Aryl sulfonyls as factor xa inhibitors

TITLE ARYL SULFONYLS AS FACTOR XA INHIBITORS

FIELD OF THE INVENTION

0003This invention relates generally to aryl sulfonyls, which are inhibitors of trypsin-like serine protease enzymes, especially factor Xa, pharmaceutical compositions containing the same, and methods of using the same as anticoagulant agents for treatment and prevention of thromboembolic disorders.

0004BACKGROUND OF THE INVENTION WO 95/14683 and WO 96/38426 describe isoxazoline and isoxazole fibrinogen receptor antagonists of the formula:

0005<img file="WO0059902A2_D0001.tif" /> wherein R¹ may be a basic group, U-V may be a six-membered aromatic ring, W-X may be a variety of linear or cyclic groups, and Y is an oxy group. Thus, these compounds all contain an acid functionality (i.e., W-X-C (=0) -Y) . In contrast, the presently claimed compounds do not contain such an acid functionality.

0006WO 94/24095 describes isoxazole immunosuppressive agents of the formula:

0007<img file="WO0059902A2_D0002.tif" /> wherein D may be a variety of groups including H, phenyl, alkyl, and acyl, and E and G may be a variety of groups including aryl and heterocycle. However, the presently claimed aryl sulfonyls are not exemplified or discussed. EP 0,513,387 depicts active oxygen inhibitors which are oxazoles or thiazoles of the formula: R³ wherein X is 0 or S, R² is preferably hydrogen, and both R¹ and R³ are substituted cyclic groups, with at least one being phenyl . The presently claimed invention does not relate to these types of oxazoles or thiazoles.

0008WO 95/18111 addresses fibrinogen receptor antagonists, containing basic and acidic termini, of the formula:

0009<img file="WO0059902A2_D0003.tif" /> wherein R¹ represents the basic termini, U is an alkylene or heteroatom linker, V may be a heterocycle, and the right hand portion of the molecule represents the acidic termini.

0010The presently claimed compounds do not contain the acidic termini of WO 95/18111.

0011In U.S. Patent No. 5,463,071, Himmelsbach et al depict cell aggregation inhibitors which are 5-membered heterocycles of the formula: x₂ /^xtx₅<img file="WO0059902A2_D0004.tif" /> wherein the heterocycle may be aromatic and groups A-B-C- and F-E-D- are attached to the ring system. A-B-C- can be a wide variety of substituents including a basic group attached to an aromatic ring. The F-E-D- group, however, would appear to be an acidic functionality which differs from the present invention. Furthermore, use of these compounds as inhibitors of factor Xa is not discussed. Illig et al, in WO 97/47299, illustrate amidino and guanidino heterocycle protease inhibitors of the formula:

0012Rⁱ-Z-X-Y-W

0013wherein R¹ can be a substituted aryl group, Z is a two carbon linker containing at least one heteroatome, X is a heterocycle, Y is an optional linker and W is an amidino or guanidino containing group. Compounds of this sort are not considered part of the present invention.

0014Jackson et al, in WO 97/32583, describe cytokine inhibitors useful for inhibiting angiogenesis . These inhibitors include imidazoles of the formula:

0015<img file="WO0059902A2_D0005.tif" /> wherein Ri is a variety of e eroaryl groups, R₄ is phenyl, naphthyl, or a heteroaryl group, and R₂ can be a wide variety of groups. Jackson et al do not teach inhibition of factor Xa. Furthermore, the imidazoles of Jackson et al are not considered part of the present invention.

0016In U.S. Patent No. 5,082,949, Sohn et al depict phenyl- pyrazole-carboxylic acids of the formula:

0017<img file="WO0059902A2_D0006.tif" /> wherein Y is a halogen, X is an acid or acid derivative, and R is selected from a variety of substituents . These compounds are indicated to have plant-growth regulating properties and also to be herbicide safenerε . However, aryl sulfonyls, like those presently claimed, have not been described by Sohn et al, nor has the inhibition of factor Xa been described.

0018In U.S. Patent No. 5,658,909, DeBernardis et al depict l-aryl-3-piperazin-l' -yl propanones of the formula:

0019<img file="WO0059902A2_D0007.tif" />^r2 wherein Ari can be a 5-membered heteroaryl substituted with an optionally substituted phenyl group, Y and R₂₄ can each be H, Z can be N or CH and Ar₂ can be phenyl or one of three heteroaryls . However, no aryl sulfonyls, like those presently claimed, have been described, nor has the inhibition of factor Xa been described. Activated factor Xa, whose major practical role is the generation of thrombin by the limited proteolysis of prothrombin, holds a central position that links the intrinsic and extrinsic activation mechanisms in the final common pathway of blood coagulation. The generation of thrombin, the final serine protease in the pathway to generate a fibrin clot, from its precursor is amplified by formation of prothrombinase complex (factor Xa, factor V,

0020Ca^⁺ and phospholipid) . Since it is calculated that one molecule of factor Xa can generate 138 molecules of thrombin (Elodi, S., Varadi, K. : Optimization of condi tions for the catalytic effect of the factor IXa-factor VIII Complex: Probable role of the complex in the amplification of blood coagulation . Thromb. Res . 1979, 15, 617-629), inhibition of factor Xa may be more efficient than inactivation of thrombin in interrupting the blood coagulation system. Therefore, efficacious and specific inhibitors of factor Xa are needed as potentially valuable therapeutic agents for the treatment of thromboembolic disorders. It is thus desirable to discover new factor Xa inhibitors.

0021SUMMARY OF THE INVENTION Accordingly, one object of the present invention is to provide novel nitrogen containing aromatic heterocycles, with ortho-substituted PI groups, which are useful as factor Xa inhibitors or pharmaceutically acceptable salts or prodrugs thereof .

0022It is another object of the present invention to provide pharmaceutical compositions comprising a pharmaceutically acceptable carrier and a therapeutically effective amount of at least one of the compounds of the,- present invention or a pharmaceutically acceptable salt or prodrug form thereof . It is another object of the present invention to provide a method for treating thromboembolic disorders comprising administering to a host in need of such treatment a therapeutically effective amount of at least one of the compounds of the present invention or a pharmaceutically acceptable salt or prodrug form thereof.

0023It is another object of the present invention to provide novel compounds of formula (I) for use in therapy.

0024It is another object of the present invention to provide the use of novel compounds of formula (I) for the manufacture of a medicament for the treatment of a thromboembolic disorder.

0025These and other objects, which will become apparent during the following detailed description, have been achieved by the inventors' discovery that compounds of formula ( I ) :

0026<img file="WO0059902A2_D0008.tif" />

0027I or pharmaceutically acceptable salt or prodrug forms thereof, wherein D, E, and M are defined below, are effective factor Xa inhibitors.

0028DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS [1] Thus, in an embodiment, the present invention provides a novel compound of formula I:

0029<img file="WO0059902A2_D0009.tif" />

0030I or a stereoisomer or pharmaceutically acceptable salt thereof, wherein;

0031ring D is absent or selected from -CH₂N=CH- , -CH=NCH₂~, -

0032CH=N-CH₂CH₂-, -CH₂CH₂CH₂-, -CH=CHCH₂-, -CH₂CH=CH-, a 5-6 membered aromatic system containing from 0-2 heteroatoms selected from the group N, 0, and S; ring D, when present, is substituted with 0-2 R, provided that when ring D is unsubstituted, it contains at least one heteroatom;

0033E is selected from phenyl, pyridyl, pyrimidyl, pyrazinyl, and pyridazinyl, substituted with 0-1 R;

0034R is selected from Cl, F, Br, I, OH, Cι_₃ alkoxy, NH₂, NH(Cι_₃ alkyl), N(Cι_₃ alkyl)₂, CH₂NH₂ , CH₂NH(Cι_₃ alkyl), CH₂N(Cι_₃ alkyl)₂, CH₂CH₂NH₂, CH₂CH₂NH (Cι_₃ alkyl), and CH₂CH₂N(Cι-₃ alkyl)₂;

0035when ring D is absent, ring E is selected from phenyl, pyridyl, pyrimidyl, pyrazinyl, and pyridazinyl, and ring E is substituted with G and R' ;

0036G is selected from F, Cl, Br, I, OH, Cι_₃ alkoxy, CN,

0037C (=NR⁸ ) NR⁷R⁹ , NHC ( =NR⁸ ) NR⁷R⁹ , NR⁸CH(=NR⁷), C(0)NR⁷R⁸, (CR⁸R⁹)_tNR⁷R⁸, SH, Cι_₃ alkyl-S, S(0)R^3b, S(0)₂R^3a, S(0) NR²R^2a, and OCF₃ ;

0038R' is selected from H, F, Cl, Br, I, SR³ , CO₂R³ , NO₂, (CH₂)_tOR³, C₁-₄ alkyl, OCF₃ , CF₃ , C(0)NR⁷R⁸, and (CR⁸R⁹)_tNR⁷R⁸;

0039alternatively, G and R' combine to form methylenedioxy or ethylenedioxy;

0040M is attached to ring E or ring D, when present, and is selected from the group: <img file="WO0059902A2_D0010.tif" />

0041<img file="WO0059902A2_D0011.tif" /><img file="WO0059902A2_D0012.tif" />

0042J is 0 or S ;

0043J^a is NH or NR^la ;

0044Z is selected from a bond, C₁-.₄ alkylene, (CH₂) _rO (CH₂) _r, (CH₂)_rNR³(CH₂)_r, (CH₂)_rC(0) (CH₂)_r# (CH₂ ) _rC (0) 0 (CH₂ ) _r , ⁽CH₂)_rOC⁽0⁾ (CH₂)r/ ⁽CH₂⁾_rC ⁽0) NR (CH₂ ) _r, (CH₂)_rNR³C(0) (CH₂)_r, (CH₂ ) _r0C (0) 0 (CH₂) _r, (CH₂ ) _r0C (O) NR³ (CH₂ ) _r, (CH₂ ) _rNR³C (0) 0 (CH₂ ) _r, (CH₂)rNR³C(0)NR³⁽CH₂)r (CH₂) _rS (0)_p (CH₂ ) _r, (CH₂)rSθ2NR³(CH₂)r, (CH₂) _rNR³S0₂ (CH₂) _r, and

0045(CH₂)_rNR³S0₂NR³ (CH₂)_r, provided that Z does not form a N-N, N-O, N-S, NCH₂N, NCH₂0, or NCH S bond with ring M or group A;

0046R^la and R^lb are independently absent or selected from -(CH₂)_r-R¹'/ -CH=CH-R¹', NCH2R¹", OCH₂R¹", SCH₂R¹", NH⁽CH₂⁾2⁽CH₂⁾tR^1', 0⁽CH₂⁾2⁽CH₂)tR^1', and S ⁽CH₂) 2 (CH₂) _tR^1# ;

0047alternatively, R^la and R^lb, when attached to adjacent carbon atoms, together with the atoms to which they are attached form a 5-8 membered saturated, partially saturated or unsaturated ring substituted with 0-2 R⁴ and which contains from 0-2 heteroatoms selected from the group consisting of N, 0, and S;

0048alternatively, when Z is C(0)NH and R^la is attached to a ring carbon adjacent to Z, then R^la is a C(O) bound to Z by replacing the amide hydrogen of Z to form a cyclic imide;

0049R¹' is selected from H, C₁-₃ alkyl, F, Cl, Br, I, -CN, -CHO, (CF₂)_rCF₃, (CH₂)_rOR², NR²R^2a, C(0)R^2c, OC(0)R², (CF₂)rC0₂R^2c, S(0)_pR^2b, NR² (CH₂ ) _r0R² , CH (=NR^2c)NR²R^2a, NR²C(0)R^2b, NR²C(0)NHR^2b, NR²C(0)₂R^2a, OC (0) NR² R^2b, C(0)NR²R^2a, C(0)NR²(CH₂)_rOR², S0₂NR²R^2a, NR²S0₂R^2b, C₃_₆ carbocyclic residue substituted with 0-2 R^4a, and 5-10 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^4a; R¹" is selected from H, CH(CH₂0R²)₂, C(0)R^2c, C(0)NR²R^2a, S(0)R^2b, S(0)₂R^2b, and S0₂NR²R^2a;

0050R², at each occurrence, is selected from H, CF₃, Cι_₆ alkyl, benzyl, C₃_₆ carbocyclic residue substituted with 0-2

0051R , and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^b;

0052R^2a, at each occurrence, is selected from H, CF₃ , Cι_₆ alkyl, benzyl, phenethyl, C₃_₆ carbocyclic residue substituted with 0-2 R^b, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^b;

0053R^2b, at each occurrence, is selected from CF₃ , C₁-₄ alkoxy,

0054Cι_₆ alkyl, benzyl, C₃_₆ carbocyclic residue substituted with 0-2 R^4b, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^4b;

0055R ^c, at each occurrence, is selected from CF₃ , OH, Cι_₄ alkoxy, Cι_₆ alkyl, benzyl, C₃_₆ carbocyclic residue substituted with 0-2 R^b, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^b;

0056alternatively, R² and R² , together with the atom to which they are attached, combine to form a 5 or 6 membered saturated, partially saturated or unsaturated ring substituted with 0-2 R^b and containing from 0-1 additional heteroatoms selected from the group consisting of N, O, and S; R³, at each occurrence, is selected from H, C₁-₄ alkyl, and phenyl;

0057R³ , at each occurrence, is selected from H, C₁-₄ alkyl, and phenyl;

0058R^3b, at each occurrence, is selected from H, C₁-₄ alkyl, and phenyl;

0059R^3c, at each occurrence, is selected from C₁-₄ alkyl, and phenyl ;

0060R^3d, at each occurrence, is selected from H, OH, and Cι_₃ alkyl;

0061R^3e, at each occurrence, is selected from H and CH₃ ;

0062A is selected from:

0063C _ιo carbocyclic residue substituted with 0-2 R⁴, and 5-10 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R⁴;

0064<img file="WO0059902A2_D0013.tif" />

0065X-Y combine to form a group selected from:

0066_C(_R3d_R3e)_C(_R3d_R3e) , _{C (R}3d_R3e) _C (_R3d_R3e) _C (_R3d_R3e) _#

0067NR ^cC(R^3dR^3e) , NR^4cC(=0) , NR⁴ C(=NH) ,

0068NR^4cC(R^3dR^3e)C(R^3dR^3e) , NR^4cC (R^3dR^3e)NR ^c, NR ^cC (=0)NR ^c, NR ^cC(=0)C(R^3dR^3e) , C (R^3dR^3e)NR ^c, C (R^3dR^3e) NR^4cC (R^R³^) ,

0069C(R^3dR^3e)C(R^3dR^3e)NR ^c, NR^4cNR ^c, NR ^cNR ^cC (R^3dR^3e) , and _C(_R3d_R3e)_NR4c_NR4c. R⁴, at each occurrence, is selected from H, =0, (CH₂)_rO^R2/ ^F» Cl, Br, I, Cι_₄ alkyl, -CN, N0₂ , (CH₂) _rNR²R^2a, (CH₂)_rC(0)R^2c, NR²C(0)R^2b, C(0)NR²R^2a, NR²C (0)NR²R^2a, CH(=NR²)NR²R^2a, CH (=NS (0) R⁵) NR²R^2a, NHC (=NR²)NR²R^2a,

0070C(0)NHC(=NR²)NR²R^2a, S0NR²R² , NR²S0₂NR²R^2a, NR²S0₂-Cι_₄ alkyl, NR²S0₂R⁵, S(0)_pR⁵, (CF₂)_rCF₃, NCH₂R¹", OCH₂R¹", SCH2R^1", N⁽CH₂⁾2⁽CH₂⁾tR^1', 0⁽CH₂⁾2⁽CH₂)tR^1', and

0071S⁽CH₂⁾2⁽CH2⁾_tR¹';

0072alternatively, one R⁴ is a 5-6 membered aromatic heterocycle containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S;

0073R^4a is selected from H, =0, (CH₂)_rOR², (CH₂)_r-F/ (CH₂)_r-Br, (CH₂)_r-Cl, I, C₁-₄ alkyl, -CN, N0₂, (CH₂) _rNRR^a, (CH₂)_rNR²R^2b, (CH₂)_rC(0)R^2c, NR²C(0)R^2b, C(0)NR²R^2a, C(0)NH(CH₂)₂NR²R^2a, NR²C (0)NR²R^2a, CH (=NR²) NR²R^2a, NHC(=NR²)NR²R² , S0₂NR²R^2a, NR²S0₂NR²R^2a, NR²S0 -Cι_ alkyl, C (0)NHS0₂-Cι_₄ alkyl, NR²S0₂R⁵, S(0)_pR⁵, and (CF₂)_rCF₃;

0074R^4b, at each occurrence, is selected from H, =0, (CH₂)_rOR³, F, Cl, Br, I, C₁-₄ alkyl, -CN, N0₂, (CH₂) _rNR³R^3a, (CH₂)_rC(0)R³, (CH₂)_rC(0)0R^3c, NR³C(0)R^3a, C(0)NR³R^3a, NR³C(0)NR³R^3a, CH(=NR³)NR³R^3a, NH³C (=NR³ ) NR³R^3a, S0₂NR³R^3a, NR³S0₂NR³R^3a, NR³S0₂-Cι_ alkyl, NR³S0₂CF₃, NR³S0₂-phenyl, S(0)_PCF₃, S(0)_p-Cι_₄ alkyl, S (0)_p-phenyl, and (CF₂)_rCF₃;

0075R^4c, at each occurrence, is selected from H, C₁-₄ alkyl,

0076CH₂-CN, (CH₂)₂-CN, CH₂-NR²R^2a, (CH₂ ) ₂NR²R^2a, CH₂C(0)R^2c, (CH₂)₂C(0)R^2c, CH₂-C(0)NR²R^2a, (CH₂ ) ₂C (O) NR²R^2a, phenyl, and benzyl; R⁵, at each occurrence, is selected from CF , Cι_₆ alkyl, phenyl substituted with 0-2 R⁶, and benzyl substituted with 0-2 R⁶;

0077R⁶, at each occurrence, is selected from H, OH, (CH₂)_rOR² F» Cl, Br, I, Cι-₄ alkyl, CN, N0₂, (CH₂) _rNR²R^2a, (CH₂)_rC(0)R^2b, NR²C(0)R^2b, NR²C (0) NR²R² , CH(=NH)NH₂, NHC(=NH)NH₂, S0₂NR²R² , NR²S0₂NR²R^2a, and NR²S0₂Ci-₄ alkyl;

0078R⁷, at each occurrence, is selected from H, OH, Cχ-₆ alkyl, Cι-₆ alkylcarbonyl, Cι_₆ alkoxy, C₁-₄ alkoxycarbonyl , (CH )n-phenyl, C₆-₁₀ aryloxy, C₆-₁₀ aryloxycarbonyl, C₆-₁₀ arylmethylcarbonyl, C₁-₄ alkylcarbonyloxy C₁-.₄ alkoxycarbonyl, C₆-₁₀ arylcarbonyloxy C₁-₄ alkoxycarbonyl, -Q alkylaminocarbonyl, phenylaminocarbonyl, and phenyl Cι_₄ alkoxycarbonyl;

0079R⁸, at each occurrence, is selected from H, C -e alkyl and (CH₂)n-phenyl ;

0080alternatively, R⁷ and R⁸ combine to form a 5 or 6 membered saturated, ring which contains from 0-1 additional heteroatoms selected from the group consisting of N, 0, and S;

0081R⁹, at each occurrence, is selected from H, -e alkyl and (CH )n-phenyl;

0082n is selected from 0, 1, 2, and 3;

0083m is selected from 0, 1, and 2 ;

0084p is selected from 0, 1, and 2; r is selected from 0, 1, 2, and 3;

0085s is selected from 0, 1, and 2; and,

0086t is selected from 0 and 1.

0087[2] In a preferred embodiment, the present invention provides a novel compound of formula la or lb:

0088<img file="WO0059902A2_D0014.tif" /> la lb wherein in formula la, rings D-E represent a bicycle selected from the group: l-aminoisoquinolin-7-yl; 1,3- diaminoisoquinolin-7-yl ; 1 , 4-diaminoisoquinolin-7-yl ; 1, 5-diaminoisoquinolin-7-yl; 1, 6-diaminoisoquinolin-7- yl; l-amino-3-hydroxy-isoquinolin-7-yl; l-amino-4- hydroxy-isoquinolin-7-yl; 1-amino-5-hydroxy- isoquinolin-7-yl; l-amino-6-hydroxy-isoquinolin-7-yl; l-amino-3-methoxy-isoquinolin-7-yl; l-amino-4-methoxy- isoquinolin-7-yl; l-amino-5-methoxy-isoquinolin-7-yl; l-amino-6-methoxy-isoquinolin-7-yl; 1-hydroxy- isoquinolin-7-yl; 4-aminoquinazol-6-yl; 2,4- diaminoquinazol-6-yl; 4, 7-diaminoquinazol-6-yl 4,8- diaminoquinazol-6-yl; l-aminophthalaz-7-yl; 1,4- diaminophthalaz-7-yl; 1, 5-diaminophthalaz-7-yl; 1,6- diaminophthalaz-7-yl; 4-aminopterid-6-yl; 2,4- aminopterid-6-yl; 4, 6-diaminopterid-6-yl; 8-amino-l,7- naphthyrid-2-yl; 6, 8-diamino-l, 7-naphthyrid-2-yl; 5,8- diamino-1, 7-naphthyrid-2-yl; 4 , 8-diamino-l, 7- naphthyrid-2-yl; 3 , 8-diamino-l, 7-naphthyrid-2-yl; 5- amino-2, 6-naphthyrid-3-yl; 5, 7-diamino-2, 6-naphthyrid- 3-yl; 5, 8-diamino-2, 6-naphthyrid-3-yl; 1, 5-diamino-/2, 6- naphthyrid-3-yl; 5-amino-l, 6-naphthyrid-3-yl; 5,7- diamino-1, 6-naphthyrid-3-y; 5, 8-diamino-l, 6-naphthyrid- 3-yl; 2 , 5-diamino-l, 6-naphthyrid-3-yl; 3-aminoindazol- 5-yl; 3-hydroxyindazol-5-yl; 3-aminobenzisoxazol-5-yl; 3-hydroxybenzisoxazol-5-yl; 3-aminobenzisothiazol-5-yl; 3-hydroxybenzisothiazol-5-yl; l-amino-3 , 4- dihydroisoquinolin-7-yl; and, l-aminoisoindol-6-yl;

0089alternatively, in formula la ring D is absent and ring G is phenyl or pyridyl and ring G is substituted with G and R' :

0090G is selected from F, Cl, Br, I, OH, C₁-₃ alkoxy, CN,

0091C(=NR⁸)NR⁷R⁹, NHC (=NR⁸ ) NR⁷R⁹ , NR⁸CH(=NR⁷), C(0)NR⁷R⁸, (CR⁸R⁹)_tNR⁷R⁸, SH, Cι_₃ alkyl-S, S(0)R^3b, S(0)₂R^3a, S(0) NR²R^2a, and OCF₃ ;

0092R' is selected from H, Cl, F, Br, I, (CH₂)_tOR³, C₁-₄ alkyl, OCF₃, CF₃, C(0)NR⁷R⁸, and (CR⁸R⁹) _tNR⁷R⁸;

0093alternatively, in formula lb, rings D-E are selected from the group: 4-amino-pyrido [4, 3-b] furan-3-yl, 4-amino- pyrido[4, 3-b] furan-2-yl, 7-amino-pyrido [3 , 4-b] furan-2- yl, 4-amino-pyrido [4 , 3-b] thien-3-yl, 4-amino- pyrido [4, 3-b] thien-2-yl, 7-amino-pyrido [3 , 4-b] thien-2- yl, 4-amino-5-aza-indol-3-yl, 4-amino-5-aza-indol-2-yl, 7-amino-6-aza-indol-3-yl, 4-amino-5-aza-indol-l-yl, 7- amino-pyrido [4, 3-d] imidazol-1-yl, 4-amino-pyrido [3 , 4- d] imidazol-1-yl, 4-amino-5-aza-inden-3-yl, 4-amino-5- aza-inden-2-yl, 4-amino-5-aza-inden-l-yl, 4-amino-5- aza-dihydroinden-3-yl , 4-amino-5-aza-dihydroinden-2-yl , and 4-amino-5-aza-dihydroinden-l-yl;

0094M is selected from the group: <img file="WO0059902A2_D0015.tif" />Z is selected from (CH₂)_rC(0) (CH₂)_r, (CH₂) _rC (0) O (CH₂) _r, (CH₂)rC⁽0)NR³⁽CH₂)r, (CH₂) _rS (0) _p (CH₂) _r , and ⁽CH₂⁾rS0₂NR³⁽CH2⁾r;

0095R^3d, at each occurrence, is selected from H and CH₃ ;

0096A is selected from:

0097C₅-₆ carbocyclic residue substituted with 0-2 R⁴, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R⁴;

0098X-Y combine to form a group selected from:

0099C(R^3dR^3e)C(R^3dR^3e) , C(R^3dR^3e)C(R^3dR^3e)C(R^3dR^3e) , NR^4cC(R^3dR^3e) , NR ^cC(=0), NR ^cC (R^3dR^3e) C (R^3dR^3e) ,

0100NR^4cC(R^3dR^3e)NR^c, NR^4cC(=0)NR^4c, NR^cC (=0) C (R^3dR^3e) ,

0101C(R³ R^3e)NR ^c, C(R^3dR^3e)NR^cC(R^3dR^3e) , and _C(R3d_R3e_)C(R3d_R3e_)NR4c. _and

0102R^c, at each occurrence, is selected from H, C₁-.₄ alkyl,

0103CH₂-CN, (CH₂)₂-CN, CH₂-NRR^2a, (CH ) ₂NR²R^2a, CH₂C(0)R^2c, and (CH₂)₂C(0)R^2c.

0104[3] In a more preferred embodiment, the present invention provides a novel compound of formula la, wherein;

0105rings D-E represent a bicycle selected from the group: 1- aminoisoquinolin-7-yl, 1, 3-diaminoisoquinolin-7-yl, 1, 4-diaminoisoquinolin-7-yl, 1 , 5-diaminoisoquinolin-7- yl, 1, 6-diaminoisoquinolin-7-yl, 1-hydroxy-isoquinolin- 7-yl, 4-aminoquinazol-6-yl, 2 , 4-diaminoquinazol-6-yl, 4, 7-diaminoquinazol-6-yl, 4 , 8-diaminoquinazol-6-yl, 1- aminophthalaz-7-yl, 1, 4-diaminophthalaz-7-yl, 1,5- diaminophthalaz-7-yl, 1 , 6-diaminophthalaz-7-yl, 4- aminopterid-6-yl, 8-amino-l, 7-naphthyrid-2-yl, 5-amino- 1, 6-naphthyrid-3-y, 5-amino-2 , 6-naphthyrid-3-yl, 3- aminobenzisoxazol-5-yl, 3-aminobenzisothiazol-5-yl, 1- amino-3 , 4-dihydroisoquinolin-7-yl, and 1-aminoisoindol- 6-yl;

0106alternatively, ring D is absent and ring G is phenyl or pyridyl and ring G is substituted with G and R' :

0107G is selected from F, Cl, Br, and Cι_₃ alkoxy;

0108R' is selected from H, F, Cl, Br, OR³, and CHOR³ ;

0109M is selected from the group:

0110<img file="WO0059902A2_D0016.tif" />

0111Z is selected from (CH₂)_rC (O) (CH ) _r and (CH₂) _rC (O)NR³ (CH₂) _r;

0112A is selected from:

0113C₅-₆ carbocyclic residue substituted with 0-2 R⁴, and 5-6 membered aromatic heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R⁴;

0114X-Y combine to form a group selected from: CH₂CH₂, NR^4cCH₂ , NR^4cC(=0), and CH₂NR^4c; and, R^4c, at each occurrence, is selected from H, CH₂-CN, (CH₂)₂-CN, CH₂-NR²R^2a, and (CH₂) ₂NR²R^2a.

0115[4] In an even more preferred embodiment, the present invention provides a novel compound of formula la, wherein;

0116rings D-E represent a bicycle selected from the group: 1- aminoisoquinolin-7-yl, 1, 5-diaminoisoquinolin-7-yl, 1, 6-diaminoisoquinolin-7-yl, l-aminophthalaz-7-yl, 1,4- diaminophthalaz-7-yl, 1, 5-diaminophthalaz-7-yl, 1,6- diaminophthalaz-7-yl, 4-aminopterid-6-yl, 8-amino-l,7- naphthyrid-2-yl, 5-amino-l, 6-naphthyrid-3-y, 5-amino- 2 , 6-naphthyrid-3 -yl, 3-aminobenzisoxazol-5-yl, 4-amino- 5-azabenzothiophen-2-yl, and, l-aminoisoindol-6-yl;

0117alternatively, ring D is absent and ring E is phenyl substituted with G and R' ;

0118G is selected from CH₂NH₂ , CH₂NHCH₃ , CH(CH₃)NH₂, C(CH₂)₂NH₂, F, Cl, Br, and OCH₃ ;

0119R' is selected from H, OCH₃ , Cl, and F.

0120M is selected from the group:

0121<img file="WO0059902A2_D0017.tif" />J is N;

0122R^la and R^lb are independently absent or are - (CH₂) r^-Rl' /"

0123R¹' is selected from H, Cι_₃ alkyl, F, Cl, -CN, CF₃ ,

0124(CH₂)_rOR², NR²R^2a, C(0)R^2c, OC(0)R², S(0)_pR^2b, NR²C(0)R^2b, C(0)NR²R^2a, S0₂NR²R^2a, C₃_₆ carbocyclic residue substituted with 0-2 R^4a, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, O, and S substituted with 0-2 R^4a;

0125A is selected from phenyl, pyridyl, pyrimidyl, pyridazinyl, and pyrazinyl and is substituted with 0-2 R⁴;

0126X-Y combine to form NR^4cCH₂;

0127R², at each occurrence, is selected from H, CF₃ , C₁-₆ alkyl, benzyl, C₅-₆ carbocyclic residue substituted with 0-2 R^b, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, O, and S substituted with 0-2 R^4b;

0128R^2a, at each occurrence, is selected from H, CF₃ , C -β alkyl, benzyl, phenethyl, C₅_₆ carbocyclic residue substituted with 0-2 R^b, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^b;

0129R^2b, at each occurrence, is selected from CF₃ , Cι_₄ alkoxy,

0130C₁-₆ alkyl, benzyl, C_$-Q carbocyclic residue substituted with 0-2 R^b, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^4b; R^2c, at each occurrence, is selected from CF₃ , OH, Cι_₄ alkoxy, ±-s alkyl, benzyl, C₅-.₆ carbocyclic residue substituted with 0-2 R^b, and 5-6 membered heterocyclic system containing from 1-4 heteroatoms selected from the group consisting of N, 0, and S substituted with 0-2 R^b;

0131alternatively, R² and R^2a, together with the atom to which they are attached, combine to form a ring selected from imidazolyl, morpholino, piperazinyl, pyridyl, and pyrrolidinyl, substituted with 0-2 R^4b;

0132R⁴, at each occurrence, is selected from H, =0, OR², CH₂OR², F, Cl, C₁-₄ alkyl, NR²R^2a, CH₂NR²R² , C(0)R^2c, CH₂C(0)R^2c, C(0)NR²R^2a, S0₂NR²R^2a, NR²S0₂-Cι_ alkyl, S(0)₂R⁵, and CF₃

0133R^a, at each occurrence, is selected from H, (CH₂)_rO^R2_^ F Cl, C₁-₄ alkyl, NR²R^2a, CH₂NR²R^2a, NR²R^2b, CH₂NR²R^2b, (CH₂)_rC(0)R^2c, NR²C(0)R^2b, C(0)NR²R^2a, NR²C (0) NR²R^2a, S0₂NR²R^2a, S(0)₂R⁵/ and CF₃ ;

0134R^4b, at each occurrence, is selected from H, =0, (CH₂)_rOR³, F, Cl, C₁-₄ alkyl, NR³R^3a, CHNR³R^3a, C(0)R³, CH₂C(0)R³, C(0)OR^3c, C(0)NR³R^3a, S0₂NR³R^3a, NR³S0₂-Cι_₄ alkyl, NR³S0₂-phenyl, S(0)₂-Cι-₄ alkyl, S (O) ₂-phenyl, and CF₃ ; and,

0135R^4c, at each occurrence, is selected from H, CH₂-CN, (CH₂)₂-CN, CH₂-NH₂, and (CH₂)₂NH₂.

0136[5] In a further preferred embodiment, the present invention provides a novel compound of formula la whereii: rings D-E represent a bicycle selected from the group: 1- aminoisoquinolin-7-yl , 1 , 5-diaminoisoquinolin-7-yl , 1, 6-diaminoisoquinolin-7-yl, 8-amino-l, 7-naphthyrid-2- yl, 5-amino-l, 6-naphthyrid-3-y, 5-amino-2 , 6-naphthyrid- 3-yl, 3-aminobenzisoxazol-5-yl, l-aminophthalaz-7-yl,

01374-amino-5-azabenzothiophen-2-yl, and, l-aminoisoindol-6- yl; and,

0138alternatively, ring D is absent and R' , G, and ring E form a group selected from 3-aminomethylphenyl, 4-fluoro-3- aminomethylphenyl , 4-fluoro-3-

0139(methylaminomethyl) phenyl, 4-chloro-3-aminophenyl, and 2-aminomethylphenyl .

0140[6] In an even further preferred embodiment, the present invention provides a novel compound selected from:

0141N- [4- (1, 1-dioxido-l, 2-benzisothiazol-2-cyanomethyl-7- yDphenyl] -1- (4-methoxyphenyl) -3- (trifluoromethyl) -1H- pyrazole-5-carboxamide,-

0142N- [4- (2 , 3-dihydro-l, 1-dioxido-l, 2-benzisothiazol-7- yl) phenyl] -1- (4-methoxyphenyl) -3- (trifluoromethyl) -1H- pyrazole-5-carboxamide;

0143N- [4- (2, 3-dihydro-3-hydroxy-l, 1-dioxido-l, 2-benzisothiazol- ⁷-yl ) phenyl] -1- (4-methoxyphenyl) -3- (trifluoromethyl) - lH-pyrazole-5-carboxamide;

0144N- [4- [2- [2- (diethylamino) ethyl] -2 , 3-dihydro-l, 1-dioxido-l, 2- benzisothiazol-7-yl]phenyl] -1- (4-methoxyphenyl) -3- ( trifluoromethyl) -lH-pyrazol-5-carboxamide;

01451- [3- (aminomethyl ) phenyl ] -N- [4- (2 , 3-dihydro-l, 1-dioxido-l, 2- benzisothiazol-7-yl)phenyl] -3- (trifluoromethyl) -1H- pyrazole-5-carboxamide; 1- [3- (a inomethyl) phenyl] -N- [4- (2 , 3-dihydro-2-methyl-1, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -3- ( trifluoromethyl) -Iff-pyrazole-5-carboxamide;

0146l-[3- (aminomethyl) -4-fluorophenyl] -N- [4- (2 , 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -3- ( trifluoromethyl) -Iff-pyrazole-5-carboxamide;

01471- (3-amino-l, 2-benzisoxazol-5-yl) -N- [4- (2 , 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -3- ( trifluoromethyl) -Iff-pyrazole-5-carboxamide;

01481- [3- (aminoiminomethyl) phenyl] -N- [4- (2 , 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -3-

0149( trifluoromethyl) -lff-pyrazole-5-carboxamide;

01501- [3- (aminoiminomethyl) phenyl] -N- [4- (2, 3 -dihydro-2-methyl- 1, 1-dioxido-l, 2-benzisothiazol-7-yl) phenyl] -3- (trifluoromethyl) -lff-pyrazole-5-carboxamide;

01511- [3 - (aminoiminomethyl ) phenyl] -N- [4- [2- [2-

0152(diethylamino) ethyl] -2, 3-dihydro-l, 1-dioxido-l, 2- benzisothiazol-7-yl]phenyl] -3- (trifluoromethyl) -lff- pyrazole-5-carboxamide;

0153- [3- (aminoiminomethyl) phenyl] -N- [4- (2, 3-dihydro-l, 1- dioxido-benzo [£>] thiophen-7-yl) ) phenyl] -3- ( trifluoromethyl) -Iff-pyrazole-5-carboxamide;

0154- [3- (aminomethyl) phenyl] -N- [4- (2 , 3-dihydro-l, 1-dioxido- benzo [i>] thiophen-7-yl) ) phenyl] -3- (trifluoromethyl) -1H- pyrazole-5-carboxamide;

0155- (3- (amino-1, 2-benzisoxazol-5-yl) -N- [4- (2 , 3-dihydro-2- methyl-1, 1-dioxido-l, 2-benzisothiazol-7-yl)phenyl] -3- ( trifluoromethyl) -lff-pyrazole-5-carboxamide; 1- (3- (amino-l,2-benzisoxazol-5-yl) -N- [4- [2- [2-

0156(diethylamino) ethyl] -2, 3-dihydro-l, 1-dioxido-l, 2- benzisothiazol-7-yl) phenyl] -3- (trifluoromethyl) -lff- pyrazole-5-carboxamide;

0157l-(3- (amino-l,2-benzisoxazol-5-yl) -N- [4- (2 , 3-dihydro-l, 1- dioxido-benzo[ ] thiophen-7-yl) ) phenyl] -3- ( trifluoromethyl) -lff-pyrazole-5-carboxamide;

0158l-[3- (aminomethyl) phenyl] -N- [4- [ 2 - [2- (diethylamino) ethyl] - 2 , 3-dihydro-l, 1-dioxido-l, 2-benzisothiazol-7- yl]phenyl] -3- (trifluoromethyl) -Iff-pyrazole-5- carboxamide

01591- (3-amino-4-chlorophenyl) -N- [4- (2, 3-dihydro-l, 1-dioxido-

01601, 2-benzisothiazol-7-yl) phenyl] -3- (trifluoromethyl) -lff- pyrazole-5-carboxamide;

01611- (3-amino-4-chlorophenyl) -N- [4- (2 , 3 -dihydro-2-methyl-1, 1- dioxido-1, 2-benzisothiazol-7-yl) phenyl] -3- ( trifluoromethyl) -lff-pyrazole-5-carboxamide;

01621- (3-amino-4-chlorophenyl) -N- [4- [2- [2- (diethylamino) ethyl] - 2 , 3-dihydro-l, 1-dioxido-l, 2-benzisothiazol-7- yl] phenyl] -3- (trifluoromethyl) -lff-pyrazole-5- carboxamide;

01631- (3-amino-4-chlorophenyl) -N- [4- (2 , 3-dihydro-l, 1-dioxido- benzo [jb] thiophen-7-yl) ) phenyl] -3- (trifluoromethyl) -lff- pyrazole-5-carboxamide;

0164- [3- (aminoiminomethyl) phenyl] -N- [4- (2, 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -3- (trifluoromethyl) -lff-pyrazole-5-carboxamide; - (l-amino-7-isoquinolinyl) -N- [4- (2 , 3-dihydro-l, 1-dioxido-

01651, 2-benzisothiazol-7-yl) phenyl] -3- (trifluoromethyl) -lff- pyrazole-5-carboxamide;

0166- (l-amino-7-isoquinolinyl) -N- [4- (2 , 3-dihydro-2-methyl-1, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -3- (trifluoromethyl) -lff-pyrazole-5-carboxamide;

0167- (l-amino-7-isoquinolinyl) -N- [4- [2- [2- (diethylammo) ethyl] - 2 , 3-dihydro-l, 1-dioxido-l, 2-benzisothiazol-7- yl] phenyl] -3- (trifluoromethyl) -lff-pyrazole-5- carboxamide;

0168- (l-amino-7-isoquinolinyl) -N- [4- (2 , 3-dihydro-l, 1-dioxido- benzo [Jb] thiophen-7-yl) ) phenyl] -3- (trifluoromethyl) -lff- pyrazole-5-carboxamide;

0169- (3- (amino-1, 2-benzisoxazol-5-yl) -N- [4- (2 , 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -lff-tetrazole-5- carboxamide ;

0170- (3- (amino-1, 2-benzisoxazol-5-yl) -N- [4- (2, 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] - 3- ( trifluoromethyl) -lff-1, 2 , 4-triazole-5-carboxamide;

0171- (3- (amino-1, 2-benzisoxazol-5-yl) -N- [4- (2 , 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -lff-1, 2,3- triazole-5-carboxamide;

0172- (3- (amino-1, 2-benzisoxazol-5-yl) -N- [4- (2, 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] - 2- (trifluoromethyl) -5-thiazolecarboxamide; and,

0173- (3- (amino-1, 2-benzisoxazol-5-yl) -N- [4- (2 , 3-dihydro-l, 1- dioxido-1, 2-benzisothiazol-7-yl)phenyl] -4, 5-dihydro-5- methyl-5-isoxazolecarboxamide; or a pharmaceutically acceptable salt form thereof.

0174[7] In a prefered embodiment, rings D-E combine to form a bicyclic moiety selected from the group:

0175<img file="WO0059902A2_D0018.tif" />

0176<img file="WO0059902A2_D0019.tif" /><img file="WO0059902A2_D0020.tif" />

0177<img file="WO0059902A2_D0021.tif" />

0178<img file="WO0059902A2_D0022.tif" />

0179In another embodiment, the present invention provides novel pharmaceutical compositions, comprising: a pharmaceutically acceptable carrier and a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt form thereof.

0180In another embodiment, the present invention provides a novel method for treating or preventing a thromboembolic disorder, comprising: administering to a patient in need thereof a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt form thereof . In another embodiment, the present invention provides novel compounds of formula (I) for use in therapy.

0181In another embodiment, the present invention provides the use of novel compounds of formula (I) for the manufacture of a medicament for the treatment of a thromboembolic disorder.

0182DEFINITIONS The compounds herein described may have asymmetric centers . Compounds of the present invention containing an asymmetrically substituted atom may be isolated in optically active or racemic forms. It is well known in the art how to prepare optically active forms, such as by resolution of racemic forms or by synthesis from optically active starting materials. Many geometric isomers of olefins, C=N double bonds, and the like can also be present in the compounds described herein, and all such stable isomers are contemplated in the present invention. Cis and trans geometric isomers of the compounds of the present invention are described and may be isolated as a mixture of isomers or as separated isomeric forms. All chiral, diastereomeric, racemic forms and all geometric isomeric forms of a structure are intended, unless the specific stereochemistry or isomeric form is specifically indicated. All processes used to prepare compounds of the present invention and intermediates made therein are considered to be part of the present invention.

0183The term "substituted, " as used herein, means that any one or more hydrogens on the designated atom is replaced with a selection from the indicated group, provided that the designated atom's normal valency is not exceeded, and that the substitution results in a stable compound. When a substitent is keto (i.e., =0), then 2 hydrogens on the atom are replaced. Keto substituents are not present on aromatic moieties .

0184The present invention is intended to include all isotopes of atoms occurring in the present compounds. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example and without limitation, isotopes of hydrogen include tritium and deuterium. Isotopes of carbon include C-13 and C-14.

0185When any variable (e.g., R^) occurs more than one time in any constituent or formula for a compound, its definition at each occurrence is independent of its definition at every other occurrence. Thus, for example, if a group is shown to be substituted with 0-2 R⁶, then said group may optionally be substituted with up to two R⁶ groups and R⁶ at each occurrence is selected independently from the definition of R⁶. Also, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds . When a bond to a substituent is shown to cross a bond connecting two atoms in a ring, then such substituent may be bonded to any atom on the ring. When a substituent is listed without indicating the atom via which such substituent is bonded to the rest of the compound of a given formula, then such substituent may be bonded via any atom in such substituent. Combinations of substituents and/or variables are permissible only if such combinations result in stable compounds.

0186As used herein, "alkyl" or "alkylene" is intended to include both branched and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms. Cι_ιo alkyl (or alkylene), is intended to include Ci, C₂, C₃, C , C₅, C₆, C₇, Cs, Cg, and Cι₀ alkyl groups. Examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, t-butyl, n-pentyl, and s-pentyl. "Haloalkyl" is intended to incliide both branched and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms, substituted with 1 or more halogen (for example -C_VF_W where v = 1 to 3 and w = 1 to (2v+l) ) . Examples of haloalkyl include, but are not limited to, trifluoromethyl, trichloromethyl, pentafluoroethyl, and pentachloroethyl . "Alkoxy" represents an alkyl group as defined above with the indicated number of carbon atoms attached through an oxygen bridge. Cι_ιo alkoxy, is intended to include Ci, C₂, C₃ , C₄, C₅, CQ , Cη , CQ , C₉, and C₁0 alkoxy groups. Examples of alkoxy include, but are not limited to, methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, s-butoxy, t-butoxy, n-pentoxy, and s-pentoxy. "Cycloalkyl" is intended to include saturated ring groups, such as cyclopropyl, cyclobutyl, or cyclopentyl. C₃-₇ cycloalkyl, is intended to include C₃ , C₄, C₅, e , and C₇ cycloalkyl groups. "Alkenyl" or "alkenylene" is intended to include hydrocarbon chains of either a straight or branched configuration and one or more unsaturated carbon-carbon bonds which may occur in any stable point along the chain, such as ethenyl and propenyl . C₂-ιo alkenyl (or alkenylene), is intended to include C₂, C₃ , C₄, C₅, CQ , Cη , Cs, Cg , and C₁₀ alkenyl groups. "Alkynyl" or "alkynylene" is intended to include hydrocarbon chains of either a straight or branched configuration and one or more triple carbon-carbon bonds which may occur in any stable point along the chain, such as ethynyl and propynyl . C₂-ιo alkynyl (or alkynylene), is intended to include C₂ , C₃ , C₄, C₅, C₆, C₇, C₈, C₉, and C₁0 alkynyl groups.

0187"Halo" or "halogen" as used herein refers to fluoro, chloro, bromo, and iodo; and "counterion" is used to represent a small, negatively charged species such as chloride, bromide, hydroxide, acetate, and sulfate.

0188As used herein, "carbocycle" or "carbocyclic residue" is intended to mean any stable 3, 4, 5, 6, or 7-membered monocyclic or bicyclic or 7, 8, 9, 10, 11, 12, or 13-membered bicyclic or tricyclic, any of which may be saturated, partially unsaturated, or aromatic. Examples of such carbocycles include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl , adamantyl , cyclooctyl , [3.3.0] bicyclooctane, [4.3.0]bicyclononane, [4.4.0]bicyclodecane,

0189[2.2.2]bicyclooctane, fluorenyl, phenyl, naphthyl, indanyl, adamantyl, and tetrahydronaphthyl .

0190As used herein, the term "heterocycle" or "heterocyclic system" is intended to mean a stable 5, 6, or 7-membered monocyclic or bicyclic or 7 , 8, 9, or 10-membered bicyclic heterocyclic ring which is saturated, partially unsaturated or unsaturated (aromatic) , and which consists of carbon atoms and 1, 2, 3, or 4 heteroatoms independently selected from the group consisting of N, NH, 0 and S and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring. The nitrogen and sulfur heteroatoms may optionally be oxidized. The heterocyclic ring may be attached to its pendant group at any heteroatom or carbon atom which results in a stable structure. The heterocyclic rings described herein may be substituted on carbon or on a nitrogen atom if the resulting compound is stable. A nitrogen in the heterocycle may optionally be quaternized. It is preferred that when the total number of S and O atoms in the heterocycle exceeds 1, then these heteroatoms are not adjacent to one another. It is preferred that the total number of S and 0 atoms in the heterocycle is not more than 1. As used herein, the term "aromatic heterocyclic system" or "heteroaryl" is intended to mean a stable 5, 6, or 7-membered monocyclic or bicyclic or 7 , 8, 9, or 10-membered bicyclic heterocyclic aromatic ring which consists of carbon atoms and 1, 2, 3, or 4 heterotams independently selected from the group consisting of N, NH, 0 and S. It is to be noted that total number of S and O atoms in the aromatic heterocycle is not more than 1. Examples of heterocycles include, but are not limited to, acridinyl, azocinyl, benzimidazolyl, benzofuranyl, benzothiofuranyl, benzothiophenyl, benzoxazolyl, benzthiazolyl, benztriazolyl, benztetrazolyl, benzisoxazolyl, benzisothiazolyl, benzimidazolinyl, carbazolyl, 4aff-carbazolyl, carbolinyl, chromanyl, chromenyl, cinnolinyl, decahydroquinolinyl, 2ff, 6H-1 , 5, 2- dithiazinyl, dihydrofuro [2 , 3 -b] tetrahydrofuran, furanyl, furazanyl, imidazolidinyl, imidazolinyl, imidazolyl, lff- indazolyl, indolenyl, indolinyl, indolizinyl, indolyl, 3H- indolyl, isobenzofuranyl, isochromanyl, isoindazolyl, isoindolinyl, isoindolyl, isoquinolinyl, isothiazolyl, isoxazolyl, methylenedioxyphenyl, morpholinyl, naphthyridinyl , octahydroisoquinolinyl, oxadiazolyl, 1,2,3- oxadiazolyl, 1, 2 , 4-oxadiazolyl, 1, 2 , 5-oxadiazolyl, 1,3,4- oxadiazolyl, oxazolidinyl, oxazolyl, oxazolidinyl, pyrimidinyl, phenanthridinyl , phenanthrolinyl, phenazinyl, phenothiazinyl, phenoxathiinyl, phenoxazinyl, phthalazinyl, piperazinyl, piperidinyl, piperidonyl , 4-piperidonyl, piperonyl , pteridinyl, purinyl, pyranyl, pyrazinyl, pyrazolidinyl, pyrazolinyl, pyrazolyl, pyridazinyl, pyridooxazole, pyridoimidazole, pyridothiazole, pyridinyl, pyridyl, pyrimidinyl, pyrrolidinyl, pyrrolinyl, 2H-pyrrolyl, pyrrolyl, quinazolinyl, quinolinyl, 4ff-quinolizinyl, quinoxalinyl, quinuclidinyl, tetrahydrofuranyl, tetrahydroisoquinolinyl, tetrahydroquinolinyl, tetrazolyl, 6ff-l,2, 5-thiadiazinyl, 1, 2 , 3-thiadiazolyl, 1,2,4- thiadiazolyl, 1, 2 , 5-thiadiazolyl, 1, 3 , 4-thiadiazolyl, thianthrenyl , thiazolyl, thienyl, thienothiazolyl, thienooxazolyl, thienoimidazolyl, thiophenyl, triazinyl, 1,2, 3-triazolyl, 1, 2 , 4-triazolyl, 1, 2 , 5-triazolyl, 1,3,4- triazolyl, and xanthenyl . Also included are fused ring and spiro compounds containing, for example, the above heterocycles . The phrase "pharmaceutically acceptable" is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio. As used herein, "pharmaceutically acceptable salts" refer to derivatives of the disclosed compounds wherein the parent compound is modified by making acid or base salts thereof. Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. The pharmaceutically acceptable salts include the conventional non-toxic salts or the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. For example, such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like; and the salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, and the like.

0191The pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington 's Pharmaceutical Sciences, 17th ed. , Mack Publishing Company, Easton, PA, 1985, p. 1418, the disclosure of which is hereby incorporated by reference.

0192Since prodrugs are known to enhance numerous desirable qualities of pharmaceuticals (e.g., solubility, bioavailability, manufacturing, etc ... ) the compounds of the present invention may be delivered in prodrug form. Thus, the present invention is intended to cover prodrugs of the presently claimed compounds, methods of delivering the same and compositions containing the same. "Prodrugs" are intended to include any covalently bonded carriers which release an active parent drug of the present invention in vivo when such prodrug is administered to a mammalian subject. Prodrugs the present invention are prepared by modifying functional groups present in the compound in such a way that the modifications are cleaved, either in routine manipulation or in vivo, to the parent compound. Prodrugs include compounds of the present invention wherein a hydroxy, amino, or sulfhydryl group is bonded to any group that, when the prodrug of the present invention is administered to a mammalian subject, it cleaves to form a free hydroxyl, free amino, or free sulfhydryl group, respectively. Examples of prodrugs include, but are not limited to, acetate, formate and benzoate derivatives of alcohol and amine functional groups in the compounds of the present invention. "Stable compound" and "stable structure" are meant to indicate a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent . "Substituted" is intended to indicate that one or more hydrogens on the atom indicated in the expression using "substituted" is replaced with a selection from the indicated group (s), provided that the indicated atom's normal valency is not exceeded, and that the substitution results in a stable compound. When a substituent is keto

0193(i.e., =0) group, then 2 hydrogens on the atom are replaced.

0194"Therapeutically effective amount" is intended to include an amount of a compound of the present invention or an amount of the combination of compounds claimed effective to treat the desired disease. The combination of compounds is preferably a synergistic combination. Synergy, as described for example by Chou and Talalay, Adv. Enzyme Regul. 22: 27-55 (1984), occurs when the effect of the compounds when administered in combination is greater than the additive effect of the compounds when administered alone as a single agent. In general, a synergistic effect is most clearly demonstrated at suboptimal concentrations of the compounds. Synergy can be in terms of lower cytotoxicity, increased therapeutic effect, or some other beneficial effect of the combination compared with the individual components .

0195SYNTHESIS The compounds of the present invention can be prepared in a number of ways known to one skilled in the art of organic synthesis. The compounds of the present invention can be synthesized using the methods described below, together with synthetic methods known in the art of synthetic organic chemistry, or by variations thereon as appreciated by those skilled in the art. Preferred methods include, but are not limited to, those described below. The reactions are performed in a solvent appropriate to the reagents and materials employed and suitable for the transformations being effected. It will be understood by those skilled in the art of organic synthesis that the functionality present on the molecule should be consistent with the transformations proposed. This will sometimes require a judgment to modify the order of the synthetic steps or to select one particular process scheme over another in order to obtain a desired compound of the invention. It will also be recognized that another major consideration in the planning of any synthetic route in this field is the judicious choice of the protecting group used for protection of the reactive functional groups present in the compounds described in this invention. An authoritative account describing the many alternatives to the trained practitioner is Greene and Wuts (Protective Groups In

0196Organic Synthesis, Wiley and Sons, 1991) . All references cited herein are hereby incorporated in their entirety herein by reference.

0197Scheme 1

0198<img file="WO0059902A2_D0023.tif" /> Scheme 1 presents how the A-B unit could be assembled, followed by the linker formation. Boronic acid is prepared according to the procedure by Pinto (WO 98/28269) and coupled with 5-bromo cyclic sulfone derivative (1) under Suzuki conditions. Deprotection of the aniline could be accomplished either with sodium borohydride in methanol or under standard basic conditions (lithium hydroxide in aqueous tetrahydrofuran) . Coupling to the core ring structures (I) could be accomplished according to the standard procedures known to the practitioners of the art. Scheme 2

0199<img file="WO0059902A2_D0024.tif" />

PDC

0201<img file="WO0059902A2_D0025.tif" />

0202Scheme 2 illustrates the preparation of 5-bromosultam derivatives. Conversion of the dibromoaniline to the dibromobenzenesulfonyl chloride can be accomplished according to the modified procedure by G. Wittig (Organic Synthesis, Coll. Vol .5 , 60), followed by the coupling with the appropriately substituted amine in the basic media. To achieve a metal-halogen exchange, the intermediate is treated with n-BuLi as shown by J. Wrobel (Heterocycles, Vol.38, 1823, 1994). The generated lithium anion could be quenched by the addition of N,N-dimethyl formamide. Deprotection and β-elimination can be achieved simultaneously under acidic conditions and is followed by the reduction of the imine bond with the appropriate reducing agent. Protection of the nitrogen of the final sultam derivative could be achieved with the number of the desired groups following the standard synthetic procedures commonly known in the art. Saccharine-like derivatives (3) could be obtained upon the treatment with an appropriate oxidizing agent, followed by the deprotection and introduction of the desired R₃ group.

0203Scheme 3

0204<img file="WO0059902A2_D0026.tif" />

0205Scheme 3 describes the synthesis of the desired azasultam derivative (4) by warming of the intermediate dibromophenylsulfonyl hydrazide in a basic media. Synthesis of the 6-ring aza-sultam (5) could proceed under halogen- metal exchange conditions described in Scheme 2 followed by the β-elimination and reduction to afford the desired compound.

0206Scheme 4

0207<img file="WO0059902A2_D0027.tif" />

0208In Scheme 4 dibromophenylsulfonyl chloride can be reduced to the corresponding sulfinic acid upon the treatment with sodium borohydride as described by A. Nose (Chem. Pharm. Bull. 35, 1770, 1987). The sulfinic acid undergoes Mannich condensation with formaldehyde and t-butyl amine as in A. Kanazawa (J. Org. Chem., 59, 1232, 1994) to afford an intermediate that undergoes halogen-metal exchange as was shown in Scheme 2 to give the 6-ring cyclic sulfone. Deprotection and β-elimination can be achieved simultaneously under acidic conditions and is followed by the reduction of the imine bond with the appropriate reducing agent as described in Scheme 2. Protection of the nitrogen of the final derivative (7) could be achieved with the number of the desired groups following the standard synthetic procedures commonly known in the art. Additionally, the intermediate aminomethyl sulfone can be cyclized to the desired 5-ring aza-sulfone (6) upon heating in the basic media. Scheme 5

0209<img file="WO0059902A2_D0028.tif" />

0210In Scheme 5 the sulfinic acid (Scheme 4) is treated with formaldehyde and disubstituted hydrazine to afford an intermediate that upon heating in the basic medium can cyclize to the final compound (8) .

0211Scheme 6

0212<img file="WO0059902A2_D0029.tif" />

0213Scheme 6 describes the synthesis of the desired 6-ring saccharin derivative (9) , where the dibromophenylsulfonylamide (Scheme 2) undergoes the palladium mediated coupling with TMS acetylene (Singh, J.

0214Org. Chem. 54, 4453, 1989) to provide the intermediate that is converted to the acid upon deprotection and treatment with the base as described by V. Laishev (Zh. Org. Khim.17, 2064, 1981) . Final amide bond formation could be accomplished using the standard conditions known in the art. Scheme 7

0215<img file="WO0059902A2_D0030.tif" />

021610

0217In Scheme 7 the treatment of the starting material under halogen-metal exchange conditions described in Scheme 2, followed by the quenching of the reaction medium with dibromoethane can affords the final 6-ring sultam derivative (10) .

0218Scheme 8

0219<img file="WO0059902A2_D0031.tif" />

022012

0221In Scheme 8 the reduction to the dibromophenylmethyl sulfone can be done according to the procedure by L. Field (Organic Synthesis, Coll. Vol. 4, 674). Halogen-metal exchange of this sulfone upon the treatment with n-BuLi according to the modified procedure by A. Cabidu, (Synthesis, 1, 41, 1993) followed by the quench with N,N- dimethyl formamide affords the cyclic 5-ring sulfone (11) after β-elimination and reduction of the intermediate imine. The treatment of the phenyl methylsulfone under similar halogen-metal exchange conditions and quenching with dibromoethane can provide the desired 6-ring cyclic sulfone (12) .

0222Scheme 9

0223<img file="WO0059902A2_D0032.tif" />

14 H

0225Scheme 9 describes the synthesis of 6-ring heterocyclic sulfone derivatives (13 and 14) . Synthesis of the dibromo sulfonamide is described in scheme 2. Animation is accomplished according to the procedure by A. Wisansky (Organic Synthesis, IV, 307, 1955). Treatment with phosgene can lead to the desired sulfonylcyclic urea derivative (13) . Additionally, treatment of the intermediate ammobromophenyl sulfonamide with N,N-dimethylformamide in the basic media, followed by the reduction of the imine leads to the 6-ring azasultam (14) .

0226Other features of the invention will become apparent in the course of the following descriptions of exemplary embodiments which are given for illustration of the invention and are not intended to be limiting thereof. EXAMPLES

0227Example 1

0228Synthesis of 2, 6-dibromobenzenesulfonyl chloride

0229<img file="WO0059902A2_D0033.tif" />

0230A solution of 2 , 6-dibromoaniline (10 g, 39.8 mmol) in trifluoroacetic acid (60 mL) and cone. HC1 (12 L) was cooled to 0°C in an ice bath and treated dropwise with a solution of sodium nitrate (3.4g, 39.8 mmol) in water (3 mL) over a period of 60 min. To the resulting mixture was added a suspension of copper (I) chloride (2.6 g) and copper (II) chloride (2.6 g) in sulfurous acid (70 mL) and acetic acid (70 mL) over a period of 90 min. After the addition was complete the resulting mixture was stirred at ambient temperature for 45 min. The resulting white precipitate was collected by filtration and dried under the reduced pressure to afford 2 , 6-dibromobenzenesulfonyl chloride as the white powder (5.2g, 39%). The title compound was used without further purification. ^XH NMR (CD₃OD) : δ 7.45 (t, 1H) , 8.01 (d, 2H) .

0231Example 2

0232Synthesis of 2, 6-dibromo-N- ( 1, 1-dimethylethyl) benzenesulfonamide

0233<img file="WO0059902A2_D0034.tif" />

0234A solution of 2 , 6-dibromobenzenesulfonyl chloride (4.7g, 14.0 mmol) in tetrahydrofuran (20 mL) was treated with potassium carbonate (5.8g, 42 mmol) and t-butyl amine (1.2 g, 16.8 mmol). The resulting mixture was stirred at ambient temperature over 18h then poured into water and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to afford 2,6- dibromo-N- (1, 1-dimethylethyl) benzenesulfonamide (4.5g, 87%) as the white solid, which was used without further purification. ¹H NMR (CD₃OD) : δ 1.22 (s, 9H) , 7.22 (t, 1H) ,

02357.85 (d, 2H) .

0236Example 3

0237Synthesis of 7 -bromo-2- (1, 1-dimethylethyl) -2, 3-dihydro-l, 2- benzisothiazol-3-ol 1, 1-dioxide

0238<img file="WO0059902A2_D0035.tif" />

0239To sodium hydride (0.52 g, 12.9 mmol) in tetrahydrofuran (20 mL) at 0°C was added 2 , 6-dibromo-N- (1, 1- dimethylethyl) benzenesulfonamide (4.0 g, 10.8 mmol). The resulting mixture was stirred at ambient temperature for 2-3 h, cooled to -78°C and treated with n-BuLi (1.6 M in hexanes, 7.4 mL, 11.9 mmol) over a period of 20 min. The reaction was continued to stir for 40 min and treated with N,N-dimethyl formamide (0.9 mL, 11.9 mL) . The cooling bath was removed and the mixture continued to stir over a period of 20 min. The work-up consisted of slowly quenching the reaction with IN NaCl, dilution with water and extraction with ethyl acetate. Drying over sodium sulfate and concentrating under reduced pressure provided the crude product that was purified by the flash chromatography (ethyl acetate/hexane, 1: 4) to afford the 7-bromo-2- (1, 1- dimethylethyl) -2 , 3-dihydro-l, 2-benzisothiazol-3-ol 1, 1- dioxide (2.1 g, 55 %).¹H NMR (CD₃OD) : δ 1.61 (s, 9H) , 6.02 (s, 1H) , 7.61 (m, 2H) , 7.77 (m, 1H) . Example 4 Synthesis of 7-bromo-2, 3-dihydro-l, 2-benzisothiazole 1,1- dioxide

0240<img file="WO0059902A2_D0036.tif" />

02417-bromo-2- (1, 1-dimethylethyl) -2 , 3-dihydro-l, 2- benzisothiazol-3-ol 1,1-dioxide (1.4g, 4.4 mmol) was treated with formic acid (10 mL) . The reaction was stirred at ambient temperature over 18h, concentrated in vacuo and dissolved in methanol (10 mL) . The mixture was cooled to

02420°C and treated with sodium borohydride (0.17 g, 4.4 mmol) in small portions over 20min. The cooling bath was removed and the resulting reaction mixture stirred at ambient temperature over 18h. The work-up consisted of slowly quenching the reaction mixture with ice, dilution with water and extraction with ethyl acetate. Drying over sodium sulfate and concentrating under reduced pressure provided the crude product that was purified by a plug of silica gel (ethyl acetate/hexane, 1: 4) to afford the desired 7-bromo- 2 , 3-dihydro-l, 2-benzisothiazole 1,1-dioxide (0.6 g, 60% over

02432 steps). H NMR (CD₃0D) : δ 4.40 (s, 2H) , 7.53 (m, 2H) , 7.70 (m, 1H) .

0244Example 5 Synthesis of 1, l-dimθthylethyl-7-bromo-l,2-bθnzisothiazole- 2 (3ff) -carboxylate 1,1-dioxide

0245<img file="WO0059902A2_D0037.tif" />

0246A solution of 7-bromo-2 , 3-dihydro-l, 2-benzisothiazole 1,1-dioxide (0.37 g, 1.5 mmol) in tetrahydrofuran (10 mL) was treated with DMAP (0.55 g, 4.5 mmol) and BOC anhydride (0.39 mg, 1.8 mmol). The resulting mixture was stirred at ambient temperature over 18h, then poured into water and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo . The residue was purified by a plug of silica gel (ethyl acetate/hexane, 1: 4) to afford 1, 1-dimethylethyl-7-bromo-1, 2- benzisothiazole-2 (3ff) -carboxylate 1,1-dioxide (0.5 g, 100%). ^λR NMR (CDC1₃) : δ 1.61 (s, 9H) , 4.78 (s, 2H) , 7.40 (d, 1H) , 7.54 (t, 1H) , 7.70 (d, 1H) .

0247Example 6

0248Synthesis of 7-bromo-iV,-V-diethyl-l,2-benzisothiazole-2 (3ff) - ethanamine 1,1-dioxide

0249<img file="WO0059902A2_D0038.tif" /> To sodium hydride (90 mg, 2.3 mmol) in tetrahydrofuran (10 mL) at 0°C was added 1, l-dimethylethyl-7-bromo-l, 2- benzisothiazole-2 (3H) -carboxylate 1,1-dioxide (0.36 g, 1.5 mmol) . The reaction was stirred for 20min and treated with N,N-diethylaminoethyl bromide hydrobromide (0.39 g, 1.5 mmol) and potassium carbonate (0.62 g, 4.5 mmol). The resulting mixture was stirred at ambient temperature over 18h, poured into ice water and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo . The crude material was purified by a flush chromatography (methanol/methylene chloride, 1: 9) to afford 7-bromo-N, N-diethyl -1, 2-benzisothiazole-2 (3ff) - ethanamine 1,1-dioxide (0.48 g, 93 %) . LRMS (ES+) : 348.0 (M+H)+. Example 7a Synthesis of 7-bromo-2-methyl-l,2-benzisothiazol-3-one 1,1- dioxide

0250<img file="WO0059902A2_D0039.tif" />

0251To a solution of 7-bromo-2-methyl-2 , 3-dihydro-l, 2- benzisothiazol-3-ol 1, 1-dioxide (0.34 g, 1.2 mmol) in methylene chloride (20 mL) , prepared according to the method described in Example 3 was added PDC (1.4 g, 3.6 mmol) . The reaction mixture is stirred at ambient temperature over 6h, filtered through a pad of Celite and concentrated under the reduced pressure. The residue was purified by flash chromatography (ethyl acetate/hexane, 1: 4) to afford the desired product 7-bromo-2-methyl-1, 2-benzisothiazol-3-one 1,1-dioxide (0.24 g, 72%). ²H NMR (CDC1₃) : δ 3.9 (s, 3H) ,

02527.71 (t, 1H) , 7.99 (d, 1H) , 8.03 (d, 1H) .

0253Example 7 Synthesis of 7-bromo-2,3-dihydro-2-methyl-l,2- benzisothiazole 1,1-dioxide

0254<img file="WO0059902A2_D0040.tif" />

0255To sodium hydride (40 mg, 1.0 mmol) in tetrahydrofuran (10 mL) at 0°C was added 7-bromo-N,N-diethyl-l, 2- benzisothiazole-2 (3H) -ethanamine 1,1-dioxide (0.26 g,1.0 mmol) . The reaction was stirred for 20 min and treated with methyl iodide (0.06 mL, 1.3 mmol). The resulting mixture was stirred at ambient temperature over 18h, poured into ice water and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo . The crude material was purified by a flush chromatography ((ethyl acetate/hexane, 1: 4) to afford 7-bromo-2,3- dihydro-2-methyl-1, 2-benzisothiazole 1,1-dioxide (0.15 g, 56 %) . LRMS (ES-) : 262.0 (M-H)⁺.

0256Example 8a

0257Synthesis of 1, l-dimethylethyl-7- (4- aminotrifluoroacetylphenyl) -1, 2-benzisothiazole-2 (3ff) - carboxylate 1,1-dioxide

0258<img file="WO0059902A2_D0041.tif" />

0259A solution of 1, l-dimethylethyl-7-bromo-l, 2- benzisothiazole-2 (3H) -carboxylate 1, 1-dioxide (0.58 g, 1.7 mmol) in dioxane (20 mL) was treated with potassium phosphate (0.71 g, 3.4 mmol) and 4-amino-trifluoroacetyl benzeneboronic acid (0.51 g,2.2 mmol). The resulting mixture was deoxygenated by a rapid stream of nitrogen applied to the system over a period of 20 min., then treated with Pd(0) at once. The reaction was refluxed over a period of 18 h, cooled down, filtered through Celite and washed with tetrahydrofuran (20 mL) . The filtrate evaporated to dryness, taken up in water and extracted with ethyl acetate (3x) . Ethyl acetate extracts were dried over sodium sulfate and concentrated. The crude residue was purified by flash chromatography (methanol/methylene chloride, 1: 9) to afford the desired product 1, l-dimethylethyl-7- (4- aminotrifluoroacetylphenyl) -1, 2-benzisothiazole-2 (3ff) - carboxylate 1,1-dioxide (0.68g, 67%). LRMS (ES+) : 479.2 (M+Na)\ Example 8

0260Synthesis of 1, l-dimethylethyl-7- (4-aminophenyl) -1,2- benzisothiazole-2 (3H) -carboxylate 1, 1-dioxide

0261<img file="WO0059902A2_D0042.tif" />

0262A solution of 1, l-dimethylethyl-7- (4- aminotrifluoroacetylphenyl) -1, 2-benzisothiazole-2 (3H) - carboxylate 1,1-dioxide (0.68 g, 1.5 mmol) in methanol (10 mL) at 0°C was treated with sodium borohydride (57 mg, 1.5 mmol) . The mixture was stirred over a period of 2 h, poured on ice and extracted with ethyl acetate, dried with sodium sulfate and concentrated. The residue was purified by flash chromatography (methanol/methylene chloride, 1: 9) to afford the desired product 1, l-dimethylethyl-7- (4- aminophenyl) -1, 2-benzisothiazole-2 (3ff) -carboxylate 1,1- dioxide (0.22g, 40%). LRMS (ES+) : 382.9 (M+Na)\

0263Example 9 Synthesis of 7- (4-aminobiphenyl) -2, 3-dihydro-2-methyl-1,2- benzisothiazol-3-ol 1,1-dioxide

0264<img file="WO0059902A2_D0043.tif" />

0265This compound was prepared by the method described in Example 8/8a starting with 7-bromo-2-methyl-1, 2- benzisothiazol-3-one 1,1-dioxide. LRMS (ES-) : 289.1 (M-H) Example 10

0266Synthesis of 7- (4-aminobiphenyl) -IV,JV-diethyl-1, 2- benzisothiazole-2 (3H) -ethanaminel, 1-dioxide

0267<img file="WO0059902A2_D0044.tif" />

0268This compound was prepared by the method described in Example 8/8a starting with 7-bromo-N, N-diethyl-1 , 2- benzisothiazole-2 (3ff) -ethanamine 1,1-dioxide. LRMS (ES+): 360.0 (M+H)\

0269Example 11 Synthesis of 7- (4-aminobiphenyl) -2, 3-dihydro-2-methyl-1,2- benzisothiazole 1,1-dioxide

0270<img file="WO0059902A2_D0045.tif" />

0271This compound was prepared by the method described in Example 8/8a starting with 7-bromo-2 , 3-dihydro-2-methyl-1, 2- benzisothiazole 1,1-dioxide. LRMS (ES+) m/z 393.1 (M+Νa)⁺.

0272Example 12

0273Synthesis of N- [4- (2, 3-dihydro-l, 1-dioxido-l,2- benzisothiazol-7-yl)phenyl] -1- (4-methoxyphenyl) -3-

0274(trifluoromethyl) -lff-pyrazole-5-carboxamide

0275<img file="WO0059902A2_D0046.tif" />

0276To the solution of 3- (trifluoromethyl) -1- (4- methoxyphenyl) -lff-pyrazolecarboxylic acid (0.1 g, 0.3 mmol) in dry acetonitrile (10 mL) was added thionyl chloride (0.15 mL, 1.8 mmol) . The reaction mixture was warmed up at 50 C for lh. The solvent and excess of thionyl chloride were removed under reduced pressure and dried on a vacuum pump over 18h. To this dried residue was added a mixture of 1,1- dimethylethyl-7- (4-aminophenyl) -1, 2-benzisothiazole-2 (3ff) - carboxylate 1,1-dioxide (0.1 g, 0.3 mmol) and DMAP (0.13 g, 1.8 mmol) in dry methylene chloride (10 L) . The reaction mixture was allowed to stir at ambient temperature for 18 h, then concentrated and purified by flash chromatography (ethyl acetate/hexane, 1: 1) to afford the desired product 1, l-dimethylethyl-7- [4- [ [ [1- (4-methoxyphenyl) -3-

0277(trifluoromethyl) -lff-pyrazol-5-yl] carbonyl] amino]phenyl] - 1, 2-benzisothiazole-2 (3ff-carboxylate) 1,2-dioxide (96 mg, 45%) LRMS (ES-) : 627.2 (M-H)⁺.

0278This product was treated with trifluoroacetic acid at ambient temperature over 2h to give the product that was purified by HPLC utilizing gradient elution with a mixture of water: acetonitrile and 0.05% of trifluoroacetic acid on a reverse phase C18 (60 angstrom) column to give IV- [4- (2,3- dihydro-1, 1-dioxido-l, 2-benzisothiazol-7-yl)phenyl] -1- (4- methoxyphenyl) -3- (trifluoromethyl) -lff-pyrazole-5- carboxamide. ^XH NMR (CD₃OD) : 63.81 (s, 3H) , 4.41 (s, 2H) , 7.02 (d, 2H, 7.29 (s, 1H) , 7.43 (m, 4H) , 7.67 (m, 5H) . Example 12a Synthesis of JV- [4- (1, 1-dioxido-l, 2-benzisothiazol-2- cyanomethy1-7-y1)phenyl] -1- (4-methoxyphenyl) -3- (trifluoromethyl) -lff-pyrazole-5-carboxamide

0279<img file="WO0059902A2_D0047.tif" />

0280To the solution of N- [4- (2, 3-dihydro-l, 1-dioxido-l, 2- benzisothiazol-7-yl) phenyl] -1- (4-methoxyphenyl) -3-

0281(trifluoromethyl) -lff-pyrazole-5-carboxamide (0.07 g, 0.13 mmol) in dry THF (10 mL) was added sodium hydride (0.011 g, 0.26 mmol) and iodoacetonitrile (0.01 mL, 0.13 mmol). The reaction mixture was stirred at ambient temperature over 18 hrs, then concentrated and purified by flash chromatography (ethyl acetate/hexane, 1: 1) to afford the desired product N- [4- (1, 1-dioxido-l, 2-benzisothiazol-2- cyanomethyl-7-yl) phenyl] -1- (4-methoxyphenyl) -3-

0282(trifluoromethyl) -lff-pyrazole-5-carboxamide LRMS (ES+) : 568.55 (M+H)\

0283Example 13

0284Synthesis of JV- [4- (2, 3-dihydro-3-hydroxy-l, 1-dioxido-l, 2- benzisothiazol-7-yl) phenyl] -1- (4-methoxyphenyl) -3-

0285( trif luoromethyl ) - lff-pyrazole- 5 -carboxamide

0286<img file="WO0059902A2_D0048.tif" /> This compound was prepared by the method described in Example 12 starting with 7- (4-aminobiphenyl) -2 , 3 -dihydro-2- methyl-1, 2-benzisothiazol-3-ol 1,1-dioxide. LRMS (ES-) : 557.1 (M-H)+.

0287Example 14 Synthesis of JV- [4- [2- [2- (diethylamino) ethyl] -2, 3-dihydro-

02881, 1-dioxido-l, 2-benzisothiazol-7-yl]phenyl] -1- (4- methoxyphenyl) -3- (trifluoromethyl) -lff-pyrazol-5-carboxamide

0289<img file="WO0059902A2_D0049.tif" />

0290This compound was prepared by the method described in Example 12 starting with 1, l-dimethylethyl-7- (4- aminophenyl) -1, 2-benzisothiazole-2 (3f) -carboxylate 1,1- dioxide. LRMS (ES+) : 628.2 (M+H)\

0291Example 15 Synthesis of 1- [3- (aminomethyl)phenyl] -N- [4- (2, 3-dihydro- 1, 1-dioxido-l, 2-benzisothiazol-7-yl)phenyl] -3- (trifluoromethyl) -lff-pyrazole-5-carboxamide

0292<img file="WO0059902A2_D0050.tif" /> To the solution of 3- (trifluoromethyl) -1- (3- cyanophenyl) -lff-pyrazolecarboxylic acid (0.1 g, 0.3 mmol) in dry acetonitrile (10 mL) was added thionyl chloride (0.15 mL, 1.8 mmol) . The reaction mixture was warmed up at 50 C for Ih. The solvent and excess of thionyl chloride were removed under reduced pressure and dried on a vacuum pump over 18h. To this dried resudue was added a mixture of 1,1- dimethylethyl-7- (4-aminophenyl) -1, 2-benzisothiazole-2 (3ff) - carboxylate 1,1-dioxide (0.1 g, 0.3 mmol) and DMAP (0.13 g, 1.8 mmol) in dry methylene chloride (10 mL) . The reaction mixture was allowed to stir at ambient temperature for 18 h, then concentrated and purified by flash chromatography (ethyl acetate/hexane, 1: 1) to afford the intermediate product . This material was reduced under an atmosphere of hydrogen gas (55 psi) in methanol (5 mL) and trifluoroacetic acid (5 mL) in the presence of 10% palladium on carbon catalyst (15 mg) over a period of 18h at ambient temperature. The reaction mixture was filtered over a pad of Celite and washed with methanol (5 mL x 3) . The residue was purified by HPLC utilizing gradient elution with a mixture of water: acetonitrile with 0.05% of trifluoroacetic acid on a reverse phase C18 (60 angstrom) column to give 1, 1-dimethylethyl 7-[4-[[[l-[3- (aminomethyl) phenyl] -3- (trifluoromethyl) -lff-pyrazol-5- yl] carbonyl] amino] phenyl ] -1, 2-benzisothiazole-2 (3ff) - carboxylate 1,1-dioxide (32 mg, 15%). LRMS (ES+) : 628.3 (M+H) ⁺ .

0293This product was treated with trifluoroacetic acid (5 mL) at ambient temperature over 2h to give the title compound. LRMS (ES+) : 528.2 (M+H)\ Example 16 Synthesis of 1- [3- (aminomethyl)phenyl] -JV- [4- (2, 3-dihydro-2- methyl-1, 1-dioxido-l, 2-benzisothiazol-7-yl)phenyl] -3- (trifluoromethyl) -lff-pyrazole-5-carboxamide

0294<img file="WO0059902A2_D0051.tif" />

0295This compound was prepared by the method described in Example 15 starting with 7- (4-aminobiphenyl) -2 , 3-dihydro-2 - methyl-1, 2-benzisothiazole 1,1-dioxide. LRMS (ES+) : 542.3 (M+H)\

0296Example 17 Synthesis of 1- [3- (aminomethyl) -4-fluorophenyl] -JV- [4- (2,3- dihydro-1, 1-dioxido-l, 2-benzisothiazol-7-yl)phenyl] -3- (trifluoromethyl) -lff-pyrazole-5-carboxamide

0297<img file="WO0059902A2_D0052.tif" />

0298This compound was prepared by the method described in Example 15 starting with 1 , l-dimethylethyl-7- (4- aminophenyl) -1, 2-benzisothiazole-2 (3ff) -carboxylate 1, 1- dioxide. LRMS (ES+) : 546.1 (M+Na)\ Example 18

0299Synthesis of 1- (3-amino-l, 2-benzisoxazol-5-yl)-JV- [4- (2,3- dihydro-1, 1-dioxido-l, 2-benzisothiazol-7-yl)phenyl] -3-

0300(trifluoromethyl) -lff-pyrazole-5-carboxamide

0301<img file="WO0059902A2_D0053.tif" />

0302To a solution of acetone oxime (74 mg, 1.0 mmol) in DMF (6 mL) was added sodium t-butoxide (1 M in THF, 1.0 mL) . The mixture was stirred at room temperature for half hour followed by addition of a solution of 1, 1-dimethylethyl 7- [4- [ [ [1- [4-fluoro-3-cyanophenyl] -3- (trifluoromethyl) -lff- pyrazol-5-yl] carbonyl] amino] phenyl] -1 , 2-benzisothiazole- 2 (3ff) -carboxylate 1,1-dioxide (0.22 g, 0.34 mmol) prepared according to Example 15 in DMF (4 mL) . The reaction was stirred at room temperature for 5 hours . The reaction mixture was then partitioned between ethyl acetate and HC1(5%), washed with HC1(5%), four times with water, brine, dried over sodium sulfate, filtered and concentrated. Flash chromatography (30% ethyl acetate/hexane) gave 100 mg of 1, 1-dimethylethyl 7- [4- [ [ [1- [4- isopropylideneaminooxy -3- cyanophenyl] -3- (trifluoromethyl) -lff-pyrazol-5- yl] carbonyl] amino] phenyl] -1, 2-benzisothiazole-2 (3ff) - carboxylate 1,1-dioxide. The intermediate (100 mg, 0.14 mmol) was dissolved in ethanol (4 mL) and to the solution was added HC1 (20%, 4 mL) . The reaction mixture was stirred at 80 »C for three hours. The reaction mixture was cooled to room temperature. The white precipitate was filtered to give the product that was purified by HPLC utilizing gradient elution with a mixture of water: acetonitrile with 0.05% of trifluoroacetic acid on a reverse phase C18 (60 angstrom) column to give the title compound (37 mg, 19%) . LRMS (ES+) 577.1 (M+Na)⁺.

0303Example 18

0304Synthesis of JV- [4- (2, 3-dihydro-2-cyanomethyl-1, 1-dioxido-

03051,2-benzisothiazol-7-yl)phenyl] -1- (4-methoxyphenyl) -3-

0306(trifluoromethyl) -lff-pyrazole-5-carboxamide

0307<img file="WO0059902A2_D0054.tif" /> To the solution of 1, 1-dimethylethyl 7-[4-[[[l-(4- methoxyphenyl) -3- (trifluoromethyl) -lff-pyrazol-5- yl] carbonyl] amino] phenyl] -1, 2-benzisothiazole-2 (3ff- carboxylate) 1,2-dioxide (0.07 g, 0.13 mmol) in dry tetrahydrofuran (10 mL) was added sodium hydride (60% in oil, 11 mg, 0.26 mmol) and iodoacetonitrile (0.001 mL, 0.13 mmol) . The reaction mixture was stirred at ambient temperature for 18 h. , poured into ice water and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo . This product was purified by HPLC utilizing gradient elution with a mixture of water: acetonitrile and 0.05% of trifluoroacetic acid on a reverse phase C18 (60 angstrom) column to give N-[4-(2,3- dihydro-2-cyanomethyl-1, 1-dioxido-l, 2-benzisothiazol-7- yl) phenyl] -1- (4-methoxyphenyl) -3- (trifluoromethyl) -1H- pyrazole-5-carboxamide. LRMS (ES+) : 590.2 (M+Na)⁺. The following tables contain representative examples of the present invention. Each entry in each table is intended to be paired with each formulae at the start of the table. For example, in Table 1, example 1 is intended to be paired with each of formulae a-bbbb and in Table 3, example 1 is intended to be paired with each of formulae a-bbbb.

0308The following groups are intended for group A in the following tables.

0309<img file="WO0059902A2_D0055.tif" />

03102-pyridyl 3-pyridyl 2-pyrimidyl

0311<img file="WO0059902A2_D0056.tif" /> -pyrimidyl -^{HN" ~B}

03122-CI-phenyl 2-F-phenyl

0313<img file="WO0059902A2_D0057.tif" /> 2,6-diF-phenyl

0314Table 1

0315<img file="WO0059902A2_D0058.tif" />

0316Z=C(0)NH Z=C(0)NH

0317<img file="WO0059902A2_D0059.tif" /> Z=C(0)NH Z=C(0)NH

0318<img file="WO0059902A2_D0060.tif" /><img file="WO0059902A2_D0061.tif" />

0319Z=C(0)CH₂ Z=C(0)CH₂

0320<img file="WO0059902A2_D0062.tif" />

0321Z=C(Q)CH₂ Z=C(0)CH₂

0322<img file="WO0059902A2_D0063.tif" /><img file="WO0059902A2_D0064.tif" />

0323<img file="WO0059902A2_D0065.tif" />

0324<img file="WO0059902A2_D0066.tif" />

0325<img file="WO0059902A2_D0067.tif" />

0326<img file="WO0059902A2_D0068.tif" />

032766

0328<img file="WO0059902A2_D0069.tif" />

0329A _Rla phenyl CH₃ phenyl CH₂CH₃ phenyl CF₃ phenyl SCH₃ phenyl SOCH₃ phenyl SO₂CH₃ phenyl CH₂NHS0₂CH₃ phenyl Cl phenyl F phenyl C0₂CH₃ phenyl CH₂OCH₃ phenyl C0₂NH₂ 2-pyridyl CH₃ 2-pyridyl CH₂CH₃ 2-pyridyl CF₃ 2-pyridyl SCH₃ 2-pyridyl SOCH₃ 2-pyridyl SO₂CH₃ 2-pyridyl CH₂NHS0 CH₃ 2-pyridyl Cl 2-pyridyl F

03302-pyridyl C0₂CH₃

03312-pyridyl CH₂OCH₃

03322-pyridyl C0₂NH₂

03333 -pyridyl CH₃

03343-pyridyl CH₂CH₃

03353-pyridyl CF₃

03363-pyridyl SCH₃

03373-pyridyl SOCH₃

03383 -pyridyl S0₂CH₃

03393 -pyridyl CH₂NHS0₂CH₃

03403 -pyridyl Cl

03413 -pyridyl F

03423 -pyridyl CO₂CH₃

03433 -pyridyl CH₂OCH₃

03443 -pyridyl C0₂NH₂

03452-pyrimidyl CH₃

03462-pyrimidyl CH₂CH₃

03472-pyrimidyl CF₃

03482-pyrimidyl SCH₃

03492-pyrimidyl SOCH₃

03502-pyrimidyl SO2CH3

03512-pyrimidyl CH₂NHS0₂CH₃

03522-pyrimidyl Cl

03532-pyrimidyl F

03542-pyrimidyl C0₂CH₃

03552-pyrimidyl CH₂OCH₃

03562-pyrimidyl C0₂NH₂

03575-pyrimidyl CH₃

03585-pyrimidyl CH₂CH₃

03595-pyrimidyl CF₃

03605-pyrimidyl SCH₃

03615-pyrimidyl SOCH₃

03625-pyrimidyl S0₂CH₃

03635-pyrimidyl CH₂NHS0₂CH₃

03645-pyrimidyl Cl

03655-pyrimidyl F

03665-pyrimidyl C0₂CH₃

03675-pyrimidyl CH₂OCH₃

03685-pyrimidyl C0₂NH₂

03692-Cl-phenyl CH₃

03702-Cl-phenyl CH₂CH₃

03712-Cl-phenyl CF₃

03722-Cl-phenyl SCH₃

03732-Cl-phenyl SOCH₃

03742-Cl-phenyl S0₂CH₃

03752-Cl-phenyl CH₂NHS0₂CH₃

03762-Cl-phenyl Cl

03772-Cl-phenyl F

03782-Cl-phenyl C0₂CH₃

03792-Cl-phenyl CH₂OCH₃72 2-Cl-phenyl C0₂NH₂

038073 2 -F -phenyl CH₃

038174 2-F-phenyl CH₂CH₃

038275 2 -F -phenyl CF₃

038376 2-F-phenyl SCH3

038477 2-F-phenyl SOCH3

038578 2-F-phenyl SO2CH3

038679 2-F-phenyl CH₂NHS0₂CH₃

038780 2-F-phenyl Cl

038881 2-F-phenyl F

038982 2-F-phenyl CO2CH3

039083 2-F-phenyl CH2OCH3

039184 2-F-phenyl C0₂NH₂

039285 2, 6-diF-phenyl CH₃

039386 2, 6-diF-phenyl CH₂CH₃

039487 2, 6-diF-phenyl CF₃

039588 2, 6-diF-phenyl SCH3

039689 2, 6-diF-phenyl SOCH3

039790 2, 6-diF-phenyl S0₂CH₃

039891 2, 6-diF-phenyl CH₂NHS0₂CH₃

039992 2, 6-diF-phenyl Cl

040093 2, 6-diF-phenyl F

040194 2, 6-diF-phenyl CO2CH3

040295 2, 6-diF-phenyl CH₂OCH₃

040396 2, 6-diF-phenyl C0₂NH₂

0404Table 2

0405<img file="WO0059902A2_D0070.tif" />

0406Z=C(0)NH Z=C(0)NH

0407<img file="WO0059902A2_D0071.tif" />

0408Z=C(0)NH Z=C(0)NH

0409<img file="WO0059902A2_D0072.tif" /><img file="WO0059902A2_D0073.tif" />

0410Z=C(0)CH₂ Z=C(Q)CH₂

0411<img file="WO0059902A2_D0074.tif" /> Z=C(0)CH₂ Z=C(0)CH₂

0412<img file="WO0059902A2_D0075.tif" />

0413<img file="WO0059902A2_D0076.tif" />

0414<img file="WO0059902A2_D0077.tif" />

0415<img file="WO0059902A2_D0078.tif" />

0416<img file="WO0059902A2_D0079.tif" />

0417<img file="WO0059902A2_D0080.tif" />

0418<img file="WO0059902A2_D0081.tif" />

0419Ex # A

04201 phenyl 2 2-pyridyl 3 2-pyrimidyl 4 5-pyrimidyl 5 2-Cl-phenyl 6 2-F-phenyl 7 2, 6-diF-phenyl

0421Utility The compounds of this invention are useful as anticoagulants for the treatment or prevention of thromboembolic disorders in mammals. The term "thromboembolic disorders" as used herein includes arterial or venous cardiovascular or cerebrovascular thromboembolic disorders, including, for example, unstable angina, first or recurrent myocardial infarction, ischemic sudden death, transient ischemic attack, stroke, atherosclerosis, venous thrombosis, deep vein thrombosis, thrombophlebitis, arterial embolism, coronary and cerebral arterial thrombosis, cerebral embolism, kidney embolisms, and pulmonary embolisms . The anticoagulant effect of compounds of the present invention is believed to be due to inhibition of factor Xa or thrombin.

0422The effectiveness of compounds of the present invention as inhibitors of factor Xa was determined using purified human factor Xa and synthetic substrate. The rate of factor Xa hydrolysis of chromogenic substrate S2222 (Kabi Pharmacia, Franklin, OH) was measured both in the absence and presence of compounds of the present invention. Hydrolysis of the substrate resulted in the release of pNA, which was monitored spectrophotometrically by measuring the increase in absorbance at 405 nM. A decrease in the rate of absorbance change at 405 nm in the presence of inhibitor is indicative of enzyme inhibition. The results of this assay are expressed as inhibitory constant, i .

0423Factor Xa determinations were made in 0.10 M sodium phosphate buffer, pH 7.5, containing 0.20 M NaCl, and 0.5 % PEG 8000. The Michaelis constant, K , for substrate hydrolysis was determined at 25°C using the method of Lineweaver and Burk. Values of Ki were determined by allowing 0.2-0.5 nM human factor Xa (Enzyme Research Laboratories, South Bend, IN) to react with the substrate (0.20 mM-1 mM) in the presence of inhibitor. Reactions were allowed to go for 30 minutes and the velocities (rate of absorbance change vs time) were measured in the time frame of 25-30 minutes. The following relationship was used to calculate Ki values:

0424(v₀-v_s)/v_s = I/(Ki (1 + S/K_m)) where : v₀ is the velocity of the control in the absence of inhibitor; v_s is the velocity in the presence of inhibitor; I is the concentration of inhibitor; Ki is the dissociation constant of the enzyme: inhibitor complex;

0425S is the concentration of substrate; K_m is the Michaelis constant. Using the methodology described above, a number of compounds of the present invention were found to exhibit a Ki of &lt;10 μM, thereby confirming the utility of the compounds of the present invention as effective Xa inhibitors.

0426The antithrombotic effect of compounds of the present invention can be demonstrated in a rabbit arterio-venous (AV) shunt thrombosis model. In this model, rabbits weighing 2-3 kg anesthetized with a mixture of xylazine (10 mg/kg i. .) and ketamine (50 mg/kg i.m.) are used. A saline-filled AV shunt device is connected between the femoral arterial and the femoral venous cannulae. The AV shunt device consists of a piece of 6-cm tygon tubing which contains a piece of silk thread. Blood will flow from the femoral artery via the AV-shunt into the femoral vein. The exposure of flowing blood to a silk thread will induce the formation of a significant thrombus. After forty minutes, the shunt is disconnected and the silk thread covered with thrombus is weighed. Test agents or vehicle will be given

0427(i.v., i.p., s.c, or orally) prior to the opening of the AV shunt. The percentage inhibition of thrombus formation is determined for each treatment group. The ID50 values (dose which produces 50% inhibition of thrombus formation) are estimated by linear regression.

0428The compounds of formula (I) may also be useful as inhibitors of serine proteases, notably human thrombin, plasma kallikrein and plasmin. Because of their inhibitory action, these compounds are indicated for use in the prevention or treatment of physiological reactions, blood coagulation and inflammation, catalyzed by the aforesaid class of enzymes. Specifically, the compounds have utility as drugs for the treatment of diseases arising from elevated thrombin activity such as myocardial infarction, and as reagents used as anticoagulants in the processing of blood to plasma for diagnostic and other commercial purposes. Some compounds of the present invention were shown to be direct acting inhibitors of the serine protease thrombin by their ability to inhibit the cleavage of small molecule substrates by thrombin in a purified system. In vi tro inhibition constants were determined by the method described by Kettner et al . in J^". Biol . Chem . 265, 18289-18297

0429(1990), herein incorporated by reference. In these assays, thrombin-mediated hydrolysis of the chromogenic substrate S2238 (Helena Laboratories, Beaumont, TX) was monitored spectrophotometrically. Addition of an inhibitor to the assay mixture results in decreased absorbance and is indicative of thrombin inhibition. Human thrombin (Enzyme Research Laboratories, Inc., South Bend, IN) at a concentration of 0.2 nM in 0.10 M sodium phosphate buffer, pH 7.5, 0.20 M NaCl, and 0.5% PEG 6000, was incubated with various substrate concentrations ranging from 0.20 to 0.02 mM. After 25 to 30 minutes of incubation, thrombin activity was assayed by monitoring the rate of increase in absorbance at 405 nm which arises owing to substrate hydrolysis. Inhibition constants were derived from reciprocal plots of the reaction velocity as a function of substrate concentration using the standard method of Lineweaver and Burk. Using the methodology described above, some compounds of this invention were evaluated and found to exhibit a K^ of less than 10 μm, thereby confirming the utility of the compounds of the present invention as effective thrombin inhibitors . Compounds tested in the above assay are considered to be active if they exhibit a Ki of &lt;10 μM. Preferred compounds of the present invention have Ki ' s of &lt;1 uM. More preferred compounds of the present invention have Ki ' s of &lt;0.1 μM. Even more preferred compounds of the present invention have Ki's of &lt;0.01 μM. Still more preferred compounds of the present invention have Ki's of &lt;0.001 μM.

0430The compounds of the present invention can be administered alone or in combination with one or more additional therapeutic agents. These include other anticoagulant or coagulation inhibitory agents, anti-platelet or platelet inhibitory agents, thrombin inhibitors, or thrombolytic or fibrinolytic agents .

0431The compounds are administered to a mammal in a therapeutically effective amount. By "therapeutically effective amount" it is meant an amount of a compound of Formula I that, when administered alone or in combination with an additional therapeutic agent to a mammal, is effective to prevent or ameliorate the thromboembolic disease condition or the progression of the disease.

0432By "administered in combination" or "combination therapy" it is meant that the compound of Formula I and one or more additional therapeutic agents are administered concurrently to the mammal being treated. When administered in combination each component may be administered at the same time or sequentially in any order at different points in time. Thus, each component may be administered separately but sufficiently closely in time so as to provide the desired therapeutic effect. Other anticoagulant agents (or coagulation inhibitory agents) that may be used in combination with the compounds of this invention include warfarin and heparin, as well as other factor Xa inhibitors such as those described in the publications identified above under Background of the Invention. The term anti-platelet agents (or platelet inhibito'ry agents) , as used herein, denotes agents that inhibit platelet function such as by inhibiting the aggregation, adhesion or granular secretion of platelets. Such agents include, but are not limited to, the various known non-steroidal anti-inflammatory drugs (NSAIDS) such as aspirin, ibuprofen, naproxen, sulindac, indomethacin, mefenamate, droxicam, diclofenac, sulfinpyrazone, and piroxicam, including pharmaceutically acceptable salts or prodrugs thereof. Of the NSAIDS, aspirin (acetylsalicyclic acid or ASA) , and piroxicam are preferred. Other suitable anti-platelet agents include ticlopidine, including pharmaceutically acceptable salts or prodrugs thereof.

0433Ticlopidine is also a preferred compound since it is known to be gentle on the gastro-intestinal tract in use. Still other suitable platelet inhibitory agents include Ilb/IIIa antagonists, thromboxane-A2-receptor antagonists and thromboxane-A2-synthetase inhibitors, as well as pharmaceutically acceptable salts or prodrugs thereof.

0434The term thrombin inhibitors (or anti-thrombin agents) , as used herein, denotes inhibitors of the serine protease thrombin. By inhibiting thrombin, various thrombin-mediated processes, such as thrombin-mediated platelet activation

0435(that is, for example, the aggregation of platelets, and/or the granular secretion of plasminogen activator inhibitor-1 and/or serotonin) and/or fibrin formation are disrupted. A number of thrombin inhibitors are known to one of skill in the art and these inhibitors are contemplated to be used in combination with the present compounds . Such inhibitors include, but are not limited to, boroarginine derivatives, boropeptides, heparins, hirudin and argatroban, including pharmaceutically acceptable salts and prodrugs thereof. Boroarginine derivatives and boropeptides include N-acetyl and peptide derivatives of boronic acid, such as C-terminal a-aminoboronic acid derivatives of lysine, ornithine, arginine, homoarginine and corresponding isothiouronium analogs thereof. The term hirudin, as used herein, includes suitable derivatives or analogs of hirudin, referred to herein as hirulogs, such as disulfatohirudin. Boropeptide thrombin inhibitors include compounds described in Kettner et al., U.S. Patent No. 5,187,157 and European Patent Application Publication Number 293 881 A2 , the disclosures of which are hereby incorporated herein by reference. Other suitable boroarginine derivatives and boropeptide thrombin inhibitors include those disclosed in PCT Application

0436Publication Number 92/07869 and European Patent Application Publication Number 471,651 A2 , the disclosures of which are hereby incorporated herein by reference.

0437The term thrombolytics (or fibrinolytic) agents (or thrombolytics or fibrinolytics) , as used herein, denotes agents that lyse blood clots (thrombi) . Such agents include tissue plasminogen activator, anistreplase, urokinase or streptokinase, including pharmaceutically acceptable salts or prodrugs thereof. The term anistreplase, as used herein, refers to anisoylated plasminogen streptokinase activator complex, as described, for example, in European Patent Application No. 028,489, the disclosure of which is hereby incorporated herein by reference herein. The term urokinase, as used herein, is intended to denote both dual and single chain urokinase, the latter also being referred to herein as prourokinase .

0438Administration of the compounds of Formula I of the invention in combination with such additional therapeutic agent, may afford an efficacy advantage over the compounds and agents alone, and may do so while permitting the use of lower doses of each. A lower dosage minimizes the potential of side effects, thereby providing an increased margin of safety.

0439The compounds of the present invention are also useful as standard or reference compounds, for example as a quality standard or control, in tests or assays involving the inhibition of factor Xa. Such compounds may be provided in a commercial kit, for example, for use in pharmaceutical research involving factor Xa. For example, a compound of the present invention could be used as a reference in an assay to compare its known activity to a compound with an unknown activity. This would ensure the experimenter that the assay was being performed properly and provide a basis for comparison, especially if the test compound was a derivative of the reference compound. When developing new assays or protocols, compounds according to the present invention could be used to test their effectiveness.

0440The compounds of the present invention may also be used in diagnostic assays involving factor Xa. For example, the presence of factor Xa in an unknown sample could be determined by addition of chromogenic substrate S2222 to a series of solutions containing test sample and optionally one of the compounds of the present invention. If production of pNA is observed in the solutions containing test sample, but not in the presence of a compound of the present invention, then one would conclude factor Xa was present.

0441Dosage and Formulation The compounds of this invention can be administered in such oral dosage forms as tablets, capsules (each of which includes sustained release or timed release formulations) , pills, powders, granules, elixirs, tinctures, suspensions, syrups, and emulsions. They may also be administered in intravenous (bolus or infusion) , intraperitoneal, subcutaneous, or intramuscular form, all using dosage forms well known to those of ordinary skill in the pharmaceutical arts. They can be administered alone, but generally will be administered with a pharmaceutical carrier selected on the basis of the chosen route of administration and standard pharmaceutical practice. The dosage regimen for the compounds of the present invention will, of course, vary depending upon known factors, such as the pharmacodynamic characteristics of the particular agent and its mode and route of administration; the species, age, sex, health, medical condition, and weight of the recipient; the nature and extent of the symptoms; the kind of concurrent treatment; the frequency of treatment; the route of administration, the renal and hepatic function of the patient, and the effect desired. A physician or veterinarian can determine and prescribe the effective amount of the drug required to prevent, counter, or arrest the progress of the thromboembolic disorder. By way of general guidance, the daily oral dosage of each active ingredient, when used for the indicated effects, will range between about 0.001 to 1000 mg/kg of body weight, preferably between about 0.01 to 100 mg/kg of body weight per day, and most preferably between about 1.0 to 20 mg/kg/day. Intravenously, the most preferred doses will range from about 1 to about 10 mg/kg/minute during a constant rate infusion. Compounds of this invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three, or four times daily.

0442Compounds of this invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using transdermal skin patches . When administered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen .

0443The compounds are typically administered in admixture with suitable pharmaceutical diluents, excipients, or carriers (collectively referred to herein as pharmaceutical carriers) suitably selected with respect to the intended form of administration, that is, oral tablets, capsules, elixirs, syrups and the like, and consistent with conventional pharmaceutical practices . For instance, for oral administration in the form of a tablet or capsule, the active drug component can be combined with an oral, non-toxic, pharmaceutically acceptable, inert carrier such as lactose, starch, sucrose, glucose, methyl callulose, magnesium stearate, dicalcium phosphate, calcium sulfate, mannitol, sorbitol and the like; for oral administration in liquid form, the oral drug components can be combined with any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents, and coloring agents can also be incorporated into the mixture. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth, or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes, and the like. Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrators include, without limitation, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like. The compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles, and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine, or phosphatidylcholines .

0444Compounds of the present invention may also be coupled with soluble polymers as targetable drug carriers . Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxyethylaspartamidephenol, or polyethyleneoxide- polylysine substituted with palmitoyl residues. Furthermore, the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyglycolic acid, copolymers of polylactic and polyglycolic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacylates, and crosslinked or amphipathic block copolymers of hydrogels. Dosage forms (pharmaceutical compositions) suitable for administration may contain from about 1 milligram to about 100 milligrams of active ingredient per dosage unit. In these pharmaceutical compositions the active ingredient will ordinarily be present in an amount of about 0.5-95% by weight based on the total weight of the composition.

0445Gelatin capsules may contain the active ingredient and powdered carriers, such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. Similar diluents can be used to make compressed tablets. Both tablets and capsules can be manufactured as sustained release products to provide for continuous release of medication over a period of hours. Compressed tablets can be sugar coated or film coated to mask any unpleasant taste and protect the tablet from the atmosphere, or enteric coated for selective disintegration in the gastrointestinal tract . Liquid dosage forms for oral administration can contain coloring and flavoring to increase patient acceptance.

0446In general, water, a suitable oil, saline, aqueous dextrose (glucose) , and related sugar solutions and glycols such as propylene glycol or polyethylene glycols are suitable carriers for parenteral solutions. Solutions for parenteral administration preferably contain a water soluble salt of the active ingredient, suitable stabilizing agents, and if necessary, buffer substances. Antioxidizing agents such as sodium bisulfite, sodium sulfite, or ascorbic acid, either alone or combined, are suitable stabilizing agents.

0447Also used are citric acid and its salts and sodium EDTA. In addition, parenteral solutions can contain preservatives, such as benzalkonium chloride, methyl- or propyl-paraben, and chlorobutanol . Suitable pharmaceutical carriers are described in

0448Remington's Pharmaceutical Sciences. Mack Publishing Company, a standard reference text in this field.

0449Representative useful pharmaceutical dosage-forms for administration of the compounds of this invention can be illustrated as follows: Capsules A large number of unit capsules can be prepared by filling standard two-piece hard gelatin capsules each with 100 milligrams of powdered active ingredient, 150 milligrams of lactose, 50 milligrams of cellulose, and 6 milligrams magnesium stearate.

0450Soft Gelatin Capsules

0451A mixture of active ingredient in a digestable oil such as soybean oil, cottonseed oil or olive oil may be prepared and injected by means of a positive displacement pump into gelatin to form soft gelatin capsules containing 100 milligrams of the active ingredient. The capsules should be washed and dried. Tablets

0452Tablets may be prepared by conventional procedures so that the dosage unit is 100 milligrams of active ingredient, 0.2 milligrams of colloidal silicon dioxide, 5 milligrams of magnesium stearate, 275 milligrams of microcrystalline cellulose, 11 milligrams of starch and 98.8 milligrams of lactose. Appropriate coatings may be applied to increase palatability or delay absorption.

0453Injectable

0454A parenteral composition suitable for administration by injection may be prepared by stirring 1.5% by weight of active ingredient in 10% by volume propylene glycol and water. The solution should be made isotonic with sodium chloride and sterilized. Suspension

0455An aqueous suspension can be prepared for oral administration so that each 5 mL contain 100 mg of finely divided active ingredient, 200 mg of sodium carboxymethyl cellulose, 5 mg of sodium benzoate, 1.0 g of sorbitol solution, U.S. P., and 0.025 L of vanillin.

0456Where the compounds of this invention are combined with other anticoagulant agents, for example, a daily dosage may be about 0.1 to 100 milligrams of the compound of Formula I and about 1 to 7.5 milligrams of the second anticoagulant, per kilogram of patient body weight. For a tablet dosage form, the compounds of this invention generally may be present in an amount of about 5 to 10 milligrams per dosage unit, and the second anti-coagulant in an amount of about 1 to 5 milligrams per dosage unit. Where the compounds of Formula I are administered in combination with an anti-platelet agent, by way of general guidance, typically a daily dosage may be about 0.01 to 25 milligrams of the compound of Formula I and about 50 to 150 milligrams of the anti-platelet agent, preferably about 0.1 to 1 milligrams of the compound of Formula I and about 1 to 3 milligrams of antiplatelet agents, per kilogram of patient body weight .

0457Where the compounds of Formula I are adminstered in combination with thrombolytic agent, typically a daily dosage may be about 0.1 to 1 milligrams of the compound of Formula I, per kilogram of patient body weight and, in the case of the thrombolytic agents, the usual dosage of the thrombolyic agent when administered alone may be reduced by about 70-80% when administered with a compound of Formula I. Where two or more of the foregoing second therapeutic agents are administered with the compound of Formula I, generally the amount of each component in a typical daily dosage and typical dosage form may be reduced relative to the usual dosage of the agent when administered alone, in view of the additive or synergistic effect of the therapeutic agents when administered in combination.

0458Particularly when provided as a single dosage unit, the potential exists for a chemical interaction between the combined active ingredients. For this reason, when the compound of Formula I and a second therapeutic agent are combined in a single dosage unit they are formulated such that although the active ingredients are combined in a single dosage unit, the physical contact between the active ingredients is minimized (that is, reduced) . For example, one active ingredient may be enteric coated. By enteric coating one of the active ingredients, it is possible not only to minimize the contact between the combined active ingredients, but also, it is possible to control the release of one of these components in the gastrointestinal tract such that one of these components is not released in the stomach but rather is released in the intestines. One of the active ingredients may also be coated with a material which effects a sustained-release throughout the gastrointestinal tract and also serves to minimize physical contact between the combined active ingredients. Furthermore, the sustained-released component can be additionally enteric coated such that the release of this component occurs only in the intestine. Still another approach would involve the formulation of a combination product in which the one component is coated with a sustained and/or enteric release polymer, and the other component is also coated with a polymer such as a low- viscosity grade of hydroxypropyl methylcellulose (HPMC) or other appropriate materials as known in the art, in order to further separate the active components. The polymer coating serves to form an additional barrier to interaction with the other component.

0459These as well as other ways of minimizing contact between the components of combination products of the present invention, whether administered in a single dosage form or administered in separate forms but at the same time by the same manner, will be readily apparent to those skilled in the art, once armed with the present disclosure.

0460Obviously, numerous modifications and variations of the present invention are possible in light of the above teachings . It is therefore to be understood that within the scope of the appended claims, the invention may be practiced otherwise that as specifically described herein.