Nonpeptidyl compounds and pharmaceutical compositions

Description of the invention

The invention relates to non-peptidyl interleukin-1b converting enzyme ("ICE") inhibitors and a pharmaceutical composition. The compounds according to the invention are characterized by particular structural and physicochemical characteristics. The compounds and pharmaceutical composition of the invention are particularly suitable for inhibiting the activity of ICE and consequently can be advantageously used as agents against interleukin-1 (IL-1) dependent diseases, including inflammatory, autoimmune and neurodegenerative diseases.

Interleukin-1 (IL-1) is the major proinflammatory and immunoregulatory protein that stimulates the differentiation and proliferation of fibroblasts, production of prostaglandins, collagenase and phospholipase by synovial cells and chondrocytes, degranulation of basophils and eosinophils, and activation of neutrophilic cells. JH Oppenheim et al. Immunology Today, 7, pp. 45-56 (1986). As such, it is therefore associated with the pathogenesis of chronic and acute inflammation and autoimmune diseases. IL-1 is produced mainly by peripheral blood monocytes as part of the inflammatory response and exists in two distinct agonist forms, IL-1a and IL-1b. BS Mosely et al., Proc. Nat. Acad. Sci. 84, pp. 4572-4576 (1987); G. Lonnemann et al., Eur. J. Immunol., 19, pp. 1531-1536 (1989).

IL-1b is synthesized as a biologically inactive precursor, pIL-1b. pIL-1b does not have a conventional leader sequence and is not processed by a signal peptidase. CJ March, Nature, 315, pp. 641-647 (1985). In contrast, pIL-1b is cleaved by interleukin-1b converting enzyme (ICE) between Asp-116 and Ala-117 to form a biologically active C-terminal fragment present in human serum and synovial fluid. PR Sleat et al., J. Biol. Chem., 265, pp. 14526-14528 (1992); AD Howard et al., J. Immunol., 147 pp. 2964-2969 (1991). Treatment with ICE is also necessary for the transport of parental IL-1b across the cell membrane.

ICE is a cysteine ​​protease mainly located in monocytes. It transforms IL-1b into a mature form. RA Black et al., FEBS Lett., 247, pp. 386-390 (1989); MJ Kostura et al., Proc. Natl. Acad. Sci. USA, 86, pp. 6227-5231 (1989). ICE, or its homologs, are also involved in regulating cell death or apotosis. J. Yuan et al., Cell, 75, pp. 641-652 (1993); M. Miura et al., Cell, 75, pp. 653-660 (1993); MA Nett-Fiordalisi et al., J. Cell Biochem., 17B, p. 117 (1993). In particular, ICE or ICE homologues are believed to be associated with the regulation of apoptosis in neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. J. Marx and M. Baringa, Science, 259, pp. 760-762 (1993); V. Gagliardini et al., Science, 263, pp. 826-828 (1994).

ICE has previously been described as a heterodimer composed of two subunits, p20 and p10 (20 kDa and 10 kDa molecular weight, respectively). These subunits are derived from the 45 kDa proenzyme (p45) in the form of p30, through an activation mechanism that is autocatalytic. NA Thornberry et al, Nature, 356, pp. 768-774 (1992). The ICE proenzyme has been divided into several functional domains: the prodomain (p14), the p22 / 20 subunit, the polypeptide linker and the p10 subunit. Thornberry et al., Supra; Casano et al., Genomics, 20, pp. 474-481 (1994).

The entire length of p45 was characterized by cDNA and amino acid sequences. PCT patent applications WO 91/15577 and WO 94/00154. cDNA and the amino acid sequences of p20 and p10 are also known. Thornberry et al., Supra. Mouse and rat ICE have also been sequenced and cloned. They show a high degree of amino acid and nucleic acid sequence homology with human ICE. DK Miller et al., Ann. NY Acad. Sci., 696, pp. 133-148 (1993); SM Molineaux, Proc. Nat. Acad. Sci., 90, pp. 1809-18813 (1993). However, the knowledge of the primary structure of ICE makes it impossible to predict its tertiary structure. This does not provide knowledge of the structural, conformational and chemical interactions of ICE and its substrate pIL-1b or other substrates or inhibitors.

ICE inhibitors are a class of compounds useful in combating inflammation or apoptosis, or both. Peptide and peptidyl ICE inhibitors have been described. PCT patent applications WO91 / 15577; WO 93/05071; WO 93/09135; WO 93/14777 and WO 93/16710; and European patent application 0547 699.

In the publication of NA Thornberry et al., Inactivation of Interleukin-1b Converting Enzyme by Peptide (Acyloxy) methyl Ketones in Biochemistry, 33 pp. 3934-3940, ICE inhibitors of the type Ac-Tyr-Val-Ala-Asp-CH are presented.₂-O-CO-aryl.

In RE Dolle et al., P1 Aspartate-Based Peptide a- (2,6-Dichlorobenzoyl) oxy) methyl Ketones as Potent Time-Dependent Inhibitors of Interleukin-1b Converting Enzyme, J. Med. Chem. 37,

No. 193 391 B1 pp. 563-564 (1994), non-cyclic amino acid and peptide derivatives are described in which the N-terminal amino group is protected as a carbobenzyloxy derivative. These are ICE inhibitors similar to those described in Thornberry and like them also contain an acyloxymethylketone group.

European patent EP 0519748 describes non-cyclic ICE inhibitors of the type X-Try-Val-Asp-CH2-O-CO-aryl.

However, due to their peptide nature, such inhibitors usually have undesirable pharmacological properties, such as poor oral absorption, poor stability and rapid metabolism. JJ Plattner and DW Norbeck, in Drug Discovery Technologies, CR Clark and WH Moos, eds. (Ellis Horwood, Chichester, England, 1990), pp. 92-126. This is an obstacle to their use as effective drugs.

Accordingly, there is a need for compounds that can effectively inhibit the action of ICE and are useful as agents for the prevention and treatment of chronic and acute forms of IL-1 related diseases, including a variety of cancers, as well as inflammation, autoimmune and neurodegenerative diseases. .

The present invention provides a new class of compounds and pharmaceutically acceptable derivatives thereof that are useful as ICE inhibitors. These compounds can be used alone or in combination with other therapeutic and prophylactic agents, such as antibiotics, immunomodulators, or other anti-inflammatory agents, for the treatment or prevention of IL-1 dependent diseases. The compounds of the invention are capable of binding to the active site of ICE and inhibiting the activity of this enzyme.

The description uses the following terms:

The term active site means any or all of the following sites in ICE: substrate binding site, inhibitor binding site and site where cleavage of the substrate occurs. The active center is characterized by at least the following amino acid residues: 173, 176, 177, 178, 179, 180, 236, 237, 238, 239, 244, 248, 283, 284, 285, 290, 338, 339, 340, 341, 342 , 343, 344, 345, 348, 352, 381,383 (sequence and numbering according to Thornberry et al., Supra).

The terms binding sub-site, S sub-site, S-cavity, etc., mean binding sub-sites or portions of a substrate binding site in an ICE molecule. The amino acid residues of the substrate are determined according to their position relative to the broken bond, ie the bond that is broken by the protease. Residues toward the N-terminus of the substrate are designated P1, P2, etc., and residues toward the C-terminus of the substrate are designated P1 ', P2', etc. Parts of the inhibitor that correspond to the P or P 'residues of the substrate are also designated P1 and P1', etc., by analogy to the substrate. Binding sub-sites in an ICE molecule that take up residues labeled P1, P1 ', etc. are labeled S1, S1' etc., or alternatively may be labeled P1 binding cavity, P1 'binding cavity, etc. [I. Schechter and A. Berger, On the Size of the Active Site in Proteases, Biochem. Biophys, Res. Commun.vol. 25, pp. 157-162 (1967)].

The terms P2 binding cavity or S2 sub-site in the active center of ICE are synonymous and are defined as the space encompassed by the amino acid residues Pro-290, Val-338 and Trp-340.

The terms P3-binding cavity or S3 sub-site in the active center of ICE are synonymous and are defined as the space encompassed by the amino acid residues Pro-177, Arg-178, Thr-180, Arg-341, or Pro-343.

The terms P4 binding cavity or S4 sub-site in the active center of ICE are synonymous and are defined as the space encompassed by the amino acid residues His-342, Met-345, Val-348, Arg-352, Asp-381, Arg-383 or Trp-340.

The terms P1-binding cavity or S1 sub-site in the active center of ICE are synonymous and are defined as the space encompassed by amino acid residues Arg-179, His-237, Gln-383, or Arg-341.

The terms P 'binding cavity or S' sub-site in the active center of ICE are synonymous and are defined as the space encompassed by the amino acid residues Phe-173, Ile-176, His-237, Gly-238, Ile-239, Cys-244 or His- 248.

The term hydrophobic refers to a residue that is non-water-soluble and fat-soluble. Hydrophobic residues include, but are not limited to, hydrocarbons such as alkanes, alkenes, alkynes, cycloalkanes, cycloalkenes, cycloalkines, and aromatics such as aryls, certain saturated and unsaturated heterocycles, and residues that are substantially similar to the hydrophobic side chains of natural and unnatural α-amino acids, including valine, leucine, isoleucine, methionine, phenylalanine, α-aminoisobutyric acid, alloisoleucine, tyrosine, and tryptophan.

PL 193 391 B1

The term moderately hydrophobic refers to a hydrophobic residue in which one or two carbon atoms have been replaced by more polar atoms such as oxygen or nitrogen atoms.

The term association is used to refer to a state of proximity between an inhibitor or portions thereof and an ICE molecule or portions thereof, the juxtaposition being energetically favored by electrostatic or van der Waals interactions.

The term hydrogen bonding refers to the beneficial interaction that occurs when the respective donor atom, X, containing a proton, H, and the corresponding acceptor atom, Y, are within a distance of

0.25nm -0.35nm, and the angle X-H ------- Y is greater than 90 degrees. Suitable donor and acceptor atoms are well known in medical chemistry (GC Pimentel and AL McClellan, The Hydrogen Bond, Freeman, San Francisco, 1960; R. Taylor and O. Kennard, Hydrogen Bond Geometry and Organic Crystals, Account of Chemical Research, 17 , pp. 320-236 (1984)).

The term salt bridge refers to a non-covalent attraction interaction between a positively charged residue (P) and a negatively charged residue (N) when between the mass centers of P and N is in the range of 0.2 nm and 0.6 nm . When calculating the center of mass, the atoms that carry a formal charge and the atoms immediately adjacent to them are taken into account. For example, an electrolytic bridge may be formed between a positively charged guanidinium side chain in the arginine residue and a negatively charged carboxylate side chain in the glutamate residue. Electrolytic bridges are well known in medical chemistry (L. Stryer, Biochemistry, Freeman, San Francisco, (1975); KA Dill, Dominant Forces in Protein Folding, Biochemistry, 29, No. 31, pp. 7133-7155 (1990)).

The term center of mass refers to a point in three-dimensional space that represents the weighted average position of the component masses.

The symbol Ki refers to the numerical measurement of a compound's effectiveness in inhibiting the activity of a target enzyme, such as ICE. Lower Ki values ​​mean higher efficiency. The Ki value is derived by fitting the experimentally determined rate values ​​to a standard enzyme kinetic equation (see IH Segel, Enzyme Kinetics, Wiley-Interscience, 1975).

The term minimize refers to a systematic change in the spatial arrangement of atoms in a molecule or molecular complex such that any further minor perturbations in the geometry of the atoms would increase the total energy of the system as measured by molecular force-field mechanisms. Minimization and molecular force-field mechanisms are well known in computer chemistry [U. Burkert and NL Allinger, Molecular Mechanics, ACS Monograph 177, America Chemical Society, Washington, 1982, pp. 59-78].

The term strain energy is used in this application to refer to the difference between the free conformation energy and the bound conformation energy of an ICE. Stress energy can be determined by performing the following steps: Energy evaluation a molecule that has the conformation needed to bind to ICE. Then minimize and re-evaluate the energy which is the conformational energy of the free compound. The stress energy for binding a potential inhibitor to ICE is the difference between the conformational energy of the free compound and the conformational energy of the bound compound. In a preferred embodiment, the stress energy of an inhibitor of the invention is less than about 0.0418 kJ / mol.

The term patient as used herein refers to any mammal, especially humans.

The term pharmaceutically effective amount means an amount effective to treat or ameliorate IL-1 related diseases in a patient. The term prophylactically effective amount means an amount effective to prevent or substantially reduce the symptoms of an IL-1 related disease in a subject.

The term pharmaceutically acceptable carrier or excipient refers to a non-toxic carrier or excipient that can be administered to a patient together with a compound of the invention without abolishing its pharmacological activity.

The term pharmaceutically acceptable derivative means any pharmaceutically acceptable salt, ester, or salt of such ester of a compound of the invention, or any other compound which, when administered to a patient, is capable of providing (directly or indirectly) a compound of the invention or a metabolite with anti-ICE or anti-ICE activity. his rest.

Pharmaceutically acceptable salts of the compounds of the invention include, for example, salts formed with pharmaceutically acceptable inorganic and organic acids and bases. Examples of suitable acids include hydrochloric, hydrobromic, sulfuric, nitric, perchloric, fumaric, maleic, phosphoric, glycolic, lactic, salicylic, succinic, toluene-p-sulfonic, tartaric, acetic, citric, methanesulfonic, formic, benzoic, malonic, naphthalene -2-sulfonic and benzenesulfonic. Other acids, such as oxalic, while not themselves pharmaceutically acceptable, can be used in the preparation of salts useful as intermediates in the preparation of the compounds of the invention and their pharmaceutically acceptable acid addition salts. Salts formed with suitable bases include alkali metal (e.g. sodium), alkaline earth metal (e.g. magnesium), ammonium and N- (C1-C4alkyl) salts 4⁺.

The invention also envisages the quaternization of any basic nitrogen containing groups in the compounds of the invention. Basic nitrogen can be quaternized by means known to those skilled in the art including, for example, lower alkyl halides such as methyl, ethyl, propyl, and butyl chloride, bromides and iodides; dialkyl sulfates including dimethyl, diethyl, dibutyl and diamyl sulfate; long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides; and aralkyl halides, including benzyl and phenethyl bromides. By such quaternization, water- or oil-soluble or dispersible products can be obtained.

The ICE inhibitors of the invention may contain one or more asymmetric carbon atoms and thus may exist as racemates or racemic mixtures, single enantiomers, distereomeric mixtures and single diastereomers. All isomeric forms of the compounds of the invention are within the scope of the invention. Each stereogenic carbon atom may be in the R or S configuration. While specific compounds exemplified in this application may be represented in a particular stereochemical configuration, compounds and mixtures thereof having opposite stereochemistry at a given chiral center are also contemplated.

The ICE inhibitors of the invention may include ring structures that may be optionally substituted on carbon, nitrogen or other atoms with a variety of substituents. Such ring structures can be singly or multiply substituted. Preferably, the ring structures contain from 0 to 3 substituents. For multiple substitution, each substituent may be selected independently of the other substituents as long as the combination thereof results in the formation of a stable compound.

Only combinations of substituents and variables that result in the formation of a stable compound are included in the invention. The term stable as used herein refers to compounds which are stable enough to be produced and administered to mammals by methods known in the art. Typically, such compounds are stable for at least one week at temperatures of 40 ° C or less in the absence of moisture or other chemically reactive conditions.

Many of the conventional techniques can be used to evaluate the enzyme inhibitory activity of individual compounds as well as to evaluate a candidate compound for screening for inhibition of ICE activity. Generally, such techniques include determining the distribution and bond proximity of a given residue, the space occupied of the bonded inhibitor, the bond deformation energy of a given compound, and electrostatic interaction energy. Examples of conventional techniques useful for the above research include: quantum mechanics, molecular mechanics, molecular dynamics, Monte Carlo method, systems research method and spatial geometry. (GR Marshall, Ann. Ref. Pharmacol. Toxicol., 27, p. 193 (1987)). Specific computer software has also been developed for use in such methods. Examples of computer programs for such applications include: Gaussian 92, revision E.2 (MJ Frisch, Gaussian, Inc., Pittsburgh, PA © 1993); AMBER, version 4.0 (PA Kollman, University of California, San Francisco, © 1993); QUANTA / CHARMM [Molecular Simulation, Inc. Burlington, MA © 1992); and Insight II / Discover (Biosysm Technologies Inc., San Diego, CA © 1992). These programs can be implemented, for example, on a Silicon Graphics Indigo 2 or IBM RISC / 6000 model 550 workstation. Other computer systems and software packages will be known and will be readily employed by those skilled in the art.

The various active ICE inhibitors of the invention may similarly interact with different binding cavities at the active center of ICE. The spatial distribution of these important groups is often referred to as a pharmacophore. The pharmacophore concept has been thoroughly described in the literature (D. Mayer, CB Naylor, I. Motoc and GR Marshall, J. Comp. Aided Molec. Design, vol. 1,

PL 193 391 B1 pp 3-16 (1987); A. Hopfinger and BJ Burke, in Concepts and Applications of Molecular Similarity, MA Johnson and GM Maggiora, eds., Wiley (1990)).

Different scaffolds or core structures may be used in the various ICE inhibitors of the invention, but in all such cores the necessary residues will be placed at the active site so that the specific interactions necessary for binding take place. These compounds are best defined in terms of their ability to conform to the pharmacophore, i.e. their structural similarity to the shape and nature of the ICE active site.

Non-peptidyl interleukin-1b converting enzyme inhibitors of the invention are represented by the formula:

and CH₂-WHAT₂-R /

Ri-NH-CH \

wherein:

R1 is selected from the groups with the following formulas:

<img file="PL193391B1_D0001.tif" />

R is H or straight or branched C1-6alkyl,

R3 is -C (O) H, -C (= NN (H) -C (O) -NH2) H, -C (O) CH2OC (O) Ar1, where Ar1 is 2,6-dichlorophenyl or

<img file="PL193391B1_D0002.tif" />

In the R1 groups: R20 is

<img file="PL193391B1_D0003.tif" />

optionally substituted with phenoxy or 1,3-diothiolane,

Q1 is H or linear or branched C1-6alkyl substituted with phenyl, wherein the phenyl is optionally substituted with -OH, linear or branched -OC1-6alkyl, or -CF3,

R5 is H or straight or branched C1-6alkyl substituted with phenyl,

R6 is H or straight or branched C1-6alkyl;

or R1 is a group of the formula:

<img file="PL193391B1_D0004.tif" />

R is H or straight or branched C1-6 alkyl,

R3 means:

-C (O) H,

-C (O) CH2OH,

-C (O) CH2CH3,

-C (O) CH2OC (O) Ar1, where Ar1 is 2,6-dichlorophenyl, -C (O) CH2OCH2Ar1, where Ar1 is 2-chlorophenyl, -C (O) CH2SCH2Ar1, where Ar1 is 2-chlorophenyl,

<img file="PL193391B1_D0005.tif" />

and in the group R1 of formula (c)

R5 is -COR9, -CO-O-R9, -SO-R9 or AcTyr, wherein R9 is straight or branched C1-6alkyl substituted with phenyl,

R6 is H, or straight or branched C1-6alkyl,

PL 193 391 B1

Q1 is H, phenyl, or straight or branched C1-6alkyl optionally substituted with phenyl; or R1 is a group of the formula:

<img file="PL193391B1_D0006.tif" />

wherein Q1 is straight or branched C1-6alkyl; R is H; and R3 is -C (O) H; or R1 is:

<img file="PL193391B1_D0007.tif" />

R5 means:

-C (O) R9; where R9 is straight or branched C1-6alkyl optionally substituted with phenyl, or

-SO2-R9, where R9 is straight or branched C1-6alkyl and R is H or straight or branched C1-6alkyl,

R3 means:

-C (O) H,

-C (O) CH2SCH2Ar1, where Ar1 is 2-chlorophenyl,

-C (O) CH2OCH2Ar1, where Ar1 is 2-chlorophenyl,

-C (O) CH2OC (O) Ar1, where Ar1 is 2,6-dichlorophenyl,

<img file="PL193391B1_D0008.tif" />

Or R1 is a group of the formula:

<img file="PL193391B1_D0009.tif" />

wherein:

R5 is -C (O) R9, where R9 is a straight or branched C1-6alkyl group which is substituted with phenyl, and

R is H,

R3 is -C (O) H;

or R1 is a group of the formula:

<img file="PL193391B1_D0010.tif" />

<img file="PL193391B1_D0011.tif" />

wherein:

R5 is -COR9, wherein R9 is straight or branched C1-6alkyl, each R6 independently is straight or branched C1-6alkyl, optionally substituted with

4-hydroxyphenyl, a

PL 193 391 B1

Q1 is -O-phenyl or -OR9, wherein R9 is straight or branched C1-6alkyl which is substituted with phenyl or unsaturated,

R is H or straight or branched C1-6alkyl,

R3 means:

-C (O) H,

-C = NN (H) -C (O) -NH2) H,

-C (O) CH2SCH2Ar1, where Ar1 is 2-chlorophenyl,

<img file="PL193391B1_D0012.tif" />

or R1 is a group of the formula:

<img file="PL193391B1_D0013.tif" />

where R20 is:

<img file="PL193391B1_D0014.tif" />

R is H,

R3 is -C (O) H, or R1 is:

<img file="PL193391B1_D0015.tif" />

PL 193 391 B1 in which:

each R5 independently means:

-C (O) R9 where R9 is straight or branched C1-6alkyl substituted with phenyl or 4-hydroxyphenyl, or

-C (O) OR9; wherein R9 is straight or branched C1-6alkyl substituted with phenyl,

Q1 is -O-phenyl or -OR9 wherein R9 is straight or branched C1-6alkyl and R6 is H or straight or branched C1-6alkyl;

R is H,

R3 is -C (O) H, or R1 is a group of formula:

<img file="PL193391B1_D0016.tif" />

where R5 is:

-C (O) Ar1, where Ar1 is phenyl, or

-C (O) R9, where R9 is straight or branched C1-6alkyl substituted with phenyl; R is H or straight or branched C1-6alkyl,

R3 means:

-C (O) H,

-C (= NN (H) -C (O) -NH2) H,

-C (O) CH2OC (O) Ar1, where Ar1 is 2,6-dichlorophenyl,

<img file="PL193391B1_D0017.tif" />

or R1 is a group of the formula:

<img file="PL193391B1_D0018.tif" />

where R5 is:

-C (O) R9 where R9 is straight or branched C1-6alkyl optionally substituted with phenyl;

R7 means:

-H,

-R9, where R9 is straight or branched C1-6alkyl optionally substituted with phenyl, -C (O) R9 where R9 is straight or branched C1-6alkyl optionally substituted with phenyl:

PL 193 391 B1

R is H or straight or branched C1-6alkyl,

R3 means:

-C (O) H,

-C (O) CH2OH,

-C (O) CH2CH3,

-C (O) CH2OC (O) Ar1, where Ar1 is 2,6-dichlorophenyl, -C (= NO-CH2Ar1) H, where Ar1 is 2,6-dichlorophenyl,

<img file="PL193391B1_D0019.tif" />

Preferably R1 is a group of formula:

<img file="PL193391B1_D0020.tif" />

<img file="PL193391B1_D0021.tif" />

PL 193 391 B1

Also preferred is the group R 1 of the formula:

<img file="PL193391B1_D0022.tif" />

Further preferred R 1 groups are represented by the following formulas:

<img file="PL193391B1_D0023.tif" />

<img file="PL193391B1_D0024.tif" />

PL 193 391 B1

<img file="PL193391B1_D0025.tif" />

PL 193 391 B1

A preferred compound of the invention wherein R1 is a group of formula (a1), (a2), (a3) ​​or (g2) is selected from the group consisting of:

<img file="PL193391B1_D0026.tif" />

PL 193 391 B1

<img file="PL193391B1_D0027.tif" />

from the group consisting of:

<img file="PL193391B1_D0028.tif" />

<img file="PL193391B1_D0029.tif" />

PL 193 391 B1

<img file="PL193391B1_D0030.tif" />

<img file="PL193391B1_D0031.tif" />

PL 193 391 B1

<img file="PL193391B1_D0032.tif" />

<img file="PL193391B1_D0033.tif" />

<img file="PL193391B1_D0034.tif" />

PL 193 391 B1

<img file="PL193391B1_D0035.tif" />

<img file="PL193391B1_D0036.tif" />

PL 193 391 B1

A preferred compound of the invention wherein R1 is a group of formula (d2) is a compound of the following formula:

<img file="PL193391B1_D0037.tif" />

The group of compounds shown below comprises the preferred compounds according to the invention, wherein R 1 is a group of formula (e1) or (e2):

<img file="PL193391B1_D0038.tif" />

PL 193 391 B1

<img file="PL193391B1_D0039.tif" />

Preferred compounds of the invention in which R 1 is a group of formula (e4) or (e7) have the following formulas:

<img file="PL193391B1_D0040.tif" />

PL 193 391 B1

However, a preferred compound in which Ri is a group of formula (f), (f2), (f3), (f4) or (f5) is selected from the group consisting of:

<img file="PL193391B1_D0041.tif" />

The following are also preferred compounds in which R 1 is a group of formula (ai), (a2), (a3) ​​or (g2):

<img file="PL193391B1_D0042.tif" />

PL 193 391 B1

<img file="PL193391B1_D0043.tif" />

A preferred compound of the invention wherein R 1 is a group of formula (o1-a) or (o6-a) is selected from the group consisting of:

PL 193 391 B1

<img file="PL193391B1_D0044.tif" />

Compound 39 is an example of a preferred compound of the invention wherein R1 is a group of formula (h2):

<img file="PL193391B1_D0045.tif" />

PL 193 391 B1

<img file="PL193391B1_D0046.tif" />

However, a preferred compound in which R1 is a group of formula (w1) is selected from the group consisting of:

<img file="PL193391B1_D0047.tif" />

PL 193 391 B1

<img file="PL193391B1_D0048.tif" />

The compounds of the invention have a molecular weight less than or equal to about 700 Daltons, and more preferably between about 400 and 600 Daltons. Such beneficial compounds can be readily absorbed into the patient's bloodstream after oral administration. Such oral availability makes the compounds excellent agents for oral administration in the treatment or prevention of IL-1 related diseases.

The ICE inhibitors of the invention can be synthesized using conventional methods. Preferably, these compounds are conveniently synthesized from readily available starting materials.

Compared to the known ICE inhibitors, the compounds of the present invention are most easily prepared. ICE inhibitors described so far often contain four or more chiral centers and multiple peptide bonds. The relative ease with which the compounds of the invention can be synthesized offers great advantages for their commercial production.

It should be understood that the compounds of the invention may exist in a variety of equivalent forms depending on the conditions including solvent selection, pH, and others known to those skilled in the art. All such forms are expressly within the scope of the present invention. In particular, many of the compounds of the invention, especially those containing aldehyde or ketone groups in R3 and carboxylic acid groups, may take the hemi-ketal (or hemi-acetal) or hydrated form as shown below:

(ECH)

R ^ -Ν-Χ, oh =

Η \ I (CHdg-ę-Ro

OH

Rf — N-Xt =

H \ (CHter-c - ** (CJ2) mC \

Ri — N — Xi P

H \ / (Ot) g — C — Rn OH hydrated form hemi-ketal or hemi-acetal

Depending on the choice of the solvent and other conditions known to the person skilled in the art, the compounds of the invention may also exist in the acyloxyketal, acyloxyacetal, ketal or acetal form:

<img file="PL193391B1_D0049.tif" />

(EQ2) oCJinr-C ^

Rf-Ν-Χι w - Rf — N-Χι OR (CFtóg^-ê ~ Rtt H (O-fc) g — Ru

About OR

Acyloxyketal or Ketal or acetal acyloxyacetal

Furthermore, it should be understood that the equivalent forms of the compounds of the invention may include tautomeric forms. All such forms are expressly included within the scope of the present invention.

It should be understood that the compounds of the invention can be modified with appropriate functional groups to increase selective biological properties. Such modifications are known in the art and include methods of increasing biological penetration into a given biological system (e.g. blood, lymphatic system, central nervous system), increasing oral availability, increasing solubility to enable administration by injection, altering metabolism, or altering secretion rate. Furthermore, the compounds of the invention can be converted into prodrugs such that the desired compound is formed in the body of the patient as a result of the action of a metabolic process or other biochemical processes on the prodrug. Examples of such prodrug forms are the ketal, acetal, oxime and hydrazone of compounds which contain ketone or aldehyde groups, especially when these are present in the R3 group of the compounds of the invention.

The compounds of the invention are excellent ligands for ICE. Thus, these compounds are capable of target inhibiting the processes of IL-1 related diseases, such as the conversion of the IL-1b precursor to mature IL-1b, and ultimately inhibiting the activity of this protein in inflammatory diseases, autoimmune and neurodegenerative diseases. For example, compounds of the invention inhibit the conversion of the IL-1b precursor to mature IL-1b by inhibiting ICE. Since ICE is essential in the production of mature IL-1, inhibition of this enzyme effectively blocks the onset of IL-1 related physiological effects and symptoms by preventing the production of mature IL-1. Thus, by inhibiting the activity of the IL-1b precursor, the compounds of the invention are effective as inhibitors of IL-1.

PL 193 391 B1

The compounds of the invention can be used in conventional manner to treat IL-1 mediated diseases. Treatment methods, dosage levels, and requirements can be selected by one skilled in the art from methods and techniques available to them.

Also within the scope of the invention is a pharmaceutical composition which comprises a compound of the invention as an active ingredient and a pharmaceutically acceptable carrier.

Preference is given to a pharmaceutical composition which contains as active ingredient a compound according to the invention in which R 1 is a group of formula (e1) or (e2), especially a compound selected from the group consisting of the following compounds:

<img file="PL193391B1_D0050.tif" />

PL 193 391 B1

The compounds in such compositions may be used alone or together with other compounds of the invention in a manner compatible with the conventional use of ICE inhibitors in pharmaceutical compositions. For example, a compound of the invention can be combined with pharmaceutically acceptable vaccine adjuvants and administered in a prophylactically effective amount to protect the patient from IL-1 related diseases over an extended period of time.

Compounds of the invention may also be co-administered with other ICE inhibitors to increase the efficacy of treatment or prophylaxis against various IL-1 related diseases.

In addition, the compounds of the invention may be used in combination with other conventional anti-inflammatory agents or with matrix metalloprotease inhibitors, lipoxygenase inhibitors, and antagonists of cytokines other than IL-1b.

To prevent or combat the symptoms of IL-1 related diseases such as inflammation, the compounds of the invention can also be administered in combination with immunomodulators (e.g., bropyrimine, anti-human interferon alpha, IL-2, GM-CSF, methionine enkephalin, interferon alpha) , diethyl dithiocarbamate, tumor necrosis factor, naltrexone and rEPO), or with prostaglandins.

When the compounds of the invention are administered in combination therapy with other agents, they may be administered sequentially or simultaneously to the patient.

The pharmaceutical compositions of the invention include any of the compounds of the invention, pharmaceutically acceptable salts thereof, and any pharmaceutically acceptable carrier, excipient or diluent. Pharmaceutically acceptable carriers, excipients and diluents that can be used in the pharmaceutical compositions of the invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins such as human serum albumin, buffering substances such as such as phosphates, glycine, sorbic acid, potassium sorbate, mixtures of partial glycerides of saturated fatty acids of vegetable origin, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinylpyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, polypropylene-blocking polymers , polyethylene glycol and lanolin.

The pharmaceutical compositions of the invention may be administered orally, parenterally, by inhalation inhalation, topically, rectally, nasally, buccally, vaginally and via an implanted reservoir. Oral administration is preferred. The pharmaceutical compositions of the invention may contain any conventional non-toxic pharmaceutically acceptable carriers, adjuvants and diluents. The term parenteral as used herein includes subcutaneous, intradermal, intravenous, intramuscular, intraarticular, intrasynovial, intrasternal, intrathecal, intralesional, intracranial injection and infusion.

The pharmaceutical compositions of the invention may be in the form of a sterile injectable preparation, for example, in the form of a sterile injectable aqueous or oily suspension. The suspension is prepared according to methods known in the art using suitable dispersing or wetting agents (such as, for example, Tween 80) or suspending agents. The sterile injectable preparation may also be in the form of an injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable diluents and solvents, mannitol, water, Ringer's solution, and isotonic sodium chloride solution can be mentioned. Furthermore, it is preferred that sterile, liquid oils are used as a solvent or suspending medium. For this purpose any bland liquid oil may be employed, including synthetic mono- or di-glycerides. Fatty acids, such as oleic acid and its glyceride derivatives are suitable for the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated forms. These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as Ph. Helv. or the like.

The pharmaceutical compositions of the invention may be orally administered in any orally acceptable dosage form, including, but not limited to, capsules, tablets, suspensions, and aqueous solutions. In the case of tablets for oral administration, common carriers can be used, including lactose and corn starch. Usually adds

Lubricants such as magnesium stearate are also included. In capsules for oral administration, suitable diluents include lactose and dry corn starch. When aqueous suspensions are administered orally, the active ingredient is combined with emulsifying and suspending agents. Some sweetening and / or flavoring agents and / or coloring agents can also be added if desired.

The pharmaceutical compositions of the invention may also be administered in the form of rectal suppositories. Such compositions are prepared by mixing a compound of the invention with a suitable non-irritating excipient which is solid at room temperature but liquid at the rectal temperature and thus will melt in the rectum to release the active ingredient. Such excipients include, but are not limited to, cocoa butter, beeswax, and polyethylene glycols.

When the desired treatment involves sites or organs readily accessible by topical application, the compositions of the present invention for topical administration are particularly useful. For topical application to the skin, the pharmaceutical compositions of the invention should be formulated in a suitable ointment containing the active compounds suspended or dissolved in a carrier. Carriers for topical administration of the compounds of the invention include, but are not limited to, mineral oil, liquid petroleum jelly, white petroleum jelly, propylene glycol, polyoxyethylene-polyoxypropylene compound, emulsifying wax, and water. Alternatively, the pharmaceutical compositions may be formulated as suitable lotions or creams containing the active compound suspended or dissolved in a carrier. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl ester waxes, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water. The pharmaceutical compositions of the invention may also be applied topically to the lower intestinal tract in the form of rectal suppositories or enema preparations. Topical transdermal patches are also within the scope of the invention.

The pharmaceutical compositions of the invention may be administered by nasal spray or inhalation. Such compositions are prepared by methods well known in the art and may be formulated in saline solutions using benzyl alcohol or other suitable preservatives, bioavailability enhancing absorption promoters, fluorocarbons, and / or other solubilizing or dispersing agents known in the art.

IL-1 related diseases that can be treated or prevented using the compounds of the invention include, but are not limited to, inflammatory, autoimmune, and neurodegenerative diseases.

Inflammatory diseases that may be treated or prevented include, for example, septic shock, sepsis, and adult respiratory distress syndrome. Autoimmune diseases include, for example, rheumatoid arthritis, systemic lupus erythematosus, scleroderma, chronic thyroiditis, Graves' disease, autoimmune gastritis, insulin dependent diabetes mellitus, autoimmune hemolytic anemia, autoimmune neutropenia, thrombocytopenia, chronic active hepatitis, and myasthenia gravis, multiple sclerosis. Target neurodegenerative diseases include, for example, amyotrophic lateral sclerosis, Alzheimer's disease, Parkinson's disease, and primary lateral sclerosis. The ICE inhibitors of the invention can also be used as wound healing adjuvants. Finally, the ICE inhibitors of the invention can also be used to treat infectious diseases.

While the present invention focuses on the use of the compounds disclosed herein for the treatment and prevention of IL-1 related diseases, the compounds of the invention may also be used as inhibitors of other cysteine ​​proteases.

The compounds of the invention are also useful as commercial reagents that effectively bind to ICE or other cysteine ​​proteases. As commercial reagents, the compounds of the invention and their derivatives may be used to block the proteolysis of a given peptide or may be derivatized and designed to bind to a stable resin as binding substrates for use in affinity chromatography. These and other uses that characterize commercially available cysteine ​​protease inhibitors will be apparent to one skilled in the art.

For a more complete understanding of the present invention, the following examples are provided below. These examples are provided for illustration only and are not intended to limit the scope of the invention in any way.

PL 193 391 B1

P rhek ladi

The following example shows a method of designing a drug that is encompassed by the present invention:

Step i) Two hydrogen bonding residues in ICE are selected, here, the C = O backbone and NH in Arg-34i.

Step 2) The scaffold is selected, here, a pyridone derivative, ensuring that the hydrogen bonding moieties in the scaffold are capable of forming satisfactory hydrogen bonds with the hydrogen bonding moieties selected in step i. Such confirmation is achieved using molecular mechanics techniques to minimize the fragment. scaffolding in the context of the ICE active center:

<img file="PL193391B1_D0051.tif" />

Step 3) The hydrophobic cavity is selected, here S2 as the next target, and the hydrophobic moiety, here benzene. To obtain a hydrophobic overlap, the benzene group within the cavity of S2 is minimized.

<img file="PL193391B1_D0052.tif" />

Step 4) Another hydrophobic cavity is selected, here S4 as the next target, and a hydrophobic moiety, here benzene. The benzene group within the S4 cavity is minimized to obtain a hydrophobic overlap.

<img file="PL193391B1_D0053.tif" />

Step 5) The Si polar cavity is filled with an electronegative residue, here with a carboxylate side chain provided by aspartic acid, in which the C-terminus has been reduced to the aldehyde. Minimized to ensure that the carboxylate side chain is in favorable electrostatic interaction with the polar Si cavity.

<img file="PL193391B1_D0054.tif" />

Step 6) Bind the scaffold to the residues of steps 3, 4 and 5, preferably using the minimum number of bonds consistent with a chemically justified structure. The entire complex molecule in the active center of ICE is reduced.

PL 193 391 B1

<img file="PL193391B1_D0055.tif" />

Step 7) The energy of the molecule is calculated when it has the conformation necessary to bind to ICE. Then the energy is minimized and recalculated - this is the conformational energy of a free compound. The strain energy of binding of a potential inhibitor to ICE is the difference between the conformational energy of the free compound and the conformational energy of the bound compound. The stress energy should be less than about 0.0418 kJ / mol. When the conformational energy of the bond is -0.0067 kJ / mol and the conformational energy of the free compound is -0.0498 kJ / mol, the stress energy is 0.0422 kJ / mol.

Step 8) An inhibitor designed according to the above steps was prepared having a Ki value of 150 nM.

Example 2

Inhibition constants (Ki) and IC50 values ​​were obtained for several compounds of the invention using the methods described below

1. Enzyme test with UV visible substrate

The study was conducted with the succinyl-Tyr-Val-Ala-Asp-p-nitroanilide substrate. The synthesis of analogous substrates was described by LA Reiter (Int. J. Peptide Protein Res. 43, 87-96 (1994). The test mixture contained:

ml buffer (10 mM Tris, 1mM DTT, 0.1% CHAPS @ pH 8.1) ml ICE (50 nM final concentration, 1mOD / min rate) ml DMSO / inhibitor mixture ml 400 mM substrate (80 mM final concentration)

100 ml total volume of the reaction mixture.

The visible ICE assay was performed in a 96 well microtiter plate. Buffer, ICE, and DMSO (when inhibitor is present) were added to the wells in that order. Compounds were incubated at room temperature for 15 minutes, starting when all compounds were placed in all wells. The microtiter plate reader was set to the incubation temperature of 37 ° C. After 15 minutes of incubation, substrate was added directly to the wells and the reaction monitored by chromophore (pNA) release study at 405-603 nm at 37 ° C for 20 minutes. A linear data plot was drawn up and the rate was calculated in mOD / min. DMSO was only used in the inhibitor studies and the buffer was used to bring the volume up to 100 ml in the remaining experiments.

2. Enzymatic studies with a fluorescent substrate

The study was conducted essentially according to Thornberry et al. (Nature 356, 768-774 (1992)) using substrate 17 presented in this article. This substrate is Acetyl-Tyr-Val-Ala-Asp-amino-4-methyl coumarin (AMC).

The following ingredients were mixed:

ml buffer (10 mM Tris, 1 mM DTT, 0.1% CHAPS @pH 8.1) ml CH (2-10 nM final concentration) ml DMSO solution / inhibitor ml 150 mM substrate (30 mM final concentration)

100 ml total volume of the reaction mixture.

The assay was performed in a 96-well microtiter plate. Buffer and ICE were added to the wells. Compounds were allowed to incubate at 37 ° C for 15 minutes while controlling the temperature in the wells. After 15 minutes of incubation, the reaction was started by direct addition of substrate to the well and monitored @ 37 ° C for 30 minutes by AMC fluorophore release using 380 nm excitation and 460 nm emission wavelength. A linear plot of the data was made for each well and the rate was determined in units of fluorescence per second.

PL 193 391 B1

To determine the enzyme inhibition constant (Ki) or the mode of inhibition (competitive, non-competitive and non-competitive), data obtained from enzyme studies with varying inhibitor concentrations were computer fitted to standard enzyme kinetics equations (see

H. Segel, Enzyme Kinetics, Wileynterscience, 1975).

3. Cell research

IL-1b studies using mixed populations of human peripheral blood mononuclear cells (PBMCs) or enriched adherent mononuclear cells.

The processing of pre-IL-1b by ICE can be measured in cell culture using a variety of cell sources. Human PBMCs from healthy donors have produced a mixed population of lymphocyte subtypes and mononuclear cells that produce multiple interleukins and cytokines in response to many types of physiological stimulators. An enriched source of normal monocytes for selective studies of cytokine production by activated cells was obtained from adherent mononuclear cells from PBMC.

Experimental procedure:

A series of initial dilutions of test compounds in DMSO or ethanol were prepared, with sequential dilutions in RPMI-10% FBS medium (containing 2 mM L-glutamine, 10 mM HEPES, 50 U, and 50 mg / ml pen / strep), respectively, to obtain drugs at 4x the final test concentration containing 0.4% DMSO or 0.4% ethanol. Final DMSO concentration is 0.1% for all diluted agents. A concentration titer, which includes the putative Ki value of a test compound as determined by an ICE inhibition assay, is generally used for primary compound screening.

Typically, compounds were tested at 5-6 dilutions and the cellular component of the assay was run in duplicate, with duplicate ELISA determinations for each cell culture supernatant.

PBMC isolation and IL-1 assay

The buffy coat cells were isolated from one pint of human blood (yielding 40-45 ml final plasma volume with cells), diluted to 80 ml with medium and 10 ml of cell suspension was applied to each Leuko-PREP separation tube (Becton Dickinson). After 15 minutes of centrifugation at 1500-1888 xg, the plasma / medium layer was aspirated, then the mononuclear cell layer was collected with a pasteur pipette and transferred to a 15 ml conical centrifuge tube (Corning). Medium was added to bring the volume to 15 ml, cells were gently mixed by inversion and centrifuged at 300x g for 15 minutes. The PBMC pellet was resuspended in a small volume of medium, cells were counted and diluted to 6 x 10 cells / ml.

For the cell assay, 1.0 mL of cell suspension, 0.5 mL of test compound dilution, and 0.5 mL of LPC solution (Sigma # L-3012; 20 ng / ml of solution made up in complete RPMI medium; final LPS concentration 5 ng / ml). Usually, the addition of 0.5 ml of the test compound and LPS is sufficient to mix the contents of the wells. Controls were performed with three mixes per study, with LPS only, solvent-vehicle controls, and / or additional media to bring the final culture volume to 2.0 mL. Cell cultures were incubated for 16-18 hours at 37 ° C in the presence of 5% CO 2.

At the end of the incubation period, cells were harvested and transferred to 15 ml conical centrifuge tubes. After centrifugation for 10 minutes at 200 xg, the supernatants were collected and transferred to

And, 5 Eppendorf tubes. It should be noted that cell pellets can be used for biochemical studies of pre-IL-1bi / or mature IL-1b in cytosolic extracts by western blotting or ELISA with antisera specific for pre-IL-1b.

Isolation of adjacent mononuclear cells

PBMCs were isolated and prepared as described above. Medium (1.0 ml) was added to the wells first, followed by 0.5 ml of the PBMC suspension. After 1 hour of incubation, the plates were gently shaken and non-adherent cells were aspirated from each well. The wells were then washed gently three times with 1.0 ml of medium and finally resuspended in 1.0 ml of medium. The enrichment for adherent cells was 2.5-3.0 x 10⁵ cells per well. The addition of test compounds, LPS, cell incubation conditions and processing of supernatants were performed as described above.

ELISA

The Qantikine kit (R&amp;D Systems) was used to measure mature IL-1b. The test was carried out in accordance with the manufacturer's recommendations. Mature IL-1b levels of approximately 1-3 ng / ml were observed.

PL 193 391 B1 in both PBMC and positive adherent mononuclear cell controls. ELISA studies were performed with 1: 5, 1:10 and 1:20 dilutions of the positive control supernatants to select the optimal dilution for the supernatant in the test panel.

The inhibitory capacity of compounds is represented by the IC50 values, which is the inhibitor concentration at which 50% of the mature IL-1b is detected in the supernatant compared to the positive control.

Using the tests described, the following Ki and IC50 values ​​were determined for compounds A to N. The formulas of compounds A to N are shown in the table below.

<td rowspan="2">Relationship</td><td colspan="3">Ki (mM, in the indicated tests)</td>

<td>UV-visible</td><td>Fluorescence</td><td>Cells</td>

<td></td><td>Ki mM</td><td>Ki mM</td><td>IC50 (mM)</td>

<td>AND</td><td> 5,5</td><td></td><td> 25,0</td>

<td>B</td><td> 8,6</td><td></td><td> 20,0</td>

<td>C.</td><td> 10</td><td></td><td> &gt;30</td>

<td>D</td><td> 4,7</td><td></td><td></td>

<td>E.</td><td> 3,2</td><td></td><td></td>

<td>F.</td><td> 0,15</td><td></td><td> 2 -4</td>

<td>G.</td><td> 4,8</td><td></td><td></td>

<td>H.</td><td> 0,023</td><td> 0,0047</td><td> 6 -11</td>

<td>AND</td><td> 0,0072</td><td> 0,0052</td><td> 2,6</td>

<td>J.</td><td> 0,012</td><td> 0,0039</td><td> 5 -7</td>

<td>K.</td><td> 0,010</td><td> 0,0020</td><td> 2 -11</td>

<td>L.</td><td> 0,014</td><td></td><td></td>

<td>M.</td><td> 0,15</td><td></td><td></td>

<td>N</td><td> 0,95</td><td></td><td></td>

Formulas of compounds A to N:

<img file="PL193391B1_D0056.tif" />

PL 193 391 B1

<img file="PL193391B1_D0057.tif" />

PL 193 391 B1

<img file="PL193391B1_D0058.tif" />

PL 193 391 B1

Example 3

The compounds of Example 2 were synthesized as follows:

H. N- (N-Acetyl-tyrosinyl-valinyl-pipecolyl) -3-amino-4-oxobutanoic acid

Step A. N- (tert-butoxycarbonylpipecolyl) -4-amino-5-benzyloxy-2-oxotetrahydrofuran

The reaction of N-tert-butoxycarbonylpipecolinic acid (460 mg, 2.0 mmol) and N-allyloxycarbonyl-4-amino-5-benzyloxy-2-oxotetrahydrofuran (530 mg, 1.82 mmol) was carried out analogously to that described by Chapman (Bioorg . &amp; Med. Chem. Lett. 1992, 2, 613-618) to obtain 654 mg of the title compound.

¹H NMR (500 MHz, CDCl3) (present as rotamers) d 7.35 (m, 5H), 6.88 (br s, 1H), 4.9-4.45 (m, 4H), 3.95 (br m, 2H), 3.06 (m, 1H), 2.9 (m, 1H), 2.7 (br m, 1H), 2.45 (m, 1H), 2.2 (m, 1H), 1.7-1.5 (m, 3H), 1.45 (two s, 9H).

Step B. N-pipecolyl-4-amino-5-benzyloxy-2-oxotetrahydrofuran

N- (N-tert-butoxycarbonylpipecolyl) -4-amino-5-benzyloxy-2-oxo-tetrahydrofuran (654 mg) was dissolved in 15 mL of 25% trifluoroacetic acid in dichloromethane and stirred at room temperature. The mixture was concentrated to give a gummy residue. This residue was dissolved in dichloromethane and washed with 10% sodium bicarbonate. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated to give 422 mg of the title compound as a beige solid.

¹H NMR (500 MHz, CDCl 3) d 7.38 (m, 5H), 7.15 (d, 1H), 5.55 (d, 1H), 4.95-4.8 (m, 1H), 4 , 78 (m, 1H), 4.65 (d, 1H), 4.45 (m, 1H), 3.2 (m, 0.5H), 3.05 (m, 0.5H), 2. 95 (m, 0.5H), 2.85 (m, 0.5H), 2.65 (m, 1H), 2.55-2.38 (m, 1H), 1.95 (m, 1H) , 1.8 (m, 1H), 1.6 (m, 2H), 1.38 (m, 2H).

Step C. N- (N-acetyl-tyrosinyl-valinyl-pipecolyl) -4-amino-5-benzyloxy-2-oxotetrahydrofuran

N-acetyl-tyrosinyl-valine (464 mg, 1.44 mmol) and N-pipecolyl-4-amino-5-benzyloxy-2-oxotetrahydrofuran (412 mg, 1.3 mmol) were dissolved in 5 mL dimethylformamide and 5 mL dichloromethane and cooled to 0 ° C. To the cooled solution was added 1-hydroxybenzotriazole (HOBT; 210 mg, 1.56 mmol) followed by 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC; 326 mg, 1.7 mmol). After stirring for 18 hours, the mixture was diluted with ethyl acetate and washed with water, 10% sodium hydrogen sulfate solution, 10% sodium bicarbonate solution, and water. The organic layer was concentrated to give a crude solid which was purified by flash chromatography (SiO2) eluting with dichloromethane: isopropanol: pyridine 94: 6: 1 to give 370 mg of the title compound.

¹H NMR (500 MHz, CD3OD) (exists as both diastereomers and rotamers)) d 7.35 (m, 5H), 7.05 (m, 2H), 6.68 (m, 2H), 5, 65-5.25 (m, 1H), 4.9-3.95 (m, 8H), 3.4-2.6 (m, 4H), 2.5-2.1 (m, 1H), 1.98 (s, 1H), 1.9 (s, 1H), 1.85 (s, 1H), 1.8-1.6 (m, 2H), 1.55-1.3 (m, 4H), 0.95-0.85 (m, 6H).

Step D. N- (N-Acetyl-tyrosinyl-valinyl-pipecolyl) -3-amino-4-oxobutanoic acid

To a solution of 100 mg of N- (N-acetyl-tyrosinyl-valinyl-pipecolyl) -4-amino-5-benzyloxy-2-oxotetrahydrofuran in 10 ml of methanol was added 60 mg of Pd (OH) 2 on carbon and the mixture under a balloon under a hydrogen atmosphere . The mixture was filtered through Celite and concentrated to give a white solid. This crude material was dissolved in 2 mL of methanol and triturated with diethyl ether to give mg of the title compound.

₁¹H NMR (500 MHz, CD3OD) (both diastereomers and rotamers)) d 7.1 (m, 2H), 6.7 (m, 2H), 5.2 (brm, 1H), 4.8 -3.6 (m, 6H), 3.2-3.5 (m, 4H), 2.5-2.1 (m, 1H), 1.95 (three s, 3H), 1.9- 1.3 (m, 6H), 1.1-0.7 (m, 6H).

The following compounds were prepared in a manner analogous to that described for compound H:

J. N- [N-acetyl-tyrosinyl-valinyl) - (4-hydroxyprolinyl)] - 3-amino-4-oxobutanoic acid

N-tert-butoxycarbonyl-4-benzyloxyproline was used instead of N-tert-butoxycarbonylpipecolinic acid.

L. N- [2- (N-Acetyl-tyrosinyl-valinyl) - (S) -1,2,3,4-tetrahydroisoquinoline-3-carbonyl] -3-aminooxobutanoic acid

N-tert-butoxycarbonylpipecolinic acid was replaced with (S) -N-tert-butoxycarbonyl-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid.

I. N- (N-acetyl-tyrosinyl-valinyl) - (4-phenoxyprolinyl) -3-amino-4-oxobutanoic acid

Step A. N-tert-butoxycarbonyl-4-phenoxyproline methyl ester

To a cooled (0 ° C) solution of N-tert-butoxy-cis-4-hydroxyproline (2.0 g, 8.15 mmol), phenol (0.77 g, 8.15 mmol) and triphenylphosphine (2.14 g, 8, 15mmol) in 20mL of tetrahydrofuran was added dropwise over 30 minutes to diethyl azodicarboxylate (1.4ml, 9mmol). The reaction mixture was stirred at temperature

After room temperature for 6 hours, it was concentrated to a sticky residue. The crude residue was purified by flash chromatography (SiO2) eluting with a 3: 7 mixture of ethyl acetate and hexane to give 89 g of the title compound.

^andH NMR (500 MHz, CDCl 3) d 7.3 (m, 2H), 6.95 (m, iH), 6.85 (d, 2H), 4.9 (br m, iH), 4.55- 4, i5 (m, 2H), 3.88-3.65 (m, iH), 3.70 (s, 3H), 2.58 (m, iH), 2.22 (m, iH), and . 4 (3 xs, 9H).

Step B. 4-phenoxyproline methyl ester hydrochloride

A cooled solution (ice bath) of N-tert-butoxycarbonyl-4-phenoxyproline methyl ester (0.6 g) in 20 mL of ethyl acetate was bubbled with anhydrous hydrogen chloride until saturation. The mixture was warmed to room temperature and stirred for 3 hours then concentrated to give 480 mg of the title compound.

^andH NMR (500 MHz, CDCl 3) d 7.22 (m, 2H), 6.95 (m, iH), 6.83 (m, 2H), 5, i (br., IH), 4.6 (brm , iH), 4.06 (brm, iH), 3.75 (s, 3H), 3.55 (brm, iH), 2.58 (m, 2H).

Step C. N-acetyl-tyrosinyl-valinyl- (4-phenoxy) proline methyl ester

N-acetyl-tyrosinyl-valine (0.524 g, 1.63 mmol) and 4-phenoxyproline methyl ester (0.381 g, 48 mmol) were dissolved in 4 ml of dimethylformamide and 4 ml of dichloromethane and cooled to 0 ° C. To a chilled solution of added dodenedisopropylethylamine ( 258mL, 1.86mmol), HOBT (0.24g, 1.78mmol), and EDC (0.37g, 1.92mmol) and the reaction mixture was stirred for 18h. The mixture was diluted with 400 mL of ethyl acetate and washed with water, 10% sodium bisulfate, 10% sodium bicarbonate, and water. The organic layer was concentrated to a residue which was purified by flash chromatography (SiO2) eluting with CH2Cl2: I-PrOH: pyridine 94: 6: 1 to give 360 ​​mg of the title compound.

¹H NMR (500 MHz, CDCl3 (present as rotamers)) d 7.3 (m, 2H), 7.05 (m, 1H), 6.95 (d, 2H), 6.9-6.2 ( 4 xd, 4H), 5.05 (br s, 1H), 4.7-3.94 (m, 5H), 2.93 (m, 1H), 2.82 (m, 1H), 2.65 (m, 1H), 2.2 (m, 1H), 2.05 (m, 1H), 1.95 (s, 3H), 1.86 (m, 1H), 0.98 (d, 3H) . 0.88 (d, 3H).

Step D. N-acetyl-tyrosinyl-valinyl- (4-phenoxy) proline

To a solution of N-acetyl-tyrosinyl-valinyl- (4-phenoxy) proline methyl ester (360 mg, 0.685 mmol) in 8 ml of a mixture of tetrahydrofuran and water (1: 1) was added lithium hydroxide (57 mg, 1.37 mmol) and the mixture was stirred at room temperature for 1 hour. The mixture was acidified with 10% hydrochloric acid to give a white solid that was collected to give 175 mg of the title compound.

¹H NMR (500 MHz, DMSO-d6) d9.2 (br s, 1H), 8.05-7.95 (m, 2H), 7.3 (m, 1H), 7.0-6.9 ( m, 4H), 6.65 (d, 2H), 4.42 (m, 1H), 4.35 (m, 1H), 4.05-3.95 (m, 2H), 3.3 (br s, 2H), 2.75 (m, 1H), 2.55-2.38 (m, 2H), 2.2 (m, 1H), 2.0 (m, 1H), 1.7 (s , 3H), 0.95 (d, 3H), 0.85 (d, 3H).

Step E. N- [N-acetyl-tyrosinyl-valinyl- (4-phenoxy) prolinyl-4-amino-5-benzyloxy-2-oxotetrahydrofuran

The title compound was prepared as described for Compound H, step A, by reacting N-acetyl-tyrosinyl-valinyl- (4-phenoxy) proline and N-allyloxycarbonyl-4-amino-5-benzyloxytetrahydrofuran.

¹H NMR (500MHz, CDCl3 (present as a diastereomeric 1: 1 hemiacetal)) d7.8-6.3 (m, 17H), 5.6 (d, 1H), 5.1-4.15 (m, 5H), 4.15-3.75 (m, 2H), 2.95-2.15 (m, 5H), 2.15-1.95 (m, 1H), 1.9-1.85 ( 2xs, 3H), 1.1-0.75 (m, 6H).

Step F. N - [(N-acetyl-tyrosinyl-valinyl) - (4-phenoxy) prolinyl] -3-amino-4-oxobutanoic acid

The title compound was prepared by the hydrogenolysis method described for compound H in step D.

¹1 H NMR (500 MHz, CD₃OD (exists as a 1: 1 hemiacetal diastereomeric mixture)) d7.25 (m, 2H), 7.10-6.85 (m, 5H), 6.65 (d, 2H), 5.1 (brm, 1H), 4.65-4.05 (m, 5H), 4.0-3.40 (m, 2H), 2.95-2.35 (m, 5H), 2.25 (m, 1H) , 2.05 (m, 1H), 1.85 (s, 3H), 1.0 (d, 3H), 0.95 (d, 3H).

K. N - [(N-acetyl-tyrosinyl-valinyl) - (4-benzyloxy) prolinyl] -3-amino-4-oxobutanoic acid

Step A. Semicarbazone of N- (N-allyloxycarbonyl-4-benzyloxyprolinyl-3-amino-4-oxobutanoic acid tert-butyl ester)

The title compound was prepared by reacting N-allyloxycarbonyl-4-benzyloxyproline and 3-amino-4-oxobutanoic acid tert-butyl ester semicarbazone (TL Graybill et al., Abstract of papers, 206th National Meeting of the American Chemical Society, Abstract MEDI-235 Chicago, IL (1993)) under similar peptide coupling conditions as described above (compound H, step C).

¹H NMR (500 MHz, CDCl 3) d 9.05 (br s, 1H), 7.85 (br m, 1H), 7.4-7.2 (m, 5H), 7.15 (br s, 1H) ), 6.55 (br s, 1H), 5.9 (m, 1H), 5.1-4.9 (brm, 2H), 4.65-4.4 (m, 4H), 4.2 ( brm, 1H), 3.75-3.5 (m, 2H), 2.75-2.55 (m, 2H), 2.5 (brm, 1H), 2.25 (brm, 1H), 1 . 4 (s, 9H).

PL 193 391 B1

Step B. N- (acetyl-tyrosinyl-valinyl- (4-benzyloxyprolinyl)) -3-amino-4-oxobutanoic acid tert-butyl ester semicarbazone

The title compounds were prepared by reacting N- (N-allyloxycarbonyl-4-benzyloxyprolinyl) -3-amino-4-oxobutanoic acid tert-butyl ester with N-acetyl-tyrosinyl-valine under the reaction conditions described for compound H, step A.

¹H NMR (500 MHz, CD 3 OD) d 7.35-7.2 (m, 6H), 7.0 (d, 2H), 6.65 (d, 2H), 4.85 (m, 1H), 4 , 6-4.45 (m, 4H), 4.3 (br m, 1H), 4.15 (m, 1H), 3.7 (m, 1H), 2.95 (m, 1H), 2 , 75-2.6 (m, 3H), 2.35 (m, 1H), 2.1 (m, 1H), 1.9 (s, 3H), 1.4 (s, 9H), 0. 95 (d, 3H), 0.90 (s, 3H).

Step C. N- (N-acetyl-tyrosinyl-valinyl- (4-benzyloxyprolinyl)) -3-amino-4-oxobutanoic acid

N- (N-acetyl-tyrosinyl-valinyl- (4-benzyloxyprolinyl)) -3-amino-4-oxobutanoic acid tert-butyl semicarbazone (270 mg) was dissolved in 10 ml of a 25% solution of trifluoroacetic acid in dichloromethane and stirred at temperature room for 3 hours. The mixture was concentrated to a solid residue. This residue was dissolved in 10 ml of a mixture of methanol, acetic acid and 37% formaldehyde (3: 1: 1) and stirred at room temperature for 1 hour. The mixture was concentrated and the resulting residue was purified by flash chromatography (SiO2) eluting with a mixture of dichloromethane / methanol / formic acid (100: 5: 0.5). 37 mg of the title compound were obtained.

¹H NMR (500 MHz, CD3OD (present as a 1: 1 hemiacetal diastereomeric mixture)) d 7.4-7.25 (m, 5H), 7.0 (d, 2H), 6.65 (d, 2H) , 4.65-4.05 (m, 7H), 3.75-3.4 (m, 2H), 3.0-2.3 (m, 5H), 2.2-1.95 (m, 2H), 1.90 (d, 3H), 1.0 (d, 3H), 0.95 (d, 3H).

Example 4

Inhibition constants (Ki) and IC50 values ​​for several compounds according to the invention were obtained in the enzyme test using the uv-visible substrate, and in the cell-based study as described in Example 2. The following Ki and IC50 values ​​for compounds 7a, 7b, 20a-d, 21c-f, 22e, 25, 28, 33a-c, 36a, 39.43, 47a, 47b, 54a-1, 63, 69a, 69b, 84a and 84b. The corresponding letter designations of the compounds are given in parentheses. Compound formulas are shown in examples 2 and 5.

<td colspan="3">Research</td>

<td>Relationship</td><td>UV-visible Ki (mm)</td><td>Cells IC₅₀ (mm)</td>

<td> 1</td><td> 2</td><td> 3</td>

<td>7a</td><td> 35</td><td></td>

<td>7b</td><td> 1,20</td><td></td>

<td>20a (= E)</td><td> 3,20</td><td></td>

<td>20b</td><td> 0,85</td><td> 16,4</td>

<td>20c (= N)</td><td> 0,95</td><td></td>

<td>20d</td><td> 0,10</td><td> 6,2</td>

<td>21c</td><td> 0,64</td><td></td>

<td>21d</td><td> 0,24</td><td> 4,8</td>

<td>21e</td><td> 0,22</td><td> 2,9</td>

<td>21f</td><td> 0,17</td><td> 2,9</td>

<td>22e</td><td> 0,19</td><td></td>

<td> 25</td><td> 6,20</td><td></td>

<td> 28</td><td> 12,00</td><td></td>

<td>33a (= A)</td><td> 5,50</td><td> 25,0</td>

<td>33b (= C)</td><td> 10,00</td><td> &gt;30,0</td>

<td>33c (= B)</td><td> 8,60</td><td> 20,0</td>

<td>36a (= D)</td><td> 4,70</td><td></td>

<td>36b</td><td> 0,80</td><td> 17,0</td>

<td> 39</td><td> 2,500</td><td></td>

To be continued

<td> 1</td><td> 2</td><td> 3</td>

<td> 43</td><td> 20,000</td><td></td>

<td>47a</td><td> 0,019</td><td> 2,1</td>

<td>47b</td><td> 0,027</td><td> 1,8</td>

<td>54a (= F)</td><td> 0,150</td><td> 2,7</td>

<td>54b (= M)</td><td> 0,150</td><td> 9,1</td>

<td>54c</td><td> 1,200</td><td> &gt;19,0</td>

<td>54d</td><td> 1,000</td><td></td>

<td>54e</td><td> 3,500</td><td></td>

<td>54f</td><td> 0,900</td><td></td>

<td>54g (= G)</td><td> 4,800</td><td> &gt;20,0</td>

<td>54h</td><td> 0,970</td><td></td>

<td>54i</td><td> 0,054</td><td> 2,4</td>

<td>54j</td><td> 0,280</td><td></td>

<td>54k</td><td> 0,085</td><td></td>

<td>54l</td><td> 0,215</td><td> 7,0</td>

<td>63 (= O)</td><td> 0,850</td><td> 4,1</td>

<td>69a (= R)</td><td> 0,011</td><td> 0,735</td>

<td>69b (= S)</td><td> 0,050</td><td> 0,745</td>

<td>84a (= V)</td><td> 0,100</td><td> 3,3</td>

<td>84b (= W)</td><td> 0,019</td><td> 0,50</td>

Example 5

Compounds of Example 4 were synthesized as follows:

<img file="PL193391B1_D0059.tif" />

PL 193 391 B1

3-Benzylamino-4-oxo-4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine-6-carboxylic acid methyl ester (3).

A mixture of (4S) -2-amino-1-pyrroline-5-carboxylic acid ethyl ester hydrochloride (1.44 g, 2.38 mmol); obtained by analogy to the preparation of the methyl ester as described by Lee and Lown, J. Org. Chem., 52, 5717-21 (1987)); 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (2.50 g, 2.31 mmol) and sodium methoxide (0.12 g, 2.22 mmol) in ethanol (10 mL) was heated to reflux for 2 hours. The mixture was allowed to cool to room temperature and concentrated in vacuo. The residue was suspended in water and 1N sulfuric acid was added until the pH was 1. The aqueous mixture was extracted with dichloromethane, the organic layer was separated and concentrated in vacuo to give 0.6 g of an orange solid. Chromatography (flash, SiO2, 60% ethyl acetate / hexane, gradually increasing the solvent gradient to 100% ethyl acetate then 10% methanol / dichloromethane) provided 0.5 g of an orange solid. A mixture of this solid and potassium cyanide (0.03 g, 0.5 mmol) in methanol (10 ml) was heated to reflux overnight. After cooling, the reaction mixture was concentrated in vacuo to give a yellow solid. Chromatography (flash, SiO2, 40% ethyl acetate / hexane, gradually increasing the solvent gradient to 100% ethyl acetate) provided 0.22 g (31.6%) of the title compound:

¹H NMR (d6-DMSO) d 2.25 (m, 1H), 2.65 (m, 1H), 3.15 (m, 2H), 3.75 (s, 3H), 5.15 (dd, 1H), 7.5 (t, 2H), 7.6 (t, 1H), 7.95 (d, 2H), 8.6 (s, 1H), 9.5 (s, 1H).

(3S) - [(3-Benzoylamino-4-oxo-4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine-6-carbonyl) -amino] -4-oxobutanoic acid semicarbazone ( 5a and 5b).

A mixture of 3-benzoylamino-4-oxo-4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine-6-carboxylic acid ethyl ester (3.22 g, 0.70 mmol) and hydrate lithium hydroxide (0.032 g, 0.76 mmol) in methanol (5 ml tetrahydrofuran (5 ml) was stirred for 18 hours at room temperature.

The reaction mixture was concentrated to give 3-benzoylamino-4-oxo-4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine-6-carboxylic acid lithium salt (4) as a white solid. This material was used in the next reaction without further purification.

A cooled to 0 ° C mixture of (3S) -amino-4-oxo-butanoic acid tert-butyl ester semicarbazone (0.163 g, 0.71 mmol; Graybill et al., Int. J. Protein Res., 44, p. 173) -83 (1994)) and 3-benzoylamino-4-oxo-4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine-6-carboxylic acid lithium salt (4) in dimethylformamide (5 ml) and dichloromethane (5 mL) was treated with hydroxybenzotriazole (0.104 g, 0.77 mmol) and 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (0.148 g, 0.37 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 18 hours, then poured into water (50 ml) and extracted with ethyl acetate (2 x 50 ml). The combined organic layers were washed with 1M aqueous sodium hydrogen sulfate solution, diluted with aqueous sodium hydrogen carbonate solution (50 ml) and saturated aqueous sodium chloride solution. The layer was concentrated in vacuo to give 0.43 g of a yellow solid. Chromatography (flash, SiO2, ammonium hydroxide / methanol / dichloromethane (1: 1: 99, step gradient, to 1:10:90) gave 0.11 g (30.9%) of the diastereomer with the higher Rf (5a): ¹H NMR (500 MHz, CD 3 OD) d 1.45 (s, 9H), 2.29-2.35 (m, 1H), 2.6-2.7 (m, 2H), 2.8 (dd, 1H), 3.1-3.15 (m, 1H), 3.2-3.3 (m, 1H), 4.9-4.95 (m, 1H), 5.2 (dd, 1H) , 7.25 (d, 1H), 7.5-7.55 (m, 2H), 7.55-7.6 (m, 1H), 7.95 (d, 2H), 8.9 (s , 1H) and 0.11 g (30.9%) of a diastereomer with a lower Rf (5b): ¹H NMR (500 MHz, CD 3 OD) d 1.45 (s, 9H), 2.3-2.4 (m, 1H), 2.6-2.7 (m, 1H), 2.7-2. 8 (m, 2H), 3.1-3.15 (m, 1H), 3.2-3.3 (m, 1H), 4.85-4.95 (m, 1H), 5.15 ( dd, 1H), 7.25 (d, 1H), 7.55 (t, 2H), 7.95 (d, 2H), 8.9 (s, 1H). Diastereomers 5a and 5b were obtained separately.

(3S) - [(3-Benzoylamino-4-oxo-4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine-6-carbonyl) -amino] -4-oxobutanoic acid (7a). (3S) - [(3-Benzoylamino-4-oxo-4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine-6-carbonyl) -amino] -4 acid tert-butyl ester suspension -oxo-butanoic acid (5 [alpha], 0.11 g, 0.22 mmol) in dichloromethane (7.5 mL) and trifluoroacetic acid (2.5 mL) was stirred for 5 hours. The reaction mixture was concentrated in vacuo, the residue was taken up in dichloromethane, concentrated in vacuo, suspended in toluene and concentrated in vacuo to give 0.07 g of the acid semicarbazone (3S) - [(3-benzoylamino-4-oxo-4.6.7, 8-tetrahydro-pyrrolo [1,2-a] pyrimidine-6-carbonyl) -amino] -4-oxo-butane (6a) as a white solid. This material was suspended in a mixture of 37% aqueous formaldehyde, acetic acid and methanol (1: 1: 5) and stirred at room temperature for 18 hours. The reaction mixture was concentrated in vacuo and the residue was suspended in acetonitrile and concentrated in vacuo to yield 0.1 g of a white solid. By chromatography (HPLC, C18 reverse phase, gradient elution 1% to 75% acetonitrile / water (buffer 42

After treatment with 0.1% trifluoroacetic acid)), 0.05 g (60%) of 7a was obtained as a white solid: RT = 7.9 min. (HPLC, C18, reverse phase, 1 to 100% acetonitrile / water (0.1% trifluoroacetic acid buffer); gradient elution 20 min);¹H NMR (CD3OD (present as a mixture of hemiacyloxyacetal anomers, 1: 1) d 2.25-2.4 (m, 1H), 2.45-2.8 (m, 4H), 3.05-3 , 15 (m, 1H), 4.25-4.35 (m, 1H), 4.55-4.6 (m, 1H), 5.1-5.2 (m, 1H), 7.45 -7.65 (m, 3H), 7.9-8.0 (m, 2H), 8.9 (s, 1H).

(3S) - [(3-Benzoylamino-4-oxo-4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine-6-carbonyl) -amino] -4-oxobutanoic acid (7b) was prepared as described for the diastereomer 7a to give 0.03 g (35%) of 7b as a white solid: RT = 8.1 min. (HPLC, C18 reverse phase, 1 to 100% acetonitrile / water (0.1% trifluoroacetic acid buffer); 20 min gradient elution);¹H NMR (d6-DMSO (present as a mixture of hemiacyloxyacetal anomers, 1: 1) d 2.1-2.2 (m, 1H), 2.4 (d, 1H), 2.7-2.8 (m, 1H), 3.0-3.2 (m, 3H), 5.0 (dd, 1H), 5.1-5.2 (m, 1H), 5.5 (s, 1H), 5.75.8 (m, 1H), 7.55 (t, 2H), 7.67 (t, 1H), 7.95 (d, 2H), 8.55 (s, 1H), 9.0 -9.15 (m, 1H), 9.4-9.5 (m, 1H).

<img file="PL193391B1_D0060.tif" />

a R = Η b R = PhCH₂ c R &amp; lt; Ph (CH₂)₂ d R «Ph (CH₂)₃e R = 4MeO-Ph. (CH₂) ₃ f R = 4HO-Ph (CH₂)₃

PL 193 391 B1

The imidazole-2-carboxylic acids were prepared by modifications of the methods described (Yamaka et al., Chem. Pharm. Bull., Pp. 4549-52 (1983)); Suzuki et al., J. Org. Chem., 38, pp. 3571-75 (1973)); and Oliver et al. J. Org. Chem. 38, pp. 1437-38 (1973)).

Imidazole-2-carboxylic acid (13a) was prepared according to Curtis and Brown, J. Org. Chem., 45, pp. 4038-40 (1980).

4-benzylimidazole-2-carboxylic acid (13b) was isolated as an off-white solid; mp temp. 153-155 ° C; IR (KBr) 3026-2624, 1630, 1515, 1498, 1438, 1405;¹H NMR (d 6 -DMSO) d 7.31 (5H, m), 7.14 (1H, s), 3.95 (2H, s).

4- (2-Phenylethyl) imidazole-2-carboxylic acid (13c) was isolated as a pale yellow solid; mp temp. 151-153 ° C; IR (KBr) 3054-2617, 1637, 1497, 1376;¹H NMR (d6-DMSO) d 7.27 (5H, m), 7.11 (1H, s), 2.92 (4H, s).

4- (3-Phenylpropyl) imidazole-2-carboxylic acid (13d) was isolated as a pale yellow solid; mp temp. 148-150 ° C; IR (KBr) 3020-2615, 1636, 1509, 1498, 1383;¹H NMR (d6-DMSO) d 7.35-7.22 (5H, m), 7.01 (1H, s), 2.62 (4H, m), 1.94 (2H, m).

4- [3- (4-methoxyphenyl) propyl] imidazole-2-carboxylic acid (13e), isolated as a white crystalline solid; mp temp. 155-156 ° C (decomposition); IR (KBr) 3300-2300, 1633, 1513, 1376, 1244;¹H NMR (d6-DMSO) d 9.50-7.50 (2H, bs), 7.15 (1H, s), 7.11 (2H, d, J = 8.5), 6.84 (2H , d, J = 8.5), 3.71 (3H, s), 2.60-2.50 (4H, m), 1.86 (2H, m). Analysis Calculated for C14H16N2O3: C, 64.60; H, 6.20; N, 10.76. Found C, 64.45; H, 6.21; N, 10.70.

4- [3- (4-Hydroxyphenyl) propyl] imidazole-2-carboxylic acid (13f). A solution of compound 13e ethyl ester (1.15 g, 4.0 mmol) in dry dichloromethane (50 mL) at 0 ° C was treated with boron tribromide (16 mL, 1.0 M in CH2Cl2, 16.0 mmol). After 15 minutes at 0 ° C, the mixture was warmed to 25 ° C and stirred for 16 hours. The reaction mixture was cooled in an ice bath and quenched by dropwise addition of water (20 mL). The resulting mixture was stirred briefly at 25 ° C and then filtered. The filtrate was thoroughly neutralized by the addition of solid NaHCO3 to afford compound 13f (700 mg, 71%) as a white solid: mp. 186-187 ° C (decomposition) (recrystallized from MeOH); IR (KBr) 3500-2400, 2935, 1640, 1516, 1396, 1232;¹H NMR (d6-DMSO) d 9.83 (3H, bs), 7.16 (1H, s), 6.98 (2H, d, J = 8.2), 6.66 (2H, d, J = 8.2), 2.60-2.40 (4H, m), 1.84 (2H, m). Analysis. Calculated for C13H14N2O3: C, 63.40; H, 5.73; N, 11.38. Found:

C, 62.96; H, 5.70; N, 11.27.

₂ (2R, S, 3S) N²-tert-butoxycarbonyl-N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (14). To a solution of (2R, S, 3S) 3- (N-allyloxycarbonyl) amino-2-benzyloxy-5-oxotetrahydrofuran (Chapman, Biorg. Med. Chem. Lett., 2, pp. 613-618 (1992); (2 , 91 g, 10 mmol)), N-tert-butoxycarbonyl-L-alanine (2.08 g, 11 mmol) and bis (triphenylphosphine) palladium (II) chloride in dichloromethane were added dropwise tri-n-butyltin hydride (4.0 ml, 14.9 mmol) until the color of the solution turned dark orange. Hydroxybenzotriazole (2.70 g, 20 mmol) was added and the mixture was cooled to 0 ° C. 1- (3-Dimethylamino-propyl) -3-ethylcarbodiimide hydrochloride (2.30 g, 12 mmol) was added and the mixture was allowed to slowly warm to room temperature over 4 hours. The mixture was diluted with ethyl acetate (250 ml) and washed with 1N hydrochloric acid (3 x 150 ml), saturated aqueous sodium bicarbonate (3 x 150 ml) and brine (2 x 150 ml), then dried (MgSO4, filtered and concentrated. The crude product was purified by column chromatography (50-70% ethyl acetate / hexane) to afford 3.17 g (84%) of a mixture of diastereomers. Colorless crystals are obtained after recrystallization (ethyl acetate-hexane): mp. 132-145 ° C; IR (KBr) 3357, 3345, 1781, 1688, 1661, 1535, 1517, 1165;¹H NMR (d6-DMSO) d 8.49 (d, J = 6.8), 8.23 ​​(d, J = 7.4), 7.40 (5H, m), 7.01 (1H, m ), 5.68 (d, J = 5.0), 4.75 (m), 4.31 (m), 3.97 (1H, m), 2.82 (m), 3.11 (m) ), 2.82 (m), 2.59 (m), 2.45 (m), 1.40 (9H, s), 1.20 (d, J = 7.2), 1.16 (d , J = 7.2). Analysis. Calculated for C19H26N2O2: C, 60.31; H, 6.92; N, 7.40. Found C, 60.30; H, 6.91; N, 7.38.

(2R, S, 3S) tert-butoxycarbonyl-N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (15) was prepared by the method described for compound 14 to give 1.64 g (81% ) a colorless glassy substance. IR (KBr) 3317, 2978, 1797, 1697, 1670, 1546, 1400, 1366, 1164, 1121;¹H NMR (CDCl3) d 7.68 (1H, brm), 7.35 (5H, m); 5.53 (d, J = 5.2), 5.43 (s), 4.93-4.61 (m), 4.44 (m), 4.25 (brm), 3.39 (2H , brm), 3.10-2.81 (1H, m), 2.44 (1H, m), 2.32 (brm), 1.88 (brm), 1.67 (brm), 1.42 (9H, s).

(2R, S, 3S) N- (N-tert-butoxycarbonyl- (4 (R) -phenoxy-L-prolinyl) -3-amino-2-benzyloxy-5-oxo-tetrahydrofuran (16) was prepared by the method described for the compound 14 to obtain 530 mg (84%) of a colorless amorphous solid: ¹HNMR (CDCl 3) d 7.65 (1H, m), 7.4-7.2 (7H, m), 6.95 (1H, m), 6.85 (1H, m), 5.55 (1H , d), 4.95 (1H, d), 4.8-4.7 (1H, brm), 4.65 (1H, d), 4.55-4.45 (1H, brm), 4. 4-4.3

PL 193 391 B1 (0.5H, brm), 3.95-3.85 (0.5H, brm), 3.75-3.58 (2H, m), 2.95-2.8 (1H, m), 2.7-2.55 (1H, m), 2.54-2.4 (1H, m), 2.35-2.2 (1H, m), 1.4 (9H, s) .

(2R, S, 3S) N²- [4- (3-phenylpropyl) imidazole-2-carbonyl] -N- (tetrahydro-2-benzyloxy-4-oxo-3-furanyl) -L-alaninamide (17d). For a solution of (2R, S, 3S) N²-tert-butoxycarbonyl-N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (14) (1.00 g, 2.64 mmol) in dichloromethane (7 ml) at 0 ° C trifluoroacetic acid (7 ml) was added. The mixture was stirred at 0 ° C for 75 minutes. The mixture was concentrated and the residue was treated with diethyl ether and the ether was removed in vacuo. This procedure was repeated twice to give a pale yellow glassy substance. This material was dissolved in DMF (20 mL). Then, to this solution was added diisopropylethylamine (1.38 ml, 7.92 mmol) followed by 4- (3-phenylpropyl) imidazole-2-carboxylic acid (13d) (0.67 g, 290 mmol), hydrochloride 1- ( 3-dimethylaminopropyl) -3-ethylcarbodiimide (0.56 g, 2.90 mmol) and hydroxybenzotriazole (0.71 g, 5.28 mmol). The mixture was stirred at room temperature for 20 hours then poured into brine. The mixture was extracted with ethyl acetate (3 x 50 ml). The combined organic extracts were washed with saturated aqueous sodium bicarbonate (2 x 100 mL) and brine (2 x 100 mL), dried (MgSO4), filtered and concentrated. The residue was purified by column chromatography (ethyl acetate) to yield 0.99 g (76%) of 17d as a mixture of diastereomers: IR (KBr) 3293, 3064, 2937, 1793, 1650, 1530, 1451, 1446, 1119;¹H NMR (CDCl3) d 7.96 (brm), 7.62 (brd), 7.36-7.10 (10H, m), 6.88 (s), 6.86 (s), 5.53 (d, J = 5.2), 5.48 (s), 4.87-4.52 (4H, m), 3.11-2.38 (2H, m), 2.65 (4H, m ), 1.99 (2H, m), 1.47 (d, J = 6.9), 1.46 (d, J = 7.0).

The following compounds were prepared in a similar manner:

(2R, S, 3S) N²- (imidazole-2-carbonyl) -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (17a) was isolated as a pale yellow solid: IR (KBr) 3289, 3056, 2937, 1793, 1664, 1642, 1528, 1453, 1440, 1124; ¹H NMR (d6-DMSO) d 13.13 (1H, br s), 8.67 (d, J = 7.0), 8.48 (d, J = 7.8), 8.29 (d, J = 6.8), 8.25 (d, J = 7.6), 7.40-7.34 (6H, m), 7.11 (1H, s), 5.69 (d, J = 5 .0), 5.49 (d,

J = 0.8), 4.85-4.31 (4H, m), 3.19-2.42 (2H, m), 1.38 (d, J = 7.4), 1.34 ( d, J = 7.4).

₂ (2R, S, 3S) N²- (4-benzylimidazole-2-carbonyl) -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (17b) was isolated (75%) as a pale yellow glassy substance: IR (KBr) 3294, 3031, 2937, 1792, 1650, 1530, 1453, 1444, 1119; ¹H NMR (CDCl3) d 7.99 (brm), 7.75 (brd), 7.36-7.11 (10H, m), 6.81 (1H, s), 5.51, 5.45 ( d, s, J = 5.3), 4.85-4.47 (4H, m), 3.95 (2H, s), 3.04-2.72 (1H, m), 2.48- 2.35 (1H, m), 1.44 (d, J = 6.9), 1.43 (d, J = 7.1).

(2R, S, 3S) N²- [4- (2-phenylethyl) imidazole-2-carbonyl] -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (17c) was isolated (79%) as a pale yellow glassy substance : IR (KBr) 3292, 3029, 2936, 1793, 1650, 1530, 1453, 1444, 1119; ¹H NMR (CDCl3) d 8.06 (brm), 7.70 (brs), 7.39-7.15 (10H, m), 6.82 (s), 6.81 (s), 5.52 (d, J = 5.2), 5.48 (s), 4.87-4.53 (4H, m), 2.95 (4H, m), 3.14-2.37 (2H, m ), 1.48 (d, J = 6.5), 1.45 (d, J = 6.7).

(2R, S, 3S) 1- [4- (2-phenylethyl) imidazole-2-carbonyl] -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (18c) was isolated ( 79%) as a pale yellow glassy substance: IR (KBr) 3422, 2959, 1795, 1685, 1611, 1497, 1116; ¹H NMR (d6-DMSO) d12.78-12.59 (1H, m), 8.61-8.34 (1H, m), 7.39-7.22 (10H, m), 6.99- 6.61 (1H, m), 5.71-5.26 (1H, m), 4.85-4.02 (4H, m), 3.63 (1H, m), 3.18-1. 74 (11H, m).

(2R, S, 3S) 1- [4- (3-phenylpropyl) imidazole-2-carbonyl] -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (18d) was isolated ( 87%) as a colorless glassy substance: IR (CH2Cl2) 3422, 3214, 2945, 1794, 1685, 1604, 1496, 1117; ¹H NMR (d6-DMSO) d 12.71 (1H, brm), 8.61-8.34 (1H, m), 7.45-7.18 (10H, m), 7.05-6.64 (1H, m), 5.70-5.28 (1H, m), 4.85-4.02 (4H, m), 3.62 (1H, m), 3.18-1.71 (13H , m).

(2R, S, 3S) 1- {4- [3- (4-methoxyphenyl) propyl] imidazole-2-carbonyl} -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (18e) was isolated (72%) as a white glassy solid: mp. 62-65 ° C; (IR) (KBr) 3213, 2937, 1793, 1680, 1606, 1512, 1245;¹H NMR (d6-DMSO) d 12.71, 12.67, 12.58 (1H, 3 x bs), 8.60-8.30 (1H, m), 7.40-7.20 (5H, m), 7.15-6.55 (5H, m), 5.66-5.20 (1H, m), 4.81-4.59 (2H, m), 4.55-4.05 ( 2H, m), 3.71 (3H, s), 3.65-3.45 (1H, m), 3.15-1.50 (13H, m). FABSMS m / e 547 (M⁺, 100%), 439, 412, 340, 312, 243, 177, 154.

(2R, S, 3S) 1- {4- [3- (4-hydroxyphenyl) propyl] imidazole-2-carbonyl} -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (18f) was isolated (70%) as a light yellow glassy solid: mp. 86-90 ° C; IR (KBr) 3298, 1790, 1669, 1606, 1515, 1242;¹H NMR (d6-DMSO) d 12.66, 12.56 (1H, 2xbs), 9.14 (1H, s), 8.57-8.30 (1H, m), 7.36-7. 30 (5H, m), 7.02-6.83 (3H, m),

PL 193 391 B1

6.70-6.57 (2H, m), 5.65-5.28 (1H, m), 4.80-4.49 (2H, m), 4.50-4.05 (2H, m ), 3.65-3.45 (1H, m), 3.15-1.55 (13H, m). FABMS m / e 533 (M⁺, 100%) 425, 298, 229, 176, 154.

1- {5- [3- (4-methoxyphenyl) propyl] -1H-imidazole-2-carbonyl} -4 (R) -phenoxypyrrolidine-2 (S) -carbonyl- (tetrahydro-2 (R, S) -benzyloxy -5-Oxofuran-3 (S) -yl) amide (19e) was isolated (77%) as a clear colorless amorphous solid. ¹H NMR (CDCl3) d 9.95-9.75 (1H, m), 7.95 (1H, br s), 7.40-7.2 (7H, m), 7.2-6.78 (7H , m), 5.65-5.6 (IH, m), 5.55-5.45 (IH, m), 5.3-5.2 (IH, m), 5.15-5.0 (1H, m), 4.95-4.75 (1H, m), 4.7-4.6 (1H, m), 4.5-4.4 (1H, m), 4.35-4 , 25 (1H, m), 3.8 (3H, s), 3.05-1.75 (10H, m).

(3S) 3- {N- [4- (3-Phenylpropyl) imidazole-2-carbonyl] -L-alaninyl} amino-4-oxo-butanoic acid (20d). A mixture of (2R, S, 3S) N²- [4- (3-phenylpropyl) imidazole-2-carbonyl] -N- (tetrahydro-2-benzyloxy-4-oxo-3-furanyl) -L-alaninamide (0.93 g, 1.90 mmol) and 10 % palladium on activated carbon (0.93 g) in methanol (100 ml) was stirred under a hydrogen atmosphere for 5 hours. The resulting mixture was filtered and concentrated to give a colorless glass. After recrystallization from a mixture of methanol and diethyl ether, 401 mg (53%) of 20d were obtained as a colorless solid; mp temp. 94-96 ° C; [a] D²⁷ +1 6.4 ° (c 0.5, MeOH); IR (KBr) 3300, 3287, 1786, 1732, 1659, 1651, 1532, 1451;¹H NMR (CD3OD) d 7.19 (5H, m), 6.91 (1H, s), 4.60-4.46 (2H, m), 4.27 (1H, m), 2.63 (4H , m)

2.75- 2.40 (2H, m), 1.96 (2H, m), 1.44 (3H, d, J = 7.0).

The following compounds were prepared in a similar manner:

(3S) 3- [N- (Imidazole-2-carbonyl) -L-alaninyl] amino-4-oxobutanoic acid (20a, E) was isolated (83%) as a colorless solid: mp. 115 ° C; [a] D²⁵+ 4.4 ° (c 0.5, MeOH); IR (KBr) 3303, 1782, 1658, 1650, 1563, 1521, 1454;¹H NMR (CD3OD) d 7.18 (2H, s), 4.55 (2H, m), 4.27 (1H, m), 2.56 (2H, m), 1.45 (d, J = 7 , 1), 1.44 (d, J = 7.0).

(3S) 3- [N- (4-benzylimidazole-2-carbonyl) -L-alaninyl] amino-4-oxobutanoic acid (20b) was isolated (56%) as a colorless solid: m.p. 113-115 ° C; [a] D²⁹ + 18.2 ° (c 0.5, MeOH). IR (KBr) 3301, 3288, 1783, 1727, 1650, 1521, 1452;¹H NMR (CD 3 OD) d 7.25 (5H, m), 6.90 (1H, s), 4.59-4.45 (2H, m), 4.26 (1H, m), 3.95 (2H , s), 2.74-2.39 (2H, m), 1.42 (3H, d, J = 7.0). Analysis. Calculated for C18H20N4O5: C, 56.69; H, 5.55; N, 14.69. Found C, 57.06; H, 5.54; N, 14.41.

(3S) 3- {N- [4- (2-Phenylethyl) imidazole-2-carbonyl] -L-alaninyl} amino-4-oxobutanoic acid (20c; N) was isolated (53%) as a colorless solid: temp maturity 102-104 ° C; [a] D²⁷+ 13.7 ° (c 0.5, MeOH); IR (KBr) 3299, 3289, 1785, 1732, 1531, 1452;¹H NMR (CD3OD) d 7.20 (5H, m), 6.82 (1H, s), 4.60-4.46 (2H, m), 4.29 (1H, m), 2.92 ( 4H, s), 2.76-2.41 (2H, m), 1.44 (3H, 2xd, J = 7.1). Analysis. Calculated for C19H22N4O5: C, 56.43; H, 5.98; N, 13.85. Found C, 56.65; H, 5.84; N, 13.91.

(3S) 3- {N- [4- (2-Phenylethyl) imidazole-2-carbonyl] -L-prolinyl} amino-4-oxobutanoic acid (21 c) was isolated (85%) as a glassy colorless substance; mp temp. 101-103 ° (methanol-diethyl ether); [a] D²⁷ -63.8 ° (c 0.25, MeOH); IR (KBr) 3275, 1784, 1728, 1664, 1606, 1498, 1429;¹H NMR (CD3OD) d 7.24 (5H, m), 6.83 (s), 6.79 (s), 4.58-4.14 (3H, m), 3.69 (1H, m) . 2.93 (4H, brs),

2.75- 1.99 (6H, m). Analysis. Calculated for C21H24N4O5 H2O: C, 58.60; H, 6.90; N, 13.02. Found C, 58.34; H, 5.96; N, 12.67.

(3S) 3- {N- [4- (3-Phenylpropyl) imidazole-2-carbonyl] -L-prolinyl} amino-4-oxobutanoic acid (21d) was isolated (81%) as a glassy colorless substance; mp temp. 91-94 ° C; (methanol-diethyl ether); [a] D²⁵ -68 ° (c 0.25, MeOH); IR (KBr) 3277, 2939, 1784, 1727, 1662, 1606, 1498, 1429;¹H NMR (CD3OD) d 7.29-7.16 (5H, m), 6.92 (s), 6.86 (s), 4.58-4.16 (3H, m), 3.71 (1H , m), 2.75-1.92 (13H, m). Analysis. Calculated for C22H26N4O5 H2O: C, 59.45; H, 6.35; N, 12.60. Found C, 59.75; H, 6.21; N, 12.41.

(3S) 3- {N- [4- [3- (4-Methoxyphenyl) propyl] imidazole-2-carbonyl] -L-prolinyl} amino-4-oxobutanoic acid (21e) was isolated (65%) as a glassy white solid substance; mp temp. 101-105 ° C; [a] D²³ -60.5 (c 0.05, MeOH); IR (KBr) 3231, 1784, 1726, 1611, 1512, 1245;¹H NMR (CD3OD) d 7.09 (2H, d, J = 8.6), 6.85 (1H, 2xs), 6.81 (2H, d, J = 8.6), 5.45-5 , 30 (1H, m), 4.64-4.46 (1H, m), 4.28-4.10 (2H, m), 3.75 (3H, s), 3.74-3.66 (1H, m), 2.67-1.84 (13H, m). Analysis. Calculated for C23H28N4O6 H2O: C, 58.22; H, 6.37; N, 11.81. Found C, 58.39; H, 6.34; N, 11.45; FABMS m / e 457 (M⁺), 405, 312, 243, 215, 176, 154 (100%).

(3S) 3- {N- [4- [3- (4-Hydroxyphenyl) propyl] imidazole-2-carbonyl] -L-prolinyl} amino-4-oxobutanoic acid (21f) was isolated (43%) as a glassy white solid substance; mp temp. 114-118 ° C; [a] D²⁵ -55.7 (c 0.05, MeOH); IR (KBr) 3288, 2935, 1780, 1715, 1662, 1610, 1515, 1441;¹H NMR (CD3OD) d6.99 (2H, d, J = 8.5), 6.91, 6.85 (1H, 2xs), 6.68 (2H, d, J = 8.5), 5 , 45-5.30 (1H, m), 4.60-4.47 (1H, m), 4.30-4.10 (2H, m), 3.80-3.55 (1H, m) , 2.70-1.80 (13H, m). Analysis. Calculated for

PL 193 391 B1

C22H26N4O6 H2O: C, 57.38; H, 6.13; N, 12.17. Found C, 57.68; H, 6.25; N, 11.66. FABMS m / e 443 (M⁺), 298, 229, 154 (100%).

3 (S) - [(1- {5- [3- (4-methoxyphenyl) propyl] -1H-imidazole-2-carbonyl} -4 (R) -phenoxypyrrolidine-2 (S) -carbonyl) amino] - acid 4-Oxobutane (22e) was isolated (43%) as a beige solid: ¹H NMR (CD3OD) d 7.35-7.2 (3H, m), 7.15-7.0 (2H, m), 6.98-6.85 (3H, m), 6.83-6 . 77 (2H, d), 5.4-5.1 (1H, m), 4.65-4.5 (1H, m), 4.35-4.2 (2H, m), 4.15 -3.90 (1H, m), 3.78 (3H, s), 3.62-3.48 (1H, m), 2.78-2.25 (8H, m), 2.02-1 . 9 (2H, m).

<img file="PL193391B1_D0061.tif" />

{Phenethyl- [5- (3-propyl) -1H-imidazole-2-carbonyl] amino} -acetic acid tert-butyl ester (23). A solution chilled to 0 ° C of 4- (3-phenylpropyl) -imidazole-2-carboxylic acid (13d) (150 mg, 0.65 mmol) and N- (2-phenethyl) glycine tert-butyl ester (140 mg, 0 , 59 mmol) in 5 mL anhydrous dimethylformamide was treated with diisopropylethylamine (154 mL, 0.89 mmol), hydroxybenzotriazole (160 mg, 1.18 mmol), and 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (136 mg, 0.18 mmol). 71 mmol). After stirring for 36 hours, the reaction mixture was poured into a saturated aqueous sodium chloride solution and extracted with ethyl acetate (3 x 50 ml). The combined organic extracts were washed twice with saturated aqueous sodium bicarbonate (2x) and saturated aqueous sodium chloride (1x), dried (Na2SO4), filtered and concentrated in vacuo to yield a brown oil. Chromatography (flash, SiO2, 30% ethyl acetate / hexane) provided 160 mg (61%) of compound 23 as a white solid.¹H NMR (CDCl3) d 7.38-7.14 (10H, m), 6.85-6.8

PL 193 391 B1 (1H, m), 4.84-4.76 (1H, d), 4.5-4.42 (1H, m), 4.07-4.0 (1H, d), 3 , 78-3.72 (1H, m), 3.12-2.94 (2H, 2xm),

2.75-2.55 (4H, m), 2.1-1.95 (2H, m), 1.5-1.45 (9H, 3xs).

(3S) - (2-Phenethyl- [5- (3-phenyl-propyl) -1H-imidazole-2-carbonyl] amino} acetylamino) -4-oxobutanoic acid tert-butyl ester semicarbazone (24). Ester 23 (160 mg, 0.375 mmol) was treated with a 25% solution of trifluoroacetic acid and dichloromethane (7 ml) for 4 hours. The mixture was concentrated in vacuo to give 180 mg of the acid. This acid (180 mg, 0.357 mmol) was coupled with (3S) -3-amino-4-oxobutanoic acid tert-butyl ester semicarbazone (161 mg, 0.357 mmol) as described for the preparation of compounds 5a and 5b to give 86 mg. (33%) compound 24 (one diastereomer) as a white solid. ¹HNMR (CDCl3) d 10.08-9.78 (1H, 2d), 9.25-9.15 (1H, m), 8.35-8.10 (1H, 2m), 7.9-7, 85 (1H, 2s), 7.40-7.05 (10H, m), 6.9-6.75 (1H, m), 6.3-5.8 (1H, br s), 5.2 -4.65 (2H, m), 4.35-3.5 (3H, m), 3.25-3.0 (2H, m), 2.9-2.45 (6H, m), 2 , 05-1.8 (2H, m), 1.4 (9H, s).

(3S) - (2- {Phenethyl- [5- (3-phenylpropyl) -1H-imidazole-2-carbonyl] -amino} acetylamino) -4-oxobutanoic acid trifluoroacetate (25) was prepared as described for 7a to afford 32 mg (82%) of the compound as a white solid: ¹H NMR (CD3OD) d 7.05-7.35 (m, 11H), 4.65 (m, 1H), 4.4 (m, 1H), 4.3 (s, 2H), 3.6- 4.0 (m, 2H), 2.5-2.95 (m, 8H), 2.05 (m, 2H).

7- [5- (3-Phenyl-propyl) -1H-imidazole-2-carbonyl] -1,4-dithia-7-azaspiro [4.4] nonane-8 (S) -carboxylic acid methyl ester (26). 4- (3-Phenylpropyl) imidazole-2-carboxylic acid (13d) was coupled with 1,4-dithia-7-azaspiro [4.4] nonane-8 (S) -carboxylic acid methyl ester hydrobromide (Smith et al. J. Med Chem., 31, pp. 875-85 (1988)) as described for compound 23 to give 140 mg (65%) of the title compound as a yellow gum:¹H NMR (CDCl3) d 7.34-7.15 (5H, m), 6.98-6.8 (1H, 3s), 5.7-5.65 (0.5H, m), 5.2 -5.1 (1H, m), 4.82-4.75 (0.5H, m), 4.4-4.35 (1H, m), 4.05 (1H, d), 3.75 -3.7 (3H, 2s), 3.4-3.3 (4H, m), 2.95-2.45 (8H, m), 2.05-1.95 (2H, m).

(3S) - ({7- [5- (3-Phenyl-propyl) -1H-imidazole-2-carbonyl] -1,4-dithia-7-azaspiro [4.4] nonane-8 (S) acid tert-butyl semicarbazone -carbonyl} -amino) -4-oxobutanoic (27).

According to the procedure described for compound 4, ester 26 was converted to its acid, which was then coupled with (3S) -3-amino-4-oxobutanoic acid tert-butyl ester semicarbazone as described for compound 24 to give 70 mg (33% ) brown solid: ¹H NMR (CD3OD) d 7.28-7.10 (5H, m), 6.90 (1H, br s), 4.94 (1H, m), 3.96-3.86 (2H, q) , 3.35-3.25 (4H, d), 3.0 (2H, s), 2.73-2.59 (6H, m), 2.0-1.92 (2H, m), 1 . 44 (9H, s).

(3S) - ({7- [5- (3-Phenylpropyl) -1H-imidazole-2-carbonyl] -1,4-dithia-7-azaspiro [4.4] nonane-8 (S) -carbonyl} -amino acid ) -4-oxobutane (28) was prepared as described for 7a to give 17 mg (26%) of a light brown solid. ¹H NMR (CD3OD) d 7.4 (s, 1H), 7.1-7.25 (m, 5H), 4.9 (m, 1H), 4.6 (m, 1H), 4.3 (m , 1H), 3.95 (s, 2H), 3.25-3.4 (m, 4H), 3.0 (d, 2H), 2.6-2.8 (m, 5H), 2. 45 (m, 1H), 2.05 (m, 2H).

<img file="PL193391B1_D0062.tif" />

and R = Ph (CH₂)₂ b R = 4CF₃-Ph (CH₂)₂ c R = PhCH₂

PL 193 391 B1

4,5-dihydroimidazole-4-carboxylic acid esters (29) were prepared by modifying the method described by Jones et al., Tetrahedron Lett., 29, pp. 3853-56 (1988).

Methyl (4R, S) 2- (2-phenylethyl) -4,5-dihydroimidazole-4-carboxylate (29a). Dry hydrogen chloride was bubbled through a solution of hydrocinnamonitrile (3.28 mL, 25 mmol) in methanol (125 mL) at 0 ° C for 45 minutes. The solvents were removed to give the imidate which was dissolved in methanol (125 ml) along with methyl 2,3-diaminopropionate (25 mmol) (Jones et al., Supra). The mixture was kept at room temperature for 2.5 hours and then concentrated to a yellow oil. The crude product was purified by column chromatography (10-20% methanol / dichloromethane) to give 3.52 g (61%) of a colorless glassy substance: ¹H NMR (CDCl3) d 7.30-7.15 (5H, m), 4.63 (1H, t, J = 9.7), 3.96 (2H, d, J = 9.7), 3 . 72 (3H, s), 3.10 (4H, m), ¹³C NMR (CDCl3) d171.3, 138.3, 128.4, 128.2, 126.6, 57.3, 53.0, 47.7, 31.7, 27.9.

Methyl (4R, S) 2- [2- (4-trifluoromethylphenyl) ethyl] -4,5-dihydroimidazole-4-carboxylate (29b) was prepared as described for 29a to give 6.80 g (78%) of a colorless solid: mp temp. 136-141 ° C;¹H NMR (CDCl3) d 7.45 (4H, s), 4.71 (1H, dd, J = 8.6, 10.8), 4.02 (2H, m), 3.73 (3H, s) . 3.19 (4H, m).

The imidazole-4-carboxylic acid esters were prepared by modifying the method described by Martin et al., J. Org. Chem., 33, pp. 3758-61 (1968).

Methyl 2- (2-phenylethyl) imidazole-4-carboxylate (30a). A mixture of methyl (4R, S) 2- (2-phenylethyl) -4,5-dihydroimidazole-4-carboxylate (29a) (3.40 g, 14.64 mmol), chloroform (75 ml) and manganese (IV) oxide (13.0 g, 150 mmol) was heated to reflux for 21 hours then filtered hot. The solids were washed with chloroform and methanol. The combined filtrate was concentrated to give a yellow-brown solid which was purified by column chromatography (2-5% methanol / dichloromethane) to afford 1.46 g (43%) of a pale yellow solid: mp. 151-155 ° C; IR (KBr) 3028, 2946, 1720, 1533, 1433, 1348, 1195, 1166;¹H NMR (CDCl3) d 7.62 (1H, s), 7.26-7.02 (5H, m), 3.82 (3H, s), 3.03 (4H, brs), ¹³C NMR (CDCl3) d 162.9, 150.2, 140.3, 128.5, 128.2, 126.3, 51.5, 34.5, 30.4. Analysis. Calculated for C13H14N2O2: C, 67.81; H, 6.13; N, 12.16. Found C, 67.70; H, 6.15; N, 12.16.

Methyl 2- [2- (4-trifluoromethylphenyl) ethyl] imidazole-4-carboxylate (30b) was prepared by the method described for 30a. After recrystallization from ethyl acetate, 1.89 g (33%) of cream-colored crystals were obtained: mp. 225-26 ° C; IR (KBr) 3239, 2951, 1715, 1532, 1331, 1158, 1105, 1068;¹H NMR (CDCl3) d 7.61 (1H, s), 7.54 (2H, d, J = 8.1), 7.26 (2H, d, J = 8.1), 3.89 (3H , s). 3.10 (4H, m). Analysis. Calculated for C14H13F3N2O2: C, 56.38, H, 4.39; N, 9.39; F, 19.11. Found C, 56.23; H, 4.44; N, 9.33; F, 19.08.

2- (2-Phenylethyl) imidazole-4-carboxylic acid (31a). A mixture of methyl 2- (2-phenylethyl) imidazole-4-carboxylate (31a) (1.38 g, 6 mmol), methanol (30 ml) and 1M aqueous sodium hydroxide solution (30 ml) was heated to reflux for 16 hours. The methanol was removed under reduced pressure and the resulting aqueous solution was neutralized with 4M hydrochloric acid, resulting in a yellow solid precipitating. The material was collected, washed with water, and dried. 1.18 g (91%) of a pale yellow solid were obtained: mp. 117-120 ° C; IR (KBr) 3375, 3131, 2616, 2472, 1638, 1592, 1551, 1421, 1388, 1360;¹H NMR (d6-DMSO) d 7.59 (1H, s), 7.26 (5H, m), 2.99 (4H, m). Analysis. Calculated for C12H12N2O2 0.25H2O: C, 65.29; H, 5.71; N, 12.69. Found C, 65.00; H, 5.64; N, 12.58.

2- [2- (4-Trifluoromethylphenyl) ethyl] imidazole-4-carboxylic acid (31b) was prepared as described for 31a to give 1.09 g (76%) of a pale yellow solid: mp. 126-130 ° C; IR (KBr) 3339, 2640-2467, 1638, 1589, 1545, 1383, 1323;¹H NMR (d6-DMSO) d7.69 (2H, d, J = 8.0), 7.59 (1H, s), 7.47 (2H, d, J = 8.0), 3.06 ( 4H, m).

(2R, S, 3S) N²- [2- (2-phenylethyl) imidazole-4-carbonyl] -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (32a). To a 0 ° C solution of (2R, S, 3S) N²-tert-butoxycarbonyl-N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (14) (1.59 g, 4.20 mmol; Chapman, Biorg. Med. Chem. Lett. , 2, pp. 613-18 (1992)) in dichloromethane (15 ml) was added trifluoroacetic acid (15 ml). The mixture was stirred at &lt;RTI ID = 0.0&gt; C &lt;/RTI&gt; for 1 hour and then concentrated. The residue was treated with ether and then the ether was removed in vacuo. This procedure was repeated twice to give a pale yellow glassy substance. This material was dissolved in DMF (20 mL), then diisopropylethylamine (2.19 mL, 12.6 mmol), 2- (2-phenylethyl) imidazole-4-carboxylic acid (31a) (1.0 g, 4.62 mmol), 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (0.89 g, 4.62 mmol) and hydroxybenzotriazole (1.14 g, 8.40 mmol). The reaction mixture was stirred at room temperature for 20 hours then poured into brine. The mixture was extracted with ethyl acetate (3 x 50 ml). The combined organic extracts were washed with saturated aqueous sodium bicarbonate then brine, dried (MgSO4) and concentrated. The residue was purified by column chromatography (2-10% isopropanol in dichloromethane then 0-6% isopropanol in ethyl acetate) to give 1.10 g (55%) of compound 32a as a mixture of diastereomers: IR (KBr) 3278, 3065, 1790 , 1641, 1577, 1545, 1499, 1454, 1120;¹H NMR (CDCl3) d 10.26 (1H, s), 8.14 (1H, s), 7.66 (d, J = 7.0), 7.56 (d, J = 7.0), 7.43 (1H, s), 7.31-7.11 (10H, m), 5.49 (d, J = 5.6), 5.48 (s), 4.83-4.41 ( 4H, m), 3.04-2.41 (2H, m), 2.99 (4H, s), 1.45 (d, J = 7.0), 1.44 (d, J₂ = 7,0).

(2R, S, 3S) N²- {2- [2- (4-trifluoromethylphenyl) ethyl] imidazole-4-carbonyl} -4- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (32b) was prepared as described for 32a to obtain 1.08 g (62%) of a pale yellow glassy substance: IR (KBr) 3376, 3284, 3070, 2938, 1791, 1642, 1578, 1546, 1327, 1165, 1122, 1068; ¹H NMR (CDCl3) d 7.95 (0.5H, m), 7.55-7.25 (11.5H, m), 5.53 (s), 5.49 (d, J = 5.3 ), 4.88-4.48 (4H, m), 3.11-2.96 (4H, m), 2.91 (1H, m), 2.51 (1H, m), 1.47 ( 3H, d, J = 7.1).

(2R, S, 3S) N²- (2-benzylimidazole-4-carbonyl) -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (32c) was prepared as described for compound 32a from 2-benzylimidazole-4-carboxylic acid (Ger. Offen. DE 3427136) to obtain 1.13 g (83%) of a yellow glassy substance: IR (CH2Cl2) 3433, 3062, 2990, 1803, 1693, 1584, 1504, 1429, 1285, 1258; ¹H NMR (CDCl3) d9.50 (s), 9.37 (s), 7.86 (0.5H, d, J = 6.1), 7.56-7.21 (10.5H, m) , 7.48 (1H, s), 5.51 (d, J = 5.2), 5.48 (s), 4.87-4.41 (4H, m), 4.08 (s), 4.07 (s), 3.03-2.39 (2H, m), 1.46 (3H, d, J = 7.0).

(3S) 3- {N- [2- (2-Phenylethyl) imidazole-4-carbonyl] -L-alaninyl} amino-4-oxobutanoic acid (33aA). A mixture of (2R, S, 3S) N²- [2- (2-phenylethyl) imidazole-4-carbonyl] -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (32a) (1.0 g, 2.10 mmol ) and 10% palladium on activated carbon (1.0 g) in methanol (50 ml) were stirred under a hydrogen atmosphere for 4.5 hours. The resulting mixture was filtered and concentrated to give a colorless glass. After recrystallization from a mixture of methanol and diethyl ether, 510 mg (63%) of a colorless solid are obtained: mp. 127 ° C; IR (KBr) 3360, 3279, 2981, 1781, 1732, 1646, 1577, 1547;¹H NMR (CD3OD) d7.54 (1H, s), 7.29-7.12 (5H, m), 4.60-4.47 (2H, m), 4.28 (1H, m), 3 . 01 (4H, s), 2.76-2.39 (2H, m), 1.43 (3H, 2xd, J = 7.0, J = 7.0), ¹³C NMR (CD3OD) d 176.2, 176.0, 174.7, 174.6, 164.4, 164.3, 150.5, 141.9, 134.8, 129.3, 127.3, 122.3, 98.8, 52.3, 52.0, 50.3, 35.6, 31.2, 18.8, 18.7. Analysis. Calculated for C19H22N4O5 H2O: C, 56.43, H, 5.98; N, 13.85; Found C, 56.78, H, 5.70; N, 13.77.

(3S) 3- {N- [2- (2- [4-Trifluoromethylphenyl] ethyl) imidazole-4-carbonyl] -L-alaninyl} -amino-4-oxobutanoic acid (33b; C) was prepared as described for 33a 612 mg (73%) of a colorless solid were obtained: mp. 120-124 ° C; [a] D²³ + 14.3 ° (c 0.5, MeOH); IR (KBr) 3287, 2985, 2937, 1782, 1732, 1646, 1579, 1547, 1327;¹H NMR (CD3OD) d7.56 (2H, d, J = 8.0), 7.54 (1H, s), 7.36 (2H, d, J = 8.0), 4.60-4, 48 (2H, m), 4.28 (1H, m), 3.08 (4H, m), 2.75-2.41 (2H, m), 1.43 (3H, d, J = 7, 0). Analysis. Calculated for C20H21F3N4O5 0.5H2O: C, 51.84; H, 4.78; N, 12.09; F, 12.30. Found C, 51.83; H, 4.72; N, 12.14; F, 12.36.

(3S) 3- [N- (2-benzylimidazole-4-carbonyl) -L-alaninyl] amino-4-oxobutanoic acid (33c, B) was prepared as described for compound 33a to give 426 mg (64%) of a colorless solid : [a] D²³ + 13.4 ° (c 0.407, MeOH); IR (KBr) 3260, 3150, 2980, 1779, 1727, 1649, 1573, 1547; ¹H NMR (CD3OD) d 7.58 (1H, s), 7.34-7.22 (5H, m), 4.59-4.47 (2H, m), 4.28 (1H, m), 4.07 (2H, s), 2.74-2.41 (2H, m), 1.42 (3H, d, J = 6.7); ¹³C NMR (CD3OD) d175.6, 175.5, 175.0,164.6, 164.5, 150.1, 138.7, 135.3.130.0, 129.9, 128.2, 122.9, 98 , 9, 98.5, 52.5, 52.2, 35.5, 35.1, 35.0, 19.0, 18.9. Analysis. Calculated for C18H20N4O5 H2O: C, 55.37; H, 5.68; N, 14.35. Found C, 55.83; H, 5.75; N, 13.96. MS (FAB, m / z): 373 (M⁺), 228, 185, 91.

<img file="PL193391B1_D0063.tif" />

PL 193 391 B1

5-Benzylpyrrole-2-carboxylic acid (34b). A mixture of ethyl 5-benzylpyrrole-2-carboxylate (0.7 g, 3.05 mmol; Elder et al., Synthetic Communications, 19, 763-767 (1989)), ethanol (20 ml) and 1M sodium hydroxide (9, 2 ml, 9.2 mmol) was stirred and refluxed for 3 hours. Most of the methanol was removed and the remaining liquid was diluted with water, washed with ether, chilled in ice and acidified with concentrated hydrochloric acid. The mixture was extracted with ether. The combined extracts were washed with brine, dried (Na2SO4), and concentrated to afford 0.567 g (92%) of an off-white solid; mp temp. 130-134 ° C;¹H NMR (CDCl3) d (1H, br s), 7.37-6.95 (5H, m), 6.97 (1H, m), 6.07 (1H, m), 4.00 (2H, s ).

(2R, S, 3S) N²- (pyrrole-2-carbonyl) -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (35a). A solution of (2R, S, 3S) N²-tert-butoxycarbonyl-N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (14) (756 mg, 2.0 mmol) in dry dichloromethane (8 ml) at 0 ° C treated with trifluoroacetic acid (8 ml) for 1 hour then evaporated to dryness. Dry ether was added to the residue, and the mixture was concentrated to a viscous oil. This oil was dissolved in dry DMF (10 ml). Pyrrole-2-carboxylic acid (34a) (244mg, 2.2mmol) was added and the solution cooled in an ice bath, followed by the addition of N, N-diisopropylamine (0.78g, 6.0mmol), hydroxybenzotriazole (0.1 54 g, 4.0 mmol) and dimethylaminopropylethylcarbodiimide hydrochloride (0.42 g, 2.2 mmol). The resulting mixture was stirred at 25 ° C for 17 hours, then a saturated aqueous sodium chloride solution (30 mL) was added. The mixture was extracted with ethyl acetate (3 x 20 ml) and the combined organic extracts washed with 5% aqueous, sodium bicarbonate solution (3 x 10 ml) and brine (10 ml), dried (MgSO4) and concentrated. Flash chromatography (25% hexane-ethyl acetate) gave 557 mg (75%) of a white glassy solid as a 1: 1: mp mixture of diastereomers. 85-90 ° C; IR (KBr) 3288, 1789, 1665, 1629, 1557, and 1122;¹H NMR (d6-DMSO) d 11.46 (1H, bs), 8.55 (0.5H, d, J = 7.0), 8.30 (0.5H, d, J = 7.6) , 8.06 (0.5H, d, J = 7.0), 8.04 (0.5H, d, J = 7.6), 7.36-7.30 (5H, m), 6. 88-6.85 (2H, m), 6.10-6.07 (1H, m), 5.63 (0.5H, d, J = 5.0), 5.42 (0.5H, s ), 4.72 (2H, q, J = 12.2), 4.74-4.25 (2H, m), 3.14-2.35 (2H, m), 1.29, 1.25 (3H, 2xd, J = 7.2).

(2R, S, 3S) N²- (5-benzylpyrrole-2-carbonyl) -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-alaninamide (35b) was prepared from 5-benzylpyrrole-2-carboxylic acid (34b) by the method described in for compound 35a (65%). Data are given for a single diastereomer.¹H NMR (d 6 -DMSO) d 11.37 (1H, br s), 8.27 (1H, d, J = 7.4), 7.93 (1H, d, J = 7.6), 7.33 -7.16 (10H, m), 6.76 (1H, m), 5.82 (1H, m), 5.62 (1H, d, J = 5.2), 4.76 (1H, d , J = 12.0), 4.65 (1H, m), 4.62 (1H, d, J = 12.2), 4.47 (1H, m), 3.88 (2H, s), 2.77 (1H, dd, J = 9.0, 18.0), 2.5 (dd), 1.23 (3H, d, J = 7.0).

(3S) 3- [N- (pyrrole-2-carbonyl) -L-alaninyl] amino-4-oxobutanoic acid (36a; D). A mixture of compound (35a) (612 mg, 1.65 mmol), methanol (40 ml) and 10% palladium on carbon (500 mg) was vigorously stirred under a hydrogen atmosphere for 4 hours. The mixture was filtered through 0.2 mM nylon membrane and then concentrated. The residue was purified by flash chromatography (5-10% methanol in methylene chloride) and precipitation from a mixture of ethyl acetate and ether gave hemihydrate of compound (36a) (223 mg, 48%) as a white solid. There were traces of a solvent in the product: mp. 96-100 ° C; IR (KBr) 3381, 1774, 1729 (EtOAc), 1632, 1558, 1523, 1123;¹H NMR (CD3OD) d 6.94-6.85 (2H, m), 6.17 (1H, dd, J = 3.8 and 2.6), 4.58 (0.5H, d, J = 3.94), 4.56 (0.5H, d, J = 4.24), 4.51 (1H, q, J = 7.16), 4.35-4.20 (1H, m), 2.74-2.40 (2H, m), 1.42 (3H, 2xd, J = 7.13).

(3S) 3- [N- (5-Benzylpyrrole-2-carbonyl) -L-alaninyl] amino-4-oxobutanoic acid (36b) was prepared (41%) from 35b as described for 36a to give an off-white solid: mp temp. 109-112 ° C; [a] D²⁵ + 6.3 ° (c 0.3, methanol); IR (KBr) 3368, 1724, 1630, 1530, 1453, 1414, 1233, 1049;¹H NMR (d4 methanol) d 7.25-7.11 (5H, m), 6.76 (1H, d, J = 3.5), 5.84 (1H, d, J = 3.5), 4.51 (1H, m), 4.43 (1H, q, J = 7.1), 4.23 (1H, m), 2.5 (2H, m), 1.35 (3H, d, J = 7.0). Analysis. Calculated for C19H21N3O5. 1.75H2O: C, 56.64; H, 6.13; N, 10.43. Found C, 56.34; H, 5.72; N, 10.00.

<img file="PL193391B1_D0064.tif" />

(2R, S, 3S) 1- (indole-2-carbonyl) -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (38). To a solution of (2R, S, 3S) 1-tert-butoxycarbonyl-N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (15) (0.607 g, 1.5 mmol) in dichloromethane (4 mL) at 0 &amp; lt; 0 &amp; gt; C was added trifluoroacetic acid (4 mL). The mixture was stirred at 0 ° C for 75 minutes. The mixture was concentrated and the residue was treated with diethyl ether and then the ether was removed in vacuo. This procedure was repeated twice to give a yellow oil which was dissolved in DMF (12 ml). Diisopropylethylamine (0.78 mL, 4.5 mmol) was added to the solution followed by indole-2-carboxylic acid (266 mg, 1.65 mmol), 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (316 mg, 1.65 mmol) and hydroxybenzotriazole (405 mg, 3 mmol). The mixture was stirred at room temperature for 20 hours then poured into brine. The mixture was extracted with ethyl acetate (3 x 30 ml). The combined organic extracts were washed with saturated aqueous sodium bicarbonate (2 x 60 mL) followed by brine (2 x 60 mL), dried (MgSO4) and concentrated. The residue was purified by column chromatography (ethyl acetate) to obtain 518 mg (77%) of a mixture of diastereomers: IR (KBr) 3314, 1780, 1677, 1609, 1524, 1435, 1406, 1344;¹H NMR (d6-DMSO) d 11.58 (1H, m), 8.81-8.41 (1H, m), 7.71-6.67 (10H, m), 5.70 (d, J = 5.2), 5.48 (s), 4.89-4.29 (4H, m), 3.99-3.74 (2H, m), 3.20-2.44 (2H, m ), 2.39-1.77 (4H, m).

(3S) 3- [1- (Indole-2-carbonyl) -L-prolinyl] amino-4-oxobutanoic acid (39). A mixture of (2R, S, 3S) 1- (indole-2-carbonyl) -N- (tetrahydro-2-benzyloxy-5-oxo-3-furanyl) -L-prolinamide (38) (478 mg, 1.07 mmol ), 10% palladium on carbon (475 mg) and methanol (150 ml) were stirred under a hydrogen atmosphere for 6 hours. The resulting mixture was filtered and concentrated to give a colorless glass. After recrystallization from a mixture of methanol and diethyl ether, 202 mg (53%) of a colorless glassy solid were obtained: mp. 135-138 ° C; [a] D²⁴ - 44 ° (0.25, CH 3 OH); IR (KBr) 3287, 2977, 2879, 1781, 1725, 1716, 1667, 1662, 1600, 1529, 1441, 1346;¹H NMR (CD3OD) d 7.65 (1H, d, J = 8.0), 7.44 (1H, d, J = 8.4), 7.22 (1H, m), 7.09-6 , 64 (2H, m), 4.62 (2H, m), 4.29 (1H, m), 4.15-3.73 (2H, m), 2.74-1.72 (6H, M ).

<img file="PL193391B1_D0065.tif" />

Methyl 2- (3,5-dihydro-7-methyl-4-oxo-4H-pyrrolo [3,2-d] pyrimidin-3-yl) acetate (40). To a solution of ethyl 3- [N- (dimethylamino) methylene) amino-4-methylpyrrole-2-carboxylate (1.56 g, 7.0 mmol;

Lim et al., J. Org. Chem., 44, pp. 3826-29 (1979)) in dry methanol (60 ml) was added with stirring freshly prepared methyl glycinate (1.25 g, 14 mmol). The resulting mixture was kept at 70 ° C. After 18 and 42 hours of heating, two portions of methyl glycinate (1.25, 14.0 mmol) were added. The mixture was cooled and filtered 24 hours after the last addition. The filtrate was concentrated and the residue was purified by flash chromatography (2-5% methanol / chloroform) to afford 0.54 g (35%) of a white crystalline solid: mp. 233-235 ° C (recrystallized from ethyl acetate); IR (KBr) 3135, 2958, 1745, 1675, 1254;¹H NMR (d 6 -DMSO) d 11.90 (1H, s), 8.07 (1H, s), 7.23 (1H, s), 4.83 (2H, s), 3.69 (3H, s), 2.16 (3H, s). Analysis. Calculated for C10H11N3O3 0.1H2O: C, 53.85; H, 5.07; N, 18.84. Found C, 53.85; H, 4.96; N, 18.81; MS (70 eVe.I) m / e 222,221 (M⁺, 100%), 189, 162, 133, 105.

2- (3,5-Dihydro-7-methyl-4-oxo-4H-pyrrolo [3,2-d] pyrimidin-3-yl) -acetic acid sodium salt (41). A suspension of compound 40 (354 mg, 1.6 mmol) in methanol (15 mL) was treated with 0.5 N dil.

With sodium hydroxide solution (4.8 ml) and the resulting mixture was stirred at 25 ° C for 1 hour. The reaction mixture was filtered to provide compound 41 hemihydrate (354 mg, 97%) as a white crystalline solid: mp. &gt; 340 ° C (recrystallized from methanol); IR (KBr) 3461, 3143, 1676, 1666, 1605, 1415;¹H NMR (d 6 -DMSO) d 11.63 (1H, s), 7.83 (1H, s), 7.11 (1H, d, J = 2.0), 4.24 (2H, s), 2.14 (3H, s). Analysis. Calculated for C9H8N3O3Na. 0.5H2O: C, 45.39; H, 3.81; N, 17.64. Found C, 45.57; H, 4.05; N, 17.39.

(2R, S, 3S) 2- (3,5-dihydro-7-methyl-4-oxo-4H-pyrrolo [3,2-d] pyrimidin-3-yl) -N- (tetrahydro-2-benzyloxy- 5-oxo-3-furanyl) acetamide (42). A suspension of compound 41 sodium (344 mg, 1.5 mmol) in dry DMF (15 mL) was treated with ethyldimethylaminopropylcarbodiimide hydrochloride (373 mg, 1.95 mmol) and 1-hydroxybenzotriazole (405 mg, 3.0 mmol). The mixture was kept at 25 ° C for 1 hour then (2R, S, 3S) N-allyloxycarbonyl-3-amino-2-benzyloxy-5-oxotetrahydrofuran (437 mg, 1.5 mmol; Chapnam, Biorg. Med. Chem. Lett., 2, pp. 613-618 (1992)) and (Ph₃P)₂PdCl₂ (25 mg) then n-tributyltin hydride (0.6 mL, 2.25 mmol) was added dropwise. The resulting mixture was stirred at 25 ° C for 1 hour, then water (20 mL) was added. The mixture was extracted with ethyl acetate (3 x 15 ml) and the combined organic extracts were washed with water (5 ml), dried (MgSO4) and concentrated to give a mixture of diastereomers. Evaporation of the aqueous phase and purification of the residue by flash chromatography (5% methanol / chloroform) gave additional compound for a total of 182 mg of compound 42 (31%); mp temp. 240-242 ° C; IR (KBr) 3274, 1772, 1691, 1664, 1562;¹H NMR (d6-DMSO) d 11.81 (1H, s), 8.85 (0.6H, d, J = 6.6), 8.72 (0.4H, d, J = 7.4) , 7.98 (0.6H, s), 7.95 (0.4H, s), 7.40-7.30 (5H, m), 7.20 (1H, d, J = 2.2) , 5.61 (0.4H, d, J = 7.5), 5.46 (s), 4.85-4.60 (m), 4.28 (m), 3.20-2.35 (2H, m), 2.16 (3H, s).

(3S) -3- [2- (3,5-Dihydro-7-methyl-4-oxo-4H-pyrrolo [3,2-d] pyrimidin-3-yl) -1-oxo-ethylamino] -4 acid -oxobutane (43). A mixture of compound 42 (131 mg, 0.33 mmol) in methanol (50 ml) and 10% palladium on carbon (100 mg) was vigorously stirred under a hydrogen atmosphere for 2 hours. More catalyst (100 mg) was added and the mixture was hydrogenated for an additional 2 hours. The mixture was filtered through 0.2 mM nylon membrane and concentrated. The residue was recrystallized from methanol and diethyl ether to give 79 mg (78%) of compound 43 as a hygroscopic white solid; mp temp. 222-226 ° C (decomposition); [a] D³²+ 0.5 ° (c 0.02, MeOH); IR (KBr) 3282, 1680, 1558, 1425, 1275;¹H NMR (CD3OD) d 8.03 (1H, s), 7.18 (1H, d, J = 0.7), 4.79-4.74 (2H, m), 4.63-4.59 (1H, 2xd, J = 3.6), 4.36-4.25 (1H, m), 2.78-2.39 (2H, m), 2.24 (3H, d, J = 0 , 7). Analysis. Calculated for C13H14N4O5Na. 1.4H2O: C, 47.10; H, 5.12; N, 16.90. Found C, 47.00; H, 4.79; N, 16.59. FABMS m / e 307, 306 (M⁺), 244, 207, 190, 152, 115 (100%).

<img file="PL193391B1_D0066.tif" />

(a) X = O (b) X = H.₂

PL 193 391 B1 (1S, 9S) t-butyl 6,10-dioxo-octahydro-9- (3-phenylpropionylamino) -6H-pyridazine [1,2-a] [1,2] diazepine-1-carboxylate (44a ). To a solution of t-butyl (1S, 9S) 9-amino-6,10-dioxo-octahydro-6H-pyridazine [1,2-a] [1,2] diazepine-1-carboxylate (690 mg, 2.32 mmol ; GB 2128984) in dioxane (16 ml) and water (4 ml) was added solid sodium bicarbonate (292 mg, 3.48 mmol) at 0 ° C followed by dropwise addition of 3-phenylpropionyl chloride (470 mg, 2.78 mmol). ). The mixture was stirred at room temperature for 2 hours then additional sodium bicarbonate (200 mg; 2.38 mmol) and 3-phenylpropionyl chloride (100 mg, 0.6 mmol) were added. The mixture was stirred at room temperature for an additional 2 hours, diluted with ethyl acetate (50 ml), washed with saturated sodium bicarbonate solution (2 x 25) then dried (MgSO 4) and concentrated. The residue was purified by flash chromatography (0-50% ethyl acetate / chloroform) and finally crystallized by trituration with ether to afford 860 mg (86%) of a white solid: mp. 137-138 ° C; [a] D²³-95.1 ° (c 0.549, CH2Cl2); IR (KBr) 3327, 1736, 1677, 1664, 1536, 1422, 1156;¹H NMR (CDCl3) d 7.24 (5H, m), 6.50 (1H, d, J = 7.5), 5.24 (1H, m), 4.90 (1H, m), 4.60 (1H, m), 3.44 (1H, m), 2.93 (2H, m), 2.84 (1H, m), 2.64 (1H, m), 2.54 (2H, m) , 2.26 (2H, m), 1.70 (4H, m), 1.70 (9H, s). MS (FAB, m / z): 430 (M.⁺+1), 374, 242, 105, 91.

T-Butyl (1S, 9S) octahydro-10-oxo-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxylate (44b) was prepared from (1S , 9S) t-butyl 9-amino-octahydro-10-oxo-6H-pyridazine [1,2-a] [1,2] diazepine-1-carboxylates (Attwood et al., J. Chem. Soc. Perkin 1, pp. 1011-19 (1986)) like compound 44a to give 810 mg (81%) as a colorless oil; [a] D²³ -33.5 ° (c 0.545, CH₂Cl₂); IR (KBr) 3334, 2935, 1737, 1728, 1659, 1642;¹H NMR (CDCl3) d 7.24 (5H, m), 6.75 (1H, d, J = 6.7), 5.27 (1H, m), 4.92 (1H, m), 3. 39 (1H, m), 3.03 (4H, m), 2.55 (3H, m), 2.33 (1H, m), 2.17 (1H, m), 1.80 (5H, m) ), 1.47 (9H, s), 1.39 (1H, m). MS (FAB, m / z): 416 (M.⁺+ 1), 360, 211, 143, 97.

(1S, 9S) 6,10-Dioxo-octahydro-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxylic acid (45a). To a solution of (1S, 9S) t-butyl 6,10-dioxo-octahydro-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxylate (44a) (800 mg, 1.863 mmol) in dry dichloromethane (5 mL) was added trifluoroacetic acid (5 mL) at 0 ° C. The solution was stirred at room temperature for 3 hours then concentrated. Dry ether (10 mL) was added to the residue then removed in vacuo. This operation was repeated three times to obtain a crystalline solid. This material was triturated with ether and filtered to give 590 mg (85%) of a white crystalline solid: mp. 196-197.5 ° C; [a] D²³ -129.5 ° (c 0.2, CH 3 OH); IR (KBr) 3237, 1688, 1660, 1633, 1574, 1432, 1285, 1205;¹H NMR (CD3OD) d 8.28 (1H, d, J = 7.4), 7.22 (5H, m), 5.32 (1H, dd, J = 5.9, 2.9), 4. 75 (1H, m), 4.51 (1H, m), 3.50 (1H, m), 3.01 (1H, m), 2.91 (2H, m), 2.55 (2H, m ), 2.29 (3H, m), 1.95 (2H, m), 1.71 (2H, m). Analysis. Calculated for C19H23N3O5: C, 61.12; H, 6.21; N, 11.25. Found C, 60.80; H, 6.28; N, 10.97. MS (FAB, m / z) 374 (M.⁺+ 1), 242, 105, 91.

(1S, 9S) octahydro-10-oxo-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxylic acid (45b) was prepared from octahydro-10- t-butyl oxo-9- (3-phenylpropionylamino) -6H-pyridazine [1,2-a] [1,2] diazepine-1-carboxylate (44b) as described for 45a gave 657 mg (96%) compound 45b as a crystalline solid: mp. 198-202 ° C; [a] D²³ -86.2 ° (c 0.5, CH 3 OH); IR (KBr) 3294, 2939, 1729, 1645, 1620, 1574, 1453, 1214;¹H NMR (CD3OD) d7.92 (1H, d, J = 7.9), 7.20 (5H, m), 5.29 (1H, m), 4.90 (1H, m), 3.47 (1H, m), 3.08 (2H, m), 2.90 (2H, m), 2.55 (3H, m), 2.36 (1H, m), 1.81 (5H, m) , 1.43 (2H, m). MS (FAB, m / z) 360 (M.⁺+ 1), 211, 143, 91.

[3S, 2R, S, (1S, 9S)] N- (2-benzyloxy-5-oxotetrahydrofuran-3-yl) -6.10-dioxo-octahydro-9- (3-phenylpropionylamino) -6H-pyridazine- [ 1,2-a] [1,2] diazepine-1-carboxamide (46a). For a solution of (1S, 9S) 6,10-dioxo-octahydro-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] -diazepine-1-carboxylic acid (45a) ( 662 mg, 1.773 mmol) in dry dichloromethane (9 ml) and dry dimethylformamide (3 ml) at room temperature were added bis (triphenylphosphine) palladium chloride (30 mg) and (3S, 2R, S) -3-allyloxycarbonylamino-2-benzyloxy -5-oxotetrahydrofuran (Chapnam, Biorg. Med. Chem. Lett :, 2, p. 613-618 (1992)), then tri-n-butyltin hydride (1.19 g, 4.09 mmol) was added dropwise. 1-Hydroxy-benzotriazole (479 mg, 3.546 mmol) was added to the mixture and the mixture was cooled to 0 ° C, then 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (408 mg, 2.128 mmol) was added. The mixture was stirred at room temperature for 3.25 hours then diluted with ethyl acetate (50 ml), washed twice with dilute hydrochloric acid (20 ml), twice with saturated sodium bicarbonate solution (20 ml), once with brine then dried (MgSO4). and concentrated. The resulting oil was purified by flash chromatography (0-100% ethyl acetate / chloroform) to afford 810 mg (81%) of 46a as a mixture of anomers: mp. 92-94 ° C; IR (KBr) 3311, 1791, 1659, 1651, 1536;¹H NMR (CDCl3) d 7.49, 6.56 (1H, 2d, J = 6.7, 7.8),

PL 193 391 B1

7.29 (10H, m), 6.37, 6.18 (1H, 2d, J = 7.7, 7.6), 5.56, 5.34 (1H, d, s, J = 5, 2), 5.08-4.47 (6H), 3.18-2.80 (5H), 2.62-2.28 (5H), 2.04-1.53 ​​(5H). MS (FAB, m / z) 563 (M.⁺ + 1), 328, 149, 91.

[3S, 2R, S, (1S, 9S)] N- (2-benzyloxy-5-oxotetrahydrofuran-3-yl) -octahydro-10-oxo-9- (3-phenylpropionylamino) -6H-pyridazine- [1, 2-a] [1,2] diazepine-1-carboxamide (46b) was prepared from 45b as described for 46a to give 790 mg (96%) of a glass: mp. 58-60 ° C; IR (KBr) 3316, 2940, 1793, 1678, 1641, 1523, 1453, 1120;¹H NMR (CDCl3) d 7.28 (10H, m), 6.52, 6.42 (1H, 2d, J = 7.2, 7.1), 5.53, 5.44 (1H, d, s, J = 5.2), 5.35 (1H, m), 4.34 (4H, m), 3.1-2.8 (6H, m), 2.6-2.1 (7H) , 1.95-1.05 (5H). MS (FAB, m / z) 549 (M⁺ + 1), 400, 310, 279, 91.

Acid [3S, (1S, 9S)] 3- (6,10-dioxo-octahydro-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxamido ) -4-oxobutane (47a). A mixture of [3S, 2R, S, (1S, 9S)] N- (2-benzyloxy-5-oxotetrahydrofuran-3-yl) -6.10-dioxo-octahydro-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxamide (46a) (205 mg; 0.364 mmol), 10% palladium on carbon (200 mg) and methanol (20 mL) was stirred under an atmospheric pressure of hydrogen for 5 hours. The mixture was filtered and then concentrated to give 154 mg (90%) of a glass: mp. 116-118 ° C; [a] D²³ -140 ° (c 0.1, CH 3 OH); IR (KBr) 3323 (br), 1783, 1731, 1658, 1539, 1455, 1425;¹H NMR (CD 3 OD) d 7.21 (5H, m), 5.17 (1H, m), 4.73 (1H, m), 4.50 (2H, m), 4.23 (1H, m) , 3.38 (1H, m), 3.06 (1H, m), 2.91 (2H, m), 2.73-2.18 (6H, m), and 2.01-1.59 (5H , m). Analysis. Calculated for C23H27N4O7 + H2O: C, 56.32; H, 6.16; N, 11.42. Found C, 56.29; H, 6.11; N, 11.25. MS (FAB, m / z) 473 (M.⁺ + 1), 176, 149, 105, 91.

Acid [3S, (1S, 9S)] 3- (octahydro-10-oxo-9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxamido) - 4-Oxobutane (47b) was prepared from 46a by the method described for 47a. The residue was purified by flash chromatography (0-10% methanol / chloroform) to give 65 mg (52%) of a glass: mp. 87-90 ° C; [a] D²³ -167.0 ° (c 0.1, methanol); IR (KBr) 3329, 2936, 1786, 1727, 1637;¹H NMR (CD 3 OD) d 7.23 (5H, m), 5.29 (1H, m), 4.83 (1H, m), 4.59 (1H, d, J = 3.6), 4. 29 (1H, m), 3.3-3.0 (3H, m), 2.91 (2H, m), 2.70-2.34 (5H, m), 2.19 (2H, m) , 1.75 (4H, m), 1.36 (2H, m). Analysis. Calculated for C23H30N4O6 + 0.5H2O: C, 59.09; H, 6.68; N, 11.98. Found C, 58.97; H, 6.68; N, 11.73. MS (FAB, m / z) 459 (M.⁺ + 1), 310, 149, 105, 91.

<img file="PL193391B1_D0067.tif" />

<td colspan="2">Ri</td><td> 1½</td><td>r₃</td>

<td>(and)</td><td>PhCH₂</td><td>H.</td><td>(S) Me</td>

<td>(b)</td><td>PhCH₂</td><td>CH₂Ph</td><td>H.</td>

<td>(c)</td><td>PhCH₂</td><td>(CH₂)₂Ph</td><td>H.</td>

<td>(d)</td><td>PhCH₂</td><td>nBu</td><td>H.</td>

<td>(e)</td><td>PhCH₂</td><td>Me</td><td>H.</td>

<td>(f)</td><td>PhCH₂</td><td>Ph</td><td>H.</td>

<td>(g)</td><td>PhCH₂</td><td>H.</td><td>H.</td>

<td>(h)</td><td>PhCH₂</td><td>CH₂Ph</td><td>(S) -Me</td>

<td>(and)</td><td>Ph (CH₂)₂</td><td>CH₂Ph</td><td>H.</td>

PL 193 391 B1

Pyridones 48 were prepared as described by Damewood et al., J. Med. Chem., 37, pp. 3303-12 (1994)). Compound 48d is new.

3-benzyloxycarbonylamino-6-butyl-pyrid-2-one (48d) was isolated as a cream solid: mp. 158-160 ° C; IR (KBr) 3382, 2953, 2930, 2866, 1729, 1643, 1524, 1468, 1202, 1044.¹H NMR (d 6 -DMSO) d 8.26 (1H, s), 7.72 (1H, d), 7.39 (5H, m), 6.00 (1H, d), 5.14 (2H, s), 2.41 (2H, t), 1.52 (2H, m), 1.24 (2H, m), 0.87 (3H, t). Analysis. Calculated for C17H20N2O3: C, 67.98;

H, 6.71; N, 9.33. Found C, 67.69; H, 6.68; N, 9.20. MS CI M⁺ = 300 (m)) 28%.

Methyl (2S) 2- [3-benzyloxycarbonylamino-1,2-dihydro-2-oxo-1-pyridyl] propionate (49a). Sodium hydride (80% oil dispersion) (0.35 g) was added to a mixture of 3- (benzyloxycarbonylamino) pyrid-2-one (48a) (2.58 g, 10.58 mmol) and tetrahydrofuran (100 ml) at room temperature. , 11.64 mmol). The mixture was stirred for 10 minutes. To a solution of methyl 2 (R) 2 - ((trifluoromethane) sulfonyloxy) propionate (2.5 g, 10.58 mmol; Feenstra et al. Tetrahedron Lett., 28, p. 1215-18 (1987)) in tetrahydrofuran (5 ml) at room temperature was added the solution prepared above in 10 minutes. The mixture was stirred at room temperature for 80 minutes then poured into ethyl acetate. The mixture was washed twice with 1M HCl, twice with aqueous sodium bicarbonate, then with brine, then dried (MgSO4) and concentrated. The residue was purified by flash chromatography (30% ethyl acetate / hexane) to afford 2.945 g (84%) of a colorless solid: mp. 96-7 ° C; [a] D²⁰ -71.36 (c 2.5, CHCl2); IR (KBr) 3370, 1764, 1729, 1648, 1602, 1564, 1523, 1515, 1503, 1449, 1359, 1203, 1064;¹H NMR (CDCl3) d 8.04 (1H, d, J = 7.2), 7.86 (1H, s), 7.36 (5H, m), 6.98 (1H, dd, J = 7 , 1, J = 7.1), 6.30 (1H, t, J = 7.2), 5.46 (1H, q, J = 7.4), 5.20 (2H, s), 3 . 74 (3H, s).

1.66 (3H, d, J = 7.4). Analysis. Calculated for C17H18N2O5: C, 61.81; H, 5.49; N, 8.48. Found C, 61.49; H, 5.51; N, 8.41. MS (FAB, m / z) 331 (M⁺ +1), 299, 223, 196, 163, 91.

Methyl [6-benzyl-3-benzyloxycarbonylamino-1,2-dihydro-2-oxo-1-pyridyl] acetate (49b).

Sodium hydride (80% oil dispersion) was added to a mixture of 6-benzyl-3- (benzyloxycarbonylamino) pyrid-2-one (48b) (7.3 g, 2.18 mmol) and tetrahydrofuran (150 ml) at room temperature ( 0.65 g, 26.2 mmol). The mixture was stirred for 10 minutes, treated with methyl bromoacetate (2.5 mL, 26.2 mmol) and left for 3 hours. The resulting mixture was poured into a mixture of ice and 1M HCl. The solid formed was filtered off and then dissolved in dichloromethane. The resulting solution was dried (MgSO4), decolorized with charcoal and concentrated. The residue was purified by chromatography (2-5% ethyl acetate / dichloromethane) to give 7.2 g (81%) of colorless crystals: mp. 117-9 °; IR (KBr) 3375, 1753, 1730, 1651, 1605, 1513, 1384, 1223, 1185, 1071;¹H NMR (CDCl3) d 8.02 (1H, d, J = 7.5), 7.78 (1H, s), 7.31 (8H, m), 7.10 (2H, m), 6. 15 (1H, d, J = 7.45), 5.20 (2H, s), 4.70 (2H, s), 3.88 (2H, s), 3.66 (3H, s).

The following compounds were prepared in a similar manner:

Methyl [3-benzyloxycarbonylamino-1,2-dihydro-2-oxo-6-phenethyl-1-pyridyl] -acetate (49c).

97% yield; mp temp. 102-4 ° C. IR (KBr) 3245, 2323, 1741, 1725, 1648, 1600, 1526, 1216;¹H NMR (d 6 -DMSO) d 8.45 (1H, s), 7.76 (1H, d, J = 7.6), 7.35 (10H, m), 6.15 (1H, d, J = 7.6), 5.15 (2H, s), 4.85 (2H, s), 3.68 (3H, s), 2.86 (4H, s).

Methyl [3-benzyloxycarbonylamino-6-butyl-1,2-dihydro-2-oxo-1-pyridyl] -acetate (49d). 90% yield; mp temp. 112 ° C. IR (KBr) 3393, 1738, 1731, 1645, 1598, 1517, 1225, 1208;¹H NMR (d 6 -DMSO) d 8.39 (1H, s), 7.78 (1H, d, J = 7.7), 7.35 (5H, m), 6.17 (1H, d, J = 7.7), 5.15 (2H, s), 4.80 (2H, s), 3.67 (3H, s), 1.38 (6H, m), 0.89 (3H, t) .

Methyl [3-benzyloxycarbonylamino-1,2-dihydro-6-methyl-2-oxo-1-pyridyl] -acetate (49e).

Yield 84% as a colorless solid: mp. 115-6 °; IR (KBr) 3246, 1740, 1725, 1649, 1598, 1535, 1417, 1365, 1259, 1219, 1193,¹H NMR (d 6 -DMSO) d 8.40 (1H, s), 7.75 (1H, d, J = 7.6), 7.38 (5H, m), 6.20 (1H, d, J = 7.6), 5.15 (2H, s), 4.85 (2H, s), 3.68 (3H, s), 2.26 (3H, s).

Methyl [3-benzyloxycarbonylamino-1,2-dihydro-6-phenyl-1-pyridyl] -acetate (49f). Yield 67% as a colorless oil: IR (KBr) 3266, 1739, 1727, 1646, 1606, 1566, 1517, 1490, 1365, 1213, 1163, 1075;¹H NMR (CDCl3) d 8.16 (1H, d), 7.85 (1H, s), 7.39 (10H, m), 6.22 (1H, d), 5.22 (2H, s) , 4.57 (2H, s), 3.74 (3H, s).

Methyl [3-benzyloxycarbonylamino-1,2-dihydro-2-oxo-1-pyridyl] acetate (49g). Yield 80% in the form of a colorless crystalline substance: mp. 110-111 ° C. IR (KBr) 3385, 1745, 1650, 1601, 1512, 1502, 1378, 1369, 1358, 1215, 1195, 1162, 1067;¹H NMR (CDCl) d 8.06 (1H, d), 7.84 (1H, s), 7.36 (5H, m), 6.88 (1H, dd), 6.27 (1H, t ), 5.20 (2H, s), 4.68 (2H, s), 3.78 (3H, s). Analysis. Calculated for C16H16N2O5: C, 60.75; H, 5.10; N, 8.85. Found C, 60.55; H, 5.15; N, 8.85. MS FAB (+) M + = 317 (M +1).

PL 193 391 B1

Methyl 2-methyl- [6-benzyl- (3-benzyloxycarbonylamino) -1,2-dihydro-2-oxo-1-pyridyl] acetate (49h) was prepared by the method used to prepare 49a to give an oil (58%); [a] D²⁵ -25.0 ° (c 1, CH2Cl2); IR (KBr) 3381, 1736, 1650, 1604, 1513, 1218, 1190, 1068;¹H NMR (CDCl3) d 7.97 (1H, d), 7.78 (1H, s), 7.4-7.14 (10H, m), 6.17 (1H, d), 5.19 ( 2H, s), 4.64 (1H, q), 3.98 (2H, s), 3.62 (3H, s), 1.31 (3H, d).

Methyl [6-benzyl-1,2-dihydro-2-oxo-3- (2-phenylethoxy) carbonylamino-1-pyridyl] acetate (49i) was prepared (88%) as a colorless solid: mp. 130-133 ° C; IR (KBr) 3363, 1746, 1732, 1651, 1604, 1515, 1368, 1231, 1212, 1185;¹H NMR (CDCl3) d 8.00 (1H, d, J = 7.0), 7.68 (1H, s), 7.36-7.10 (10H, m), 6.15 (1H, d , J = 7.6), 4.7 (2H, s), 4.38 (2H, t, J = 7.0), 3.88 (2H, s), 3.67 (3H, s), 2.98 (2H, t, J = 7).

<img file="PL193391B1_D0068.tif" />

Methyl 2 (S) 2 [3-amino-1,2-dihydro-2-oxo-1-pyridyl] propionate (50a). A mixture of methyl 2 (S) -2 [3-benzyloxycarbonylamino-1,2-dihydro-2-oxo-1-pyridyl] propionate (49a) (2.75 g, 8.33 mmol), methanol (100 ml) and 10 % palladium on carbon (300 mg) was stirred under a hydrogen atmosphere for 30 minutes. The mixture was filtered and concentrated to give 1.63 g (100%) of a colorless solid:¹H NMR (d 6 -DMSO) d 8.35 (1H, br s), 7.46 (1H, d), 7.22 (1H, d), 6.29 (1H, t), 5.22 (1H, q), 3.63 (3H, s), 1.55 (3H, d).

The following compounds were prepared in a similar manner:

Methyl [3-amino-6-benzyl-1,2-dihydro-2-oxo-1-pyridyl] acetate (50b). Yield: 100% as a gray solid: mp. 134-6 ° C; IR (KBr) 3418, 3312, 1723, 1658, 1596, 1548, 1435, 1290, 1245, 1011;¹H NMR (d 6 -DMSO) d 7.25 (5H, m), 6.45 (1H, d, J = 7.4), 5.92 (1H, d, J = 7.4), 5.00 (2H, s), 4.63 (2H, s), 3.88 (2H, s), 3.51 (3H, s).

Methyl 3-amino-1,2-dihydro-2-oxo-6-phenethyl-1-pyridyl] acetate (50c). Yield: 99% as a viscous oil: IR (KBr) 3456, 341, 2953, 1745, 1649, 1600, 1548, 1219;¹H NMR (CDCl3) d 7.25 (5H, m), 6.51 (1H, d, J = 7.4), 5.92 (1H, d, J = 7.4), 4.79 (2H , s), 3.77 (3H, s), 2.80 (4H, m).

Methyl [3-amino-6-butyl-1,2-dihydro-2-oxo-1-pyridyl] acetate (50d). Yield: 97% as a brown solid: mp. 75-7 ° C; IR (KBr) 3437, 3342, 2955, 1745, 1655, 1609, 1550, 1432, 1301, 1222, 1200;¹H NMR (CDCl3) d 6.53 (1H, d, J = 6.8), 5.93 (1H, d, J = 6.8), 4.81 (2H, s), 3.77 (3H , s), 2.44 (2H, t), 1.45 (4H, m), 0.93 (3H, t).

Methyl [3-amino-1,2-dihydro-6-methyl-2-oxo-1-pyridyl] acetate (50e) was isolated (100%) as a colorless crystalline solid: mp. 87-9 ° C; IR (KBr) 3442, 3326, 1735, 1647, 1600, 1549, 1434, 1407, 1383, 1366, 1225, 1209;¹H NMR (d6-DMSO) d 6.40 (1H, d, J = 7.3), 5.93 (1H, d, J = 7.3), 4.86 (2H, s), 4.79 (2H, s), 3.67 (3H, s), 2.15 (3H, s).

Methyl [3-amino-1,2-dihydro-2-oxo-6-phenyl-1-pyridyl] acetate (50e) was isolated (86%) as a gray solid: mp. 207-9 ° C; IR (KBr) 3473, 3345, 1750,

1644, 1600, 1536, 1443, 1336, 1309, 1212, 1184, 1156; ¹H NMR (d-DMSO) d 7.30 (5H, m), 6.54 (1H, d), 6.03 (1H, d), 5.25 (2H, s), 4.49 (2H, s), 3.61 (3H, s).

Methyl [3-amino-1,2-dihydro-2-oxo-1-pyridyl] acetate (50g) was obtained as a colorless oil and used directly in the next step.

Methyl (2S) 2-methyl- [3-amino-6-benzyl-1,2-dihydro-2-oxo-1-pyridyl] acetate (50h) isolated (58%) as a colorless oil: IR (shell) 3354 , 1743, 1646, 1600, 1548, 1494, 1309, 1268, 1227, 113; ¹H NMR (C6D6) d 7.29-6.76 (5H, m), 5.86 (1H, d, J = 7.2), 5.51 (1H, d, J = 7.2), 4 , 43 (1H, q, J = 6.7), 3.69 (2H, s), 3.21 (2H, s), 3.36 (3H, s), 1.43 (3H, d, J = 6.7).

<img file="PL193391B1_D0069.tif" />

PL 193 391 B1

<td></td><td>R1</td><td>R2</td><td>R3</td>

<td>(and)</td><td>Ph (CH2) 2CO</td><td>H.</td><td>(S) Me</td>

<td>(b)</td><td>Ph (CH2) 2CO</td><td>CH2Ph</td><td>H.</td>

<td>(c)</td><td>Ph (CH2) 2CO</td><td>(CH2) 2Ph</td><td>H.</td>

<td>(d)</td><td>Ph (CH2) 2CO</td><td>nBu</td><td>H.</td>

<td>(e)</td><td>Ph (CH2) 2CO</td><td>Me</td><td>H.</td>

<td>(f)</td><td>Ph (CH2) 2CO</td><td>Ph</td><td>H.</td>

<td>(g)</td><td>Ph (CH2) 2CO</td><td>H.</td><td>H.</td>

<td>(h)</td><td>Ph (CH2) 2CO</td><td>CH2Ph</td><td>(S) -Me or (R, S) -Me</td>

<td>(and)</td><td>AcTyr</td><td>CH2Ph</td><td>H.</td>

<td>(j)</td><td>Ph (CH2) 2SO2</td><td>CH2Ph</td><td>H.</td>

<td>(k)</td><td>Ph (CH2) 2OCO</td><td>CH2Ph</td><td>H.</td>

<td>(l)</td><td>Ph (CH2) 3CO</td><td>CH2Ph</td><td>H.</td>

Methyl 2 (S) 2- [1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] propionate (51a).

To a mixture of methyl 2S 2- [3-amino-1,2-dihydro-2-oxo-1-pyridyl] propionate (50a) (1.63 g, 8.33 mmol), dioxane (60 ml), water (15 ml) and sodium bicarbonate (1.54 g, 16.7 mmol) were added dropwise with stirring 3-phenylpropionyl chloride (1.5 g, 9 mmol). The mixture was left for 1 hour and then extracted with ethyl acetate. The extracts were washed with aqueous sodium bicarbonate, dried (MgSO4) and concentrated. The resulting red oil was purified by flash chromatography to give 2.54 g (93%) of an oil: [a] D²⁰ -68 ° (1, CH2Cl2); IR (CH2Cl2) 3369, 1747, 1690, 1650, 1602, 1512, 1267, 1260, 1217;¹H NMR (CDCl3) d 8.41 (1H, dd), 8.36 (1H, s), 7.24 (5H, m), 7.02 (1H, dd), 6.32 (1H, t), 5.44 (1H, q), 3.75 (3H, s), 3.03 (2H, t), 2.70 (2H, t), 1.66 (3H, d). FAB M + = 329 (M + 1), 197, 165, 131, 110, 91.

The following compounds were prepared in a similar manner:

Methyl [6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -acetate (51b) was isolated (93%) as crystals: mp. 95-7 °; IR (KBr) 3265, 1747, 1686, 1642, 1590, 1563, 1511, 1454, 1401, 1220, 1183, 1133;¹H NMR (CDCl3) d 8.39 (1H, d, J = 7.7), 8.27 (1H, s), 7.21 (10H, m), 6.17 (1H, d, J = 7, 7), 4.70 (2H, s), 3.89 (2H, s), 3.67 (3H, s), 3.02 (2H, m), 2.70 (2H, m).

Methyl [1,2-dihydro-2-oxo-6-phenethyl-3- (3-phenylpropionyl) amino-1-pyridyl] -acetate (51c) was isolated (81%) as colorless crystals: m.p. 105-8 ° C; IR (KBr) 3378, 1746, 1680, 1646, 1597, 1517, 1221;¹H NMR (CDCl3) d 8.34 (1H, d, J = 7.7), 8.25 (1H, s), 7.23 (10H, m), 6.11 (1H, d, J = 7 , 7), 4.77 (2H, s), 3.78 (3H, s), 2.88 (8H, m).

Methyl [6-butyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -acetate (51d) was isolated (88%) as colorless crystals: mp. 84-5 ° C; IR (KBr) 3345, 2958, 2930, 1756, 1693, 1650, 1602, 1510, 1227, 1180, 1137;¹H NMR (CDCl3) d 8.34 (1H, d, J = 7.7), 8.22 (1H, s), 7.26 (5H, m), 6.12 (1H, d, J = 7, 7), 4.80 (2H, s), 3.79 (3H, s), 3.03 (2H, t), 2.68 (2H, t), 2.50 (2H, t), 1. 46 (4H, m), 0.95 (3H, t).

Methyl [1,2-dihydro-6-methyl-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -acetate (51e) was isolated (100%) as a pale yellow oil: IR (shell) 3264. 1745, 1691, 1644, 1587, 1566,

1518, 1495, 1400, 1215, 1183, 1136; ¹H NMR (CDCl3) d 8.33 (1H, d, J = 7.6), 7.26 (5H, m), 6.13 (1H, d, J = 7.6), 4.83 (2H, s), 3.79 (3H, s), 3.03 (2H, m), 2.69 (2H, m), 2.28 (3H, s).

Methyl [1,2-dihydro-2-oxo-6-phenyl-3- (3-phenylpropionyl) amino-1-pyridyl] -acetate (51f) was isolated (99%) as a pale yellow oil: IR (shell) 3365, 3299, 1751, 1689, 1643, 1600, 1563,

1519, 1493, 1419, 1370, 1224; ¹H NMR (CDCl3) d 8.46 (1H, d, J = 7.7), 8.32 (1H, s), 7.32 (10H, m), 6.24 (2H, d, J = 7, 7), 4.57 (2H, s), 3.73 (3H, s), 3.06 (2H, s), 2.72 (2H, m).

Methyl [1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -acetate (51f) was isolated (81%) as an oil: IR (shell) 3330, 1753, 1689, 1650 , 1600, 1560, 1517, 1374, 1225, 1208; ¹HNMR (CDCl3) d 8.43 (1H, dd, J = 7.4, 1.7), 8.33 (1H, s), 7.28 (5H, m), 6.92 (1H, dd, J = 6.9, 1.7),

6.29 (1H, t), 4.67 (2H, s), 3.79 (3H, s), 3.04 (2H, m), 2.70 (2H, m). MS FAB (+) M + = 315 (M + 1).

Methyl 2 (S) 2-methyl- [6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -acetate (51g) was isolated (93%) as a colorless oil; [a] D³⁰ - 19 ° (c 1, CH2Cl2); IR (coating) 3354, 3313, 3028, 2950, ​​1745, 1687, 1645, 1600, 1567, 1514, 1454, 1225;¹H NMR (CDCl3) d 8.35 (1H, d, J = 7.5), 8.26 (1H, s), 7.27 (10H, m), 6.20 (1H, d, J = 7 5), 4.65 (1H, q, J = 6.8), 3.99 (2H, s), 3.71 (3H, s), 3.03 (2H, m), 2.68 ( 2H, m), 1.31 (3H, d, J = 6.8).

PL 193 391 B1

Methyl [3- (N-acetyl-O-benzyl-L-tyrosine) amino-6-benzyl-1,2-dihydro-2-oxo-pyridyl] acetate (51i). A mixture of methyl [3-amino-6-benzyl-1,2-dihydro-2-oxo-1-pyridyl] acetate (100 mg, 0.367 mmol), Boc-Tyr (Bn) -OH (136 mg, 0.367 mmol), dimethylformamide (1 ml), diisopropylethylamine (0.25 ml, 1.468 mmol) and 2- (1H-benzotriazol-1-yl) -1,1,3,3-tetramethyluronium hexafluorophosphate (118 mg, 0.367 mmol) was kept at the temperature while stirring. room overnight. The mixture was diluted with ethyl acetate, washed twice with 1M hydrochloric acid, twice with aqueous sodium bicarbonate, once with brine, then dried (MgSO4) and concentrated. The residue was purified by flash chromatography (10% ethyl acetate / dichloromethane) to afford 162 mg (70%) of a colorless oil. The oil (160 mg, 0.255 mmol) was dissolved in dichloromethane (1 mL) and treated at 0 ° C with trifluoroacetic acid (1 mL). The resulting solution was allowed to warm to room temperature for 40 minutes, then evaporated to dryness at 30 ° C. The residue was dissolved in dichloromethane then re-evaporated to dryness. This procedure was repeated three times. The residue was dissolved in pyridine (0.5 mL) and treated with acetic anhydride (0.03 mL, 0.3 mmol) at 0 ° C. The resulting mixture was allowed to warm to room temperature and held at that temperature for 3.5 hours. It was then diluted with ethyl acetate, washed twice with 1M hydrochloric acid, twice with aq. Sodium bicarbonate solution, dried (MgSO4) and concentrated to give 128 mg (86%) of a colorless oil; IR (coating) 3290, 1751, 1649, 1602, 1568, 1513, 1455, 1438, 1375, 1224, 1179;¹H NMR (CDCl3) d 8.78 (1H, s), 8.33 (1H, d, J = 7.6), 7.33 (8H, m), 7.11 (4H, m), 6, 86 (2H, d, J = 8.5), 6.47 (1H, d, J = 7.6), 6.12 (1H, d, J = 7.6), 4.99 (2H, s ), 4.85 (1H, m), 4.69 (2H, s), 3.87 (2H, s), 3.62 (3H, s), 3.08 (2H, m), 1.96 (3H, s).

Methyl [6-benzyl-1,2-dihydro-2-oxo-3- (2-phenylethanesulfonyl) amino-1-pyridyl] -acetate (51 I). To a mixture of methyl [3-amino-6-benzyl-2-oxo-1,2-dihydro-1-pyridyl] acetate (49b) (1.0 g, 3.67 mmol), dichloromethane (15 ml) and triethylamine 3-phenylethanesulfonyl chloride (Zhong et al. J. Am. Chem. Soc. 113, pp. 2259-63 (1991)) was added with stirring. The mixture was allowed to stand overnight then poured into ethyl acetate. The resulting mixture was washed with aqueous sodium bicarbonate twice, three times with 1M hydrochloric acid, then with brine, then dried (MgSO4) and concentrated. The resulting pale brown solid was purified by flash chromatography (10% ethyl acetate / dichloromethane) to afford 1.25 g (77%) of a pale yellow solid: mp. 92-4 ° C; IR (KBr) 3181, 1737, 1646, 1595, 1565, 1454, 1241, 1220, 1150;

¹H NMR (CDCl3) d7.53 (1H, d, J = 7.5), 7.29 (10H, m), 6.10 (1H, d, J = 7.5), 4.75 (2H, s), 3.89 (2H, s), 3.67 (3H, s), 3.34 (2H, m), 3.14 (2H, m).

Methyl [6-benzyl-1,2-dihydro-2-oxo-3- (4-phenylbutyryl) amino-1-pyridyl] -acetate (51L) was isolated (74%) as colorless crystals: mp. 93-95 ° C; IR (KBr) 3285, 1747, 1683, 1642, 1591, 1563, 1512, 1455, 1220, 1181;¹H NMR (CDCl3) d 8.39 (1H, d, J = 7.6), 8.24 (1H, s), 7.2 (10H, m), 6.18 (1H, d, J = 7, 6), 4.7 (2H, s), 3.90 (2H, s), 3.67 (3H, s), 2.69 (2H, t), 2.40 (2H, t), 2, 04 (2H, m).

<img file="PL193391B1_D0070.tif" />

2 (S) 2- [1,2-Dihydro-2-oxo-3- (3-phenylpropionyl) amino) -1-pyridyl] propionic acid (52a).

To a solution of methyl 2 (S) 2- [1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] propionate (51a) (2.39 g, 7.3 mmol) in methanol (30 mL) 1M sodium hydroxide solution (15 mL, 15 mmol) was added at 0 ° C. The mixture was kept at this temperature for 2 hours, acidified with 1M hydrochloric acid (15.1 ml) and extracted with ethyl acetate. The extracts were washed with brine, dried (MgSO4), and concentrated to give 1.98 g (87%) of a colorless solid: [a] D²⁰ -75 ° (1, CH2Cl2); IR (KBr) 3301, 1724, 1693, 1637, 1563, 1523, 1453, 1233, 1216, 765;¹H NMR (CDCl 3) d 8.47 (2H, m), 7.20 (5H, m), 7.03 (1H, d), 6.36 (1H, t), 5.35 (1H, q) , 3.01 (2H, m), 2.70 (2H, m), 1.69 (3H, m).

The following compounds were prepared in a similar manner:

[6-Benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetic acid (52b) was isolated (100%) as a pale amber oil: IR (shell) 3291, 1738 , 1686, 1644,

PL 193 391 B1

1591, 1554, 1519, 1496, 1454, 1403, 1215, 1182; ¹H NMR (CDCl3) d 8.44 (1H, d, J = 7.8), 8.4 (1H, s), 7.21 (10H, m), 6.19 (1H, d, J = 7, 8), 4.71 (2H, s), 3.90 (2H, s), 2.99 (2H, m), 2.71 (2H, m).

[1,2-Dihydro-2-oxo-6-phenethyl-3- (3-phenylpropionyl) amino-1-pyridyl] acetic acid (52c) was isolated (92%) as a beige solid: mp. 214-6 °; IR (KBr) 3289, 1740, 1680, 1640;¹H NMR (d 6 -DMSO) d 9.24 (1H, s), 8.14 (1H, d, J = 7.7), 7.22 (10H, m), 6.11 (1H, d, J = 7.8), 4.78 (2H, s), 2.81 (8H, m).

[6-Butyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] acetic acid (52d) was isolated (99%) as a pale brown solid: mp. 132-4 ° C; IR (KBr) 3286, 1739, 1676, 1641, 1584, 1555, 1535, 1455, 1414, 1249, 1227, 1204;¹H NMR (CDCl3) d 8.42 (1H, d, J = 7.8), 8.37 (1H, s), 7.24 (5H, m), 6.19 (1H, d, J = 7 , 8), 4.82 (2H, s), 3.55 (1H, s), 3.00 (2H, t), 2.67 (2H, t), 2.53 (2H, t), 1 , 41 (4H, m), 0.94 (3H, t).

[1,2-Dihydro-6-methyl-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] acetic acid (52e) was isolated as a solid (100%): mp. 159-61 ° C; IR (KBr) 3335, 1731, 1686, 1642, 1536, 1516, 1430, 1420, 1401, 1222, 1195;¹H NMR (d 6 -DMSO) d 9.21 (1H, s), 8.13 (1H, d, J = 7.6), 7.20 (5H, m), 6.15 (1H, d, J = 7.6), 4.77 (2H, s), 2.87 (2H, m), 2.70 (2H, m), 2.25 (3H, s).

[1,2-Dihydro-2-oxo-6-phenyl-3- (3-phenylpropionyl) amino-1-pyridyl] acetic acid (52f) was isolated (100%) as a pale yellow foam: IR (KBr) 3271, 1747 , 1683, 1634, 1580, 1536, 1490, 1406, 1392, 1365, 1235, 1219; ¹H NMR (CDCl3) d 8.62 (1H, d, J = 7.7), 7.31 (10H, m), 6.48 (2H, s).

6.30 (1H, d, J = 7.7), 4.60 (2H, s), 3.03 (2H, m), 2.71 (2H, m).

[1,2-Dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] acetic acid (52g) was isolated (94%) as a colorless solid: mp. 195-7 ° C; IR (KBr) 3324, 1724, 1693, 1644, 1569, 1555, 1512, 1427, 1370, 1240;¹H NMR (d 6 -DMSO) d 9.31 (1H, s), 8.23 ​​(1H, d, J = 6.8),

7.36 (1H, dd, J = 6.8, 1.71), 7.25 (5H, m), 6.25 (1H, t), 4.66 (2H, s), 2.84 ( 4H, m).

2 (R, S) 2- [6-Benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -propionic acid (52h) was prepared by hydrolysis of 51h in aqueous tetrahydrofuran for 5 hours at 40 ° C. Yellow oil (95%) was obtained: IR (coating) 3330, 1734, 1686, 1643, 1600, 1587, 1553, 1524, 1498, 1208;¹H NMR (d 6 -DMSO) d 9.29 (1H, s), 8.18 (1H, d, J = 7.6), 7.21 (10H, m), 6.22 (1H, d, J = 7.6), 4.82 (1H, q, J = 6.6), 4.08 (2H, m), 2.76 (4H, m), 1.05 (3H, d, J = 6 , 6).

[3- (Acetyl-Tyr (Bn)) amino-6-benzyl-1,2-dihydro-2-oxo-1-pyridyl] acetic acid (52i) was isolated (93%) as a foam: IR (KBr) 3302 , 1731, 1646, 1603, 1562, 1512, 1454, 1428, 1379, 1231, 1178; ¹H NMR (CDCl3) d 9.48 (1H, s), 8.36 (1H, d, J = 7.6), 7.30 (8H, m), 7.10 (2H, m), 6, 85 (2H, d, J = 8.3), 6.91 (2H, d, J = 8.3), 6.71 (1H, d, J = 7.6), 4.95 (1H, m ), 4.90 (2H, s), 4.68 (2H, s), 3.92 (2H, s), 3.17-2.83 (2H, m), 1.92 (3H, s) .

[6-Benzyl-1,2-dihydro-2-oxo-3- (2-phenylethanesulfonyl) amino-1-pyridyl] acetic acid (52j) was isolated (100%) as a colorless solid: mp. 165-7 ° C; IR (KBr) 3174, 1760, 1646, 1593, 1567, 1497, 1453, 1424, 1326, 1225, 1140, 1127;¹H NMR (d 6 -DMSO) d 13.09 (1H, s), 9.08 (1H, s), 7.30 (11H, m), 6.02 (1H, d), 4.68 (2H, s) ), 4.99 (2H, s), 3.29 (2H, m), 3.03 (2H, m).

[6-Benzyl-1,2-dihydro-2-oxo-3- (2-phenylethoxy) carbonylamino-1-pyridyl] acetic acid (52k) was prepared (70%) by hydrolysis of 49i for 1 hour at 60 ° C ; IR (CH2Cl2) 1797, 1689, 1649, 1601, 1512, 734;¹H NMR (CDCl 3) d (8.39 (1H, s), 8.03 (1H, d), 7.81 (1H, s), 7.33-7.07 (10H, m), 6.13 (1H, d, J = 7.8), 4.72 (2H, s), 4.33 (2H, t, J = 7.0), 3.86 (2H, s), 2.93 (2H , t, J = 7.0).

[6-Benzyl-1,2-dihydro-2-oxo-3- (4-phenylbutyryl) amino-1-pyridyl] acetic acid (52L) was isolated (100%) as a white foam: mp. 159-161 ° C; IR (KBr) 3373-3310, 1787, 1726, 1691, 1649, 1599, 1567, 1517, 1367, 1215;¹H NMR (CDCl3) d 8.43 (1H, d, J = 7.7), 8.25 (1H, s), 7.37-7.09 (10H, m), 6.21 (1H, d, J = 7.7), 4.73 (2H, s), 4.15 (3H, s), 3.91 (2H, s), 2.67 (2H, t), 2.39 (2H, t ),

<img file="PL193391B1_D0071.tif" />

PL 193 391 B1

2 (S), N-3 (S) 2- [1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3 -yl) propionamide (53a). To a mixture of 2 (S) -2- [1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] propionic acid (52a) (1.1 g, 3.49 mmol), 3 (S), 2 (R, S) 3-allyloxycarbonylamino-2-benzyloxy-5-oxo-tetrahydrofuran (1.02, 3.49 mmol; Chapman, Biorg. Med. Chem. Lett., 2, p. 613-18 (1992)), bis (triphenylphosphine) palladium (II) chloride (55 mg), dichloromethane (35 ml) and dimethylformamide (1 ml) were added dropwise with stirring tri-n-butyltin hydride (1.7 ml, 6 3 mmol). The resulting mixture was stirred for 5 minutes then 1-hydroxybenzotriazole (946mg, 7mmol) was added. The mixture was cooled to 0 ° C and 1- (3-dimethylaminopropyl) -2-ethylcarbodiimide hydrochloride (740 mg, 3.84 mmol) was added. The mixture was allowed to stand overnight at room temperature then poured into ethyl acetate. The mixture was washed twice with 1M hydrochloric acid, twice with aqueous sodium bicarbonate, then with brine, dried (MgSO4) and concentrated. The residue was triturated with pentane. The resulting solid was purified by flash chromatography (40-60% ethyl acetate / hexane) to afford 1.28 g (73%) of a colorless solid: IR (KBr) 1796, 1692, 1647, 1595, 1557, 1512, 1119;¹H NMR (d6-DMSO) d 9.28, 9.26 (1H, 2xs), 8.77, 8.69 (1H, 2xd), 8.24, 8.20 (1H, 2xdd) , 7.20 (11H, m), 6.31, 6.26 (1H, 2xt), 5.65 (0.5H, d), 5.46 (0.5H, d), 5.41. 5.28 (1H, 2 xq), 4.7 (2.5H, m), 4.24 (0.5H, t), 3.24 (2H, m), 2.80 (4H, m), 1.51, 1.46 (3H, 2xd).

The following compounds were prepared in a similar manner:

N (3 (S)) 2 [6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3- yl) acetamide (53b) was formed as a foam (86%): IR (KBr) 3345, 3297, 1807, 1791, 1688, 1679, 1650, 1602, 1525, 1497, 1453, 1372, 1257, 1119; ¹H NMR (d6-DMSO) d 9.25 (0.5H, s), 9.23 (0.5H, s), 8.75 (0.5H, d, J = 6.5), 8.67 (0.5H, d, J = 7.4), 8.18 (1H, 2d), 7.21 (15H, m), 6.07 (1H, 2d), 5.65 (0.5H, d , J = 5.0), 5.38 (0.5H, s), 4.83-4.45 (4.5H, m), 4.19 (0.5H, m), 3.94.3 M. 83 (2H, m), 3.10-2.31 (6H, m).

N (3 (S)) 2 [1,2-dihydro-2-oxo-2-phenethyl-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-4- yl) acetamide (53c) was prepared (74%) as a mixture of anomers: ¹H NMR (d 6 -DMSO) d 9.71 (1H, d), 9.41 (0.5H, d), 9.25 (0.5H, d), 8.64 (1H, d, J = 7 , 7), 7.75 (15H, m), 6.61 (1H, 2d), 6.11 (0.5H, d), 5.93 (0.5H, s), 5.17 (5H, m), 4.77 (0.5H, m), 3.68-2.94 (2H, m), 3.32 (8H, m).

N (3 (S)) 2 [6-butyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3- yl) acetamide (53d) was prepared (74%) as a mixture of anomers: IR (KBr) 3300, 1791, 1689, 1645, 1597, 1566, 1546, 1514, 1454, 1417, 1378; ¹H NMR (CDCl3) d 8.38 (1H, d, J = 7.7), 8.13 (1H, s), 7.30 (10H, m), 6.18 (1H, t), 5, 47 (0.5H, d, J = 5.2), 5.43 (0.5H, s), 4.75 (4.5H, m), 4.38 (0.5H, m), 3. 08-2.35 (8H, m), 1.43 (4H, m), 0.95 (3H, t).

N (3 (S)) 2 [1,2-dihydro-6-methyl-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3- yl) acetamide (53e) was prepared (67%) as a mixture of anomers: IR (KBr) 3282, 1774, 1667, 1651, 1596, 1556, 1498, 1265, 1254, 1236, 1199, 1143; ¹H NMR (d6-DMSO) d 9.17 and 9.15 (1H, 2 x s), 8.89 (0.5H, d, J = 6.5), 8.73 (0.5H, d, J = 7.4), 7.25 (10H, m), 6.13 (1H, t), 5.64 (0.5H, d, J = 5.0), 5.45 (0.5H, s ), 4.89-4.61 (4.5H, m), 4.26 (0.5H, m), 3.17-2.36 (6H, m), 2.23 and 2.15 (3H , 2s).

N (3 (S)) 2- [1,2-dihydro-2-oxo-6-phenyl-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3 -yl) acetamide (53f) was prepared (73%) as a mixture of anomers: IR (KBr) 3296, 1792, 1691, 1643, 1595, 1514, 1489, 1453, 1420, 1373, 1230, 1118; ¹H NMR (d6-DMSO) d 9.40, 9.36 (1H, 2s), 8.70 (0.5H, d, J = 7.6), 8.52 (0.5H, d, J = 7.5), 8.29 (1H, dd), 7.25 (15H, m), 6.20 (1H, d, J = 7.6), 5.61 (0.5H, d, J = 5.0), 5.28 (0.5H, s), 4.78-4.20 (5H, m), 3.12-2.24 (6H, m).

N (3 (S)) 2- [1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3-yl) acetamide (53g) was prepared (70%) as a mixture of anomers: IR (KBr) 3336, 3290, 1791, 1691, 1646, 1595, 1582, 1556, 1518, 1454, 1376, 1351, 1150, 1122; ¹H NMR (d6-DMSO) d 9.26 (1H, 2s), 8.86 (0.5H, d, J = 6.4), 8.67 (0.5H, d, J = 7.5) , 8.23 ​​(1H, m), 7.40-7.13 (11H, m), 6.24 (1H, 2t, J = 7.2), 5.61 (0.5H, d, J = 5.0), 5.44 (0.5H, s), 4.83-4.59 (2.5H, m), 4.25 (0.5H, m), 3.15-2.34 ( 2H, m), 2.91-2.70 (4H, m). Analysis. Calculated for C27H27N3O6 H2O: C, 63.90; H, 5.76; N, 8.28. Found C, 63.70; H, 5.68; N, 8.22. MS FAB M⁺= 490 (M + 1).

2 (R, S), N (3 (S)) 2- [6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2- benzyloxy-5-oxotetrahydrofuran-3-yl) propionamide (53h) was prepared (89%) as a mixture of diastereomers. Data are given for a single diastereomer: IR (coating) 3356, 1788, 1677, 1645, 1602, 1517, 1455, 1377, 1203, 1167, 1120;¹H NMR (CDCl3) d 8.34 (1H, d, J = 7.6), 8.19 (1H, s),

PL 193 391 B1

7.38-7.13 (10H, m), 6.26 (1H, d, J = 7.6), 5.58 (1H, t), 5.31, 5.24 (1H, 2xs) , 4.62 (2H, 2q), 4.60 (1H, m), 4.27 (1H, m), 2.98, 2.68 (4H, 2m), 3.0-2.0 (2H , m), 1.42 (3H, d).

N (3 (S)) 2- [6-benzyl-1,2-dihydro-2-oxo-3- (N-acetyl-O-benzyltyrosinyl) amino-1-pyridyl] -N- (2-benzyloxy-5 -oxotetrahydrofuran-3-yl) acetamide (53i) was prepared (76%) as a mixture of anomers: IR (KBr) 1794, 1698, 1651, 1612, 1514, 1454, 1374, 1247, 1177, 1126; ¹H NMR (d6-DMSO) d 9.34, 9.31 (2 x 0.5H, 2s), 8.71 (1H, 2d), 8.38 (1H, m), 8.17 (1H, d ), 7.48-6.88 (19H, m), 6.08 (1H, 2d), 5.65 (0.5H, d, J = 5.0), 5.40 (0.5H, s ), 5.04 (2H, s), 4.68 (5.5H, m), 4.15 (0.5H, m), 3.95, 3.84 (2H, s + abq), 3. 20-2.40 (4H, m), 1.78 (3H, s).

N (3 (S)) 2- [6-benzyl-1,2-dihydro-2-oxo-3- (2-phenylethanesulfonyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3) -yl) acetamide (53j) was prepared (78%) as a mixture of anomers: IR (KBr) 3344, 1792, 1691, 1647, 1599, 1454, 1365, 1150, 1121, 973; ¹H NMR (d6-DMSO) d9.02.8.99 (1H, 2s), 8.80 (0.5H, d, J = 6.4), 8.70 (0.5H, d, J = 7 , 4), 7.26 (15H, m), 6.00 (1H, dd), 5.63 (0.5H, d, J = 5.0), 5.39 (0.5H, s), 4.68 (4.5H, m), 4.18 (0.5H, m), 3.90 (2H, m), 3.30-2.30 (6H, m).

N (3 (S)) 2- [6-benzyl-1,2-dihydro-2-oxo-3- (2-phenylethoxy) carbonylamino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3 -yl) acetamide (53k) was prepared (78%) as a mixture of anomers: IR (KBr) 3386, 1794, 1726, 1650, 1603, 1518, 1366, 1214, 699; ¹H NMR (CDCl3) d 8.03 (1H, bd), 7.63, 7.61 (1H, 2xs), 7.34-7.04 (15H, m), 6.21, 6.18 ( 1H, 2d), 5.44 (0.5H, d, J = 5.4),

5.37 (0.5H, s), 4.85, 4.83 (1H, 2d, J = 11.6, 11.5), 4.61-4.48, 4.32 (4H, 2m) , 4.4 (2H, t), 4.08, 4.03 (2H, 2bs), 3.07-2.78 (3H, m), 2.47-2.30 (1H, m).

N (3 (S)) 2- [6-benzyl-1,2-dihydro-2-oxo-3- (4-phenylbutyryl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran-3 -yl) acetamide (53L) was prepared (86%) as a colorless oil: IR (CH2Cl2) 1797, 1689, 1649, 1601, 1512, 734; ¹H NMR (CDCl3) d 8.42, 8.40 (1H, 2d, J = 7.6), 7.35-7.07 (15H, m), 6.21, 6.19 (1H, 2d, J = 7.6), 5.44 (0.5H, d), 5.37 (0.5H, s), 4.84, 4.81 (1H, 2d, J = 11.7, 11.4) , 4.73-4.48, 4.34 (4H, 2m), 4.05 (2H, m), 3.05-2.63, 2.46-2.30 (6H, 2m), 2, 01 (2H, m).

<img file="PL193391B1_D0072.tif" />

<td></td><td>R1</td><td>R2</td><td>R3</td>

<td>(and)</td><td>Ph (CH2) 2CO</td><td>H.</td><td>(S) Me</td>

<td>(b)</td><td>Ph (CH2) 2CO</td><td>CH2Ph</td><td>H.</td>

<td>(c)</td><td>Ph (CH2) 2CO</td><td>(CH2) 2Ph</td><td>H.</td>

<td>(d)</td><td>Ph (CH2) 2CO</td><td>nBu</td><td>H.</td>

<td>(e)</td><td>Ph (CH2) 2CO</td><td>Me</td><td>H.</td>

<td>(f)</td><td>Ph (CH2) 2CO</td><td>Ph</td><td>H.</td>

<td>(g)</td><td>Ph (CH2) 2CO</td><td>H.</td><td>H.</td>

<td>(h)</td><td>Ph (CH2) 2CO</td><td>CH2Ph</td><td>(R, S) -Me</td>

<td>(and)</td><td>AcTyr</td><td>CH2Ph</td><td>H.</td>

<td>(j)</td><td>Ph (CH2) 2SO2</td><td>CH2Ph</td><td>H.</td>

<td>(k)</td><td>Ph (CH2) 2OCO</td><td>CH2Ph</td><td>H.</td>

<td>(l)</td><td>Ph (CH2) 3CO</td><td>CH2Ph</td><td>H.</td>

<td>Acid</td><td colspan="3">3 (S), N (2 (S)) 3- (2- (1,2-dihydro-2-oxo-3- (3-phenylpropionylamino-1-pyridyl) -pro</td>

pioneylamino) -4-oxobutane (54a; F). Mixture 2 (S), N (3 (S)) 2- [1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl] -N- (2-benzyloxy-5-oxotetrahydrofuran -3-yl) propionamide 53a (1.28 g, 2.5 mmol), methanol (140 ml), ethyl acetate (60 ml) and 10% palladium on carbon (1.4 g) were stirred under a hydrogen atmosphere. After 2.5 hours, more catalyst (300 mg) was added and the hydrogenation continued for 1 hour. The mixture was filtered through 0.2 mM nylon membrane and concentrated. The residual oil was triturated with a mixture of methanol and ether to give 916 mg (87%) of colorless crystals: temp.

PL 193 391 B1 m.p. 198-200 ° C; [a] D²⁸ -120 ° (0.1, CH 3 OH); IR (KBr) 3330, 1794, 1688, 1644, 1583, 1556, 1515, 1427;¹H NMR (CD 3 OD) d 8.28 (1H, d), 7.35 (1H, d), 7.20 (5H, m), 6.36 (1H, t), 5.49 (1H, q) , 4.59 (1H, t), 4.25 (1H, m), 2.98, 2.74 (2x2H, 2xm), 2.59 (2H, m), 1.57 (3H, d). Analysis. Calculated for C21H23N3O6, 0.75H2O: C, 59.08; H, 5.78; N, 9.84. Found C, 59.24; H, 5.96; N, 9.84. FAB M⁺ = 414 (M + 1), 297, 165, 91.

The following compounds were prepared in a similar manner:

3 (S) 3- (6-Benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetylamino-4-oxobutanoic acid (54b; M) was isolated (59%) in the form of colorless crystals: mp. 115 ° C (decomposition); IR (KBr) 3440, 3297, 1718, 1646, 1598, 1565, 1526, 1496, 1260;¹H NMR (CD3OD) d 8.25 (1H, d, J = 7.7), 7.25 (10H, m), 6.15 (1H, 2d, each J = 7.7), 4.73 ( 2H, 2q), 4.59 (1H, m), 4.30 (1H, m), 3.95 (2H, s), 2.98 (2H, m), 2.75 (2H, m), 2.8-2.42 (2H, m). Analysis. Calculated for C27H27N3O6. 0.7H2O: C, 64.58; H, 5.70; N, 8.37. Found C, 64.51; H, 5.63; N, 8.38. MS FAB + M + = 490 (M + 1).

3 (S) 3- (1,2-Dihydro-2-oxo-6-phenethyl-3- (3-phenylpropionyl) amino-1-pyridyl) acetylamino-4-oxobutanoic acid (54c) was isolated (46%) as white solid: IR (KBr) 3375, 1694, 1643, 1586, 1561, 1515, 1377, 1254, 1188, 1070; ¹H NMR (CD3OD) d 8.18 (1H, d, J = 7.8), 7.22 (10H, m), 6.15 (1H, d, J = 7.8), 4.75 (2H , s), 4.58 (1H, m), 4.30 (1H, m), 3.01-2.28 (10H, m); MS FAB + M + = 504 (M + 1).

3 (S) 3- (6-Butyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetylamino-4-oxobutanoic acid (54d) was isolated (90%) as colorless crystals: mp. 120-5 ° C; IR (KBr) 3315, 1784, 1679, 1644, 1589, 1561, 1520, 1415, 1379, 1186;¹H NMR (CD3OD) d 8.22 (1H, d, J = 7.8), 7.24 (5H, m), 6.22 (1H, d, J = 7.8), 4.80 (2H , m), 4.60 (1H, s), 4.28 (1H, m), 2.98 (2H, m), 2.72 (2H, m), 2.58 (4H, m), 1 , 48 (4H, m), 0.97 (3H, t, J = 7.1). Analysis. Calculated for C24H29N3O6. 0.5H2O: C, 62.06; H, 6.51; N, 9.05. Found C, 62.08; H, 6.43; N, 9.01. MS FAB + M + = 456 (M + 1).

3 (S) 3- (1,2-Dihydro-6-methyl-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetylamino-4-oxobutanoic acid (54e) was isolated (85%) of a colorless substance constant: mp. 129-138 ° C; IR (KBr) 327, 3294, 1710, 1695, 1682, 1554, 1525, 1379, 1272, 1240;¹H NMR (CD3OD) d 8.19 (1H, d, J = 7.6), 7.19 (5H, m), 6.21 (1H, d, J = 7.6), 4.80 (2H , m), 4.59 (1H, m), 4.30 (1H, m), 2.98 (2H, m), 2.72 (2H, m), 2.80-2.40 (2H, m), 2.30 (3H, s). Analysis. Calculated for C21H22N3O6. H2O: C, 58.46; H, 5.84; N, 9.74. Found C, 58.82; H, 60.5, N, 9.42.

3 (S) 3- (1,2-Dihydro-2-oxo-6-phenyl-3- (3-phenylpropionyl) amino-1-pyridyl) acetylamino-4-oxobutanoic acid (54f), 73% as an off-white substance constant: mp. 140 ° C (decomposition). [a] D²⁴ - 8.5 ° (c 0.1, MeOH). IR (KBr) 3302, 1796, 1726, 1679, 1643, 1590, 1560, 1516, 1490, 1449, 1420, 1398, 1376, 1231;¹H NMR (CD3OD) d 8.36 (1H, d), 7.49-7.14 (10H, m), 6.27 (1H, dd), 4.54 (3H, m).

4.30 (1H, m), 3.0, 2.73 (2x2H, 2xm), 2.7-2.9 (2H, m).

3 (S) 3- (1,2-Dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetylamino-4-oxobutanoic acid (54g; G) was isolated (73%) as a foam: mp temp. 140-5 ° C (decomposition); IR (KBr) 3352, 3314, 1719, 1668, 1649, 1600, 1559, 1514, 1379, 1261;¹H NMR (CD3OD) d 8.32 (1H, d, J = 7.5), 7.19 (6H, m), 6.34 (1H, t), 5.1-4.6 (3H, m ), 4.32 (1H, m), 2.7 (6H, m). Analysis. Calculated for C20H21N3O6. 0.6H2O: C, 58.50; H, 5.45; N, 10.24. Found C, 58.43; H, 5.35; N, 9.85. MS FAB + M + = 400 (M + 1).

Acid 3 (S) N (2 (R, S)) 3- (2- (6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) propionylamino) - 4-Oxobutane (54h) was prepared (69%) as a colorless foam: mp. 120 ° C; [a] D²⁰ -16.0 ° (c, 0.11, CH2Cl2). IR (KBr) 3315, 1783, 1727, 1666, 1644, 1599, 1564, 1517, 1454, 1379;¹H NMR (CD 3 OD) d 8.23 ​​(1H, m), 7.27 (10H, m), 6.28 (1H, m), 4.84 (1H, m), 4.53 (1H, m) , 4.22 (1H, m), 4.10 (2H, m), 2.96 (2H, m), 2.72 (2H, m), 2.39 (2H, m), 1.21 ( 3H, m). Analysis. Calculated for C28H29N3O6. 1.25H2O: C, 63.93; H, 6.03; N, 7.99. Found C, 63.98; H, 5.85; N, 7.86. MS FAB (+) M + = 504 (M + 1).

3 (S) 3- (3- (2-Acetyl-L-tyrosinyl) amino-6-benzyl-1,2-dihydro-2-oxo-1-pyridyl) acetylamino-4-oxobutanoic acid (54i) was isolated (79 %) in the form of colorless crystals: mp. 193-6 ° C (decomp.); IR (KBr) 3284, 1644, 1565, 1519, 1455, 1429, 1407, 1375, 1267, 1251;¹H NMR (d6-DMSO / CDCl3) d 8.16 (1H, d, J = 7.7), 7.26 (5H, m), 7.03 (2H, d, J = 8.4), 6 . 61 (2H, d, J = 8.4), 6.03 (1H, d, J = 7.7), 4.58 (3H, m), 4.44 (1H, m), 4.13 (1H, m), 3.84 (2H, s), 3.07-2.30 (4H, m). Analysis. Calculated for C29H30N4O8. 2H2O: C, 58.19; H, 5.72; N, 9.36. Found C, 58.11; H, 5.63; N, 9.29. MS FAB + M + = 563 (M + 1).

3 (S) 3- (6-Benzyl-1,2-dihydro-2-oxo-3- (2-phenylethanesulfonyl) amino-1-pyridyl) acetylamino-4-oxobutanoic acid (54j) was isolated (85%) as colorless solid:

PL 193 391 B1 mp. 102-5 ° C; [a] D²³ -9.9 ° (c 0.1, MeOH); IR (KBr) 3452, 3328, 3155, 1719, 1679, 1645, 1594, 1567, 1453, 1425, 1357, 1307, 1225, 1148, 1132; ¹H NMR (CD3OD) d 7.52 (1H, d, J = 7.6), 7.33 (10H, m), 6.12 (1H, d, J = 7.6), 4.73 (2H , m), 4.58 (1H, d, J = 3.7), 4.34 (1H, m), 3.97 (2H, s), 3.29 (2H, m), 3.08 ( 2H, m), 2.75-2.37 (2H, m). Analysis. Calculated for C26H27N3O7S. 1.7H2O: C, 56.14; H, 5.51; N, 7.55. Found C, 55.20; H, 5.49; N, 7.29. MS FAB + M + = 526 (M + 1).

3 (S) 3- (6-Benzyl-1,2-dihydro-2-oxo-3- (2-phenylethoxy) carbonylamino-1-pyridyl) acetylamino-4-oxobutanoic acid (54k) was isolated (54%) as off-white solid: mp. 84-86 ° C; IR (KBr) 3373-3310, 1787, 1726, 1691, 1649, 1599, 1567, 1517, 1367, 1215;¹H NMR (CD3OD) d 7.93 (1H, bd, J = 7.4), 7.37-7.18 (10H, m), 6.15 (1H, d, J = 7.4), 4 , 77 (1H, d, J = 3.7), 4.67 and 4.58 (2H, 2m), 4.35 (2H, t, J = 6.9), 4.35 (1H, m) , 3.94 (2H, s), 2.98 (2H, t, J = 6.9), 2.76-2.39 (2H, m).

3 (S) 3- (6-Benzyl-1,2-dihydro-2-oxo-3- (4-phenylbutyryl) carbonylamino-1-pyridyl) acetylamino-4-oxobutanoic acid (54L) was isolated (50%) as white solid: mp. 89-93 ° C; IR (KBr) 3369-3302, 1678, 1645, 1594, 1565, 1517, 1379, 1258;¹H NMR (d4-methanol) d 8.25 (1H, d, J = 7.6), 7.37-7.18 (10H, m), 6.15 (1H, d, J = 7.4) , 4.74 (2H, m), 4.60 (1H, m), 4.30 (1H, m), 3.97 (2H, s), 2.76-2.37 (2H, m), 2.67 (2H, t), 2.45 (2H, t), 1.98 (2H, m). Analysis. Calculated for C28H29N3O6. 1.5H2O: C, 63.39; H, 6.08; N, 7.92. Found C, 63.69; H, 5.74; N, 7.83.

<img file="PL193391B1_D0073.tif" />

T-Butyl N-2 (3-benzyloxycarbonylamino-1,2-dihydo-2-oxo-1-pyridyl) acetyl-3-amino-5- (2,6-dichlorobenzoyloxy) -4-oxo-pentanoate (56a). Acetic acid (55a) (WO 93 21213) in THF (2 ml) was stirred at room temperature and 1-hydroxybenzotriazole (60 mg, 0.448 mmol) and dimethylaminopropyl-3-ethylcarbodiimide hydrochloride (47 mg, 0.246 mmol) were added. After 5 minutes, (2 drops) of water was added dropwise and stirring was continued for 20 minutes. Bis (triphenylphosphine) palladium (II) chloride (6 mg) was added followed by a solution of t-butyl 3- (allyloxycarbonylamino) -4-oxo-5- (2,6-dichlorobenzoyloxy) pentanoate (WO 93 16710) (103 mg, 0.224 mmol) in THF (1 ml). Tributyltin hydride (0.09 mL, 0.336 mmol) was added dropwise over 1 hour at room temperature. The mixture was stirred for an additional 3 hours and poured into ethyl acetate, washed with 1M HCl, aq. NaHCO3, brine, dried over MgSO4 and concentrated in vacuo. The residue was triturated with pentane and the supernatant was discarded. The residual solid was purified by flash chromatography (50% ethyl acetate / hexane) to give 92 mg (63%) of the title compound as a colorless oil: [a] D²⁶ -29.6 ° (c 1.1, CH2Cl2); IR (coating) 3377, 3365, 3332, 3312, 1733, 1691, 1650, 1599, 1515, 1366, 1261, 1153, 1068, 747;¹H NMR (CDCl3) d 8.09 (1H, d, J = 6.8), 7.84 (1H, s), 7.58 (1H, d, J = 8.3), 7.33 (8H , m), 7.02 (1H, dd, J = 6.9, 1.7), 6.33 (1H, t, J = 7.2), 5.20 (2H, s), 5.12 (2H, m), 4.89 (1H, dt), 4.65 (2H, m), 2.80 (2H, m),

1.38 (9H, s).

N-2- (6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetyl-3-amino-5- (2,6-dichlorobenzyloxy) -4- t-Butyl oxo-pentanoate (56b) was prepared as described for 56a to give the title compound (66%) as a colorless oil: IR (coating) 3364, 3313, 1738, 1688, 1648, 1600, 1566, 1514, 1433, 1369, 1254, 1152; ¹H NMR (CDCl3) d 8.40 (1H, d,

PL 193 391 B1

J = 7.6), 8.30 (1H, s), 7.28 (13H, m), 6.20 (1H, d, J = 7.6), 5.12 (2H, q), 4 , 86 (1H, m), 4.65 (2H, q), 4.06 (2H, s), 3.07-2.61 (6H, m), 1.39 (9H, s).

<img file="PL193391B1_D0074.tif" />

N-2 (3-Benzyloxycarbonylamino-1,2-dihydro-2-oxo-1-pyridyl) acetyl-3-amino-5- (2,6-dichlorobenzoyloxy) -4-oxo-pantanoic acid (57a; O). The ester of compound 56a (210 mg, 0.356 mmol) in dichloromethane (0.5 ml) was cooled to 0 ° C and treated with trifluoroacetic acid (0.5 ml), stirred and heated to 20 ° C for 30 minutes. The solution was evaporated to dryness under reduced pressure, redissolved in dichloromethane and concentrated (x 3). The residue was triturated with ethyl acetate and diluted with ether to give 162 mg (85%) of the title compound as a colorless solid: mp. 165-8 ° C (decomp.); [a] D²³ -38.8 ° (c 0.1 CH 3 OH); IR (KBr) 3332, 3275, 1723, 1658, 1649, 1581, 1562, 1526, 1432, 1385, 1258, 1218, 1206;¹H NMR (d 6 -DMSO) d 8.96 (1H, d, J = 7.3), 8.34 (1H, s), 7.85 (1H, dd, J = 7.3), 7.58 (3H, m), 7.35 (5H, m), 6.29 (1H, t, J = 7.3), 5.26 (2H, m), 5.15 (2H, s), 4. 69 (3H, m), 2.75 (2H, m). Analysis. Calculated for C27H23N3O9Cl2: C, 53.66; H, 3.84; N, 6.95. Found C, 53.36; H, 3.90; N, 6.81. MS (+ FAB); 604 (M + 1), 285, 241, 195, 173, 149, 91.

N-2- (6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetyl-3-amino-5- (2,6-dichlorobenzoyloxy) -4 acid -oxo-pentane (57b; P) was prepared as described for 57a to obtain the title compound (78%) as colorless crystals: temp.

top 116-120 ° C (decomp.); [a] D²⁶-41.1 ° (c 0.1, CH 3 OH): IR (KBr) 3299, 1739, 1715, 1689, 1666, 1645, 1598, 1563, 1518, 1432, 1209, 1151; ¹H NMR (d 6 -DMSO) d 9.24 (1H, s), 8.88 (1H, d, J = 7.6), 8.18 (1H, d, J = 7.7), 7.60 (3H, m), 7.26 (10H, m), 6.06 (1H, d, J = 7.7), 5.23 (2H, ABq), 4.69 (3H, m), 3. 93 (2H, s), 2.78 (6H, m). Analysis. Calculated for C35H31N3O8Cl2. H2O: C, 59.16; H, 4.68; N, 5.91. Found C, 59.38; H, 4.53; N, 5.84. MS (+ FAB); 694, (Cl = 35.37), (M + 1), 692 (Cl = 35.35), (M + 1).

PL 193 391 B1

<img file="PL193391B1_D0075.tif" />

T-Butyl (3S, 4R, S) N- (benzyloxycarbonyl) -3-amino-4- (2-benzoxazolyl) -4-hydroxybutanoate (59). To a solution of benzoxazole (250.2 mg, 2.1 mmol) in dry THF (10.5 ml) at -78 ° C under N2, 2.3M n-butyllithium solution in hexanes (0.96 ml, 2.2 mmoles). After stirring at -78 ° C for 20 minutes, dry solid MgBr2OEt2 (594.0 mg, 2.3 mmol) was added. The resulting heterogeneous mixture was warmed to -45 ° C and stirred for 15 minutes. The reaction mixture was then re-cooled to -78 ° C and aldehyde 58 solution (Graybill et al., Int. J. Peptide Protein Res., 44, pp. 173-82 (1993)) (644.6 mg, 2.1) was added dropwise. mmol) in THF (10.5 mL). The mixture was stirred at -78 ° C for 30 minutes, warmed to 0 ° C for 1 hour, and then stirred at room temperature for 16 hours. The reaction was quenched with 5% sodium bicarbonate (2.0 mL) and the THF was removed in vacuo. The resulting aqueous residue was extracted four times with methylene chloride. The combined extracts were washed with brine, dried (MgSO4), filtered, and concentrated in vacuo to give 880.0 mg of the crude product. Flash chromatography (45:55 ethyl acetate / hexane) provided 567.2 mg (63%) of the title compound as an oil and a mixture of diastereomers at C-4. IR (coating) 3324, 2976, 1726, 1517, 1455, 1368, 1243, 1159, 1048, 747;¹H NMR (CDCl3) d 7.71-7.64 (1H, m), 7.52-7.48 (1H, m), 7.37-7.20 (7H, m), 5.91 (1H , brd, J = 9.0), 5.79 (1H, d, J = 9.0), 5.41-4.78 (4H, m), 4.75-4.54 (1H, m) , 2.91-2.51 (2H, m), 1.42 (9H, s), 1.37 (9H, s).

T-Butyl (3S, 4R, S) 3-amino-4- (2-benzoxazolyl) -4-hydroxybutanoate (60). A solution of ester 59 (189.0 mg, 0.44 mmol) in ethanol (5.0 mL) was treated with 10% palladium on carbon (20.5 mg) and stirred under an atmosphere of H 2 O for 21 hours. The mixture was filtered through Celite® and the solvent was evaporated to afford 125.0 mg (98%) of the crude amine (60) as an oil. The product was used without further purification.¹H NMR (CDCl3) d 7.73-7.64 (1H, m), 7.51-7.42 (1H, m), 7.35-7.22 (2H, m), 6.48 (3H , brs), 5.58 (1H, d, J = 3.0), 5.27 (1H, d, J = 6.5), 4.23-4.05 (1H, m), 2.92 -2.63 (2H, m), 1.36 (9H, s), 1.33 (9H, s).

T-Butyl (3S, 4R, S) N- (N-benzyloxycarbonyl- (S) -valinyl- (S) -alaninyl) -3-amino-4- (2-benzoxazolyl) -4-hydroxybutanoate (61). A solution of amine 60 (261.4 mg, 0.89 mmol), Z-Val-Ala-OH (286.9 mg, 0.89 mmol) (prepared by standard peptide synthesis methods), and hydroxybenzotriazole (120.3 mg, 0.05 89 mmol) in DMF (3.0 mL) at 0 &amp; lt; 0 &amp; gt; C was treated with hydrochloride 66

1-ethyl-3- [3- (dimethylamino) propyl] carbodiimide (179.2 mg, 0.93 mmol). The mixture was warmed to room temperature and stirred for 16 hours. Diluted with ethyl acetate and washed twice with 1M sodium bisulfate solution, twice with saturated sodium bicarbonate solution, then with water and brine. The organic layer was dried (MgSO4), filtered, and concentrated in vacuo to yield 494.8 mg of the crude product. Flash chromatography (methylene chloride / methanol 95: 5) provided 480.9 mg (91%) of the title compound as a yellow solid: mp. 81-83 ° C; IR (KBr) 3312, 2974, 1723, 1709, 1529, 1455, 1368, 1243, 1156, 747;¹H NMR (CDCl3) d 7.79 (0.5H, d, J = 8.0), 7.73-7.20 (9.5H, m), 6.15 (1H, t, J = 8, 5), 5.74 (0.5H, brd, J = 5.5), 5.45 (1H, brd, J = 7.5), 5.28-5.20 (0.5H, m), 4.82-4.11 (3.5H, m), 4.78-4.55 (1H, m), 4.40-4.22 (1H, m), 2.95-2.51 (2H , m), 2.12-1.95 (1H, m), 1.45-1.32 (12H, m), 1.11-0.81 (6H, m), ¹³C NMR (CDCl3) d 173.14, 172.94, 171.82, 171.03, 170.78, 165.98, 165.45, 157.29, 157.17, 151.23, 151.10, 140.92, 140.82, 136.83, 136.79, 128.91, 128.52, 125.75, 124.97, 120.60, 120.40, 111.38, 81.82, 81, 68, 70.27, 68.97, 67.44, 60.43, 50.74, 50.55, 49.18, 49.07, 36.87, 36.57, 32.37, 28.51, 19.88, 19.80, 18.53. Analysis. Calculated for C31H40N4O8. H2O: C, 60.57; H, 6.89; N, 9.11. Found C, 60.84; H, 6.64; N, 9.09. MS (+ FAB); 597 (M + 1); 541, 91.

T-Butyl (3S) N- (N-benzyloxycarbonyl- (S) -valinyl- (S) -alaninyl) -3-amino-4- (2-benzoxazolyl) -4-oxobutanoate (62). Alcohol 61 (100.3 mg, 0.17 mmol) was dissolved in methylene chloride (2.0 ml) and the Dess-Martin reagent (142.6 mg, 0.34 mmol) was added (Ireland et al. J. Org. Chem. ., 58, p. 2899 (1993); Dess et al. J. Org. Chem., 48, pp. 4155-4156 (1983)). The resulting mixture was stirred for 22 minutes, then partitioned between saturated sodium thiosulfate solution, saturated sodium bicarbonate solution (1: 1, 10 ml) and ethyl acetate (10 ml). The resulting organic phase was washed with saturated sodium thiosulfate solution, saturated sodium bicarbonate solution (1: 1), saturated sodium bicarbonate solution and brine. The organic phase was dried (MgSO4), filtered and concentrated in vacuo to yield 111.3 mg of the crude product. Flash chromatography (methylene chloride / methanol 95: 5) provided 97.3 mg (96%) of the title compound as an oil: [a] D²³ -11.74 ° (c 0.95, CH2Cl2); IR (CH2Cl2) 3419, 2974, 1721, 1677, 1501, 1369, 1221, 1156;¹H NMR (CDCl3) d 7.89-7.74 (1H, m), 7.73-7.22 (10H, m), 5.89 (1H, d, J = 9.0) 5.72 ( 1H, m), 5.10 (2H, q, J = 12.5), 4.73 (2H, m), 4.20 (1H, dd, J = 7.0, 8.5), 3, 30 (1H, dd, J = 5.0, 16.5), 3.03 (1H, dd, J = 5.5, 16.5), 2.18-1.97 (1H, m), 1 , 39 (3H, d, J = 7.0), 1.34 (9H, s), 0.94 (3H, d, J = 6.0), 0.90 (3H, d, J = 6, 0), ¹³C NMR (CDCl3) d 186.46, 172.73, 171.90, 170.13, 157.17, 156.28, 151.16, 140.99, 136.99, 129.39, 129.08, 128.66, 128.59, 126.49, 123.06, 112.55, 82.73, 67.60, 60.84, 53.75, 49.41, 38.58, 32.05, 28, 52, 19.85, 19.32, 18.51. MS (+ FAB); 595 (M + 1); 539, 91.

(3S) N- (N-benzyloxycarbonyl- (S) -valinyl- (S) -alaninyl) -3-amino-4- (2-benzoxazolyl) -4-oxobutanoate (63.0). A solution of ester 62 (95.0 mg, 0.16 mmol) in a 1: 1 mixture of methylene chloride and trifluoroacetic acid (10.0 ml) was stirred for 1 hour under a dry atmosphere of N₂. The solution was concentrated in vacuo, taken up in ether and concentrated again. This was repeated six times to give the crude product as an off-white solid. Flash chromatography (methylene chloride / methanol 95: 5) provided 60.0 mg (69%) of the title compound as a white solid. The product was in the form of a mixture of three isomers in CD3OD, consisting of a ketone (one isomer, c 44%), its acyloxyketal (two isomers at C-4, c 56%): mp. 156-159 ° C; [a] D²⁶ -45.6 ° (c 0.13, methanol); IR (KBr) 3440, 2967, 1713, 1703, 1638, 1531, 1427;¹H NMR (CD3OD) d 7.93-7.24 (9H, m), 5.59 (1H, brt), 5.16-5.00 (2H, m), 5.0-4.78 (1H , m), 4.50-4.22 (1H, m), 3.95-3.81 (1H, m), 3.11 (2H, d, J = 6.5), 3.05-2 , 92 (1H, m), 2.70-2.39 (1H, m), 2.08-1.89 (1H, m), 1.19-0.78 (9H, m). Analysis. Calculated for C27H30N4O8. 0.5H2O: C, 59.22; H, 5.71; N, 10.23. Found C, 59.48; H, 5.36; N, 10.17. MS (+ FAB); 539 (M + 1), 91.

PL 193 391 B1

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7-methoxybenzoxazole (65a). A mixture of 2-nitro-6-methoxyphenol (2.62 g, 15.5 mmol) (EP 333176) and 10% palladium on carbon (130 mg) in ethanol (50.0 ml) was stirred under an atmosphere of H2 for 75 minutes. The mixture was filtered through Celite® and treated immediately with p-toluenesulfonic acid (32.0 mg) and triethyl orthoformate (6.45 mL, 38.8 mmol) then heated to reflux under N2. After 20 hours, p-toluenesulfonic acid (30.0 mg) and triethyl orthoformate (6.45 ml, 38.8 mmol) were added. After heating a total of 44 hours, the mixture was allowed to cool and concentrated in vacuo. The resulting residue was purified by flash chromatography (ethyl acetate / hexane 25:75) to afford 1.97 g (85%) of the title compound as a yellow solid: mp. 28-31 ° C; IR (coating) 1629, 1497, 1434, 1285, 1097;¹H NMR (CDCl3) d 8.09 (1H, s), 7.40 (1H, d, J = 8.0), 7.28 (1H, t, J = 8.0), 6.89 (1H, d, J = 8.0), 4.02 (3H, s); ¹³C NMR (CDCl3) d 152.84, 145.82, 142.50, 139.99, 125.75, 113.42, 108.80, 56.97. Analysis. Calculated for C8H7N1O2. 0.1H2O: C, 63.65; H, 4.81; N, 9.29. Found C, 63.43; H, 4.88, N, 9.05. MS (+ FAB); 150 (M + 1).

4-methoxybenzoxazole (65b). To a suspension of 4-hydroxybenzoxazole (2.00 g, 14.8 mmol) (Musser et al., J. Med. Chem., 30, pp. 62-67 (1987)) in acetone (80.0 ml) was added dried K.₂WHAT₃(2.25 g, 16.3 mmol) followed by iodomethane (1.38 mL, 22.2 mmol). The mixture was refluxed under N2 for 4.5 hours, then filtered and concentrated.

In vacuo to give the crude product. The resulting residue was purified by flash chromatography (ethyl acetate / hexane 25:75) to yield 2.0 g (91%) of the title compound as a crystalline solid: mp. 72-84 ° C; IR (KBr) 3089, 1619, 1610, 1503, 1496, 1322, 1275, 1090, 1071, 780, 741;¹H NMR (CDCl3) d 8.02 (1H, s), 7.32 (1H, t, J = 8.0), 7.18 (1H, d, J = 8.0), 6.81 (1H , d, J = 8.0), 4.04 (3H, s). Analysis. Calculated for C8H7NO2: C, 64.42; H, 4.73; N, 9.39. Found C, 64.40; H, 4.84; N, 9.31; m / z (EI) 149 (M⁺+ 1, 100%).

T-Butyl (3S, 4R, S) N- (allyloxycarbonyl) -3-amino-4-hydroxy-4- (2- (7-methoxybenzoxazolyl)) butanoate (66a). To a stirred solution of 7-methoxybenzoxazole 65a (548.6 mg, 3.68 mmol) in anhydrous THF (18.5 ml) at -78 ° C under N2 was added dropwise 1.56 M of n-butyllithium in hexanes (2. 47 ml, 3.86 mmol) and a yellow colored solution was formed. After stirring at -78 ° C for 20 minutes, dry solid MgBr2OEt2 (1.045 g, 4.05 mmol) was added.

The resulting heterogeneous mixture was warmed to -45 ° C and stirred for 15 minutes. The reaction mixture was then re-cooled to -78 ° C and a solution of (S) -Alloc-Asp (t-Bu) H was added dropwise.^1b (946.4 mg, 3.68 mmol) in THF (18.5 mL). The mixture was stirred at -78 ° C for 30 minutes, warmed to 0 ° C, and stirred for 1 hour. The resulting homogeneous mixture was warmed to room temperature and stirred for 16 hours. The reaction was quenched with 5% sodium bicarbonate solution (3.5 mL) and the THF was removed in vacuo. The resulting aqueous residue was extracted with methylene chloride (6 x). The combined extracts were washed with brine, dried (MgSO4), filtered and concentrated in vacuo to give 1.8 g of the crude product. Flash chromatography (ethyl acetate / hexane 40:60) provided 1.21 g (81%) of the title compound, an oil, as a mixture of diastereomers at C-4; IR (CH2Cl2) 3425, 2983, 1725, 1504, 1290, 1157, 1101;¹H NMR (CDCl3) d 7.35-7.19 (2H, m), 6.89-6.81 (1H, m), 6.00-5.57 (2H, m), 5.32-5 . 05 (3H, m), 4.68-4.35 (3H, m), 4.01 (3H, s), 2.86-2.59 (2H, m), 1.45 (9H, s ). 1.41 (9H, s); ¹³C NMR (CDCl3) d 171.18, 171.09, 165.80, 165.30, 156.71, 156.60, 145.65, 142.76, 142.71, 140.82, 140.72, 133.23, 125.72, 118.41, 118.21, 113.07, 112.87, 108.95, 82.16.70.28, 69.98, 66.52, 66.39, 57, 03, 52.57, 52.29, 37.83, 36.86, 28.65. Analysis. Calculated for C20H26N2O7. 0.6H2O: C, 57.57; H, 6.57; N, 6.72. Found C, 57.49; H, 6.34, N, 6.60. MS (+ FAB); 407 (M + 1); 351, 307, 154.

T-butyl (3S, 4R, S) N- (allyloxycarbonyl) -3-amino-4-hydroxy-4- (2- (4-methoxybenzoxazolyl) butanoate (66b) was prepared according to the method described for compound 66a. 29 g (26%, 68% based on recovered starting material) of the title compound as an oil mixture of diastereomers at C-4: IR (CH2Cl2) 3400,1725, 1625, 1505, 1369, 1354, 1281, 1263, 1226, 1158 , 1092, 1048; ¹H NMR (CDCl3) d 7.34-7.24 (1H, m), 7.16 (1H, d, J = 8.2), 6.79 (1H, d, J = 7.9), 6 .00-5.50 (2H, m), 5.30-5.05 (3H, m), 4.70-4.35 (4H, m), 4.02 (3H, s), 2.90 -2.45 (2H, m), 1.45-1.41 (9H, 2xs). Analysis. Calculated for C20H26N2O7. 0.4H2O: C, 58.07; H, 6.53; N, 6.77. Found C, 58.09; H, 6.41; N, 6.63. MS (+ FAB); 407 (M + 1.88%); 351 (100).

(3S, 4R, S) N- (N-acetyl- (S) - (O-tert-butyl-tyrosinyl) - (S) -valinyl- (S) -alaninyl) -3-amino-4-hydroxy-4 - (t-Butyl 2- (7-methoxybenzoxazolyl) butanoate (67a) For a solution of benzoxazole 66a (481.9 mg, 1.19 mmol) and Ac-Tyr (^volBu) -Val-Ala-OH (585.3 mg, 1.30 mmol) in methylene chloride (3.5 ml) and DMF (3.5 ml) were added with stirring bis (triphenylphosphine) palladium (II) chloride (18 0 mg) then tributyltin hydride (0.80 mL, 2.96 mmol) was added dropwise. Hydroxybenzotriazole (320.4 mg, 2.37 mmol) was added and the mixture was cooled to 0 ° C. Ethyl-3- [3- (dimethylamino) propyl] carbodiimide hydrochloride (278.2 mg, 1.42 mmol) was added and the mixture was allowed to warm to room temperature and stirred for 16.5 hours. The reaction mixture was diluted with ethyl acetate and washed twice with 1M sodium hydrogen sulfate solution, twice with saturated sodium bicarbonate solution, water and brine. The organic layer was dried (MgSO4), filtered, and concentrated to give 2.0 g of the crude product. Flash chromatography (methylene chloride / methanol 95: 5) provided 844.0 mg (94%) of the title compound as a white solid: mp. 205 ° C; IR (KBr) 3399, 3304, 2977, 1729, 1643, 1506, 1367, 1290, 1161;¹H NMR (d6-DMSO) d 8.24-7.78 (4H, m), 7.43-7.32 (2H, m), 7.23 (2H, d, J = 8.5), 7 , 16-7.07 (1H, m), 6.93 (2H, d, J = 8.5), 6.52, 6.40 (1H, 2 xd, J = 5.5, J = 5, 0), 5.03, 4.78-4.49, 4.45-4.16 (5H, brt, 2xm), 4.05, 4.04 (3H, 2xs), 3.08-2 , 35 (14H, m), 2.11-1.89 (1H, m), 1.83 (3H, s), 1.49-1.32, 1.15, 1.0-0.81 ( 27H x, xm, J = 7.0); ¹³C NMR (d6-DMSO) d 175.55, 175.18, 173.88, 173.75, 173.05, 169.23, 157.28, 148.55, 146.16, 143.21, 136, 63, 133.55, 128.87, 127.17, 115.78, 111.92, 84.02, 81.50, 71.40, 61.15, 60.05, 57.79, 53.39, 51.62, 43.76, 40.52, 34.58, 32.52, 31.60, 26.35, 23.11, 22.71, 21.76. Analysis. Calculated for C39H55N5O10. 0.5H2O: C, 61.40; H, 7.40; N, 9.18. Found: C, 61.43; H7.31; N, 9.07. MS (+ FAB); 754 (M⁺ + 1); 698, 338, 267.

PL 193 391 B1 (3S, 4R, S) N- (N-acetyl- (S) - (O-tert-butyl-tyrosinyl) - (S) -valinyl- (S) -alaninyl) -3-amino-4 -hydroxy-4- (2- (4-methoxybenzoxazolyl)) t-butylbutanoate (67b) was prepared according to the method described for compound 67a to give 1.05 g (94%) of the title compound as a fine white powder: m.p. . 210-213 ° C (decomposition); IR (KBr) 3284, 2977, 1736, 1691, 1632, 1536, 1505, 1452, 1392, 1367, 1258, 1236, 1161, 1091;¹HNMR (d6-DMSO) d 8.20-7.75 (4H, m), 7.40-7.10 (4H, m), 7.00-6.80 (3H, m), 6.45, 6.34 (1H, 2xd, J = 5.3, J = 5.0), 5.00-4.10 (5H, m), 4.00, 3.99 (3H, 2xs), 3 . 00-2.25 (4H, m), 1.95 (1H, m), 1.79 (3H, s), 1.39-0.80 (27H, m). Analysis. Calculated for C39H55N5O10. 0.5H2O: C, 61.40; H, 7.40; N, 9.18. Found C, 61.58; H, 7.38; N, 8.91. MS (+ FAB); 754 (M⁺ + 1,30%); 72 (100).

(3S) N- (N-acetyl- (S) -O-tert-butyl-tyrosinyl) - (S) -valinyl- (S) -alaninyl) -3-amino-4- (2- (7-methoxybenzoxazolyl) T-Butyl-4-oxobutanoate (68a). Dess-Martin reagent (1.082 g, 2.55 mmol) (Ireland et al., J. Org. Chem., 58, p. 2899 (1993); Dess et al. J. Org. Chem., 48, pp. 4155-4156 (1983). The resulting mixture was stirred for 1 hour and then partitioned between saturated sodium thiosulfate solution, saturated sodium bicarbonate solution (1: 1, 86.0 mL) and ethyl acetate (86.0 mL). The resulting organic phase was washed successively with a saturated sodium thiosulfate solution, a saturated sodium bicarbonate solution (1: 1), a saturated sodium bicarbonate solution and brine. The organic phase was dried (MgSO4), filtered, and concentrated in vacuo to yield 660.0 mg of crude product. Flash chromatography (methylene chloride / methanol, 94: 6) provided 636.0 mg (100%) of the title compound as a white solid: mp. 209C; [a] D²⁴-21.8 ° (c 0.16, methanol); IR (KBr) 3395, 3294, 2977, 1722, 1641, 1535, 1505, 1161;¹H NMR (CDCl3) d 8.43-8.16 (1H, m), 7.97-7.62 (2H, m), 7.49-7.14 (3H, m), 7.08-6 , 95 (3H, m), 6.89-6.73 (2H, m), 5.81-5.68 (1H, m), 5.16-4.86 (2H, m), 4.53 (1H, brt), 4.03 (3H, s), 3.16-2.84 (4H, m), 2.11-1.84 (4H, m), 1.46-1.14 (21H , m), 0.78 (6H, m); ¹³C NMR (CDCl3) d 186.28, 173.39, 171.90, 171.19, 171.03, 169.89, 156.43, 154.75, 146.32, 142.88, 140.98, 132, 31, 130.54, 126.98, 124.73, 114.95, 111.42, 82.44, 78.71, 58.92, 57.20, 54.91, 53.47, 48.77, 39.43, 38.15, 32.79, 29.44, 28.60, 23.55, 20.27, 19.70, 19.34. MS (+ FAB); 752 (M⁺ + 1); 696, 336, 265.

(3S) N- (N-acetyl- (S) - (O) -tert-butyl-tyrosinyl) - (S) -valinyl- (S) -alaninyl) -3-amino-4- (2- (4- t-Butyl 4-oxobutanoate (68b) methobenzoxazolyl)) was prepared according to the method described for ketone 68a to give 420 mg (55%) of the title compound as a white solid: mp. 211-213 ° C (decomp.); [a] D²⁴ - 23.9 ° (c 0.82, methanol); IR (KBr) 3277, 3075, 1723, 1690, 1632, 1530, 1506, 1392, 1269, 1234, 1160, 1094;¹H NMR (CDCl3) d 8.15 (1H, brs), 7.7 (2H, brs), 7.46 (1H, t, J = 8.3), 7.24 (2H, d, J = 8 , 3), 7.10 (1H, brs), 7.03 (2H, d, J = 8.3), 6.83 (3H, m), 5.74 (1H, q, J = 6.9 ), 5.00 (2H, m), 4.51 (1H, t, J = 7.0), 4.07 (3H, s), 3.20-2.95 (4H, m), 2. 00 (4H, m), 1.42 (3H, d, J = 6.8), 1.35 (9H, s), 1.23 (9H, s), 0.86 (6H, d, J = 6.7). MS (+ FAB); 752 (M⁺ +1,7%); 72 (100).

(3S) N- (N-acetyl- (S) -tyrosinyl- (S) -valinyl- (S) -alaninyl) -3-amino-4- (2- (7-methoxybenzoxazolyl)) - 4-oxobutanoate (69a ; R). A solution of ester 68a (600.0 mg, 0.80 mmol) in a 1: 1 mixture of methylene chloride and trifluoroacetic acid (65.0 ml) was stirred for 1 hour under a dry atmosphere of N₂. The solution was concentrated in vacuo, taken up in ether and concentrated again. This was repeated six times to give the crude product as an off-white solid. Flash chromatography (95: 5 to 80:20 gradient of methylene chloride and methanol) provided 420.8 mg (83%) of the title compound as a hygroscopic white solid. The product appeared as a mixture of three isomers in CD3OD, consisting of a ketone (c 50%), its acyl ketone (two isomers at C-4, c 50%): temp. top decomposes above 150 ° C; [a] D²⁴ - 33.2 ° (c 0.17, methanol); IR (KBr) 3300, 1715, 1658, 1650, 1531, 1517, 1204;¹H NMR (CD3OD) d 7.46-7.19 (2H, m), 7.16-6.91 (3H, m), 6.70-6.59 (2H, m), 5.62-5 . 49 (1H, m), 5.00-4.72 (1H, dim), 4.69-4.51 (1H, m), 4.49-4.08 (2H, m), 4.05 -3.89 (3H, m), 3.16-2.47 (4H, m), 2.05-1.78 (4H, m), 1.41-1.11, 1.05-0, 70 (9H, 2xm). Analysis. Calculated for C31H37N5O10. 3H2O: C, 53.67; H, 6.25; N, 10.10. Found: 53.76; H, 5.56; N, 10.28. MS (+ FAB); 640 (M⁺ + 1); 435, 147.

(3S) N- (N-acetyl- (S) -tyrosinyl- (S) -valinyl- (S) -alaninyl) -3-amino-4- (2- (4-methoxybenzoxazolyl)) -4-oxobutanoate t- butyl (69b; S), prepared according to the method described for the acid 69a to give the hygroscopic title compound, 252 mg (96%). The product was in the form of a mixture of three isomers in CD3OD consisting of a ketone and its acyloxyketal (two isomers at C-4). The product appeared as a single isomer in d-6 DMSO: mp. 200-203 ° C (decomposition); [a] D²⁴ - 38.0 ° (c 0.23, methanol); IR (KBr) 3289, 2968, 1718, 1713, 1658, 1634, 1548, 1517, 1506, 1461, 1453, 1393, 1713, 1658, 1634, 1548, 1517, 1506, 1461, 1453, 1393, 1369, 1268, 1228, 1174,

PL 193 391 B1

1092; ¹HNMR (d6-DMSO) d 9.20 (1H, brs), 8.71 (1H, d, J = 6.2), 8.10 (2H, m), 7.83 (1H, d, J = 8.7), 7.61 (1H, t, J = 8.2), 7.46 (1H, d, J = 8.2), 7.08 (3H, m), 6.65 (2H, d, J = 8.3), 5.50 (1H, q, J = 6.5), 4.50 (1H, m), 4.37 (1H, m), 4.20 (1H, m) , 4.05 (3H, s), 3.09-2.77 (4H, m), 1.94 (1H, m), 1.79 (3H, s), 1.23 (3H, d, J = 7.0), 0.82 (6H, m). Analysis. Calculated for C31H37N5O10. 1.5H2O: C, 55.85; H, 6.05; N, 10.51. Found C, 55.21 H, 5.69; N, 10.13. MS (+ FAB); 640 (M⁺+ 1,2%); 107 (100).

<img file="PL193391B1_D0077.tif" />

T-Butyl (3S) N- (allyloxycarbonyl) -3-amino-4-oxo-5- (1,2-dioxo-phenylethyloxy) -pentanoate (80). To a solution of t-butyl (3S) N- (allyloxycarbonyl) -3-amino-5-bromo-4-oxo-pentanoate (WO 93 16719) (2.17 g, 6.20 mmol) in dimethylformamide (30 ml) was added potassium fluoride (792 mg, 13.6 mmol) with stirring followed by benzoylformic acid (1.02 g, 6.82 mmol). After stirring for 140 minutes, the mixture was quenched with water (50 ml) and extracted with ethyl acetate (2 x 50 ml). The combined organic extracts were washed with water (4 x 50 mL) followed by brine (50 mL), dried (MgSO4), and concentrated. An oil was obtained which was purified by flash chromatography (20-45% ethyl acetate in hexane) to give 2.44 g (94%) of a colorless oil: [a] D²⁰ -35.0 ° (c 1.41, CH2Cl2); IR (coating) 3359, 2981, 2938, 1752, 1740, 1726, 1712, 1512, 1369,

PL 193 391 B1

1285, 1177, 1053, 991, 939, 688; ¹H NMR (CDCl3) d 8.15 (2H, m), 7.66 (1H, m), 7.53 (2H, m), 5.90 (2H, m), 5.33 (2H, m) , 5.31 (1H, d, J = 16.9), 5.18 (1H, d, J = 16.9), 4.63 (3H, m), 3.03 (1H, dd, J = 17.3, 4.6), 2.74 (1H, dd, J = 17.3, 4.9), 1.44 (9H, s). MS (CI) 420 (M.⁺ + 1,20%); 364 (100).

T-Butyl (3S) N- (allyloxycarbonyl) -3-amino-5-hydroxy-4-oxo-pentanoate (81). A mixture of ester 80 (2.40 g, 5.71 mmol), tetrahydrofuran (200 ml) and 1M aqueous potassium bicarbonate (200 ml) was stirred vigorously at room temperature for 18 hours. The layers were separated and the aqueous portion was extracted with ethyl acetate (100 ml). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), and concentrated. The residue was purified by flash chromatography (ethyl acetate in hexane) to give 1.48 g of a pale yellow oil: [a] D²⁰ -5.9 ° (c 1.06, CH2Cl2); IR (coating) 3345, 2981, 2936, 1739, 1725, 1712, 1692, 1515, 1368, 1259, 1158, 1051; ¹H NMR (CDCl3) d 5.92 (2H, m), 5.30 (2H, m), 4.36-4.69 (5H, m), 3.05 (1H, dd, J = 17.4 , 4.3), 2.93 (IH, t), 2.70 (IH, dd, J = 17.4, 4.9), 1.43 (9H, s). Analysis. Calculated for C19H21N1O6: 0.25H2O: C, 53.51; H, 7.43; N, 4.80. Found C, 53.61; H, 7.18; N, 4.71. MS (CI) 280 (M.⁺+ 1,87%); 232 (100).

T-Butyl (3S) N- (allyloxycarbonyl) -3-amino-5- (2,6-dichlorophenyl-methoxy) -4-oxo-pentanoate (82). A mixture of alcohol 81 (1.44 g, 5.01 mmol), 2,6-dichlorobenzyl iodide (Abraham et al., J. Chem. Soc. Pp. 1605-1607 (1936)) (4.31 g, 15, 0 mmol), silver oxide (2.32 g, 10.0 mmol) and dichloromethane (25 ml) was heated to reflux for 45 hours with stirring. The mixture was allowed to cool to room temperature then diluted with water (50 ml) and then extracted with ethyl acetate (50 ml, 25 ml). The organic layer was washed with water (50 mL) then brine (50 mL), dried (MgSO4), and concentrated. The residue was purified by flash chromatography (10-100% ethyl acetate in hexane) to afford 1.65 g (74%) of a colorless oil : [a] D²⁰+ 8.8 ° (c 1.13, CH2Cl2); IR (coating) 3339, 2980, 2935, 1724, 1712, 1503, 1438, 1368, 1246, 1156, 1106, 770;¹H NMR (CDCl3) d 7.33 (2H, m), 7.22 (1H, dd), 5.92 (2H, m), 5.28 (2H, m), 4.87 (2H, m) , 4.67 (1H, m), 4.58 (2H, br d), 4.56 (1H, d, J = 16.9), 4.31 (1H, d, J = 16.9), 3.04 (1H, dd, J = 16.9, 4.5),

2.77 (1H, dd, J = 16.7, 4.9), 1.40 (9H, s). Analysis. Calculated for C20H25Cl2N1O6. O / 25H2O: C, 53.28; H, 5.70; N, 3.11. Found C, 53.15 H, 5.52; N, 2.98. MS (CI); 446 (M⁺, 27%); 390 (100).

T-Butyl (3R, S) N- [N-phenylmethyloxycarbonylvalaninylalaninyl] -3-amino-5- (2,6-dichlorophenylmethyloxy) -4-oxo-pentanoate (83a). 1- (3-Dimethylamino-propyl) -3-ethylcarbodiimide hydrochloride (379 g, 1 98 mmol) and 1-hydroxybenzotriazole (486 mg, 3.60 mmol). The mixture was stirred for 15 minutes, then ether 82 (802 mg, 1.80 mmol) and bis (triphenylphosphine) palladium (II) chloride (ca. 5 mg) were added. Tributyltin hydride (785mg, 725L, 2.70mmol) was then added dropwise over 20 minutes and the resulting solution was stirred for 3.75h before quenching with 1M hydrochloric acid (50ml). The mixture was extracted twice with ethyl acetate. The combined organic extracts were washed with 1M hydrochloric acid, twice with saturated aqueous sodium bicarbonate, water then brine, dried (MgSO4) and concentrated. The residue was purified by flash chromatography (10-30% ethyl acetate-dichloromethane) to afford 941 mg (79%) of a pale yellow solid: mp. 148-52 °; IR (KBr) 3287, 3070, 1730, 1691, 1641, 1536, 1369, 1289, 1247, 1156;¹H NMR (CDCl3) d 7.33 (8H, m), 7.23 (1H, dd), 6.61 (1H, br, d). 5.42 (1H, br, d), 5.11 (2H, s), 4.85 (3H, m), 4.50 (1H, m), 4.40 (1H, d, J = 16, 9), 4.26 (1H, d, J = 16.9), 4.02 (1H, m), 2.99 (1H, dd, J = 16.8, 4.7), 2.73 ( 1H, dd, J = 16.8, 5.0), 2.09 (1H, m), 1.37 (12H, m), 0.96 (3H, d, J = 6.9), 0, 91 (3H, d, J = 6.8). Analysis. Calculated for C32H41C12N3O8. 0.25H2O: C, 57.25; H, 6.23; Cl, 10.57; N, 6.26. Found C, 57.18; H, 6.23; Cl 10.58; N, 5.95. MS (+ FAB); 667 (M⁺ + 1, 1%); 666 (3), 159 (25), 91 (100).

T-Butyl (3R, S) N - [(N-acetyl-Ot-butyl tyrosinyl) -valaninyl-alaninyl] -3-amino-5- (2,6-dichlorophenylmethyloxy) -4-oxo-pentanoate (83b) was prepared by the method described for compound 83a to give 554 mg (64%) of a colorless solid: mp. 184-6 °; IR (KBr) 3282, 3075, 1736, 1690, 1633, 1536, 1508, 1366, 1236, 1161;¹H NMR (d 6 -DMSO) d 8.49 (1H, d), 8.14 (1H, d), 8.08 (1H, d), 7.84 (1H, d), 7.43 (3H, m), 7.14 (2H, d), 6.83 (2H, d), 4.71 (2H, s), 4.51 (2H, m), 4.36 (2H, dd), 4. 17 (2H, m), 2.93 (1H, m), 1.94 (1H, m), 1.74 (3H, s), 1.37 (9H, s), 1.23 (12H, m ). 0.83 (6H, m). MS (+ FAB); 793 (M⁺ + 1.4%); 737 (5), 681 (1), 178 (40), 159 (45), 136 (100), 107 (40). MS (- FAB); 792 (20), 791 (40) 447 (100).

(R, S) N- [N- (Phenylmethyloxy) carbonyl-valinyl-alaninyl] -3-amino-5- (2,6-dichlorophenylmethyloxy) -4-oxo-pentanoic acid (84a, Y). To a stirred solution of ester 83a (918 mg, 1.38 mmol) in dichloromethane (20 ml) was added trifluoroacetic acid (5 ml). The mixture was 72

After drying for 2.5 hours, it was evaporated to dryness. The residue was treated with ether (25 ml) and evaporated to dryness. This operation was repeated three times. The resulting product was triturated with ether (10 mL) then dried to give 730 mg (87%) of a light brown powder: mp. 156-60 ° C; IR (KBr) 3282, 2965, 1702, 1694, 1642, 1536, 1438, 1246, 1230;¹H NMR (d 6 -DMSO) d 8.48 (1H, d), 8.09 (1H, d), 7.47 (9H, m), 5.02 (2H, s), 4.70 (2H, s), 4.49 (1H, m), 4.37 (2H, dd), 4.27 (1H, m), 3.88 (1H, m), 2.75 (1H, dd), 2, 54 (1H, dd), 1.96 (1H, m), 1.19 (3H, s), 0.84 (6H, m). Analysis. Calculated for C28H33Cl2N3O8. 0.5H2O: C, 54.27; H, 5.53; Cl, 11.45; N, 6.78; Found C, 54.49; H, 5.39; Cl, 11.33; N, 6.73. MS (+ FAB); 610 (M⁺+ 1, 10%); 91 (100).

(R, S) N- [N- (acetyl) tyrosinyl-valinyl-alaninyl] -3-amino-5- (2,6-dichlorophenylmethyloxy) -4-oxo-pentanoic acid (84b; W) was obtained as a colorless powder (95%) as used for compound 84a. Temp. top 165-8 °; IR (KBr) 3295, 2968, 1733, 1642, 1517, 1438, 1231, 1105;¹H NMR (d 6 -DMSO) d 9.1 (1H, br s), 8.48 (1H, br, d), 8.14 (1H, br, d), 8.02 (1H, br, d), 7.81 (1H, br, d), 7.45 (3H, m), 7.02 (2H, d), 6.62 (2H, d), 4.70 (2H, s), 4.12 -4.53 (3H, m), 3.60 (3H, m), 2.51-2.92 (4H, m), 1.96 (1H, m), 1.75 (3H, s), 1.21 (3H, d), 0.83 (6H, m). Analysis. Calculated for C31H38Cl2N4O9. H2O: C, 53.22; H, 5.76; Cl, 10.14; N, 8.09; Found C, 53.33; H, 5.54; Cl, 10.02; N, 7.85. MS (+ FAB); 682 (M⁺2, 30%); 681 (67), 158 (100).

Example 6

Inhibition constants (Ki) and IC50 values ​​were obtained for some compounds of the invention using UV-visible substrate enzyme assays, fluorescent substrate assays, and cell assays as described in Example 2. For compounds 22e, 54b, 54j, 54k, 57b, 85, 86, 87, 88, 89, 90, 91, 92, 88, 102a-c, 106a-c, 108a-c, 114a, 114b, 115, 121, 125a, 125b, 126, 127, 128, 129, 130, 131, 132a, 132b, 133, 135a, 136, 137, 138, 139, 140, 141, 142, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 157, 158, 159, 160, 161, 162 and 163, the following Ki, and IC50 values ​​were determined using the above-indicated assays. Compound formulas 22e, 54b, 54j, 54k and 57b are shown in Example 5. The formulas of other compounds are provided in Example 7.

<td></td><td colspan="2">Test</td>

<td>Relationship</td><td>UV-visible</td><td>Cells</td>

<td></td><td>Ki (mm)</td><td>I50 (mm)</td>

<td> 1</td><td> 2</td><td> 3</td>

<td>22e</td><td> 0,19</td><td> &gt;20</td>

<td>54b</td><td></td><td> 20</td>

<td>54j</td><td></td><td> 10</td>

<td>54k</td><td></td><td> 6,6</td>

<td>57b</td><td></td><td> 2,2</td>

<td> 85</td><td> 0,0035</td><td> 9,8</td>

<td> 86</td><td> 0,175</td><td> 4,0</td>

<td> 87</td><td> 7,2</td><td> 35,0</td>

<td> 88</td><td> 0,9</td><td></td>

<td> 89</td><td> 0,018</td><td></td>

<td> 90</td><td> 0,42</td><td> 6,2</td>

<td> 91</td><td> 0,26</td><td> &gt;25</td>

<td> 92</td><td> 3,8</td><td></td>

<td> 98</td><td> 0,535</td><td> 4,0</td>

<td>102a</td><td></td><td> 4,0</td>

To be continued

<td> 1</td><td> 2</td><td> 3</td>

<td>102b</td><td> 0,29</td><td> 1,75</td>

<td>102c</td><td> 0,68</td><td></td>

<td>106a</td><td> 2,3</td><td> 30,0</td>

<td>106b</td><td> 0,2</td><td> 2,9</td>

<td>106c</td><td> 3,8</td><td> &gt;30,0</td>

<td>108a</td><td></td><td> 17,5</td>

<td>108b</td><td> 0,4</td><td> 25,0</td>

<td>108c</td><td> 0,43</td><td></td>

<td>114a</td><td> 0,12</td><td> 3,8</td>

<td>114b</td><td> 3,7</td><td></td>

<td> 115</td><td> 0,345</td><td> 6,0</td>

<td> 121</td><td> 4,3</td><td></td>

<td>125a</td><td> 0,39</td><td> &gt;30,0</td>

<td>125b</td><td> 0,060</td><td> 0,30</td>

<td> 126</td><td> 0,45</td><td> 1,5</td>

<td> 127</td><td> 0,39</td><td> 8,0</td>

<td> 128</td><td> 0,04</td><td> 7,5</td>

<td> 129</td><td> 0,59</td><td> 25,0</td>

<td> 130</td><td></td><td> 1,20</td>

<td> 131</td><td> 12,0</td><td> 30,0</td>

<td>132a</td><td> 5,0</td><td> &gt;30,0</td>

<td>132b</td><td> 12,5</td><td></td>

<td> 133</td><td> 50,0</td><td> &gt;30,0</td>

<td>135a</td><td> 0,090</td><td> 0,90</td>

<td>135b</td><td> 0,32</td><td> 0,95</td>

<td> 136</td><td></td><td> 1,0</td>

<td> 137</td><td> 0,04</td><td> 0,25</td>

<td> 138</td><td></td><td> 0,375</td>

<td> 139</td><td> 0,350</td><td> 2,0</td>

<td> 140</td><td> 0,87</td><td> &gt;30,0</td>

<td> 141</td><td> 0,670</td><td></td>

<td> 142</td><td></td><td> 1,75</td>

<td> 144</td><td> 0,32</td><td> &gt;20,0</td>

<td> 145</td><td> 0,34</td><td> 8,5</td>

<td> 146</td><td> 0,16</td><td> 3,8</td>

<td> 147</td><td> 0,26</td><td> 8,5</td>

<td> 148</td><td> 6,3</td><td> 30,0</td>

To be continued

<td> 1</td><td> 2</td><td> 3</td>

<td> 149</td><td> 14,0</td><td> &gt;30,0</td>

<td> 150</td><td> 10,0</td><td> 30,0</td>

<td> 151</td><td> 13,0</td><td> 30,0</td>

<td> 152</td><td> 8,8</td><td></td>

<td> 153</td><td> 0,24</td><td></td>

<td> 154</td><td> 0,042</td><td> 2,4</td>

<td> 155</td><td> 0,023</td><td></td>

<td> 156</td><td> 0,001</td><td> 2,7</td>

<td> 157</td><td> 0,26</td><td></td>

<td> 158</td><td> 1,1</td><td></td>

<td> 159</td><td> 0,0017</td><td> 8,0</td>

<td> 160</td><td> 0,145</td><td> 2,25</td>

<td> 161</td><td> 0,011</td><td></td>

<td> 162</td><td> 0,0025</td><td></td>

<td> 163</td><td> 0,0028</td><td> 1,2</td>

Example 7

The following compounds were prepared in a manner similar to that used in the synthesis of 69a: 126, 127, 128, 129, 135a, 135b, 137, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155 , 156, 157, 159, 160, 162 and 163.

<img file="PL193391B1_D0078.tif" />

PL 193 391 B1

<img file="PL193391B1_D0079.tif" />

<img file="PL193391B1_D0080.tif" />

PL 193 391 B1

<img file="PL193391B1_D0081.tif" />

PL 193 391 B1

<img file="PL193391B1_D0082.tif" />

PL 193 391 B1

Compound 158 was synthesized in a similar manner to that used in the synthesis of compound (K).

<img file="PL193391B1_D0083.tif" />

Compound 130 was synthesized in a similar manner to that used in the synthesis of compound 56b.

<img file="PL193391B1_D0084.tif" />

Compounds 131, 136, 138 and 142 were synthesized in a similar manner to that used in the synthesis of 57b.

<img file="PL193391B1_D0085.tif" />

PL 193 391 B1

Compounds 132, 132b, 139, 140 and 141 were synthesized in a similar manner to that used in the synthesis of 47a. The starting material for compound 140 was prepared as described in Robl, et al.

J. Am. Chem. Soc., 116, pp. 2348-2355 (1994). The starting compound for compound 141 was prepared as described in Wyvratt, et al. Pept. Struct. Funct. Proc. (8th Am. Pept. Symp.), (1983) or US Patent 4,415,496.

<img file="PL193391B1_D0086.tif" />

Compound 133 was synthesized in a similar manner to that used in the synthesis of compound 47b.

<img file="PL193391B1_D0087.tif" />

PL 193 391 B1

Compound 161 was synthesized in a similar manner to that used in the synthesis of compound 125a.

<img file="PL193391B1_D0088.tif" />

Compounds 22e, 54b, 54j, 54k and 57b were synthesized as described in Example 5.

Compounds 85, 86, 87, 88, 89, 90, 91, 92, 98, 102a, 102b, 102c, 106a, 106b, 106c, 108a, 108b,

108c, 114a , 114b, 115, 121, 125a and 125b were synthesized as follows.

<img file="PL193391B1_D0089.tif" />

N- (N-acetyl-tyrosinyl-valinyl- (4 (R) -allyloxyprolinyl)) -3 (S) -amino-4-oxobutanoic acid (85).

Stage. A. N-tert-butoxycarbonyl-4 (R) -allyloxyproline. N-tert-butoxycarbonyl (4R) -hydroxyproline (9.25 g, 40 mmol) was added to a solution of 60% sodium hydride (3.36 g, 84 mmol) in 100 ml of anhydrous tetrahydrofuran and stirred for 2 hours at room temperature. Allyl bromide (6.9 ml, 80 mmol) was added to the mixture and the mixture was heated to reflux for 6 hours. The reaction was quenched with ice flakes, more water was added, and the mixture was washed with hexane. The aqueous layer was acidified with 10% sodium bisulfate solution and extracted with ethyl acetate (2 x 150 ml). The combined extracts were dried over anhydrous sodium sulfate, filtered and evaporated to yield 5 g of the title compound without further purification.¹H NMR (CDCl3; exists as rotamers) d 5.92-5.82 (1H, m), 5.3-5.14 (2H, m), 4.5-4.31 (1H, m), 4.16-4.05 (1H, m), 4.04-3.9 (1H, m), 3.79-3.5 (3H, m), 2.43-2.2 (1.5H , m), 2.15-2.10 (0.5H, m), 1.45 (4.5H, s), 1.35 (4.5H, s).

Step B. 4 (R) -allyloxyproline methyl ester hydrochloride. N-tert-butoxycarbonyl-4 (R) -allyloxyproline (5 g, 18.4 mmol) was refluxed for 6 hours in 50 ml of saturated methanolic hydrogen chloride solution. The mixture was evaporated in vacuo to give 3.78 g of the title compound as a yellow gum;¹H NMR (CDCl3) d 5.83-5.72 (1H, m), 5.24-5.14 (1H, d), 5.13-5.08 (1H, d), 4.55-4 , 3 (3H, m), 4.25-4.15 (1H, m), 3.9 (1.5H, s), 3.78 (1.5H, s), 3.7-3.28 (3H, m), 2.45-2.32 (IH, m), 2.2-2.05 (IH, m).

Step C. N-Acetyl-tyrosinyl-valinyl- (4 (R) -allyloxyproline) methyl ester. 4 (R) -allyloxyproline methyl ester hydrochloride (1.05 g, 4.75 mmol) and N-acetyl-Tyr-Val-OH (1.68 g, 5.21 mmol) was dissolved in 10 mL of 1: 1 dichloromethane and dimethylformamide and cooled to 0 ° C. To the cooled mixture was added diisopropylethylamine (1mL, 5.93mmol) followed by N-hydroxybenzotriazole (0.769g, 5.69mmol) and 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (1.18g, 6.2 mmoles). After stirring for 2 hours, the mixture was warmed to room temperature and stirred for 16 hours. The mixture was poured into 150 ml of ethyl acetate and washed each time with 50 ml of water, 10% sodium hydrogen sulfate solution and 10% sodium hydrogen carbonate solution. The organic layer was dried over sodium sulfate, filtered and evaporated to give a light yellow solid. This material was purified by flash chromatography eluting with dichloromethane / methanol / pyridine (100: 3: 0.5) to give 780 mg of the title compound.¹H NMR (CD3OD) d 7.02-6.96 (2H, d), 6.67-6.63 (2H, d), 5.95-5.85 (1H, m), 5.34-5 , 27 (IH, d), 5.16PL 193 391 B1

-5.13 (1H, d), 4.53-4.38 (3H, m), 4.28-4.22 (1H, m), 3.82-3.73 (1H, m), 3 , 72 (3H, s), 3.04-2.88 (2H, m), 2.85-2.72 (2H, m), 2.45-2.34 (1H, m), 2.08 -1.95 (2H, m), 1.92 (3H, s), 1.00-0.92 (6H, 2xd).

Step D. N- (N-Acetyl-tyrosinyl-valinyl- (4 (R) -allyloxyprolinyl)) -3 (S) -amino-4-oxobutanoic acid tert-butyl ester semicarbazone. N-acetyl-tyrosinyl-valinyl- (4-allyloxyproline) methyl ester (770 mg, 1.57 mmol) was dissolved in 20 mL of tetrahydrofuran and 4 mL of methanol. Lithium hydroxide (145 mg, 3.46 mmol) was added to the mixture and stirred at room temperature. After two hours, 10% hydrogen chloride solution was added to the mixture and the mixture was evaporated in vacuo to give a solid residue and then partitioned between 5 mL of water and 50 mL of ethyl acetate. The organic layer was separated and evaporated in vacuo to give 430 mg of the acid which was used directly in the next step.

From N-acetyl-tyrosinyl-valinyl-4-allyloxyproline (420 mg, 0.88 mmol) and 3-amino-4-oxobutyric acid tert-butyl ester semicarbazone (184 mg, 0.8 mol, Graybill et al., Int J. Protein Res., 44, pp. 173-82 (1994)) afforded 100 mg (20%) of the title compound as a white amorphous solid: ¹H NMR (CD3OD) d 7.24-7.2 (1H, m), 7.04-6.97 (2H, d), 6.73-6.65 (2H, d), 5.98-5 , 86 (1H, m), 5.35-5.24 (1H, d), 5.17-5.12 (1H, m), 4.12-3.98 (2H, m), 3.72 -3.72 (1H, m), 2.98-2.92 (3H, m), 2.38-2.32 (1H, m), 2.1-2.02 (2H, m), 1 . 92 (3H, s), 0.98-0.89 (6H, 2xd)

Step E. N- (N-Acetyl-tyrosinyl-valinyl- (4 (R) -allyloxyprolinyl)) -3 (S) -amino-4-oxobutanoic acid (85). The protecting group was removed from N- (N-acetyl-tyrosinyl-valinyl- (4 (R) -allyloxyprolinyl)) -3 (S) -amino-4-oxobutanoic acid tert-butyl ester (100 mg)₁ Example 3, compound K, step C was obtained to give 44.2 mg (53%) of the title compound: ¹H NMR (CD3OD) d 7.04-6.97 (2H, d), 6.72-6.65 (2H, d), 5.97-5.86 (1H, m), 5.32-5 . 25 (1H, d), 5.17-5.12 (1H, d), 4.62-4.40 (3H, m), 4.30-4.13 (2H, m), 4.12 -3.96 (3H, m), 3.75-3.68 (1H, m), 2.99-2.92 (1H, m), 2.78-2.70 (1H, m), 2 , 70-2.48 (2H, m), 2.35-2.30 (1H, m), 2.17-1.95 (2H, m), 1.92 (3H, s), 0.98 -0.88 (6H, 2xd).

Compounds 86 and 87 were prepared by a method similar to that described for the synthesis of 69a in Example 5:

<img file="PL193391B1_D0090.tif" />

N-Acetyl- (S) -valinyl- (4- (S) -phenoxy) prolinyl-3 (S) -amino-4- (7-methoxybenzoxazol-2-yl) -4-oxo-butanoic acid (86). N-acetyl- (S) -valinyl- (S) - (4- (S) -phenoxy) proline was converted to compound 86 to give a white powder:¹H NMR (DMSO-d6) d 8.75 (d, 1H), 7.6-7.2 (m, 4H), 7.0-6.8 (m, 4H), 5.5 (m, 1H) ), 5.05 (s, 1H), 4.5 (t, 1H), 4.29 (t, 1H), 4.0 (s, 3H), 4.0-3.8 (m, 2H) , 3.0-2.8 (dd, 2H), 2.3 (m, 1H), 2.09 (m, 1H), 1.95-1.8 (m, 2H), 1.78 (s , 3H), 1-0.7 (dd, 6H).

<img file="PL193391B1_D0091.tif" />

PL 193 391 B1

N-acetyl (4- (R) -phenoxy) prolinyl-3 (S) -amino-4- (7-methoxybenzoxazol-2-yl) -4-oxo-butanoic acid (87): N-acetyl- (S) - (4- (S) -phenoxy) proline was converted to compound 87 to give a white powder: ¹H NMR (DMSO-d6) d 9.1 (d, 1H), 8.76 (d, 1H), 7.6-7.2 (m, 4H), 7.0-6.9 (m, 4H ), 5.55 (m, 1H), 5.45 (m, 1H), 5.0 (m, 2H), 4.56 (t, 1H), 4.40 (t, 1H), 4.0 (s, 3H), 3.9 (dd, 1H), 3.76 (d, 1H), 3.64 (d, 1H), 3.1-2.9 (m, 1H), 2.8 ( m, 1H), 2.50 (m, 1H), 2.3-2.2 (m, 1H), 2.09 (m, 1H), 1.95 and 1.75 (2xs, 3H, rotamers ).

<img file="PL193391B1_D0092.tif" />

N-2- (6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetyl-3 (S) -amino-5-hydroxy-4-oxo acid pentane (88). N-2- (6-Benzyl-1,2-dihydro-2-oxo-3- (3-phenyl-propionyl) amino-1-pyridyl) acetyl-3 (S) -amino-5-hydroxy- acid tert-butyl ester 4-Oxo-pentane was prepared from 52b and 81 according to the method described for the synthesis of 83a to give a white solid (45%):¹H NMR (CDCl3) d 8.40 (d, 1H), 8.20 (s, 1H), 7.4-7.1 (m, 1H), 6.18 (s, 1H), 4.72 ( m, 1H), 4.65-4.5 (q, 2H), 4.4-4.2 (dd, 2H), 4.0 (s, 2H), 3.04 (t, 2H), 2 , 9 (dd, 1H), 2.76 (t, 2H), 2.55 (dd, 1H), 1.39 (s, 9H).

The resulting product was converted to compound 89 as described in Example 5 for compound 84a to afford the title compound (42%) as a white solid: ¹H NMR (CDCl3) d 8.5 (d, 1H), 8.1 (d, 1H), 8.0 (m, 1H), 7.4-7.1 (m, 11H), 6.3 ( d, 1H), 4.9-4.8 (m, 2H), 4.6-4.4 (m, 2H), 4.3 (dd, 1H), 4.1 (s, 2H), 3 , 3 (t, 1H), 3.05 (t, 2H), 2.8-2.6 (m, 3H).

Compounds 89 and 90 were prepared by a similar method as described in Example 5 for the preparation of 84a.

<img file="PL193391B1_D0093.tif" />

N-Acetyl- (S) -tyrosinyl- (S) -valinyl- (S) -alaninyl-3 (S) -amino-5- (2-chlorobenzyloxy) -4-oxo-pentanoic acid (89) was prepared from Ac- Tyr-Val-Ala-OH and (3S) N-allyloxycarbonyl) -3-amino-5- (chlorophenylmethoxyl) -4-oxo-pentanoate (prepared in a similar manner to compound 82) to give a white solid: ¹H NMR (DMSO-d6) d 9.15 (s, 1H), 8.5 (d, 1H), 7.98 (d, 1H), 7.75 (d, 1H), 7.55-7, 3 (m, 4H), 7.0 (d, 1H), 6.6 (d, 2H), 4.6-4.3 (m, 6H), 4.3-4.1 (m, 2H) , 2.9 (d, 1H), 2.76 (dd, 1H), 2.7-2.5 (m, 2H), 1.95 (m, 1H), 1.75 (s, 3H), 1.2 (d, 3H), 0.9-0.7 (dd, 6H).

<img file="PL193391B1_D0094.tif" />

PL 193 391 B1

N-2- (6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetyl-3-amino-5- (2-chlorobenzyloxy) -4-oxo acid -pentane (90) was prepared from 52b and t-butyl N- (allyloxycarbonyl) -3-amino-5- (2-chlorophenylmethoxyl) -4-oxo-pentanoate (prepared in a similar manner to compound 82) to give a white solid: ¹H NMR (DMSO-d6) d 9.2 (s, 1H), 8.75 (d, 1H), 7.7-7.1 (m, 14H), 6.4 (d, 1H), 4. 65 (d, 6H), 4.56 (s, 1H), 4.6-4.35 (dd, 1H), 3.9 (s, 2H), 2.9-2.6 (m, 6H) .

<img file="PL193391B1_D0095.tif" />

N-2- (6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionyl) amino-1-pyridyl) acetyl-3 (S) -amino-5- (5- (2, 6-Dichlorophenyl) thiazol-2-yl) -4-oxo-pentanoic acid (91) was prepared from 52b and 3- (allyloxy) -amino-4 - [(2,6-dichloro-phenyl) -thiazole acid tert-butyl ester -2-yl] -4-hydroxybutyric (99) as described for the preparation of compound 69a to give an off-white powder: ¹H NMR (DMSO-d6) d 9.32 (s, 1H), 9.05 (d, 1H), 8.27 (d, 1H), 8.18 (d, 1H), 7.7 (d, 1H), 7.6 (t, 1H), 7.4-7.1 (m, 11H), 6.1 (d, 1H),

5.64 (m, 1H), 4.8-4.6 (dd, 2H), 3.85 (s, 2H), 3.02 (m, 1H), 2.9-2.7 (m, 4H).

<img file="PL193391B1_D0096.tif" />

3- (S) - (2- (3 [3- (S) - (4-Hydroxy-phenyl) -propionylamino] -2-oxo-azepan-1-yl) -acetylamino) -4-oxo-butyric acid ( 92) was prepared from 2- (3 [3- (S) - (4-hydroxy-phenyl) -propionylamino] -2-oxo-azepan-1-yl) -acetic acid and N-allyloxycarbonyl-4-amino-5- benzyloxy-2-oxotetrahydrofuran (Chapman, Biorg. Med. Chem. Lett., 2, pp. 613-18 (1992)) in a manner similar to that described for the synthesis of 54a to afford the title compound as a white solid: ¹H NMR (DMSO-d6) d9.10-9.20 (s, 1H), 8.40 (s, 1H), 7.88 (d, 1H), 7.0 (d, 2H), 6.64 (d, 2H), 4.60 (t, 1H), 4.10 (q, 2H), 3.94.2 (m, 2H), 3.6 (m, 1H), 3.18 (d, 2H), 2.70 (t, 2H), 2.40 (m, 2H), 1.85-1.40 (m, 8H).

<img file="PL193391B1_D0097.tif" />

PL 193 391 B1

4-ethoxymethylene-2-styryl-4H-oxazol-5-one (94) was prepared according to Cornforth, The Chemistry of Penicillin, Clarke, Johnson, Robinson (eds), Princeton University Press, p. 804 (1949).

4-Oxo-3- (3-phenyl-acryloylamino) -4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine- (6S) -carboxylic acid ethyl ester (95) was prepared from compound 94 as described in in Example 5 for compound 3, 4.5 g (30%) of the title compound was obtained: ¹H NMR (CD3OD) d 1.3 (t, 3H), 2.35 (m, 1H),

2.65 (m, 1H), 3.1 (m, 1H), 3.15 (m, 1H), 4.25 (q, 2H), 5.15 (dd, 1H), 6.95 (d, 1H ), 7.4 (m, 3H), 7.6 (m, 2H),

7.65 (d, 1H), 8.95 (s, 1H).

4-Oxo-3- (2-phenyl-acryloylamino) -4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine- (6S) -carboxylic acid (96). A mixture of 4-Oxo-3- (3-phenyl-acryloylamino) -4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carboxylic acid ethyl ester (95.3.1 g , 8.8 mmol) and a 1N aqueous lithium hydroxide solution (8.8 ml, 8.8 mmol) in methanol (10 ml) was stirred for 18 hours at room temperature. The mixture was diluted with water and washed with diethyl ether (1 x 20 mL). The aqueous layer was acidified with concentrated hydrochloric acid. The solid was collected by filtration and washed with water, dried in a vacuum oven at 50 ° C for 18 hours to give 2.2 g (75%) of the title compound as a tan solid:¹H NMR (CD 3 OD) d 2.4 (m, 1H), 2.7 (m, 1H), 3.1 (m, 1H), 3.2 (m, 1H), 5.15 (dd, 1H) , 7.0 (d, 1H), 7.4 (m, 3H), 7.6 (m, 2H), 7.65 (d, 1H), 8.95 (s, 1H).

4-Oxo-3- (2-phenyl-acryloylamino) -4,6,7,8-tetrahydropyrrolo [1,2-] (2-benzyloxy-5-oxo-tetrahydrofuran- (3S) -yl) -amide. a] pyrimidine- (6S) -carboxylic acid (97) was prepared from compound 96 as described in Example 3 for compound H, step A to give 0.52 g (75%) of the title compound as a mixture of diastereomers: ¹H NMR (CDCl3) d 2.3-2.7 (m, 3H), 2.9 (dd, 1H), 3.05 (m, 1H), 3.3 (m, 1H), 4.4- 4.8 (m, 2H), 4.2 (2x d, 1H), 5.05 (m, 1H), 5.55 (2x s, 1H), 6.6 (2x d, 1H), 7, 4 (m, 6H), 7.55 (m, 4H), 7.65 (2xd, 1H), 8.0 (m, 2H), 9.2 (sx2.1H).

Acid - (3S) - {[4-oxo-3- (3-phenyl-propionylamino) -4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carbonyl] -amino acid } -butyric (98) was prepared as described in Example 3 for H, step D, to give 0.13 g (45%) of the title compound: ¹H NMR (CD3OD) d2.35 (m, 1H), 2.45-2.75 (m, 3H), 2.8 (t, 2H), 3.0 (t, 2H), 3.1 (m , 1H), 3.25 (m, 1H), 4.3 (m, 1H), 6.65 (dd, 1H), 5.15 (m, 1H), 7.15 (m, 1H), 7 . 3 (m, 4H), 8.8 (m, 1H).

<img file="PL193391B1_D0098.tif" />

3 (S) - (allyloxycarbonyl) -amino-4 - [(2,6-dichlorophenyl) -oxazol-2-yl] -4 (R, S) -hydroxybutyric acid tert-butyl ester (99). A solution of 5- (2,6-dichlorophenyl) oxazole (2.71 g, 12.7 mmol; prepared by a similar method to that described in Tet. Lett. 23, p. 2369 (1972)) in tetrahydrofuran (65 mL) was cooled to - 78 ° C under nitrogen atmosphere. To this solution was added n-butyllithium (1.5 M hexanes, 8.5 mL, 13.3 mmol) and stirred at -78 ° C for 30 minutes. Magnesium bromide etherate was added and the solution was allowed to warm to -45 ° C for 15 minutes. The solution was cooled to -78 ° C and aldehyde 58 (3.26 g, 12.7 mmol; Graybill et al., Int. J. Protein Res., 44, pp. 173-82 (1993)) in tetrahydrofuran (65) was added dropwise. ml). The reaction mixture was stirred for 25 minutes then allowed to warm to -40 ° C for 3 hours and then at room temperature for 1 hour. The reaction was quenched with 5% NaHCO3 (12 mL) and stirred for 3 hours. Tetrahydrofuran was removed in vacuo and the resulting residue was extracted with dichloromethane. The organic layer was washed with saturated sodium chloride solution, dried over magnesium sulfate, filtered and concentrated to provide 6.14 g of the title compound. After purification, 4.79 g (80%) of compound 99 was obtained:¹H NMR (CDCl3) d2.7-2.5 (m, 2H), 2.8 (dd, 1H), 4.2, 4.4 (2 xd, 1H), 4.7-4.5 (m , 3H), 5.35-5.1 (m, 2H), 5.6, 5.7 (2xd, 1H), 6.0-5.8 (m, 1H), 7.2 (d, 1H), 7.3 (m, 1H), 7.4 (m, 2H).

4-Oxo-3- (3-phenyl-propionylamino) -4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carboxylic acid (100). A mixture of 4-oxo-3- (3-phenyl-acryloylamino) -4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carboxylic acid (96, 2.1 g, 6 , 5 mmol) and 20% palladium hydroxide on carbon (0.5 g) in methanol (50 ml) was stirred under a hydrogen atmosphere for 4 hours. The resulting mixture was filtered and concentrated to give 2.1 g (100%) of the title compound as a white solid:¹H NMR (CD3OD) d2.35 (m, 1H), 2.65 (m, 1H), 2.75 (t, 2H), 3.0 (t, 2H), 3.1 (m, 1H), 3.15 (m, 1H), 5.1 (dd, 1H), 7.15 (m, 1H), 7.25 (m, 4H), 8.75 (s, 1H).

<img file="PL193391B1_D0099.tif" />

4-tert-butoxycarbonyl-2-oxo- (3S) - {[4-oxo-3- (3-phenyl-propionylamino) -4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine- 2,6-Dichloro-benzoic acid (6S) -carbonyl] amino} -butyl (101a) was prepared as described in Example 5 for compound 56a to give 0.16 g (20%) of the title compound: ¹H NMR (CD3OD) d 1.45 (s, 9H), 2.3 (m, 1H), 2.6 (m, 1H), 2.7 (m, 3H), 2.95 (m, 3H), 4.8 (m, 1H), 5.1 (m, 1H), 5.2 (q, 2H), 7.1 (m, 1H), 7.2 (m, 4H), 7.4 (m , 3H), 8.75 (s, 1H).

4- (7-Methoxy-benzoxazol-2-yl) -4-oxo- (3S) - {[4-oxo-3- (3-phenyl-1-propionylamino) -4,6,7 acid tert-butyl ester , 8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carbonyl] amino} -butyric (101b). 4-Hydroxy-4- (7-methoxy-benzoxazol-2-yl) - (3S) - {[4-oxo-3- (3-phenyl-1-propionylamino) -4,6,7 acid tert-butyl ester 8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carbonyl] amino} -butyric was prepared from compound 100 and 66a as described in Example 5 for compound 67a, yielding 0.95 g (quantitative) of the product as a mixture of diastereomers: ¹H NMR (CD3OD) d 1.45 (2xs, 9H), 2.2 (2xm, 1H), 2.35-3.0 (m, 9H), 4.0 (m, 3H), 4. 75 (m, 1H), 4.85 (m, 1H), 5.05 (2 x dd, 1H), 7.1 (2 x dd, 1H), 7.15-7.3 (m, 4H) , 7.5 (2xt, 1H), 7.8 (2xd, 1H), 8.55 (2xdd, 1H), 8.7 (2xs, 1H).

The resulting product was converted to compound 101b as described in Example 5 for compound 68a to obtain 0.36 g (50%) of the title compound: ¹H NMR (CD3OD) d1.4 (s, 9H), 2.35 (m, 1H), 2.55 (m, 1H), 2.75 (t, 2H), 2.95 (t, 2H), 3.00 (m, 1H), 3.1 (dd, 2H), 3.15 (m, 1H), 5.15 (dd, 1H), 5.65 (t, 1H), 7.1 (m , 2H), 7.2 (m, 4H), 7.4 (m, 2H), 8.7 (s, 1H).

4- [5- (2,6-Dichloro-phenyl) -oxazol-2-yl] -4-oxo- (3S) - {[4-oxo-3- (3-phenyl-1-] acid tert-butyl ester propionylamino) -4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine- (6S) carbonyl] amino} butyric (101c). 4- [5- (2,6-Dichloro-phenyl) -oxazol-2-yl] -4-hydroxy- (3S) - {[4-oxo-3- (3-phenyl-1- propionylamino) -4,6,7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carbo86

The methyl] amino} butyric acid was prepared from compounds 100 and 99 using the method described in Example 5 for compound 67a. 0.09 g (60%) of the product was obtained in the form of a mixture of diastereomers:¹H NMR (CD3OD) d 1.45 (2xs, 9H), 2.2 (m, 1H), 2.5 (m, 2H), 2.7 (2xdd, 1H), 2.75 (t , 2H), 2.9-3.1 (m, 4H), 4.7 (m, 1H), 5.1 (m, 1H), 7.1 (m, 1H), 7.1-7. 25 (m, 4H), 7.4 (t, 1H), 7.5 (t, 1H), 8.55 (d, 1H), 8.75 (s, 1H).

The resulting product was converted to compound 101c as described in Example 5 for compound 68a to give 0.04 g (45%) of the title compound: ¹H NMR (CD3OD) d 1.4 (s, 9H), 2.3 (m, 1H), 2.6 (m, 1H), 2.75 (t, 2H), 2.95 (t, 2H) , 2.9-3.2 (m, 4H), 5.2 (dd, 1H), 5.55 (t, 1H), 7.1 (m, 1H), 7.25 (m, 4H), 7.55 (m, 3H), 8.75 (s, 1H).

4-carboxy-2-oxo- (3S) - {[4-oxo-3- (3-phenyl-propionylamino) -4,6,7,8-tetrahydropyrrolo [1,2-a] pyrimidine- ( 2,6-Dichloro-benzoic acid 6S) -carbonyl] amino} -butyl (102a) was prepared from 101 as described in Example 5 for 57a to give 0.12 g (80%) of the title compound: ¹H NMR (CD3OD) d 2.35 (m, 1H), 2.65 (m, 1H), 2.75 (m, 2H), 2.85 (dd, 1H), 2.95 (m, 2H) , 3.0 (dd, 1H), 3.15 (m, 1H), 3.25 (m, 1H), 4.55 (dd, 1H), 5.15 (m, 1H), 5.25 ( q, 2H), 7.15 (m, 1H), 7.25 (m, 4H), 7.45 (m, 1H), 8.8 (s, 1H).

4- (7-Methoxy-benzoxazol-2-yl) -4-oxo- (3S) - {[4-oxo-3- (3-phenyl-propionylamino) -4,6,7,8-tetrahydropyrrolo acid [1,2-a] pyrimidine- (6S) -carbonyl] amino} -butyric (102b) was prepared from 101b as described in Example 5 for 69a and 0.12 g (35%) of the title compound was obtained: ¹H NMR (DMSO-d6) d 2.1 (m, 1H), 2.55 (m, 1H), 2.7-3.1 (m, 8H), 4.05 (s, 3H), 5, 1 (dd, 1H), 5.55 (t, 1H), 7.2 (m, 1H), 7.25 (m, 5H), 7.5 (t, 1H), 7.55 (d, 1H ), 8.7 (s, 1H), 9.2 (d, 1H), 9.4 (s, 1H), 12.7 (br, 1H).

4- [5- (2,6-Dichloro-phenyl) -oxazol-2-yl] -4-oxo- (3S) - {[4-oxo-3- (3-phenyl-propionylamino) -4.6 acid , 7,8-tetrahydro-pyrrolo [1,2-a] pyrimidine- (6S) -carbonyl] amino} -butyric (102c) was prepared from 101c as described in Example 5 for 69a afforded 0.01 g. (40%) of the title compound: ¹HNMR (CD3OD) d 2.35 (m, 1H), 2.6 (m, 1H), 2.75 (t, 2H), 2.95 (t, 2H), 3.05 (m, 1H), 3.15 (m, 3H), 5.15 (dd, 1H), 5.55 (t, 1H), 7.15 (m, 1H), 7.2 (m, 4H), 7.55 (m , 3H). 8.8 (s, 1H).

<img file="PL193391B1_D0100.tif" />

(3-tert-Butoxycarbonylamino-2-oxo-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl) -acetic acid methyl ester (103).

Step A. 2 (S) -tert-Butoxycarbonylamino-3- (2-nitrophenyl-amino) -propionic acid. Acid

2-tert-butoxycarbonylamino-3-aminopropionic (10 g, 49 mmol), 2-fluoronitrobenzene (5.7 ml, 54 mmol) and sodium bicarbonate (8.25 g, 98 mmol) were taken up in 130 ml of dimethylformamide and heated to temperature. 80 ° C for 18 hours. The mixture was evaporated in vacuo to give a sticky orange colored residue which was dissolved in 300 ml of water and extracted with diethyl ether (3 x 150 ml). The aqueous solution was acidified to pH 5 with 10% sodium bisulfate solution and extracted with ethyl acetate (3 x 250 ml). The combined extracts were dried over anhydrous sodium sulfate, filtered and evaporated to yield 12.64 g (83%) of the title compound as an orange amorphous solid.¹H NMR (CD3OD) d 8.15-8.10 (1H, d), 7.54-7.48 (1H, t), 7.13-7.08 (1H, d), 6.73-6 , 65 (1H, t), 4.45-4.35 (1H, m), 3.9-3.8 (1H, dd), 3.65-3.55 (1H, dd), 1.45 (9H, s).

Step B. 2 (S) -tert-butoxycarbonylamino-3- (2-aminophenylamino) -propionic acid.

A mixture of 2-tert-butoxycarbonylamino-3- (2-nitrophenylamino) propionic acid (12.65 g, 40.5 mmol) and 0.5 g of 10% Pd / C in 100 ml of methanol was stirred at 1 atm. hydrogen for 4 hours. The solution was filtered through Celite 545 and the filtrate was evaporated in vacuo to yield 11.95 g

Of title compound in quantitative yield as a dark brown solid that was used without purification. ¹H NMR (CD3OD) d6.75-6.70 (3H, m), 6.65-6.58 (1H, m), 4.35-4.3 (1H, m), 3.6-3. 38 (2H, m), 1.45 (9H, s).

Step C. 3 (S) -tert-butoxycarbonylamino-1,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-2-one. 1- (3-Dimethylaminopropyl) -3- hydrochloride was added to a cooled (0 ° C) solution of 2-tert-butoxycarbonylamino-3- (2-aminophenylamino) propionic acid (11.95 g, 40.5 mmol) in 100 ml of dimethylformamide. ethylcarbodiimide (8.54 g, 44.5 mmol) and stirred for 18 hours. The mixture was poured into 700 ml of ethyl acetate and washed four times with 100 ml of water. The organic layer was dried over anhydrous sodium sulfate, filtered and evaporated to give a brown solid which was purified by flash chromatography eluting with a 3: 7 mixture of ethyl acetate and hexane to give 8 g (71%) of the title compound: ¹H NMR (CDCl3) d 7.78 (1H, s), 7.02-6.95 (1H, m), 7.02-6.95 (1H, m), 6.88-6.82 (1H , m), 6.82-6.78 (IH, m), 6.75-6.70 (IH, m), 5.8-5.7 (IH, d), 4.55-4.45 (1H, m), 3.95 (1H, s), 3.9-3.82 (1H, m), 3.48-3.40 (1H, m), 1.45 (9H, s).

Step D. (3 (S) -T-butoxycarbonylamino-2-oxo-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl) -acetic acid methyl ester (103). To a cooled to -78 ° C solution of 3-tert-butoxycarbonylamino-1,3,4,5-tetrahydrobenzo [b] [1,4] diazepin-2-one (0.94 g, 3.38 mmol) in 20 ml anhydrous tetrahydrofuran, a solution of lithium bis (trimethylsilyl) amide (3.4 mL, 3.4 mmol) in THF was added dropwise and stirred for 30 minutes. Methyl bromoacetate (0.44 mL, 4 mmol) was added dropwise to the reaction mixture, and then the mixture was warmed to room temperature. The mixture was diluted with 100 ml of ethyl acetate and washed with 0.3N potassium hydrogen sulfate solution (50 ml), water (2 x 50 ml) and brine. The combined organics were dried over anhydrous sodium sulfate, filtered and evaporated to give a gum which was purified by flash chromatography eluting with a 3: 7 mixture of ethyl acetate and hexane. 0.98 g (83%) of the title compound was obtained as a white solid. ¹H NMR (CDCl3) d 7.15-7.07 (2H, m), 6.98-6.94 (1H, m), 6.88-6.84 (1H, m), 5.62-5, 55 (1H, d),

4.71-4.65 (1H, d), 4.65-4.6 (1H, m), 4.33-4.27 (1H, d), 3.96-3.90 (1H, m ), 3.78 (3H, s), 3.44-3.37 (1H, m), 1.4 (9H, s).

<img file="PL193391B1_D0101.tif" />

a R = Η b R = COCH₂CH₂Ph c R = CH₂Ph

[2-Oxo-3 (S) - (3-phenyl-propionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] -acetic acid methyl ester (104a). A solution of (3 (S) -tert-butoxycarbonylamino-2-oxo-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl) -acetic acid methyl ester (103.1 g, 2.86mmol) in 25 ml of ethyl acetate was bubbled with anhydrous hydrogen chloride for 2 minutes, followed by stirring.

The reaction was carried out for 1 hour at room temperature. The solution was evaporated to give 2-oxo-3 (S) -amino-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-ylacetic acid methyl ester hydrochloride as a white solid. The hydrochloride salt and hydrocinnamic acid (0.47 g, 3.15 mmol) were dissolved in 20 mL of dimethylformamide and cooled to 0 ° C. To the solution was added diisopropylethylamine (1 mL, 5.72 mmol) followed by N-hydroxybenzotriazole and 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride. After stirring for 18 hours at room temperature, the mixture was diluted with 150 ml of ethyl acetate and washed with 10% sodium bisulfate, 10% sodium bicarbonate solution and brine. The organic layer was dried over anhydrous sodium sulfate, filtered and evaporated to a crude solid which was purified by flash chromatography eluting with a 7: 3 mixture of ethyl acetate and dichloromethane to give 600 mg (55%) of the title compound as a white solid: ¹H NMR (CDCl3) d 7.3-6.85 (9H, m), 6.55-6.0 (1H, d), 4.88-4.82 (1H, m),

4.72-4.65 (1H, d), 4.28-4.22 (1H, m), 3.95-3.9 (1H, m), 3.78 (3H, s), 3. 65 (1H, br. S), 3.28-3.2 (1H, m), 2.95-2.84 (2H, m), 2.55-2.4 (2H, m).

(3 (S) - (3-Phenylpropionylamino) -2-oxo-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl) acetic acid (105a). (3 (S) - (3-Phenyl-propionylamino) -2-oxo-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl) -acetic acid methyl ester (104a) was dissolved in 90% methanol. Lithium hydroxide hydrate was added to the reaction mixture and stirred at room temperature for 4 hours. The mixture was evaporated in vacuo to give a white solid. This material was dissolved in 20 mL of water, acidified to pH 5, and extracted with ethyl acetate. 304 mg (88%) of the title compound was obtained as a white solid.¹HNMR (CDCl3) d 7.5-6.9 (11H, m), 4.92-4.8 (1H, m), 4.7-4.58 (1H, m), 4.38-4. 25 (1H, d), 3.88-3.78 (1H, m), 3.45-3.25 (1H, m), 3.05-2.85 (2H, m), 2.55- 2.45 (2H, m).

4-oxo-3 (S) - {2- [2-oxo-3 (S) - (3-phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepine- acid 1-ylacetylamino} butyric (106a). N- [1- (2-benzyloxy-5-oxotetrahydrofuran-3-ylcarbamoylmethyl) -2-oxo-2,3,4,5-tetrahydro-1H-benzo [b] [1,4] diazepin-3- yl] -3-phenylpropionamide was prepared from 105a as described in Example 3 for H (step A). 390 mg (93%) of the product were obtained in the form of diastereomers.¹H NMR (CD3OD) d 7.58-7.22 (14H, m), 5.78-5.73 (0.5H, d), 5.64 (0.5H, s), 5.0-4 , 72 (4H, m), 4.54-4.42 (2H, m), 3.82-3.76 (0.5H, m), 3.68-3.62 (0.5H, m) , 3.28-3.21 (0.5H, m), 3.19-3.12 (0.5H, m), 3.07-2.98 (2H, m), 2.78-2. 48 (4H, m).

The resulting product was converted to compound 106a as described in Example 3, compound H (Step D) to afford the title compound as a white solid (17%): ¹H NMR (CD3OD) d 7.54-6.98 (9H, m), 5.58-5.54 (1H, m), 4.8-4.2 (4H, m), 3.96-3 , 3 (2H, m), 3.30-3.05 (1H, m), 2.98-2.25 (5H, m).

[2-Oxo-5- (3-phenyl-propionyl) -3 (S) - (3-phenyl-propionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] acid methyl ester acetic acid (104b). A solution of (3 (S) -tert-butoxycarbonylamino-2-oxo-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl) -acetic acid methyl ester (103.1 g, 2, 86 mmol) in 25 ml of ethyl acetate was bubbled with anhydrous hydrogen chloride for 2 minutes and then stirred for 1 hour at room temperature. The mixture was evaporated to give 2-oxo-3 (S) -amino-2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-ylacetic methyl ester hydrochloride as a white solid. This hydrochloride was suspended in 20 ml of dichloromethane and cooled to 0 ° C. Triethylamine (1.6 mL, 11.5 mmol) was added to the suspension followed by the dropwise addition of dihydrocinnamoyl chloride (0.9 mL, 6 mmol) dropwise. The mixture was warmed to room temperature for 18 hours, diluted with 25 mL of dichloromethane and washed twice with 50 mL of water and once with 50 mL of brine. The organic layer was dried over anhydrous sodium sulfate, filtered and evaporated. A viscous yellow oil was obtained which was purified by flash chromatography eluting with a 1: 1 mixture of ethyl acetate and dichloromethane to give 1.35 g (92%) of the title compound as a white solid.¹H NMR (CDCl3) d 7.45-7.02 (14H, m), 6.37-6.32 (1H, d), 4.78-4.72 (1H, m), 4.52-4 , 3 (3H, m), 3.82-3.77 (1H, m), 3.74 (3H, s), 3.03-2.87 (4H, m), 2.58-2.45 (2H, m), 2.45-2.35 (IH, m), 2.25-2.16 (IH, m).

[2-Oxo-5- (3-phenylpropionyl) -3- (3 (S) -phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] acetic acid ( 105b). [2-Oxo-5- (3-phenyl-propionyl) -3- (3-phenyl-propionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] -acetic acid methyl ester (104b , 680 mg, 1.32 mmol) was hydrolyzed as in Example 105a to yield 645 mg (98%) of the title compound as a white solid.¹H NMR (CDCl3) d 7.58 (1H, br s), 7.5-7.42 (1H, m), 7.35-6.95 (14H, m), 4.95-4.88 (1H, m), 4.64-4.55 (1H, d), 4.54-4.45 (1H, t), 4.15-4.05 (1H, d), 3.75 (1H , m), 3.05-2.75 (4H, m), 2.58-2.45 (2H, m), 2.45-2.28 (1H, m), 2.25-2.14 (1H, m).

PL 193 391 B1

2-Oxo-3 (S) - {2- [2-oxo-5- (3-phenylpropionyl) -3 (S) - (3-phenylpropionylamino) -2,3,4,5-tetrahydrobenzo [b] [ 1,4] diazepin-1-yl] acetylamino} butyric (106b). [2-oxo-5- (3-phenylpropionyl) -3- (3-phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] acetic acid and tert ester semicarbazone -butyl 3-amino-4-oxobutyric acid was coupled as described in Example 3 for compound K (step A) to give 350 mg (85%) of a white solid.¹H NMR (CDCl3) d 9.05 (1H, br. S), 7.58-7.55 (1H, d), 7.5-7.35 (1H, m), 7.35-6.95 (14H, m), 6.75-6.72 (1H, d), 6.25 (1H, br. S), 5.25 (1H, br. S), 4.95-4.88 (1H , m), 4.8-4.72 (1H, m), 4.55-4.4 (2H, m), 3.92-3.88 (1H, d),

3.73-3.68 (1H, m), 2.95-2.8 (4H, m), 2.8-2.72 (1H, m), 2.62-2.55 (1H, m ), 2.55-2.45 (2H, m), 2.4-2.32 (1H, m), 2.2-2.12 (1H, m), 1.45 (9H, s).

By the method described in Example 3, compound K (step C) from 4-oxo-3- {2- [2-oxo-5- (3-phenylpropionyl) -3- (3-phenylpropionylamino) acid tert-butyl ester semicarbazone The 3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] -acetylamino} butyric group was removed to give 118 mg (47%) of the title compound as a white solid. ¹H NMR (CD3OD) d 7.48-6.95 (14H, m), 4.65-4.15 (6H, m), 3.5-3.4 (1H, m), 2.85-2 , 72 (4H, m), 2.65-2.5 (1H, m), 2.5-2.34 (3H, m), 2.34-2.15 (2H, m).

[5-Benzyl-2-oxo-3 (S) - (3-phenyl-propionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] -acetic acid methyl ester (104c) . [2-Oxo-3- (3-phenyl-propionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] -acetic acid methyl ester (104a, 500 mg, 1.31 mmol ), calcium carbonate (155 mg, 1.58 mmol) and benzyl bromide (170 ml 1.44 mmol) were taken up in 10 ml dimethylformamide and heated to 80 ° C for 8 hours. The mixture was diluted with 150 ml of ethyl acetate and washed 4 times with 50 ml of water. The organic layer was dried over anhydrous sodium sulfate, filtered and evaporated. A viscous yellow oil was obtained which was purified by flash chromatography eluting with a mixture of dichloromethane and ethyl acetate (8: 2) to give 460 mg (75%) of the title compound as a white solid.¹HNMR (CDCl3) d 7.34-7.05 (14H, m), 6.32-6.28 (1H, d), 4.84-4.76 (1H, d), 4.76-4, 70 (1H, m), 4.43-4.37 (1H, d), 4.26-4.18 (1H, d), 4.06-4.00 (1H, d), 3.79 ( 3H, s), 3.45-3.37 (1H, m), 3.02-2.95 (1H, m), 2.90-2.82 (2H, m), 2.5-2. 34 (2H, m).

[5-Benzyl-2-oxo-3 (S) - (3-phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] acetic acid (105c) prepared by hydrolysis of the ester (102c) as described for 105a to give 450 mg (98%) of the title compound as a white solid. ¹H NMR (CD3OD) d 7.5-7.05 (14H, m), 6.4 (1H, br. S), 4.85-4.55 (2H, m), 4.5-4.21 (2H, m), 4.12-3.92 (1H, d), 3.45-3.3 (1H, m), 3.1-2.8 (3H, m), 2.55-2 . 28 (3H, m).

3 (S) - {2- [5-benzyl-2-oxo-3- (3 (S) -phenyl-propionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1- acid yl] -acetylamino} -4-oxobutyric (106c). [5-benzyl-2-oxo-3 (S) - (3-phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] acetic acid and tert-ester semicarbazone 3 (S) -amino-4-oxobutyric acid butyl was coupled as in Example 3, compound K (step A) to afford 260 mg (85%) of a white solid.¹H NMR (CD3OD) d 7.35-7.0 (15H, m), 4.94-4.88 (1H, m), 4.68-4.58 (1H, d), 4.57-4 , 52 (1H, m), 4.41-4.34 (1H, d), 4.3-4.23 (1H, d), 4.1-4.04 (1H, d), 3.18 -3.11 (1H, m), 3.09-2.98 (1H, m), 2.78-2.72 (2H, t), 2.65-2.57 (1H, m), 2 , 42-2.33 (3H, m).

By the method described in Example 3, compound K (step C) from 3 (S) - {2- [5-benzyl-2-oxo-3 (S) - (3-phenylpropionylamino) acid tert-butyl ester semicarbazone -2.3 The 4,5-tetrahydro-benzo [b] [1,4] -diazepin-1-yl] acetylamino} -4-oxobutyric group was removed to give 168 mg (81%) of the title compound as a white solid. ¹HNMR (CD3OD) d 7.37-7.0 (14H, m), 4.75-4.62 (1H, m), 4.6-4.45 (2H, m), 4.4-4. 21 (2H, m), 4.15-3.95 (2H, m), 3.15-3.0 (2H, m), 2.82-2.67 (2H, m), 2.65- 2.52 (1H, m), 2.5-2.32 (3H, m).

PL 193 391 B1

<img file="PL193391B1_D0102.tif" />

4-tert-Butoxycarbonyl-2-oxo-3 (S) - {2- [2-oxo-5- (3-phenylpropionyl) -3 (S) - (3-phenylpropionylamino) -2,3,4,5 ester -tetrahydrobenzo [b] [1,4] diazepin-1-yl] acetylamino} butyl 2,6-dichlorobenzoic acid (107a). The obtained semicarbazone was prepared by coupling 105b and t-butyl 3- (allyloxycarbonylamino) -4-oxo-5- (2,6-dichlorobenzoyloxy) pentanoate (WO 93 16710) as described for 56a to afford 256 mg (58% ) title compound as a white solid.¹H NMR (CDCl3) d 7.45-7.04 (17H, m), 6.45-6.34 (2H, m), 5.28-5.21 (1H, m), 5.1-5, 0 (1H, m), 4.95-4.90 (1H, m), 4.75-4.70 (1H, m), 4.55-4.44 (1H, m), 4.32- 4.22 (1H, dd), 3.99-3.85 (1H, dd), 3.85-3.76 (1H, m), 3.06-2.83 (5H, m), 2, 83-2.74 (1H, m), 2.6-2.44 (2H, m), 2.43-2.33 (1H, m), 2.24-2.15 (1H, m), 1.45 (9H, s).

4-carboxy-2-oxo-3 (S) - {2- [2-oxo-5- (3-phenylpropionyl) -3 (S) - (3-phenylpropionylamino) -2,3,4,5-tetrahydro-benzo ester 2,6-Dichlorobenzoic acid [b] [1,4] diazepin-1-yl] acetyl-amino} butyl (108a) was prepared from 107a as described for 57a to afford 156 mg (68%) of the title compound as white solid. ¹H NMR (CD3OD) d 7.5-7.9 (17H, m), 5.16-5.02 (1H, dd), 4.88-4.71 (2H, m), 4.62-4 , 44 (2H, m), 4.42-4.28 (2H, m), 4.27-4.18 (1H, m), 3.47-3.41 (1H, m), 2.90 -2.60 (5H, m), 2.46-2.4 (2H, m), 2.39-2.18 (2H, m).

4- (7-Methoxy-benzoxazol-2-yl) -4-oxo-3 (S) - {2- [2-oxo-5- (3-phenyl-propionyl) -3 (S) - (3- phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] acetylamino} butyric (107b). 4 (R, S) -hydroxy-4- (7-methoxy-benzoxazol-2-yl-3 (S) - {2- [2-oxo-5- (3-phenyl-propionyl) -3 (S) acid tert-butyl ester - (3-phenylpropionylamino) -2,3,4,5-tetrahydro [b] [1,4] diazepin-1-yl-acetylamino} butyric was prepared from 105b and 66a as described in Example 5 for compound 67, and 56% white solid was obtained. ¹H NMR (CDCl3) d 7.72-6.78 (19H, m), 6.37-6.28 (1H, m), 5.17-5.08 (0.5H, m), 4.92 -4.82 (0.5H, m), 4.81-4.6 (1H, m), 4.6-4.35 (3H, m), 4.05-3.9 (1H, m) , 3.95 (3H, s), 3.82-3.7 (1H, m), 2.96-2.05 (10H, m), 1.45 (4.5H, s), 1.38 (4.5H, s).

The resulting product was converted to compound 107b as described in Example 5 for compound 69a to afford the title compound (56%) as a white solid. ¹H NMR (CD3OD) d 7.62-6.8 (17H, m), 5.64-5.58 (0.5H, t), 5.52-5.46 (0.5H, t), 4 , 62-4.47 (2H, m), 4.40-4.32 (1H, m), 3.9 (1.5H, s), 3.88 (1.5H, s), 3.43 -3.37 (1H, m), 3.0-2.92 (1H, m), 2.90-2.62 (6H, m), 2.5-2.4 (2H, m), 2 , 28-2.15 (2H, m), 1.32 (4.5H, s), 1.25 (4.5H, s).

4- (7-methoxybenzoxazol-2-yl) -4-oxo-3 (S) - {2- [2-oxo-5- (3-phenylpropionyl) -3 (S) - (3-phenylpropionylamino) -2 acid , 3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] acetylamino} butyric (108b) was prepared as described in Example 5 for 69a to afford the title compound (50%) as white solid. ¹H NMR (CD3OD) d 7.41-6.88 (17H, m), 5.6-5.55 (0.5H, t), 5.48-5.43 (0.5H, t), 4 , 64-4.45 (2H, m), 4.45-4.30 (1H, m), 3.93 (1.5H, s), 3.90 (1.5H, s), 3.47 -3.34 (1H, m), 3.10-2.85 (2H, m), 2.84-2.63 (5H, m), 2.6-2.4 (2H, m), 2 , 3-2.1 (2H, m).

PL 193 391 B1

4- [5- (2,6-Dichloro-phenyl) oxazol-2-yl) -4-oxo-3 (S) - {2- [2-oxo-5- (3-phenyl-propionyl) -3 acid tert-butyl ester (S) - (3-phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] 1,4] diazepin-1-yl] acetylamino} butyric (107c). 4- [5- (2,6-Dichloro-phenyl) oxazol-2-yl] -4 (R, S) -hydroxy-3 (S) - {2- [2-oxo-5- (3 -phenylpropinyl) -3 (S) - (3-phenylpropionylamino) -2,3,4,5-tetrahydrobenzo [b] [1,4] diazepin-1-yl] -acetylamino} butyric acid was prepared from compounds 106c and 99 by similar to compound 67a in Example 5 gave 72% white solid. ¹H NMR (CDCl3) d 7.71-7.64 (1H, m), 7.58-7.42 (2H, m), 7.42-6.92 (15H, m), 6.5-6 , 37 (2H, m), 5.15-5.04 (1H, m), 4.88-4.68 (2H, m), 4.57-4.37 (2H, m), 4.28 -4.13 (1H, m), 3.87-3.64 (2H, m), 3.04-2.80 (4H, m), 2.76-2.68 (1H, m), 2 , 67-2.42 (3H, m), 2.41-2.31 (1H, m), 2.22-2.12 (1H, m), 1.45 (9H, s).

The resulting product was converted to compound 107c in a similar procedure to that described for compound 68a in Example 5 to give the title compound in quantitative yield as a white solid. ¹H NMR (CDCl3) d 7.47-6.98 (18H, m), 6.52-6.42 (1H, d), 5.6-5.52 (1H, m), 4.78-4 , 71 (1H, m), 4.52-4.40 (2H, m), 4.03-3.94 (0.67H, m), 3.94-3.85 (0.33H, m) , 3.85-3.75 (1H, m), 3.45-3.33 (1H, m), 3.08-2.98 (1H, m), 2.97-2.84 (4H, m), 2.55-2.43 (2H, m), 2.43-2.32 (1H, m), 2.23-2.13 (1H, m), 1.35 (9H, s) .

4- [5- (2,6-Dichlorophenyl) oxazol-2-yl] -4-oxo-3 (S) - {2- [2-oxo-5- (3-phenylpropionyl) -3 (S) - acid (3-phenylpropionylamino) -2,3,4,5-tetrahydro-benzo [b] [1,4] diazepin-1-yl] -acetylamino} butyric (108c) was prepared from 107c, similar to 69a in Example 5, afforded 72% of the title compound as a white solid. ¹H NMR (CD3OD) d 7.58-7.0 (18H, m), 5.62-5.53 (0.67H, m), 5.52-5.47 (0.33H, m), 4 , 68 (3H, m), 3.54-3.42 (1H, m), 3.1-2.92 (2H, m), 2.88-2.68 (5H, m), 2.63 -2.45 (2H, m), 2.40-2.22 (2H, m).

<img file="PL193391B1_D0103.tif" />

PL 193 391 B1

3 (S) - {2 (R, S) - [4-benzyl-7-oxo-6 (S) - (N-benzyloxycarbonylamino) - [1,4-diazepan-1-yl] propionylamino} -4 acid salt -oxo butyric acid with trifluoroacetic acid (114a):

Stage. A. To a solution of tert-butyl 2-N-benzyloxycarbonyl-3-N-benzyl- (S) -2,3-diaminopropionate (110; 0.85 g, 2.2 mmol), 3- (N- tert-butoxycarbonyl) amino-2-methyl-5-oxo-pentanoic (109a; 0.65 g, 2.7 mmol), acetic acid (0.1 ml, 1.8 mmol), sodium acetate (0.36 g, 2 mmol) and 0.4 nm molecular sieves (1 g) in methanol (45 ml) were added sodium cyanoborohydride (0.33 g, 5.3 mmol). The mixture was stirred overnight at 25 ° C, then filtered through Celite and concentrated under reduced pressure. The residue was dissolved in 1N NaOH and extracted with ethyl acetate (3 x 40 ml). The organic layer was dried (MgSO4), filtered and evaporated to yield an oil. Chromatography (silica gel, 4: 1 hexane: ethyl acetate as eluent) gave 0.92 g (68% yield) of compound 111 as an oil.

Step B. The product obtained above was dissolved in chilled to 0 ° C dichloromethane (3 ml) and treated with a 25% solution of trifluoroacetic acid in dichloromethane (20 ml) then allowed to warm to 25 ° C and stirred until TLC indicated the reaction was complete (hexane: ethyl acetate, 4: 1). The solvent was removed under reduced pressure and the residue was dried in vacuo then dissolved in dichloromethane (40 mL) and treated with 4-methylmorpholine (1 mL, 9 mmol), HOBT (0.2 g, 1.5 mmol) and EDC (0 , 61 g, 3.2 mmol). The resulting mixture was stirred overnight at 25 ° C then diluted with dichloromethane and washed with water. The organic layer was dried (MgSO4), filtered and evaporated to yield an oil. Chromatography (silica gel, 3: 2 hexane: ethyl acetate as eluant) gave 0.49 g (74% yield) of 112a as a viscous oil.

Step C. A solution of 2 (R, S) - [4-benzyl-7-oxo-6 (S) - (N-benzyloxycarbonylamino) - [1,4] diazepan-1-yl} -propionic acid methyl ester (112a; 0.15 g, 0.32 mmol) was dissolved in methanol, treated with 1M LiOH (0.32 ml) and stirred for 5.5 hours at 25 ° C, then evaporated to dryness. The residue was azeotroped with ethanol (2 x 10 ml), acetonitrile (2 x 10 ml) and benzene (2 x 10 ml) and dried to dryness. The resulting residue was converted to compound 114a in a similar manner to Example 3 for compound K (steps A, B, and C), and then purified by reverse phase HPLC (C18 column) using 0.1% TFA: water / 0.1% TFA: acetonitrile. 17 mg (10% yield) of viscous oil were obtained:¹H NMR (500 MHz, CD 3 OD) d 1.15 (m, 3H), 2.30-2.70 (m, 6H), 2.72-2.95 (bm, 6H), 3.30-3.80 (m, 4H), 4.10 (m, 1H), 4.40 (m, 4H), 4.95 (m, 1H), 6.95-7.10 (bs, 5H) and 7.12- 7.20 ppm (bs, 5H).

3 (S) - {2- [4-benzyl-7-oxo-6 (S) - (N-benzyloxycarbonylamino) - [1,4] diazepan-1-yl] -acetylamino} -4-oxo-butyric acid salt with trifluoroacetic acid (114b) was prepared from 109b by a method similar to that described for the synthesis of 114a to give 85 mg of a viscous oil: ¹H NMR (500 MHz, CD 3 OD) d (d, J = 7 Hz, 3H), 2.28 (m, 2H), 2.60 (m, 2H), 3.18 (bs, 6H), 3.35 -3.45 (m, 2H), 3.60-3.95 (m, 2H), 4.15 (m, 1H), 4.32 (m, 1H), 4.42 (m, 1H), 5.00 (bm, 2H), 7.20 (bs, 5H), and 7.40 ppm (bs, 5H); ¹⁹F NMR (470 MHz, CD3OD) d10.72 ppm (s, 3 F).

4-Oxo-3 (S) - {2 (R, S) - [7-oxo-4- (3-phenyl-propionyl) -6 (S) - (3-phenyl-propionylamino) - [1.4 ] diazepan-1-yl] -propionylamino) -butyric (115):

Step D. Suspension of 2 (R, S) - [4-benzyl-7-oxo-6 (S) - (N-benzyloxycarbonylamino) - [1,4] diazepan-1-yl] propionic acid methyl ester (112b; 0 , 22 g, 0.49 mmol) and 20% Pd (OH) 2 on carbon (50 mL) in ethanol was stirred under a hydrogen atmosphere for 7 hours. The solvent was evaporated under reduced pressure and the residue was dissolved in dichloromethane (20 ml) and then treated with triethylamine (1 ml) and dihydrocinnamoyl chloride (170 mg, 1 mmol). The resulting mixture was stirred overnight then diluted with ethyl acetate and washed with 1N NaOH. The organic layer was dried (MgSO4), filtered and evaporated to yield an oil. Chromatography (silica gel, 4: 1 hexane: ethyl acetate) gave 0.175 g (75% yield) of compound 113 as an oil.

Step C. A 0.15 g portion of compound 113 (0.32 mmol) was dissolved in methanol, treated with 1M LiOH (0.32 ml), stirred at 40 ° C overnight and then evaporated to dryness. The residue was azeotroped with ethanol (2 x 10 ml), acetonitrile (2 x 10 ml), benzene (2 x 10 ml) then dried in vacuo. The resulting residue was converted to compound 115 in a similar manner to that described in Example 3 for compound K (steps A, B, and C).

PL 193 391 B1

<img file="PL193391B1_D0104.tif" />

3- {2- [2,4-Dibenzyl-3,7-dioxo-6- (N-benzyloxycarbonylamino) - [1,4] diazepan-1-yl] acetylamino} -4-oxobutyric acid (121):

Step E. A solution of tert-butyl 2-N-carbobenzoxy-3-N-benzyl- (S) -2,3-diaminopropionate (100;

1.77 g, 4.6 mmol), N-allyl-N-tert-butoxycarbonyl- (S) -phenylalanine (116; 1.04 g, 4.8 mmol), HOBT (0.74 g, 5.5 mmol) and EDC (1.33 g, 6.9 mmol) in dichloromethane (50 mL) was stirred at 25 ° C for 16 hours, then diluted with dichloromethane (100 mL) and washed with water. The organic layer was dried (MgSO4), filtered and evaporated to yield an oil. Chromatography (silica gel, hexane: ethyl acetate 85:15) gave 1.34 g (43% yield) of compound 117 as a colorless viscous oil.

Step F. 1.34 g of compound 117 was dissolved in dichloromethane (3 mL) and treated with a 50% solution of trifluoroacetic acid in dichloromethane (20 mL). After 1.5 hours, the solvent was removed in vacuo and the residue was dried in vacuo, then dissolved in dichloromethane (50 ml) and combined with 4-methylmorpholine (0.2 ml, 2 mmol), HOBT (0.27 g, 2 mmol). ) and EDC (0.8 g, 4 mmol). The mixture was stirred overnight at 25 ° C, then diluted with dichloromethane and washed with water. The organic layer was dried (MgSO4), filtered and evaporated to an oil. Chromatography (silica gel, hexane: ethyl acetate 7: 3) gave 0.8 g (80% yield) of compound 118 as a viscous oil.

Step G. A 0.8 g portion of compound 188 was dissolved in methanol (400 mL), cooled to -78 ° C, and saturated with ozone until the solution turned blue. Excess ozone was removed by purging with argon, then dimethyl sulfide (5 mL) was added and the mixture was allowed to warm to 25 ° C and stirred for 3 hours. Removal of the solvent and chromatography (silica gel, hexane: ethyl acetate, 1: 1) provided 0.74 g (74% yield) of compound 119 as a white solid.

PL 193 391 B1

Step H. A 0.2 g (0.4 mmol) of Compound 119 was dissolved in acetone (25 mL), cooled to 0 ° C, and Jones's reagent solution was added dropwise until the solution turned orange. 2-Propanol (5 ml) was then added to the mixture and the resulting solution was filtered through Celite and washed with acetone. Removal of the solvent gave a green / white solid which was dried in vacuo to yield compound 120. The resulting residue was converted to compound 121 in a similar manner to that described in Example 3 for compound K (steps A, B, and C). Chromatography (SiO2) with dichloromethane: methanol: acetic acid 95: 4.5: 0.5 as eluent) gave 85 mg (53% yield) of a cream-colored solid, identified as the acid 3- {2- [ 2,4-dibenzyl-3,7-dioxo-6- (N-benzyloxycarbonylamino) - [1,4] diazepan-1-yl-acetylamino} -4-oxo-butyric (121) based on the following spectral data: ¹H NMR (500 MHz, CD3OD) d 2.38 (m, 1H), 2.45 (m, 1H), 3.21 (bs, 2H), 3.32-3.39 (bm, 6H), 3 . 85 (m, 1H), 4.05 (m, 1H), 4.21 (bm, 1H), 4.31 (bs, 1H), 4.45 (dm, J = 11Hz, 1H), 4 , 95 (bs, 4H), 7.20 (bs, 5H) and 7.33-7.45 ppm (m, 5H); ¹⁹F NMR (470 MHz, CD3OD) d10.62 (s, 3F).

<img file="PL193391B1_D0105.tif" />

T-Butyl 3 (S) -N- (allyloxycarbonyl) -3-amino-5- (2-chlorophenylmethylthio) -4-oxo-pentanoate (123). To a solution of t-butyl 3 (S) N- (allyloxycarbonyl) -3-amino-5-bromo-4-oxo-pentanoate (122; 749 mg, 2.14 mmol; WO 93 16710) in dimethylformamide (20 ml) was added potassium fluoride (273 mg, 4.70 mmol) with stirring followed by 2-chlorophenylmethylthiol (373 mg, 2.35 mmol). The mixture was stirred for 3.5 hours, quenched with water (50 ml) and extracted with ethyl acetate (2 x 50 ml). The combined organic extracts were washed with water (4 x 50 ml) then brine (50 ml) then dried (MgSO4) and concentrated to give an oil which was purified by flash chromatography (10-35% ethyl acetate / hexane). 832 mg (91%) of a colorless solid were obtained: mp. 45-6 ° C; [a] D²⁰ -1 9.0 ° (c 1.0, CH2Cl2); IR (coating) 3340, 2980, 2935, 1725, 1712, 1511, 1503, 1474, 1446, 1421, 1393, 1281, 1244, 1157, 1052, 1040, 995, 764, 739;¹H NMR (CDCl3) d 7.36 (2H, m), 7.21 (2H, m), 5.91 (2H, m), 5.27 (2H, m), 4.76 (1H, m) , 4.59 (2H, d), 3.78 (2H, s), 3.36 (2H, m), 2.91 (1H, dd), 2.74 (1H, dd), 1.43 ( 9H, s). Analysis. Calculated for C20H26ClNO5S: C, 56.13; H, 6.12; N, 3.27; S, 7.49. Found C, 56.08; H, 6.11; N, 3.26; S, 7.54. MS (CI); 430/28 (M⁺+ 1,3%); 374/2 (100).

T-Butyl (3S) 3 (2 {6-benzyl-1,2-dihydro-2-oxo-3 (3-phenylpropionylamino) -1-pyridyl) acetylamino-5- (2-chlorophenylmethylthio) -4-oxopentanoate (124a). 6-Benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionylamino) -pyridyl acetic acid (52b; 300 mg, 0.76 mmol) in THF (7 mL) was mixed with 1-hydroxybenzotriazole (205 mg, 1.52 mmol) and 1- (3-dimethylaminopropoxy-3-ethyl-carbodiimide) hydrochloride. After 3 hours, water (12 drops) was added and the mixture was stirred for 10 minutes, then t-butyl (3S) N- (allyloxycarbonyl) -3-amino-5- (2-chlorophenylmethylthio) -4-oxopentanoate was added.

(123) (325 mg, 0.76 mmol), bis (triphenylphosphine) palladium II chloride (20 mg), and tributyltin hydride (0.6 mL, 2.28 mmol). The mixture was stirred for 5 hours at room temperature, poured into ethyl acetate and washed with aq. 1M HCl (x 2), aq. Sodium bicarbonate, brine, dried (MgSO4) and concentrated. The residue was triturated with pentane and the supernatant was discarded. Chromatography (silica gel, 50% ethyl acetate / hexane) gave a colorless foam (439 mg, 81%): [a] D²¹ -18.3 ° (c 0.5, CH2Cl2); IR (KBr) 3356, 3311, 1722, 1689, 1646, 1599, 1567, 1513, 1367, 1154;¹H NMR (CDCl 3) d 8.39 (1H, d), 8.23 ​​(1H, s), 7.24 (14H, m), 6.16 (1H, d), 4.95 (1H, m) , 4.63 (2H, m), 4.02 (2H, s), 3.74 (2H, s), 3.27 (2H, s), 2.85 (6H, m), 1.40 ( 9H, s). Analysis. Calculated for C39H42ClN3O6S: C, 65.39; H, 5.91; N, 5.87. Found C, 65.51; H, 5.99; N, 5.77.

[3S (1S, 9S)] - 3- (6.10-dioxo-1,2,3,4,7,8,9,10-octahydro) -9- (3-phenylpropionylamino) -6H-pyridazine- [ T-Butyl 1,2-a] [1,2] diazepine-1-carboxamido-5- (2-chlorophenylmethylthio) -4-oxopentanoate (124b) was prepared by a method similar to compound 124a from thioether 123 and 3S acid (1S, 9S ) -3- (6.10-dioxo-1,2,3,4,7,8,9,10-octahydro) -9- (3-phenylpropionylamino) -6H-pyridazine [1,2-a] [1 , 2] diazepino21

-1-carboxylic acid (45a) to give 452 mg (50%) of a colorless foam: mp. 55-7 ° C; [a] D²¹-94.0 ° (c 0.12, CH2Cl2); IR (KBr) 3288, 2934, 1741, 1722, 1686, 1666, 1523, 1433, 1260, 1225, 1146, 757;¹H NMR (CDCl3) d 7.35 (3H, m), 7.20 (7H, m), 6.46 (1H, d), 5.21 (1H, m), 4.97 (2H, m) , 4.56 (1H, m), 3.75 (2H, s), 3.25 (3H, m), 2.93 (5H, m), 2.71 (1H, dd), 2.55 ( 2H, m), 2.30 (1H, m), 1.92 (3H, m), 1.66 (2H, m), 1.42 (9H, s). Analysis. Calculated for C35H43ClN4O7S. 0.25H2O: C, 59.73; H, 6.23; Cl, 5.04; N, 7.96; S, 4.56. Found C, 59.73; H, 6.19; Cl, 5.10; N, 7.79; S, 4.58. MS (-FAB) 697 (M-1,100).

(3S) 3 (2 (6-Benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionylamino) -1-pyridyl) acetylamino-5- (2-chlorophenylmethylthio) -4-oxopentanoic acid (125a) T-Butyl 3 (2 (6-benzyl-1,2-dihydro-2-oxo-3- (3-phenylpropionylamino) -1-pyridyl) acetylamino-5- (2-chlorophenylmethylthio) -4-oxopentanoate ( 124a) (400 mg, 0.56 mmol) in dichloromethane (3 ml) was treated with trifluoroacetic acid (3 ml) at 0 ° C and stirred at 0 ° C for 1 hour and at room temperature for 0.5 hours. The solution was concentrated then redissolved in dichloromethane and concentrated again. The operation was repeated three times. The residue was stirred in ether for 1 hour and filtered to give a colorless solid (364 mg, 99%); mp temp. 165-7 ° C; [a] D²² - 27.7 ° (c 0.2, CH2Cl2); IR (KBr) 3289, 1712, 1682, 1657, 1645, 1593, 1562, 1527, 1497, 1416, 1203, 1182;¹H NMR (CDCl 3) d 8.47 (1H, d), 8.21 (1H, s), 7.70 (1H, d), 7.22 (14H, m), 6.24 (1H, d) , 5.03 (1H, m), 4.65 (2H, m), 4.06 (2H, s), 3.69 (2H, m), 3.23 (2H, m), 2.88 ( 6H, m),

Acid [3 (1S, 9S)] - 3- (6,10-dioxo-1,2,3,4,7,8,9,10-octahydro) -9- (3-phenylpropionylamino) -6H-pyridazine- [1,2-a] [1,2] diazepine-1-carboxamido-5- (2-chlorophenyl-methylthio) -4-oxopentane (125b) was prepared in a similar manner to that described for compound 125a from t-butyl ester 124b and 362 mg (93%) of a colorless powder were obtained: mp. 76-80 ° C; [a] D²¹ -134 ° (c 0.10, MeOH); IR (KBr) 3309, 2935, 1725, 1658, 1528, 1445, 1417, 1277, 1219, 1175;¹HNMR (D6-DMSO) d 8.80 (1H, d), 8.19 (1H, d), 7.31 (9H, m), 5.09 (1H, m), 4.74 (1H, m ), 4.63 (1H, m), 4.35 (1H, m), 3.76 (2H, m), 3.28 (3H, m), 2.80 (5H, m), 2.52 (4H, m), 2.16 (2H, m), 1.90 (3H, m). Analysis. Calculated for C31H35Cl2N4O7S. 0.25H2O: C, 57.49; H, 5.53; N, 8.65; S, 4.95. Found C, 57.35; H, 5.43; N, 8.45; S, 4.88.

The data from the examples above demonstrate that compounds of the invention exhibit inhibitory activity against IL-1b converting enzyme.

Since the compounds of the invention are capable of inhibiting ICE in vitro and, moreover, can be administered orally to mammals, they have obvious clinical utility in the treatment of IL-1 dependent diseases. From these assays, the ability of compounds to inhibit ICE in vivo can be predicted.

Notwithstanding the many embodiments of the present invention described, it is evident that the basic structure can be varied to provide other embodiments with the products and methods of the invention. Thus, it should be considered that the scope of the present invention is defined by the appended claims and not by the specific embodiments presented by way of illustration only.