TW494239B

A method of controlling magnetic material attracting/releasing making use of a pipette device with a magnet body and analyzers using the method

Abstract

A magnetic material attraction/release control method used to suck liquid out of the container and release the liquid to the pipette device of the container. One or more magnets are arranged on the liquid suction line of the pipette device, and any magnetic material contained in the liquid attracted to the liquid suction line is deposited on the inner surface of the line by magnetic force. When the influence of the magnetic force generated by the magnet is cut off, the magnetic material loosens and is released from the liquid suction line together with the liquid.

TW494239B, drawing sheet 1 — Sheet 1 of 9

Term

No projected expiry on record.

55 claims: 51 independent, 4 dependent

1
A magnetic material attraction/release control method using a pipette device with one magnet or multiple magnets, wherein the magnetic system is detachable and installed on the outer peripheral surface of the liquid suction line of the pipette device to remove the liquid To suck and release the liquid from the container, the pipette device provides control by attracting and maintaining the magnetic material, which is contained in the liquid, and is located on the inner surface of the liquid suction line due to one or more magnets. The magnetism of the magnet is attracted to the liquid suction line, and at the same time, the magnetic material is released from the liquid suction line due to the interference of the magnetism in one or more magnets, so that the magnetic material is released into the liquid together with the liquid Absorb the outer surface of the wire, where the magnetic material is formed into granules and can be combined with the target material. 1.一種使用具有一磁體或多磁體之吸量管裝置之磁性材料吸引／釋放控制方法，其中此磁體係可取下之安裝在該吸量管裝置之液體吸取線之外部周圍表面，以便將液體從容器中吸出並釋放液體，吸量管裝置藉由吸引和維持此磁性材料而提供控制，其中此磁性材料包含於該液體之中，並因位於液體吸取線之內表面上之一磁體或多磁體之磁性而被吸引至液體吸取線，且其同時亦藉由於一磁體或多磁體中之磁性的干擾影響，而從液體吸取線釋放此磁性材料，而使得磁性材料與液體一起被釋放至液體吸取線之外面，其中此磁性材料係形成粒狀並能夠與目標材料結合。
2
For example, the magnetic material attraction/release control method using a pipette device in the first item of the scope of patent application, in which a plurality of parallel liquid suction lines are arranged, and the liquid suction line is used to suck or release the liquid operation The drive and control of the magnetic materials can be simultaneously attracted and released at the same time, respectively. 2.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中設置多個互相平行之液體吸取線，且在液體吸取線中之用於吸取或釋放液體之操作的驅動和控制使磁性材料可分別地被同時吸引，且被同時釋放。
3
For example, the magnetic material attraction/release control method using a pipette device in the first item of the scope of patent application, in which a plurality of liquid suction lines are arranged parallel to each other, and each liquid suction line is driven and controlled so that each liquid is sucked The liquid in the line is respectively attracted or released at different time periods, and a special procedure is passed to each liquid suction line to attract or release the magnetic material separately. 3.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中設置多個互相平行之液體吸取線，且每一個液體吸取線之驅動和控制使每一個液體吸取線中之液體在不同的時段被分別地吸引或釋放，且經由一特殊之程序至每一個液體吸取線以便分別地吸引或釋放磁性材料。
4
For example, the magnetic material attraction/release control method using a pipette device in the first item of the patent application, in which the liquid suction line and a magnet or multiple magnets are integrated into a unit, and multiple units are arranged along the container transfer line set up. 4.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中液體吸取線與一磁體或多磁體被整合成一單元，而多個單元則沿著容器轉移線而設置。
5
For example, the magnetic material attraction/release control method using a pipette device in the first item of the scope of patent application, wherein the magnetic system in one or more units is set on the liquid suction line. 5.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中一個或多個單元中之磁體係設於液體吸取線上。
6
For example, in the first item of the scope of patent application, the magnetic material attraction/release control method using pipette device, one of the magnets or multi-magnetic system is set outside the liquid suction line. 6.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中一磁體或多磁體係設於液體吸取線外。
7
For example, the magnetic material attraction/release control method using pipette device in the first item of the scope of patent application, in which one magnet or multiple magnets are installed on the liquid suction line. 7.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中一磁體或多磁體被安裝於液體吸取線上。
8
For example, the magnetic material attraction/release control method using a pipette device in the first item of the scope of patent application, wherein the magnet includes a permanent magnet, and the magnetic substance contained in the liquid and attracted by the liquid suction line, by making the magnet gradually Close to the liquid suction line, it is adsorbed and deposited on the inner surface of the liquid suction line, and the magnetic material and the liquid are released outside the liquid suction line. The magnetic material and the liquid are absorbed by gradually moving the magnet away from the liquid suction line Line separation. 8.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中磁體包括永久磁鐵，包含於液體中且被液體吸取線吸引之磁性物質，藉由使磁體漸漸的接近液體吸取線而吸附並沉積於液體吸取線之內表面，且磁性材料與液體一起被釋放至液體吸取線之外，其乃藉由使磁體漸漸的遠離液體吸取線而將磁性材料與液體吸取線分離。
9
The magnetic material attraction/release control method using a pipette device as claimed in the first item of the patent application, wherein the magnet includes an electromagnet, and the magnetic material contained in the liquid and attracted by the liquid suction line is generated by electromagnetism The magnetic force is attracted and maintained on the inner surface of the liquid suction line. When the magnetic force generated by the electromagnetic disappears or is completely reduced, the magnetic material separates from the liquid suction line and is released together with the liquid. 9.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中磁體包括電磁鐵，包含於液體中且被液體吸取線吸引之該磁性材料乃藉由電磁所產生之磁力被吸引並維持於該液體吸取線之內表面，當此由電磁所產生之磁力消失或完全地減少時，磁性材料與液體吸取線分離並與液體一起被釋放。
10
The magnetic material attraction/release control method using a pipette device as claimed in the first item of the scope of patent application, wherein the pipette chip is detachably mounted on the tip area of the liquid suction side of the liquid suction line to form liquid The wire is sucked, and the magnetic force generated by the electromagnetic can affect the magnetic material stored in the liquid in the pipette chip. 10.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中吸量管芯片係可拆裝式地安裝於液體吸取線之液體吸取側之尖端區域以便形成液體吸取線，且由電磁所產生之磁力可影響儲存於吸量管芯片之液體中之磁性材料。
11
For example, the magnetic material attraction/release control method using a pipette device in the scope of the patent application, wherein the pipette chip is transferred according to each inspection method when the magnetic material is attracted and maintained on its inner surface To the special processing location. 11.如申請專利範圍第10項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中吸量管芯片在當磁性材料被吸引並維持於其內表面時，依據每個檢驗方法被轉移至特殊處理位置。
12
The magnetic material attraction/release control method using pipette device as claimed in item 10 of the scope of patent application, wherein the pipette chip is repeatedly used for the same sample according to the special inspection method required by the sample. 12.如申請專利範圍第10項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中吸量管芯片，依據樣品所要求之特殊檢驗方法而被重複地使用於相同樣品。
13
For example, the magnetic material attraction/release control method using a pipette device in the scope of the patent application, in which the operation for separating, stirring and cleaning the magnetic material from the liquid containing it is within the liquid suction line The inner and outer surfaces of the liquid contact zone are performed by repeatedly sucking and releasing liquid to the extent that cross-contamination does not occur. 13.如申請專利範圍第10項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中用於將磁性材料從包含其之液體中分離、攪拌和清潔之操作是在液體吸取線內之液體接觸區之內和外表面藉由重複地吸取和釋放液體至交互污染不會發生之程度之後進行。
14
For example, the magnetic material attraction/release control method using a pipette device in the first item of the patent application, in which the separation, stirring, and cleaning of the magnetic material from the liquid containing it are performed by the liquid suction line once or Achieved by multiple operations of sucking and releasing liquid. 14.如申請專利範圍第1項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中將磁性材料從包含其之液體中分離、攪拌、清潔的執行，是藉由液體吸取線一次或多次的吸取和釋放液體之操作而達成。
15
For example, the method for controlling the attraction/release of magnetic material using pipette device in the scope of patent application, in which the separation of the magnetic material from the liquid containing it is performed by when the magnetic material is continuously attracted by the magnet At this time, only the liquid is released. 15.如申請專利範圍第14項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中將磁性材料從包含其之液體中分離之執行，是藉由在當磁性材料持續的被磁體吸引時，僅釋放液體而達成。
16
For example, the magnetic material attraction/release control method using a pipette device in the scope of the patent application, in which the stirring is performed when the pipette chip has been inserted into the liquid stored in other containers, and the magnetic material is After the magnet is attracted to the inner surface of the pipette chip, it is achieved by repeatedly sucking and releasing the aforementioned liquid until the aforementioned operation is not affected by the magnetic force generated by the magnet. 16.如申請專利範圍第14項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中攪拌之執行，係在當吸量管芯片已插入儲存於其它容器之液體中，且磁性材料被磁體吸引在該吸量管芯片之內表面之後，藉由重複地吸取和釋放前述的液體，直到前述的操作不受磁鐵所產生之磁力的影響而達成。
17
For example, the magnetic material attraction/release control method using a pipette device in the 14th patent application, in which cleaning is performed when the pipette chip has been transferred to a clean position and the magnetic material is attracted by the magnet. After measuring the inner surface of the chip, it is achieved by repeatedly attracting and releasing the cleaning liquid. 17.如申請專利範圍第14項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中清潔之執行，係當吸量管芯片已被轉移至清潔的位置且磁性材料被磁體吸引在吸量管芯片之內表面之後，藉由重複地吸引和釋放清洗液而達成。
18
For example, the magnetic material attraction/release control method using a pipette device in the scope of the patent application, in which the cleaning with a cleaning solution is performed when the magnetic material is attracted to the inner surface of the pipette chip . 18.如申請專利範圍第14項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中以清洗液所作之清潔，是在當磁性材料被吸引在吸量管芯片之內表面上時執行。
19
For example, the magnetic material attraction/release control method using a pipette device in the scope of the patent application, in which the cleaning performed by the cleaning liquid is performed by attracting and releasing the cleaning liquid one or more times until the operation It is not affected by the magnetic force generated by the magnet. 19.如申請專利範圍第18項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中以清洗液所作之清潔之執行，是藉由一次或多次的吸引和釋放清洗液，直到操作不受磁體所產生之磁力的影響而達成。
20
For example, the magnetic material attraction/release control method using a pipette device in the scope of the patent application, wherein the separation, stirring, and cleaning of the magnetic material from the liquid contained therein are performed by attracting and releasing the previously stored It is achieved by the liquid in each liquid storage area, which is set up in a box with one or more liquid storage areas in it according to requirements. 20.如申請專利範圍第14項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中將磁性材料從包含其中之液體中分離、攪拌、清潔之執行，是藉由吸引和釋放先前儲存於每個液體儲存區中之液體而達成，此儲存區是設於依據所需而具有其中設有一個或多個液體儲存區之盒者。
21
For example, the magnetic material attraction/release control method using a pipette device in the 14th patent application, in which the separation, stirring, and cleaning of the magnetic material from the liquid containing it are performed by removing the remaining liquid from the container The medium is released to the outside of the container with the pipette chip with the magnetic material deposited on the inner surface, and then the liquid required for the next procedure is poured into the same container, and the poured liquid is sucked and released by the pipette chip . 21.如申請專利範圍第14項之使用吸量管裝置之磁性材料吸引／釋放控制方法，其中將磁性材料從包含其之液體中分離、攪拌、清潔之執行，是藉由將剩餘液體從容器中釋放至具有內表面沉積有磁性材料的吸量管芯片之容器之外，然後將下一個程序所需之液體倒至相同之容器中，並以吸量管芯片吸取和釋放此倒入之液體。
22
A magnetic material attraction/release control method using a pipette device, wherein the amount of liquid sucked by the liquid suction line is controlled to a specific level, and each time the liquid is sucked and released by the pipette device, it The qualitative and quantitative analysis of the target material can be performed with high accuracy. 22.一種使用吸量管裝置之磁性材料吸引／釋放控制方法，其中將液體吸取線吸取之液體量控制於一特定的位準，且每一次以吸量管裝置吸取和釋放該液體時，其目標材料之定性和定量之分析可被高精確地執行。
23
An analyzer that uses the magnetic material attraction/release control method of the pipette device, which will be based on any one of the 1st to the 22nd item of the patent application for the magnetic material attraction/release of the pipette device The release control method, which is applied to clinical testing methods, includes:a container;a pipette device, including a first area, the first area is inserted into the vessel;a second area, the diameter of the second area is larger than the diameter of the second area The diameter of the first zone;and the third zone, the diameter of the third zone is greater than the diameter of the second zone;the pipette device is used to transfer the mixed liquid and magnetic material to be tested from the container to the pipette device The third zone;the magnetic material becomes granular and can be combined with the target material;a mechanism is attached to the third zone to transfer liquid from the container to the pipette, or transfer liquid from the pipette Into the container;a magnet device, arranged on the outside of the pipette device, for supplying a magnetic field to the second area, the magnet device periodically supplying a magnetic field to gather magnetic materials on the inner wall of the second area, and When the magnetic field is removed, the magnetic material is transferred to the container, and the pipette device installed can controllably suck or release the liquid through the second zone into the third zone or enter the third zone from the third zone. The second district. 23.一種利用吸量管裝置之磁性材料吸引／釋放控制方法之分析器，其係將依據申請專利範圍第1項至第22項中之任一項之使用吸量管裝置之磁性材料吸引／釋放控制方法，應用於臨床的檢驗方法上，包括：容器；吸量管裝置，包括第一區，該第一區插入於該溶器內；第二區，該第二區之直徑要大於第一區之直徑；和第三區，該第三區之直徑要大於第二區之直徑；該吸量管裝置用來將被檢驗之混合液體和磁性材料從容器轉移至吸量管裝置之該第三區；該磁性材料成為粒狀，能夠與目標材料結合；有一機構附接到該第三區，可將液體從容器內轉移至該吸量管內，或將液體從吸量管內轉移至該容器內；磁鐵裝置，設於吸量管裝置之外面，用於供應磁場至該第二區，該磁鐵裝置周期地供應磁場以便將磁性材料聚集於該第二區之內牆上，而於移離此磁場時使此磁性材料轉移至此容器中，和該裝設之吸量管裝置可控制地分別吸取或釋放液體經過該第二區進入該第三區或從該第三區進入該第二區。
24
For example, the analyzer of item 23 of the scope of patent application, in which the program executed by the analyzer is based on the chemical immunoassay method. 24.如申請專利範圍第23項之分析器，其中分析器所執行之程序是以化學免疫檢驗方法為基礎。
25
For example, the analyzer of item 24 of the scope of patent application, in which the container is made into a card-like shape with multiple liquid storage areas, and liquids and samples are poured into each liquid storage according to the reaction or procedure. The container with the magnetic material deposited on the inner surface of the liquid suction line by the magnetic force generated by the magnet is transferable. 25.如申請專利範圍第24項之分析器，其中容器被做成一具有多個液體儲存區之卡式狀之形狀，將液體和樣品依據反應或程序之所須先行倒入每個液體儲存區中，且具有藉由磁體所產生之磁力而沉積於液體吸取線之內表面的磁性材料之容器係可轉移者。
26
Such as the analyzer of item 24 of the scope of patent application, in which the container is made into a micro-disc shape arranged with several liquid storage areas. 26.如申請專利範圍第24項之分析器，其中容器被做成設置排列有數個液體儲存區之微盤狀者。
27
A method of using a pipette device to analyze a target material contained in a liquid body. The pipette device has a liquid suction line including a liquid inlet end for sucking a liquid containing a magnetic material from a container to the suction volume The water storage tank of the pipe device and the release of the liquid through the liquid inlet end, and the external peripheral surface of the liquid suction line of the pipette device with magnets or multi-magnets that can be removed, including the following steps:(a) The liquid body and the granular magnetic material are mixed in the container to form a magnetic mixture that can be combined with the target material;(b) transfer the magnetic mixture from the container to the water storage tank of the pipette device;(c) repeatedly A magnetic field is applied to and away from the water storage tank to process the magnetic material;(d) the magnetic material is transferred from the water storage tank to the container to produce a test mixture;(e) thereafter, the test mixture is tested to analyze the target material. 27.一種使用吸量管裝置分析包含於液體本體內之目標材料之方法，此吸量管裝置具有液體吸取線包括液體入口端，用來從容器中吸取包含有磁性材料之液體至該吸量管裝置之貯水槽，並經由該液體入口端釋放該液體，和具有磁體或多磁體可卸下之安裝在該吸量管裝置液體吸取線之外部周圍表面，包括下列步驟：(a)將該液體本體和成為粒狀之磁性材料混合於容器內以便形成磁性混合物，並能夠與目標材料結合；(b)將此磁性混合物從容器轉移至吸量管裝置之貯水槽；(c)重複地將磁場施加至和遠離貯水槽以便處理此磁性材料；(d)將此磁性材料從貯水槽轉移至容器以便產生測試混合物；(e)其後，測試此測試混合物以便分析目標材料。
28
The method according to item 27 of the scope of patent application, wherein step (a) includes transferring the first predetermined amount of liquid body from the first container to the water storage tank of the pipette device. 28.如申請專利範圍第27項之方法，其中步驟(a)包括從第一容器轉移第一事先決定量之液體本體至吸量管裝置之貯水槽。
31
Such as the method of item 27 of the scope of patent application, wherein step (c) includes repeatedly transferring the magnetic mixture from the pipette device to the container, and then from the container to the pipette device, so that the magnetic mixture can be stirred. Magnetic mixture until the magnetic material has been homogeneously mixed with the liquid body. 31.如申請專利範圍第27項之方法，其中步驟(c)又包括重覆地將此磁性混合物從吸量管裝置轉移至容器中，再從容器轉移至吸量管裝置，以便可攪拌此磁性混合物直至此磁性材料已均質地與液體本體混合。
32
The method according to item 31 of the scope of patent application, wherein when a magnetic field is applied to the pipette device, the magnetic mixture is transferred from the pipette device to the container and then from the container to the pipette device. 32.如申請專利範圍第31項之方法，其中在當磁場施於吸量管裝置時，將此磁性混合物從吸量管裝置轉移至容器中再從容器轉移至吸量管裝置。
33
Such as the method of item 32 of the scope of patent application, wherein the magnet is arranged outside the pipette device so as to generate a magnetic field. 33.如申請專利範圍第32項之方法，其中磁鐵係設於吸量管裝置之外，以便可產生磁場。
34
Such as the method of item 32 of the scope of patent application, wherein the magnetic field is used to gather magnetic materials on the inner wall of the water storage tank. 34.如申請專利範圍第32項之方法，其中此磁場係用於將磁性材料聚集於貯水槽之內牆。
35
The method of item 32 of the scope of the patent application, wherein step (c) also includes releasing the liquid body into the container when the magnetic material is accumulated on the inner wall of the water storage tank. 35.如申請專利範圍第32項之方法，其中步驟(c)亦包括在當磁性材料被聚集於貯水槽之內牆時，其將液體本體釋放至容器中。
36
The method as claimed in item 35 of the scope of patent application, wherein step (c) also includes transferring the cleaning liquid from the third container to the water storage tank after releasing the liquid body. 36.如申請專利範圍第35項之方法，其中步驟(c)亦包括當釋放此液體本體之後，將清洗液從第三容器轉移至貯水槽。
37
Such as the method of item 36 of the scope of patent application, wherein step (c) also includes removing the magnetic field after the cleaning liquid is transferred to the water storage tank. 37.如申請專利範圍第36項之方法，其中步驟(c)亦包括當清洗液被轉移至貯水槽之後，將該磁場移離。
38
Such as the method of item 37 of the scope of patent application, wherein step (c) also includes when the magnetic field is removed, by transferring the cleaning liquid and magnetic material from the third container to the water storage tank and then from the water storage tank to the second Three containers while cleaning this magnetic material. 38.如申請專利範圍第37項之方法，其中步驟(c)亦包括當此磁場被移離時，藉由將清洗液和磁性材料從第三容器轉移至貯水槽再從貯水槽轉移至第三容器，而清潔此磁性材料。
39
The method as claimed in item 38 of the scope of patent application, wherein a magnetic field is applied after the cleaning step to collect the magnetic material on the inner wall of the water storage tank and release the cleaning liquid. 39.如申請專利範圍第38項之方法，其中在清潔步驟之後施以磁場以便將磁性材料聚集於貯水槽之內牆上，並釋放該清洗液。
40
Such as the method of item 39 in the scope of patent application, wherein the cleaning step is repeated at least once. 40.如申請專利範圍第39項之方法，其中清潔步驟重覆至少一次以上。
42
Such as the method of item 41 in the scope of patent application, wherein step (c) also includes when the magnetic field is removed, by transferring the marking liquid and magnetic material from the fourth container to the water storage tank and then from the water storage tank to the first Four containers, and mark this magnetic material. 42.如申請專利範圍第41項之方法，其中步驟(c)亦包括當此磁場被移離時，藉由將標記液體和磁性材料從第四容器轉至貯水槽再從貯水槽轉移至第四容器，而標記此磁性材料。
43
The method as claimed in item 42 of the scope of patent application, wherein the magnetic field is applied after the marking step to gather the magnetic material on the inner wall of the water storage tank and release the marking liquid. 43.如申請專利範圍第42項之方法，其中在標記步驟之後施加該磁場，以便將磁性材料聚集於貯水槽之內牆上，並將標記液體釋放。
45
The method according to item 44 of the scope of patent application, wherein step (d) includes transferring the matrix liquid from the fifth container to the water storage tank when a magnetic field is applied. 45.如申請專利範圍第44項之方法，其中步驟(d)包括當施加磁場時，將基質液從第五容器轉移至貯水槽。
46
Such as the method of item 45 in the scope of the patent application, wherein step (d) also includes when the magnetic field is removed, releasing the matrix liquid and the magnetic material from the water storage tank to the fifth container so as to produce the test mixture in the fifth container . 46.如申請專利範圍第45項之方法，其中步驟(d)亦包括當此磁場被移離時，將基質液和磁性材料從貯水槽釋放至第五容器以便於第五容器中產生測試混合物。
47
The method according to item 27 of the scope of patent application, wherein step (e) includes measuring the light emitted by the test liquid. 47.如申請專利範圍第27項之方法，其中步驟(e)包括測量由測試液體所發射之光。
48
The method according to item 47 of the scope of patent application, wherein the matrix liquid includes a light emission trigger liquid. 48.如申請專利範圍第47項之方法，其中基質液包括光發射觸發液體。
49
The method according to item 47 of the scope of patent application, wherein the matrix liquid includes hydrogen peroxide. 49.如申請專利範圍第47項之方法，其中基質液包括過氧化氫。
50
The method according to item 46 of the scope of patent application, wherein the container includes the aforementioned first, second, third, fourth and fifth containers. 50.如申請專利範圍第46項之方法，其中容器包括前述的第一、第二、第三，第四及第五容器。
51
A device for chemiluminescence inspection, comprising:a container;a pipette device, comprising a first zone, the first zone is inserted into the vessel;a second zone, the diameter of the second zone is larger than the first zone The diameter of the area;and the third area, the diameter of the third area is greater than the diameter of the second area;the pipette device is used to transfer the mixed liquid and magnetic material to be tested from the container to the pipette device Three zones;the magnetic material becomes granular and can be combined with the target material;there is a mechanism attached to the third zone, which can transfer liquid from the container to the pipette, or transfer liquid from the pipette to In the container;a magnet device, arranged on the outside of the pipette device, for supplying a magnetic field to the second area, the magnet device periodically supplying a magnetic field to gather magnetic materials on the inner wall of the second area, and When the magnetic field is removed, the magnetic material is transferred to the container, and the pipette device installed can controllably suck or release the liquid through the second zone and enter the third zone, or enter the third zone from the third zone. The second area. 51.一種用於化學發光檢驗之裝置，包括：容器；吸量管裝置，包括第一區，該第一區插入於該溶器內；第二區，該第二區之直徑要大於第一區之直徑；和第三區，該第三區之直徑要大於第二區之直徑；該吸量管裝置用來將被檢驗之混合液體和磁性材料從容器轉移至吸量管裝置之該第三區；該磁性材料成為粒狀，能夠與目標材料結合；有一機構附接到該第三區，可將液體從容器內轉移至該吸量管內，或將液體從吸量管內轉移至該容器內；磁鐵裝置，設於吸量管裝置之外面，用於供應磁場至該第二區，該磁鐵裝置周期地供應磁場以便將磁性材料聚集於該第二區之內牆上，而於移離此磁場時使此磁性材料轉移至此容器中，和該裝設之吸量管裝置可控制地分別吸取或釋放該液體經過該第二區進入該第三區，或從該第三區進入該第二區。
52
Such as the device of item 51 of the scope of patent application, wherein the water storage tank includes a first area inserted into the container, a second area having a larger diameter than the first area, and a third area having a larger diameter than the second area. 52.如申請專利範圍第51項之裝置，其中貯水槽包括插入容器之第一區，具有直徑較一區大之第二區，及具有直徑較第二區大之第三區。
53
Such as the device of item 52 of the scope of patent application, wherein the magnet device is arranged near the second area of the water storage tank so as to gather magnetic materials on the inner wall of the second area. 53.如申請專利範圍第52項之裝置，其中磁鐵裝置設於貯水槽之第二區的附近，以便將磁性材料聚集於第二區之內牆上。
54
Such as the device of item 53 of the scope of patent application, wherein the pipette device also includes a mechanism for transferring liquid to and from the container. 54.如申請專利範圍第53項之裝置，其中吸量管裝置又包括用於將液體轉移至容器和從容器轉移之機構。
55
Such as the device of item 54 of the scope of patent application, wherein the mechanism is connected to the third area of the water storage tank. 55.如申請專利範圍第54項之裝置，其中該機構連接於貯水槽之第三區。

Independent claims51

127 paragraphs, as filed

Method for controlling attraction/release of magnetic material by pipette device with magnet and analyzer using this method

P. . . Pipette chipM. . . magnetx. . . UndersideP1. . . Liquid suction linePA. . . Low-end area1. . . Sample reaction container2. . . Magnetic material3. . . Reaction insoluble magnetic material liquid5. . . Cleaning fluid6. . . Enzyme-labeled liquid7. . . Matrix fluid8. . . Reaction stop solution9. . . (PMT) optical measuring instrument10. . . Finest area11. . . Mid-diameter area12. . . Large diameter area1A to 1H. . . Liquid storage area16. . . filter17. . . nozzle20. . . Absorbent pad

Fig. 1 shows a processing flow chart when the present invention is applied to an example of a chemical immunoassay method based on a chemiluminescence method.

Figure 2a is a cross-sectional view showing an example of the pipette chip used in the present invention.

Figure 2b is a more realistic cross-sectional view of the pipette chip P in Figure 2a.

Figure 3 is an explanatory diagram showing the general structure of the measurement part when the present invention is applied to the chemical immunoassay method according to the CLEIA method.

Figure 4 is an explanatory diagram showing the general structure of the measurement part in an example of the chemical immunoassay method according to the CLIA method when the present invention is applied.

Figure 5 is an explanatory diagram showing the general structure of the measurement part when the present invention is applied to an example of a chemical immunoassay method based on the EIA method.

Figure 6 is an explanatory diagram showing the arrangement of magnets when the liquid suction line of the present invention is a nozzle system.

Fig. 7 is an explanatory diagram showing the arrangement of magnets in another example of the present invention.

Fig. 8 is an explanatory diagram showing the arrangement of magnets in another example of the present invention.

Figure 9 shows the processing flow chart of the traditional chemiluminescence method of chemical immunoassay.

Field of invention

The present invention relates to a novel magnetic material attraction/release control method, which can capture or diffuse magnetic materials. The present invention also relates to various types of analyzers made using this method.

It should be understood that, as defined in this specification, "magnetic material" does not only refer to a spherical material, but can also be a particle or particulate material, and its shape is not limited to a spherical shape, but can be Any shape.

Background of the invention

In recent years, various chemiluminescence methods (CL methods) have been developed. These methods include, for example, enzyme immunoassay (EIA) using antigen-antibody reaction, and chemiluminescence immunoassay in a narrow sense. (CLIA), and in this chemiluminescence immunoassay, chemiluminescence compounds are used as markers for immunoassay tracers, and chemiluminescence enzyme immunoassay (CLEIA), which uses chemiluminescence compounds in the detection system Detect enzyme activity with high sensitivity.

When using any of the above-mentioned inspection methods, there is a known magnetic particle method. This method uses magnetic particles coated with antigen or antibody on the surface of each particle: latex method, which uses a method that uses antigen or antibody coated on the surface of each particle. Latex: Melt bead method, which uses spherical beads coated with antigen or antibody on its surface: or so-called tube coating method, which uses a cell with antigen or antibody coated on the inner wall. However, when considering the efficiency of extracting antigens or antibodies, as well as production costs and operating costs, the use of magnetic bodies such as magnetic particles or the method of molten beads has more advantages.

However, in the conventional inspection method using the magnetic material as described above, it is necessary to clean the magnetic material or gather the magnetic material floating or deposited in the reactor such as the test product reaction vessel, so that the magnetic material is A reagent reacts or causes it to float several times in the reactor. However, it is extremely difficult to maintain the aggregation or stirring of the magnetic material with high precision in this procedure, and this is why it is used One of the reasons why the inspection method of magnetic materials has not been automated in various applications.

The above-mentioned chemical immunization procedure using magnetic materials will be described with reference to the flow chart in Figure 9. In this process, in step (a), first sample the required number of samples in container 1 by pipette device P1, and in step (b), by second pipette device P2, The reaction-insoluble magnetic liquid is poured into container 1. In step (c), a vibrating stirrer is used for stirring, in step (d), culture is performed (at a fixed temperature), and in step (e), the attraction of the magnetic material and the liquid by the magnet M are performed.Its out. The discharge. Then in step (f), the cleaning liquid is poured into the container through the pipette device P3.

Then in step (g), stirring is performed by shaking the stirrer. In step (h), when the cleaning solution is discharged, the magnetic material 2 is attracted by the magnet M, and in step (i), the fourth suction volume The tube device P4 pours the labeling liquid 6 into it. In step (j), stir by shaking the stirrer. In step (k), the reaction conditions for culture and fixation are carried out, and then in step (1), when this is discharged The reaction liquid is attracted to the magnetic material by the magnet M, and in step (m), the cleaning liquid is poured through the fifth pipette device P5. Then, in step (n), stirring is performed by shaking the stirrer.

Then, for example, in the CLEIA method, in step (o), when the cleaning liquid is discharged, the magnetic material 2 is attracted by the magnet M, in step (p), the carrier liquid is poured in, in step (q) , Stir by shaking the stirrer, and then in step (r), the sample is kept for a period of time, and in step (s), the amount of light emitted from the reaction system is measured by an optical measuring machine such as PMT.

On the other hand, in the example of the CLIEA method, after the above step (n), in step (t), the cleaning liquid containing the magnetic material 2 in the container 1 is sucked out, and the cleaning liquid is poured into The measuring cell of the filter, and the magnetic material 2 contained in the cleaning liquid is collected by the filter. Then, in step (u), hydrogen peroxide (H 2 O 2 ) The liquid is poured into the magnetic material 2 collected by the aforementioned filter to convert the liquid to emit light, and the amount of this emitted light is measured by the PMT which strictly prevents light from the outside.

On the other hand, in a detection method like the CLEIA method or the EIA method, in step (s), the light emission continues for a period of time after the matrix solution is poured, and in step (t), the reaction The amount of light generated in the slab is most often measured by optical measuring instruments such as PMT.

The above describes the traditional detection method using magnetic materials, but it can be clearly understood from the foregoing that the traditional detection method uses the above-mentioned magnetic material, which needs to be attracted to the inner wall of the container The magnetic material, and then the attracted magnetic material is uniformly diffused into the liquid many times. It is extremely difficult to perform the separation, stirring and cleaning of the magnetic material and the liquid with high precision, and this is a problem to be solved.

In other words, when the magnetic material is separated from the liquid, in the traditional type of detection method, the general magnetic attraction occurs on the side wall of the large container. The magnetic material is attracted to the inner wall of the container. The efficiency of collecting this magnetic material is therefore very low.

Moreover, when the magnetic material is gathered on the inner surface of the container and the pipette is inserted into the liquid to absorb the liquid, the magnetic material will be attracted along with the liquid. Therefore, it will be necessary to completely capture the magnetic material. Extremely difficult.

Furthermore, when stirring the liquid with the magnetic material diffused in it, the magnetism in the magnet is usually eliminated, and mixing and dispersing the magnetic material once attracted to the liquid in the container usually uses vibration. However, it is difficult to uniformly diffuse the magnetic material in the liquid, and sometimes the liquid mixed with the magnetic material will splash on the surface of the container, which is another problem to be solved. Therefore, when vibrating stirring is used in the traditional technique, the liquid containing the magnetic material splashed on the upper surface of the container must be washed away. Therefore, the procedure becomes more complicated, and if the operation of washing off the liquid is not performed completely, the subsequent steps of the procedure will be seriously affected.

Furthermore, when cleaning the liquid and magnetic materials in the container as described above, the substances deposited on the surface of the magnetic material during the above-mentioned separation process and stirring process are removed, but the same problem occurs in the separation and stirring process. Will also happen.

From time to time, in the detection method using traditional magnetic materials, if a reaction procedure or processing procedure is very special, it is necessary to establish a mechanism for separation, stirring, and cleaning, and a suitable method for this special procedure. Control System. Therefore, this mechanism or control system will become very complicated, and in fact it is impossible to perform detection using this magnetic material based on a very special reaction or processing procedure. As a result, the equipment or operating costs will become very high.

In addition, in the method of gathering magnetic materials based on the aforementioned traditional technology, it is difficult to place the above-mentioned magnet in a container such as a microplate, and even if possible, place the magnet in the container. The side is also difficult. It is also difficult to perform separation, stirring and anti-cleaning by sucking out the magnetic material from the liquid. Therefore, it is extremely difficult to shrink the container by using the microdisk, and it is also a fatal disadvantage.

Summary of the invention

An object of the present invention is to provide a novel pipette (pipette) for magnetic material attraction/release control method. Its most notable feature is that it captures and gathers the magnetic material diffused in the liquid from the liquid. It is executed on the side of the container containing the sample, but executed in the suction volume that can attract and release the liquid containing the magnetic material. This pipette can significantly improve the detection method using this type of magnetic material, that is, the magnetism of the magnet on the attracting/releasing side of the pipette chip, etc. installed in the pipette device, in a very short time It will be completely attracted within time. If it uses a disposable pipette chip, cross contamination can be avoided, and this method can be easily applied to various detections based on special reactions or processing procedures, respectively. According to the present invention, a detection device using magnetic materials can be produced. The device is simple in terms of its structure and operation, and is also versatile and low-cost.

In order to achieve the above-mentioned object, in the method for controlling the attraction/release of magnetic material using a pipette according to the present invention, a magnet is provided in the liquid suction line of the pipette that attracts and releases liquid from the inside of the container. Any magnetic material attracted by the liquid suction line due to the magnetism of the magnet is attracted and retained on the inner surface of the liquid suction line, and then the magnetic material is separated from the liquid suction line and released from the liquid suction line together with the liquid.

In the present invention, in order to enhance the processing capacity, a plurality of liquid suction lines parallel to each other are provided. The actuation and control of the introduction or release of the liquid in each liquid suction line is to enable the attraction or separation of the magnetic material in the liquid to be performed at the same time. In this way, a multi-channel system that allows multiple samples to be processed at the same time can be realized.

Furthermore, in the present invention, it is possible to provide a plurality of liquid suction lines as described above in order to increase the processing capacity to respond to any liquid that requires a special processing procedure. Each liquid suction line is independently controlled at a different time sequence, so the liquid suction and release can be separately controlled through a special processing program to attract or separate the mixed and diffused magnetic materials.

In the present invention, only at least one liquid suction line as described above is required. The improvement of the processing capacity can be achieved by integrating the liquid suction line and the magnetic material into a unit and providing a plurality of units as described above along the container conversion line.

At the same time, in the present invention, the above-mentioned magnet includes any type of permanent magnet or electromagnet, as long as it can generate magnetism in order to attract the magnetic material, and one or more magnets will be The quantity and size of the attracted magnetic material are set on each liquid suction line. Various modes for placing the magnet can be considered. For example, the magnet can be placed in the liquid flow direction of the liquid suction line or in the opposite direction on both sides of the liquid suction line, or in the axial ray direction.

Furthermore, in the present invention, the above-mentioned magnet can be placed on the outside of the liquid suction line or directly on the liquid suction line.

When the magnet is placed on the outer side of the liquid suction line as described above, by using multiple permanent magnets as the above-mentioned magnet and placing the magnetic end on or near the liquid suction line, it may attract and retain the inclusion The magnetic material in the liquid attracted to the liquid suction line is on the inner surface of the liquid suction line. It is also possible to release the magnetic material and liquid from the liquid suction line by moving the magnet away from the liquid suction line to separate the magnetic material from the line.

When the magnet is directly placed on or near the liquid suction line, by forming an electromagnet magnet and generating magnetism in the electromagnetic domain, it can attract and maintain the magnetic material contained in the liquid attracted to the liquid suction line Above the inside of the liquid suction line. It is also possible to separate the magnetic material from the liquid suction line by providing control of the electromagnet, even if its magnetism disappears or decreases to release the magnetic material from the liquid suction line together with the liquid. Needless to say, in order to form the electromagnet as described above, an exciting coil can be directly attached to the liquid suction line or the coil can be wound around the liquid suction line. The structure of moving the electromagnet closer to or away from the liquid suction line can also be used to achieve this purpose.

As an important feature of the present invention, the liquid suction line is formed by detachably mounting the pipette chip on the tip area of the liquid suction side itself. The magnet is arranged so that the magnetism generated by the magnet can have an effect on the magnetic material in the pipette chip.

Therefore, when the pipette chip is used to attract or release the magnetic material contained in the liquid, and the magnetic material contained in the liquid is attracted to the inner surface of the pipette chip, the magnetic material will be captured as completely as possible. It is also possible to transfer the pipette chip with magnetic material deposited on its inner surface to the next reaction or processing step. The above-mentioned technology cannot be realized without the suction pipe device according to the present invention, so the present invention is unprecedented in terms of this concept at least.

The above-mentioned pipette chip can only be reused for the same sample in one process, because the sample is processed according to a specific detection process in order to avoid cross-contamination. Any number of pipette chips can be used for the same sample according to the requirements of the reaction or processing procedure in each type of detection.

At the same time, in the present invention, if the liquid suction line is formed by a nozzle system and the pipette chip cannot be installed or removed, it can separate the magnetic material from the liquid by sucking or releasing the liquid , Stir and clean the liquid suction line inside and outside the liquid contact area in contact with the liquid to the extent that cross-contamination does not occur.

Another feature of the present invention includes the performance of separating, stirring and cleaning the magnetic material from the liquid by sucking the liquid into the liquid suction line or releasing the liquid from the liquid absorption line one or more times.

More specifically, in the present invention, the separation of the magnetic material from the liquid is performed by maintaining the magnetic material attracted and deposited on the inner surface of the liquid suction line to release only the liquid. This task is performed repeatedly and interactively as follows, namely by inserting a pipette chip with a magnetic material attracted by a magnet and deposited on its inner surface into a liquid stored in another container, and then repeatedly attracting and releasing this The liquid to this magnetic material is not affected by the magnetism of the above-mentioned magnet.

Therefore, by repeatedly sucking and releasing the liquid on the liquid suction line of the pipette device to perform the separation between the magnetic material contained in the liquid and the liquid, it is possible to almost completely capture the magnetic material. The complete separation of the magnetic material from the liquid containing the magnetic material can be achieved in all procedures that require the separation of the magnetic material from the liquid containing it.

At the same time, in the present invention, when the pipette chip is placed on the liquid suction line, the above-mentioned stirring and cleaning steps are performed by transferring the pipette with the magnetic material attracted by the magnet and deposited on its inner surface Move the chip to the position for stirring and cleaning, and then repeat the operation for sucking and releasing the liquid. In this example, when the magnetic material is deposited on the inner surface of the pipette chip, the operation of stirring or cleaning or sucking and releasing the liquid can be performed one or more times until the magnetic material is no longer affected by the magnet.

As described above, with the present invention, it is possible to simultaneously diffuse the magnetic material in the liquid by absorbing and releasing the liquid in the liquid suction line of the pipette chip. It may also improve its cleaning efficiency. In addition, although the suction and release of the liquid is performed between the liquid suction line and the container, the liquid containing the magnetic material will not splash out of the container. Therefore, the stirring and cleaning procedures can be performed under a stable condition and there is no possibility that the measurement accuracy will be low due to the splashing of the liquid containing the magnetic material.

It should be understood that in the present invention, the operation for separating the magnetic material from the liquid containing the magnetic material is to perform stirring and cleaning by transferring the magnet to the liquid containing the magnetic material, where the liquid was previously stored In a liquid storage area in a box with a plurality of liquid storage areas. The suction or release of liquid is performed according to its needs, or by maintaining the state in which the magnetic material is deposited on the inner surface of the pipette chip, releasing the remaining liquid from the container. Then, the liquid required for the next procedure is poured into the same container, and the pipette chip is used to suck and release the newly poured liquid. In short, in the present invention, when sucking and releasing the liquid on the liquid suction line, it is not necessary to use any special type of container, and the operation for separating, mixing and cleaning the magnetic material from the liquid containing the magnetic material can be performed. .

Another important feature of the present invention is that it is possible to perform both quantitative and qualitative identification of the target material contained in the liquid by precisely controlling the amount of liquid sucked by the liquid suction line.

The method according to the present invention can be applied and effective in a reaction between a magnetic material and a liquid that does not contain any magnetic material, a reaction between a magnetic material and a material existing in a liquid, and a magnetic material and other physically capable Or the reaction of the material chemically deposited on the magnet. This material includes immune materials, biological materials, and molecular biological materials such as antigens, antibodies, proteins, enzymes, DNA, vector DNAs, RNAs, or plasmids. This method can be applied to isotopes, enzymes, and other detection or analyzers for chemiluminescence, fluorine luminescence, and electroluminescence or similar labeling materials that need to be qualitatively or quantitatively analyzed. For example, the method according to the present invention can be applied to devices used in immunoassays, inspections using chemical reactions, extraction, recovery, and DNA separation.

For example, if the method of the present invention is applied to a chemical immunity inspection device, a container is formed in a cassette with a plurality of liquid storage areas. The liquid or reagent required for the reaction or treatment is poured into each liquid storage area, and the container is preferably able to be transferred together with the magnetic material attracted by the magnet and deposited on the inner surface of the liquid suction line. In this example, the liquid is poured into each liquid storage area as described above in advance, and only part of the liquid will be processed or gradually processed during the processing procedure.

Furthermore, for example, the sample can be quantitatively measured directly in the mother sample container, and then poured into each liquid storage area. It should be noted that the liquid storage area in the cassette can be arranged in a single row or in a plurality of rows, and be shaped like a microplate. If the shape of the cassette is like a microdisk, the multi-channel system can be realized by placing a plurality of liquid suction lines in a manner corresponding to the liquid storage area array, and therefore its processing capability can be significantly improved.

Other objects and characteristics of the present invention will be more apparent from the following description with reference to related drawings.

Schematic description