as 0 -- Flow of the constituent of blood into a hollow fiber ON is controlled -- residual blood is prevented. . adsorption object [ in / further / this embodiment ] 3 for cylindrical blood cell removal 5 0 and blood cell removal the manufacturing method of the for module -- a figure -- it explains using 9. in addition -- here -- adsorption object 5 for blood cell removal Meshes-of-a-net-like cloth 5 which can blood pass through the both ends of 4 The method of fixing to 2 As -- it explains taking the case of fusion. it is shown in Drawing 9 -- as -- it consists of meshes etc. Meshes-of-a-net-like cloth 5 in which blood passage is possible 2 tops -- the shape of a plurality of hollow fiber, or solid -- from filar textiles Adsorption object 5 for blood cell removal 4 is arranged -- ranking next Adsorption object 5 for blood cell removal Four both ends Fusing equipment 7 0 is used and it is meshes-of-a-net-like cloth 5. Fusion fixation is carried out 2 and it is adsorption object 5 for blood cell removal. The end of 4 is closed and they are (S 1 0 0) and adsorption object integral-type meshes-of-a-net-like cloth 6 for blood cell removal. It is To form about 0. To. here -- fusing equipment 7 Use which means of 0, thermal melting arrival, or ultrasonic fusion. It may be To. Like and adsorption object 5 for blood cell removal which are mentioned below On the other hand, 4 is meshes-of-a-net-like cloth 5 from resin. 2 consists of resin. therefore, fusing equipment 7 0 is used. things -- adsorption object 5 for blood cell removal The both ends and meshes-of-a-net-like cloth 5 of 4 Use adhesives for 2. To is fixable -- and adsorption object 5 for blood cell removal Close the end of 4. It can do. Obtained adsorption object integral-type meshes-of-a-net-like cloth 6 for blood cell removal Zero fusion portions 5 The end outside 6 may be made to remain or may be cut. Next, for [ which was obtained ] blood cell removal Adsorption object integral-type meshes-of-a-net-like cloth 6 0 should be shown in a white arrow, Adsorption object 5 for blood cell removal Meshes-of-a-net-like cloth 5 which involves in along the arrangement direction of 4 and serves as (S 1 1 0) and a spacer Adsorption object 5 for blood cell removal between which 2 was made to be placed Adsorption object 3 for cylindrical blood cell removal which consists of a bunch of 4 5 (S 1 1 2) which forms 0. Acquired adsorption object 3 for cylindrical blood cell removal 5 It is case 2 about 0. By storing in zero, the blood cell removal module in this embodiment is manufactured. adhesives are used instead of the fusion fixation mentioned above, and it fixes -- accepting necessity further -- adsorption object 5 for blood cell removal Even if it closes the both ends of 4 with adhesives It is good. acquired adsorption object 3 for cylindrical blood cell removal 5 it sets to 0 -- meshes-of-a-net-like cloth 5 2, inside of plurality empty -- filar or solid -- adsorption object 5 for blood cell removal which consists of filar textiles As the spacer of 4 it functions -- thereby case 2 Adsorption object 5 for blood cell removal stored in zero it becomes almost uniform within the distance between textiles of 4, and a blood cell removal module -- opening suitable again It will be formed. therefore, Stagnation of the blood flow in a blood cell removal module Ri is controlled -- blood cell removal module in this embodiment, blood cell adsorption efficiency it improves -- strong in an end of the adsorption object for blood cell removal still like before which consists of textiles since it is not necessary to fix using a The mesh, part mark are reducible -- a manufacturing process can be simplified.

The adsorption object for blood cell removal is 5. Absorption for blood cell removal made to fuse the case where they are four hollow fibers It wears and is body 5. Since it is closed, the hollow of the both-ends side of 4 is adsorption object 5 for blood cell removal at the time of blood cell removal. It is controlled that the blood into the hollow fiber of 4 flows and residual blood is generated. Adsorption object 3 for cylindrical blood cell removal in this embodiment 5 0 and meshes-of-a-net-like cloth 5 It is adsorption object 5 for blood cell removal of two or more layers failing in trying to be the much more on two. 4 was arranged and the both ends were fused. It can wind up the back and is formed. object for cylindrical blood cell removal in this embodiment Adsorption object 3 5 an example of the composition of zero -- A of Drawing 8 -- the figure which is a sectional view which met A line -- 1 0 and Drawing 1 It explains using 1. for example, Drawing 1 0 -- meshes-of-a-net-like cloth 5 coming out further on two -- arrangement Adsorption object 5 for blood cell removal by which fusion fixation was carried out and carried out 4 -- adsorption object 5 for blood cell removal Arrangement of 4 Adsorption object 3 for cylindrical blood cell removal along a direction by which winding-up formation was carried out 5 0 is shown. Drawing 1 1 -- meshes-of-a-net-like cloth 5 Blood cell removal by which fusion fixation was arranged and carried out by two layers on two Adsorption object 5 for The past 4 -- adsorption object 5 for blood cell removal the arrangement direction of 4 was met -- winding -- a raising -- formation -- Adsorption object 35 for Was done cylindrical blood cell removal 0 a is shown. Meshes-of-a-net-like cloth 5 They are how many layers on two. Adsorption object 5 for blood cell removal Whether 4 is made to arrange and fuse and adsorption object 5 for blood cell removal Four paths It is suitably selected by length and the viscosity of blood to dip. Next, the shape of a hollow fiber or solid thread used for the module for blood cell removal of this embodiment Adsorption object 5 for blood cell removal which consists of textiles of A state 4 is explained concretely. Blood cell removal Adsorption object 54 for The past consists of hydrophobic polymers resin. Condylar which is an inflammatory cell by a hydrophobic interaction by this since the surface of adsorption object 54 for blood cell removal serves as hydrophobicity Not only Bulbulity but blood platelets are efficiently removable. Side effects The inflammation condition of autoimmune disease origin can be controlled, stopping.

The same resin as the embodiment of The 1 mentioned above as hydrophobic polymers resin is used. Things are made. Therefore, the explanation is omitted here.

It is R a of the surface of the adsorption object for blood cell removal 5*1 By being referred to as 00 nm, they are white corpuscles. And it can raise adsorbent [ of blood platelets ] more. A manufacture top is difficult for like and the thing for which R a of the surface of the adsorption object for blood cell removal is made smaller than five which were mentioned above. On the other hand, R a of the surface of the adsorption object for blood cell removal is 1. If larger than 00 nm, the contribution to adsorption of blood platelets (large Tears 2*4 / m) will decrease. R a of the surface of the adsorption object for blood cell removal is AFM (atomic force microscope). It can measure. To this embodiment Measurement of R a of the surface of the adsorption object for blood cell removal which can be set, as AFM -- SEIKO Inn product [ made by Sturm ] "S PA400" is used -- as a probe -- "DMF S ZDF 2 OAL" (made by Seiko Instruments) it uses -- the measurement region by AFM -- 1 0 / z m X 1 0 mupiiota -- To. the adsorption object for blood cell removal which consists of textiles in this embodiment, and the shape of a hollow fiber which were mentioned above The manufacturing method of the adsorption object for blood cell removal, or manufacturing method of the adsorption object for blood cell removal of solid thread , -- it is manufactured by the solidifying method.

the outside diameter of the adsorption object for blood cell removal which consists of textiles in this embodiment is the 5-mm hollow fiber or solid thread from 0. 1 mm -- textiles in this embodiment , -- the average pole diameter of the surface of the adsorption object for blood cell removal -- 50 nm to 300 nm -- is there.

the adsorption object for blood cell removal which consists of textiles in this embodiment -- straight thread -- shape It is that. thing using a super-thin textiles nonwoven fabric (for example, Asahi Chemical Kuraray medical company make "Celsopa") it compares -- the viscosity of a patient's blood -- high -- Kay of the module for blood cell removal It can be used even if it is when risks, such as solidification, are high within A.

The meshes-of-a-net-like cloth in this embodiment, for example, a fiber diameter, (Wire diameter) 20/zm to 1 0 It is 0 mum, the number of the textiles per 1 An evening -- three to 8 the mesh of 0 (3* 8 0 mesh) is preferred -- the quality of the material which is the mesh, polyester, and Nay It is chosen out of Ron, polyethylene, and polypropylene.

The module for blood cell removal in this embodiment is L CAP (the lymphocyte removal Treatment method). Memory cell which participates in immunity since it differs and a lymphocyte is not removed The danger of removing is low. Modi for blood cell removal in this embodiment Adsorption object for cylindrical blood cell removal used for Yule, as [ mention / below ] -- GCAP (granular leukocyte removal treatment) "A Dhaka Lamb" (made by J I MRO) of the adsorption object consisting of the cell Looseji acetate bead used comparing -- it comes out to remove many blood platelets It cuts. As a result, a patient's inflammation condition can be controlled efficiently. moreover -- it is [ only passing the module for blood cell removal in this embodiment, and all the blood, and ] medical treatment since it can do, it is simple and safety is high -- an expensive device like the centrifuge used for the conventional centrifuge method is not needed. EXAMPLE

Example 1 :

Polyarylate resin (following PAR, number average molecular weight 2 5, 0 0 0) To NMP It dissolved and adjusted polymer fluid. The mass mixture ratio of PAR and NMP is 1. 5.0 : 8 It was set as 5.0. It is NMP to water 6 What carried out 0% mixture was used as solidification liquid. being concerned -- polymer fluid -- inside diameter 0.2 From the height of abbreviation 1 O c m, it was dropped [ nozzle / of 5 mm ] from the surface of the solidification cistern. it washes with distilled water after fully solidifying within solidification liquid -- the bead for blood cell removal of diameter abbreviation 1. 5 mm was obtained. bead for blood cell removal of Example 1 it is a perfect circle mostly -- rate which is shape usable as adsorption material (yield) 9 It was 9.6 % (1 0 0 zero grain calculation).

Like and bead 1 for the obtained blood cell removal which are shown in Drawing 2 9 It is cylindrical Po about 0. Casing 2 made from liquor Bonate 1 After loading into zero, one pair made from polyester Mesh 2 2 0 -- bead 1 for blood cell removal 9 State kept 0 from leaking outside Header 2 with the port of the introductory exit of blood 3 0 is attached -- module It turned. mesh 2 2 as 0 -- bead 1 for blood cell removal 9 the path of 0 -- smallness -- What has Eyes was used.

AFM measurement (Probe: l O AimX l O^un Seiko Instruments make S rho alpha4 00 and DFM S ZDF 20AL (product made from the Seiko Instruments Menthne ground)) R a of the surface of the bead for blood cell removal of Example 1 is 1. It is Sure that it is 5 nm. The private seal was carried out. Example 2 :

Polyether sulphone resin (following PE S, grade 4800 P, amount of number average molecules 2 1, 000), and N -- Methyl 2 -- Pylori Boss (henceforth, NMP) It dissolves and is Po. Limmer solution was adjusted. The mass mixture ratio of PE S and NMP is 1. 5.0 : It was set as 85.0. It is NMP to water 3 What carried out 6% mixture was used as solidification liquid. The polymer Melting concerned It is inside diameter 0.2 about liquid. It is about 1 from the surface of a solidification cistern from the nozzle of 5 mm. It was dropped from the height of 0 cm. It washed with distilled water after fully solidifying within solidification liquid, and obtained the bead for blood cell removal of diameter abbreviation 1. 5 mm. the bead for blood cell removal of Example 2 is a perfect circle mostly -- the rate which is shape usable as adsorption material -- 9 9. 8% (1 000 grains calculation) It was. AFM measurement (iota omicron mupiiotachi iota omicron mupi^ Probe: Seiko Instruments S rhoalpha400 and DFM S ZDF 20AL (product made from Seiko Instruments )) By R a of the surface of the bead for blood cell removal of Example 2 is 2. This which is 1 nm It was checked. Example 3 :

PE S (grade 4800 P, number average molecular weight 2 1, 000) and PAR (more than -- the amount 25 of common division-into-equal-parts children, and 000) -- N -- methyl 2 -- pylori Boss (henceforth, NMP) Melting It understood and adjusted polymer fluid. The mass mixture ratio of PE S, and PAR and NMP is 1. 0. 0 : 5. 0 : 8 5. It was set as 0. It is NMP to water 3 It is solidification liquid about what carried out 6% mixture. It carried out. It is inside diameter 0 about the polymer fluid concerned. 25 the nozzle of mm -- surface of a solidification cistern , -- abbreviation 1 It was dropped from the height of 0 cm. distillation after fully solidifying within solidification liquid it washes with water -- diameter abbreviation 1. 5 The bead for blood cell removal of mm was obtained. Blood cell removal of Example 3 the bead for The past is a perfect circle mostly -- the rate which is shape usable as adsorption material -- 9 9. 1 % (1 000 grains calculation) It was. A F M measurement (Probe: S PA400 by iota omicron pichichi iota omicron m and Sayko - Insturm, and DFM S ZDF 20AL (made by Seiko Instruments)) The surface of the bead for blood cell removal of Example 3 It was checked that R a is 34 nm. AFM image of Bi for blood cell removal of Example 3 - A (1 0 imX 1 0 zm) is shown in Drawing 3. Comparative example 1 :

Polymer fluid was adjusted like Example 3. It is also 70% Mixed about NMP to water. That was used as solidification liquid. It is solidification liquid from the nozzle of inside diameter 0. 25 mm about the polymer fluid concerned. The surface of a tub to abbreviation 1 It was dropped from the height of 0 c m. the inside of solidification liquid -- enough -- solidification -- To go it washes with . distilled water after It was -- the bead for blood cell removal of diameter abbreviation 1. 5 mm was obtained. the bead for blood cell removal of comparative example 1 is a perfect circle mostly -- it is shape usable as adsorption material The rate was 72. 3 % (1 000 grains calculation). AFM measurement (Probe: iotaomicron mupiiotachi iota omicron mu m and Seiko Instruments S PA400 and DFM S ZDF 2 0AL (made by Seiko Instruments)) R a of the surface of Bi for blood cell removal of comparative example 1 - A is 1. It was checked that it is 04 nm. Comparative example 2 :

As a bead for blood cell removal of comparative example 2, it is an alumina ball of diameter 2 mm. (product made from Surprise) It used. AFM measurement (Probe: 1 0 AimX 1 0 / m, S P A400 by SEIKO Instrument, and DFM S ZDF 20AL (made by the Seiko Instruments Men)) R a of the surface of the bead for blood cell removal of comparative example 2 is 1. It was checked that it is 24 nm. Comparative example 3 :

He is cell low Suasete 1 Tobey of diameter 2 mm as a bead for blood cell removal of comparative example 3. A (what was taken out from Adder column by J I MRO) was used. AFM measurement (Probe: 1 0 mu mX 1 0 mu m and Seiko Instruments S P A400 and D F M S ZDF 20AL (made by Seiko Instruments)), R a of the surface of the bead for blood cell removal of comparative example 3 is 1. It was checked that it is 34 nm. To Drawing 4, it is a A FM image of the bead for blood cell removal of comparative example 3. (iota omicron zetapiiotachi iota omicron mupiiota) It is shown. Drawing 4 shows -- as -- big unevenness in the surface of the bead for blood cell removal of comparative example 3 It exists.

[White corpuscle and a blood platelet adsorption test]

Bead for blood cell removal of the above-mentioned Example 1*3 and comparative example 1*3 (38 m L) It Column of But 27 mm in diameter, and length 7 Omm (net weight 4 OmL) It was filled up. a healthy person -- the blood of 25 OmL -- the blood back -- collecting blood -- the time of circulating by the Palin-izing back and 7 mL/m i n for 30 minutes -- granular leukocyte (neutrophil) A number, a platelet count, and the lymphocyte count are strange. From-izing, the adsorption rate to each bead for blood cell removal was computed. this result -- a table -- it is shown in 1. It Le[ Module - ]-ized like [ comparative examples / 1 thru/or 3 / Examples 2 and 3 and ] Example 1.

Table 1 :

<img file="WO2009128564A1_D0007.tif" />bead for blood cell removal of comparative example 1 (RA= 1 04 nm) -- as adsorption material -- use causing the increase in a manufacturing cost, in order that yield which becomes the made shape may decrease sharply -- stripes obtaining -- . With bead for blood cell removal of comparative example 2 (RA= 1 24 nm), they are white corpuscles and blood. Although adsorption of a small board was checked, the problem that blood coagulates during column circulation occurs. It carried out. With bead for blood cell removal of comparative example 3 (RA= 1 33 nm), it is adsorption of blood platelets. It was checked that quantity is low. With the bead for blood cell removal of Example 1*3, it is the solidification of blood the inside of column circulation, and after circulation. It is efficiently about white corpuscles and blood platelets, without generating or residual blood arising. It was checked that it can adsorb. Example 4 :

Polymer fluid was adjusted using PAR, PE S, and NMP. The weight mixture ratio of PAR and PE S is 1. : It was referred to as 1. NMP solution was used as solidification liquid and core liquid. vomiting and coming out of polymer fluid into core liquid and the above-mentioned solidification liquid using a double Spinning cap it carries out and produces the hollow fiber for blood cell removal -- this hollow fiber for blood cell removal Bundle 10,000. The hollow fiber bunch was acquired. a figure -- as [ show / in 5 ] -- this hollow fiber bunch 200 -- circle Cylindrical casing 2 made from polycarbonate 1 Polyester after loading into zero The state where the hollow fiber bunch was pressed down in a pair of meshes 220 of make, Po of the introductory exit of blood Header 230 with 1 A was attached and modularized. What has an eye smaller than the path of a hollow fiber was used as mesh 220. AFM measurement (Probe: 1 0 mu mX 1 0 mum and S P A400 made from the Seiko Instruments Menthne ground, and DF M S ZDF 20AL (made by Seiko Instruments)), It was checked that R a of the surface of the hollow fiber for blood cell removal of Example 4 is 5. 2 nm. Comparative example 4 :

Filler of Adder column which is granular leukocyte adsorption power Lamb as comparative example 4 (cellulose of diameter abbreviation 2 m m doria Sett bead) It used. AFM measurement (Probe: 1 0 z m X I 0 m, Seiko Instruments S P A 400, and D FM S ZD F 20AL (made by Seiko Instruments)) Bead of comparative example 4 Surface R a is 1. 3 It was checked that it is 3 nm. [White corpuscle and blood platelet adsorption test] .

Column of diameter 27 mm length 7 Omm (net weight 40 mL) 3260 cm²The bead of hollow fiber Talk comparative example 4 for blood cell removal of considerable Example 4 is filled up with each. It carried out. a healthy person -- the blood of 25 OmL -- the blood back -- collecting blood -- Paris the time of circulating by 7 mLZm i n after A-izing for 30 minutes -- granular leukocyte (neutrophil) A number, a platelet count, and lymph The adsorption rate to each module was computed from change of the pitch count. this result -- a table -- it is shown in 2. Table 2 :

<img file="WO2009128564A1_D0008.tif" />It is a granular leukocyte when [ which are shown in Table 2 ] the hollow fiber for blood cell removal of Example 4 is used, like and. And thing for which both blood platelets are removed efficiently It was checked. On the other hand, when the bead of comparative example 4 was used, removal of blood platelets was insufficient. Example 5 :

Polyarylate resin (following PAR; number average molecular weight 25, 000, product made from Junichika) brand name : U polymer and polyether sulphone resin (following PE S, grade 4 800 P, number-average-molecular-weight 2 1, 000 and the Sumitomo Chemical make, and brand name : Sumikaeta cell PE S) When N -- Methyl pylori boss (NMP) is used, and it is a polymer undiluted solution. It adjusted. P The weight mixture ratio of AR, P E S, and NMP is 7. 5 : 7. 5 : 85. It was referred to as 0. N -- Methyl pylori Boss solution (what mixed NMP 60% in water) It was considered as solidification liquid and core liquid. using a double pipe silk-thread-spun cap for the polymer undiluted solution mentioned above breathing out into solidification liquid with Core liquid -- it is production about a hollow fiber -- it cuts continuously -- 0.3 mm of outside diameters, and inside diameter 0. Adsorption object for blood cell removal of the hollow fiber-like staple of 2 mm and length 5 mm It obtained. A means to cut a hollow fiber to a staple, It is industry although not limited in particular. Well-known fiber cutting device using a standpoint [-like ] to a cutter roll (real extraction of a square root [ -- 2 ] 5 -- 9 60 3 No. 3 gazette, Provisional-Publication-No. 912 7 7 1 No. 90 gazette, real extraction of a square root 6 When [ gazette No. 7092 ] reference) It is suitable.

The place which measured the surface coarseness of the adsorption object for blood cell removal of this example using AFM (Probe: iota omicron piiotachi iota theta pi* and Seiko Instruments S rho alpha400 and DFM S ZDF 20A L (made by Seiko Instruments)), R It was a = 6. 2 nm. They were an average pole diameter and 25. 4 nm. an average -- thin -- an aperture and Porosi meter [ (PoreMaster- 60) ] made from Ursa Ionitas, Inc. -- business Were and it measured.

as white corpuscle and a blood platelet adsorption test -- adsorption material -- Po 27 mm in inside diameter, and 70 mm in length Case made from liquor Bonate (column) it is filled up -- outside surface area conversion -- abbreviation 0. l m²It was filled up with Of short fibers. The ratios of the length of the adsorption object for blood cell removal over the inside diameter of a case are 5 mm / 2. 7 mmX 1 00 = 1 It is 8. 5%. Blood cell removal to the capacity of a case The filling factor of the adsorption object for The past is 48 %.

a healthy person -- the blood of 25 OmL -- blood pack blood collecting -- carrying out -- the time of circulating by the Palin-izing back and 7mL/ m i n for 30 minutes -- granular leukocyte (neutrophil) A number, a platelet count, and the lymphocyte count From change, the adsorption rate to the adsorption object for blood cell removal concerning Example 1 was computed. As a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count, Each, 54%, 2%, and 6 It was 1 %.

the granular leukocyte as the adsorption object for blood cell removal concerning Example 5, and an inflammatory cell, and blood platelets -- an opposite -- sufficient adsorption ability to carry out is shown -- lymphocyte to leave the inside of the body as a memory cell Adsorption ability was low. That air omission at the time of priming is also easy, and solidification of blood It did not see. Example 6 :

a hollow fiber is produced by the same technique as Example 5 -- the obtained hollow fiber -- length 1 0 mm (a 0.3 mm outside diameter and inside diameter 0. 2 mm) it cuts -- Blood cell removal of a hollow fiber-like staple The adsorption object for The past was acquired. The ratio of the length of the adsorption object for blood cell removal over the inside diameter of a case is 1. 0 mm / 27 mmX 1 00 = 3 It is 7. 0%. Blood cell to the capacity of a case They are a filling factor of the adsorption object for removal, and 36%.

It is a blood cell adsorption test like [ object / for blood cell removal / concerning Example 6 / adsorption ] Example 1. It carried out. as a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count -- But -- Re and 5 They were 5%, 3%, and 58%.

the granular leukocyte as the adsorption object for blood cell removal concerning Example 6, and an inflammatory cell, and blood platelets -- an opposite -- sufficient adsorption ability to carry out is shown -- lymphocyte to leave the inside of the body as a memory cell Adsorption ability was low. That air omission at the time of priming is also easy, and solidification of blood It did not see. Example 7 :

a hollow fiber is produced by the same technique as Example 5 -- the obtained hollow fiber -- length 1 5 mm (a 0.3 mm outside diameter and inside diameter 0. 2 mm) it cuts -- Blood cell removal of a hollow fiber-like staple The adsorption object for The past was acquired. The ratio of the length of the adsorption object for blood cell removal over the inside diameter of a case is 1. 5 mm / 27mmX 1 It is 00 = 55. 6 %. Blood cell to the capacity of a case They are a filling factor of the adsorption object for removal, and 21%.

It is a blood cell adsorption test like [ object / for blood cell removal / concerning Example 7 / adsorption ] Example 1. It carried out. as a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count -- But -- Re and 5 They were 1 %, 3%, and 55%.

the granular leukocyte as the adsorption object for blood cell removal concerning Example 7, and an inflammatory cell, and blood platelets -- an opposite -- sufficient adsorption ability to carry out is shown -- lymphocyte to leave the inside of the body as a memory cell Adsorption ability was low. That air omission at the time of priming is also easy, and solidification of blood It did not see. Comparative example 5 :

a hollow fiber is produced by the same technique as Example 5 -- the obtained hollow fiber is cut to length 20 mm (a 0.3 mm outside diameter and inside diameter 0. 2 mm) -- Blood cell removal of a hollow fiber-like staple The adsorption object for The past was acquired. The ratios of the length of the adsorption object for blood cell removal over the inside diameter of a case are 2 Omm / 2. 7mmX 1 It is 00 = 74. 1 %. Blood cell to the capacity of a case The filling factor of the adsorption object for removal is 1. It is 5%. It is a blood cell adsorption test like [ object / for blood cell removal / concerning comparative example 5 / adsorption ] Example 5. It carried out. as a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count -- But -- They were Re, 53%, 2%, and 57%.

the granular leukocyte as the adsorption object for blood cell removal concerning comparative example 5, and an inflammatory cell, and blood platelets -- an opposite -- sufficient adsorption ability to carry out is shown -- lymphocyte to leave the inside of the body as a memory cell Adsorption ability was low. However, since the motion of the textiles within a case was restricted, air bubbles were caught in textiles and blood coagulation was looked at by the being [ air omission at the time of priming / difficult ], and end back of circulation, and the adsorption object for blood cell removal. Example 8 :

The polymer undiluted solution was adjusted using PAR (brand-name : number average molecular weight 25, 000, the product made from Junichika, and U polymer), and NMP. The weight mixture ratio of PAR and NMP is 1. 5 : It was referred to as 85. N -- methyl pylori boss solution (NMP is mixed 60% in water and it is It was) It was considered as solidification liquid. using a silk-thread-spun cap for the polymer undiluted solution mentioned above -- solidification liquid breathing out to inside -- it is production about a solid fiber membrane -- it cuts continuously -- outside diameter 0.2 5 mm and 5 m in length_mThe adsorption object for blood cell removal of the The solid filar staple was acquired. Blood cell removal to the inside diameter of a case The ratio of the length of the adsorption object for The past, and 5mmZ2 7mmX 1 00 = 1 It is 8. 5%. They are a filling factor of the adsorption object for blood cell removal to the capacity of a case, and 42%.

The place which measured the surface coarseness of the adsorption object for blood cell removal of this example using AFM (Probe: l O /zmX 1 0 / im, Seiko Instruments S P A 400, and D FM S ZDF 20A L (made by Seiko Instruments)), R It was a = 3. 4 nm. An average pole diameter is 1. It was 6. 7 nm. an average -- thin -- an aperture and the Porosi meter made from Ursa Ionitas, Inc. (PoreMaster- 60) -- business Were and it measured.

It is a blood cell adsorption test like [ object / for blood cell removal / concerning Example 8 / adsorption ] Example 5. It carried out. as a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count -- But -- They were Re, 65%, 6%, and 62%.

the granular leukocyte as the adsorption object for blood cell removal concerning Example 8, and an inflammatory cell, and blood platelets -- an opposite -- sufficient adsorption ability to carry out is shown -- lymphocyte to leave the inside of the body as a memory cell Adsorption ability was low. That air omission at the time of priming is also easy, and solidification of blood It did not see. Example 9 :

a solid fiber membrane is produced by the same technique as Example 8 -- the obtained solid fiber membrane -- length 1 0 mm (0.25 mm outside diameter) it cuts -- acquiring the adsorption object for blood cell removal of a solid filar staple It was. The ratio of the length of the adsorption object for blood cell removal over the inside diameter of a case is 1. 0mm/2 7 mmX 1 00= 3 It is 7. 0%. Adsorption object for blood cell removal over the capacity of a case A filling factor is 3. It is 1 %.

It is a blood cell adsorption test like [ object / for blood cell removal / concerning Example 9 / adsorption ] Example 5. It carried out. as a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count -- But -- Re and 6 6%, 7%, and 6 It was 0%.

the granular leukocyte as the adsorption object for blood cell removal concerning Example 9, and an inflammatory cell, and blood platelets -- an opposite -- sufficient adsorption ability to carry out is shown -- lymphocyte to leave the inside of the body as a memory cell Adsorption ability was low. That air omission at the time of priming is also easy, and solidification of blood It did not see. Example 10 :

a solid fiber membrane is produced by the same technique as Example 8 -- the obtained solid fiber membrane -- length 1 5 mm (0.25 mm outside diameter) it cuts -- acquiring the adsorption object for blood cell removal of a solid filar staple It was. The ratio of the length of the adsorption object for blood cell removal over the inside diameter of a case is l SmmZS? mmX 1 00 = 5 It is 5. 6 %. Adsorption object for blood cell removal over the capacity of a case They are a filling factor and 20%.

It is a blood cell adsorption trial like [ object / for blood cell removal / concerning Example 10 / adsorption ] Example 5. Trial was carried out. As a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count and But They were But, 64%, 5%, and 58%.

example 1 The granular leukocyte as the adsorption object for blood cell removal concerning 0, and an inflammatory cell, and blood platelets receiving sufficient adsorption ability is shown -- lymph to leave the inside of the body as a memory cell The adsorption ability of the ball was low. The solidification of that air omission at the time of priming is also easy and blood was not seen. Example 1 1 :

A polymer undiluted solution is adjusted using PE S (grade 4800 P, number-average-molecular-weight 2 1, 000, the Sumitomo Chemical make, and brand name : Sumika Kexel P E S) and NMP. It was. The weight mixture ratio of PE S and NMP is 1. 5 : 8 It was referred to as 5. N -- Methyl pylori Boss solution (what mixed NMP 60% in water) It was considered as solidification liquid. poly mentioned above breathing out the Maher undiluted solution into solidification liquid using a silk-thread-spun cap -- it is production about a solid fiber membrane -- continuous -- it cuts -- a 0.25 mm outside diameter and length 1 Adsorption for blood cell removal of the solid filar staple of 0 mm The body was acquired. The ratio of the length of the adsorption object for blood cell removal over the inside diameter of a case is /2 10 mm. 7mmX 1 00 = 3 It is 7%. Adsorption object for blood cell removal over the capacity of a case A filling factor is 28%.

Surface coarseness of the adsorption object for blood cell removal of this example, When measured using AFM (Probe: l O^mX l O^m, S P A 400 made from the Seiko Instruments Menthne ground, and D FM S Z D F 20 A L (made by Sayko 1^f Sturm)), it was R a = 5. 2 nm. An average pole diameter is 1. It was 2. 4 nm. an average -- thin -- an aperture and Porosi meter [ (PoreMaster- 60) ] made from Ursa Ionitas, Inc. -- business Were and it measured.

Example 1 It is a blood cell adsorption trial like [ object / for blood cell removal / concerning 1 / adsorption ] Example 1. Trial was carried out. As a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count and But They were But, 53%, 0%, and 48%. .

example 1 The granular leukocyte as the adsorption object for blood cell removal concerning 1, and an inflammatory cell, and blood platelets receiving sufficient adsorption ability is shown -- lymph to leave the inside of the body as a memory cell The adsorption ability of the ball was low. That air omission at the time of priming is also easy, and solidification of blood It did not see. Example 1 2 :

dialyzer 1 by 2 pro companies which uses the hollow fiber made from cell low Suasete 1 A -- F B -- 1 50 F to the hollow fiber was taken out, (inside diameter 200 mu m, outside diameter 230 m) and this were cut to 1 O mm length, and the adsorption object for blood cell removal of the hollow fiber-like staple was acquired. Kay The filling factor of the adsorption object for blood cell removal to the capacity of A is 3. It is 1 %.

Example 1 It is a blood cell adsorption trial like [ object / for blood cell removal / concerning 2 / adsorption ] Example 1. Trial was carried out. As a result, the adsorption rate of the number of granular leukocytes, a platelet count, and the lymphocyte count and Each one and 5 5 %, 1 %, and 1 It was 5 %.

Example 1 Absorption to the granular leukocyte as the adsorption object for blood cell removal concerning 2, and an inflammatory cell It is Show about arrival ability. - It carried out. On the other hand, blood platelets and the adsorption ability of the lymphocyte were low. Buraimi The solidification of that air omission at the time of Tongue is also easy and blood was not seen.

the following table -- 3 -- examples 5 thru/or 1 about 2 and comparative example 5 -- adsorption object for blood cell removal The information and blood cell adsorption test which are related are shown.

Table 3 :

<img file="WO2009128564A1_D0009.tif" />Example 1 3 :

Polyarylate resin (following PAR, number average molecular weight 25, 000, product made from Junichika) brand name : U polymer and polyether sulphone resin (following PE S, grade 4 800 P, number-average-molecular-weight 2 1, 000 and the Sumitomo Chemical make, and a brand name: Sumikaeta cell PE S) When N -- Methyl pylori boss (NMP) is used, and it is a polymer undiluted solution. It adjusted. The weight mixture ratio of PAR, PE S, and NMP is 7.5. : 7.5 : 8 It was referred to as 5.0. N -- Methyl pylori Boss solution (what mixed NMP 60% in water) It was considered as solidification Liquid sauce core liquid. A discharge in solidification liquid carries out the polymer undiluted solution mentioned above with core liquid using a double pipe silk-thread-spun cap -- it is production about a hollow fiber -- it cuts continuously -- outside diameter 30 0 zm, inside diameter 200 muetaiota, and length 1 Adsorption object for blood cell removal of the hollow fiber-like staple of 2 mm It obtained.

The place which measured the surface coarseness of the adsorption object for blood cell removal of this example using AFM (1 0 mupiiotachi 1 0 muiotaeta Probe: Seiko Instruments S rho alpha400 and D FM S Z D F 20 A L (made by Seiko Instruments)), R It was a = 5. 2 nm. The average pole diameter was 25. 4 nm.

Adsorption object for blood cell removal of the shape of a hollow fiber of this example (outside diameter 3 00 xm) length 7 Omm -- abbreviation 3 -- 500 are put in order planate -- polyester * which is meshes-of-a-net-like cloth on it -- the state where mesh made from fat (Wire diameter: 70 meshes, and 7 1 / zm) was piled up, the end face of the adsorption object for Heatsi was closed 1 Ra is used -- the both ends and mesh of the adsorption object for blood cell removal are made to fuse -- blood cell removal (adsorption surface area is about 2 and 300 cm)². next -- fusing the end outside Part is pruned, respectively -- the arrangement direction of the adsorption object for blood cell removal -- Along The adsorption object for cylindrical blood cell removal which consists of a bunch of the adsorption object for blood cell removal between which the mesh used as a spacer was made to be placed by intermediary volume Crowded was acquired.

it is shown in Drawing 8 -- as -- acquired adsorption object 3 for cylindrical blood cell removal -- case 20 (full length: 70 mm, inside diameter:2 7mm) made from A 50 -- the poly car Bonneu capacity: 4 OmL -- Loading is carried out -- it is wearing about a pair header 40 a which has a blood introduction B) blood outlet head, and 4 O b it carries out -- module A for blood cell removal was produced. Comparative example 6 :

Bulking agent of Adder column (made by J IMRO) which is a granular leukocyte adsorption column (cell roast Riasete 1 Toby's of diameter abbreviation 2 mm) R a = 1 About 33 nm, it is with Example 1. Same case made from polycarbonate (full length: 7 Omm) inside diameter: 27 mm and capacity: 4 OmL is loaded -- it is wearing about a pair of headers which have a blood feed port and a blood outlet head it carries out -- module B for blood cell removal was produced.

[Valuation method]

a healthy person -- the blood of 50 OmL -- extracting -- dividing into the Palin-izing back and a blood pack every [ 250 mL ] eye each -- blood -- granular leukocyte (neutrophil) after doing 3* flowing back of by YmLZm i n change of a number, a platelet count, and the lymphocyte count -- each module for blood cell removal The adsorption rate was computed. A result is shown in the following table 4. 6 time measurement was come and carried out. It is the average value of a result.

Table 4 :

<img file="WO2009128564A1_D0010.tif" />From Table 4, it is Example 1. The direction of module A for blood cell removal of 3 is Blood cell removal of comparative example 6. Compared with module B for The past, it turns out that the adsorption rate of blood platelets is very high. Example 14 :

Adsorption object for blood cell removal of the shape of a hollow fiber acquired in example 1 3 (outside diameter 300 mupiiota) long -- 1 2 mm -- abbreviation 8 -- 000 are put in order planate -- Po which is meshes-of-a-net-like cloth on it A state which piled up mesh made of Lester resin (Wire diameter: 70 meshes and iota omicron omicron muetaiota) Voice, Heatsi 1 Ra is used -- the both ends and mesh of the adsorption object for blood cell removal were fusion carried out, and the end face of the adsorption object for blood cell removal was closed (adsorption surface area is abbreviation 0. 9 m)². next, the end outside the fused portion is pruned, respectively -- it involves in along the arrangement direction of the adsorption object for blood cell removal -- adsorption for blood cell removal between which the mesh used as a spacer was made to be placed The adsorption object for cylindrical blood cell removal which consists of a bodily bunch was acquired.

it is shown in Drawing 8 -- as -- acquired adsorption object 3 for cylindrical blood cell removal 50 -- the poly car Bonneu Case 20 (full length : Capacity: 1 8 5 mm, Inside diameter: 5 9 mm, 324mL) made from A Load. it equips with a pair header 40 a which has a blood feed port and a blood outlet head, and 4 O b -- module C for blood cell removal was produced. Reference example:

Polyarylate resin (following P AR, number average molecular weight 25, 000, product made from Junichika) brand name : U polymer and polyether sulphone resin (brand-name : following PE S, grade 4 800 P, number average molecular weight 2 1, 000 and the Sumitomo Chemical make, and Sumikaek cell PE S) When N -- Methyl pylori boss (NMP) is used, and it is a polymer undiluted solution. It adjusted. P The weight mixture ratio of AR, P E S, and NMP is 7.5. : 7.5 : It was referred to as 85.0. N -- Methyl pylori Boss solution (what carried out 6 0% mixture of the NMP at water) It was considered as solidification liquid. From the height of abbreviation 20 c m, the polymer fluid concerned was dropped [ nozzle / of inside diameter 0. 25 mm ] from the surface of the solidification cistern. it fully solidified within solidification liquid it washes with the back and distilled water -- the bead for blood cell removal of diameter abbreviation 1 mm was obtained

Abbreviation of the bead for blood cell removal obtained by the reference example 300,000 grains (adsorption table area is abbreviation 0. 9 m) (abbreviation 2 90 m L)²case made from polycarbonate (full length : Capacity: 1 8 5 mm, inside diameter:5 9mm, 3 24mL) it loads -- it has a blood feed port and a blood outlet head it equips with a pair of headers -- module D for blood cell removal was produced.

[Valuation method]

It passes and is 5 OmL/m i n about 3 L of Palin-ized bovine blood liquid (Matrick blood: 3 2%), it dips in the state of circulation in the module for blood cell removal -- object for blood cell removal 20 minutes after the appliance inlet pressure of a modular blood feed port and the exit pressure of a blood outlet head are measured -- Module The pressure loss in Le was computed. a result -- the following tables -- it is shown in 5. Table 5 :

<img file="WO2009128564A1_D0011.tif" />By Table 5, it is Example 1. Module C for blood cell removal of 4 is bead restoration of a reference example. It turns out that pressure loss is smaller than module D for blood cell removal. Example 1 Module for blood cell removal of bead restoration of module C for blood cell removal of 4 of a reference example Granular leukocyte (neutrophil) and the adsorption rate of blood platelets were equivalent to Le D.

[Additional remark]

It is as other desirable modes of the present invention, and follows. The blood cell removal module in a mode with the present invention, The blood flow before blood cell removal flows. Case where the inlet section to carry out and the exit part by which the blood flow after blood cell removal is discharged were provided, the staple-like adsorption object for blood cell removal stored in the case, and inner side of an inlet section Weaning exit part it is provided, respectively -- mesh which holds the adsorption object for blood cell removal in a case, About the staple-like adsorption object for blood cell removal stored in the case, they are a preparation and an adsorption object for blood cell removal. Length is 1*6 of the inside diameter of a case. It is 0 %.

According to this mode, it is a problem about the air omission before use, the solidification in a circuit at the time of blood circulation, etc. White corpuscles and blood platelets are efficiently removable, controlling. blood cell the length of the adsorption object for removal -- 1 of the inside diameter of a case 8*5 it is 6 % -- more -- good -- better -- Les, According to this, the above-mentioned effect can further be heightened. it sets in the above-mentioned mode -- the filling factor of the adsorption object for blood cell removal to the capacity of a case -- 2 0*6 It may be 0 %. it sets in the above-mentioned mode -- the adsorption object for blood cell removal, the shape of a hollow fiber, or solid -- filar They may be textiles. When the adsorption object for blood cell removal is made into the shape of a hollow fiber, the amount of the material used can be reduced as compared with solid thread. Blood by the side of an inner surface It has the advantage that ball adsorption is also expectable.

it sets in the above-mentioned mode -- Summer from the adsorption object for blood cell removal, and hydrophobic polymers resin Me me is also good. In this case, hydrophobic polymers resin is a table at following chemical-formula (1). It may be polyarylate resin which has a repeat unit I(ed).

<img file="WO2009128564A1_D0012.tif" />Hydrophobic polymers resin is denoted by following chemical-formula (2) or chemical-formula (3). The polyether sulphone resin which has Repeating unit may also be included.

<img file="WO2009128564A1_D0013.tif" />In chemical-formula (2), the carbon number of R 3 and R 4 is a low-grade Alkyl machine of 1*5. It is, and R3 and R 4 may be the same respectively, or they may be different.

<img file="WO2009128564A1_D0014.tif" />it sets in the above-mentioned mode -- hydrophobic polymers resin is denoted by above-mentioned chemical-formula (1) -- winding The polyarylate resin which has a return unit, and the polyether sulphone resin which has a repeat unit denoted by above-mentioned chemical-formula (2) or chemical-formula (3) are included. Ah is good. It is business to removal of the white corpuscle Talk blood platelets in the blood cell removal module of the above-mentioned mode, and blood. It may be. It is Special by what combined suitably each element mentioned above, and this patent application. It may be contained in the scope of the invention which asks for protection by Pard. As other desirable modes, it is as follows.

(I) The blood flow after the inlet section into which the blood flow before blood cell removal flows, and blood cell removal is discharged. Case where the exit part was provided, Fiber of the arranged shape of a plurality of hollow fiber, or the shape of solid thread The both ends of the adsorption object for blood cell removal which consists of Maintenance were fixed to the meshes-of-a-net-like cloth in which blood passage is possible. The arrangement direction of the above-mentioned adsorption object for blood cell removal is met in the adsorption object integral-type meshes-of-a-net-like cloth for blood cell removal. Adsorption object for cylindrical blood cell removal to involve in, It is a preparation Blood cells removal module.

(I I) it is further provided inside the above-mentioned inlet section and the above-mentioned exit part, respectively -- it is a blood cell removal module given in the above-mentioned (I) provided with the mesh which holds the above-mentioned adsorption object for blood cell removal in the above-mentioned case.

(I I I) The above-mentioned adsorption object for blood cell removal, Repeat denoted by chemical-formula (1) shown below Even if there is little polyether sulphone resin which has a repeat unit denoted by the polyarylate resin which has a Repeat unit and chemical-formula (2), or chemical-formula (3) A kind Blood cell removal A given in the above-mentioned (I) or (I I) containing hydrophobic polymers resin He is Joule.

<img file="WO2009128564A1_D0015.tif" />In chemical-formula (1), the carbon number of R 1 and R 2 is a low-grade Alkyl machine of 1*5. There, R 1, and R 2 may be the same respectively, or they may be different.<img file="WO2009128564A1_D0016.tif" />In chemical-formula (2), the carbon number of R 3 and R 4 is a low-grade Alkyl machine of 1*5. There, R3, and R 4 may be the same respectively, or they may be different.

<img file="WO2009128564A1_D0017.tif" />

(I V) It is a blood cell removal module of an one either [ of the above-mentioned (I) to the (I I I) used for the white corpuscles in blood, and removal of blood platelets ] statement.

(V) the arranged shape of a plurality of hollow fiber, or solid -- object for blood cell removal which consists of filar textiles fixing to the meshes-of-a-net-like cloth which can blood pass through the both ends of an adsorption object -- adsorption object integral-type network for blood cell removal Eye -- A state -- cloth -- process to form, It is an object for blood cell removal about the above-mentioned adsorption object integral-type meshes-of-a-net-like cloth for blood cell removal. Process of involving in along the arrangement direction of an adsorption object and forming the adsorption object for cylindrical blood cell removal, The inlet section and blood cell removal into which the blood flow before blood cell removal flows the above-mentioned adsorption object for cylindrical blood cell removal It has a process stored in the case where the exit part by which a next blood flow is discharged was provided. It is a manufacturing method of a Blood cells removal module.

(V I) In the process of forming the adsorption object integral-type meshes-of-a-net-like cloth for blood cell removal, the above-mentioned blood the adsorption object for ball removal is a hollow fiber -- the both ends of the above-mentioned adsorption object for blood cell removal -- blood passage Adsorption object for blood cell removal which carried out thermal melting arrival the case where it fixed to possible meshes-of-a-net-like cloth by thermal melting arrival Made of a blood cell removal module given in the above-mentioned (V) by which the hollow of both ends is closed It is Manufacturing method. according to the above-mentioned mode, it compares with the adsorption object for bead-like blood cell removal -- pressure at the time of blood cell removal It can lose few.

the shape of a hollow fiber of plurality [ cloth / meshes-of-a-net-like ] or solid -- object for blood cell removal which consists of filar textiles it functions as a spacer of an adsorption object -- Distance between textiles of the adsorption object for blood cell removal stored It comes out that Separation forms an opening suitable again almost uniformly within a blood cell removal module. It cuts. Therefore, stagnation of the blood flow in a blood cell removal module is controlled. blood cell which blood cell adsorption efficiency improves and consists of textiles still like before by this since it is not necessary to use by force the mesh which fixes the end of the adsorption object for removal -- parts mark are reducible -- a manufacturing process is simplified.

Adsorption for blood cell removal which carried out thermal melting arrival the case where the adsorption object for blood cell removal was a hollow fiber Since it is closed, the hollow of a bodily both-ends side is hollow of the adsorption object for blood cell removal at the time of blood cell removal. It can control that the blood into thread flows and residual blood is generated. Although the present invention was explained in detail, they are also the range of the present invention, and one given in Above. It is not limited to that. moreover 2 0 0 8 Year 4 Month 1 Application for Patent for which it Applied on the 8th 2 0 0 8-- 1 0 9 3 8 1 and 2 0 0 8 year 6 month 1 Application for patent 2 for which it applied on the 2nd 0 0 8 -- 1 5 44 1 8 and 2 0 0 8 year 1 January 2 Application for patent 2 for which it applied on the 5th 0 0 8 -- 2 9 9 5 2 1 -- and 2 0 0 9 year 4 month 1 Application for patent 2 for which it applied on the 4th 0 0 9 -- 0 9 8 2 8 Specification indicated by 9 All statements of a detailed description of the invention, a claim, and a drawing Weaning abstract are this application. It is incorporated. Industrial applicability

It is suitable for the present invention and a blood cell removal use. Explanations of letters or numerals

1 and 1 0 and 3 0 0 blood-cell removal Mojyu Nore and 2 Zero case, 2 1 Case Body and 2 2 and 2 Four Blood Inflow Exit and 3 0 a and 3 0 B Mesh, 4 0 a and 4 It is A dodge and 3 to 0 B. 5 0 and 3 5 Adsorption Object for 0 a Cylindrical Blood Cell Removal, 5 2 Meshes-of-a-Net-like Cloth and 5 Adsorption Object for 4 Blood-Cell Removal, 56 Fusion Portions, and 60 Adsorption Object Integral-type Meshes-of-a-Net-like Cloth for Blood Cell Removal, 70 fusing equipment and 1 The bead manufacture device for 00 blood-cell removal, and 1 1 0 undiluted-solution tank and 1 20 pumps and 1 30 nozzles, a 140 solidification liquid bath tub, 144 solidification liquid recovery A tank and 1 50 Bead * for blood cell removal