ANTIGESTAGENIC STEROIDS WITH A FLUORINATED 17α -ALKYL CHAIN, pharmaceutic preparations containing them and their use

The present invention relates to 17-α fluoroalkyl steroids, pharmaceutical preparations containing them and their use as medicaments.

The invention relates to 17-α fluoroalkyl steroids of general formula I,

<img file="PL191808B1_D0001.tif" />

wherein:

₁

R¹ denotes a methyl or ethyl group, ₂

R² represents a residue of formula CnFmHo, with n being 2,3,4,5 or 6, m&gt; 1 and m + o = 2n + 1, ₃

R³ denotes a free, etherified or esterified hydroxyl group,

R⁴ and r⁵ each represents a hydrogen atom or together an additional bond or a methylene group, St is a steroid ABC ring system of partial formula A, B or C,

<img file="PL191808B1_D0002.tif" />

wherein:

R⁶ represents a hydrogen atom, a straight chain C1-C4 or branched C3-C4 alkyl group or a halogen atom,

R⁷ is a hydrogen atom, a straight chain C1-C4 or branched C3-C4 alkyl group, or if St is a steroidal ring system ABC A or B then R⁶ and r⁷ can together mean an additional bond,

X is oxygen, hydroxyimino = N ~ OH or two hydrogen atoms,

R⁸ represents a Y residue or an aryl residue optionally multisubstituted with a Y group, wherein Y is hydrogen, halogen, -OH, -NO2, -N3, -CN, -NR^9aR^9b, -NHSO2R⁹, -CO2R⁹, C1-C10-alkyl-, C1-C10-alkoxy-, C1-C10-alkanoyloxy-, benzyloxy-, C1-C10-alkanoyl-, C1-C10-hydroxyalkyl- or benzoyl, where R^9a and r^9b are the same or different and the same as R.⁹ represent a hydrogen atom or a C1-C10-alkyl group as well as for the residue -NR^9aR^9b also physiologically compatible salts with acids and for the residue -CO2R⁹with r⁹ denoting a hydrogen atom, physiologically compatible salts with bases.

Preferably, St is a steroid ring system of partial formula A.

Preferably, St is a steroid ring system of partial formula B.

Preferably, St is a steroidal ring system of partial formula C.

Preferably, n = 2, 3 or 4.

Preferably, o = 0.

PL 191 808 B1

More preferably, n = 2.

₃

Preferably, R.³ represents a free hydroxyl group.

Preferably, R.⁸ stands for the remainder of Y.

Preferably, Y is a C1-C10 acyl group.

More preferably, Y is a formyl, acetyl or propionyl group.

Preferably, Y is a C1-C10-hydroxyalkyl group.

More preferably, Y is a hydroxymethyl or 1-hydroxyethyl group.

Preferably, Y is hydroxy.

Preferably, Y is acetyloxy.

Preferably, Y is a methoxycarbonyl group.

Preferably, R.⁸ represents an aryl residue substituted with a Y group.

Preferably, the aryl moiety is phenyl, naphthalenyl, furanyl, benzofuranyl, thienyl, or pyridinyl.

More preferably, R.⁸ represents a 4-cyanophenyl residue.

More preferably, R.⁸ represents a 4-halophenyl residue.

More preferably, R.⁸ represents a 4-fluorophenyl residue.

Preferably, each R.⁴ and r⁵ is hydrogen.

Preferably, R.⁴ and r⁵ together they represent an additional bond.

Preferably, each R.⁶ and r⁷ is hydrogen.

The 17-α fluoroalkyl steroids of the invention are:

e- (4-acetylphenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one; 4 '- [17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) estr-4-en-11-yl] [1,1'-biphenyl] -4-carbonitrile ;

1- (4'-fluoro [1,1'-biphenyl] -4-yl) -17e-hydroxy-17- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one; 17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -11e- [4- (3-pyridinyl) phenyl] ester-4-en-3-one;

11β - (4-acetylphenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,15-dien-3-one; 4 '- [17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,15-diene-1-yl] [1,1'-biphenyl] -4- carbonitrile;

1ie- (4'-fluoro [1,1'-biphenyl] -4-yl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,15-dien-3- he;

17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -1ie- [4- (3-pyridinyl) phenyl] estra-4,15-dien-3-one; 11 e- (4-acetylphenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one; 4 '- [17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) -estra-4,9-dien-11-yl] [1,1'-bipheny10] - 4-carbonitrile;

11e- (4'-fluoro [1,1'-biphenyl] -4-yl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3- he;

17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -11e- [3-pyridinyl) estra-4,9-dien-3-one;

11e- (4-acetylphenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9,15-trien-3-one;

4 '- [17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9,15-trien-11e-yl] [1,1'-biphenyl] - 4-carbonitrile;

11e- (4'-fluoro [1,1'-biphenyl] -4-yl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl)) estra-4,9,15-triene -3-on;

17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -11e- [4- (3-pyridinyl) phenyl] estra-4,9,15-trien-3-one; 6'-acetyl-9,11a-dihydro-17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10,

9.11] ester-4-en-3-one;

4- [9,11a-dihydro-17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10, 9,

11] ester-4-en-6'-yl] benzonitrile;

9,11a-dihydro-6- (4-fluorophenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl- [3 ', 2', 1 ': 10,

9.11] ester-4-en-3-one;

9,11a-dihydro-17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -6 '- (3-pyridinyl) -4'H-naphthyl- [3', 2 ', 1' : 10,9,11] ester-4-en-3-one;

6'-acetyl-9,11a-dihydro-17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10,

9.11] ester-4,15-dien-3-one;

4- [9,11a-dihydro-17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10,

9.11] estra-4,15-diene-6'-yl] benzonitrile;

9,11a-dihydro-6 '- (4-fluorophenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3', 2 ',

1 ': 10,9,11] estra-4,15-dien-3-one;

PL 191 808B1

9,11a-dihydro-17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -6 '- (3-pyridinyl) -4'H-naphthyl [3', 2 ',

1 ': 10,9,11] estra-4,15-dien-3-one;

17e-hydroxy-11 e- (4-hydroxyphenyl) -17- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one;

17e-hydroxy-11 e- (4-hydroxyphenyl) -17- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one;

9,11a-dihydro-6 ', 17e-dihydroxy-17a (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3', 2 ', 1': 10,9,11] - ester-4-en-3-one;

1- [4- (acetyloxy) phenyl] -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one;

e- [4- (acetyloxy) phenyl] -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one;

6 '- (acetyloxy) -9,11a-dihydro-17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3', 2 ',

1 ': 10,9,11] -estr-4-en-3-one;

17e-hydroxy-11 e- [4- (hydroxymethyl) phenyl] -17- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one;

17e-hydroxy-11 e- [4- (hydroxymethyl) phenyl] -17- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one;

9,11a-dihydro-17e-hydroxy-6 '- (hydroxymethyl) -17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3', 2 ',

1 ': 10,9,11] -estr-4-en-3-one;

4- [17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9-diene-11-yl] benzaldehyde;

4- [17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) estr-4-en-11-yl] benzaldehyde;

9,11a-dihydro-17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10.9.11 ] ester-4-en-6'-al;

4- [17e-hydroxy-3-oxo-17- (1,1,2,2,2-pentafluoroethyl) estra-4,9-diene-11-yl] benzoic acid methyl ester;

4- [17e-Hydroxy-3-oxo-17a (1,1,2,2,2-pentafluoroethyl) ester-4-en-11-β-yl] -benzoic acid methyl ester;

9,11a-dihydro-17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl- [3 ', 2', 1 'acid methyl ester: 10 , 9,11] ester-4-en-6'-carboxylic;

17e-hydroxy-11e- [4- (1-hydroxyethyl) phenyl] -17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one;

17e-hydroxy-11 β- [4- (1-hydroxyethyl) phenyl] -17a- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one;

9,11a-dihydro-17e-hydroxy-6 '- (1-hydroxyethyl) -17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl- [3', 2 ', 1' : 10,9,11] -ester-4-en-3-one.

The pharmaceutical preparations according to the invention contain at least one 17a-lauoroalkyl steroid of the general formula I and a pharmaceutically acceptable carrier.

Preferably, the pharmaceutical preparations additionally contain at least one compound with anti-estrogenic activity.

The 17-α fluoroalkyl steroids of the general formula I according to the invention are characterized in that they can be used as medicaments.

The 17-α fluoroalkyl steroids are preferably suitable for use as medicaments together with a compound having an anti-estrogenic effect.

Wavy line for the rest of R.⁶ and r⁷ means that the specified substituent may be in the α- or β- position.

Within the framework of the present invention, said alkyl groups R.⁶ and r⁷ relate to methyl, ethyl, n-, iso- or tert-butyl groups. In the other C1-C10 alkyl groups of Y, R⁹, R^9a, R^9b they additionally include higher homologues such as, for example, pentyl, neo-pentyl, hexyl to decyl groups. By the term C1-C10 it is meant carbocyclic or alkyl-cycloalkyl groups with up to 10 carbon atoms, such as, for example, cyclopropyl, cyclopentyl, cycloheptyl, methylcyclopropyl, methylcyclopentyl or methylcyclohexyl residues.

In the above cases, a methyl or ethyl group is preferable.

The C1-C10 alkoxy groups represent oxygen extended moieties derived from the above-mentioned alkyl groups, i.e., for example, methoxy, ethoxy, n- or iso-propoxy, n-, iso- or tert-butyl residues.

C1-C10 alkanoyl means acyl residues of straight-chain and branched C1-C10 alkylcarboxylic acids, such as, for example, formyl, acetyl, propionyl, butyryl, or isobutyryl, etc.

Alkanoyloxy C1-C10 residues represent the above oxygen extended alkanoyl residues, such as, for example, acetyloxy, propionyloxy, butyryloxy residues.

When halogen is mentioned as a substituent, it may be fluoro, chloro or bromo. Fluorine is preferred.

PL 191 808 B1 ₂

As the rest of R.² perfluorinated side chains of length n = 2-4 are preferred, especially the pentafluoroethyl unit.

R³ essentially represents a free hydroxyl group.

In the case of an etherified or esterified hydroxyl group as the 17e substituent, it is preferably etherified with a C1-C10 alkyl group or esterified with a C1-C10 alkanoyl group. The definition of the alkyl or alkanoyl groups is the same as above. Etherification or esterification of the hydroxyl group is carried out according to methods commonly known in the art.

R⁴ and r⁵ each is hydrogen or, collectively, an additional bond.

If R⁸ represents the group Y, it is preferably a C1-C10 alkanoyl or (1-hydroxy) -C1-C10-alkyl group, of which acetyl and propionyl are preferred.

Preferred carbocyclic or heterocyclic aryls are phenyl, 1- or 2-naphthalinyl, 2- or 3-furanyl, 2- or 3-benzofuranyl, 2- or 3-thienyl, 2-, 3- or 4-pyridinyl. As substituted aryl residues, R.⁸ the 4-cyanophenyl residue and the 4-halophenyl residue, in particular the 4-fluorophenyl residue, should be mentioned in the first instance.

Of all, R.⁸ an especially preferred residue is R.⁸ as Y, and as Y, acetyl is again preferred.

The formation of the fluorinated side chain at position 17α is carried out analogously to the processes described for many other side chains by the nucleophilic addition of an organometallic compound of formula MCnFmHo to the 17-ketone of general formula II.

<img file="PL191808B1_D0003.tif" />

wherein M is a metal e.g. Li, Na, K, Mg-halogen (halogen = Cl, Br, I) or other metal, and n, m and o are as defined for formula I. Addition of Grignard reagents (CnFmHoMg-halogen) is preferred or organolithium compounds of the LiCnFmHo type. For the introduction of perfluorinated side chains it is particularly suitable to generate organolithium reactants starting from the corresponding iodides with the aid of a methyllithium / lithium bromide complex (J. Org. Chem. 1987, 52, 2481 and Tetrahedron Lett. 1985, 26, 5243).

145

In general formula II, the listed R substituents¹, R⁴, R⁵ and St are as defined in general formula I, optionally, the functional groups for St may be protected according to methods known in the art. Especially carbonyl groups, such as, for example, the 3-keto group, are in most cases protected in an appropriate manner, for example, by formation of the corresponding ketal or reduction to a hydroxyl group and optionally converting this group to an ether or ester.

As ketal protecting groups, for example, ethylenedioxy or 2,2-dimethylpropylene-1,3-dioxy can be mentioned. Other commonly known keto protecting groups are also suitable. When protecting a hydroxyl group, it may be protected as methoxymethyl, methoxyethyl, tetrahydropyranyl or silyl ethers. By cleavage of the protecting group and oxidation of the free hydroxyl group, a carbonyl group is obtained.

At the appropriate stage after the addition of the 17α side chain, the protecting groups are removed in a known manner and, if necessary, the hydroxyl group is oxidized to the corresponding keto group.

The 17α side chain addition can also be performed in the presence of other carbonyl groups, e.g. by selective addition to the 3-keto group.

The compounds of general formula II used as starting materials for the preparation of compounds of general formula I have been described in several patents, patent applications and publications: EP-A 0 057 115, EP-A 0 129 499, EP-A 0 259 2489, EP-A 0 057 115, EP-A 0 259 2489, EP-A 0 057 115 A 0 186 834, EP-A 0447 014, EP-A0 116 974, EP-A 0 190 759, EP-A 0147 361, EP-A 192 598, EP-A 0283 428, EP-A 0404 283,

PL 191 808B1

WO-A 89/00578, WO-A 91/18917, WO-A 91/18918, WO-A 92/11277, WO-A 92/11278, WO-A 93/23020, Steroids 44 (1984), 349 as also in other publications known to those skilled in the art.

The above documents also disclose the introduction of R residues⁴, R⁵, R⁶, R⁷ and r⁸which follows analogously to the residues of the present invention.

In general, the addition of the side chain to the free keto 17α group may occur at any intermediate stage of the synthesis.

In the case where the introduction of the fluorinated 17α alkyl side chain is carried out at an earlier intermediate stage of the synthesis, the introduction of additional radicals R⁶, R⁷and r⁸ for St can be performed in the presence of a 17α side chain according to known methods as disclosed in the above-mentioned patents, patent applications and publications.

The new compounds of the general formula I are valuable active pharmaceutical ingredients. They are distinguished by a very strong anti-gestagenic effectiveness. They are competitive progesterone antagonists that displace progesterone from its receptor. At the same time, other endocrine side effects, such as androgenic, estrogenic or anti-glucocorticoid activity, if at all, are only marginally present. Therefore, these compounds can be used for medical purposes.

Compounds with antigestagenic efficacy (competing progesterone antagonists) were first known in 1982 (RU 486 = EP-A 0 057 115) and have been frequently described since then, incl. in the already mentioned patents and references. Among the compounds disclosed so far, none with a multi-fluorinated 17α alkyl side chain containing at least 2 carbon atoms is present. Only WO83 / 03099 discloses that 3-keto-D^4,9The -19-norsteroids may have a 17α alkyl side chain which may optionally be substituted with halogen. Fluorine as a halogen was not listed. Previously, there were no specific examples with a 17α alkyl chain having at least 2 carbon atoms.

Active substances of this type with strong antigestagenic activity are suitable for inducing a miscarriage because they displace progesterone from the receptor, which is needed to support pregnancy. Therefore, they are valuable and interesting for their use in post-coital fertility control.

The compounds of the general formula I according to the invention are also suitable for the preparation of preparations for contraception for women (WO-A 93/23020, WO-A 93/21927).

They can also be used against hormonal imbalances as agents to induce menstruation and initiate labor. Other areas of indications in the field of gynecology are hormone compensation therapy (WO-A 94/18983), treatment of symptoms associated with menstrual disorders and endometriosis (EP-A 0 266 303) as well as myomas.

The compounds according to the invention show potent activity against neoplastic tumors and progesterone receptor positive forms of breast cancer in humans and rodents. The antiproliferative activity has been observed in vitro on human T47D breast cancer cells. In vivo, tumor growth inhibition effects have been demonstrated on MXT-neoplastic tumor of the mouse mammary gland and on forms of breast cancer in rats induced chemically by NMU (N-nitrosomethylurea) or DMBA (dimethylbenzanthracene).

The compounds according to the invention are highly useful for the treatment of hormone-dependent cancers, such as, for example, progesterone receptor positive breast cancer. The compounds according to the invention can be considered in the treatment of hormone-dependent cancer in both "first-line" and "second-line" therapy, in particular when treatment with Tamoxifen has failed.

The antigestagenic compounds of the general formula I according to the invention can also be used together with anti-estrogen-active compounds for the preparation of pharmaceutical preparations for the treatment of hormone-dependent neoplastic tumors (EP-A 0 310 542), for inducing labor, for termination of pregnancy, and also for termination of pregnancy. for the treatment of gynecological disorders (EP-A 0 310 541) and in female contraception (WO 96/19997).

In the treatment of hormone dependent neoplastic tumors, simultaneous or sequential administration of antigestagens and antiestrogens can be used. When used sequentially, usually the antiestrogen is used first, followed by the antigestagen.

The compositions of antigestagens and antiestrogens according to the invention include, for example: Tamoxifen, ICI 182.780 (= 7a- [9- (4,4,5,5,5-pentafluoropentylsulfnyl) -nonyl] ester 1,3,5 (10) -triene-3 , 17e-diol), anti-estrogen compounds described in PCT / EP97 / 04517 and aromatase inhibitors, for example Fadrozole, Formestan, Letrozole, Anastrozole or Atamestan.

PL 191 808 B1

The following were investigated as antigestagens:

A: 11β- (4-acetylphenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one (Example 1);

B: 11 e- (4-acetylphenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one (Example 3);

C: 6-acetyl-9,11a-dihydro-17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10,

9.11] ester-4-en-3-one (Example 5);

D: 11e- [4- (dimethylamino) phenyl] -17e-hydroxy-17a- (1-propionyl) estra-4,9-diene-3-one (RU 38 486);

E: 11e- (4-acetylphenyl) -19,24-dinor-17,23-epoxy-17a-chola-4,9,20-trien-3-one (Org 33 628).

All of the following tests were carried out on rats according to known methods.

Subcutaneous and oral abortion test: see for example EP-A 0 283 428

Oral androgen test: stimulation of prostate weight with test compound, vehicle: subcutaneously benzyl benzoate / castor oil (1 + 4); orally NaCl-Myrj; testosterone propionate comparison compound. Up to a dose of 10 mg test compound / animal / day, virtually no stimulation of prostate weight was observed.

Oral estrogen uterine growth test: stimulation of uterine weight with test compound, vehicle: subcutaneously benzyl benzoate / castor oil (1 + 4); orally NaCl-Myrj; three-day treatment of ovariectomized animals; parameter: uterine weight and endometrial epithelial thickness; vaginal swab negative; reference compound: estradiol 0.1 μg.

Oral antithymolysin test for estrogen effects: see for example EP-A 0 283 428.

<td>Test number</td><td>Relationship A</td><td>Relationship B</td><td>Relationship C</td><td>Relationship D</td><td>Union E</td>

<td> 1</td><td> 0,3 (4/4)</td><td> 0,3 (4/4)</td><td> 0,3 (4/4)</td><td> 3 (4/4)</td><td> 0,3 (4/4)</td>

<td></td><td> 0,1 (4/4)</td><td> 0,1 (4/4)</td><td> 0,1 (4/4)</td><td> 1 (3/4)</td><td> 0,1 (3/4)</td>

<td></td><td> 0,03 (4/4)</td><td> 0,03 (4/4)</td><td> 0,03 (4/4)</td><td> 0,3 (0/6)</td><td> 0,03 (0/4)</td>

<td> 2</td><td> 0,3 (4/4)</td><td> 0,3 (4/4)</td><td> 0,3 (4/4)</td><td> 3 (4/4)</td><td> 0,3 (4/4)</td>

<td></td><td> 0,1 (4/4)</td><td> 0,1 (4/4)</td><td> 0,1 (4/4)</td><td> 1 (2/4)</td><td> 0,1 (4/4)</td>

<td></td><td> 0,03 (4/4)</td><td> 0,03 (4/4)</td><td> 0,03 (4/4) 0,01 (4/4) 0,003 (4/4)</td><td> 0,3 (0/4)</td><td> 0,03 (0/4)</td>

<td> 3</td><td> 3 (3,3)</td><td> 3 (0)</td><td></td><td> 10 (7,2)</td><td> 10 (4,4)</td>

<td></td><td> 1 (4,8)</td><td> 1 (2,1)</td><td>undefined</td><td> 3 (2,9) 1 (1,6)</td><td> 3 (5,6) 1 (4,0)</td>

<td> 4</td><td> 10 (3,7)</td><td> 10 (2,6)</td><td> 10 (4,4)</td><td> 10 (6,4)</td><td> 10 (2,4) 3 (1,3)</td>

<td> 5</td><td> 10 (11,5)</td><td> 10 (18,2)</td><td> 22 (18,8)</td><td> 10 (76)</td><td> 10 (44,7)</td>

<td></td><td> 3 (7,4)</td><td> 3 (21,6)</td><td> 6,7 (31,6)</td><td> 3 (79)</td><td> 3 (19,1)</td>

<td></td><td> 1 (6,0)</td><td> 1 (1,2)</td><td> 2,2 (7,2)</td><td> 1 (19)</td><td> 1 (4,3)</td>

Descriptions for the table:

1: subcutaneous rat abortion test, dose [mg / animal / day], (n abortions / n total);

2: oral rat abortion test, dose [mg / animal / day], (n abortions / n total);

3: androgen test in rats, dose [mg / animal / day], (% prostate stimulation);

4: rat uterine growth test orally, dose [mg / animal / day], (% stimulation of uterine weight);

5: oral antithymolysin test, dose [mg / animal / day], (% abandonment of dexamethasone-induced thymus-induced suppression).

PL 191 808B1

The invention also relates to medicaments based on pharmaceutically compatible, i.e. at non-toxic doses, compounds of the general formula I, optionally in combination with an antiestrogen, together with the usual auxiliaries and carriers.

Further, the present invention also relates to the use of compounds of general formula I, optionally in combination with an antiestrogen, for the production of medicaments.

The compounds according to the invention can be processed according to known galenic methods into pharmaceutical preparations for enteral, transdermal, parenteral and topical use. They can be administered in the form of tablets, dragees, gel capsules, granules, suppositories, implants, sterile injectable aqueous or oily solutions, suspensions or emulsions, ointments, creams and gels, or intravaginally (e.g. vaginal pessaries) or for intrauterine use ( pessaries, spirals).

The active substance or substances can be mixed with common galenical auxiliaries, such as gum arabic, talc, starch, mannitol, methylcellulose, lactose, surfactants such as Tween or Myrj, magnesium stearate, aqueous or non-aqueous vehicles, paraffin derivatives, wetting agents , dispersing, emulsifying, preserving, and flavoring and improving the taste (e.g. essential oils).

A unit dose contains approximately 0.1-100 mg of active ingredient (s).

The dosage of the compounds of the invention in humans is about 0.1-400 mg per day.

Example 1 e- (4-acetylphenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one Ia) 3.3; 17.17 -bis [1,2-ethanediylbis (oxy)] - 11 e- [4 - [[(1,1,2,2,3,3,4,4,4-nonafluorobutyl) sulfonyl] oxy] phenyl] ester -5-en

To a solution of 6 g of 4- [3.3; 17,17-bis [ethanediylbis (oxy)] ester-5-en-1-yl] phenol, the preparation of which is described in WO 91/18917 and WO 91/18918, in 100 ml absolute tetrahydrofuran was added at 0 ° C to 9.2 ml of a 1.6 molar solution of butyllithium in hexane. The mixture was stirred for 30 minutes at 0 ° C and then 5 ml of 1,1,2,2,3,3,4,4,4-nonafluoro-1-butanesulfonyl fluoride was added. The mixture was stirred for one hour at 0 ° C. The reaction mixture was then poured into a saturated aqueous sodium bicarbonate solution. Extracted with ethyl acetate, washed with saturated aqueous sodium chloride solution, dried over sodium sulfate and concentrated in vacuo. After column chromatography of the crude product on silica gel with a hexane / ethyl acetate mixture, 8.2 g of 1a) were obtained as white foam.

¹H NMR (CDCl3): δ = 7.45 d (J = 9 Hz, 2H, aryl); 7.17 d (J = 9 Hz, 2H, aryl); 5.55 dbr (J = 5 Hz, 1H, H-6); 4.00-3.80 m (8H, ketals); 3.50 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 0.53 s (3H, H-18).

1b) 3,3- [1,2-ethanediylbis (oxy)] - 11 e- [4 - [[(1,1,2,2,3,3,4,4,4-nonafluorobutyl) sulfonyl] oxy] -phenyl] -ester-5-en-17-one

8.2 g of the compound described in 1a) were stirred for 5 hours at room temperature with 22 g of silica gel and 2 ml of a saturated aqueous solution of oxalic acid in 85 ml of dichloromethane. The mixture was then filtered through Celite®. It is concentrated by evaporation in vacuo and the crude product is purified by crystallization from diisopropyl ether. 5.3 g of 1b) are obtained in the form of white crystals.

¹H NMR (CDCl3): δ = 7.45 d (J = 9 Hz, 2H, aryl); 7.19 d (J = 9 Hz, 2H, aryl); 5.59 dbr (J = 5 Hz, 1H, H-6); 4.00-3.88 m (4H, ketal); 3.52 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 0.55 s (3H, H-18).

1c) 3,3- [1,2-ethanediylbis (okxy) -11 β- [4 - [[(1,1,2,2,3,3,4,4,4-nonafluorobutyl) sulfonyl] oxy] - phenyl] -17α- (1,1,2,2,2-pentafluoroethyl) estr-5-en-17β-ol ml of condensed pentafluoroethyl iodide was mixed with a solution of 691 mg 1b) in 10 ml of absolute diethyl ether at -78 ° C. At this temperature, 4.77 ml of a 1.5 molar solution of methyl lithium-lithium bromide complex in diethyl ether were added. The mixture was then stirred for one hour at -78 ° C. The reaction mixture was then poured into a saturated aqueous sodium bicarbonate solution. Extracted with ethyl acetate, washed with saturated aqueous sodium chloride solution, dried over sodium sulfate and concentrated in vacuo. Chromatography of the crude product obtained with a hexane / ethyl acetate mixture on silica gel gave 719 mg of 1c) as white foam.

¹H NMR (CDCl3): δ = 7.45 d (J = 9 Hz, 2H, aryl); 7.19 d (J = 9 Hz, 2H, aryl); 5.54 dbr (J = 5 Hz, 1H, H-6); 4.88-4.00 m (4H, ketal); 3.53 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 0.60 s (3H, H-18).

PL 191 808 B1 ld) 11β- (4-acetylphenyl) -3,3- [1,2-ethanediylbis (oxy)] - 17α- (1,1,2,2,2-pentafluoroethyl) ester-5-en- 17β-ol

A solution consisting of 719 mg 1c), 0.45 ml of (1-ethoxyethenyl) tributyltin hydride, 41 mg of tetrakis- (triphenylphosphine) palladium (0), 263 mg of lithium chloride and 0.1 ml of pyridine in 12 ml of dioxane was heated for 1 , 5 hours under reflux. The reaction mixture was then poured into water. It was extracted with ethyl acetate and a saturated aqueous ammonium chloride solution as well as 3 ml of a saturated aqueous oxalic acid solution were added to the organic phase. Stirred for 30 minutes at room temperature. The organic phase was then separated and washed with an aqueous saturated sodium bicarbonate solution and sodium chloride. Dried over sodium sulfate and concentrated in vacuo. After column chromatography of the crude product obtained on silica gel with a hexane / ethyl acetate mixture, 440 mg of 1d) was obtained.

¹H NMR (CDCl3): δ = 7.88 d (J = 9 Hz, 2H, aryl); 7.47 d (J = 9 Hz, 2H, aryl); 5.55 dbr (J = 5 Hz, 1H, H-6); 4.88-4.00 m (4H, ketal); 3.53 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 2.61 s (3H, acetyl); 0.62 s (3H, H-18).

le) 11 β- (4-acetylphenyl) -17β-hydroxy-17α- (1,1,2,2,2-pentafluoroethyl) ester-4-en-3-one

440 mg 1d) was dissolved in 10 ml of acetone. 1 ml of 4N hydrochloric acid was added and stirred 1.5 hours at room temperature. The reaction mixture was then poured into a saturated sodium bicarbonate solution. It is extracted with dichloromethane, the organic phase is washed with a saturated aqueous sodium chloride solution, dried over sodium sulphate and concentrated in vacuo. Column chromatography of the crude product obtained on silica gel with a hexane / ethyl acetate mixture gave 311 mg1e) in the form of a white foam.

¹H NMR (CDCl3): δ = 7.89 d (J = 9 Hz, 2H, aryl); 7.53 d (J = 9 Hz, 2H, aryl); 5.89 sbr (1H, H-4);

3.50 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 2.84 m (1H, H-10); 2.60 s (3H, acetyl); 0.70 s (3H, H-18).

Example 2 β- (4-acetylphenyl) -17β-hydroxy-17α- (1,1,2,2,3,3,4,4,4-nonafluorobutyl) ester-4-en-3-one

2a) 3,3- [1,2-ethanediylbis (oxy)] - 17Q- (1,1,2,2,3,3,4,4,4-nonafluorobutyio) -11 β- [4 - [[1 , 1,2,2,

3,3,4,4,4-nonafluorobutyl) sulfonyl] oxy] phenyl] estr-5-en-17β-ol

Analogously to example 1c) 691 mg 1b) in 15 ml of absolute diethyl ether was mixed with the reagent formed from 0.52 ml of 1-iodo-1,1,2,2,3,3,4,4,4-nonafluorobutane and 1. 67 ml of a 1.5 molar solution of methyl lithium-lithium bromide complex in diethyl ether. Chromatography on silica gel with hexane / ethyl acetate gave 838 mg of 2a) as white foam.

¹H NMR (CDCl3): δ = 7.44 d (J = 9 Hz, 2H, aryl); 7.18 d (J = 9 Hz, 2H, aryl); 5.55 dbr (J = 5 Hz, 1H, H-6); 3.88-4.00 m (4H, ketal); 3.53 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 0.61 s (3H, H-18).

2b) 11 β- (4-acetylphenyl) -3,3- [1,2-ethanediylbis (oxy)] - 17α- (1,1,2,2,3,3,4,4,4-nonafluoro-butyl ) ester-5-en-17β-ol

Analogously to Example 1d) 838 mg of 2a) were reacted with 0.46 ml of (1-ethoxyethenyl) tributyltin hydride, 43 mg of tetrakis (triphenylphosphine) palladium (0), 272 mg of lithium chloride and 0.1 ml of pyridine in 12 ml of dioxane. After the reaction and treatment with a saturated aqueous ammonium chloride solution as well as a saturated aqueous solution of oxalic acid and column chromatography on silica gel with a hexane / ethyl acetate mixture, 505 mg of 2b) were obtained as white foam.

¹H NMR (CDCl3): δ = 7.85 d (J = 9 Hz, 2H, aryl); 7.46 d (J = 9 Hz, 2H, aryl); 5.55 dbr (J = 5 Hz, 1H, H-6); 3.88-4.00 m (4H, ketal); 3.53 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 2.61 s (3H, acetyl); 0.63 s (3H, H-18).

2c) 11 β- (4-acetylphenyl) -17β-hydroxy-17α- (1,1,2,2,3,3,4,4,4-nonafluorobutyl) -ester-4-en-3-one

Analogously to example 1e) 505 mg of 2b) were mixed with 4N hydrochloric acid in acetone. Column chromatography on silica gel with hexane / ethyl acetate gave 372 mg of 2c) as a white foam.

¹H NMR (CDCl3): δ = 7.89 d (J = 9 Hz, 2H, aryl); 7.55 d (J = 9 Hz, 2H, aryl); 5.88 sbr (1H, H-4);

3.51 ddbr (J = 7 Hz + 5 Hz, 1H, H-11); 2.85 m (1H, H-10); 2.60 s (3H, acetyl); 0.70 s (3H, H-18).

PL 191 808B1

Example 3 β- (4-acetylphenyl) -17β-hydroxy-17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

3a) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 17α- (1,1,2,2,2-pentafluoroethyl) -11β- [4- (2,5,

5-trimethyl-1,3-dioxolan-2-yl) phenyl] -5α-ester-9-en-5,17β-diol

Analogously to example 1c) 1.08 g of 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 5-hydroxy-11 β- [4- (2,5,5-trimethyl- 1,3-dioxolan-2-yl) phenyl] -5α-ester-9-en-17-one, the preparation of which is described in EP 0190759, example 6c), in 19 ml absolute diethyl ether with 1.9 ml 1-iodo1 , 1,2,2,2-pentafluoroethane and 8.7 ml of a 1.5 molar solution of methyl lithium-lithium bromide complex in diethyl ether. Chromatography on silica gel with hexane / ethyl acetate gave 644 mg of the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.29 d (J = 9 Hz, 2H, aryl); 7.23 d (J = 9 Hz, 2H, aryl); 4.42 s (1H, 5-OH); 4.35 dbr (J = 7 Hz + 5 Hz, 1H, H-11); 1.52 s (3H, arylketal); 1.26 s (3H, arylketal); 1.04 s (3H, 3-ketal); 0.89 s (3H, 3-ketal); 0.57 s (3H, arylketal); 0.51 s (3H, H-18).

3b) 11 β- (4-acetylphenyl) -17β-hydroxy-17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

635 mg of the compound described in 3a) in 9 ml of methanol with 0.4 ml of aqueous semi-concentrated sulfuric acid was stirred for two hours at room temperature. The mixture was then poured into a saturated aqueous sodium hydrogen carbonate solution and extracted with ethyl acetate. The organic phase was washed with a saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered and concentrated in vacuo. Column chromatography on silica gel with hexane / ethyl acetate gave 428 mg of the title compound as a colorless foam.

Temp. melting point: 260.4 ° C (diisopropyl ether), [α] '² = + 181.3 ° (CHCl₃, c = 0.535).

Example 4

11β- (4-acetylphenyl) -17β-hydroxy-17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9,15-trien-3-one

4a) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 17α- (1,1,2,2,2-pentafluoroethyl) -11 β- [4- (2,5,

5-trimethyl-1,3-dioxolan-2-yl) phenyl] -5α-estra-9,15-dien-5,17β-diol

Analogously to example 1c) 1.15 g 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 5-hydroxy11 β- [4- (2,5,5-trimethyl-1,3 -dioxolan-2-yl) phenyl] -5α-estra-9,15-diene-17-one, the preparation of which is described in WO 89/00578, example 1b), was reacted in 20 ml of absolute diethyl ether and 10 ml of absolute tetrahydrofuran with a reagent formed from 2.0 mL of 1-iodo-1,1,2,2,2-pentafluoroethane and 9.3 mL of a 1.5 molar solution of methyl lithium-lithium bromide complex in diethyl ether. Chromatography on silica gel with hexane / ethyl acetate gave 1.12 g of the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.31 d (J = 9 Hz, 2H, aryl); 7.24 d (J = 9 Hz, 2H, aryl); 6.31 dbr (J = 6 Hz, 1H, H-15); 5.58 dddbr (J = 6 Hz + 3.5 Hz + 1.5 Hz, 1H, H-16); 4.49 s (1H, 5-OH); 4.40 dbr (J = 8 Hz + 5 Hz, 1H, H-11); 1.52 s (3H, arylketal); 1.26 s (3H, arylketal); 1.03 s (3H, 3-ketal); 0.89 s (3H, 3-ketal); 0.68 s (3H, H-18); 0.58 s (3H, arylketal).

4b) 11β- (4-acetylphenyl) -17β-hydroxy-17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9,15-trien-3-one

1.15 g of the compound described in 4a) in analogy to the method described in 3b) were reacted in 16.5 ml of methanol with 0.73 ml of aqueous semi-concentrated sulfuric acid to obtain 572 mg of the title compound in the form of a colorless foam.

Temp. melting point: 213.9 ° C (diisopropyl ether), [α] '² + 210.5 ° (CHCl₃, c = 0.615).

Example 5

9,11α-dihydro-6 '- (4-fluorophenyl) -17β-hydroxy-17α- (1,1,2,2,2-pentafluoroethyl) -4Ή-naphthyl [3', 2 ',

1 ': 10.9,11] ester-4-en-3-one

5a) 9,11α-dihydro-3,3, [2,2-dimethyl-1,3-propanediylbis (oxy)] - 6 '- [[(1,1,2,2,3,3,4,4 , 4-nona-fluorobutyl) sulfonyl] oxy] -17α- (1,1,2,2,2-pentafluoroethyl) -4Ή-naphthyl [3 ', 2', 1 ': 10,

9,11] -5α-estran-5,17α-diol

A solution of ca. 4 ml of pentafluoroethyl iodide in 20 ml of anhydrous ether was stirred at -78 ° C under dry argon with 13.4 ml of a 1.7 molar solution of tert-butyl lithium in hexane for 30 minutes. A solution of 2.0 g (2.62 mmol) of 9.11α-dihydro-3.3, - [2,2-dimethyl-1,3-propane-diylbis (oxy)] - 5-hydroxy-6 '- [[ (1,1,2,2,3,3,4,4,4-nonafluorobutyl) sulfonyl] oxy] -4'Hnaphthyl [3 ', 2', 1 ': 10.9,11] -5α-estran- 17-one, prepared analogously to the method described in DE 4216003 (example 1b), in 60 ml of anhydrous toluene and the solution was allowed to heat to 10 ° C within 2 hours. The mixture was poured into a saturated aqueous solution of sodium bicarbonate, extracted with ethyl acetate, the combined organic extracts were washed with saturated aqueous

With sodium chloride solution and dried over sodium sulfate. After filtration, the obtained residue, after concentrating the soluble part, was purified by gradient chromatography in the system of hexane and ethyl acetate on approx. 300 ml of fine silica gel. 1.40 g (1.59 mmol, 61%) of the title compound were isolated as a colorless solid as well as 730 mg (0.96 mmol, 36%) of the starting material.

¹H NMR (CDCl3): δ = 0.41 (3H), 0.93 (3H), 1.00 (3H), 1.20-1.36 (2H), 1.42-1.81 (11H) , 1.93 (2H), 2.07-2.28 (3H), 2.31-2.48 (1H), 2.61-2.77 (2H), 3.15 (1H), 3, 21 (1H), 3.45-3.65 (4H), 4.48 (1H), 6.98 (1H), 7.04 (1H), 7.47 (1H) ppm.

5b) 9,11a-dihydro-3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 6 '- (4-fluorophenyl) -17a- (1,1,

2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10.9,11] -5a-estran-5,17e-diol

A solution of 400 mg (453 μmol) of the compound obtained according to example 5a) was mixed with 7 ml of anhydrous toluene and 3 ml of anhydrous ethanol and successively with 43 mg of lithium chloride, 0.66 ml of 2M sodium carbonate solution, 82 mg of (4-fluorophenyl) boric acid. , 50 mg of tetrakis (triphenylphosphine) palladium (0) and heated under argon for 1.5 hours at 95 ° C. The reaction mixture was diluted with water, extracted with ethyl acetate, the pooled organic extracts were washed with a saturated aqueous sodium chloride solution and dried over sodium sulfate. After filtration, the obtained residue, after concentrating the soluble part, was purified by gradient chromatography in the system of hexane and ethyl acetate on approx. 150 ml of fine silica gel. 264 mg (389 µmol, 86%) of the title compound was isolated as a colorless solid.

¹H NMR (CDCl3): δ = 0.49 (3H), 0.93 (3H), 0.99 (3H), 1.21-2.28 (18H), 2.30-2.47 (1H) , 2.76 (2H), 3.17 (1H); 3.26 (1H), 3.47-3.66 (4H), 4.48 (1H), 7.11 (2H); 7.23 (1H), 7.33 (1H), 7.45 (1H); 7.54 (2H) ppm.

5c) 9,11a-dihydro-6 '- (4-fluorophenyl) -17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3', 2 ', 1 ': 10,9,11] ester-4-en-3-one

A solution of 260 mg (383 μmol) of the compound obtained according to example 5b) in 13 ml of acetone was mixed with 0.700 ml of a 4M aqueous hydrochloric acid solution and heated for 4 hours at 50 ° C. The mixture was poured into saturated aqueous sodium bicarbonate solution, extracted several times with dichloromethane, the pooled organic extracts were washed with saturated aqueous sodium chloride solution and dried over sodium sulfate. After filtration, the obtained residue, after concentrating the soluble part, was purified by gradient chromatography in the system of hexane and ethyl acetate on ca. 100 ml of fine silica gel. 206 mg (359 µmol, 94%) of the title compound was isolated as a colorless solid.

¹H NMR (CDCl3): δ = 0.55 (3H), 1.22 (1H), 1.33-1.50 (2H), 1.54-1.89 (5H), 1.92-2, 54 (8H), 2.66 (1H), 2.81 (1H), 2.87 (1H), 3.31 (1H), 3.43 (1H), 5.90 (1H), 7.12 (2H), 7.27 (1H), 7.37 (1H), 7.45-7.60 (3H) ppm.

Example 6

6'-acetyl-9.11 a-dihydro-17e-hydroxy-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10,

9,11] ester-4-en-3-one

Solution of 600 mg (680 μmol) of the compound obtained according to example 5a) in 7 ml of anhydrous

N, N-dimethylformamide was successively mixed under dry argon with 69 mg of lithium chloride,

0.18 ml of (1-ethoxyethenyl) tributyltin hydride, 25 mg of tetrakis (triphenylphosphine) palladium (0) and heated 1.5 hours to 110 ° C. After cooling, 10 ml of acetone, 1.5 ml of aqueous 4N hydrochloric acid were added and the reaction was carried out for 2 hours at 23 ° C and then heated for another 3 hours at 50 ° C. The mixture was poured into saturated aqueous sodium bicarbonate solution, extracted several times with dichloromethane, the pooled organic extracts were washed with saturated aqueous sodium chloride solution and dried over sodium sulfate. From the residue obtained after filtration and removal of the solvent, 206 mg of the title compound, still crude, was obtained by crystallization from dichloromethane and acetone, which was subsequently purified on 10 analytical TLC plates. A mixture of hexane and ethyl acetate was used as the mobile phase, and a mixture of dichloromethane and methanol as eluent. 160 mg (306 µmol, 45%) of the title compound was isolated as a colorless solid.

¹H NMR (CDCl3): δ = 0.47 (3H), 1.21 (1H), 1.31-1.51 (2H), 1.53-1.85 (5H), 1.98 (2H) , 2.12-2.52 (6H), 2.54 (3H), 2.64 (1H), 2.82 (1H), 2.88 (1H), 3.31 (1H), 3.42 (1H), 5.91 (1H), 7.54 (1H), 7.71 (1H), 7.77 (1H) ppm.

PL 191 808B1

Example 7

4- [9,11a-dihydro-17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10, 9,11] -ester-4-en-6'-yl] benzonitrile

7a) 4- [9,11a-dihydro-5,17e-hydroxy-3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 17a- (1,1,

2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2', 1 ': 10,9,11] -5a-estran-6'-yl] benzonitrile

400 mg (453 μmol) of the compound obtained according to example 5b) was reacted analogously to example 5b) with 4- (5,5-dimethyl-1,3,2-dioxaboran-2-yl) benzonitrile, and after completion of the reaction and purification, 301 mg (439 µmol, 97%) of the title compound as a crystalline solid.

7b) 4- [9,11a-dihydro-17e-hydroxy-3-oxo-17a- (1,1,2,2,2-pentafluoroethyl) -4'H-naphthyl [3 ', 2',

1 ': 10,9,11] ester-4-en-6'-yl] benzonitrile

296 mg (431 µmol) of the compound obtained in Example 7a) were reacted analogously to Example 5c) and, after completion of the reaction and purification, 228 mg (392 µmol, 91%) of the title compound were isolated as a crystalline solid.

¹H NMR (CDCl3): δ = 0.53 (3H), 1.22 (1H), 1.35-1.51 (2H), 1.55-1.88 (5H), 1.92-2, 14 (3H), 2.14-2.53 (5H), 2.65 (1H), 2.81 (1H), 2.88 (1H), 3.32 (1H), 3.45 (1H) , 5.91 (1H), 7.32 (1H), 7.42 (1H), 7.55 (1H), 7.70 (4H) ppm.

Example 8

17e-hydroxy-11 e- (4-hydroxyphenyl) -17a- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

8a) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 5-hydroxy-11 e- [4- (phenylmethoxy) phenyl] -5a-ester-9-en-17-one

1.17 g of magnesium turnings are placed in 4 ml of absolute tetrahydrofuran under a protective gas and mixed with a drop of 1,2-dibromomethane. After the initiation of the reaction, a solution of 12.7 g of 1-bromo-4- (phenylmethoxy) benzene (for preparation see J. Amer. Chem. Soc. 42, 657 (1920)) in 80 ml of absolute tetrahydrofuran was slowly added dropwise. The reaction mixture was heated to reflux until the magnesium was completely converted. After cooling to 0 ° C, the mixture was then mixed with 2.39 g of copper (I) chloride. A solution of 3 g of 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] -5,10-epoxy-5a, 10a-ester-9 (11) -en-17-one was slowly added dropwise (preparation of see Tetrahedron Lett. 26, 2069-2072 (1985)) in 80 ml of absolute tetrahydrofuran. The reaction mixture was stirred at room temperature overnight and then poured into a saturated aqueous ammonium chloride solution. The organic phase was extracted with ethyl acetate, the organic phases were combined, washed with a saturated aqueous sodium chloride solution and dried over sodium sulfate. The mixture was filtered and concentrated in vacuo. Column chromatography on silica gel with hexane / ethyl acetate gave the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.50-7.27 m (5H, benzyl); 7.13 d (J = 9 Hz, 2H, aryl); 6.88 d (J = 9 Hz, 2H, aryl); 5.02 s (2H, benzyl); 4.45 s (1H, 5-OH); 4.27 dbr (J = 6.5 Hz, 1H, H-11); 1.06 s (3H, 3-ketal); 0.87 s (3H, 3-ketal); 0.50 s (3H, H-18).

8b) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 17a- (1,1,2,2,2-pentafluoroethyl) -11 β- [4- (phenylmethoxy) phenyl] -5a-ester-9-ene-5,17e-diol

Analogously to example 1c) 1.35 g of the compound described in 8a) were mixed in 48 ml of absolute toluene with 1.18 g of 1-iodo-1,1,2,2,2-pentafluoroethane and 2.4 ml of a 1.5 molar solution of methyllithium complex. -lithium bromide in diethyl ether. Column chromatography on silica gel with hexane / ethyl acetate gave 730 mg of the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.50-7.30 m (5H, benzyl); 7.12 d (J = 9 Hz, 2H, aryl); 6.88 d (J = 9 Hz, 2H, aryl); 5.02 s (2H, benzyl); 4.45 s (1H, 5-OH); 4.29 dbr (J = 6 Hz, 1H, H-11); 1.06 s (3H, 3-ketal); 0.87 s (3H, 3-ketal); 0.56 s (3H, H-18).

8c) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 17a- (1,1,2,2,2-pentafluoroethyl) -11 β- (4-hydroxyphenyl) -5a- ester-9-ene-5,17e-diol

730 mg of the compound obtained in 8b) was dissolved in 11 ml of methanol and mixed with 341 mg of ammonium formate, 73 mg of 10% palladium on activated carbon. The reaction mixture was stirred for two hours at room temperature and then filtered through Celite^®. The residue was washed thoroughly with ethyl acetate. The filtrate was concentrated in vacuo. 631 mg of compound 8c) are obtained, which is further reacted crude.

PL 191 808 B1

8d) 17β-hydroxy-11 β- (4-hydroxyphenyl) -17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

631 mg of the compound described in 8c) were reacted in 11 ml of methanol in 11 ml of methanol with 0.48 ml of aqueous semi-concentrated sulfuric acid in an analogous manner to that described in 8c) to obtain 428 mg of the title compound as colorless foam.

¹H NMR (CDCl3): δ = 7.00 d (J = 9 Hz, 2H, aryl); 6.75 d (J = 9 Hz, 2H, aryl); 5.94 sbr (1H, OH); 5.80 s (1H, H-4); 4.38 dbr (J = 7 Hz, 1H, H-11); 0.61 s (3H, H-18).

Example 9 β- [4- (acetyloxy) phenyl] -17β-hydroxy-17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

300 mg of the compound described in 8d) was dissolved in 12 ml of pyridine and stirred for four hours with 0.061 ml of acetic anhydride. The reaction mixture was poured into a saturated aqueous ammonium chloride solution. The aqueous phase was extracted with ethyl acetate, the organic phases were combined, washed with a saturated aqueous sodium chloride solution and dried over sodium sulfate. The mixture was filtered and concentrated in vacuo. Column chromatography on silica gel with hexane / ethyl acetate gave 248 mg of the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.18 d (J = 9 Hz, 2H, aryl); 7.02 d (J = 9 Hz, 2H, aryl); 5.79 s (1H, H-4); 4.45 dbr (J = 6 Hz, 1H, H-11); 2.29 s (3H, acetyl); 0.61 s (3H, H-18).

Example 10

17β-hydroxy-11β- [4- (hydroxymethyl) phenyl] -17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

10a) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 5-hydroxy-11 β- [4 - [(methoxymethoxy) -methyl ^^ - ester ^ -en-H -he

From 6.0 g of 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 5,10-epoxy-5α, 10α-ester-9 (11) -en-17-one in 160 ml of absolute tetrahydrofuran, 22.32 g of 1-bromo-4 - [(methoxymethoxy) methyl] benzene (for preparation see Synth. Commun. 20, 1469-1472 (1990)) in 160 ml of absolute tetrahydrofuran, 2.35 g of magnesium turnings in 10 ml of absolute tetrahydrofuran and 4.78 g of copper (I) chloride in the same way as described in 8a) after column chromatography on silica gel a mixture of hexane / ethyl acetate gave 7.14 g of the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.27 d (J = 9 Hz, 2H, aryl); 7.24 d (J = 9 Hz, 2H, aryl); 4.72 s (2H, acetal); 4.56 s (2H, benzyl); 4.48 s (1H, 5-OH); 4.33 dbr (J = 6.5 Hz, 1H, H-11); 3.42 s (3H, methoxy); 1.07 s (3H, 3-ketal); 0.87 s (3H, 3-ketal); 0.49 s (3H, H-18).

10b) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 17α- (1,1,2,2,2-pentafluoroethyl) -11 β- [4 - [(methoxymethoxy) methyl ] phenyl] -5α-ester-9-ene-5,17β-diol

Analogously to example 1c) 4.85 g of the compound described in 10a) were mixed in 200 ml of absolute toluene with 18.2 g of 1-iodo-1,1,2,2,2-pentafluoroethane and 43.3 ml of a 1.5 molar solution of methyl lithium-lithium bromide complex in diethyl ether. After column chromatography on silica gel with a hexane / ethyl acetate mixture, 4.13 g of the title compound was obtained as a colorless foam.

¹H NMR (CDCl3): δ = 7.25 d (J = 9 Hz, 2H, aryl); 7.20 d (J = 9 Hz, 2H, aryl); 4.71 s (2H, acetal); 4.54 s (2H, benzyl); 4.46 s (1H, 5-OH); 4.32 dbr (J = 6 Hz, 1H, H-11); 3.41 s (3H, methoxy); 1.06 s (3H, 3-ketal); 0.86 s (3H, 3-ketal); 0.52 s (3H, H-18).

10c) Πβ-hydroxy-H β- [4- (hydroxymethyl) phenyl] -17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

4.13 g of the compound obtained in 10b) were reacted in 65 ml of methanol in 65 ml of methanol with 2.84 ml of aqueous semi-concentrated sulfuric acid in an analogous manner to that obtained in 10b) to obtain 2.26 g of the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.27 d (J = 9 Hz, 2H, aryl); 7.17 d (J = 9 Hz, 2H, aryl); 5.78 s (1H, H-4); 4.64 s (2H, benzyl); 4.64 dbr (J = 6.5 Hz, 1H, H-11); 0.59 s (3H, H-18).

Example 11

4- [17β-hydroxy-3-oxo-17α- (1,1,2,2,2-pentafluoroetulo) estra-4,9-dien-11 β-yl] enzaldehyde

497 mg of the compound obtained in 10c) was stirred for two hours at room temperature with 431 mg of pyridine chlorochromate in 10 ml of dichloromethane, and then filtered through silica gel. The residue was washed thoroughly with ethyl acetate. The filtrate was concentrated in vacuo. Column chromatography on silica gel with hexane / ethyl acetate gave the title compound as a colorless foam.

PL 191 808B1 ¹H NMR (CDCl3): δ = 9.97 s (1H, formyl); 7.81 d (J = 9 Hz, 2H, aryl); 7.39 d (J = 9 Hz, 2H, aryl); 5.81 s (1H, H-4); 4.52 dbr (J = 7 Hz, 1H, H-11); 0.58 s (3H, H-18).

Example 12

4- [17β-Hydroxy-3-oxo-17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-diene-11β-yl-enzoic acid methyl ester.

A solution of 125 mg of the compound obtained according to 11 in 2.5 ml of methanol was added to 81.4 mg of potassium cyanide in 1.25 ml of methanol. 390 mg of manganese (IV) oxide and 22 ml of acetic acid were added to the reaction mixture, followed by stirring for one hour at room temperature. The mixture was filtered_® by Celite^®, the filtrate was transferred to ethyl acetate / water and the aqueous phase was extracted with ethyl acetate. The combined organic phases were washed with water and a saturated aqueous sodium chloride solution, dried over sodium sulfate, filtered and concentrated in vacuo.

Column chromatography on silica gel with hexane / ethyl acetate gave 120 mg of the title compound as a colorless foam.

¹H NMR (CDCl3): δ = 7.94 d (J = 9 Hz, 2H, aryl); 7.27 d (J = 9 Hz, 2H, aryl); 5.79 s (1H, H-4); 4.49 dbr (J = 6 Hz, 1H, H-11); 3.89 s (3H, methoxy); 0.57 s (3H, H-18).

Example 13

17β-hydroxy-11β- [4- (1-hydroxyethyl) phenyl] -17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

13a) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 5-hydroxy-11 β- [4- [1 - [(tetrahydro-2H-pyran-2-yl) oxy] ethyl] phenyl] -5α-ester-9-en-17-one

From 1.6 g of 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 5,10-epoxy-5α, 10α-ester-9 (11) -en-17-one in 40 ml of absolute tetrahydrofuran, 7.4 g of 2- [1- (4-bromophenyl) ethoxy] tetrahydro-2H-pyran (for preparation see Arzneim. Forsch. 25, 1495-1501 (1975)) in 40 ml of absolute tetrahydrofuran, 1.3 g of magnesium turnings in 2 ml of absolute tetrahydrofuran and 1.3 g of copper (I) chloride in the same way as described in 8a) after gel column chromatography silica hexane / ethyl acetate gave 2.06 g of the title compound as a diastereomeric mixture at the acetal and benzyl positions.

¹H NMR (CDCl3): δ = 7.28 d (J = 9 Hz, 2H, aryl); 7.18 d (J = 9 Hz, 2H, aryl); 4.90-4.72 m (2H, acetal and benzyl ether); 4.44 s (1H, 5-OH); 4.30 dbr (J = 6.5 Hz, 1H, H-11); 1.45 / 1.42 d (J = 6Hz, 3H, methyl); 1.05 s (3H, 3-ketal); 0.87 s (3H, 3-ketal); 0.46 s (3H, H-18).

13b) 3,3- [2,2-dimethyl-1,3-propanediylbis (oxy)] - 17α- (1,1,2,2,2-pentafluoroethyl) -11 β- [4- [1 - [( tetrahydro-2H-pyran-2-yl) oxy] ethyl] phenyl] -5α-ester-9-en-5,17β-diol

Analogously to example 1c) 1.45 g of the compound described in 13a) were mixed in 50 ml of absolute toluene with 4.9 g of 1-iodo-1,1,2,2,2-pentafluoroethane and 11.7 ml of a 1.5 molar solution methyl lithium-lithium bromide complex in diethyl ether. After column chromatography on silica gel with a hexane / ethyl acetate mixture, 1.22 g of the title compound were obtained as a diastereomeric mixture at the acetal and benzyl positions.

¹H NMR (CDCl3): δ = 7.28 d (J = 9 Hz, 2H, aryl); 7.18 d (J = 9 Hz, 2H, aryl); 4.90-4.74 m (2H, acetal and benzyl ether); 4.42 s (1H, 5-OH); 4.31 dbr (J = 6.5 Hz, 1H, H-11); 1.46 / 1.42 d (J = 6Hz, 3H, methyl); 1.05 s (3H, 3-ketal); 0.87 s (3H, 3-ketal); 0.51 s (3H, H-18).

13c) 17β-hydroxy-11 β- [4- (1-hydroxyethyl) phenyl] -17α- (1,1,2,2,2-pentafluoroethyl) estra-4,9-dien-3-one

1.22 g of the compound obtained in 13b) was reacted in an analogous manner to that described in 3b) in 18 ml of methanol containing 0.778 ml of aqueous semi-concentrated sulfuric acid to obtain 693 mg of the title compound as a colorless foam. An epimeric mixture on the benzyl carbinol was obtained.

¹H NMR (CDCl3): δ = 7.28 d (J = 9 Hz, 2H, aryl); 7.15 d (J = 9 Hz, 2H, aryl); 5.79 s (1H, H-4); 4.88 qbr (J = 6 Hz, 1H, benzyl); 4.45 dbr (J = 6 Hz, 1H, H-11); 1.49 d (J = 6 Hz, 3H, methyl); 0.60 s (3H, H-18).