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Full description

Sister Ar'a's background

In some embodiments, the present invention relates to apparel and products with performance similar to silk, such as fabrics coated with proteins or protein fragments based on pure silk fibroin.

Silk is a polymer produced by a variety of insects and spiders

<p dir="rtl">5 A medullary protein, silk fibroin, and a gall-like coating composed of a non-fibrillar protein, sericin. Silk fibers are lightweight, permeable to gases, and slightly hypoallergenic. Silk is comfortable when worn against the skin and insulates it very well, keeping the wearer warm in cold temperatures and being cooler than many other fabrics in warm temperatures.</p>

U.S. Application No. 20040199241 relates to cement grafts containing silk, and methods for preparing...

<p dir="rtl">10 And the use of these stent baits. It is noteworthy that silk stimulates the in vivo adhesion of the stent graft to the vascular site, or induces or accelerates the fibrotic reaction in vivo, making the stent graft adhere to the vascular site.</p>

U.S. Application No. 20080188152 relates to a moisture vapor permeable and water resistant fiber having a base fabric layer, a moisture vapor permeable layer, and a water absorbent layer. Class is obtained

<p dir="rtl">15 Moisture vapor permeability by coating or laminating the inner side of the fabric with a moisture vapor permeable resin, which is said to prevent the penetration of water droplets but allow the penetration of moisture vapor molecules. The moisture absorbing layer consists of a mixture of moisture-wicking and/or inorganic powder, which is coated or drip-printed on the inner side of the moisture-, vapor- and water-resistant film.</p>

<p dir="rtl">20 General description of the invention</p>

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In this application, silk-like compression garments and methods for preparing them are disclosed. According to the images shown herein, the present invention relates to a product, including, but not limited to, clothing, linings, shoes, gloves, bags, furs, jewelry, and bags, adapted to be worn or carried on the body, which is processed At least partially superficial with an aqueous solution of pure silk fibroin-based protein fragments of the present invention such that a silk coating is produced on the product. In an embodiment, the product is manufactured from a nonwoven material. In an embodiment, desired additives can be added to the aqueous solution from pure silk fibroin-based protein fragments of the present invention such that a silk coating with the desired additives is produced.

In accordance with the images shown herein, an aqueous solution of pure silk fibroin 10 based protein fragments is available in a spray form that may be sprayed onto a product, including but not limited to,

Clothing, linings, shoes, gloves, bags, furs, jewelry, and bags, or to spray directly on the consumer’s body, to impart the desired properties to the product. In an embodiment, the product is manufactured from a woven material. In an embodiment, the product is manufactured from a nonwoven material. In one embodiment, the desired additives may be added to an aqueous solution of pure silk fibroin-based protein fragments of the invention.

<p dir="rtl">15 The current method produces a silky coating with the required additives.</p>

In one embodiment, textiles comprising a silk coating of the present invention are sold to a consumer. In an embodiment, the textiles of the present invention are used in the production of athletic apparel. In an embodiment, the textiles of the present invention are used in the production of fitness apparel. In an embodiment, the textiles of the present invention are used in the production of performance garments. In one embodiment, nonwovens are used

<p dir="rtl">20 The current invention in the production of golf clothing. In one embodiment, the patented textiles are used</p>

Current production of women's underwear. In an embodiment, the silk coating of the present invention is applied to the lining of an athletic garment. In an embodiment, the silk coating of the present invention is applied to the shell, lining, or interlining of an athletic apparel/apparel. In one embodiment, the sports apparel/apparel is made partly of silk-coated textiles of the present invention,

<p dir="rtl">25 It is partly made of uncoated textiles. In one embodiment, it manufactures sports apparel/apparel</p>

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Partly made of silk-coated textiles and partly made of uncoated textiles combining an uncoated inert synthetic material with a silk-coated inert synthetic material. Examples of inert synthetic material include, but are not limited to, polyester, polyamide, polyaramid, polytetrafluoroethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane and polyethylene glycol, ultra high molecular weight polyethylene , high-pressure polyethylene, nylon, LYCRA (polyester-polyurethane copolymer, also known as SPANDEX, and elastomer), and mixtures thereof. In an embodiment, sports apparel/apparel is made partly of silk-coated textiles and partly made of uncoated textiles comprising an elastomer material at least partly covered by a silk coating of the present invention. In an embodiment, the percentage of silk to elastomer can be varied to achieve the desired shrink or wrinkle resistant properties

and the desired moisture content versus the skin surface.

In an embodiment, the silk coating of the present invention is applied to an inner layer of a shoe (woven or non-woven based). In an embodiment, the silk coating of the present invention applied to an inner layer of a shoe helps maintain the most appropriate microenvironment for the foot, such as temperature And humidity while reducing any

<p dir="rtl">15 If rice increases sweat.</p>

In one embodiment, the silk coating of the present invention is visible. In an embodiment, the silk coating of the present invention is transparent. In an embodiment, the silk coating of the present invention applied to an athletic apparel/apparel helps control the transfer of a fluid away from the skin of a person wearing the apparel. In an embodiment, the silk coating of the present invention applied to sportswear/apparel has a texture

<p dir="rtl">20 Soft against the skin, reducing the friction of the fibers against the skin. In an embodiment, the silk coating of the present invention applied to textiles has properties that impart at least one of wrinkle resistance, shrinkage resistance, or machine washability to the textile. In an embodiment, the silk-coated textiles of the present invention are 100% machine washable and can be dry cleaned. In an embodiment, the silk-coated textiles of the present invention are 100% waterproof. in</p>

<p dir="rtl">25 In one embodiment, the silk-coated textiles of the present invention are shrink-resistant. In some form</p>

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Silk coated fabric improves skin health. In an embodiment, skin can be determined to be healthy by visual observation of uniform skin health. In an embodiment, skin can be determined to be healthy by visually observing a combined effect of smoothness and flushing. In one embodiment, the silk-coated fabric reduces skin irritation. In an embodiment, reducing skin irritation can reduce skin bumps and ulcerations. in

<p dir="rtl">5 In some cases, reducing skin irritation can result in a reduction in skin irritation or redness. In an embodiment, a reduction in skin irritation can produce a reduction in itching or burning. In an embodiment, the silk-coated fabric reduces skin inflammation. In an embodiment, the silk-coated textiles of the present invention have the characteristics of being water repellent, gas permeable, elastic, and having a number of other qualities highly desirable in sportswear. In an embodiment, the silk-coated textiles of the present invention</p>

<p dir="rtl">10 The silk fabric of the present invention also includes insulated fibers of the LYRCA (polyester-polyurethane copolymer) type.</p>

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In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a gas-permeable fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a waterproof fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is an anti-shrink fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a machine washable fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is an anti-wrinkle fabric. In an embodiment, textiles coated at least partially with an aqueous solution of pure silk fibroin-based protein fragments of the present invention provide moisture and vitamins to the skin.

In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have a cumulative unidirectional transparency coefficient &lt; 140. In an embodiment, textiles at least partially coated with an aqueous solution of protein fragments have

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The pure silk fibroin-based basis of the present invention has a unidirectional transparency coefficient T arc &lt; 120. In an embodiment, textiles at least partially coated with an aqueous solution of protein fragments based on pure silk fibroin of the present invention have a unidirectional transparency coefficient T arc &lt; 100. In an embodiment Thus, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have a cumulative unidirectional transparency coefficient &lt; 80.

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In an embodiment, textiles at least partially coated with an aqueous solution of fibroin-based protein fragments of the present invention have an overall humidity control capacity of &lt; 0.4. In an embodiment, textiles at least partially coated with an aqueous solution of fibroin-based protein fragments of the present invention have an overall humidity control capacity of &lt; 0.35. In an embodiment, textiles at least partially coated with an aqueous solution of fibroin-based protein fragments of the present invention have an overall humidity control capacity of &lt; 0.3. In an embodiment, textiles at least partially coated with an aqueous solution of fibroin-based protein fragments of the present invention have an overall humidity control capacity of &lt; 0.25.

In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have a wetting time of at least 3 seconds. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have a wetting time of at least 2.5 seconds. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have a wetting time of at least 2 seconds. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have a wetting time of at least 1.5 seconds.

In an embodiment, textiles at least partially coated with pure silk fibroin-based protein fragments of the present invention have a face absorption time of at least 50 seconds. In an embodiment, textiles at least partially coated with pure silk fibroin-based protein fragments of the present invention have a face absorption time of at least 40 seconds. In an embodiment, it is for partially coated textiles

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At least by protein fragments based on pure silk fibroin. The current invention has a face absorption time of at least 30 seconds.

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In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have an emergence absorption time of at least 80 seconds. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have an apparent absorption time of at least 70 seconds. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have an apparent absorption time of at least 60 seconds. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have an apparent absorption time of at least 50 seconds. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have an apparent absorption time of at least 40 seconds.

In an embodiment, the tissue at least partially coated by an aqueous solution of pure silk fibroin-based protein fragments of the present invention has a diffusion velocity of at least 1.6 mm/s. In an embodiment, the tissue at least partially coated by an aqueous solution of pure silk fibroin-based protein fragments of the present invention has a diffusion velocity of at least 1.4 mm/s. In an embodiment, the tissue at least partially coated by an aqueous solution of pure silk fibroin-based protein fragments of the present invention has a diffusion velocity of at least 1.2 mm/s. In an embodiment, the tissue at least partially coated by an aqueous solution of pure silk fibroin-based protein fragments of the present invention has a diffusion velocity of at least 1.0 mm/s. In an embodiment, tissues at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention have a diffusion velocity of at least 0.8 mm/s.

In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit microbial growth of less than 2000% over a 24-hour period.

25 In an embodiment, the textiles appear to be at least partially coated by an aqueous solution of protein fragments

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Based on pure silk fibroin, the current invention has less than 1000% microbial growth over 24 hours. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 500% microbial growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein 5 fragments of the present invention exhibit less than 400% microbial growth over a 24-hour period.

In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 300% microbial growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 200% microbial growth over a 24-hour period.

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In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 2,000% bacterial growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 1,000% bacterial growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 500% bacterial growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 400% bacterial growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 300% bacterial growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 200% bacterial growth over a 24-hour period.

In an embodiment, the textiles appear to be at least partially coated by an aqueous solution of protein fragments

Based on pure silk fibroin, the current invention has less than 2000% fungal growth over 24 hours.

In an embodiment, the textiles appear to be at least partially coated by an aqueous solution of protein fragments

25 Based on pure silk fibroin, the current invention has less than 1000% fungal growth over 24 hours.

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In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 500% fungal growth over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit fungal growth of less than 400% over a 24-hour period. in

<p dir="rtl">5 In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit fungal growth of less than 300% over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 200% fungal growth over a 24-hour period.</p>

In an embodiment, the textiles appear to be at least partially coated by an aqueous solution of protein fragments

<p dir="rtl">10 Based on pure silk fibroin, the current invention has a growth rate of Staphylococcus aureus of less than 2000% over 24 hours. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit less than 1,000% Staphylococcus aureus growth over a 24-hour period. In an embodiment, textiles coated at least partially with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit growth.</p>

<p dir="rtl">15 Stataphylococcus aure less than 500% over 24 hours. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit Staphylococcus aureus growth of less than 400% over a 24-hour period. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit Staphylococcus aureus growth of less than</p>

<p dir="rtl">20 300% over 24 hours. In an embodiment, at least partially coated textiles are shown by</p>

An aqueous solution of pure silk fibroin-based protein fragments of the present invention results in growth of Staphylococcus aureus of less than 200% over 24 hours.

In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit Klebsiella pneumoniae growth of less than 2000%.

25 Over 24 hours. In an embodiment, textiles are shown to be at least partially coated with a solution

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Hydrolyzed protein fragments based on pure silk fibroin for the current invention. Growth of Klebsiella pneumoniae is less than 1000% over 24 hours. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit Klebsiella pneumoniae growth of less than 500% over a 24-hour period. In 5 one embodiment, the textiles are shown to be at least partially coated by an aqueous solution of protein fragments

Based on pure silk fibroin, the current invention has a growth rate of Klebsiella pneumoniae of less than 400% over 24 hours. In an embodiment, textiles at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention exhibit Klebsiella pneumoniae growth of less than 300% over a 24-hour period. In an embodiment, the textiles 10 are at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the invention.

Current growth of Klebsiella pneumoniae is less than 200% over 24 hours.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present invention is used for a textile coating. In one embodiment, the concentration of silk in the solution varies between about 0.1 and 20.0%. In one embodiment, the concentration of silk in the solution varies between about 0.1 and 15.0%. In 15 samples, the concentration of silk in the solution ranges between about 0.5 and 10.0%. In some form, I go

The concentration of silk in the solution is between about 1.0 and 5.0%. In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present invention is applied directly to a fabric. Alternatively, a fine iron ball and any fabric coating additives can be used. In an embodiment, additives can be added to an aqueous solution of pure silk fibroin-based protein fragments of the present invention prior to coating (such as 20 alcohols) to further enhance the properties of the material. In an embodiment, the silk coating of the present invention

It can have a pattern to make the properties of silk on the fabric the most suitable. In an embodiment, the coating is applied to the fabric under tension and/or ductility in order to vary the penetration of the fabric.

In an embodiment, the silk coating of the present invention may be applied to a yarn plane, and then fabric can be produced once the yarn is coated. In an embodiment, an aqueous solution may be spun from fibroin-based protein fragments

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Pure silk is currently invented into fibers to make silk fabric and/or blend silk fabric with other materials known in the clothing industry.

In an embodiment, a method for coating fabric with silk includes immersing the fabric in any aqueous solution of pure silk fibroin-based protein fragments of the present invention. In an embodiment, the method of coating fabric with silk

<p dir="rtl">5 Spraying included. In one embodiment, the method of coating silk fabric includes chemical vapor deposition. In one embodiment, the method of coating silk fabric includes electrochemical coating. In one embodiment, the method of coating a silk fabric includes coating with a knife to spread any aqueous solutions of pure silk fibroin-based protein fragments of the present invention onto the fabric. The coated fabric can then be air-dried, dried under a heat/air flow, or cross-linked to the fabric surface. In an embodiment, it includes a process</p>

<p dir="rtl">10 Drying treatment with additives and/or atmospheric conditions.</p>

In accordance with the images shown herein, methods for preparing aqueous solutions of protein fragments based on pure silk fibroin are disclosed. In an embodiment, a solution is produced from a mixture of at least one pure silk fibroin (SPF) based protein fragments having a medium molecular weight (MW) range with respect to a medium specific gravity and polydispersity. In an embodiment, a solution of at least one SPF mixture is produced therefor

<p dir="rtl">15 The MW range is between about 6 and 16 kDa, and the polydispersity range is between about 1.5 and 3.0. In an embodiment, a solution is produced from at least the SPF mixture having an MW range between about 17 and 38 kDa, and a polydispersity range between about 1.5 and 3.0. In an embodiment, a solution is produced from at least the SPF mixture having an MW range between about 39 and 80 kDa, and a polydispersity range between about 1.5 and 3.0.</p>

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In accordance with the images shown herein, a composition has been disclosed containing protein fragments based on pure silk fibroin that are primarily devoid of sericin, wherein the composition has an average molecular weight ranging between about 6 and 16 kDa, and wherein the composition has a polydispersity between about 1.5 and 3.0, wherein the composition is essentially homogeneous and wherein the composition includes between zero and about 500 ppm inorganic residues and between zero and about 500 ppm organic residues. In one embodiment, the protein fragments are based on silk fibroin

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Pure between about 10 and 300 ppm lithium bromine residues and between about 10 and 100 ppm sodium carbonate residues. In an embodiment, the lithium bromide residues can be measured using a high-pressure liquid chromatography lithium bromide experiment, and the sodium carbonate residues can be measured using the sodium carbonate experiment by

<p dir="rtl">5 High pressure liquid chromatogruff. In one embodiment, the composition also includes less than 10% water. in</p>

In one embodiment, the composition is in the form of a solution. In one embodiment, the composition includes between about 0.1 and 30.0 by weight pure silk fibroin-based protein fragments. The protein fragments based on pure silk fibroin are stable in solution for at least 30 days. In an embodiment, the term “steady” refers to the absence of spontaneous or gradual gel formation, with no visible change in color or turbidity

<p dir="rtl">10 Solution. In an embodiment, the term “stable” refers to the fragments not clumping and the molecular weight not increasing with time, and in an embodiment, the composition is in the form of an aqueous solution. In an embodiment, the composition is in the form of an organic solution. The composition may be supplied in a closed container. In some embodiments, the composition also includes one or more molecules selected from a group consisting of therapeutic agents, growth factors, antioxidants, proteins, vitamins, carbohydrates, polyamides, and acids.</p>

<p dir="rtl">15 Nucleic acids, salts, acids, bases, biomolecules, glycosamino glycans, polysaccharides, extracellular template molecules, metal ion, metal oxide, synthetic molecules, polyanhydrides, cells, fatty acids, perfume, minerals, plants, extracts. Vegetables, preservatives, and essential oils. In an embodiment, the added molecules or molecules are stable (i.e., retain their activity over time) within the formulation and can be released at a desired rate. In an embodiment, a single molecule</p>

<p dir="rtl">20 One or more of them is vitamin C or a derivative thereof. In one embodiment, the composition also includes an alpha hydroxy acid selected from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. In an embodiment, the composition also includes hyaluronic acid or its salts in a concentration of about 0.5 to 10.0%. In an embodiment, the composition also includes at least one zinc oxide or titanium oxide. In one embodiment, the ferroin-based protein fragments are pure silk in</p>

<p dir="rtl">25 The composition has a slight allergic effect. In an embodiment, the protein fragments based on pure silk fibroin are biocompatible, non-allergenic, and non-immunogenic.</p>

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In accordance with the images shown herein, a composition has been disclosed containing protein fragments based on pure silk fibroin that are primarily devoid of sericin, wherein the composition has an average molecular weight of between about 17 and 38 kDa, and wherein the composition has a polydispersity between about 1.5 and 3.0, wherein the composition is mainly homogeneous and wherein the composition includes between

<p dir="rtl">5 Zero and approximately 500 ppm inorganic residues and between zero and approximately 500 ppm organic residues. In one embodiment, the protein fragments based on pure silk fibroin have between about 10 and 300 ppm lithium bromine residues and between about 10 and 100 ppm sodium carbonate residues. In an embodiment, the lithium bromide residues can be measured using a high-pressure liquid chromatography lithium bromide assay,</p>

<p dir="rtl">10 Sodium carbonate building blocks can be measured using a sodium carbonate measurement experiment using high-pressure liquid chromatography. In one embodiment, the composition also includes less than 10% water. In one embodiment, the composition is in the form of a solution. In one embodiment, the composition includes between about 0.1 and 30.0 by weight pure silk fibroin-based protein fragments. The protein fragments based on pure silk fibroin are stable in solution for at least 30 days. In one embodiment, the term refers to:</p>

<p dir="rtl">15 “Constant” means no spontaneous or gradual gel formation, with no visible change in the color or turbidity of the solution. In an embodiment, the term “stable” refers to the fragments not clumping and the molecular weight not increasing with time, and in an embodiment, the composition is in the form of an aqueous solution. In one embodiment, the composition is in the form of an organic solution. The composition may be supplied in a closed container. In some embodiments, the composition also includes one or more molecules selected from a group consisting of agents</p>

<p dir="rtl">20 Therapeutic, growth factors, antioxidants, proteins, vitamins, carbohydrates, polymers, nucleic acids, salts, acids, bases, biomolecules, glycosamino glycans, polysaccharides, extracellular matrix molecules, metal ions, metal oxides , synthetic molecules, polyanhydrides, cells, fatty acids, fragrance, minerals, plants, plant extracts, preservatives, and essential oils. In an embodiment, the added molecules or molecules are stable (i.e., they retain their activity).</p>

<p dir="rtl">25 over time) within the composition and can be released at a desired rate. In an embodiment, the one or more molecules are vitamin C or a derivative thereof. In an embodiment, the composition also includes alpha acid</p>

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Hydroxy selected from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. In an embodiment, the composition also includes hyaluronic acid or its salts in a concentration of about 0.5 to 10.0%. In an embodiment, the composition also includes at least one zinc oxide or titanium oxide. In an embodiment, the pure silk ferroin-based protein fragments in the composition have a minimal allergenic effect. In an embodiment, the protein fragments based on pure silk fibroin are biocompatible, non-allergenic, and non-immunogenic.

In accordance with the images shown herein, a composition has been disclosed containing protein fragments based on pure silk fibroin that are mainly devoid of sericin, wherein the composition has an average molecular weight ranging between approximately 39 and 80 kDa, and wherein the composition has polydispersity between

<p dir="rtl">10 About 1.5 and 3.0, wherein the composition is mainly homogeneous and wherein the composition includes between 0 and about 500 ppm inorganic residues and between 0 and about 500 ppm organic residues. In one embodiment, the protein fragments based on pure silk fibroin have between about 10 and 300 ppm lithium bromine residues and between about 10 and 100 ppm sodium carbonate residues. In a model, building blocks can be measured</p>

<p dir="rtl">15 Lithium bromide using a high-pressure liquid chromatography experiment, and sodium carbonate residues can be measured using a high-pressure liquid chromatography experiment. In one embodiment, the composition also includes less than 10% water. In one embodiment, the composition is in the form of a solution. In one embodiment, the composition includes between about 0.1 and 30.0 by weight pure silk fibroin-based protein fragments. Protein fragments are their basis</p>

<p dir="rtl">20 Pure silk fibroin is stable in solution for at least 30 days. In an embodiment, the term “steady” refers to the absence of spontaneous or gradual gel formation, with no visible change in the color or turbidity of the solution. In an embodiment, the term “stable” refers to the fragments not clumping and the molecular weight not increasing with time, and in an embodiment, the composition is in the form of an aqueous solution. In one embodiment, the composition is in the form of an organic solution. The composition may be supplied in a closed container. In some</p>

<p dir="rtl">25 In embodiments, the composition also includes one or more molecules selected from a group consisting of agents</p>

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Therapeutic, growth factors, antioxidants, proteins, vitamins, carbohydrates, polymers, nucleic acids, salts, acids, bases, biomolecules, glycosamino glycans, polysaccharides, extracellular matrix molecules, metal ions, metal oxides Synthetic molecules, polyanhydrides, cells, fatty acids, perfume, minerals, plants, plant extracts, preservatives, and oils.

<p dir="rtl">5 Basic. In an embodiment, the added molecules or molecules are stable (that is, they retain their activity over time) within the composition and can be released at a desired rate. In an embodiment, the one or more molecules are vitamin C or a derivative thereof. In an embodiment, the composition also includes an acid alpha</p>

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Hydroxy selected from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. In an embodiment, the composition also includes hyaluronic acid or its salts in a concentration of about 0.5 to 10.0%. In an embodiment, the composition also includes at least one zinc oxide or titanium oxide. In an embodiment, the pure silk ferroin-based protein fragments in the composition have a minimal allergenic effect. In an embodiment, the protein fragments based on pure silk fibroin are biocompatible, non-allergenic, and non-immunogenic.

According to the images shown herein, a gel containing protein based fragments has been detected

<p dir="rtl">15 Pure silk fibroin is mainly devoid of sericin and contains: an average molecular weight of approximately 17 to 38 kDa; It is polydispersed between about 1.5 and 3.0, water between about 20 and 99.9% by weight, wherein the gel contains between 0 and 500 ppm inorganic residues, and wherein the gel contains between 0 and 500 ppm organic residues. In one embodiment, the gel includes between 1.0 and 50.0% crystalline protein domains. In the form include</p>

<p dir="rtl">20 All contain between about 0.1 and 6.0% by weight protein fragments based on pure silk fibroin. In an embodiment, the gel has a pH between about 1.0 and 7.0. In one embodiment, the gel also includes between about 0.5 and 20.0% by weight vitamin C or a derivative thereof. In one embodiment, vitamin C or a derivative thereof remains stable within the gel for a period between approximately 5 days and 5 years. In one embodiment, vitamin C or a derivative thereof is immobilized within the gel resulting in its biologically active release. In one embodiment, the gel also includes an addition</p>

<p dir="rtl">25 Selected from a group consisting of vitamin E, frankincense oil, rose oil, lemon juice, and oil</p>

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Lemongrass and caffeine. In one embodiment, the gel is packed in an airtight container. In an embodiment, the protein fragments based on pure silk fibroin have little effect to cause sensitization. In one embodiment, the gel has less than 10 CFU/mL.

According to the images shown in this application, a method for preparing an aqueous solution of

<p dir="rtl">5 Protein fragments based on pure silk fibroin have an average molecular weight ranging between about 6 and 16 kilodaltons. The method includes the following steps: removing the gum of the silk source by adding the silk source to a boiling aqueous solution (100°C) of sodium carbonate for a treatment period of about 30 to 60 minutes. minutes, removing sericin from the solution to produce a silk fibroin extract containing undetectable levels of sericin, filtering the solution from the silk fibroin extract, and dissolving the silk fibroin extract</p>

<p dir="rtl">10 In a lithium bromide solution, the starting temperature when placing the silk fibroin extract in the lithium bromide solution ranges between about 60 and 140 °C, and keeping the lithium bromide-silk fibroin solution in an oven with a temperature of about 140 °C for a period of at least 1 hour, and removing Lithium bromide from silk fibroin extract, and producing an aqueous solution from silk protein fragments. The aqueous solution includes: fragments with an average molecular weight ranging between about 6 and 16 kilodaltons; And where the solution is</p>

<p dir="rtl">15 Hydrolyzed protein fragments based on pure silk fibroin are polydisperse, ranging between about 1.5 and 3.0. In an embodiment, the method includes a step of drying the silk fibroin extract prior to a dissolution step. In an embodiment, the amount of lithium bromide residues in an aqueous solution can be measured using a high-pressure liquid chromatography-based lithium bromide measurement experiment. In an embodiment, the amount of sodium carbonate residues in the aqueous solution can be measured using a sodium carbonate measurement experiment</p>

<p dir="rtl">20 Using high-pressure liquid chromatography. In one embodiment, the method includes the step of adding a therapeutic agent to an aqueous solution of purified silk fibroin-based protein fragments. In one embodiment, the method includes the step of adding a selected molecule of one antioxidant or enzyme to an aqueous solution of purified silk fibroin-based protein fragments. In one embodiment, the method includes the step of adding a vitamin to an aqueous solution of pure silk fibroin-based protein fragments. In a model, a choice is made</p>

<p dir="rtl">25 The vitamin is derived from vitamin C or derived from it. In an embodiment, the method also includes an addition step</p>

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Alpha hydroxy acid to the aqueous solution of pure silk fibroin-based protein fragments. In one embodiment, the alpha hydroxy acid is selected from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. In an embodiment, the method further includes the step of adding hyaluronic acid at a concentration between about 0.5 and 10.0% to the aqueous solution of the protein fragments.

<p dir="rtl">5 It is based on fibroin. In an embodiment, the method further comprises adding at least one zinc oxide or titanium dioxide to the aqueous solution of fibroin-based protein fragments.</p>

According to the images shown in this application, a method has been disclosed for preparing an aqueous solution of protein fragments based on pure silk fibroin with an average molecular weight that ranges between approximately 17 and 38 kilodaltons. The method includes the following steps: removing the silk source gum by adding a source

<p dir="rtl">10 Silk into a boiling aqueous solution (100°C) of sodium carbonate for a treatment period of about 30 to 60 minutes, removing the sericin from the solution to produce a silk fibroin extract containing undetectable levels of sericin, filtering the solution from the silk fibroin extract, and dissolving the silk fibroin extract in a bromide solution. Lithium has a starting temperature when placing silk fibroin extract in a lithium bromide solution, which ranges between about 80 and 140 degrees Celsius, and the solution is preserved from lithium bromide-silk fibroin.</p>

<p dir="rtl">15 In an oven with a temperature of about 140°C for a period of at least 1 hour, remove the lithium bromide from the silk fibroin extract, and produce an aqueous solution of silk protein fragments, wherein the aqueous solution of protein fragments based on pure silk fibroin contains lithium bromide residues between about 10 and 300 ppm, wherein the aqueous solution comprises protein fragments based on pure silk fibroin containing sodium carbonate residues between about 10 and 100 ppm, and wherein the solution includes</p>

<p dir="rtl">20 The aqueous solution of protein fragments based on pure silk fibroin contains fragments with an average molecular weight that varies between 17 and 38 kDa, and where the aqueous solution of protein fragments based on pure silk fibroin has a polydispersity between about 1.5 and 3.0. In an embodiment, the method includes a step of drying the silk fibroin extract prior to a dissolution step. In one embodiment, the amount of lithium bromide residues in an aqueous solution can be measured using a high-Arf liquid chromatography lithium bromide measurement experiment.</p>

<p dir="rtl">25 the pressure. In an embodiment, the amount of sodium carbonate residues in the aqueous solution can be measured</p>

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Using an experiment to measure sodium carbonate using high-pressure liquid chromatography. In one embodiment, the method includes the step of adding a therapeutic agent to an aqueous solution of purified silk fibroin-based protein fragments. In one embodiment, the method includes the step of adding a selected molecule of one antioxidant or enzyme to an aqueous solution of purified silk fibroin-based protein fragments. in

<p dir="rtl">5 In one embodiment, the method includes the step of adding a vitamin to an aqueous solution of pure silk fibroin-based protein fragments. In one embodiment, the vitamin is selected from or derived from vitamin C. In an embodiment, the method further includes the step of adding an alpha hydroxy acid to an aqueous solution of purified silk fibroin-based protein fragments. In one embodiment, the alpha hydroxy acid is selected from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. In an example,</p>

<p dir="rtl">10 The method also includes the step of adding hyaluronic acid at a concentration between about 0.5 and 10.0% to the aqueous solution of fibroin-based protein fragments. In an embodiment, the method further comprises adding at least one zinc oxide or titanium dioxide to the aqueous solution of fibroin-based protein fragments.</p>

According to the images shown in this application, a method for preparing an aqueous solution of

<p dir="rtl">15 Protein fragments based on pure silk fibroin have an average molecular weight ranging between about 39 and 80 kilodaltons. The method includes the following steps: removing the gum of the silk source by adding the silk source to a boiling aqueous solution (100°C) of sodium carbonate for a treatment period of about 30 to 60 minutes. minute, removing sericin from the solution to produce a silk fibroin extract containing undetectable levels of sericin, filtering the solution from the silk fibroin extract, and dissolving the silk fibroin extract</p>

<p dir="rtl">20 In a lithium bromide solution, the starting temperature when placing the silk fibroin extract in the lithium bromide solution ranges between about 80 and 140 °C, and keeping the lithium bromide-silk fibroin solution in an oven with a temperature of about 140 °C for a period of at least 1 hour, and removing Lithium bromide from an extract of silk fibroin, and the production of an aqueous solution from silk protein fragments, wherein the aqueous solution from pure silk fibroin-based protein fragments comprises lithium bromide residues between about 10 and 300</p>

<p dir="rtl">25 ppm, wherein the aqueous solution comprises protein fragments based on pure silk fibroin</p>

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Sodium carbonate building blocks between approximately 10 and 100 ppm, wherein the aqueous solution of protein fragments based on pure silk fibroin comprises fragments with an average molecular weight of between 39 and 80 kDa, and wherein the aqueous solution of protein fragments based on pure silk fibroin has a dispersion Multiple between about 1.5 and 3.0. In an embodiment, the method includes a drying step

<p dir="rtl">5 Silk fibroin extract before dissolution step. In an embodiment, the amount of lithium bromide residues in an aqueous solution can be measured using a high-pressure liquid chromatography-based lithium bromide measurement experiment. In an embodiment, the amount of sodium carbonate residues in an aqueous solution can be measured using a high-pressure sodium carbonate measurement experiment with a high-pressure liquid chromatograph. In an embodiment, the method includes the step of adding a therapeutic agent to an aqueous solution from protein based fragments</p>

<p dir="rtl">10 Pure silk fibroin. In one embodiment, the method includes the step of adding a selected molecule of one antioxidant or enzyme to an aqueous solution of purified silk fibroin-based protein fragments. In one embodiment, the method includes the step of adding a vitamin to an aqueous solution of pure silk fibroin-based protein fragments. In one embodiment, the vitamin is selected from or derived from vitamin C. In an embodiment, the method further includes the step of adding an alpha hydroxy acid to the aqueous solution of the fragments</p>

<p dir="rtl">15 Protein based pure silk fibroin. In one embodiment, the alpha hydroxy acid is selected from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. In an embodiment, the method further includes the step of adding hyaluronic acid at a concentration between about 0.5 and 10.0% to the aqueous solution of fibroin-based protein fragments. In an embodiment, the method further comprises adding at least one zinc oxide or titanium dioxide to the aqueous solution of fragments</p>

<p dir="rtl">20 Fibroin-based protein.</p>

In accordance with the images shown herein, a method for producing silk gel with entrapped therapeutic molecules or agents such as those described in the following paragraphs is disclosed. In an embodiment, the at least one molecule or therapeutic agent of interest is physically trapped in a solution of the SPF mixture of the present invention while it is processed into aqueous gels. The hydro-silk gel of the present invention may be used to release

<p dir="rtl">25 At least one relevant molecule or therapeutic agent.</p>

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According to the images shown herein, pure silk fibroin-based protein fragments can be formed from aqueous solutions of the present invention into yarns and fabrics including, for example, woven fabrics, and such fabrics can be used in textiles as previously mentioned.

According to the images shown in this application, the silk fabric is manufactured from SPF mixture solutions

<p dir="rtl">5 The current sister has been revealed. In an embodiment, the molecule or therapeutic agent of interest is entrapped</p>

At least one is physically present in a solution of the SPF mixture of the present invention. A silk membrane of the present invention may be used to release at least one molecule or therapeutic agent of interest.

Brief explanation of the drawings

The currently disclosed models will be further explained by reference to the attached drawings. Graphics

<p dir="rtl">10 The explanations are not necessarily to scale, but instead focus on clarifying the principles of models currently disclosed in general terms.</p>

Figure (1): A process flow chart illustrating different embodiments for producing aqueous solutions from pure silk fibroin-based protein fragments (SPFs) of the present invention.

Figure (2): A process flow chart showing different parameters that can be modified during the SPFs production process

<p dir="rtl">15 To determine the current rate during the extraction and dissolution steps.</p>

Figure (3): A photograph showing dried silk fibroin extract.

Figure (4): An artistic photograph showing an example of SPF in the form of a solution of the present invention.

Figures (5A-5D): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at room temperature and melted in a 60°C oven for 4 hours (the temperature and extraction time for 20 sericin were different).

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Figures (6a-6d): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at room temperature and melted in an oven at 60°C for 6 hours (the temperature and time of sericin extraction were different).

Figures (7a-7d): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at

<p dir="rtl">5 Melted at room temperature in a 60°C oven for 8 hours (the temperature and time of sericin extraction were different).</p>

Figures (8a-8d): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at room temperature and melted in an oven at 60°C for 12 hours (the temperature and time of sericin extraction were different).

<p dir="rtl">10 Figures (9a-9d): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at room temperature and melted in an oven at 60°C for 24 hours (the temperature and time of sericin extraction were different).</p>

Figures (10a-10d): Royal photographs showing silk dissolved in lithium bromide (LiBr) solutions at room temperature and melted in a 60°C oven for 168/192 hours (the temperature and time were

<p dir="rtl">15 Different sericin extraction.</p>

Figures (11a-11c): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at room temperature and melted in an oven at 60°C for 1, 4, and 6 hours, where sericin extraction was performed at 100°C for 60 minutes.

Figures (12a-12d): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at

<p dir="rtl">20 Melted at room temperature in a 60°C oven for 1 hour (the temperature and time of sericin extraction were different).</p>

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Figures (13a-13d): Photographs showing silk dissolved in lithium bromide (LiBr) solutions at room temperature and melted in an oven at 60°C for 4 hours (the temperature and time of sericin extraction were different).

Figures (14a-14d): Royal photographs showing silk dissolved in lithium bromide (LiBr) solutions at 5°C room temperature, melted in an oven at 60°C for 6 hours (the temperature and extraction time were...

Different sericin.

Figures (15a-15d): Photographs showing silk dissolved in LiBr solutions at 80°C and melted in a 60°C oven for 1 hour (the temperature and time of sericin extraction were different).

Figures (16a-16d): Photographs showing silk dissolved in LiBr solutions at 80°C and melted in a 60°C oven for 4 hours (the temperature and time of sericin extraction were different).

Figures (17a-17d): Photographs showing silk dissolved in LiBr solutions at 80°C and melted in a 60°C oven for 4 hours (the temperature and time of sericin extraction were different).

Figures (18a-18d): Photographs showing silk dissolved in LiBr solutions at 100°C and melted in a 60°C oven for 4 hours (the temperature and time of sericin extraction were different).

<p dir="rtl">15 Figures (19a-19d): Photographs showing silk dissolved in LiBr solutions at 100°C and melted in a 60°C oven for 4 hours (the temperature and time of sericin extraction were different).</p>

Figures (20a-20d): Photographs showing silk dissolved in LiBr solutions at 100°C and melted in a 60°C oven for 6 hours (the temperature and time of sericin extraction were different).

Figures (21a-21d): Photographs showing silk dissolved in LiBr solutions at 140°C (point

<p dir="rtl">20 Boiling the melted LiBr in an oven at 60°C for 1 hour (the temperature and time of sericin extraction were different).</p>

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Figures (22a-22d): Photographs showing silk dissolved in LiBr solutions at 140°C (boiling point of LiBr) and melted in an oven at 60°C for 4 hours (the temperature and time of sericin extraction were different).

Figures (23a-23d): Royal photographs showing silk dissolved in LiBr solutions at 140°C (LiBr boiling point 5) and melted in an oven at 60°C for 6 hours (the temperature and time of sericin extraction were different).

Figures (24a-24d): Photographs showing silk dissolved in LiBr solutions at 80°C and melted in an oven at 80°C for 1 hour (the temperature and time of sericin extraction were different).

Figures (25a-25d): Photographs showing silk dissolved in LiBr solutions at 80°C and melted in an oven at 80°C for 4 hours (the temperature and time of sericin extraction were different).

Figures (26a-26d): Photographs showing silk dissolved in LiBr solutions at 80°C and melted in an oven at 80°C for 6 hours (the temperature and time of sericin extraction were different).

Figures (27a-27d): Photographs showing silk dissolved in LiBr solutions at 100°C and melted in a 100°C oven for 1 hour (the temperature and time of sericin extraction were different).

<p dir="rtl">15 Figures (28a-28d): Photographs showing silk dissolved in LiBr solutions at 100°C and melted in a 100°C oven for 4 hours (the temperature and time of sericin extraction were different).</p>

Figures (29a-29d): Photographs showing silk dissolved in LiBr solutions at 100°C and melted in a 100°C oven for 6 hours (the temperature and time of sericin extraction were different).

Figures (30a-30d): Photographs showing silk dissolved in LiBr solutions at 140°C (point

<p dir="rtl">20 Boiling the melted LiBr in an oven at 120°C for 1 hour (the temperature and time of sericin extraction were different).</p>

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Figures (31a-31d): Photographs showing silk dissolved in LiBr solutions at 140°C (boiling point of LiBr) and melted in an oven at 120°C for 4 hours (the temperature and time of sericin extraction were different).

Figures (32a-32d): Photographs showing silk dissolved in LiBr solutions at 140°C (point

5 Boiling LiBr) melted in an oven at 120°C for 6 hours (the temperature and time of sericin extraction were different).

Figure (33): shows HPLC chromatograms for samples containing vitamin C. Figure (33) shows peaks from (1) a sample of vitamin C chemically stabilized at atmospheric conditions and (2) a sample of vitamin C taken after 1 hour at atmospheric conditions without chemical stabilization To prevent oxidation, where the 10 analysis products are visible.

Figure (34): A table summarizing the concentrations of LIBr and sodium carbonate (Na2CO3) in silk protein solutions of the present invention.

Figure (35): Table summarizing the concentration of LiBr and Na2CO3 in silk protein solutions of the present invention.

Figure (36): Table summarizing the stability of vitamin C in chemically stabilized solutions.

<p dir="rtl">15 Figure (37): A table summarizing the molecular weights of silk protein solutions of the present invention.</p>

Figures (38a, 38b): Graphs representing the effect of extraction volume on % mass loss.

Figure (39): A table summarizing the molecular weights of dissolved silk from different LiBr aeration concentrations and from different extraction and dissolution volumes.

Figure (40): A graph summarizing the effect of extraction weight on the molecular weight of under-treated silk

<p dir="rtl">20 Extraction temperature conditions at 100°C, LiBr at 100°C, and melting oven at 100°C (oven/melting times were different).</p>

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Figure (41): A graph summarizing the effect of extraction weight on the molecular weight of silk processed under conditions of extraction temperature at 100°C, LiBr boiling, and melting oven at 60°C (oven/melting times were different).

Figure (42): A graph summarizing the effect of extraction weight on the molecular weight of under-treated silk

<p dir="rtl">5 Extraction temperature conditions at 100°C, LiBr at 60°C, and melting oven at 60°C (oven/melting times were different).</p>

Figure (43): A graph summarizing the effect of extraction weight on the molecular weight of silk processed under the conditions of extraction temperature at 100°C, LiBr at 80°C, and melting oven at 80°C (oven/melting times were different).

<p dir="rtl">10 Figure (44): A graph summarizing the effect of extraction weight on the molecular weight of silk processed under the conditions of extraction temperature at 100°C, LiBr at 80°C, and melting oven at 60°C (oven/melting times were different).</p>

Figure (45): A graph summarizing the effect of extraction weight on the molecular weight of silk processed under the conditions of extraction temperature at 100°C, LiBr at 100°C, and melting oven at 60°C (15 oven/melting times were different).

Figure (46): A graph summarizing the effect of extraction weight on the molecular weight of silk processed under the conditions of extraction temperature at 100°C, LiBr at 140°C, and melting oven at 140°C (the oven/melting times were different).

Figure (47): A graph summarizing the effect of extraction temperature on the molecular weight of processed silk

<p dir="rtl">20 Under conditions of 60 min extraction time, LiBr at 100°C, and melting oven at 100°C (oven/melting times were different).</p>

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Figure (48): A graph summarizing the effect of LiBr temperature on the molecular weight of silk processed under the conditions of an extraction time of 60 minutes, an extraction temperature of 100°C, and a melting oven at 60°C (the oven/melting times were different).

Figure (49): A graph summarizing the effect of LiBr temperature on the molecular weight of silk treated under

<p dir="rtl">5 Conditions of extraction time of 30 minutes, extraction temperature of 100°C, and melting oven at 60°C (oven/melting times were different).</p>

Figure (50): A graph summarizing the effect of oven/melting temperature on the molecular weight of silk processed under the conditions of extraction temperature at 100°C, 30-minute extraction weight, and lithium bromide at 100°C (oven/melting times were different).

<p dir="rtl">10 Figure (51): A graph summarizing the effect of oven/melting temperature on the molecular weight of silk processed under conditions of extraction temperature at 100°C, 60-minute extraction weight, and lithium bromide at 100°C (oven/melting times were different).</p>

Figure (52): A graph summarizing the effect of oven/melting temperature on the molecular weight of silk processed under the conditions of extraction temperature at 100°C, extraction weight of 30 minutes, and lithium bromide at

<p dir="rtl">15 140°C (oven/thawing times were different).</p>

Figure (53): A graph summarizing the effect of oven/melting temperature on the molecular weight of silk processed under the conditions of extraction temperature at 100°C, 30-minute extraction weight, and lithium bromide at 140°C (oven/melting times were different).

Figure (54): A graph summarizing the effect of oven/melting temperature on the molecular weight of processed silk

<p dir="rtl">20 Under conditions of extraction temperature at 100°C, extraction weighing of 60 minutes, and lithium bromide at 80°C (oven/melting times were different).</p>

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Figure (55): A graph summarizing the molecular weights of silk processed under different conditions, including extraction time, extraction temperature, lithium bromide (LiBr) temperature, melting oven temperature, and melting time in the oven.

Figure (56): A graph summarizing the molecular weights of silk processed under equal conditions

<p dir="rtl">5 Oven/melting temperature LiBr temperature.</p>

Figure (57a): A graph showing the wetting time using spray paint.

Figure (57b): A graph showing the wetting time using stencil coating.

Figure (57c): A graph showing the time of wetting the paint using a bath.

Figure (57d): A graph showing the time of wetting the paint using a sieve.

<p dir="rtl">10 Figure (58a): A graph showing the absorption time by spray paint.</p>

Figure (58b): A graph showing the absorption time by stencil coating.

Figure (58c): A graph showing the absorption time by paint using a bath.

Figure (58d): A graph showing the absorption time of paint using a sieve.

Figure (59a): A graph showing the speed of spread by spray paint.

<p dir="rtl">15 Figure (59b): A graph showing the speed of diffusion using stencil paint.</p>

Figure (59c): A graph showing the speed of spreading by paint using a bath.

Figure (59d): A graph showing the speed of spread of paint using a sieve.

Figure (60a): A graph showing the cumulative unidirectional transfer coefficient by spray coating.

Figure (60b): A graph showing the cumulative unidirectional transfer coefficient by stencil coating.

<p dir="rtl">20 Figure (60c): A graph showing the cumulative unidirectional transfer coefficient by bath coating.</p>

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Figure (60d): A graph showing the cumulative unidirectional transfer coefficient by coating using a sieve.

Figure (61a): A graph showing the ability to control total humidity by spray painting.

Figure (61b): A graph showing the ability to control total humidity using stencil coating.

Figure (61c): A graph showing the ability to control total humidity with paint using a bath.

5 Figure (61d): A graph showing the ability to control total humidity with paint using a sieve.

Figure (62a): A graph showing the peak wetting time.

Figure (62b): A graph showing the bottom of the humidification time.

Figure (63a): A graph showing the facial absorption rate.

Figure (63b): A graph showing the absorption time of Dhahr.

10 Figure (64a): A graph showing the maximum wet radius of the face.

Figure (64b): A graph showing the maximum wet radius.

Figure (65a): A graph showing the speed of spread of a face.

Figure (65b): A graph showing the speed of spread of noon.

Figure (66a): A graph showing the cumulative unidirectional transfer coefficient.

15

Figure (66b): A graph showing the total humidity control ability.

Figure (67a): A graph showing the absorption time of a non-filament finished product.

Figure (67b): A graph showing the hydration time for a semi-finished product before final processing.

Figure (68a): A graph showing the absorption time of a non-filament finished product.

Figure (68b): A graph showing the absorption time of a semi-finished product before final processing.

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Figure (69a): A graph showing the diffusion speed of a non-filament finished product.

Figure (69b): A graph showing the diffusion speed of a semi-finished product before final processing.

Figure (70a): A graph showing the cumulative unidirectional transfer coefficient for a non-filament finished product.

Figure (70b): A graph showing the cumulative unidirectional transfer coefficient for a finished product before processing

<p dir="rtl">5 Final.</p>

Figure (71a): A graph showing the overall humidity control ability of a non-filament finished product.

Figure (71b): A graph showing the overall humidity control ability of a finished product before final processing.

Figure (72a): A graph showing the wetting time using spray paint.

<p dir="rtl">10 Figure (72b): A graph showing the wetting time using stencil paint.</p>

Figure (72c): A graph showing the wetting time with paint using a bath.

Figure (73a): A graph showing the absorption time by spray paint.

Figure (73b): A graph showing the absorption time by stencil coating.

Figure (73c): A graph showing the absorption time by paint using a bath.

<p dir="rtl">15 Figure (74a): A graph showing the speed of spread by spray paint.</p>

Figure (74b): A graph showing the speed of diffusion by stencil painting.

Figure (74c): A graph showing the speed of spreading by paint using a bath.

Figure (75a): A graph showing the cumulative unidirectional transfer coefficient by spray painting.

Figure (75b): A graph showing the cumulative unidirectional transfer coefficient by stencil coating.

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Figure (75c): A graph showing the cumulative unidirectional transfer coefficient by bath coating.

Figure (76a): A graph showing the overall humidity control ability by spray painting.

Figure (76b): A graph showing the overall humidity control ability by stencil coating.

Figure (76c): A graph showing the ability to control the total humidity with a paint using a bath.

5 Figure (77a): A graph showing the hydration time with 1% SFS.

Figure (77b): A graph showing the hydration time with 0.1% SFS.

Figure (78a): A graph showing the absorption time by 1% SFS.

Figure (78b): A graph showing the absorption time by 0.1% SFS.

Figure (79a): A graph showing the speed of propagation by 1% SFS.

10 Figure (79b): A graph showing the speed of propagation by 0.1% SFS.

Figure (80a): A graph showing the cumulative unidirectional transmission coefficient with 1% SFS.

Figure (80b): A graph showing the cumulative unidirectional transmission coefficient by 0.1% SFS.

Figure (81a): A graph showing the total humidity control ability with 1% SFS.

Figure (81b): A graph showing the total humidity control ability with 0.1% SFS.

15

Figure (82a): A graph showing a summary of the peak humidification time.

Figure (82b): A graph showing a summary of the bottom wetting time.

Figure (83a): A graph showing a summary of the facial absorption rate.

Figure (83b): A graph showing a summary of the Dhahr absorption rate.

Figure (84a): A graph showing a summary of the maximum wet radius.

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Figure (84b): A graph showing a summary of the wet radius that appeared.

Figure (85a): A graph showing a summary of the speed of propagation of a face.

Figure (85b): A graph showing a summary of the propagation speed of Dhahr.

Figure (86a): A graph showing a summary of the cumulative unidirectional transfer coefficient.

5 Figure (86b): A graph showing a summary of the total humidity control capacity.

Figure (87): shows the results of bacterial growth.

Figure (88): shows the results of bacterial growth.

Figure (89): shows the results of bacterial growth.

Figure (90): shows the results of bacterial growth.

10 Figure (91): shows the results of bacterial growth.

Figure (92): shows the results of bacterial growth.

Figure (93): Shows a cumulative one-way transfer coefficient versus two fabric washing cycles.

Figure (94): Shows the overall humidity control capacity (OMMC) versus two fabric wash cycles.

Figure (95): Shows the wetting time at the face of the fabric versus the fabric washing cycles.

15 Figure (96): Shows the wetting time at the back of the fabric versus the fabric washing cycles.

Figure (97): shows the absorption rate at the face of the fabric versus the fabric washing cycles.

Figure (98): Shows the absorption rate at the back of the fabric versus the fabric washing cycles.

Figure (99): shows the speed of spread at the face of the fabric versus the fabric washing cycles.

Figure (100): shows the speed of spread at the back of the fabric versus the fabric washing cycles.

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Figure (101): Shows the wet radius at the face of the fabric versus the fabric washing cycles.

Figure (102): Shows the wet radius at the back of the fabric versus the fabric washing cycles.

Figure (103): Shows the percentage decrease in growth of Staphylococcus aureus ATCC 6538 versus fabric washing cycles.

<p dir="rtl">5 Figure (104): Shows the percentage decrease in growth of 4354 Klebisiella pneumoniae ATCC versus fabric washing cycles.</p>

Figure (105): shows a scanning electron microscope image of a fabric sample FAB-01-BATH-B (first site).

Figure (106): shows a scanning electron microscope image of a fabric sample FAB-01-BATH-B (Muscat)

10 second(.

Figure (107): shows a scanning electron microscope image of a fabric sample FAB-01-BATH-B (third view).

Figure (108): shows a scanning electron microscope image of a fabric sample FAB-01-BATH-B (fourth site).

15 Figure (109): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-B (first view).

Figure (110): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-B (second view).

Figure (111): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-B (Muscat 20 Three).

Figure (112): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-B (fourth view).

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Figure (113): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-B (fifth projection).

Figure (114): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-B (sixth site).

<p dir="rtl">5 Figure (115): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-B (seventh site).</p>

Figure (116): shows a scanning electron microscope image of a fabric sample FAB-01-SPRAY-C (first view).

Figure (117): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (Muscat)

10 second(.

Figure (118): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (third view).

Figure (119): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (fourth site).

15 Figure (120): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (fifth projection).

Figure (121): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (first site).

Figure (122): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (Muscat)

20 second(.

Figure (123): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (third view).

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Figure (124): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (fourth site).

Figure (125): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (fifth projection).

<p dir="rtl">5 Figure (126): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (Sixth Muscat).</p>

Figure (127): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (seventh site).

Figure (128): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (Muscat 10 Eighth).

Figure (129): shows a scanning electron microscope image of a fabric sample FAB-01-STEN-C (9th site).

Figure (130): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-B (first site).

15 Figure (131): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-B (second view).

Figure (132): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-B (third view).

Figure (133): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-B (projection 20 four).

Figure (134): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-B (fifth site).

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Figure (135): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-B (Sixth Muscat).

Figure (136): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-B (Seventh Muscat).

<p dir="rtl">5 Figure (137): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (first site).</p>

Figure (138): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (second view).

Figure (139): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (Muscat 10 Three).

Figure (140): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (fourth site).

Figure (141): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (fifth site).

<p dir="rtl">15 Figure (142): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (Sixth Muscat).</p>

Figure (143): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (Seventh Muscat).

Figure (144): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (Muscat 20 Eighth).

Figure (145): shows a scanning electron microscope image of a fabric sample FAB-10-BATH-C (nineth site).

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Figure (146): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (first view).

Figure (147): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (second view).

<p dir="rtl">5 Figure (148): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (third view).</p>

Figure (149): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (fourth view).

Figure (150): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (Muscat 10 Khamis).

Figure (151): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (sixth site).

Figure (152): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (seventh site).

15 Figure (153): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (eighth projection).

Figure (154): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-B (nineth site).

Figure (155): shows a scanning electron microscope image of a fabric sample FAB-10-SPRAY-C.

20 Figure (156): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (first view).

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Figure (157): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (second view).

Figure (158): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (third view).

5 Figure (159): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (fourth view).

Figure (160): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (fifth projection).

Figure (161): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (Muscat)

10 sixth(.

Figure (162): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (seventh site).

Figure (163): shows a scanning electron microscope image of a fabric sample FAB-10-STEN-B (eighth site).

15 Figure (164): shows a scanning electron microscope image of a comparison fabric sample (first site).

Figure (165): shows a scanning electron microscope image of a comparison fabric sample (second view).

Figure (166): shows a scanning electron microscope image of a comparison fabric sample (third view).

Figure (167): shows a scanning electron microscope image of a comparative fabric sample (Square Four).

Figure (168): shows a scanning electron microscope image of a membrane sample FIL-01-BATH-B-01MYL

20 (Muscat first).

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Figure (169): shows a scanning electron microscope image of a membrane sample FIL-01-BATH-B-01MYL

(Second Muscat).

Figure (170): shows a scanning electron microscope image of a membrane sample FIL-01-BATH-B-01MYL

(Third Muscat).

5 Figure (171): shows a scanning electron microscope image of a FIL-01-BATH-B-01MYL membrane sample (fourth site).

Figure (172): Shows a scanning electron microscope image of a FIL-01-BATH-B-01MYL membrane sample (fifth projection).

Figure (173): shows a scanning electron microscope image of a membrane sample FIL-01-BATH-B-01MYL

10 (Muscat sixth).

Figure (174): shows a scanning electron microscope image of a FIL-01-BATH-B-01MYL membrane sample (seventh site).

Figure (175): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 01MYL (first view).

15 Figure (176): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 01MYL (second view).

Figure (177): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 01MYL (third view).

Figure (178): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 20 01MYL (Square Four).

Figure (179): shows a scanning electron microscope image of a membrane sample -FIL-01-SPRAY-B 01MYL (fifth projection).

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Figure (180): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 01MYL (Sixth Muscat).

Figure (181): shows a scanning electron microscope image of a membrane sample -FIL-01-SPRAY-B 01MYL (seventh site).

5 Figure (182): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 01MYL (eighth site).

Figure (183): shows a scanning electron microscope image of a membrane sample -FIL-01-SPRAY-B 007MYL (first site).

Figure (184): shows a scanning electron microscope image of a membrane sample -FIL-01-SPRAY-B

10 007MYL (second hometown).

Figure (185): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 007MYL (third view).

Figure (186): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 007MYL (fourth site).

15 Figure (187): shows a scanning electron microscope image of a membrane sample - FIL-01-SPRAY-B 007MYL (fifth projection).

Figure (188): shows a scanning electron microscope image of a cross-section of a film sample -01-FIL SPRAY-B-O1MYL (first view).

Figure (189): shows a scanning electron microscope image of a cross section of a FIL-01 film sample.

20 SPRAY-B-O1MYL (second hometown).

Figure (190): shows a scanning electron microscope image of a cross-section of a film sample -01-FIL SPRAY-B-O1MYL (third view).

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Figure (191): shows a scanning electron microscope image of a cross-section of a 01 FIL SPRAY B O1MYL film sample (fourth view).

Figure (192): shows a scanning electron microscope image of a cross-section of a 01 FIL SPRAY C 01MYL film sample (first view).

<p dir="rtl">5 Figure (193): shows a scanning electron microscope image of a cross-section of a 01 FIL SPRAY C 01MYL film sample (second view).</p>

Figure (194): shows a scanning electron microscope image of a cross-section of a 01 FIL SPRAY C 01MYL film sample (third view).

Figure (195): shows a scanning electron microscope image of a cross-section of a 01 FIL 10 SPRAY C 01MYL film sample (fourth view).

Figure (196): shows a scanning electron microscope image of a cross-section of a 01 FIL SPRAY C 01MYL film sample (fifth view).

Figure (197): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN B 01MYL film sample (first view).

<p dir="rtl">15 Figure (198): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN B 01MYL film sample (second view).</p>

Figure (199): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN B 01MYL film sample (third view).

Figure (200): shows a scanning electron microscope image of a cross-section of a 01 FIL 20 STEN B 01MYL film sample (fourth view).

Figure (201): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN C 01 MYL film sample (first view).

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Figure (202): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN C 01 MYL film sample (second view).

Figure (203): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN C 01 MYL film sample (third view).

<p dir="rtl">5 Figure (204): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN C 01 MYL film sample (fourth view).</p>

Figure (205): shows a scanning electron microscope image of a cross-section of a 01 FIL STEN C 01 MYL film sample (fifth view).

Figure (206): shows a scanning electron microscope image of a cross-section of a 01 FIL 10 STEN C 01 MYL film sample (sixth view).

Figure (207): shows a scanning electron microscope image of a cross-section of a film sample 10 FIL PATH B 01 MYL (first view).

Figure (208): shows a scanning electron microscope image of a cross-section of a film sample 10 FIL PATH B 01 MYL (second view).

<p dir="rtl">15 Figure (209): shows a scanning electron microscope image of a cross-section of a film sample 10 FIL PATH B 01 MYL (third view).</p>

Figure (210): shows a scanning electron microscope image of a cross-section of a film sample 10 FIL PATH B 01 MYL (fourth view).

Figure (211): shows a scanning electron microscope image of a cross-section of a film sample 10 FIL 20 PATH B 01 MYL (fifth projection).

Figure (212): shows a scanning electron microscope image of a cross-section of a membrane sample 10 FIL PATH B 01 MYL (sixth projection).

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Figure (213): shows a scanning electron microscope image of a cross-section of a membrane sample -10-FIL PATH-B-01-MYL (seventh projection).

Figure (214): shows a scanning electron microscope image of a cross-section of a membrane sample -10-FIL PATH-B-007MEL (first view).

<p dir="rtl">5 Figure (215): shows a scanning electron microscope image of a cross-section of a membrane sample -10-FIL PATH-B-007MEL (second view).</p>

Figure (216): shows a scanning electron microscope image of a cross-section of a film sample -10-FIL PATH-B-007MEL (third view).

Figure (217): shows a scanning electron microscope image of a cross-section of a 10-FIL-10 PATH-B-007MEL film sample (four projections).

Figure (218): shows a scanning electron microscope image of a cross-section of a membrane sample -10-FIL PATH-B-007MEL (fifth view).

Figure (219): shows a scanning electron microscope image of a cross-section of a membrane sample -10-FIL BATH-C-01MYL_cross-section (first view).

15 Figure (220): shows a scanning electron microscope image of a film sample -FIL-10-SPRAY-B 01MYL (first view).

Figure (221): shows a scanning electron microscope image of a film sample -FIL-10-SPRAY-B 01MYL (second view).

Figure (222): shows a scanning electron microscope image of a membrane sample - FIL-10-SPRAY-B 20 01MYL (third view).

Figure (223): shows a scanning electron microscope image of a membrane sample - FIL-10-SPRAY-B 01MYL (fourth site).

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Figure (224): shows a scanning electron microscope image of a 01MYL film sample (fifth site).

Figure (225): shows a scanning electron microscope image of a 01MYL film sample (Sixth Muscat).

5 Figure (226): shows a scanning electron microscope image of a membrane sample (first view).

Figure (227): shows a scanning electron microscope image of a membrane sample (second view).

Figure (228): shows a scanning electron microscope image of a membrane sample 10 (third view).

Figure (229): shows a scanning electron microscope image of a membrane sample (four views).

Figure (230): shows a scanning electron microscope image of a membrane sample (fifth view).

15 Figure (231): shows a scanning electron microscope image of a membrane sample (sixth view).

Figure (232): shows a scanning electron microscope image of a membrane sample

Figure (233): shows a scanning electron microscope image of a membrane sample

Figure (234): shows a scanning electron microscope image of a membrane sample

20 Figure (135): shows a scanning electron microscope image of a membrane sample

Figure (236): shows a scanning electron microscope image of a membrane sample

FIL-10-SPRAY-B-

FIL-10-SPRAY-B-

FIL-BATH-C-01MYL

FIL-BATH-C-01MYL

FIL-BATH-C-01MYL

FIL-BATH-C-01MYL

FIL-BATH-C-01MYL

FIL-BATH-C-01MYL

Melinex comparison (Muscat first).

Melinex comparison (second hometown).

Melinex comparison (third place).

Melinex comparison (Muscat 4).

Mylar comparison (first hometown).

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Figure (237): shows a scanning electron microscope image of a Mylar film sample compared (second view).

Figure (238): shows a scanning electron microscope image of a compared Mylar film sample (third view).

Figure (239): Shows a scanning electron microscope image of a Mylar film sample compared (Section Four).

Figure (240): shows a scanning electron microscope image of a Mylar film sample compared (fifth view).

5 Figure (241): Shows results from optical property measurements on the comparison Mylar sample taken at the face, location 1 (shiny side).

Figure (242): Shows results from optical property measurements on a comparison Mylar sample taken at noon, location 2 (rougher, less shiny side).

Figure (243): shows results from optical property measurements on the Melinex comparison sample taken 10 at the face, location 1.

Figure (244): Shows results from optical property measurements on a comparison Melinex sample taken at noon, location 2.

Figure (245): Shows results from optical property measurements on sample FIL-10-SPRAY-B 01MYL, taken at the face, location 1.

15 Figure (246): Shows results from optical property measurements on sample FIL-10-SPRAY-B 01MYL, taken at noon, location 2.

Figure (247): Shows results from optical property measurements on sample FIL-10-SPRAY-B 01MYL, taken at the face, location 1.

Figure (248): shows results from optical property measurements on the FIL-10-SPRAY-B sample.

20 01MYL, taken at noon, location 2.

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Figure (249): shows results from optical property measurements on sample FIL-10-SPRAY-B

01MYL, taken at the face, position 1.

Figure (250): Shows results from optical property measurements on sample FIL-10-SPRAY-B 01MYL, taken at noon, location 2.

5 Figure (251): Shows results from optical property measurements on sample FIL-10-SPRAY-B 01MYL, taken at the face, location 1.

Figure (252): shows results from optical property measurements on the FIL-10-SPRAY-B sample.

01MYL, taken at noon, location 2.

Figure (253): shows results from optical property measurements on the FIL-10-STEN-B sample.

10 01MYL, taken at the face, position 1.

Figure (254): shows results from optical property measurements on the FIL-10-STEN-B sample.

01MYL, taken at noon, location 2.

Figure (255): shows results from optical property measurements on the FIL-10-STEN-C sample.

01MYL, taken at the face, position 1.

15 Figure (256): shows results from optical property measurements on the FIL-10-STEN-C sample.

01MYL, taken at noon, location 2.

Figure (257): shows results from optical property measurements on sample FIL-10-BATH-B

01MYL, taken at the face, position 1.

Figure (258): shows results from optical property measurements on sample FIL-10-BATH-B

20 01MYL, taken at noon, location 2.

Figure (259): shows results from optical property measurements on sample FIL-10-BATH-B

007MEL, taken at the face, position 1.

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Figure (260): Shows results from optical property measurements on sample FIL-10-BATH-B 007MEL, taken at noon, location 2.

Figure (261): Shows results from optical property measurements on sample FIL-10-BATH-C 01MYL, taken at the face, location 1.

5 Figure (262): Shows results from optical property measurements on sample FIL-10-BATH-C 01MYL, taken at noon, location 2.

Figure (263): Shows results from optical property measurements on sample FIL-10-BATH-B 01MYL, taken at noon, location 2.

Figure (264): shows results from optical property measurements on sample FIL-10-BATH-B

<p dir="rtl">10 01MYL, taken at noon, location 2.</p>

Figure (265): shows a scanning electron microscope image of a cross-section of a membrane sample from a cross-section

.FIL-01-SPRAY-B-O1MYL

Figure (266): shows a scanning electron microscope image of a cross-section of a membrane sample from a cross-section

.FIL-01-SPRAY-B-O1MYL

<p dir="rtl">15 Figure (267): shows a scanning electron microscope image of a cross-section of a membrane sample from a cross-section</p>

.FIL-01-SPRAY-B-O1MYL

Figure (268): shows a scanning electron microscope image of a cross-section of a membrane sample from a cross-section

.FIL-01-SPRAY-B-O1MYL

Figure (269): shows the results of a cumulative unidirectional transmission coefficient for natural fibers.

<p dir="rtl">20 Figure (270): shows the overall moisture control ability of natural fibers.</p>

While the drawings shown above illustrate currently disclosed models, other models are also expected to exist, as mentioned in the discussion. This invention provides illustrative examples, for example:

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Restriction. Many other modifications and models can be devised by those skilled in the art that fall within the scope and scope of the principles of the models currently disclosed.

Detailed description:

Herein, methods for producing solutions of mixtures of pure, amplifiable silk protein fragments are presented

<p dir="rtl">5 High grade which can be used to coat part of a textile or can be formed into usable fibers to be woven into yarn. The solution is generated from intact, pure, raw silk protein material and processed to remove any sericin and obtain the desired average molecular weight (MW) and polydispersity of the fragment mixture. The choice of method parameters can be modified to obtain distinct properties of final silk protein fragments depending on the intended use. The solution is the fragments</p>

<p dir="rtl">10 The resulting final product is pure silk protein fragments and water at a concentration of ppm to undetectable levels of process contaminants.</p>

The concentration, size and polydispersity of the silk protein fragments in solution can also be adjusted depending on the desired application and pressure requirements. In one embodiment, the aqueous solution of pure silk fibroin-based protein fragments in solution is essentially devoid of sericin, and has a molecular weight

<p dir="rtl">15 Its average weight ranges between about 6 and 16 kDa, and its polydispersity ranges between about 1.5 and 3.0. In one embodiment, the aqueous solution of pure silk fibroin-based protein fragments in solution is mainly devoid of sericin, has an average molecular weight of between 17 and 38 kDa, and has a polydispersity of between about 1.5 and 3.0. In one embodiment, the aqueous solution of pure silk fibroin-based protein fragments in solution is essentially free of</p>

<p dir="rtl">20 Sericin, has an average molecular weight that ranges between 39 and 80 kilodaltons, and has a polydispersity that ranges between about 1.5 and 3.0. In an embodiment, the solution may be used to produce products, such as silk gels with different gel properties by varying the water concentration/content, or sold as a raw ingredient to the consumer market.</p>

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As used herein, the terms “primarily sericin-free” or “primarily sericin-free” refer to silk fibers from which the sericin protein has mostly been removed. In an embodiment, the mainly sericin-free silk fibroin refers to a silk fibroin with between about 0.01 and 10.0% (w/w) sericin. In an embodiment, the silk fibroin

<p dir="rtl">5 Mainly sericin-free refers to silk fibroin having between about 0.01 and 9.0% (w/w) sericin. In an embodiment, mainly sericin-free silk fibroin refers to silk fibroin having between about 0.01 and 8.0% (w/w) sericin. In an embodiment, silk fibroin devoid of mainly sericin refers to silk fibroin with between about 0.01 and 7.0% (w/w) sericin. In an embodiment, Silk fibroin</p>

<p dir="rtl">10 Mainly sericin-free refers to silk fibroin having between about 0.01 and 6.0% (w/w) sericin. In an embodiment, mainly sericin-free silk fibroin refers to silk fibroin having between about 0.01 and 5.0% (w/w) sericin. In an embodiment, silk fibroin devoid of mainly sericin refers to silk fibroin with between about 0.01 and 4.0% (w/w) sericin. In an embodiment, Silk fibroin</p>

<p dir="rtl">15 Mainly sericin-free refers to silk fibroin having between about 0.05 and 4.0% (w/w) sericin. In an embodiment, mainly sericin-free silk fibroin refers to silk fibroin having between about 0.0% (w/w) sericin. 1 and 4.0% (w/w) sericin. In an embodiment, silk fibroin devoid of mainly sericin refers to silk fibroin with between about 0.5 and 4.0% (w/w) sericin. In an embodiment. , the silk fibroin</p>

<p dir="rtl">20 Mainly sericin-free refers to silk fibroin having between about 1.0 and 4.0% (w/w) sericin. In an embodiment, mainly sericin-free silk fibroin refers to silk fibroin having between about 1.5% (w/w) sericin. and 4.0% (w/w) sericin. In an embodiment, silk fibroin devoid of mainly sericin refers to silk fibroin with between about 2.0 and 4.0% (w/w) sericin. In an embodiment, Free silk fibroin</p>

<p dir="rtl">25 Mainly sericin refers to silk fibroin containing between about 2.5 and 4.0% (w/w) sericin. In an embodiment, silk fibroin devoid of mainly sericin refers to fibrin</p>

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Silk having a sericin content between about 0.01 and 0.1% (w/w). In an embodiment, silk fibroin mainly free of sericin refers to silk fibroin having a sericin content under about 0.1% (w/w). In an embodiment Basically, sericin-free silk fibroin refers to silk fibroin with a sericin content under about 0.05% (w/w).

<p dir="rtl">5 In one embodiment, when a silk source is added to an actual aqueous solution (100°C) of sodium carbonate for a treatment time between about 30 and 60 minutes, a degumming loss of between about 26 and 31% by weight is obtained.</p>

As used herein, the term “mainly homogeneous” can refer to an aqueous solution of pure silk fibroin-based protein fragments distributed with a normal distribution around the weight

<p dir="rtl">10 Specific molecular. As used herein, the term “mainly homogeneous” can refer to a uniform distribution of an additive, for example, vitamin C, throughout the composition of the present invention.</p>

As used herein, the term “primarily free of inorganic residues” means a composition exhibiting 0.1% (w/w) or less residues. In an embodiment, the term “primarily free of inorganic residues” means a composition exhibiting 0.1% (w/w) or less residues. Building units 0.05% (w/w)

<p dir="rtl">15 or less. In an embodiment, the composition is essentially free of inorganic residues to a composition exhibiting 0.01% (w/w) residues or less. In an embodiment, the amount of inorganic residues is between 0 ppm (undetectable or “undetectable”). ND” and 1000 ppm. In one embodiment, the amount of inorganic residues is ND to about 500 ppm.</p>

Million. In one embodiment, the amount of inorganic residues is ND to about 400 pp

<p dir="rtl">20 Million. In one embodiment, the amount of inorganic residues is ND to about 300 ppm. In one embodiment, the amount of inorganic residues is ND to about 200 pp</p>

Million. In one embodiment, the amount of inorganic residues is ND to about 100 pp

Million. In an embodiment, the amount of inorganic residues is between 10 and 1000 ppm.

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As used herein, the term “primarily organic residue-free” means a composition exhibiting 0.1% (w/w) or less residues. In an embodiment, a composition that exhibits mainly organic residues is 0.1% (w/w) or less. 0.05% (w/w) or less. In an embodiment, essentially devoid of organic residues to a composition exhibiting residues

<p dir="rtl">5 0.01% (w/w) or less. In an embodiment, the amount of organic residues is between zero</p>

ppm (non-detectable or “ND”) and 1,000 ppm. In an embodiment, the amount of organic residues is ND to about 500 ppm. In an embodiment, the amount of organic residues is ND to about 400 ppm In an embodiment, the amount of organic residues is ND to about 300 ppm

<p dir="rtl">10 ND organic building blocks to about 200 ppm. In one embodiment, the amount of organic residues is ND to about 100 ppm. In an embodiment, the amount of organic residues is between 10 and 1000 ppm.</p>

The compositions of the present invention show “biocompatibility” meaning that the compositions are compatible with living tissue or a living system by not being toxic, causing injury, or physiologically reactive, and not causing immune rejection.

<p dir="rtl">15 Such biocompatibility can be demonstrated by participants topically applying the compositions of the present invention to their skin for an extended period of time. In an embodiment, the extended period of time is approximately 3 days. In an embodiment, the extended period of time is approximately 7 days. In an embodiment, the period of time is approximately 14 days. In an embodiment, the period of time is approximately 21 days. In an embodiment, the period of time is approximately 30 days. In a model, the period is chosen</p>

<p dir="rtl">20 Extending a period of time of approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 months, and indefinitely.</p>

That the compositions of the present invention are “mildly hypoallergenic” means that they are relatively unlikely to cause an allergic reaction. This slight cause of sensitivity can be demonstrated by participants topically applying compositions of the present invention to their skin for an extended period of 25 minutes. In an embodiment, the extended period of time is approximately 3 days. In an embodiment, the period

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The period of time is about 7 days. In an embodiment, the period of time is approximately 14 days. In an embodiment, the period of time is approximately 21 days. In an embodiment, the period of time is approximately 30 days. In an embodiment, the period of time is chosen from a set consisting of approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 months, indefinitely.

<p dir="rtl">5 As used herein, the terms “washable” and “showing washability” mean that the silk-coated fabric of the present invention can be washed without shrinking, fading, or the like.</p>

As used herein, the term “nonwovens” refers to a flexible woven material consisting of a network of natural or synthetic fibers referred to as thread or yarn. In some form, it can

<p dir="rtl">10 The use of textiles to manufacture clothing, shoes, and bags. In an embodiment, the textiles may be used to manufacture rugs, upholstered furniture, shutters, towels, tablecloths, beds, and other flat surfaces. In one example the textiles can be used to make flags, backpacks, tents, netting, handkerchiefs, balloons, bat wings, sails, and parachutes.</p>

As used herein, the term “hand feel” refers to the feel of the fabric, which 15 is further described as a sensation of softness, wrinkle, dryness, silkiness, and combinations thereof. It is also indicated

refers to the hand-feel of the fabric as being "flexible without creasing." Fabric with a hard hand-feel is considered rough, rough, and generally less comfortable for the wearer. A fabric with a soft hand feel is fluid and soft, such as silk or fine wool, and is generally more comfortable for the wearer. The hand texture of a fabric can be determined by comparing it to sets of fabric samples, or using methods such as

<p dir="rtl">20 Kawabata Evaluation System (KES) or methods of confirming fabric quality by simple tests (FAST).</p>

Behera and Hari, India. J. Fiber &amp; Textile Res., 1994,19, 168-71

As used herein, “yarn” refers to a single- or multi-fiber construction.

As used herein, “bath painting” includes painting a fabric in a batch manner, by dipping it in a bath, and by immersing it in a bath.

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In an embodiment, the silk coating is applied using a bath process, a sieve (or stencil) process, a spray process, a silk foam-based process, and a dolphin-based process.

In an embodiment, the fiber or yarn includes a synthetic fiber or yarn, including polyester, Mylar, cotton, nylon, polyester-polyurethane copolymer, aryon, acetate, aramide (polyamide).

<p dir="rtl">5 aromatic), acrylic, angio (polylactide), lurex (polyamide-polyester) or olefin (polyethylene-polypropylene), and combinations thereof.</p>

In an embodiment, the fiber or yarn includes natural fibers or yarn, including alpaca sheep fibre, alpaca sheep hair, alpaca sheep wool, llama fibre, lama hair, llama wool, laman wool, cotton, sheep wool or soft goat wool, and Kharif, and wool Kharif.

<p dir="rtl">10 In an embodiment, a water-soluble silk coating can be used as an adhesive or bonding material to attach particles to fabrics or to bond fabrics. In an embodiment, a product comprising a fabric bonded to another fabric can be made using a silk coating. In an embodiment, a product can be made that includes fabric particles bonded to the fabric using a silk-screen adhesive.</p>

In an embodiment, the coating is applied to a product comprising fabric at the yarn level. In an example,

<p dir="rtl">15 The paint is applied to the level of the canvas. In an embodiment, the coating has a thickness selected from a set consisting of about 5, 10, 15, 20, 25, 50, 100, 200, 500 nanometers, and about 1, 5, 10, 20 micrometers. In an embodiment, the coating has a thickness range selected from a set consisting of 200500 nm, about 1-2, about 2-5, about 5-10, about 10-20 micrometers.</p>

In an embodiment, the fiber or yarn is treated with polymer, such as polyglycolide (PGA), compounds

<p dir="rtl">20 polyethylene glycol, copolymer of glycolide, copolymer of glycolide-lactide (PGA/PLLA), copolymer of glycolide/tarimethylene carbonate (PGA/TMC), polylactide (PLA) compounds, homologous copolymer of PLA; Poly-L-lactide (PLLA), Poly-DL-L-Lactide (PDLLA), Copolymer L-Lactide/DL-Lactide, Copolymer of PLA, Copolymer Lactide/T-R Methyl Glycolide, Copolymer Lactide/ Trimethylene carbonate,</p>

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Co-polymer lactide/δ-valerolactone, Polymer co-lactide, ε-caprolactone, polydipsipeptide complexes, Polymer copolymer PLA/polyethylene oxide, poly-1,4-dioxane-2,5-dione compounds with substitution at both positions. 3, 6 asymmetric, poly-β-hydroxybutyrate (PHBA), copolymer β/PHBA- hydroxyvalate (PHBA/HVA), poly-β-

<p dir="rtl">5 Hydroxypropionate (PHBA), Poly-ρ-Dioxanone (PDS), Poly-δ- Valerolactone, Poly-ε-Caprolactone, Copolymer Methyl Methacrylate -N-Vinylpyrrolidine, Polyester Amide Compounds, Oxalic Acid Polyester Compounds Polydihydroprene, Polyalkyl-2-cyanoacrylates, Polyurethanes (PU), Polyvinyl alcohols (PVA), Poly-β-malic acid (PMLA) polypeptides, Poly-β-alkanoic acids, Polyvinyl alcohol (PVA),</p>

<p dir="rtl">10 Polyethylene oxide (PEO), chitinous polyart, polyethylene, polypropylene, polyacetal, polyamides, polyesters, polysulfone, polyether ether ketone, polyethylene terephthalate, polycarbonate, polyaryl ether ketone, and poly ketone Ether ketone.</p>

In an embodiment, the surface of the silk coating can be a modified silk blue art that ranges in size from nanometers to micrometers.

<p dir="rtl">15 The “appearance” criterion is met by any of the following: a change in a surface property of the textile, the silk coating filling in gaps where the yarns intersect or the silk coating blurring or concealing the fabric.</p>

In an embodiment, a silk-based fragment or protein solution can be used to coat at least a portion of a fabric that can be used to produce textiles. In an embodiment, a solution of silk-based fragments or protein can be transcribed into yarn that can be used for fabric in textiles. In an embodiment, a solution may be used

<p dir="rtl">20 Fragments or protein based silk for coating fibers. In an embodiment, the invention provides a product comprising a silk-based fragment or protein solution for coating at least a portion of a fabric or textile. In an embodiment, the invention provides a product comprising a silk-based fragment or protein solution for coating yarn. In an embodiment, the invention provides a product comprising a silk-based fragment or protein solution for coating fibers.</p>

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In an embodiment, the solution of the present invention is brought into contact with an additive, such as a therapeutic agent and/or molecule. In an embodiment, the molecules include, but are not limited to, antioxidants and enzymes. In an embodiment, the particles include, but are not limited to, ceramics, ceramic particles, metals, and particles

Mineral, polymeric particles, inorganic particles, organic particles, selenium, derivatives

<p dir="rtl">5 Ubiquinone, thiol-based antioxidants, antioxidants containing sugars, polyphenolic compounds, plant extracts, caffeic acid, apigenin, pycnogenol, arbuterol, folic acid, vitamin B12, vitamin B6, vitamin B3, vitamin E, vitamin C, and derivatives thereof, Vitamin D, Vitamin A, Astaxazine, Lutein, Lycopene, Essential Fatty Acids (Omega 3 and 6), Iron, Zinc, Magnesium, Flavonoids (Soy, Turmeric, Silymarin, Picnonginol), Growth Factors, Alcohol, Acid</p>

<p dir="rtl">10 Hyaluronic acid, extracellular matrix propionate, cells, nucleic acids, biomarkers, biological tracers, zinc oxide, benzoyl peroxide, retinoids, titanium, allergens in a known dose (for treatment of allergic provocation), essential oils including, but not limited to, oil Lemongrass or</p>

Frankincense gravel oil, and perfumes. Therapeutic agents include, but are not limited to, small molecules, drugs, proteins, peptides, and nucleic acids. In an embodiment, the solution of the invention is in contact with the current

<p dir="rtl">15 With an allergen in a known quantity before forming a product. Allergens include, but are not limited to, milk, beans, peanuts, tree nuts, fish, snails, soybeans, and wheat. Known doses of an allergen loaded within a silk product can be released at a known rate of</p>

D: Study of controlled exposure to an allergen, testing, and treatment of allergic triggers.

In an embodiment, the solution of the present invention is used to produce a product with fine needles by methods

<p dir="rtl">20 A standard known to a person in the art for the controlled delivery of therapeutic molecules or agents to or through the skin.</p>

As used herein, the term “fibroin” includes silkworm fibroin and an insect or spider silk protein. In an embodiment, fibroin is obtained from Bombyx mori. In an embodiment, a spider silk protein is selected from a pool consisting of coiled silk. Phase gland silk

25 larva), cocoon silk (cocoon gland silk), cocoon silk, long-tubule silk), silk

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Drawn non-viscous (ampullary gland silk), attached silk thread (pear-shaped gland silk), viscous silk core fibers (flagellate gland silk), and sticky outer silk fibers (clumped gland silk).

Figure (1) process flow chart showing different models for producing aqueous solutions from protein fragments

<p dir="rtl">5 Based on pure silk fibroin (SPFs) of the current invention. It should be understood that not all of the steps described are necessarily required for the manufacture of all silk solutions of the present invention. As shown in Figure 1, step A can use cocoons (heat-treated or untreated), silk fibers, silk powder, or spider silk as the silk source. If starting from raw silk cocoons from Bombyx mori, the cocoons can be cut into small pieces, for example approximately equal 10 sized pieces, and in Step B1, the silk can then be extracted and rinsed to remove any sericin, in Step C1a. These produce raw silk that is mainly free of sericin. In an embodiment, water is heated to a temperature between 84 and 100°C (ideally boiling water) and then sodium carbonate (Na2CO3) is added to the boiling water until completely dissolved. The raw silk is added to the Na2CO3/boiling water (100°C) solution . Submerge it for approximately 15-90 minutes, as boiling it for...</p>

<p dir="rtl">15 Longer ones produce smaller silk protein fragments. In an embodiment, the volume of water is about 0.4 times the weight of the raw silk, and the volume of Na2CO3 is about 0.848 times the weight of the raw silk. In an embodiment, the volume of water is 0.1 times the weight of raw silk and the volume of Na2CO3 is maintained at 2.12 g/L. This was illustrated in Figures (38a, 38b) where the mass of silk (x-axis) differed in the same volume of extraction solution (i.e. the same volume of water and the same concentration of Na2CO3), which</p>

<p dir="rtl">20 It achieves sericin removal (mainly sericin-free) as indicated by a decrease in total silk mass from 26 to 31% (y-axis). Accordingly, the Na2CO3 solution dissolved in water is filtered and excess Na2CO3/water solution is removed from Silk fibroin fibers (e.g. squeezing the extracted fibroin by hand, squeezing it using a machine, etc.). The resulting extracted silk fibroin is rinsed with warm to hot water to remove any remaining adsorbed sericin or contaminants, usually at 25°C, ranging from approximately 40 to 80°C, with the volume of water changed at least once (repeated several times).</p>

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10

15

20

(Mart upon request). The resulting extracted silk fibroin is mainly sericin-depleted silk fibroin. In an embodiment, the resulting extracted silk fibroin is rinsed with water at a temperature of about 60°C. In an embodiment, the volume of rinse water per cycle is between 0.1 and 0.2 liters per weight of raw silk It may be useful to agitate, swirl or rotate the rinse water to maximize the rinsing effect. After rinsing, excess water is removed from the extracted silk fibroin (eg by squeezing the extracted fibroin by hand). Or using a machine. Alternatively, methods known to a person skilled in the art, such as pressure, temperature, or other reagents, or combinations thereof, may be used for the purpose of extracting sericin. Alternatively, the silk gland (100% sericin-free silk protein) can be removed directly from the worm. This will produce a liquid silk protein that, without any modification of the protein structure, is free of sericin.

The extracted fibroin fibers are then allowed to dry completely. Figure 3: A photograph showing dried extracted silk fibroin. Once dried, the extracted silk fibroin is dissolved using a solvent added to the silk fibroin at a temperature between ambient and boiling, in step C1b. In an embodiment, the solvent is A solution of lithium bromide (LiBr) (boiling at 140°C). Alternatively, the extracted fibroin fibers are not dried but moistened and placed in a solvent, whereupon the concentration of the solvent can be changed to obtain a concentration similar to that when dried silk is added to the solvent. The final concentration of LiBr solvent can range from 0.1 to 9.3 M. Figure (39) is a table summarizing the molecular weights of dissolved silk from different concentrations of lithium bromide (LiBr) and from different extraction and dissolution volumes. Complete dissolution of the extracted fibroin fibers can be achieved by changing the weight and treatment temperature along with the concentration of the dissolution solvent. Other solvents may be used including, but not limited to, phosphoric acid phosphate, calcium nitrate, calcium chloride solution, or other concentrated aqueous solutions of inorganic salts. To ensure complete dissolution, the silk fibers must be completely immersed in a pre-heated solvent solution and then maintained at a temperature ranging between approximately 60 and 140°C for 1-168 hours. In an embodiment, the fibers must be immersed

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The silk is completely immersed in the solvent solution and then placed in a drying oven at a temperature of about 100 °C for about 1 hour.

The temperature at which the extracted silk fibroin is added to the LiBr solution (or vice versa) has an effect on the time required for complete dissolution of the fibroin and on the molecular weight and resulting polydispersity.

<p dir="rtl">5 For the final SPF blend solution. In an embodiment, the solvent solution concentration is ≥ 20% w/v. In addition, stirring may be used during insertion or thawing to facilitate thawing at degrees</p>

Heat and Turkish are different. The temperature of the LiBr solution will provide control over the molecular weight and resulting polydispersity of the mixture of silk protein fragments. In an embodiment, a higher temperature will more rapidly melt the silk providing enhanced amplification of the process and more quantitative production of the silk solution. In one embodiment, 10 by using a LiBr solution heated to a temperature between 80 and 140° the time required in the oven to achieve complete dissolution is reduced. Changing the time and temperature at 60°C or higher of the desolvation solvent will modify and control the MW and polydispersity of the SPF mixture solutions composed of the original molecular weight.

Silk fibroin protein.

15

Alternatively, whole cocoons can be placed directly in a solvent, such as LiBr, avoiding extraction, in step B2. This requires subsequent filtration of the silkworm particles from the silk and solvent solution and removal of sericin using methods known in the art to separate hydrophilic and hydrophilic proteins such as columnar separation and/or chromatography, ion exchange, chemical precipitation by salt and/or pH, and/or Or enzymatic digestion, filtration, or extraction, all methods are common examples without adherence to standard protein separation methods, in step C2. Alternatively, untreated cocoons can be placed

<p dir="rtl">20 By thermally removing the silkworms in a solvent such as LiBr, bypassing the extraction. The mentioned methods can be used</p>

by separating the sericin, maintaining the advantage that non-heat-treated cocoons will contain significantly less worm residue.

Membrane separation can be used to remove the desolvation solvent from a solution of dissolved fibroin protein fragments by membrane separation of the solution versus a volume of water, in step E1. Pre-filtration before membrane separation 25 helps remove any residue (i.e. silkworm residue) from the silk and LiBr solution, in step D. In one

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Examples: A 3 or 5 µm filter at a flow rate of 200-300/min is used to filter between 0.1 and 1.0% of a Silk-LiBr solution prior to membrane separation and custom potential concentration. The method disclosed herein, and as previously described, is to use time and/or temperature to reduce the concentration from 9.3 M of the LiBr solution to between 0.1 and 9.3 M to facilitate filtration.

<p dir="rtl">5 and subsequent membrane separation, especially when the establishment of a scalable process method is taken into account. Alternatively, without the use of additional time or temperature, the 9.3 LiBr molar solution of silk protein fragments can be diluted with water to facilitate filtration and membrane separation of the residue. Dissolution result at desired filtration time and temperature LiBr solution - silk protein fragments that are stable when stored at room temperature and free of particles of known MW and polydispersity. It is useful to change the separation water</p>

<p dir="rtl">10 membrane regularly until the solvent is removed (e.g. water changes every 1, 4 hours, and then every 12 hours for a total of 6 water changes). The total number of water volume changes can vary depending on the resulting concentration of solvent used to dissolve and break up the silk protein. After membrane separation, the final silk solution can also be filtered to remove any remaining residue (i.e. silkworm residue).</p>

Alternatively, TFF can be used, which is a fast and efficient separation method

<p dir="rtl">15 and purify biomolecules to remove the solvent from the resulting dissolved ferroin solution, in step E2. TFF delivers a highly pure aqueous silk protein fragment solution and enables the process to be scaled up so that large volumes of solution can be produced in a standardized, repeatable manner. The solution of silk and LiBr can be diluted before TFF (20% silk) up to 0.1% silk in either water or LiBr). Pre-filtration as mentioned before before treatment with TFF can maintain filter efficiency and potentially avoid the production of layers.</p>

<p dir="rtl">20 A separation of silk gel on the filter surface due to the presence of residue particles. Pre-filtration before TFF is also useful to remove any remaining residue (i.e. silkworm residue) from the silk and LiBr solution which could cause self- or long-term gelation of the resulting solution consisting only of water, in step D. Single-pass or rotary TFF can be used To produce silk protein fragment-water solutions with a silk concentration between 0.1 and 30.0% (more preferably between 0.1 and 6.0% silk). Membranes may be required.</p>

<p dir="rtl">25 TFF has different separation size depending on concentration, molecular weight, and polydispersity</p>

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It is required to mix the silk protein fragments in solution. Membranes of 1 to 100 kDa may be necessary for silk solutions of varying molecular weights produced, for example, by varying the boiling time of the extraction solution or the time and temperature of the dissolution solution (e.g., LiBr). In one embodiment, TFF5 or 10 kDa membrane was used to purify the mixture of silk protein 5 fragments and to produce the final desired silk-to-water ratio.

In addition, single-pass TFF, TFF, and other methods known in the art, such as a descending film evaporator, can be used to concentrate the solution after the desolvation solvent (e.g. LiBr) has been removed (with a desired yield concentration of 0.1 to 30% sil). This can be used As an alternative to known arthritic HFIP concentration methods for producing a water-based solution, a macroporous membrane can also be used to filter small silk protein 10 fragments and to produce a solution of silk with a molecular weight greater than and/or without polydispersity values that are more similar.

Figure (37) is a table summarizing the molecular weights of some examples of silk protein solutions of the present invention. The processing conditions for the silk protein solution were as follows: extraction at 100°C for 20 minutes, rinsing at room temperature, LiBr in an oven at 60°C for 4-6 hours. Figures (40-49) also show the treatment of extraction time, dissolution conditions in LiBr, TFF treatment, and balance.

<p dir="rtl">15 molecular, and the resulting representative polydispersions. These examples are not limiting, but instead illustrate the strength of specific determinants of solutions of silk fragments of a given molecular weight.</p>

An experiment was conducted to detect LiBr and Na2CO3 using an HPLC system equipped with an evaporative light scattering (ELSD) detector. Calculations were performed by linear regression of the resulting peak areas of the decomposition product plotted against concentration. More than one sample was used for a number of formulas of the present invention to prepare and analyze the sample. Generally, four samples with different formulas were weighed directly into a standard 10 mL vial.

The analytical method developed for the quantitative determination of Na2SO3 and LiBr in silk protein formulations was found to be linear in the range between 10 and 165 μg/ml, and has an RSD of 2% and 1% injection accuracy, and 0.38% and 0.19% retention time for sodium carbonate and lithium bromide. , respectively.

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The analytical method for the quantitative determination of sodium carbonate and lithium bromide can be applied to formulas

Silk protein.

The final silk protein fragment solution, as shown in Figure 4, is pure silk protein fragments and water at ppm concentrations up to undetectable levels of particulates and/or

<p dir="rtl">5 Process pollutants, including LiBr and Na2CO3. Figures (34, 35) are tables summarizing the concentrations of LiBr and Na2CO3 in solutions of the present invention. In Figure (34), the processing conditions include extraction at 100°C for 60 minutes, rinsing at 60°C, dissolving in LiBr at 100°C in an oven at 100°C for 60 minutes, TFF conditions include the difference in pressure and the number of filtration volumes in membrane separation in Figure (35). Treatment conditions include boiling at 100°C for 60 minutes.</p>

<p dir="rtl">10 Rinse at 60°C, dissolve in LiBr in an oven at 60°C for 4-6 hours. In an embodiment, the SPF composition of the present invention does not dissolve in an aqueous solution due to crystallization of the protein. In an embodiment, the SPF composition of the present invention is dissolved in an aqueous solution. In an embodiment, the SPFs of the composition of the present invention comprise a crystalline portion of about two-thirds and an amorphous region of about one-third. In an embodiment, the SPFs of the composition of the present invention comprise a crystalline portion of about one-half and an amorphous region of about</p>

<p dir="rtl">15 Half. In an embodiment, the SPFs of the composition of the present invention comprise a 99% crystalline portion and a 1% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 95% crystalline portion and a 5% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 90% crystalline fraction and a 10% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise an 85% crystalline portion and a 15% amorphous region. In an embodiment, the SPFs of the composition of the present invention include</p>

<p dir="rtl">20 It contains 80% crystalline part and 20% amorphous part. In an embodiment, the SPFs of the composition of the present invention comprise a 75% crystalline portion and a 25% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 70% crystalline portion and a 30% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 65% crystalline portion and a 35% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 60% crystalline portion and a 40% amorphous region. In an example,</p>

<p dir="rtl">25 The SPFs of the composition of the present invention comprise a 50% crystalline portion and a 50% amorphous zone. in</p>

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In one embodiment, the SPFs of the composition of the present invention comprise a 40% crystalline portion and a 60% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 35% crystalline portion and a 65% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 30% crystalline portion and a 70% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a crystalline portion

<p dir="rtl">5 25% and 75% amorphous area. In an embodiment, the SPFs of the composition of the present invention comprise a portion</p>

20% crystalline and 80% amorphous area. In an embodiment, the SPFs of the composition of the present invention comprise a 10% crystalline fraction and an 85% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 10% crystalline fraction and a 90% amorphous region. In an embodiment, the SPFs of the composition of the present invention comprise a 5% crystalline fraction and a 95% amorphous region. In an embodiment, SPFs include:

<p dir="rtl">10 The composition of the present invention has a 1% crystalline portion and a 99% amorphous portion.</p>

A unique feature of the SPF compositions of the present invention is their stability during storage (they do not gel slowly or self-gel when stored in aqueous solution, there is no agglomeration of fragments so their molecular weight does not increase over time), from 10 days to 3 years depending on storage conditions, ratio The percentage of silk, the number of charges and the shipping conditions. Additionally the pH can be adjusted to extend the shelf life 15 and/or support the shipping conditions by preventing premature folding and agglomeration of the silk. In one embodiment, the formulation

The SPF solutions of the present invention have a storage stability of up to two weeks at room temperature (RT). In an embodiment, the SPF solution composition of the present invention has storage stability for up to four weeks at room temperature (RT). In an embodiment, the SPF solution composition of the present invention has storage stability for up to six weeks at room temperature (RT). In an embodiment, the SPF lotion composition

<p dir="rtl">20 The present invention has a storage stability of up to eight weeks at room temperature (RT). In an example,</p>

The SPF solution composition of the present invention has a storage stability of up to ten weeks at room temperature (RT). In an embodiment, the SPF solution composition of the present invention has storage stability for up to twelve weeks at room temperature (RT). In an embodiment, the SPF solution composition of the present invention has a shelf stability of up to fifty-two weeks at room temperature (RT).

<p dir="rtl">25 The following Table (1) shows the results of the storage stability test for the SPF formulation models of the present invention.</p>

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Table 1: Storage stability of the SPF formulations of the present invention

<tr><td><p dir="rtl">The time of formation of all</p></td><td><p dir="rtl">Temperature</p></td><td><p dir="rtl">% for silk</p></td></tr><tr><td><p dir="rtl">4 Weeks</p></td><td><p dir="rtl">Room temperature</p></td><td><p>2</p></td></tr><tr><td><p dir="rtl">&lt; 9 weeks</p></td><td><p dir="rtl">4 Degrees Celsius</p></td><td><p>2</p></td></tr><tr><td><p dir="rtl">4 Weeks</p></td><td><p dir="rtl">Room temperature</p></td><td><p>4</p></td></tr><tr><td><p dir="rtl">&lt; 9 weeks</p></td><td><p dir="rtl">4 Degrees Celsius</p></td><td><p>4</p></td></tr><tr><td><p dir="rtl">Two weeks</p></td><td><p dir="rtl">Room temperature</p></td><td><p>6</p></td></tr><tr><td><p dir="rtl">&lt; 9 weeks</p></td><td><p dir="rtl">4 Degrees Celsius</p></td><td><p>6</p></td></tr>

The water-silk fragment solution of the present invention can be sterilized by standard methods in the art not limited to filtration, heating, exposure to radiation, or an electron beam. It is expected that a mixture of silk protein fragments, because of its shorter protein polymer length, will withstand sterilization better than intact silk protein solutions reported in the art. In addition, it can sterilize silk products

<p dir="rtl">5 Produced from the SPF mixtures mentioned herein as appropriate for use.</p>

Figure 2: A process flow chart illustrating various parameters that can be modified during the process of producing a solution of silk protein fragments of the present invention during the extraction and dissolution steps. The choice of method parameters can be modified to obtain distinct final solution characteristics depending on the intended use, e.g., molecular weight and dispersion. Multimeter must be understood that it is not needed

<p dir="rtl">10 necessarily follow all the steps described in order to manufacture all the silk solutions of the present invention.</p>

In an embodiment, a process for producing a silk protein fragment solution of the present invention comprises forming pieces of silk cocoons from the Bombyx mori silkworm; Extract the pieces at about 100°C in a solution of water and Na2CO3, for about 60 minutes, where the volume of water is about 0.4 times the weight of raw silk, and the amount of Na2CO3 is about 0.848 times the weight of the pieces to form an extract.

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Silk fibroin, rinse 3 marts of silk fibroin extract at about 60°C for about 20 minutes/rinse in a volume of rinse water, where the rinse water per cycle equals about 0.2 liters in weight of pieces; Removal of water increases the amount of silk fibroin extract. Drying silk fibroin extract; and dissolving the silk fibroin extract in a LiBr solution, whereby the LiBr solution is first heated to about 5 100°C to produce a LiBr solution and maintain it at this temperature; And apply the silk solution

and LiBr in a drying oven at about 100°C for about 60 minutes to obtain complete melting and further fragmentation of the original silk protein structure into a mixture of the desired molecular weight and polydispersity; Filtering the solution to remove any remaining silkworm residue; Dilute the solution with water to produce a 1% silk solution. The solvent is removed from the solution using tangential flow filtration (TFF). In an embodiment, 10 a 10 kDa membrane is used to purify the silk solution and produce the desired silk to water ratio

Final. TFF can then be used to further concentrate the pure silk solution to a 2% silk to water concentration.

Every process step from raw cocoons to membrane separation can be scaled to increase manufacturing efficiency. Whole cocoons are currently purchased as raw material, while pre-cleaned or non-heat-treated cocoons can also be used15, as deworming leaves minimal residue. Prepare cutting and cleaning cocoons

Manual operation. However, scaling up the process can be made less labor intensive, for example, by using an automatic machine in combination with compressed air to remove the worms and any particulate matter, or using a cutting mill to cut the cocoons into smaller pieces. The extraction step, which is currently performed in small batches, can be completed in a larger vessel, for example, an industrial washing machine where temperatures can be maintained at or between 60 and 100°C. The rinsing step can also be completed

In an industrial washing machine, dispense with the manual rinsing cycle. Dissolution of the silk solution in LiBr can occur in a vessel other than a convection furnace, for example a stirrer tank reactor. Membrane separation of silk through a series of water changes is a manual and time-consuming process, which can be accelerated by changing certain parameters, for example diluting the silk solution before

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Membrane separation. The membrane separation process can be scaled up for manufacturing using automated filtering equipment, for example, tangential flow filtration isotherms.

Changing the parameters of extraction (i.e., time and temperature), LiBr (i.e., the temperature of the LiBr solution when added to silk fibroin extract or vice versa) and solubilization (i.e., time and temperature) produce solutions

<p dir="rtl">5 Solvent and silk have different viscosities, consistencies, and colors (see Figures 5-32). Increasing the extraction temperature prolongs the extraction time, and results in using a higher temperature of the LiBr solution when immersing, more time when dissolving the silk, and increasing the time at the temperature (e.g. in oven as shown here, or an alternative heat source) All solvent and silk solutions are less viscous and more uniform. While most determinants produce a valuable silk solution, methods that allow complete dissolution are achieved in as little as 4-6 minutes.</p>

<p dir="rtl">10 Hours are preferred in order to enlarge the process.</p>

Figures (5-10) show photographic images of four different silk extraction combinations tested: at 90°C for 30 minutes, at 90°C for 60 minutes, at 100°C for 30 minutes, and at 100°C for 60 minutes. In short, a 9.3 LiBr molar solution was prepared and left Lye at room temperature for at least 30 minutes. 5 ml of LiBr solution was added to 1.25 g of silk and applied

<p dir="rtl">15 In an oven at 60°C. Samples from each group were taken out at 6.4, 8, 12, 24, 168, and 192 hours. The remaining sample was photographed.</p>

Figures (22-23) show photographic images of four different silk extraction combinations tested: at 90°C for 30 minutes, at 90°C for 60 minutes, at 100°C for 30 minutes, and at 100°C for 60 minutes. Briefly, a 9.3 M solution was heated to One of four temperatures: 60°C,

<p dir="rtl">20 80°C, 100°C, or boiling. 5 mL of heated LiBr solution was added to 1.25 g of silk</p>

And put them in an oven at 60°C. Samples from each group were taken out at 1, 4, and 6 hours. The remaining sample was photographed.

Figures (24-32) show photographic images of four different silk extraction combinations tested: at 90°C for 30 minutes, at 90°C for 60 minutes, at 100°C for 30 minutes, and at 100°C for

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<p dir="rtl">60 minute. Briefly, a 9.3 M solution was heated to one of four temperatures: 60°C, 80°C, 100°C, or boiling. 5 ml of hot LiBr solution was added to 1.25 g of silk and placed in an oven at the same temperature as the LiBr. Samples from each group were taken out at 1, 4, and 6 hours. 1 ml of each sample was added to 7.5 ml of 9.3 M LiBr and cooled for testing</p>

<p dir="rtl">5 Viscosity. The remaining sample was photographed.</p>

The molecular weight of silk protein fragments can be controlled based on the specific parameters used during the extraction step, including the time and temperature of extraction; The specific parameters used during the dissolution step include the temperature of the LiBr at the time of immersion of the fire in the lithium bromide and the time of immersion in the solution is maintained at specific temperatures, and the specific parameters 10 used during the filtration step. By controlling process parameters using the methods described

It is possible to produce mixture solutions with SPF polydispersity ≥ 2.5 when composed of different molecular weights ranging from 5 to 200 kDa, and most preferably between 10 and 80 kDa. By adjusting process parameters to obtain silk solutions of different molecular weights, a range of fragment mixture end products with polydispersity ≥ 2.5 can be targeted depending on pressure requirements.

<p dir="rtl">15 required. In addition, SPF mixture solutions with polydispersity &lt;</p>

2.5. Also, a mixture content of different medium molecular weights and dispersions can be mixed to produce solution combinations. Alternatively, liquid silk gland (100% sericin-free silk protein) removed directly from a worm can be used in combination with any of the SPF mixture solutions of the present invention. The molecular weight of the aqueous solution composition was determined from its basis protein fragments.

<p dir="rtl">20 Pure silk fibroin using high-pressure liquid chromatography (HPLC) with a refractive index detector (RID). Polydispersity was calculated using Cirrus GPC Online GPC/SEC software version 3.3 (Agilent).</p>

The parameters were changed while processing the raw silk cocoons into a silk solution. Changing these parameters affects the MW of the resulting silk solution. Treatment parameters include: (1) time and temperature

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Extraction, (2) LiBr solution temperature, (3) melting oven temperature, (4) dissolution time. The molecular weight was determined by the mass spectrum as shown in Figures (40-54).

Experiments were conducted to determine the effect of changing the extraction time. Figures (40-45) are graphs illustrating these results, and Tables (2-8) summarize the results. Below is the summary:

<p dir="rtl">5 A 30-minute sericin extraction time produced a greater MW than a 60-minute sericin extraction time.</p>

Say MW with oven residence time.

An oven and LiBr solution at 140°C produced a lower end of the confidence interval below a MW of 9500 daltons.

Extraction for 30 minutes at time points 1 and 4 hours produced undigested silk.

<p dir="rtl">10 The 30-minute extraction at the 1-hour time point gave a significantly high molecular weight with a lower end of the confidence interval of 35,000 Daltons.</p>

The MW range that reached the upper end of the confidence interval was 18,000-216,000 Daltons (important for providing solutions with a defined upper end).

<tr><td colspan="7"><p dir="rtl">Table 2: Effect of extraction time (30 minutes versus 60 minutes) on the molecular weight of silk processed under conditions of extraction temperature of 100°C, lithium bromide (LiBr) solution at 100°C, and melting oven at 100°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">Oven time</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td></tr><tr><td><p>1.63</p></td><td><p>93387</p></td><td><p>35093</p></td><td><p>12780</p></td><td><p>57247</p></td><td><p>1</p></td><td><p>30</p></td></tr><tr><td><p>2.71</p></td><td><p>85407</p></td><td><p>11633</p></td><td><p>1387</p></td><td><p>31520</p></td><td><p>1</p></td><td><p>60</p></td></tr><tr><td><p>2.87</p></td><td><p>117658</p></td><td><p>14268</p></td><td><p>2632</p></td><td><p>40973</p></td><td><p>4</p></td><td><p>30</p></td></tr>

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<tr><td><p>2.38</p></td><td><p>59803</p></td><td colspan="2"><p>10520</p></td><td colspan="2"><p>1248</p></td><td colspan="2"><p>25082</p></td><td colspan="2"><p>4</p></td><td><p>60</p></td></tr><tr><td><p>2.50</p></td><td><p>63943</p></td><td colspan="2"><p>10252</p></td><td colspan="2"><p>1405</p></td><td colspan="2"><p>25604</p></td><td colspan="2"><p>6</p></td><td><p>30</p></td></tr><tr><td><p>2.08</p></td><td><p>43695</p></td><td colspan="2"><p>10073</p></td><td colspan="2"><p>1262</p></td><td colspan="2"><p>20980</p></td><td colspan="2"><p>6</p></td><td><p>60</p></td></tr><tr><td colspan="11"><p dir="rtl">Table 3: Effect of extraction time (30 minutes versus 60 minutes) on the molecular weight of treated silk under conditions of extraction temperature of 100°C, boiling lithium bromide (LiBr) solution, and melting oven at 60°C for 4 hours</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="3"><p dir="rtl">Confidence interval</p></td><td colspan="2"><p dir="rtl">Standard curve</p></td><td colspan="2"><p>MW</p><p dir="rtl">Average</p></td><td colspan="2"><p dir="rtl">time</p><p dir="rtl">Boiling</p></td><td><p dir="rtl">the sample</p></td></tr><tr><td><p>2.87</p></td><td><p>142478</p></td><td colspan="2"><p>17306</p></td><td colspan="2"><p>4580</p></td><td colspan="2"><p>49656</p></td><td colspan="2"><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.69</p></td><td><p>80705</p></td><td colspan="2"><p>11183</p></td><td colspan="2"><p>1536</p></td><td colspan="2"><p>30042</p></td><td colspan="2"><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td colspan="11"><p dir="rtl">Table 4: Effect of extraction time (30 minutes versus 60 minutes) on the molecular weight of silk processed under conditions of extraction temperature of 100°C, lithium bromide (LiBr) solution at 100°C, and melting oven at 100°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td colspan="2"><p dir="rtl">Standard curve</p></td><td colspan="2"><p>MW</p><p dir="rtl">Average</p></td><td colspan="2"><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td><td><p dir="rtl">the sample</p></td></tr>

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<tr><td><p>2.63</p></td><td><p>153809</p></td><td><p>22201</p></td><td colspan="2"></td><td colspan="2"><p>58436</p></td><td colspan="2"><p>1</p></td><td><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">minute,</p><p dir="rtl">1</p><p dir="rtl">hour</p></td></tr><tr><td><p>2.66</p></td><td><p>84224</p></td><td><p>11931</p></td><td colspan="2"></td><td colspan="2"><p>31700</p></td><td colspan="2"><p>1</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">1</p><p dir="rtl">hour</p></td></tr><tr><td><p>2.89</p></td><td><p>178847</p></td><td><p>21463</p></td><td colspan="2"><p>13337</p></td><td colspan="2"><p>61956.5</p></td><td colspan="2"><p>4</p></td><td><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.56</p></td><td><p>65564</p></td><td><p>9979</p></td><td colspan="2"><p>2446</p></td><td colspan="2"><p>25578.5</p></td><td colspan="2"><p>4</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td colspan="11"><p dir="rtl">Table 5: Effect of extraction time (30 minutes versus 60 minutes) on the molecular weight of silk treated under conditions of extraction temperature of 100°C, lithium bromide solution at 80°C (LiBr) and melting oven at 80°C for 4 hours</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="3"><p dir="rtl">Confidence interval</p></td><td colspan="2"><p dir="rtl">Now I'm sorry</p></td><td colspan="2"><p>MW</p><p dir="rtl">Average</p></td><td colspan="2"><p dir="rtl">time</p><p dir="rtl">Boiling</p></td><td><p dir="rtl">the sample</p></td></tr>

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<tr><td><p>3.40</p></td><td><p>215775</p></td><td colspan="2"><p>18693</p></td><td colspan="2"></td><td colspan="2"><p>63510</p></td><td colspan="2"><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">Minute, 6</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.61</p></td><td><p>65706</p></td><td colspan="2"><p>9637</p></td><td colspan="2"><p>238</p></td><td colspan="2"><p>25164</p></td><td colspan="2"><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">Minute, 6</p><p dir="rtl">hours</p></td></tr><tr><td colspan="11"><p dir="rtl">Table 6: Effect of extraction time (30 minutes versus 60 minutes) on the molecular weight of silk processed under conditions of extraction temperature of 100°C, lithium bromide (LiBr) solution at 80°C, and melting oven at 60°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td colspan="2"><p dir="rtl">Standard curve</p></td><td colspan="2"><p>MW</p><p dir="rtl">Average</p></td><td colspan="2"><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td><td><p dir="rtl">the sample</p></td></tr><tr><td><p>3.10</p></td><td><p>183760</p></td><td><p>19073</p></td><td colspan="2"><p>14028</p></td><td colspan="2"><p>59202</p></td><td colspan="2"><p>4</p></td><td><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.56</p></td><td><p>67442</p></td><td><p>10266</p></td><td colspan="2"><p>637</p></td><td colspan="2"><p>26312.5</p></td><td colspan="2"><p>4</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.59</p></td><td><p>121293</p></td><td><p>18076</p></td><td colspan="2"></td><td colspan="2"><p>46824</p></td><td colspan="2"><p>6</p></td><td><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">minute,</p><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr>

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<tr><td><p>2.59</p></td><td><p>68302</p></td><td><p>10168</p></td><td></td><td><p>26353</p></td><td><p>6</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr><tr><td colspan="8"><p dir="rtl">Table 7: Effect of extraction time (30 minutes versus 60 minutes) on the molecular weight of silk processed under conditions of extraction temperature of 100°C, lithium bromide (LiBr) solution at 100°C, and melting oven at 100°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td><td><p dir="rtl">the sample</p></td></tr><tr><td><p>2.42</p></td><td><p>115900</p></td><td><p>19758</p></td><td></td><td><p>47853</p></td><td><p>4</p></td><td><p>30</p></td><td><p dir="rtl">30 minute,</p><p dir="rtl">4 hours</p></td></tr><tr><td><p>2.38</p></td><td><p>59804</p></td><td><p>10520</p></td><td><p>1248</p></td><td><p>25082</p></td><td><p>4</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.89</p></td><td><p>160366</p></td><td><p>19153</p></td><td><p>8992</p></td><td><p>55421</p></td><td><p>6</p></td><td><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">minute,</p><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.08</p></td><td><p>43694</p></td><td><p>10073</p></td><td><p>1262</p></td><td><p>20980</p></td><td><p>6</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p></td></tr>

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<tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr><tr><td colspan="8"><p dir="rtl">Table 8: Effect of extraction time (30 minutes versus 60 minutes) on the molecular weight of silk processed under conditions of extraction temperature of 100°C, lithium bromide (LiBr) solution at 100°C, and melting oven at 100°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td><td><p dir="rtl">the sample</p></td></tr><tr><td><p>2.00865</p></td><td><p>18127</p></td><td><p>4493</p></td><td><p>1102</p></td><td><p>9024.5</p></td><td><p>1</p></td><td><p>30</p></td><td><p dir="rtl">30 minute,</p><p dir="rtl">4 hours</p></td></tr><tr><td><p>2.2358</p></td><td><p>34762</p></td><td><p>6954</p></td><td></td><td><p>15548</p></td><td><p>1</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.1749</p></td><td><p>28318</p></td><td><p>5987</p></td><td></td><td><p>13021</p></td><td><p>4</p></td><td><p>30</p></td><td><p dir="rtl">30</p><p dir="rtl">minute,</p><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.0298</p></td><td><p>22100</p></td><td><p>5364</p></td><td></td><td><p>10888</p></td><td><p>4</p></td><td><p>60</p></td><td><p dir="rtl">60</p><p dir="rtl">minute,</p><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr>

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Experiments were conducted to determine the effect of changing the extraction temperature. Figure (47) is a graph illustrating these results, and Table (9) summarizes them. Below is the summary: Extraction of sericin at 90°C produced a higher MW than extraction at 100°C.

Both extraction at 90 and 100°C showed a decrease in MW with time in the oven.

<tr><td colspan="8"><p dir="rtl">Table 9: Effect of extraction temperature (90°C vs. 100°C) on the molecular weight of treated silk under conditions of 60-minute extraction time, lithium bromide (LiBr) solution at 100°C, and melting oven at 100°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td><td><p dir="rtl">the sample</p></td></tr><tr><td><p>2.79</p></td><td><p>104119</p></td><td><p>13368</p></td><td><p>4204</p></td><td><p>37308</p></td><td><p>4</p></td><td><p>30</p></td><td><p dir="rtl">90°C,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.38</p></td><td><p>59804</p></td><td><p>10520</p></td><td><p>1248</p></td><td><p>25082</p></td><td><p>4</p></td><td><p>60</p></td><td><p dir="rtl">100°C,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.69</p></td><td><p>92100</p></td><td><p>12717</p></td><td><p>1135</p></td><td><p>34224</p></td><td><p>6</p></td><td><p>30</p></td><td><p dir="rtl">90°C,</p><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.08</p></td><td><p>43694</p></td><td><p>10073</p></td><td><p>1261</p></td><td><p>20980</p></td><td><p>6</p></td><td><p>60</p></td><td><p dir="rtl">100°C,</p><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr>

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Experiments were conducted to determine the effect of changing the temperature of a lithium bromide (LiBr) solution when added to silk. Figures (48-49) are graphs that illustrate these results, and Tables (10-11) summarize them. Below is the summary.

No effect on MW or confidence interval (all confidence intervals were between 10,500 and 6,500 Daltons).

<p dir="rtl">5 almost,</p>

Studies have shown that, when LiBr is added and melted, the melting temperature of LiBr-silk quickly drops below the original LiBr temperature because most of the mass is silk at room temperature.

<tr><td colspan="8"><p dir="rtl">Table 10: Effect of lithium bromide (LiBr) temperature on the molecular weight of silk treated under the conditions, extraction time of 60 minutes, extraction temperature of 100°C, and melting oven at 60°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">Temperature degree</p><p>LiBr</p><p dir="rtl">)M(</p></td><td><p dir="rtl">the sample</p></td></tr><tr><td><p>2.66</p></td><td><p>84223</p></td><td><p>11931</p></td><td></td><td><p>31700</p></td><td><p>1</p></td><td><p>60</p></td><td><p dir="rtl">60°C,</p><p>,LiBr</p><p>1</p><p dir="rtl">hour</p></td></tr><tr><td><p>2.60</p></td><td><p>72552</p></td><td><p>10735</p></td><td><p>200</p></td><td><p>27907</p></td><td><p>1</p></td><td><p>100</p></td><td><p dir="rtl">100°C,</p><p>,LiBr</p><p>1</p><p dir="rtl">hour</p></td></tr>

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<tr><td><p>2.71</p></td><td><p>79119</p></td><td><p>10789</p></td><td><p>1082</p></td><td><p>29217</p></td><td><p>4</p></td><td><p>RT</p></td><td><p>RT</p><p>,LiBr</p><p>4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.56</p></td><td><p>65564</p></td><td><p>9978</p></td><td><p>2445</p></td><td><p>25578</p></td><td><p>4</p></td><td><p>60</p></td><td><p dir="rtl">60°C,</p><p>,LiBr</p><p>4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.56</p></td><td><p>67441</p></td><td><p>10265</p></td><td><p>637</p></td><td><p>26312</p></td><td><p>4</p></td><td><p>80</p></td><td><p dir="rtl">80°C,</p><p>,LiBr</p><p>4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.45</p></td><td><p>67931</p></td><td><p>11279</p></td><td><p>1729</p></td><td><p>27681</p></td><td><p>4</p></td><td><p>100</p></td><td><p dir="rtl">100°C,</p><p>,LiBr</p><p>4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.69</p></td><td><p>80704</p></td><td><p>11183</p></td><td><p>1535</p></td><td><p>30042</p></td><td><p>4</p></td><td><p>Boil</p></td><td><p>LiBr</p><p dir="rtl">boiling,</p><p dir="rtl">4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.46</p></td><td><p>65332</p></td><td><p>10783</p></td><td><p>1893</p></td><td><p>26543</p></td><td><p>6</p></td><td><p>RT</p></td><td><p>LiBr</p><p>,RT</p></td></tr>

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<tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p dir="rtl">6</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.59</p></td><td><p>68301</p></td><td><p>10167</p></td><td><p>916</p></td><td><p>26353</p></td><td><p>6</p></td><td><p>80</p></td><td><p dir="rtl">80°C,</p><p>,LiBr</p><p>6</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.46</p></td><td><p>66889</p></td><td><p>11020</p></td><td></td><td><p>27150</p></td><td><p>6</p></td><td><p>100</p></td><td><p dir="rtl">100°C,</p><p>,LiBr</p><p>6</p><p dir="rtl">hours</p></td></tr>

<tr><td colspan="8"><p dir="rtl">Table 11: Effect of lithium bromide (LiBr) temperature on the molecular weight of silk treated under the conditions, extraction time of 60 minutes, extraction temperature of 100°C, and melting oven at 60°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">Temperature degree</p><p>LiBr</p><p dir="rtl">)M(</p></td><td><p dir="rtl">the sample</p></td></tr><tr><td><p>2.89</p></td><td><p>178847</p></td><td><p>21463</p></td><td><p>13336</p></td><td><p>61956</p></td><td><p>4</p></td><td><p>60</p></td><td><p dir="rtl">60°C,</p><p>,LiBr</p><p>4</p><p dir="rtl">hours</p></td></tr>

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<tr><td><p>3.10</p></td><td><p>183760</p></td><td><p>19073</p></td><td><p>14027</p></td><td><p>59202</p></td><td><p>4</p></td><td><p>80</p></td><td><p dir="rtl">80m,</p><p>,LiBr</p><p>4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.42</p></td><td><p>115899</p></td><td><p>19757</p></td><td></td><td><p>47853</p></td><td><p>4</p></td><td><p>100</p></td><td><p dir="rtl">100°C,</p><p>,LiBr</p><p>4</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.59</p></td><td><p>121292</p></td><td><p>18075</p></td><td></td><td><p>46824</p></td><td><p>6</p></td><td><p>80</p></td><td><p dir="rtl">80°C,</p><p>,LiBr</p><p>6</p><p dir="rtl">hours</p></td></tr><tr><td><p>2.89</p></td><td><p>160366</p></td><td><p>19152</p></td><td><p>8991</p></td><td><p>55421</p></td><td><p>6</p></td><td><p>100</p></td><td><p dir="rtl">100°C,</p><p>,LiBr</p><p>6</p><p dir="rtl">hours</p></td></tr>

Experiments were conducted to determine the effect of changing the oven/melting temperature. Figures (50-54) are drawings

Graphics illustrate these results, and tables (12-156) summarize them. Below is the summary:

Oven temperature had a lesser effect on silk extracted at 60 minutes than silk extracted at 60 minutes.

30 minute. Without wanting to adhere to a theory, it is believed that silk extracted for 30 minutes is...

5 Less dissolution during extraction and hence oven temperature has a greater influence on the larger MW, less dissolvable fraction of silk.

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For an oven at 60°C versus 140°C, silk extracted for 30 minutes showed a very significant effect in reducing MW at a higher oven temperature, while silk extracted for 60 minutes had a much smaller effect.

An oven at 140°C produced a lower end of the confidence interval at approximately 6000 daltons.

<tr><td colspan="8"><p dir="rtl">Table 12: Effect of oven/melting temperature on the molecular weight of silk processed under conditions of extraction temperature of 100°C, extraction time of 30 minutes, and lithium bromide (LiBr) solution at 100°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">Oven temperature (°C)</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td></tr><tr><td><p>2.42</p></td><td><p>115900</p></td><td><p>19758</p></td><td></td><td><p>47853</p></td><td><p>4</p></td><td><p>60</p></td><td><p>30</p></td></tr><tr><td><p>2.87</p></td><td><p>117658</p></td><td><p>14268</p></td><td><p>2632</p></td><td><p>40973</p></td><td><p>4</p></td><td><p>100</p></td><td><p>30</p></td></tr><tr><td><p>2.89</p></td><td><p>160366</p></td><td><p>19153</p></td><td><p>8992</p></td><td><p>55421</p></td><td><p>6</p></td><td><p>60</p></td><td><p>30</p></td></tr><tr><td><p>2.50</p></td><td><p>63943</p></td><td><p>10252</p></td><td><p>1405</p></td><td><p>25604</p></td><td><p>6</p></td><td><p>100</p></td><td><p>30</p></td></tr><tr><td colspan="8"><p dir="rtl">Table 13: Effect of oven/melting temperature on the molecular weight of silk processed under conditions of extraction temperature of 100°C, extraction time of 60 minutes, and lithium bromide (LiBr) solution at 100°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">Oven temperature (°C)</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td></tr>

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<tr><td><p>2.60</p></td><td><p>72552</p></td><td><p>10735</p></td><td><p>200</p></td><td><p>27908</p></td><td><p>1</p></td><td><p>60</p></td><td><p>60</p></td></tr><tr><td><p>2.71</p></td><td><p>85407</p></td><td><p>11633</p></td><td><p>1387</p></td><td><p>31520</p></td><td><p>1</p></td><td><p>100</p></td><td><p>60</p></td></tr><tr><td><p>2.62</p></td><td><p>72552</p></td><td><p>11279</p></td><td><p>1730</p></td><td><p>27681</p></td><td><p>4</p></td><td><p>60</p></td><td><p>60</p></td></tr><tr><td><p>2.38</p></td><td><p>59803</p></td><td><p>10520</p></td><td><p>1248</p></td><td><p>25082</p></td><td><p>4</p></td><td><p>100</p></td><td><p>60</p></td></tr><tr><td><p>2.46</p></td><td><p>66889</p></td><td><p>11020</p></td><td><p>916</p></td><td><p>27150</p></td><td><p>6</p></td><td><p>60</p></td><td><p>60</p></td></tr><tr><td><p>2.08</p></td><td><p>43695</p></td><td><p>10073</p></td><td><p>1262</p></td><td><p>20980</p></td><td><p>6</p></td><td><p>100</p></td><td><p>60</p></td></tr><tr><td colspan="8"><p dir="rtl">Table 14: Effect of oven/melting temperature on the molecular weight of silk processed under conditions of extraction temperature of 100°C, extraction time of 60 minutes, and lithium bromide (LiBr) solution at 140°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">Oven temperature (°C)</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td></tr><tr><td><p>2.69</p></td><td><p>80705</p></td><td><p>11183</p></td><td><p>1536</p></td><td><p>30042</p></td><td><p>4</p></td><td><p>60</p></td><td><p>60</p></td></tr><tr><td><p>2.14</p></td><td><p>33322</p></td><td><p>7255</p></td><td></td><td><p>15548</p></td><td><p>4</p></td><td><p>140</p></td><td><p>60</p></td></tr><tr><td colspan="8"><p dir="rtl">Table 15: Effect of oven/melting temperature on the molecular weight of silk processed under conditions of extraction temperature of 100°C, extraction time of 30 minutes, and lithium bromide (LiBr) solution at 140°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">Temperature degree</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td></tr>

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<tr><td></td><td colspan="2"></td><td></td><td></td><td></td><td><p dir="rtl">Oven (m)</p></td><td></td></tr><tr><td><p>2.87</p></td><td><p>142478</p></td><td><p>17306</p></td><td><p>4580</p></td><td><p>49656</p></td><td><p>4</p></td><td><p>60</p></td><td><p>30</p></td></tr><tr><td><p>2.01</p></td><td><p>18127</p></td><td><p>4493</p></td><td><p>1102</p></td><td><p>9025</p></td><td><p>4</p></td><td><p>140</p></td><td><p>30</p></td></tr><tr><td><p>3.37</p></td><td><p>199889</p></td><td><p>17641</p></td><td><p>11640</p></td><td><p>59383</p></td><td><p>6</p></td><td><p>60</p></td><td><p>30</p></td></tr><tr><td><p>2.17</p></td><td><p>28319</p></td><td><p>5987</p></td><td></td><td><p>13021</p></td><td><p>6</p></td><td><p>140</p></td><td><p>30</p></td></tr><tr><td colspan="8"><p dir="rtl">Table 16: Effect of oven/melting temperature on the molecular weight of silk processed under conditions of extraction temperature of 100°C, extraction time of 60 minutes, and lithium bromide (LiBr) solution at 80°C (oven/melting time was variable)</p></td></tr><tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p></td><td colspan="2"><p dir="rtl">Confidence interval</p></td><td><p dir="rtl">Standard curve</p></td><td><p>MW</p><p dir="rtl">Average</p></td><td><p dir="rtl">time</p><p dir="rtl">the oven</p></td><td><p dir="rtl">Oven temperature (°C)</p></td><td><p dir="rtl">time</p><p dir="rtl">Boiling</p></td></tr><tr><td><p>2.56</p></td><td><p>67442</p></td><td><p>10266</p></td><td><p>637</p></td><td><p>26313</p></td><td><p>4</p></td><td><p>60</p></td><td><p>60</p></td></tr><tr><td><p>2.47</p></td><td><p>74806</p></td><td><p>12279</p></td><td><p>4293</p></td><td><p>30308</p></td><td><p>4</p></td><td><p>80</p></td><td><p>60</p></td></tr><tr><td><p>2.59</p></td><td><p>68302</p></td><td><p>10168</p></td><td></td><td><p>26353</p></td><td><p>6</p></td><td><p>60</p></td><td><p>60</p></td></tr><tr><td><p>2.61</p></td><td><p>65706</p></td><td><p>9637</p></td><td><p>238</p></td><td><p>25164</p></td><td><p>6</p></td><td><p>80</p></td><td><p>60</p></td></tr>

In one embodiment, when producing silk gel, an acid is used to help facilitate gel formation. In an embodiment, when producing a silk gel that includes a neutral or basic therapeutic molecule and/or agent, an acid may be added to facilitate gel formation. In one embodiment, when producing a silk gel, increasing the pH increases the storage stability of the gel. In an example, when producing silk gel, the number increases

<p dir="rtl">5 Hydrogenation (making the gel more basic, allows a greater amount of a basic molecule to be loaded into the gel.</p>

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In an embodiment, natural additives may be added to the silk gel to further stabilize the additives. For example, trace elements selenium, magnesium, or L-methionine can be used. Light-blocking contents can also be added to further increase stability.

In an embodiment, the methods disclosed herein produce a solution with controllable properties

<p dir="rtl">5 During manufacturing, including but not limited to: MW - can be changed by changing extraction and/or dissolution time, temperature (e.g. LiBr temperature), pressure, and filtration (size exclusion chromatography, for example) Structure - E Removal or cleavage of the light or heavy chain of the fibroin protein polymer; purification by controlling the temperature of the hot rinse water to improve the removal of sericin or the filtration capacity to improve the removal of particulate matter that adversely affects the storage stability of the solution.</p>

<p dir="rtl">10 Silk fragment protein mixture; Color - the color of the solution can be controlled, for example, by the LiBr temperature and time; Viscosity, clarity, and stability of the solution. The resulting pH of the solution is usually around 7 and can be adjusted with an acid or base as appropriate for storage requirements.</p>

In an embodiment, the aforementioned SPF mixture solutions can be used to coat at least a portion of a fabric that can be used to produce textiles. In an embodiment, the aforementioned SPF mixture solutions 15 can be spun into yarn that can be used as fabric in textiles.

Figure (33) shows 2 HPLC chromatograms of samples containing vitamin C. The HPLC chromatograms show peaks from (1) a sample of chemically stabilized vitamin C at atmospheric conditions and (2) a sample of vitamin C taken after 1 hour at atmospheric conditions without fixation. Chemical to prevent oxidation, where the degradation products are visible. Figure (36): Table summarizing the stability of vitamin C in chemically stabilized solutions 20.

In some embodiments, a composition of the present invention may also include a skin penetration enhancer, including, but not limited to, sulfoxide compounds (such as dimethyl sulfoxide), pyrrolidone compounds (such as 2-pyrrolidone), alcohols (such as ethanol or decanol), azone compounds (such as lorocaparm and 1-dodecyl az-cycloheptane-2-one), surfactants (including alkyl carboxylates, and their acids

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Analogues such as oleic acid, fluoroalkyl carboxylates and their corresponding acids, alkyl sulfate compounds, alkyl ether sulfate compounds, docoazate compounds such as sodium dioctyl sulfosuccinate, alkyl benzene sulfonate compounds, alkyl ether phosphate compounds, and alkyl aryl ether phosphate compounds), glycol compounds (such as propylene glycol), terpene compounds (such as limonene, p-cymene, g-arneol,

5 Faresol, eugenol, fentol, terpenol, carveol, carvone, vincone, and verbenone), and isosorbide dimethyl.

The following are non-restrictive examples of suitable ranges for various determinants in silk solutions and for their preparation of the present invention. The silk solutions of the present invention may include one or more but not all of these determinants, and may be prepared using various combinations of the ranges of those determinants.

<p dir="rtl">10 In one embodiment, the percentage of silk in the solution is less than 30%. In one embodiment, the percentage of silk in the solution is less than 25%. In one embodiment, the percentage of silk in the solution is less than 20%. In one embodiment, the percentage of silk in the solution is less than 19%. In one embodiment, the percentage of silk in the solution is less than 18%. In one embodiment, the percentage of silk in the solution is less than 17%. In an embodiment, the percentage of silk in</p>

<p dir="rtl">15 The solution is less than 16%. In one embodiment, the percentage of silk in the solution is less than 15%. In one embodiment, the percentage of silk in the solution is less than 14%. In one embodiment, the percentage of silk in the solution is less than 13%. In one embodiment, the percentage of silk in the solution is less than 12%. In one embodiment, the percentage of silk in the solution is less than 11%. In one embodiment, the percentage of silk in the solution is less than 10%. In some form, it is</p>

<p dir="rtl">20 The percentage of silk in the solution is less than 9%. In one embodiment, the percentage of silk in the solution is less than 8%. In one embodiment, the percentage of silk in the solution is less than 7%. In one embodiment, the percentage of silk in the solution is less than 6%. In one embodiment, the percentage of silk in the solution is less than 5%. In one embodiment, the percentage of silk in the solution is less than 4%. In one embodiment, the percentage of silk in the solution is less than 3%. In a model</p>

<p dir="rtl">25 However, the percentage of silk in the solution is less than 1%. In a model, it is a percentage</p>

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The silk content in the solution is less than 0.9%. In one embodiment, the percentage of silk in the solution is lower

From 0.8%. In one embodiment, the percentage of silk in the solution is less than 0.7%. In a model

However, the percentage of silk in the solution is less than 0.6%. In a model, it is a percentage

For silk in solution less than 0.5%. In one embodiment, the percentage of silk in the solution is lower

<p dir="rtl">5 From 0.4%. In one embodiment, the percentage of silk in the solution is less than 0.3%. In one embodiment, the percentage of silk in the solution is less than 0.2%. In one embodiment, the percentage of silk in the solution is less than 0.1%. In one embodiment, the percentage of silk in the solution is greater than 0.1%. In one embodiment, the percentage of silk in the solution is greater than 0.2%. In one embodiment, the percentage of silk in the solution is greater than 0.3%. In a model, it is a percentage</p>

<p dir="rtl">10 For silk in solution greater than 0.4%. In one embodiment, the percentage of silk in the solution is greater than 0.5%. In one embodiment, the percentage of silk in the solution is greater than 0.6%. In one embodiment, the percentage of silk in the solution is greater than 0.7%. In one embodiment, the percentage of silk in the solution is greater than 0.8%. In one embodiment, the percentage of silk in the solution is greater than 0.9%. In an embodiment, the percentage of silk in the solution is greater than 1%.</p>

<p dir="rtl">15 In one embodiment, the percentage of silk in the solution is greater than 2%. In one embodiment, the percentage of silk in the solution is greater than 3%. In one embodiment, the percentage of silk in the solution is greater than 4%. In one embodiment, the percentage of silk in the solution is greater than 5%. In one embodiment, the percentage of silk in the solution is greater than 6%. In one embodiment, the percentage of silk in the solution is greater than 7%. In one embodiment, the percentage of silk in the solution is greater</p>

<p dir="rtl">20 From 8%. In one embodiment, the percentage of silk in the solution is greater than 9%. In one embodiment, the percentage of silk in the solution is greater than 10%. In one embodiment, the percentage of silk in the solution is greater than 11%. In one embodiment, the percentage of silk in the solution is greater than 12%. In one embodiment, the percentage of silk in the solution is greater than 13%. In one embodiment, the percentage of silk in the solution is greater than 14%. In a model, it is a percentage</p>

<p dir="rtl">25 For silk in solution greater than 15%. In one embodiment, the percentage of silk in the solution is greater than 16%. In one embodiment, the percentage of silk in the solution is greater than 17%. In a model</p>

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Usually, the percentage of silk in the solution is greater than 18%. In one embodiment, the percentage of silk in the solution is greater than 19%. In one embodiment, the percentage of silk in the solution is greater than 20%. In one embodiment, the percentage of silk in the solution is greater than 25%. In one embodiment, the percentage of silk in the solution ranges from 0.1 to 25%. In one model, the ratio varies

<p dir="rtl">5 The percentage of silk in the solution is between 0.1 and 20%. In one embodiment, the percentage of silk in the solution is between 0.1 and 15%. In one embodiment, the percentage of silk in the solution is between 0.1 and 10%. In one embodiment, the percentage of silk in the solution is between 0.1 and 9%. In one embodiment, the percentage of silk in the solution is between 0.1 and 8%. In one embodiment, the percentage of silk in the solution is between 0.1 and 7%. In an embodiment, the percentage of silk in the solution ranges between...</p>

<p dir="rtl">10 0.1 and 6.5%. In one embodiment, the percentage of silk in the solution is between 0.1 and 6%. in</p>

Typically, the percentage of silk in the solution ranges between 0.1 and 5.5%. In one embodiment, the percentage of silk in the solution is between 0.1 and 5%. In one embodiment, the percentage of silk in the solution is between 0.1 and 4.5%. In one embodiment, the percentage of silk in the solution is between 0.1 and 4%. In one embodiment, the percentage of silk in the solution is between 0.1 and 3.5%. In an example,

<p dir="rtl">15 The percentage of silk in the solution ranges between 0.1 and 3%. In a model, the percentage varies</p>

For silk in solution between 0.1 and 2.5%. In one embodiment, the percentage of silk in the solution is between 0.1 and 2.0%. In one embodiment, the percentage of silk in the solution is between 0.1 and 2.4%. In one embodiment, the percentage of silk in the solution is between 0.5 and 5%. In one embodiment, the percentage of silk in the solution is between 0.5 and 4%. In an embodiment, the percentage of silk varies

<p dir="rtl">20 In solution between 0.5 and 3.5%. In one embodiment, the percentage of silk in the solution is between 0.5 and 3%. In one embodiment, the percentage of silk in the solution is between 0.5 and 2.5%. In one embodiment, the percentage of silk in the solution is between 1 and 4%. In one embodiment, the percentage of silk in the solution is between 1 and 3.5%. In one embodiment, the percentage of silk in the solution is between 1 and 3%. In one embodiment, the percentage of silk in the solution is between 1 and 2.5%. In an example,</p>

<p dir="rtl">25 The percentage of silk in the solution ranges between 1 and 2.4%. In a model, the percentage varies</p>

For silk in solution between 1 and 2%. In an embodiment, the percentage of silk in the solution ranges between...

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<p dir="rtl">20 And 30%. In one embodiment, the percentage of silk in the solution is between 0.1 and 6%. In one embodiment, the percentage of silk in the solution is between 6 and 10%. In one embodiment, the percentage of silk in the solution is between 6 and 8%. In one embodiment, the percentage of silk in the solution is between 16 and 9%. In one embodiment, the percentage of silk in the solution is between 10 and 20%. In a model</p>

<p dir="rtl">5 However, the percentage of silk in the solution ranges between 11 and 19%. In one embodiment, the percentage of silk in the solution is between 12 and 18%. In one embodiment, the percentage of silk in the solution is between 13 and 17%. In one embodiment, the percentage of silk in the solution is between 14 and 16%. In one embodiment, the percentage of silk in the solution is 2.4%. In one embodiment, the percentage of silk in the solution ranges from 2.0.</p>

<p dir="rtl">10 In one embodiment, the percentage of sericin in solution ranges from undetectable to 30%. In one embodiment, the percentage of sericin in solution ranges from undetectable to 5%. In one embodiment, the percentage of sericin in the solution is 1%. In one embodiment, the percentage of sericin in the solution is 2%. In one embodiment, the percentage of sericin in the solution is 3%. In one embodiment, the percentage of sericin in the solution is 4%. In a model, it is a percentage</p>

<p dir="rtl">15 Sericin in 5% solution. In one embodiment, the percentage of sericin in the solution is 10%. In one embodiment, the percentage of sericin in the solution is 30%.</p>

In one embodiment, the stability of the LiBr solution varies between 0 and 1 year. In one embodiment, the stability of the LiBr solution varies between 0 and 2 years. In one embodiment, the stability of the LiBr solution varies between 0 and 3 years. In one embodiment, the stability of the LiBr solution varies between 0 and 4 years. In an embodiment, the stability of the LiBr solution varies between...

<p dir="rtl">20 Zero and 5 years old. In one embodiment, the stability of the LiBr solution is between 1 and 2 years. In one embodiment, the stability of the LiBr solution varies between 1 and 3 years. In one embodiment, the stability of the LiBr solution varies between 1 and 4 years. In one embodiment, the stability of the LiBr solution varies between 1 and 5 years. In one embodiment, the stability of the LiBr solution is between 2 and 3 years. In one embodiment, the stability of the LiBr solution varies between 2 and 4 years. In one embodiment, the stability of the LiBr solution ranges from 2 to 5 years. In one embodiment, the stability of the LiBr solution is between 3 and 4 years. in</p>

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Typically, the stability of the LiBr solution ranges between 3 and 5 years. In one embodiment, the stability of the LiBr solution is between 4 and 5 years.

In an embodiment, the stability of the composition of the present invention varies between 10 days and 6 months. In an embodiment, the stability of the composition of the present invention is between 6 and 12 months. In one embodiment, the composition of the present invention is constant

<p dir="rtl">5 Between 12 and 18 months. In one embodiment, the stability of the composition of the present invention is between 18 and 24 months. In an embodiment, the stability of the composition of the present invention is between 24 and 30 months. In an embodiment, the stability of the composition of the present invention is between 30 and 36 months. In an embodiment, the stability of the composition of the present invention is between 36 and 48 months. In an embodiment, the stability of the composition of the present invention is between 48 and 60 months.</p>

In one embodiment, a composition of the present invention comprises an aqueous solution of protein based fragments

<p dir="rtl">10 Pure silk fibroin has an average molecular weight that ranges between 6 and 16 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight ranging from 17 to 38 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight ranging from 39 to 80 kDa. In one embodiment, a composition of the present invention includes:</p>

<p dir="rtl">15 An aqueous solution of protein fragments based on pure silk fibroin has an average molecular weight ranging between 1 and 5 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 5 to 10 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of between 10 and 15 kDa. in</p>

<p dir="rtl">20 In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 15 to 20 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 20 to 25 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight</p>

<p dir="rtl">25 My weight ranges between 25 and 30 kilodaltons. In one embodiment, a composition of the present invention includes:</p>

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An aqueous solution of protein fragments based on pure silk fibroin has an average molecular weight ranging between 30 and 35 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 35 to 40 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments

<p dir="rtl">5 It is based on pure silk fibroin and has an average molecular weight that ranges between 40 and 45 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 45 to 50 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 50 to 55 kDa. In an embodiment, a composition of the invention includes</p>

<p dir="rtl">10 The current analysis of aqueous solutions consists of protein fragments based on pure silk fibroin, with an average molecular weight that ranges between 55 and 60 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 60 to 65 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of between 65 and 70 kilograms.</p>

<p dir="rtl">15 Dalton. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 70 to 75 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 75 to 80 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight</p>

<p dir="rtl">20 Its average molecular weight ranges between 80 and 85 kilodaltons. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 85 to 90 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 90 to 95 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of</p>

<p dir="rtl">25 Protein fragments based on pure silk fibroin have an average molecular weight ranging between 95 and 100 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments</p>

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It is based on pure silk fibroin and has an average molecular weight that ranges between 100 and 105 kilodaltons. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 105 to 110 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments based on pure silk fibroin

<p dir="rtl">5 It has an average molecular weight that ranges between 110 and 115 kilodaltons. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 115 to 120 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 120 to 125 kDa. In one embodiment, a composition of the present invention includes a solution</p>

<p dir="rtl">10 Hydrolyzed protein fragments based on pure silk fibroin have an average molecular weight that ranges between 125 and 130 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 130 to 135 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 135 to 140 kDa. in</p>

<p dir="rtl">15 In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 140 to 145 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 145 to 150 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a molecular weight</p>

<p dir="rtl">20 My average weight ranges between 150 and 155 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 155 to 160 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 160 to 165 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of fragments</p>

<p dir="rtl">25 A protein based on pure silk fibroin. It has an average molecular weight that ranges between 165 and 170 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments</p>

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It is based on pure silk fibroin and has an average molecular weight that ranges between 170 and 175 kilodaltons. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 175 to 180 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments based on pure silk fibroin

<p dir="rtl">5 It has an average molecular weight that ranges between 180 and 185 kilodaltons. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 185 to 190 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 195 to 200 kDa. In one embodiment, a composition of the present invention includes a solution</p>

<p dir="rtl">10 Hydrolyzed protein fragments based on pure silk fibroin have an average molecular weight that ranges between 200 and 205 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 205 to 2105 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 210 to 215 kDa. in</p>

<p dir="rtl">15 In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 215 to 220 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 220 to 225 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a molecular weight</p>

<p dir="rtl">20 My average weight ranges between 225 and 230 kilodaltons. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 2300 to 235 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 235 to 240 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of fragments</p>

<p dir="rtl">25 A protein based on pure silk fibroin. It has an average molecular weight that ranges between 240 and 245 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments</p>

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It is based on pure silk fibroin and has an average molecular weight that ranges between 245 and 250 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 250 to 255 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments based on pure silk fibroin

<p dir="rtl">5 It has an average molecular weight that ranges between 255 and 260 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 260 to 265 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 265 to 270 kDa. In one embodiment, a composition of the present invention includes a solution</p>

<p dir="rtl">10 Hydrolyzed protein fragments based on pure silk fibroin have an average molecular weight that ranges between 270 and 275 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 275 to 280 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 285 to 290 kDa. in</p>

<p dir="rtl">15 In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of 290 to 295 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of between 295 and 300 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a molecular weight</p>

<p dir="rtl">20 My average weight ranges between 300 and 305 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 305 to 310 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 310 to 315 kDa. In an embodiment, a composition of the present invention comprises an aqueous solution of fragments</p>

<p dir="rtl">25 A protein based on pure silk fibroin. It has an average molecular weight that ranges between 315 and 320 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments</p>

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It is based on pure silk fibroin and has an average molecular weight that ranges between 320 and 325 kilodaltons. In an embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 325 to 330 kDa. In one embodiment, a composition of the present invention comprises an aqueous solution of protein fragments based on pure silk fibroin

<p dir="rtl">5 It has an average molecular weight that ranges between 330 and 335 kilodaltons. In an embodiment, the combination includes:</p>

The present invention uses an aqueous solution of protein fragments based on pure silk fibroin with an average molecular weight that ranges between 335 and 340 kilodaltons. In one embodiment, a composition of the present invention comprises an aqueous solution of pure silk fibroin-based protein fragments having a weight-average molecular weight of 340 to 345 kDa. In one embodiment, a composition of the present invention includes a solution

<p dir="rtl">10 Hydrolyzed protein fragments based on pure silk fibroin have an average molecular weight that ranges between 345 and 350 kilodaltons.</p>

In an embodiment, the composition of the present invention having an aqueous solution of pure silk fibroin-based protein fragments has a polydispersity ranging from about 1 to 5.0. In an embodiment, the composition of the present invention in which the aqueous solution of pure silk fibroin-based protein fragments has a polydispersity of

<p dir="rtl">15 Between about 1.5 and 3.0. In one embodiment, the composition of the present invention having an aqueous solution of pure silk fibroin-based protein fragments has a polydispersity of about 1 to 1.5. In one embodiment, the composition of the present invention having an aqueous solution of pure silk fibroin-based protein fragments has a polydispersity of about 1.5 to 2.0. In one embodiment, the composition of the present invention in which the aqueous solution of pure silk fibroin-based protein fragments has a polydispersity of about</p>

<p dir="rtl">20 2.0 and 2.5. In an embodiment, the composition of the present invention having an aqueous solution of fragments</p>

Protein based pure silk fibroin polydispersity ranges between about 2.0 and 3.0. In an embodiment, the composition of the present invention having an aqueous solution of pure silk fibroin-based protein fragments has a polydispersity of about 2.5 to 3.0.

In one embodiment, the composition of the present invention having an aqueous solution of protein fragments as its basis

<p dir="rtl">25 Pure silk fibroin undetectable levels of LiBr residues. In some form, it goes</p>

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The amount of LiBr residues in the composition of the present invention is between 10 and 1000 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention ranges from 10 to 300 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 25 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 50

<p dir="rtl">5 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 75 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 100 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 200 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 300 ppm. In an embodiment, a quantity of building blocks</p>

<p dir="rtl">10 LiBr in the composition of the present invention is less than 400 ppm. In an embodiment, the amount of LiBr residues in the composition of the present invention is less than 500 ppm. In an embodiment, the amount of LiBr residues in the composition of the present invention is less than 600 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 600 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 700</p>

<p dir="rtl">15 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 800 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is less than 900 ppm. In one embodiment the amount of LiBr residues in the composition of the present invention ranges between a non-detectable level and 500 ppm. In one embodiment the amount of LiBr residues in the composition of the present invention ranges between a non-detectable level and 450 parts per</p>

<p dir="rtl">20 Million. In one embodiment the amount of LiBr residues in the composition of the present invention ranges between a non-detectable level and 400 ppm. In one embodiment the amount of LiBr residues in the composition of the present invention ranges between a non-detectable level and 350 ppm. In one embodiment the amount of LiBr residues in the composition of the present invention ranges between a non-detectable level and 300 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention ranges between a level of no</p>

<p dir="rtl">25 It can be detected at 250 ppm. In one embodiment the amount of LiBr residues in the composition of the present invention ranges between a non-detectable level and 200 ppm. In some cases, the quantity varies</p>

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LiBr residues in the composition of the present invention are between an undetectable level and 150 ppm. In one embodiment the amount of LiBr residues in the composition of the present invention ranges between a non-detectable level and 100 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is between 100 and 200 ppm. In one embodiment, the amount of LiBr 5 residues in the composition of the present invention is between 200 and 300 ppm. In an embodiment, the quantity ranges from units

The composition of LiBr in the composition of the present invention is between 300 and 400 ppm. In one embodiment, the amount of LiBr residues in the composition of the present invention is between 400 and 500 ppm.

In one embodiment, the composition of the present invention has an aqueous solution based on protein fragments

Pure silk fibroin has undetectable levels of Na2CO3 residues. In one embodiment, 10 the amount of Na2CO3 residues in the composition of the invention is reduced to less than 100 ppm. in

In one embodiment, the amount of Na2CO3 residues in the composition of the invention is less than 200 parts per million. In one embodiment, the amount of Na2CO3 residues in the composition of the invention is less than 300 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the invention is less than 400 ppm. In one embodiment, the amount of Na2CO3 residues in 15 of the inventor's compositions is less than 500 ppm. In an embodiment, a quantity of building blocks

Na2CO3 in the test formula was reduced to less than 600 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the invention is less than 700 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the invention is less than 800 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the invention is less than 900 parts per

<p dir="rtl">20 Million. In one embodiment, the amount of Na2CO3 residues in the composition of the invention is less than</p>

1000 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention ranges from undetectable to 500 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention ranges from undetectable to 450 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention ranges from undetectable

<p dir="rtl">25 And 400 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the invention varies</p>

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The current is between undetectable and 350 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention ranges from undetectable to 300 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention ranges from undetectable to 250 ppm. In an embodiment, the amount of Na2CO3 residues in the composition of the present invention 5 ranges from undetectable to 200 ppm. In an embodiment, the quantity of building blocks varies

Na2CO3 in the present invention composition is between undetectable and 150 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention ranges from undetectable to 100 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention is between 100 and 200 ppm. In one embodiment, the amount of Na2CO3 10 residues in the composition of the present invention is between 200 and 300 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention is between 300 and 400 ppm. In one embodiment, the amount of Na2CO3 residues in the composition of the present invention is between 400 and 500 ppm.

In one embodiment, the water solubility of the aqueous solution of pure silk fibroin 15-based protein fragments of the present invention ranges from 50 to 100%. In one embodiment, the solubility varies in water

The aqueous solution of protein fragments based on pure silk fibroin of the present invention is between 60 and 100%. In one embodiment, the water solubility of the aqueous solution of pure silk fibroin-based protein fragments of the present invention ranges from 70 to 100%. In one embodiment, the water solubility of the aqueous solution of pure silk fibroin-based protein fragments of the present invention ranges from 80 to 100%. 20 In one embodiment, the water solubility of the aqueous solution of pure silk fibroin-based protein fragments of the present invention ranges between 90 and 100%. In one embodiment, the aqueous solution is composed of pure silk fibroin-based protein fragments of the present invention that do not dissolve in aqueous solutions.

In one embodiment, the solubility of the aqueous solution of pure silk fibroin-based protein fragments of the present invention in organic solutions ranges between 50 and 100%. In one embodiment, the solubility of the aqueous solution of pure silk fibroin-based protein fragments of the present invention ranges 25 in organic solutions

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Between 60 and 100%. In one embodiment, the solubility of the aqueous solution of pure silk fibroin-based protein fragments of the present invention in organic solutions ranges between 70 and 100%. In one embodiment, the solubility of the aqueous solution of pure silk fibroin-based protein fragments of the present invention in organic solutions ranges between 80 and 100%. In one embodiment, the solubility of the aqueous solution of pure silk fibroin-based protein 5 fragments of the present invention in organic solutions ranges between 90 and 100%. in

Typically, the aqueous solution consists of protein fragments based on pure silk fibroin, which are insoluble in organic solutions.

In one embodiment, the extraction temperature during the method of preparing the composition of the present invention is greater than 84°C. In one embodiment, the extraction temperature during the method of preparing the composition of the invention

<p dir="rtl">10 Current, less than 100°C. In one embodiment, the extraction temperature during the method of preparing the composition of the present invention ranges between 84 and 100°C. In one embodiment, the extraction temperature during the method of preparing the composition of the present invention ranges between 84 and 94°C. In one embodiment, the extraction temperature during the method of preparing the composition of the present invention ranges between 94 and 100°C.</p>

The following examples are provided to provide those of ordinary skill in the art with a complete description of how

<p dir="rtl">15 The making and use of said embodiments are neither intended to limit the scope of the inventors' intended use of their invention nor are they intended to constitute the only or all subsequent experiments to be performed. Efforts have been made to ensure accuracy with regard to the numbers used (e.g., quantities, temperature, etc.), but some experimental errors and deviations must be taken into account. Unless otherwise stated, parts are considered to be parts by weight, and that weight Molecular is a molecular weight</p>

<p dir="rtl">20 My average weight, temperature is in degrees Celsius, and pressure is atmospheric or close to it.</p>

Disclosed are textiles that are at least partially surface treated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention such that a silk coating is produced on the textile. In an embodiment, the silk coating of the present invention is available in the form of a spray liquid that can be sprayed onto textiles by a consumer. In an embodiment, textiles comprising 25 coatings of the present invention are sold to a consumer. In an embodiment, textiles of the present invention are used

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In the production of sportswear/apparel. In an embodiment, the silk coating of the present invention is applied to the lining of the garment. In an embodiment, the silk coating of the present invention is applied to the back of the textile. In an embodiment, the silk coating of the present invention is applied to the face, lining, or back of the garment. In an embodiment, the apparel is made partly of silk-coated textiles of the present invention and is made

<p dir="rtl">5 Partially made of uncoated textiles. In one embodiment, apparel made partly of silk-coated textiles and partly made of uncoated textiles combines an uncoated inert synthetic material and a silk-coated inert synthetic material. Examples of synthetic inert materials include, but are not limited to, polyester, polyamide, polyaramid, polytetrafluoroethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane, and polyethylene.</p>

<p dir="rtl">10 Glycol, ultra-high molecular weight polyethylene, high-pressure polyethylene, and mixtures thereof. In an embodiment, the apparel made partly of silk-coated textiles and partly made of uncoated textiles combines an elastomer material at least partly coated with a silk coating of the present invention. In an embodiment, the percentage of silk or elastomer material may be varied to obtain the desired shrink or wrinkle-resistant properties.</p>

15

20

25

In an embodiment, the silk coating of the present invention is visible. In an embodiment, the silk coating of the present invention applied to apparel helps control skin temperature. In an embodiment, the silk coating of the present invention applied to an apparel helps control the transfer of a fluid away from the skin. In an embodiment, the silk coating of the present invention applied to apparel has a smooth texture against the skin thus reducing the friction of the fabric against the skin. In an embodiment, the silk coating of the present invention applied to textiles has properties that impart at least one of wrinkle resistance, shrinkage resistance, and machine washability to textiles. In an embodiment, the silk-coated textiles of the present invention are 100% machine washable and dry-cleanable. In an embodiment, the silk-coated textiles of the present invention are 100% waterproof. In an embodiment, the silk-coated textiles of the present invention are wrinkle-resistant. In an embodiment, the silk-coated textiles of the present invention are shrink-resistant. In one embodiment, the textiles are coated with silk

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The current invention has waterproof, gas permeable, elastic, and a number of other qualities that are highly desirable in sportswear. In an embodiment, silk-coated textiles of the present invention are made from silk fabric of the present invention also comprising spun fibers of the present invention.

.LYCRA

10

15

In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a gas-permeable fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a water-resistant fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a shrink-resistant fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a machine washable fabric. In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention is a wrinkle-resistant fabric. In an embodiment, textiles are at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present invention that provide moisture and vitamins to the skin.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present invention is used for a textile coating. In one embodiment, the concentration of silk in the solution varies between about 0.1 and 20.0%. In one embodiment, the concentration of silk in the solution varies between about 0.1

<p dir="rtl">20 And 15.0%. In one embodiment, the concentration of silk in the solution varies between about 0.5 and 10.0%. in</p>

Typically, the concentration of silk in the solution ranges between about 1.0 and 5.0%. In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present invention is applied directly to a fabric. Alternatively, fine card or other fabric coating additives can be used. In an embodiment, additives can be added to an aqueous solution of the aqueous solution based on protein fragments

<p dir="rtl">25 Pure silk fibroin of the present invention is pre-coated (e.g. with alcohols) to further enhance the properties of the material.</p>

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In an embodiment, the silk coating of the present invention may have a pattern to make the properties of the silk best suited to the fabric. In an embodiment, a coating is applied to the fabric under tension and/or relaxation to change the penetration into the fabric.

In an embodiment, the silk coating of the present invention may be applied to the yarn plane, then producing a fabric

<p dir="rtl">5 Once the yarn is coated. In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present invention can be spun into fibers to make blended silk fabric with other materials known to the apparel industry.</p>

In an embodiment, a method for silk-coating a fabric comprises immersing the fabric in any of an aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments of the present invention. in

<p dir="rtl">10 Typically, a method of silk-coating fabric involves spraying. In one embodiment, a method for silk-coating fabric comprising chemical vapor deposition. In an embodiment, a method for silk-coating a fabric includes electrochemical coating. In an embodiment, a method for silk-coating a fabric comprises coating with a knife to spread any of an aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments of the present invention onto the fabric. The coated fabric can then be air dried,</p>

<p dir="rtl">15 Dry it under air flow, heat, or cross-tie it to the surface of the fabric. In an embodiment, the drying process includes treatment by additives and/or atmospheric conditions.</p>

Examples

Example (1): Tangential flow filtration (TFF) to remove solvent from solutions of dissolved silk

A variety of % Turkish azt silk has been produced through the use of tangential flow filtration (TFF).

<p dir="rtl">20 In all cases a 1% silk solution was used as the inlet feed stream. A range of 750-18,000 ml of 1% silk solution was used as the starting volume. Once below a specified level of residual LiBr was reached, the solution was ultrafiltrated to increase the concentration by removing water. See the following examples.</p>

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7.30% Silk Solution: A 7.30% silk solution was produced starting with 30-minute extraction batches for 100 g of silk cocoons/batch. The extracted silk fibers were then melted using a 9.3 M LiBr solution at 100°C in an oven at 100°C for 1 hour. 100 g silk fibers/batch were dissolved to produce 20% silk in LiBr. The melted silk was then diluted in

<p dir="rtl">5 LiBr to 1% silk and filtered through a 5 µm filter to remove large residues. 15,500 mL of 1% silk filter solution was used as the starting volume/dialysis filtration of TFF. Once LiBr was removed, the solution was ultrafiltered to a volume of approximately 1300 mL. 1262 ml of 7.30 silk was then collected. Water was added to the feed stream to help remove the remaining solution and then 547 ml of 3.91% silk was collected.</p>

<p dir="rtl">10 6.44% Silk Solution: A 6.44% silk solution was produced starting with 60-minute extraction batches from a mixture of 25, 33, 50, 75, and 100 g cocoons/batch. The extracted silk fibers were then melted using a 9.3 M solution of LiBr at 100°C in an oven at 100°C for 1 hour. 35, 42, 50, and 71 g/batch of silk fibers to produce 20% silk were dissolved in LiBr and collected. The silk dissolved in LiBr was then diluted to 1% silk and filtered through a 5-filter</p>

<p dir="rtl">15 Micrometer to remove large residues. 17,000 mL of 1% silk filter solution was used as the starting volume/dialysis filtration of TFF. Once LiBr was removed, the solution was ultrafiltered to a volume of approximately 3000 mL. 1490 ml of 6.44 silk was then collected. Water was added to the feed stream to help remove the remaining solution and then 1454 ml of 4.88% silk was collected.</p>

2.70% Silk Solution: A 2.70% Silk Solution was produced starting with 60-hour extraction batches.

<p dir="rtl">20 Minutes of 25g silk cocoons/batch. The melted silk fibers were then melted with a 9.3 M solution of LiBr at 100°C in an oven at 100°C for 1 h. 35.48 g of silk fibers/batch were dissolved to produce 20% silk in LiBr. The silk dissolved in LiBr was then diluted to 1% silk and filtered through a 5 μm filter to remove large residues. 1000 mL of 1% silk filter solution was used as the starting volume/dialysis filtration of TFF. Once LiBr was removed, filtration was done</p>

<p dir="rtl">25 Ultrasound the solution to a volume of about 300 ml. Then 312 ml of 2.7% silk was collected.</p>

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Example (2): Preparation of various types of silk gel

<tr><td colspan="8"><p dir="rtl">Table 17: Gel samples - Silk gel formula including additives, silk concentration and addition, gel formation conditions and times.</p></td></tr><tr><td><p dir="rtl">The number of days until the gel is formed</p></td><td><p dir="rtl">Temperature/processing</p></td><td><p dir="rtl">amount</p><p dir="rtl">add</p></td><td><p dir="rtl">addition</p></td><td><p dir="rtl">rate</p><p dir="rtl">Silk: Vitamin C</p></td><td><p dir="rtl">Mass</p><p dir="rtl">Vitamin C (g)</p></td><td><p dir="rtl">ml of 2% silk solution</p></td><td><p dir="rtl">name</p><p dir="rtl">the sample</p></td></tr><tr><td><p>8</p></td><td><p>RT</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>1</p></td></tr><tr><td><p>8</p></td><td><p>RT</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>1 :2.5</p></td><td><p>0.08</p></td><td><p>10</p></td><td><p>2</p></td></tr><tr><td><p>8</p></td><td><p>RT</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :1</p></td><td><p>0.2</p></td><td><p>10</p></td><td><p>3</p></td></tr><tr><td><p>14</p></td><td><p>RT</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>02 :1</p></td><td><p>0.4</p></td><td><p>10</p></td><td><p>4</p></td></tr><tr><td><p dir="rtl">no</p><p dir="rtl">something</p></td><td><p>RT</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>04 :1</p></td><td><p>0.8</p></td><td><p>10</p></td><td><p>5</p></td></tr><tr><td><p dir="rtl">About 39</p></td><td><p dir="rtl">refrigerator</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>6</p></td></tr><tr><td><p dir="rtl">About 39</p></td><td><p dir="rtl">refrigerator</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>1 :2.5</p></td><td><p>0.08</p></td><td><p>10</p></td><td><p>7</p></td></tr><tr><td><p dir="rtl">About 39</p></td><td><p dir="rtl">refrigerator</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :1</p></td><td><p>0.2</p></td><td><p>10</p></td><td><p>8</p></td></tr>

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<tr><td><p dir="rtl">no</p><p dir="rtl">something</p></td><td><p dir="rtl">refrigerator</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>02 :1</p></td><td><p>0.4</p></td><td><p>10</p></td><td><p>9</p></td></tr><tr><td><p dir="rtl">no</p><p dir="rtl">something</p></td><td><p dir="rtl">refrigerator</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>04 :1</p></td><td><p>0.8</p></td><td><p>10</p></td><td><p>10</p></td></tr><tr><td><p>8</p></td><td><p dir="rtl">RT/severe shaking at</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :1</p></td><td><p>0.2</p></td><td><p>10</p></td><td><p>11</p></td></tr><tr><td><p>3</p></td><td><p dir="rtl">Oven at 37°C</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>O-1</p></td></tr><tr><td><p>2</p></td><td><p dir="rtl">Oven at 50°C</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>O-2</p></td></tr><tr><td><p>4</p></td><td><p dir="rtl">Oven at 37°C</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :1</p></td><td><p>0.2</p></td><td><p>10</p></td><td><p>O-3</p></td></tr><tr><td><p>3</p></td><td><p dir="rtl">Oven at 50°C</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :1</p></td><td><p>0.2</p></td><td><p>10</p></td><td><p>O-4</p></td></tr><tr><td><p>5</p></td><td><p>RT</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :5</p></td><td><p>0.16</p></td><td><p>40</p></td><td><p>M</p></td></tr><tr><td><p>5</p></td><td><p>RT</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :5</p></td><td><p>0.16</p></td><td><p>40</p></td><td><p>D</p></td></tr><tr><td><p>7</p></td><td><p>RT</p></td><td><p dir="rtl">1 a point</p></td><td><p dir="rtl">Vitamin E</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>E1</p></td></tr><tr><td><p>7</p></td><td><p>RT</p></td><td><p dir="rtl">3 points</p></td><td><p dir="rtl">Vitamin E</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>E2</p></td></tr><tr><td><p dir="rtl">no</p><p dir="rtl">something</p></td><td><p>RT</p></td><td><p dir="rtl">1 a point</p></td><td><p dir="rtl">Vitamin E</p></td><td><p dir="rtl">nothing</p></td><td><p>0</p></td><td><p>10</p></td><td><p>E3</p></td></tr>

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<tr><td><p dir="rtl">no</p><p dir="rtl">something</p></td><td><p>RT</p></td><td><p dir="rtl">3 points</p></td><td><p dir="rtl">Vitamin E</p></td><td><p dir="rtl">nothing</p></td><td><p>0</p></td><td><p>10</p></td><td><p>E4</p></td></tr><tr><td><p>6</p></td><td><p>RT</p></td><td><p dir="rtl">300</p><p dir="rtl">µl</p></td><td><p dir="rtl">Lemon</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>L1</p></td></tr><tr><td><p>6</p></td><td><p>RT</p></td><td><p dir="rtl">300</p><p dir="rtl">µl</p></td><td><p dir="rtl">juice</p><p dir="rtl">Lemon</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>L2</p></td></tr><tr><td><p>5</p></td><td><p>RT</p></td><td><p dir="rtl">1000</p><p dir="rtl">µl</p></td><td><p dir="rtl">juice</p><p dir="rtl">Lemon</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>L3</p></td></tr><tr><td><p>6</p></td><td><p>RT</p></td><td><p dir="rtl">300</p><p dir="rtl">µl</p></td><td><p dir="rtl">Lemon</p></td><td><p dir="rtl">nothing</p></td><td><p>0</p></td><td><p>10</p></td><td><p>L4</p></td></tr><tr><td><p>7</p></td><td><p>RT</p></td><td><p dir="rtl">300</p><p dir="rtl">µl</p></td><td><p dir="rtl">juice</p><p dir="rtl">Lemon</p></td><td><p dir="rtl">nothing</p></td><td><p>0</p></td><td><p>10</p></td><td><p>L5</p></td></tr><tr><td><p>- 5</p><p>7</p></td><td><p>RT</p></td><td><p dir="rtl">2000</p><p dir="rtl">µl</p></td><td><p dir="rtl">juice</p><p dir="rtl">Lemon</p></td><td><p>01 :5</p></td><td><p>0.08</p></td><td><p>20</p></td><td><p dir="rtl">Jar</p><p dir="rtl">1</p></td></tr><tr><td><p>- 2</p><p>3</p></td><td><p>RT</p></td><td><p dir="rtl">1 a point</p></td><td><p dir="rtl">oil</p><p dir="rtl">Hashish</p><p dir="rtl">cumin</p></td><td><p>01 :5</p></td><td><p>0.02</p></td><td><p>5</p></td><td><p dir="rtl">Jar</p><p dir="rtl">2</p></td></tr><tr><td><p>7</p></td><td><p>RT</p></td><td><p dir="rtl">1 a point</p></td><td><p dir="rtl">oil</p><p dir="rtl">Gum gravel</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>R-1</p></td></tr><tr><td><p>7</p></td><td><p>/RT</p><p dir="rtl">the tube</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>T-1</p></td></tr>

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<tr><td><p>6</p></td><td><p>RT</p></td><td><p dir="rtl">1 a point</p></td><td><p dir="rtl">Rose oil</p></td><td><p>01 :5</p></td><td><p>0.04</p></td><td><p>10</p></td><td><p>RO-</p><p>1</p></td></tr><tr><td><p dir="rtl">no</p><p dir="rtl">something</p></td><td><p>RT</p></td><td><p dir="rtl">1 a point</p></td><td><p dir="rtl">Rose oil</p></td><td><p dir="rtl">nothing</p></td><td><p dir="rtl">nothing</p></td><td><p>10</p></td><td><p>RO-</p><p>2</p></td></tr>

Silk to vitamin C ratio

1 - 10 samples were used to examine the effect of the ratio of silk to vitamin C on the conversion of serum to gel. Samples 1-3 had less vitamin C quickly converted to gel than samples 4 and 5. All other conditions were kept constant. Samples 6-8 had less than 100% of vitamin C quickly converted to gel

<p dir="rtl">5 Samples 9 and 10. All other conditions were kept constant. It was concluded that reducing the ratio of silk to vitamin C (increasing the amount of vitamin C2), will prolong the gel formation time. For all ratios with small amounts of vitamin C, they did not change significantly over the days of switching to gel.</p>

Physical activation

Samples 3-11 were used to examine the effect of physical activation on the transformation of serum into gel. It was completed

10 Prepare each sample under the same conditions. Sample 11 was shaken vigorously for about 3 minutes after adding vitamin C. Otherwise, the treatment of samples 3 and 11 was similar. Shaking did not produce bubbles but significantly changed the gel production time.

Heat treatment

Samples 1, 3, 6, 8, 3-O, 2-O, 1-O, and 4-O were used to examine the effect of heat treatment.

15 Over the time the serum turns into gel. Samples 1, 6, 2-O and 1-O were identical except for heat treatment. Samples 3, 8, 4-O and 3-O were identical except for heat treatment. The two groups differed in the ratio of silk to vitamin C. The time for the serum to turn into a gel was directly related to the heat treatment, as the higher temperature resulted in a faster turn of the serum into a gel.

Solution volume

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Samples M, I, and D were used to examine the effect of solution volume on the time of serum turning into a gel. Samples M and D differed from sample I only in terms of increased solution volume. Samples M and D turned to gel in 5 days while sample I turned to gel in 8 days. It was noted that samples M and D in particular turned into gel on the same day as the gel formed, while sample I turned into gel at the end of the week.

<p dir="rtl">5 Extras</p>

Samples RO-1, R1, Jar 2, L5, L4, L3, L2, L1, E4, E3, E2, E1 and 2-RO were used to examine the effect of additives on the time of serum turning into a gel. Samples E4 - E1 contained vitamin E. . Samples E1 and E2 contained only vitamin C and only those samples turned into gel. Vitamin E can be added to a solution to form a gel but it appears that another addition is needed

<p dir="rtl">10 To produce gel. Samples L5 - L1 contained some form of lemon juice. Samples L1 and L4 contained juice directly from a lemon, while samples L3, L2, and L5 contained lemon juice from a plastic lemon container. Samples L4 and L5 did not contain vitamin C, while it was present in all other samples. All samples that produced gel. The amount and type of lemon juice had a small effect on the gel production time. Sample jar 2 contained lemongrass oil, which formed an albumin-like substance when...</p>

<p dir="rtl">15 Add it at the beginning. This sample also had vitamin C, but the gel production time was much faster than the other vitamin C samples. Sample R1 contained frankincense stone oil, which appeared to have dissolved, as well as vitamin C. The sample produced gel in a similar time frame to other samples with only vitamin C. Samples 1-RO and 2-RO contained rose oil while sample 1-RO contained vitamin C. The 1-RO sample that produced a gel showed that rose oil would not produce a gel any faster</p>

<p dir="rtl">20 on his own. In both cases, the rose oil was mixed and visible as clear bubbles.</p>

The solution of fragments based on hydrosilk fibroin and essential oils were miscible liquids. In one embodiment, to increase the aroma of a solution of silk fibroin-based fragments, without trapping oils within the solution, the solution is mixed with the base oil using a stirring column. The stirring column is rotated quickly so that some disturbance is observed in the mixture, thus creating contact between the oil

<p dir="rtl">25 aromatic base and molecules in the solution, adding scent to the solution before pouring the product from</p>

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solution, stop mixing and allow the oil to separate to the top of the solution. Distribution from the back of the solution to the final product allows it to be perfumed without any visible essential oil inside the final product.

Alternatively, a silk fibroin-based solution and essential oil can be mixed with or without additional ingredients, and/or an emulsifier to produce a formulation containing both ingredients.

<p dir="rtl">5 In an embodiment, mixing the solution as described above can reduce the gel production time if the solution is used to produce a gel formulation.</p>

The bowl

The two samples T1 and the jar were used to examine the effect of the pouring vessel on the time of turning the serum into a gel. The sample was poured into a glass jar while the T1 sample was poured into an aluminum tube. Produced both

<p dir="rtl">10 Both samples were gel, and the time for the serum to turn into gel was not affected.</p>

Summary

All silk solution treatments to produce a gel solution were performed in a conical tube at room temperature unless otherwise noted. The ratio of silk to vitamin C did not affect the ability of the solution to produce gel, as ratios above 1:2 did not produce gel, and a ratio of 1:2 took twice as long as

<p dir="rtl">15 Other smaller ratios (5:1, 2.5:1, 1:1). Temperature affected the gel production time as higher temperatures resulted in faster gel production times. Treatment at 50°C produced faster gel in two days. Treatment at 37°C produced It gelled faster in 3 days, and processing at room temperature produced gel in 5-8 days, and the sample stored in a refrigerator took at least 39 days to produce gel. The effects of the additives on gel production depended on the addition of vitamin E, frankincense oil, and frankincense. And oil</p>

<p dir="rtl">20 The roses are all based on the time of production of the gel. None of these additives prevented gel production or affected its production time. Each of them also requires the presence of vitamin C to produce gel. Lemon juice from fresh lemons, juice from previously squeezed lemons from a plastic lemon container, and lemongrass oil did not affect gel production. Without wanting to be bound by theory, it is believed that the lower pH due to these additions is the reason for their effect in reducing gel production time. Both types of lemonade were capable</p>

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It produces gel without the presence of vitamin C. This occurs in the same number of days as with vitamin C. Lemongrass oil was able to reduce the number of days of gel production to 2-3 days. All additives showed dissolution except lemongrass oil and rose oil. The rose oil remained in the form of clear bubbles, while the lemongrass oil partially dissolved and formed albumen-like masses. In some form, the

<p dir="rtl">5 Oils that have not completely dissolved are still suspended inside the gel as an additive. Physical activation by shaking the vessel of the solution from which it was poured and the volume of the solution did not affect the gel production time.</p><table border="1"><tbody><tr><td colspan="4"><p dir="rtl">Table 18: Concentration of vitamin C in different gel formulas</p></td></tr><tr><td colspan="2"><p dir="rtl">Vitamin C concentration (mg/mg)</p></td><td><p dir="rtl">Sample weight</p></td><td rowspan="2"><p dir="rtl">Sample information</p></td></tr><tr><td><p dir="rtl">Average</p></td><td><p dir="rtl">In the sample</p></td><td><p dir="rtl">(mg)</p></td></tr><tr><td rowspan="2"><p>3.2657</p></td><td><p>3.2511</p></td><td rowspan="2"><p>685.7</p></td><td rowspan="4"><p dir="rtl">Gum gravel (store at room temperature)</p></td></tr><tr><td><p>3.2804</p></td></tr><tr><td rowspan="2"><p>3.3334</p></td><td><p>3.3336</p></td><td rowspan="2"><p>638</p></td></tr><tr><td><p>3.3332</p></td></tr><tr><td rowspan="2"><p>2.877</p></td><td><p>2.8672</p></td><td rowspan="2"><p>464</p></td><td rowspan="4"><p dir="rtl">Lemongrass (store at</p><p dir="rtl">room temperature)</p></td></tr><tr><td><p>2.8868</p></td></tr><tr><td rowspan="2"><p>2.9051</p></td><td><p>2.9051</p></td><td rowspan="2"><p>465.5</p></td></tr><tr><td><p>2.9052</p></td></tr></tbody></table>

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<tr><td rowspan="2"><p>3.9147</p></td><td><p>3.9063</p></td><td rowspan="2"><p>654.2</p></td><td rowspan="4"><p dir="rtl">Gum gravel (store at room temperature, covered with foil)</p></td></tr><tr><td><p>3.923</p></td></tr><tr><td rowspan="2"><p>3.9374</p></td><td><p>3.9443</p></td><td rowspan="2"><p>649</p></td></tr><tr><td><p>3.9305</p></td></tr><tr><td rowspan="2"><p>3.8274</p></td><td><p>3.8253</p></td><td rowspan="2"><p>630.1</p></td><td rowspan="4"><p dir="rtl">Lemongrass (store at</p><p dir="rtl">room temperature covered with foil)</p></td></tr><tr><td><p>3.8295</p></td></tr><tr><td rowspan="2"><p>3.8253</p></td><td><p>3.8283</p></td><td rowspan="2"><p>660.4</p></td></tr><tr><td><p>3.8222</p></td></tr><tr><td rowspan="2"><p>5.1484</p></td><td><p>5.1516</p></td><td rowspan="2"><p>672.4</p></td><td rowspan="4"><p dir="rtl">Gum gravel (stored in refrigerator and covered with foil)</p></td></tr><tr><td><p>5.1352</p></td></tr><tr><td rowspan="2"><p>5.201</p></td><td><p>5.1984</p></td><td rowspan="2"><p>616.5</p></td></tr><tr><td><p>5.2036</p></td></tr>

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<tr><td rowspan="2"><p>5.1824</p></td><td><p>5.1871</p></td><td rowspan="2"><p>640.5</p></td><td rowspan="4"><p dir="rtl">Lemongrass (stored in refrigerator and covered with foil)</p></td></tr><tr><td><p>5.1776</p></td></tr><tr><td rowspan="2"><p>5.2126</p></td><td><p>5.2098</p></td><td rowspan="2"><p>627.7</p></td></tr><tr><td><p>5.2154</p></td></tr>

Example (3): Preparation of various types of silk gel

Additional gels can be prepared according to Tables (19, 20, 21, 22).

<tr><td colspan="2"><p dir="rtl">Table 19: Lemongrass gel</p></td></tr><tr><td><p>%2</p></td><td><p dir="rtl">% silk solution</p></td></tr><tr><td><p dir="rtl">Solution 100 mg/15 ml</p></td><td><p dir="rtl">The amount of vitamin C</p></td></tr><tr><td><p dir="rtl">20 microliter solution 15 ml</p></td><td><p dir="rtl">Quantity of lemongrass oil</p></td></tr>

<tr><td colspan="2"><p dir="rtl">Table 20: Gum stone gel</p></td></tr><tr><td><p>%2</p></td><td><p dir="rtl">% silk solution</p></td></tr><tr><td><p dir="rtl">Solution 100 mg/15 ml</p></td><td><p dir="rtl">The amount of vitamin C</p></td></tr><tr><td><p dir="rtl">Solution 20 µl/50 ml</p></td><td><p dir="rtl">Frankincense gravel oil quantity</p></td></tr>

Table 21: Lemongrass gel (50 ml)

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<tr><td><p>%2</p></td><td><p dir="rtl">% of silk solution (boiled for 60 minutes, 25 kDa)</p></td></tr><tr><td><p dir="rtl">Solution 12.82 mg/ml (total).</p><p dir="rtl">641 mg)</p></td><td><p dir="rtl">Quantity of Vitamin C (ascorbyl glucoside)</p></td></tr><tr><td><p dir="rtl">Solution 1.33 μmol ml</p></td><td><p dir="rtl">Lemongrass oil</p></td></tr><tr><td><p>4</p></td><td><p dir="rtl">pH</p></td></tr>

<tr><td colspan="2"><p dir="rtl">Table 21: Lemongrass gel (50 ml)</p></td></tr><tr><td><p>%2</p></td><td><p dir="rtl">% of silk solution (boiled for 60 minutes, 25 kDa)</p></td></tr><tr><td><p dir="rtl">Solution 12.82 mg/ml (total).</p><p dir="rtl">641 mg)</p></td><td><p dir="rtl">Quantity of Vitamin C (ascorbyl glucoside)</p></td></tr><tr><td><p dir="rtl">0.8 μmol ml solution</p></td><td><p dir="rtl">Frankincense gravel oil quantity</p></td></tr><tr><td><p>4</p></td><td><p dir="rtl">pH</p></td></tr>

The gels of the present invention can be made with silk solutions between 0.5 and 8%. Types can be made

Most of the current products are made with ascorbyl glucoside containing between about 0.67 and 15% w/w. The gels of the present invention are clear/white in colour. The gel types of the present invention can have a consistency

5 It can be spread or absorbed by the skin. The gels of the present invention may not produce a residue or oily sensation after being prescribed. The gels of the present invention do not turn brown over time.

Gels containing essential oils were prepared by diluting the silk solution of the present invention to 2%. Vitamin C was added to the solution and dissolved. Essential oil was added, stirred, and dissolved. The solution was divided into equal parts and placed in jars.

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Example (4): Coating fabric with water-based silk solutions

<tr><td colspan="6"><p dir="rtl">Table 23: Properties of silk solution</p></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">57 kilodalton</p></td><td><p dir="rtl">Molecular weight</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p>1.6</p></td><td><p dir="rtl">Multiple dispersion</p></td><td></td></tr><tr><td><p>%0.5</p></td><td><p>%1.0</p></td><td><p>%3.0</p></td><td><p>%5.0</p></td><td><p dir="rtl">% for silk</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td><p dir="rtl">Determinants</p><p dir="rtl">the operation</p></td></tr><tr><td></td><td></td><td></td><td></td><td><p dir="rtl">Extraction</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">30 minute</p></td><td><p dir="rtl">Boiling time</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">100°C</p></td><td><p dir="rtl">Boiling temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">60°C</p></td><td><p dir="rtl">Rinse temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p dir="rtl">Dissolving</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">100°C</p></td><td><p dir="rtl">LiBr temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">100°C</p></td><td><p dir="rtl">Oven temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">60 minute</p></td><td><p dir="rtl">Oven time</p></td><td></td></tr><tr><td colspan="6"><p dir="rtl">Table 24: Properties of silk solution</p></td></tr>

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<tr><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">25 kilodalton</p></td><td><p dir="rtl">Molecular weight</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p>2.4</p></td><td><p dir="rtl">Multiple dispersion</p></td><td></td></tr><tr><td><p>%0.5</p></td><td><p>%1.0</p></td><td><p>%3.0</p></td><td><p>%5.0</p></td><td><p dir="rtl">% for silk</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td><p dir="rtl">Determinants</p><p dir="rtl">the operation</p></td></tr><tr><td></td><td></td><td></td><td></td><td><p dir="rtl">Extraction</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">60 minute</p></td><td><p dir="rtl">Boiling time</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">100°C</p></td><td><p dir="rtl">Boiling temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">60°C</p></td><td><p dir="rtl">Rinse temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p dir="rtl">Dissolving</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">100°C</p></td><td><p dir="rtl">LiBr temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">100°C</p></td><td><p dir="rtl">Oven temperature</p></td><td></td></tr><tr><td></td><td></td><td></td><td><p dir="rtl">60 minute</p></td><td><p dir="rtl">Oven time</p></td><td></td></tr>

Apply silk solution and silk gel to spun fabric samples

Three 50 mm diameter fabric samples from each of three different fabric materials (cotton, polyester and nylon/LYCRA®) were placed in plastic containers, and approximately 0.3 ml of approximately 5.8% silk fibroin solution was deposited using a 1 ml syringe and an 18 gauge fabric needle on two samples. Of every material,

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It was left to settle for about 1 minute. Approximately 0.3 ml of an alcohol solution (containing methanol and ethanol) was then deposited using a 1 ml syringe and 30 gauge needle onto one of the silk-coated samples for each material.

In an additional experiment, silk gel was combined with frankincense essential oil (water, silk, glucoside).

5 Ascorbyl, frankincense gravel essential oil) on a stirrer and placed on half lengths of two 400 µm Lyocell pieces. One sample was then moistened with approximately 0.3 ml of alcohol.

Dip test in silk solution

Polyester fabric samples were dipped in silk fibroin solutions of different concentrations. The samples were placed in an incubator with air flow on a thin sheet and left to dry at about 22.5°C for about

10 15.5 hours. The weight change was measured before and after silk coating.

Table 25: Polyester fabric samples with silk coatings of the present invention

<tr><td><p dir="rtl">% to change</p><p dir="rtl">Average</p></td><td><p dir="rtl">% to change</p></td><td><p dir="rtl">Mass after coating (g)</p></td><td><p dir="rtl">Starting mass (g)</p></td><td><p dir="rtl">% of silk fibroin concentration</p></td></tr><tr><td rowspan="4"><p>%3-</p></td><td><p>4+</p></td><td><p>0.36</p></td><td><p>0.25</p></td><td rowspan="4"><p>1</p></td></tr><tr><td><p>10-</p></td><td><p>0.27</p></td><td><p>0.30</p></td></tr><tr><td><p>0</p></td><td><p>0.24</p></td><td><p>0.24</p></td></tr><tr><td><p>5-</p></td><td><p>0.21</p></td><td><p>0.22</p></td></tr><tr><td rowspan="3"><p>%15</p></td><td><p>20+</p></td><td><p>0.36</p></td><td><p>0.30</p></td><td rowspan="3"><p>3</p></td></tr><tr><td><p>11+</p></td><td><p>0.31</p></td><td><p>0.28</p></td></tr><tr><td><p>14+</p></td><td><p>0.33</p></td><td><p>0.29</p></td></tr>

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<tr><td></td><td><p>15+</p></td><td><p>0.34</p></td><td><p>0.29</p></td><td></td></tr><tr><td rowspan="4"><p>%16</p></td><td><p>16+</p></td><td><p>0.29</p></td><td><p>0.25</p></td><td rowspan="4"><p>5</p></td></tr><tr><td><p>18+</p></td><td><p>0.33</p></td><td><p>0.28</p></td></tr><tr><td><p>13+</p></td><td><p>0.35</p></td><td><p>0.31</p></td></tr><tr><td><p>15+</p></td><td><p>0.31</p></td><td><p>0.27</p></td></tr>

Silk solution spray test

A spray test was conducted to verify the hand-holding effect of a silk fibroin solution sprayed on a polyester fabric. A approximately 0.5% silk fibroin solution was applied to a 4 square polyester cloth

<p dir="rtl">x 4 using a spray gun from a distance of about 10 inches. Three passes were made from left to right, then from right to left (a total of 6 passes). The samples were placed in an oven at 50°C.</p>

On a thin aluminum foil over a water bath for about 1.5 hours. The method was repeated from a second polyester fabric sample with a approximately 5.8% silk fibroin solution sprayed. No change in hand holding was observed for samples sprayed with either 0.5% or 5.8% solutions. An expected increase in material smoothness was observed for samples sprayed with either 0.5% and 5.8% solutions.

<p dir="rtl">10 Example (5): The process of painting a fabric as appropriate</p>

Table 26: Coating processes

<p dir="rtl">1- Spraying</p>

1-1- Materials for painting.

Hobby Lobby part 132894 36 x 24 1-1-1- Cork board

2-1-1- Cover the cork board with an interlocking polyester cloth

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3-1-1- Spread horse for load

4-1-1- Several clips to attach the cork board to the sawing horse

5-1-1- Double filter to remove oil residue from the compressor and dehumidification salt

Iwata eclipse MP-CS 1-1-6- Air brush

7-1-1- Husky pressure system equipped with a 30.3 liter tank.

8-1-1- Desk staples to secure fabric to cork board # Hobby Lobby part 523456

2-1- Materials for preparation

1-2-1- Scissors

2-2-1- Ruler

AWS model Pnx-203 1-2-3- balance

3-1- Materials for drying

1-3-1- Wolf stove frozen at 150°F to maintain a temperature of 71-78°C with a fan system

2-3-1- Flat baking sheet

3-3-1- Aluminum foil

4-3-1- Temperature gauge with SC 307/T probe

4-1- Implementation

1-4-1- Place the fabric you want to paint on the face of the cork board covered with the polyester fabric.

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2-4-1- Attach the top with staples to the cork board

3-4-1- Equip the compressor with oil and moisture filters

4-4-1- Set the air pressure at 55 psi2

5-4-1- Carry the solution in a gun equipped with an airbrush

6-4-1- Place the airbrush gun approximately 10 inches from the board

7-4-1- Pull the trigger of the airbrush gun and spray 2 inches from side to side of the fabric to be painted.

8-4-1- Remove the pin from the cork board and place the coated fabric on the aluminum foil

9-4-1- Place the coated fabric in the oven for 30 - 60 minutes at 150°C.

<p dir="rtl">2- Spraying/stenciling</p>

1-2- Materials for painting

Hobby Lobby part 132894 36 x 24 2-1-1- Cork board

2-1-2- Cover the cork board with an interlocking polyester cloth

3-1-2- Spreading horse for load

4-1-2- Several clips to attach the cork board to the sawing horse

5-1-2- Double filter to remove oil residue from the compressor and dehumidifying salt

Iwata eclipse MP-CS 2-1-6- Air brush

7-1-2- Husky pressure system equipped with a 30.3 liter tank.

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8-1-2- Desk staples to secure fabric to cork board # Hobby Lobby part 523456

9-1-2- Stencil Pattern 0.020 x 24 x 12 SKU#75244 Lincaine

Hobby Lobby

2-2- Materials for preparation

1-2-2- Scissors

2-2-2- Ruler

AWS model Pnx-203 2-2-3- Balancer

3-2- Materials for drying

1-3-2- Wolf stove frozen at 150°F to maintain a temperature of 71-78°C with a fan system

2-3-2- Flat baking sheet

3-3-2- Aluminum foil

4-3-2- Temperature gauge with SC 307/T probe

4-2- Implementation

1-4-2- Place the fabric you want to paint on the face of the cork board covered with the polyester fabric.

2-4-2- Place the stencil pattern on the face of the fabric.

3-4-2- Attach the stencil to the cork board with a pin

4-4-2- Equip the compressor with oil and moisture filters

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5-4-2- Set the air pressure at 55 psi

6-4-2- Carry the solution in a gun equipped with an airbrush

7-4-2- Place the airbrush gun approximately 10 inches from the board

8-4-2- Pull the trigger of the airbrush gun and spray 2 inches from side to side of the fabric to be painted.

9-4-2- Remove the pin from the cork board and place the coated fabric on the aluminum foil. 10-4-2- Place the coated fabric in the oven for 30 - 60 minutes at 150°C.

<p dir="rtl">3- Sieve printing</p>

1-3- Materials for painting

Hobby Lobby part 132894 36 x 24 3-1-1-Cork board

2-1-3- Cover the cork board with an interlocking polyester cloth

3-1-3- Spreading horse for load

4-1-3- Several clips to attach the cork board to the sawing horse

5-1-3- 12 x 18 sieve print frame part number 710 made by

speedball

6-1-3- Silicone spatula

2-3- Materials for preparation

1-2-3- Scissors

2-2-3- Ruler

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AWS model Pnx-203 3-2-3- Balancer

3-3- Materials for drying

1-3-3- Wolf stove frozen at 150°F to maintain a temperature of 71-78°C with a fan system

2-3-3- Flat baking sheet

3-3-3- Thin aluminum

4-3-3- Temperature gauge with SC 307/T probe

4-3- Implementation

1-4-3- Place the fabric you want to paint on the face of the cork board covered with the polyester fabric.

2-4-3- Place the print frame with a sieve on the surface of the fabric

3-4-3- Load the solution onto one edge of the printing frame with a sieve

4-4-3- Using a silicone spatula, move the solution across the printing frame with a sieve

5-4-3- Remove the print frame with a sieve and place the coated fabric on an aluminum foil

6-4-3- Place the coated fabric in the oven for 30 - 60 minutes at 150°C.

<p dir="rtl">4- Bathroom</p>

1-4- Materials for painting

Hobby Lobby part 132894 36 x 24 4-1-1- Cork board

2-1-4- Cover the cork board with an interlocking polyester cloth

3-1-4- Horsepower sawing for load

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4-1-4- Several clips to attach the cork board to the sawing horse

Item #: 170418 Model #: LOWES0- 4-1-5- Paint Tray

PK170418 at Lowes Hardware

6-1-4- Lattice making machine 4590-15 # Imperia model

2-4- Materials for preparation

1-2-4- Scissors

2-2-4- Ruler

AWS model Pnx-203 4-2-3- Balancer

3-4- Materials for drying

1-3-4- Wolf stove frozen at 150°F to maintain a temperature of 71-78°C with a fan system

2-3-4- Flat baking sheet

3-3-4- Thin aluminum

4-3-4- Temperature gauge with SC 307/T probe

4-4- Implementation

1-4-4- Hold the silk solution inside the eye of the paint tray

2-4-4- Immerse the sample of fabric to be painted in the silk solution until it is completely saturated

3-4-4- Pass the saturated fabric between two pressure rollers (capillary making machine) to remove any excess solution.

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4-4-4- Place the coated fabric on an aluminum foil

5-4-4- Place the coated fabric in an oven for 30 - 60 minutes at 150°C.

Products produced using the previously mentioned coating processes are tested to determine the cumulative unidirectional transfer capacity (or coefficient) and other properties using Test Method 195 - 2012 of the Association for Textiles, Apparel and Materials Professionals (AATCC) for Treating, Evaluating, and Classifying the Control of Liquid Humidity Properties of Textile Fabrics. Details of test methods are available from the AATCC and a summary

<p dir="rtl">5 The methods and calculations are there. The ART (face surface) and ARB (back surface) absorption rate was defined as the average velocity of liquid moisture absorption for the face and back surfaces of the sample during the initial change of water content during the test. The cumulative unidirectional transfer capacity (R) was defined as the difference between the area Liquid moisture water content curves for the front and back surfaces (sample with respect to time). 10 The back surface (B) is defined for the purposes of the test as the side of the sample placed down against the electrical sensor. The back is the side of the fabric that is</p>

The outer exposed surface of a garment when worn or a product when used. The face surface (T) of the test ground was defined as the side of the specimen placed on the back electrode sensor, i.e. facing the face sensor. It is the side of the fabric that will come into contact with the skin when wearing the garment or using the product. The maximum wetted radius (MWRT) and (MWRB) (in mm) 15 was defined as the largest ring radius measured at the face and back surfaces. Humidity control, for a liquid humidity control test, is defined as the engineered or inherent transfer of aqueous fluids such as sweat or water (associated with comfort). It includes both vapor and liquid forms of water. The overall liquid humidity control capability (OMMC) is calculated as, The overall capacity factor of a fabric to transfer liquid moisture is calculated by combining three measured performance characteristics: the rate of absorption of liquid moisture over the 20 back surface (ARB), the unidirectional liquid transfer capacity (R), and the maximum liquid moisture diffusion speed.

On the roof back (SSB). The speed of diffusion (SSi) is defined as the cumulative rate of wetting of a surface from the center of the sample as the tested solution is dripped to the maximum wetted radius. The total water content (%U) is defined as the sum of the percentage water content of the two surfaces

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Face and back. The wetting time (WTT) for the face surface and (WTB) for the back surface is defined as the time in seconds when the face and back surfaces of the sample begin to get wet after the start of the test.

A moisture control tester (MMT) is used to perform the test. The cumulative unidirectional fluid transport capacity (R) is calculated as: [area (UB) - area (UT)]/test time

<p dir="rtl">5 Total. OMMC is calculated as: *OMMC=C1*ARB_ndv+C2*Rnvd+C3 SSB_ndv, where C1, C2, C3 are weighted values of Rndv, ARB-ndv and SSB_ndv; (ARB) = rate of absorption, (R) cumulative unidirectional transmission capacity, and (SSB) = speed of propagation. Detailed calculations for these and other parameters are provided in Method 195 - 2012 of the AATCC Test Method.</p>

10 A description of the samples used is given in Table 27.

Table 27: Description of samples

<tr><td><p dir="rtl">the description</p></td><td><p dir="rtl">Sample identification number</p></td></tr><tr><td><p dir="rtl">Without coating (polyester)</p></td><td><p>15051201</p></td></tr><tr><td><p dir="rtl">Spray coating of 1% silk solution on sample 15051201</p></td><td><p>15051301</p></td></tr><tr><td><p dir="rtl">Spray coating of 0.1% silk solution on sample 15051201</p></td><td><p>15051302</p></td></tr><tr><td><p dir="rtl">15051201 Spray coating 0.05% silk solution on</p><p dir="rtl">a sample</p></td><td><p>15051303</p></td></tr><tr><td><p dir="rtl">Stencil coating by spraying a 1% silk solution onto sample 15051201</p></td><td><p>15051304</p></td></tr>

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<tr><td><p dir="rtl">Stencil coating by spraying 0.1% silk solution on sample 15051201</p></td><td><p>15051305</p></td></tr><tr><td><p dir="rtl">Stencil coating by spraying 0.05% silk solution on sample 15051201</p></td><td><p>15051306</p></td></tr><tr><td><p dir="rtl">Bath coating of 1% silk solution on sample 1505201</p></td><td><p>15051401</p></td></tr><tr><td><p dir="rtl">Bath coating of 0.1% silk solution on sample 1505201</p></td><td><p>15051402</p></td></tr><tr><td><p dir="rtl">Bath coating of 0.05% silk solution on sample 1505201</p></td><td><p>15051403</p></td></tr><tr><td><p dir="rtl">Sieve printing for PureProc on sample 1505201</p></td><td><p>15051404</p></td></tr><tr><td><p dir="rtl">Finished without kinking</p></td><td><p>15042001</p></td></tr><tr><td><p dir="rtl">Semi-finished before final preparation</p></td><td><p>15042002</p></td></tr><tr><td><p dir="rtl">Finished with wicking</p></td><td><p>15042003</p></td></tr><tr><td><p dir="rtl">1% silk solution spray paint (15042001) finish</p><p dir="rtl">Without kinking</p></td><td><p>15042101</p></td></tr><tr><td><p dir="rtl">0.1% silk solution spray paint (15042001) finish without wicking</p></td><td><p>15042102</p></td></tr><tr><td><p dir="rtl">Stencil paint with 1% silk solution (15042001) finish without wicking</p></td><td><p>15061206</p></td></tr><tr><td><p dir="rtl">Bath paint with 1% silk solution (15042001) finished</p><p dir="rtl">Without kinking</p></td><td><p>15061207</p></td></tr>

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<tr><td><p dir="rtl">Stencil paint with 0.1% silk solution (15042001) finish without wicking</p></td><td><p>15061205</p></td></tr><tr><td><p dir="rtl">Bath paint with silk solution 0.1 (15042001) finished</p><p dir="rtl">Without kinking</p></td><td><p>15061209</p></td></tr><tr><td><p dir="rtl">Spray painting with 1% silk solution (15042002) semi-finished before final preparation</p></td><td><p>15061201</p></td></tr><tr><td><p dir="rtl">Stencil paint with 1% silk solution (15042002) semi-finished before final preparation</p></td><td><p>15061203</p></td></tr><tr><td><p dir="rtl">Paint with a 1% silk solution bath (15042002) Semi-finishing before final preparation</p></td><td><p>15061208</p></td></tr><tr><td><p dir="rtl">Spray painting with 0.1% silk solution (15042002) semi-finished before final preparation</p></td><td><p>15061202</p></td></tr><tr><td><p dir="rtl">Stencil paint with 1% silk solution (15042002) semi-finished before final preparation</p></td><td><p>15061204</p></td></tr><tr><td><p dir="rtl">Paint with a 0.1% silk solution bath (15042002) Semi-finished before final preparation</p></td><td><p>15061210</p></td></tr>

The test results are shown in Figures (57a-86b) and show the superstress of a silk-coated fabric, including the superstress relative to the cumulative unidirectional transfer capacity (coefficient) and the total humidity control capacity.

Example (6): Antimicrobial properties of silk coatings on fabrics

The antimicrobial properties of the silk coatings were tested on four materials: cotton jersey/LYCRA (15051201)/cotton jersey/LYCRA with silk fibroin solution (SFS) bath coating.

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(15070701), post-finish polyester/LYCRA (15042003), and 1% SFS bath coating semi-finish polyester/LYCRA (15070702) (where LYCRA is the trade name for the polyester-polyurethane copolymer). AATCC Test Method 2012-100 was used to evaluate antimicrobial finishes on woven materials. details

5 The test method is available from the AATCC. Briefly, the tests were performed using trypsinized soybean broth as the growth medium, a 4-stratified sample volume, autoclave sterilization, and 100 ml ligtin broth with Tween for neutralization. The target inoculum level was 1-2 510 x culture units/ml, 5% nutrient broth. As a carrier medium and dilution medium for inoculation, contact time 18-24 hours, temperature 37 ± °C,

10 The test results are summarized in Table 28 and illustrated in Figures (87-92), and they demonstrate the superior antimicrobial pressure of silk-coated fabrics.

Table (28): Antimicrobial test results

<tr><td colspan="3"><p dir="rtl">Results: cfu/sample</p></td><td colspan="2"></td></tr><tr><td><p dir="rtl">% for deficiency</p></td><td><p dir="rtl">sand</p><p dir="rtl">Touch 24</p><p dir="rtl">hour</p></td><td><p dir="rtl">rammer</p><p dir="rtl">contact</p><p dir="rtl">zero</p></td><td><p dir="rtl">Bacteria</p></td><td><p dir="rtl">Sample number</p></td></tr><tr><td><p dir="rtl">3883.74</p><p dir="rtl">٥/٠</p></td><td><p>4.90ΕΟ</p><p>6</p></td><td><p>1.23+0</p><p>5</p></td><td><p>Staphylococcus aureus ATCC 6538</p></td><td rowspan="2"><p>1505120</p><p>1</p></td></tr><tr><td><p dir="rtl">2869.70</p><p dir="rtl">٥/٠</p></td><td><p>4.90ΕΟ</p><p>6</p></td><td><p>1.65+0</p><p>5</p></td><td><p>Klebsiella pneumoniae ATCC 4352</p></td></tr>

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<tr><td><p>3883.74</p><p>%</p></td><td><p>4.90Ε+0</p><p>6</p></td><td><p>1.23Ε+0</p><p>5</p></td><td><p>aphylocaccus aureus ATCC 6538</p></td><td rowspan="2"><p>1507070)</p><p>1</p></td></tr><tr><td><p dir="rtl">2869.70</p><p dir="rtl">٥/٠</p></td><td><p>4.90Ε+0</p><p>6</p></td><td><p>1.65+0</p><p>5</p></td><td><p>ebsiella pneumoniae ATCC 4352</p></td></tr><tr><td><p dir="rtl">3883.74</p><p dir="rtl">٥/٠</p></td><td><p>4.90ΕΟ</p><p>6</p></td><td><p>1.23+0</p><p>5</p></td><td><p>phylococcus aureus ATCC 5538</p></td><td rowspan="2"><p>3</p></td></tr><tr><td><p dir="rtl">2869.70</p><p dir="rtl">٥/٠</p></td><td><p>4.90ΕΟ</p><p>6</p></td><td><p>1.65+0</p><p>5</p></td><td><p>ebsiella pneumoniae AT C 4352</p></td></tr><tr><td><p dir="rtl">٥/٠91.63</p></td><td><p>1.03ΕΟ</p><p>4</p></td><td><p>1.23+0</p><p>5</p></td><td><p>]phylococcus aureus ATCC 6538</p></td><td rowspan="2"><p>1507070</p><p>2</p></td></tr><tr><td><p>%40.91-</p></td><td><p>2.33ΕΟ</p><p>5</p></td><td><p>1.65+0</p><p>5</p></td><td><p>ebsiella pneumoniae ATCC 4352</p></td></tr>

Example (7): Methods for preparing fabrics with silk coatings

A method for preparing an aqueous solution from an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of approximately 6 to 16 kDa includes the steps of degumming the silk source by adding the silk source to a boiling (00 M) aqueous solution of sodium carbonate for a period of time.

5 Process between about 30 and 60 minutes; removing sericin from the solution to produce a silk fibroin extract containing undetectable levels of sericin; And filtering the silk from the silk fibroin extract, and dissolving the silk fibroin extract in a solution of lithium bromide with a starting temperature of

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Put the silk fibroin extract in a lithium bromide solution. It goes between about 60 and 140 AD; Keep the silk fibroin-lithium bromide solution in an oven at a temperature of about 140°C for at least 1 hour. Removal of lithium bromide from silk fibroin extract; Producing an aqueous solution from silk protein fragments. The aqueous solution includes: fragments with an average molecular weight of about 6

<p dir="rtl">5 and 16 kDa, wherein the aqueous solution of the aqueous solution of pure silk fibroin-based protein fragments comprises a polydispersity between about 1.5 and 3.0. The solution from the aqueous solution of pure silk fibroin-based protein fragments may include lithium bromide residues less than</p>

10

15

20

300 ppm as measured using a high-pressure liquid chromatography lithium bromide determination experiment. An aqueous solution of pure silk fibroin-based protein fragments may contain a lithium carbonate residue of less than 100 ppm as measured using a high-pressure liquid chromatography-based sodium chromatography experiment. The method may also include adding a therapeutic agent to the aqueous solution of the aqueous solution of pure silk fibroin-based protein fragments. The method may further comprise adding a selected molecule of an antioxidant or enzyme to an aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. The method may also include vitamin to aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. The vitamin can be vitamin C or a derivative of it. The method may also include adding an alpha hydroxy acid to an aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. The alpha hydroxy acid can be chosen from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. The method may also include the addition of hyaluronic acid or...

Form its salts at a concentration between about 0.5 and 10.0% to the aqueous solution of the aqueous solution of protein fragments based on pure silk fibroin. The method may also include adding at least one of zinc oxide or titanium dioxide.

Comprising a method for preparing an aqueous solution from an aqueous solution of protein fragments based on silk fibroin

25 Pure, it has an average molecular weight that ranges between about 17 and 38 kilodaltons according to the removal steps.

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Gum a silk source by adding the silk source to a boiling aqueous solution (100 mM) of sodium carbonate for a processing time of about 30 to 60 minutes; removing sericin from the solution to produce a silk fibroin extract containing undetectable levels of sericin; and filtering the silk from the fibroin extract

Silk, and dissolving the silk fibroin extract in a solution of lithium bromide having a starting temperature of

<p dir="rtl">5 Put the silk fibroin extract in a lithium bromide solution. It ranges between about 80 and 140 degrees Celsius; Keep the silk fibroin-lithium bromide solution in an oven at a temperature of about 140°C for at least 1 hour. Removal of lithium bromide from silk fibroin extract; And producing an aqueous solution from silk protein fragments. The aqueous solution includes: fragments with an average molecular weight between about 17 and 38 kilodalto, and where the aqueous solution includes the aqueous solution of protein fragments based on</p>

<p dir="rtl">10 Pure silk fibroin has a polydispersity between about 1.5 and 3.0. An aqueous solution of pure silk fibroin-based protein fragments may contain a lithium bromide residue of less than 300 ppm as measured using a high-pressure liquid chromatography-based lithium bromide determination experiment. An aqueous solution of pure silk fibroin-based protein fragments may contain a lithium carbonate residue of less than 100 ppm as measured</p>

15

20

Using an experiment to determine sodium Arf chromatography using high-pressure liquid Arf chromatography. The method may also include adding a therapeutic agent to the aqueous solution of the aqueous solution of pure silk fibroin-based protein fragments. The method may further comprise adding a selected molecule of an antioxidant or enzyme to an aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. The method may also include vitamin to aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. The vitamin can be vitamin C or a derivative of it. The method may also include adding an alpha hydroxy acid to an aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. The alpha hydroxy acid can be chosen from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. The method may also include the addition of hyaluronic acid or...

25 Image of its salts at a concentration between about 0.5 and 10.0% to the aqueous solution of the aqueous solution of

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Protein fragments based on pure silk fibroin. The method may also include adding at least one of zinc oxide or titanium dioxide.

A method for preparing an aqueous solution from an aqueous solution of pure silk fibroin-based protein fragments having an average molecular weight of about 39 to 80 kDa includes the steps of removing

5 Gum a silk source by adding the silk source to a boiling aqueous solution (100°C) of sodium carbonate for a processing time of about 30 minutes; removing sericin from the solution to produce a silk fibroin extract containing undetectable levels of sericin; filtering the silk from the silk fibroin extract, and dissolving the fibroin extract Silk in a solution of lithium bromide has an initial temperature when placing silk fibroin extract in a solution of lithium bromide, which ranges between about 80 and 140 degrees Celsius

<p dir="rtl">10 Silk fibroin-lithium bromide solution in an oven at a temperature between about 10 and 100°C for a period of at least 1 hour; Removal of lithium bromide from silk fibroin extract; And producing an aqueous solution from silk protein fragments, wherein the aqueous solution includes: fragments with an average molecular weight between about 40 and 65 kilodalto, and wherein the aqueous solution includes the aqueous solution of protein fragments based on pure silk fibroin with a polydispersity between about 1.5 and 3.0. The solution may include:</p>

<p dir="rtl">15 of aqueous solution of pure silk fibroin-based protein fragments on lithium bromide residues between about 10 and 300 ppm as measured using a high-pressure liquid chromatography lithium bromide determination experiment. An aqueous solution of pure silk fibroin-based protein fragments may contain a lithium carbonate residue of less than 100 ppm as measured using a high-pressure sodium carbonate determination experiment using high-pressure liquid chromatography.</p>

<p dir="rtl">20 The method may also include adding a therapeutic agent to the aqueous solution of the aqueous solution of pure silk fibroin-based protein fragments. The method may further comprise adding a selected molecule of an antioxidant or enzyme to an aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. The method may also include vitamin to aqueous solution of an aqueous solution of pure silk fibroin-based protein fragments. A vitamin can be...</p>

<p dir="rtl">25 Vitamin C or a derivative thereof. The method may also include adding an alpha hydroxy acid to...</p>

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Aqueous solution of aqueous solution of pure silk fibroin-based protein fragments. The alpha hydroxy acid can be chosen from a group consisting of glycolic acid, lactic acid, tartaric acid, and citric acid. The method may also include adding hyaluronic acid or its salt form at a concentration between about 0.5 and 10.0% to the aqueous solution of the aqueous solution of

<p dir="rtl">5 Protein fragments based on pure silk fibroin. The method may also include adding at least one of zinc oxide or titanium dioxide.</p>

Example (8): Differentiation of silk coatings on polyester

A summary of results from studies of silk-on-polyester coatings is given in Tables 29 and 30.

The results shown in Figures (93, 94) show that the unidirectional transmission coefficient was maintained

<p dir="rtl">10 Accumulation and OMMC performance even at 60 wash cycles. The results of the addition tests are shown in the figures</p>

<p dir="rtl">(95-102). The antimicrobial pressure of the silk-coated polyester fabric was maintained for between 25 and 50 wash cycles as shown in Figures (103, 104). The results demonstrate the amazing improvement in moisture control properties, in addition to the surprising result of survival Improved properties across multiple washing cycles.</p>

15 Table 29:

<tr><td><p dir="rtl">Total humidity control ability</p><p>OM</p><p>MC</p></td><td><p dir="rtl">Cumulative unidirectional transmission coefficient (%)</p></td><td><p dir="rtl">Spread speed in the back (mm/s)</p></td><td><p dir="rtl">Spread speed in the face (mm/s)</p></td><td><p dir="rtl">Half the maximum wetting diameter</p><p dir="rtl">He shouted for the moment</p><p dir="rtl">^R)millimeter(</p></td><td><p dir="rtl">Half the diameter of the seasoning (mm)</p></td><td><p dir="rtl">Back absorption rate (%/sec)</p></td><td><p dir="rtl">Facial absorption rate (%/sec)</p></td><td><p dir="rtl">The time when the back gets wet</p><p dir="rtl">t (second)</p></td><td><p dir="rtl">Face wet time (seconds)</p></td><td><p dir="rtl">Raw data</p></td></tr>

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<tr><td><p>0.27</p></td><td><p>133.</p><p>26</p></td><td><p>1.2</p><p>2</p></td><td><p>0.9</p><p>0</p></td><td><p>5</p></td><td><p>5</p></td><td><p>28.7</p><p>3</p></td><td><p>7.24</p></td><td><p>3.9</p><p>5</p></td><td><p>5.6</p><p>3</p></td><td><p dir="rtl">I died in the middle</p></td></tr><tr><td><p>0.06</p></td><td><p>34.8</p><p>1</p></td><td><p>0.1</p><p>2</p></td><td><p>0.2</p><p>0</p></td><td><p>0</p></td><td><p>0</p></td><td><p>8.62</p></td><td><p>1.46</p></td><td><p>0.3</p><p>8</p></td><td><p>1.2</p><p>0</p></td><td><p dir="rtl">Deviation of measurement</p></td></tr><tr><td><p>0.21</p></td><td><p>0.26</p></td><td><p>0.0</p><p>9</p></td><td><p>0.2</p><p>2</p></td><td><p>0</p></td><td><p>0</p></td><td><p>0.30</p></td><td><p>0.20</p></td><td><p>0.1</p><p>0</p></td><td><p>0.2</p><p>1</p></td><td><p>CV</p></td></tr><tr><td><p>0.22</p></td><td><p>144.</p><p>84</p></td><td><p>0.6</p><p>8</p></td><td><p>0.4</p><p>6</p></td><td><p>5</p></td><td><p>5</p></td><td><p>8.55</p></td><td><p>4.82</p></td><td><p>7.9</p><p>6</p></td><td><p>23.</p><p>87</p></td><td><p dir="rtl">I died in the middle</p></td></tr><tr><td><p>0.03</p></td><td><p>27.7</p><p>1</p></td><td><p>0.2</p><p>3</p></td><td><p>0.2</p><p>8</p></td><td><p>0</p></td><td><p>0</p></td><td><p>2.94</p></td><td><p>0.84</p></td><td><p>3.3</p><p>0</p></td><td><p>31.</p><p>51</p></td><td><p dir="rtl">Deviation of measurement</p></td></tr><tr><td><p>0.14</p></td><td><p>0.19</p></td><td><p>0.3</p><p>3</p></td><td><p>0.6</p><p>1</p></td><td><p>0</p></td><td><p>0</p></td><td><p>0.34</p></td><td><p>0.17</p></td><td><p>0.4</p><p>1</p></td><td><p>1.3</p><p>2</p></td><td><p>CV</p></td></tr><tr><td><p>0.22</p></td><td><p>124.</p><p>05</p></td><td><p>1.0</p><p>5</p></td><td><p>0.8</p><p>3</p></td><td><p>5</p></td><td><p>5</p></td><td><p>17.2</p><p>2</p></td><td><p>7.36</p></td><td><p>4.5</p><p>9</p></td><td><p>6.0</p><p>9</p></td><td><p dir="rtl">I died in the middle</p></td></tr><tr><td><p>0.02</p></td><td><p>11.7</p><p>0</p></td><td><p>0.0</p><p>9</p></td><td><p>0.1</p><p>7</p></td><td><p>0</p></td><td><p>0</p></td><td><p>3.28</p></td><td><p>2.98</p></td><td><p>0.4</p><p>4</p></td><td><p>1.6</p><p>1</p></td><td><p dir="rtl">Deviation of measurement</p></td></tr>

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<tr><td><p>0.09</p></td><td><p>0.09</p></td><td><p>0.0</p><p>9</p></td><td><p>0.2</p><p>0</p></td><td><p>0</p></td><td><p>0</p></td><td><p>0.19</p></td><td><p>0.40</p></td><td><p>0.1</p><p>0</p></td><td><p>0.2</p><p>6</p></td><td><p>CV</p></td></tr><tr><td><p>0.13</p></td><td><p>58.8</p><p>1</p></td><td><p>0.5</p><p>3</p></td><td><p>0.3</p><p>9</p></td><td><p>5</p></td><td><p>5</p></td><td><p>7.80</p></td><td><p>6.84</p></td><td><p>11.</p><p>64</p></td><td><p>25.</p><p>20</p></td><td><p dir="rtl">I died in the middle</p></td></tr><tr><td><p>0.03</p></td><td><p>26.5</p><p>6</p></td><td><p>0.2</p><p>7</p></td><td><p>0.3</p><p>0</p></td><td><p>0</p></td><td><p>0</p></td><td><p>5.70</p></td><td><p>3.38</p></td><td><p>6.3</p><p>6</p></td><td><p>28.</p><p>06</p></td><td><p dir="rtl">Deviation of measurement</p></td></tr><tr><td><p>0.25</p></td><td><p>0.45</p></td><td><p>0.5</p><p>1</p></td><td><p>0.7</p><p>7</p></td><td><p>0</p></td><td><p>0</p></td><td><p>0.73</p></td><td><p>0.49</p></td><td><p>0.5</p><p>5</p></td><td><p>1.1</p><p>1</p></td><td><p>CV</p></td></tr>

Table 30:

<tr><td><p dir="rtl">Total humidity control ability</p><p>OMM</p><p>C</p></td><td><p dir="rtl">Unidirectional transfer coefficient</p><p dir="rtl">H</p><p dir="rtl">Cumulative (%)</p></td><td><p dir="rtl">Spread speed in the back (mm/s)</p></td><td><p dir="rtl">Spread speed in the face (mm/s)</p></td><td><p dir="rtl">Maximum wetting radius</p><p dir="rtl">Height of the back (mm)</p></td><td><p dir="rtl">Half the diameter of the seasoning (mm)</p></td><td><p dir="rtl">Back absorption rate (%/sec)</p></td><td><p dir="rtl">Facial absorption rate (%/sec)</p></td><td><p dir="rtl">The time when the back gets wet</p><p dir="rtl">t (second)</p></td><td><p dir="rtl">Face wet time (seconds)</p></td><td><p dir="rtl">Raw data</p></td></tr><tr><td><p>0.29</p></td><td><p>62.3</p><p>7</p></td><td><p>1.36</p></td><td><p>1.37</p></td><td><p>5</p></td><td><p>5</p></td><td><p>56.9</p><p>0</p></td><td><p>37.3</p><p>0</p></td><td><p>3.4</p><p>8</p></td><td><p>3.46</p></td><td><p dir="rtl">I died in the middle</p></td></tr>

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<tr><td><p>0.03</p></td><td><p>9.74</p></td><td><p>0.02</p></td><td><p>0.02</p></td><td><p>0</p></td><td><p>0</p></td><td><p>10.2</p><p>4</p></td><td><p>12.8</p><p>9</p></td><td><p>0.0</p><p>4</p></td><td><p>0.07</p></td><td><p dir="rtl">Slavery F</p><p dir="rtl">Measurement</p><p dir="rtl">Y</p></td></tr><tr><td><p>0.12</p></td><td><p>0.16</p></td><td><p>0.01</p></td><td><p>0.02</p></td><td><p>0</p></td><td><p>0</p></td><td><p>0.18</p></td><td><p>0.35</p></td><td><p>0.0</p><p>1</p></td><td><p>0.02</p></td><td><p>CV</p></td></tr><tr><td><p>0.09</p></td><td><p>30.4</p><p>0</p></td><td><p>0.76</p></td><td><p>0.75</p></td><td><p>5</p></td><td><p>5</p></td><td><p>6.89</p></td><td><p>7.23</p></td><td><p>6.7</p><p>1</p></td><td><p>6.69</p></td><td><p dir="rtl">I died in the middle</p></td></tr><tr><td><p>0.02</p></td><td><p>16.2</p><p>2</p></td><td><p>0.19</p></td><td><p>0.13</p></td><td><p>0</p></td><td><p>0</p></td><td><p>2.74</p></td><td><p>1.27</p></td><td><p>1.9</p><p>2</p></td><td><p>1.48</p></td><td><p dir="rtl">Deviation of measurement</p></td></tr><tr><td><p>0.20</p></td><td><p>0.53</p></td><td><p>0.25</p></td><td><p>0.17</p></td><td><p>0</p></td><td><p>0</p></td><td><p>0.40</p></td><td><p>0.18</p></td><td><p>0.2</p><p>9</p></td><td><p>0.22</p></td><td><p>CV</p></td></tr><tr><td><p>0.09</p></td><td><p>31.2</p><p>1</p></td><td><p>0.65</p></td><td><p>0.54</p></td><td><p>5</p></td><td><p>5</p></td><td><p>9.35</p></td><td><p>6.70</p></td><td><p>8.4</p><p>6</p></td><td><p>11.2</p><p>7</p></td><td><p dir="rtl">I died in the middle</p></td></tr><tr><td><p>0.03</p></td><td><p>18.2</p><p>6</p></td><td><p>0.25</p></td><td><p>0.23</p></td><td><p>0</p></td><td><p>0</p></td><td><p>5.21</p></td><td><p>1.45</p></td><td><p>3.5</p><p>3</p></td><td><p>6.57</p></td><td><p dir="rtl">Deviation of measurement</p></td></tr><tr><td><p>0.30</p></td><td><p>0.59</p></td><td><p>0.38</p></td><td><p>0.44</p></td><td><p>0</p></td><td><p>0</p></td><td><p>0.56</p></td><td><p>0.22</p></td><td><p>0.4</p><p>2</p></td><td><p>0.58</p></td><td><p>CV</p></td></tr>

Example (9): Distinguishing silk coatings on polyester fabrics

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Analysis was performed by scanning electron microscope (SEM) using a Hitachi S-4800 filed emission SEM (FE-SEM) operated at an accelerating voltage of 2 kV. Pieces of each sample were cut using a razor blade and placed on carbon adhesive tape mounted on an aluminum SEM butt. An approximately 2 nm thick iridium coating was applied by sputter deposition in order to minimize 5 T surface charge buildup.

The samples used in the SEM study are described in Table 31. The exact SEM plots of the fabric samples are shown in Figures (105 - 167).

Table 31: Fabric samples tested by scanning electron microscope and measuring optical properties

<tr><td><p dir="rtl">Coating/treatment method using silk fibroin solution (sfs)</p></td><td><p dir="rtl">Silk solution for coating/curing (average molecular weight, Dalton)</p></td><td><p dir="rtl">Cloth</p></td><td><p dir="rtl">Sample identification code</p></td></tr><tr><td><p dir="rtl">Spray with 1% SFS</p></td><td><p>41.576</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-10-</p><p>SPRAY-B</p></td></tr><tr><td><p dir="rtl">Sprayed by 0.1% SFS</p></td><td><p>41.576</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-01-</p><p>SPRAY-B</p></td></tr><tr><td><p dir="rtl">Spray stencil with</p><p>sfs%1</p></td><td><p>41.576</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-10-</p><p>STEN-B</p></td></tr><tr><td><p dir="rtl">Bath with 1% sfs</p></td><td><p>41.576</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-10-</p><p>BATH-B</p></td></tr>

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<tr><td><p dir="rtl">bath by 0.1%</p><p>sfs</p></td><td><p>41.576</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-01-</p><p>BATH-B</p></td></tr><tr><td><p dir="rtl">Sprayed by 0.1% SFS</p></td><td><p>10.939</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-01-</p><p>SPRAY-C</p></td></tr><tr><td><p dir="rtl">Spray stencil with</p><p>sfs%0.1</p></td><td><p>10.939</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-01-</p><p>STEN-C</p></td></tr><tr><td><p dir="rtl">Bath with 1% sps</p></td><td><p>10.939</p></td><td><p dir="rtl">UA half</p><p dir="rtl">Finishing</p><p>R20904012</p></td><td><p>FAB-10-</p><p>BATH-C</p></td></tr>

The fabric SEM results showed that the silk solution was clearly deposited along and between the poly fabrics

Separate ester. Using a 0.1% silk solution produced less coating than an 8.0% silk solution. A bath of 0.1% silk solution having an average molecular weight of 41 kDa produced a uniform coating along the fiber with significant smooth features. Spraying with a 0.1% silk solution having an average molecular weight of 5 41 kDa produced the coating along the length of the fibers as well as a coating with a squeegee to bond the fibres.

Spraying with a 0.1% silk solution, having an average molecular weight of 11 kDa, produced a uniform coating with small dotted/polygonal features. Stencilling of a 0.1% silk solution, having an average molecular weight of 11 kDa, produced a coating along the length of the fiber that had clear edges and delineation between the coated and uncoated sides. Using a bath of 1.0% silk solution, having an average molecular weight of 41 kDa, 10 produced a thick coating along the fiber as well as a thick mop coating between the fibres. Use

A bath of 1.0% silk solution, having an average molecular weight of 11 kDa, produced a coating along all sides of the separated fibers. The paint appears uniform on the surface with numerous spot burrs. Use

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Spraying with a 1.0% silk solution, having an average molecular weight of 41 kDa, produced a coating along the length of the fiber as well as a squeegee coating interconnecting the fibers, which was thicker than observed with the 0.1% silk solution. Stencilling with a 1.0% silk solution, which has an average molecular weight of 41 kDa, produced a coating along and between the fibers, and the coating appeared well uniform.

<p dir="rtl">5 The SEM results showed that the silk coating was applied as a regular, thin, even coating on the fabric fibres. This amazing result demonstrates that the silk coating was applied to the fibers without the use of any additives or crosslinking using a water-based delivery system.</p>

Example (10): Marking silk coatings on polyester films

The membrane samples are mentioned in Table 32. SEM images of these membranes are shown in Figures 10 (168 - 237).

Table 32: Film samples tested by scanning electron microscope and measuring optical properties.

<tr><td><p dir="rtl">Coating/treatment method using silk fibroin solution (sfs)</p></td><td><p dir="rtl">Silk solution for coating/curing (average molecular weight, Dalton)</p></td><td><p dir="rtl">Polyester substrate material</p></td><td><p dir="rtl">Sample identification code</p></td></tr><tr><td><p dir="rtl">Spray with 1% SFS</p></td><td><p>41.576</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-10-</p><p>SPRAY-B-</p><p>01MYL</p></td></tr><tr><td><p dir="rtl">Sprayed by 0.1% SFS</p></td><td><p>41.576</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-01-</p><p>SPRAY-B-</p><p>01MYL</p></td></tr>

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<tr><td><p dir="rtl">Sprayed by 0.1% SFS</p></td><td><p>41.576</p></td><td><p>melinex 0.007</p></td><td><p>FIL-01-</p><p>SPRAY-B-</p><p>007MYL</p></td></tr><tr><td><p dir="rtl">Sprayed by 0.1% SFS</p></td><td><p>10.939</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-01-</p><p>SPRAY-C-</p><p>01MYL</p></td></tr><tr><td><p dir="rtl">Spray stencil with</p><p>sfs%0.1</p></td><td><p>41.576</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-01-</p><p>STEN-B-</p><p>01MYL</p></td></tr><tr><td><p dir="rtl">Spray stencil with</p><p>sfs%0.1</p></td><td><p>10.939</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-01-</p><p>STEN-C-</p><p>01MYL</p></td></tr><tr><td><p dir="rtl">Bath with 1% sfs</p></td><td><p>41.576</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-01-</p><p>BATH-B-</p><p>01MYL</p></td></tr><tr><td><p dir="rtl">Spray with 1% SFS</p></td><td><p>41.576</p></td><td><p>melinex 0.007</p></td><td><p>FIL-10-</p><p>BATH-B-</p><p>007MEL</p></td></tr><tr><td><p dir="rtl">Bath by 1 sfs</p></td><td><p>10.939</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-10-</p><p>BATH-C-</p><p>01MYL</p></td></tr>

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<tr><td><p dir="rtl">Bath by 0.1 sfs</p></td><td><p>41.576</p></td><td><p>Mylar 0.01</p></td><td><p>FIL-01-</p><p>BATH-B-</p><p>01MYL</p></td></tr>

The results show that the silk coatings were applied regularly. Small to no difference was observed in

10

15

20

Properties and morphology of polyester coated films. Surprisingly, the paint was even, regular, and thin. Furthermore, it was amazing that the silk was coated into the fibers without any additives or crosslinking using a water-based system.

Optical characterization was performed using a Zygo New View 6200 optical characterization meter. Two locations on each sample were randomly selected and measured at 10 mART magnification. The results are illustrated in Figures (241-264). The results indicate that the silk-coated samples had homogeneous deposition of silk fibroin. The surface roughness features observed in the comparison sample were visible after coating the silk on Mylar films, which was consistent with the presence of a thin silk film in the form of the coating. Bonded to Mylar film. The results demonstrate uniformity of the coating, and demonstrate that silk can be stenciled in discrete locations.

Contact properties were measured and cross-sectional samples were examined by SEM. The results are shown in Figures (265 - 268). For example, for sample -10-FIL SPRAY-B-10MYL, the thickness range is between approximately 260 and 580 nm in 4 locations analyzed. For sample FIL-10-BATH-B -01MYL, the thickness ranges between approximately 140 and 400 nanometers in 4 locations. SEM images in cross sections showed similar trends, with one site on sample FIL-10-SPRAY-B-10MYL having a cross section of approximately 500 nm and one on sample FIL-10-BATH-B-01MYL measuring approximately 180 nm.

Example (11): Preparation of silk fibroin solutions that have higher molecular weights

Preparation of fibroin solutions with higher molecular weights is given in Table 33.

Table 33: Preparation and properties of silk fibroin solutions

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<tr><td><p dir="rtl">Distraction</p><p dir="rtl">Multi</p><p dir="rtl">Average</p></td><td><p dir="rtl">Average molecular weight</p></td><td><p dir="rtl">Oven/solution temperature</p></td><td><p dir="rtl">Temperature degree</p><p>LiBr</p><p dir="rtl">)M(</p></td><td><p dir="rtl">Extraction temperature (35)</p></td><td><p dir="rtl">Extraction time (min)</p></td><td><p dir="rtl">Sample number</p></td></tr><tr><td><p>2.94</p></td><td><p>34.7</p></td><td><p dir="rtl">oven</p><p dir="rtl">100°C</p></td><td><p>100</p></td><td><p>100</p></td><td><p>60</p></td><td><p dir="rtl">group</p><p>A</p><p>TFF</p></td></tr><tr><td><p>3.17</p></td><td><p>44.7</p></td><td><p dir="rtl">oven</p><p dir="rtl">100°C</p></td><td><p>100</p></td><td><p>100</p></td><td><p>60</p></td><td><p dir="rtl">group</p><p>A DIS</p></td></tr><tr><td><p>3.07</p></td><td><p>41.6</p></td><td><p dir="rtl">100°C solution</p></td><td><p>100</p></td><td><p>100</p></td><td><p>60</p></td><td><p dir="rtl">group</p><p>B</p><p>TFF</p></td></tr><tr><td><p>3.12</p></td><td><p>44.0</p></td><td><p dir="rtl">100°C solution</p></td><td><p>100</p></td><td><p>100</p></td><td><p>60</p></td><td><p dir="rtl">group</p><p>B DIS</p></td></tr><tr><td><p>3.19</p></td><td><p>10.9</p></td><td><p dir="rtl">100°C solution</p></td><td><p>140</p></td><td><p>100</p></td><td><p>60</p></td><td><p dir="rtl">group</p><p>C</p><p>TFF</p></td></tr><tr><td></td><td></td><td><p dir="rtl">140°C solution</p></td><td><p>140</p></td><td><p>100</p></td><td><p>60</p></td><td><p dir="rtl">group</p><p>C DIS</p></td></tr><tr><td><p>2.56</p></td><td><p>129.7</p></td><td><p dir="rtl">140°C solution</p></td><td><p>60</p></td><td><p>90</p></td><td><p>30</p></td><td><p dir="rtl">group</p><p>D DIS</p></td></tr>

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<tr><td><p>2.73</p></td><td><p>144.2</p></td><td><p dir="rtl">60°C solution</p></td><td><p>60</p></td><td><p>90</p></td><td><p>30</p></td><td><p dir="rtl">group</p><p>D FIL</p></td></tr><tr><td><p>2.78</p></td><td><p>108.8</p></td><td><p dir="rtl">60°C solution</p></td><td><p>RT</p></td><td><p>100</p></td><td><p>15</p></td><td><p dir="rtl">group</p><p>E DIS</p></td></tr><tr><td><p>2.62</p></td><td><p>94.8</p></td><td><p dir="rtl">60°C solution</p></td><td><p>RT</p></td><td><p>100</p></td><td><p>15</p></td><td><p dir="rtl">group</p><p>E FIL</p></td></tr>

Example (12): Silk coatings on natural fibres

Coating natural fibers with silk fibroin solutions and their resulting properties are shown in Tables (34, 35) and Figures (269, 270). The results show that silk fibroin solutions can coat natural fibers Cotton - LYCRA includes LUON and POWER LUXTREME.

5 Table 34: Fibers coated with silk fibroin

<tr><td><p dir="rtl">Cloth</p></td><td><p dir="rtl">the key</p></td></tr><tr><td><p>Power Luxtreme RT 1211362</p></td><td><p>15072201</p></td></tr><tr><td><p>Luon RT 20602020</p></td><td><p>15072202</p></td></tr><tr><td><p dir="rtl">Spray paint with 1% silk solution on Power Luxtreme RT</p><p dir="rtl">1211362</p></td><td><p>15072301</p></td></tr><tr><td><p dir="rtl">1% silk solution spray paint on Luon RT20602020 (15072202)</p></td><td><p>15072302</p></td></tr><tr><td><p dir="rtl">Spray paint with 0.1% silk solution on Power Luxtreme</p><p>RT1211362 (15072201)</p></td><td><p>15072303</p></td></tr>

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<tr><td><p dir="rtl">Coating by spraying 0.1% silk solution on</p><p>Luon RT2O6O2O2O(15072202)</p></td><td><p>1507230)4</p></td></tr><tr><td><p dir="rtl">Stencil paint with 1% ink solution on</p><p>007] L Ireme RT1211362 (15072201)</p></td><td><p>15072305</p></td></tr><tr><td><p dir="rtl">Stencil paint with 1% ink solution on</p><p>Luon 1120602020 (15072202)</p></td><td><p>15072306</p></td></tr><tr><td><p dir="rtl">Stencil paint with 0.1% ink solution on</p><p>10] 11110 RT121 1362 (15722101)</p></td><td><p>1507230)7</p></td></tr><tr><td><p dir="rtl">001% stencil paint on</p><p>Luon 8120602020 (15072202)</p></td><td><p>15072308</p></td></tr><tr><td><p dir="rtl">Paint a bathroom with a 1% ink solution</p><p dir="rtl">٦501٤2٢٦) 21362 30٤٦ 90</p></td><td><p>15072309</p></td></tr><tr><td><p dir="rtl">Paint a bathroom with a 1% ink solution</p><p dir="rtl">1501222) 02060202</p></td><td><p>15072310</p></td></tr><tr><td><p dir="rtl">Bath paint with 0.1% silk solution on</p><p>Power Luxtrcmc RT1211362</p></td><td><p>15072311</p></td></tr><tr><td><p dir="rtl">Bath paint with 0.1% silk solution on</p><p>Luon RT2O6O2O20 (15072202)</p></td><td><p>150)72312</p></td></tr>

Table 35: Test results for fabrics coated with silk fibroin

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<tr><td><p dir="rtl">capacity</p><p dir="rtl">Control m</p></td><td><p dir="rtl">Factor</p><p dir="rtl">Transport</p></td><td><p dir="rtl">Internet sharing speed</p></td><td><p dir="rtl">Internet speed</p></td><td><p dir="rtl">n</p><p dir="rtl">P Q never</p><p dir="rtl">t</p></td><td><p dir="rtl">n</p><p dir="rtl">s</p><p dir="rtl">F</p><p dir="rtl">cat</p></td><td><p dir="rtl">an average</p><p dir="rtl">She died</p><p dir="rtl">s</p></td><td><p dir="rtl">an average</p><p dir="rtl">She died</p></td><td><p dir="rtl">Time to get wet</p></td><td><p dir="rtl">time</p></td><td><p dir="rtl">with me</p></td><td></td></tr><tr><td><p dir="rtl">Wetness</p></td><td></td><td></td><td><p dir="rtl">Char</p></td><td><p dir="rtl">wet</p></td><td></td><td></td><td><p dir="rtl">s</p></td><td></td><td><p dir="rtl">Get wet</p></td><td></td><td></td></tr><tr><td></td><td><p dir="rtl">single</p></td><td><p dir="rtl">in</p></td><td></td><td></td><td><p dir="rtl">R</p></td><td><p dir="rtl">a S</p></td><td></td><td><p dir="rtl">That's it</p></td><td></td><td><p dir="rtl">that</p></td><td></td></tr><tr><td><p dir="rtl">with it</p></td><td></td><td></td><td><p dir="rtl">in</p></td><td><p dir="rtl">unless</p></td><td></td><td></td><td><p dir="rtl">a S</p></td><td></td><td><p dir="rtl">the face</p></td><td></td><td></td></tr><tr><td></td><td><p dir="rtl">direction</p></td><td><p dir="rtl">That's it</p></td><td></td><td></td><td><p dir="rtl">the</p></td><td><p dir="rtl">That's it</p></td><td></td><td><p dir="rtl">^R</p></td><td></td><td><p dir="rtl">at</p></td><td></td></tr><tr><td><p dir="rtl">Al-Jam</p></td><td></td><td></td><td><p dir="rtl">the face</p></td><td><p dir="rtl">Q</p></td><td></td><td></td><td><p dir="rtl">the face</p></td><td></td><td><p dir="rtl">)second</p></td><td></td><td></td></tr><tr><td></td><td><p dir="rtl">I leave you</p></td><td><p dir="rtl">R</p></td><td></td><td></td><td><p dir="rtl">Season</p></td><td><p dir="rtl">R</p></td><td></td><td><p dir="rtl">)second</p></td><td></td><td><p dir="rtl">raw</p></td><td></td></tr><tr><td><p dir="rtl">mechanism</p></td><td></td><td></td><td><p dir="rtl">)millimeter/</p></td><td><p dir="rtl">s</p></td><td></td><td></td><td><p>/%(</p></td><td></td><td><p>)</p></td><td></td><td></td></tr><tr><td></td><td><p dir="rtl">(%)</p></td><td><p dir="rtl">)millimeter/</p></td><td></td><td></td><td><p dir="rtl">to</p></td><td><p>/%(</p></td><td></td><td><p>)</p></td><td></td><td></td><td></td></tr><tr><td><p>OM</p></td><td></td><td></td><td><p dir="rtl">second(</p></td><td><p dir="rtl">Yes</p></td><td></td><td></td><td><p dir="rtl">second(</p></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td><p dir="rtl">second(</p></td><td></td><td></td><td><p dir="rtl">)M</p></td><td><p dir="rtl">second(</p></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>MC</p></td><td></td><td></td><td></td><td><p dir="rtl">For the sake</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td><p dir="rtl">M(</p></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p dir="rtl">Cat</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p dir="rtl">)millimeter</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p>)</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>0.2</p></td><td><p>151.</p></td><td><p>1.4</p></td><td><p>0.1</p></td><td></td><td></td><td><p>8.6</p></td><td></td><td></td><td><p>64.</p></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>8.8</p></td><td><p>3.4</p></td><td></td><td></td><td></td></tr><tr><td><p>589</p></td><td><p>6524</p></td><td><p>168</p></td><td><p>503</p></td><td><p>5</p></td><td><p>5</p></td><td><p>049</p></td><td></td><td></td><td><p>378</p></td><td><p dir="rtl">^And</p></td><td><p>2201</p></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>123</p></td><td><p>072</p></td><td></td><td></td><td></td></tr><tr><td><p>8</p></td><td><p>8</p></td><td><p>6</p></td><td><p>8</p></td><td></td><td></td><td><p>4</p></td><td></td><td></td><td><p>6</p></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>28.</p></td><td><p>25.</p></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td><p>0.1</p></td><td><p>80.9</p></td><td><p>0.4</p></td><td><p>0.2</p></td><td></td><td></td><td><p>6.1</p></td><td><p>5.4</p></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p>5</p></td><td><p>5</p></td><td></td><td></td><td><p>192</p></td><td><p>178</p></td><td><p dir="rtl">^And</p></td><td><p>2202</p></td></tr><tr><td><p>529</p></td><td><p>572</p></td><td><p>244</p></td><td><p>18</p></td><td></td><td></td><td><p>95</p></td><td><p>636</p></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>2</p></td><td><p>6</p></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td><p>33.</p></td><td><p>21.</p></td><td><p>12.</p></td><td><p>10.</p></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td><p>0.2</p></td><td><p>143.</p></td><td><p>0.4</p></td><td><p>0.4</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p>5</p></td><td><p>5</p></td><td><p>619</p></td><td><p>985</p></td><td><p>280</p></td><td><p>717</p></td><td><p dir="rtl">^And</p></td><td><p>2301</p></td></tr><tr><td><p>808</p></td><td><p>6659</p></td><td><p>906</p></td><td><p>304</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td><p>6</p></td><td><p>9</p></td><td><p>4</p></td><td><p>2</p></td><td><p dir="rtl">Stroke</p></td><td></td></tr>

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<tr><td><p>0.1</p></td><td></td><td></td><td><p>0.2</p></td><td></td><td></td><td><p>8.7</p></td><td><p>6.1</p></td><td><p>41.</p></td><td><p>25.</p></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td></td><td><p>44.0</p></td><td><p>0.1</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>070</p></td><td></td><td></td><td><p>333</p></td><td><p>5</p></td><td><p>5</p></td><td><p>028</p></td><td><p>651</p></td><td><p>502</p></td><td><p>889</p></td><td rowspan="2"><p dir="rtl">^And</p></td><td><p>2302</p></td></tr><tr><td></td><td><p>6124</p></td><td><p>791</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>4</p></td><td></td><td></td><td><p>6</p></td><td></td><td></td><td><p>2</p></td><td><p>2</p></td><td><p>6</p></td><td><p>8</p></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td><p>19.</p></td><td></td><td></td><td></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td><p>0.5</p></td><td><p>370.</p></td><td><p>1.3</p></td><td><p>0.3</p></td><td></td><td></td><td></td><td><p>7.9</p></td><td><p>4.7</p></td><td><p>42.</p></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p>5</p></td><td><p>4</p></td><td><p>372</p></td><td></td><td></td><td></td><td rowspan="2"><p dir="rtl">^And</p></td><td><p>2303</p></td></tr><tr><td><p>297</p></td><td><p>2757</p></td><td><p>64</p></td><td><p>261</p></td><td></td><td></td><td></td><td><p>114</p></td><td><p>268</p></td><td><p>152</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td><p>5</p></td><td></td><td></td><td></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td><p>0.1</p></td><td></td><td><p>0.3</p></td><td></td><td></td><td></td><td><p>6.6</p></td><td><p>5.0</p></td><td><p>34.</p></td><td><p>78.</p></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td></td><td><p>94.9</p></td><td></td><td><p>0.0</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>684</p></td><td></td><td><p>872</p></td><td></td><td><p>5</p></td><td><p>5</p></td><td><p>321</p></td><td><p>148</p></td><td><p>313</p></td><td><p>474</p></td><td rowspan="2"><p dir="rtl">^And</p></td><td><p>2304</p></td></tr><tr><td></td><td><p>7976</p></td><td></td><td><p>661</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>8</p></td><td></td><td><p>8</p></td><td></td><td></td><td></td><td><p>2</p></td><td><p>6</p></td><td><p>8</p></td><td><p>6</p></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td><p>0.2</p></td><td><p>139.</p></td><td><p>0.8</p></td><td><p>0.1</p></td><td></td><td></td><td><p>6.2</p></td><td><p>6.2</p></td><td><p>17.</p></td><td><p>36.</p></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td><p>305</p></td><td><p>7347</p></td><td><p>904</p></td><td><p>887</p></td><td><p>5</p></td><td><p>5</p></td><td><p>552</p></td><td><p>715</p></td><td><p>203</p></td><td><p>195</p></td><td><p dir="rtl">^And</p></td><td><p>2305</p></td></tr><tr><td><p>2</p></td><td><p>8</p></td><td><p>6</p></td><td><p>2</p></td><td></td><td></td><td><p>6</p></td><td><p>8</p></td><td><p>8</p></td><td><p>4</p></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td><p>0.1</p></td><td></td><td><p>0.3</p></td><td></td><td></td><td></td><td><p>6.6</p></td><td><p>5.0</p></td><td><p>34.</p></td><td><p>78.</p></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td></td><td><p>94.9</p></td><td></td><td><p>0.0</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>684</p></td><td></td><td><p>872</p></td><td></td><td><p>5</p></td><td><p>5</p></td><td><p>321</p></td><td><p>148</p></td><td><p>313</p></td><td><p>474</p></td><td rowspan="2"><p dir="rtl">^And</p></td><td><p>2306</p></td></tr><tr><td></td><td><p>7976</p></td><td></td><td><p>661</p></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td><p>8</p></td><td></td><td><p>8</p></td><td></td><td></td><td></td><td><p>2</p></td><td><p>6</p></td><td><p>8</p></td><td><p>6</p></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>17.</p></td><td></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td><p>0.2</p></td><td><p>139.</p></td><td><p>0.8</p></td><td><p>0.1</p></td><td></td><td></td><td><p>6.2</p></td><td><p>6.2</p></td><td></td><td><p>36.</p></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p>5</p></td><td><p>5</p></td><td></td><td></td><td><p>203</p></td><td></td><td rowspan="2"><p dir="rtl">^And</p></td><td><p>2307</p></td></tr><tr><td><p>305</p></td><td><p>7348</p></td><td><p>905</p></td><td><p>887</p></td><td></td><td></td><td><p>553</p></td><td><p>716</p></td><td></td><td><p>195</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>8</p></td><td></td><td><p dir="rtl">Stroke</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>25.</p></td><td></td><td><p dir="rtl">died</p></td><td><p>1507</p></td></tr><tr><td><p>0.1</p></td><td><p>117.</p></td><td><p>0.6</p></td><td><p>0.1</p></td><td></td><td></td><td><p>6.4</p></td><td><p>5.6</p></td><td></td><td><p>57.</p></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td><p>5</p></td><td><p>5</p></td><td></td><td></td><td><p>758</p></td><td></td><td rowspan="2"><p dir="rtl">^And</p></td><td><p>2308</p></td></tr><tr><td><p>995</p></td><td><p>3573</p></td><td><p>389</p></td><td><p>274</p></td><td></td><td></td><td><p>437</p></td><td><p>432</p></td><td></td><td><p>335</p></td><td></td></tr><tr><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td><p>8</p></td><td></td><td><p dir="rtl">Stroke</p></td><td></td></tr>

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<tr><td><p>0.3</p><p>672</p><p>4</p></td><td><p>267.</p><p>0755</p></td><td><p>0.8</p><p>530</p><p>6</p></td><td><p>0.0</p><p>939</p><p>8</p></td><td><p>5</p></td><td><p>5</p></td><td><p>9.1</p><p>106</p><p>4</p></td><td><p>4.0</p><p>159</p><p>4</p></td><td><p>9.2</p><p>662</p></td><td><p>54.</p><p>138</p><p>4</p></td><td><p dir="rtl">died</p><p dir="rtl">^And</p><p dir="rtl">Stroke</p></td><td><p>1507</p><p>2309</p></td></tr><tr><td><p>0.1</p><p>794</p></td><td><p>104.</p><p>5035</p></td><td><p>0.5</p><p>111</p></td><td><p>0.3</p><p>059</p></td><td><p>5</p></td><td><p>5</p></td><td><p>7.8</p><p>956</p></td><td><p>6.8</p><p>844</p></td><td><p>13.</p><p>665</p><p>8</p></td><td><p>28.</p><p>454</p><p>4</p></td><td><p dir="rtl">died</p><p dir="rtl">^And</p><p dir="rtl">Stroke</p></td><td><p>1507</p><p>2310</p></td></tr><tr><td><p>0.3</p><p>597</p></td><td><p>246.</p><p>6729</p></td><td><p>1.1</p><p>702</p></td><td><p>0.9</p><p>486</p></td><td><p>5</p></td><td><p>5</p></td><td><p>13.</p><p>027</p><p>7</p></td><td><p>8.8</p><p>047</p></td><td><p>4.4</p><p>738</p></td><td><p>5.1</p><p>292</p></td><td><p dir="rtl">died</p><p dir="rtl">^And</p><p dir="rtl">Stroke</p></td><td><p>1507</p><p>2311</p></td></tr><tr><td><p>0.1</p><p>461</p></td><td><p>73.4</p><p>005</p></td><td><p>0.5</p><p>794</p></td><td><p>0.7</p><p>394</p></td><td><p>5</p></td><td><p>5</p></td><td><p>11.</p><p>726</p><p>8</p></td><td><p>11.</p><p>068</p><p>4</p></td><td><p>9.4</p><p>722</p></td><td><p>6.8</p><p>516</p></td><td><p dir="rtl">died</p><p dir="rtl">^And</p><p dir="rtl">Stroke</p></td><td><p>1507</p><p>2312</p></td></tr>

All patents, patent applications, and published references cited herein are included in their entirety.

In this application for reference. While the methods of the present invention have been described in connection with specific embodiments thereof, it is understood that they may be modified. This application is also intended to cover any variations, uses, or adaptations of the methods of the present invention, including those variations from the present invention that are within known or usual practice in the field to which the methods of the present invention pertain.

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