Sensor, for detecting analytes in fluids, comprising a chemically sensitive resistor electrically connected to electrical measuring apparatus

Field of the Invention

The field of the invention is electrical sensors for delecting analytes in fluids

Background _;)

There is considerable interest in developing sensors that act as analogs of the mammalian olfactory system (Lundstrdm el al (1991) Nature 352 41-50,

Shurmer and Gardner (1992) Sens Act B 8 ^^11) This system is thought to utilize probabilistic repertf ires of many different receptors to recognize a single odorant (Reed (1992) Ncuion 8 205-209, Lancet and Bcn-Aine (1993) Ctirr Biol 3 668-674) In such a configuration, the burden of recognition is not on highly specific receptors, as in the traditional lock-and-key molecular recognition approach to chemical senstng, but lies instead on the distributed pattern processing of the olfactory bulb and the brain (Kauer (1991) TINS N 79-85, DeVries and Baylor (1993) Cell 1O(S) 139-149) Poor attempts to

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-2produce a broadly responsive sensor array have exploited heated metal oxide thin film resistors (Gardner et at (1991) Sens Act B 4 117-121, Gardner et al (1991) Sens Act B 6 71-75, Corcoran et al (1993) Sens Act B 15 32-37), polymer sorption layers on the surfaces of acoustic wave resonators (Grate and

Abraham (1991) Sens Act B 3 85-111, Grate et at (1993)Anal Chem

1868-1881), arrays of electrochemical detectors (Stetter el al (1986) Anal Chem 58 860-866, Stetter et al (1990) Sens Act B 1 43-47, Stetter etal (1993) Anal Chem Acta 284 1-11), or conductive polymers (Pearce et al (1993) Analyst 118 371-377, Shurmer et al (1991) Sens Act B 4 29-33)

Arrays of metal oxide thin film resistors, typically based on SnO₂ films that have been coated with various catalysts, yield distinct, diagnostic responses for several vapors (Gardner et al (1991) Sens Act B 4 117-121, Gardner et al (1991) Sens Act B 611-15, Corcoran etal (1993) Sens Act B 15 32-37) However, due to the lack of understanding of catalyst function, SnO, arrays do not allow deliberate chemical control of the response of elements in the arrays nor reproducibility of response from array to array Surface acoustic wave resonators are extremely sensitive to both mass and acoustic impedance changes of the coatings in array elements, but the signal transduction mechanism involves somewhat complicated electronics, requinng frequency measurement to 1 Hz while sustaining a 100 MHz Rayleigh wave in the crystal (Grate and Abraham (1991) Sens Act B 3 85-11 1, Grate et al (1993) Anal Chem 65 1868-1881) Attempts have been made to construct sensors with conducting polymer elements that have been grown electrochemically through nominally identical polymer films and coatings (Pearce et al (1993) Analyst 118 371-377,

Shurmer et al (1991) Sens Act B 4 29-33, Topart and Josowicz (1992) J Phys Chem 96 7824-7830, Charlesworth et al (1993) 1 Phys Chem 97 5418-5423)

It is an object herein to provide a broadly responsive analyte detection sensor based on one or more chemiresistor elements Such elements are simply prepared and are readily modified chemically to respond to a broad range of

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-3PCTUS97/14070 analytes Such elements may also respond to temperature and current variation In addition, these sensors yield a rapid, low power, dc electrical signal in response to the fluid of interest, and their signals are readily integrated with software or hardware-based neural networks for purposes of analyte identification

Relevant Literature

Pearce el al (1993) Analyst J18 371-377 and Gardner et al (1994) Sensors and Actuators B 18-19 240-243 describe polypyrrole-based sensor arrays for monitoring beer flavor Shurmer (1990) U S Patent No 4,907,441 describes general sensor arrays with particular electrical circuitry

SUMMARY OF THE INVENTION The invention provides methods, apparatuses and systems for detecting and identifying analytes in fluids In one embodiment, the apparatuses include a chemical sensor comprising first and second conductive elements (e g, electrical leads) electrically coupled to a chemically sensitive resistor which provides an electrical path between the conductive elements The resistor comprises a plurality of alternating nonconductive regions (comprising a nonconductive organic polymer) and conductive regions (comprising a conductive material)

The electrical path between the first and second conductive elements is transverse to (/ c , passes through) said plurality of alternating nonconductive and conductive regions In use, the resistor provides a difference m resistance between the conductive elements when contacted with a fluid comprising a chemical analyte at a first concentration, than when contacted with a fluid comprising the chemical analyte at a second different concentration

The electrical path through any given nonconductive region is typically on the order of 100 ang.troms in length, providing a resistance of on the order of 100 mQ across the region Variability in chemical sensitivity from sensor to sensor is conveniently provided by qualitatively or quantitatively varying the

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-4composition of the conductive and/or nonconductive regions For example, in one embodiment, the conductive material in each resistor is held constant (e g, the same conductive material such as polypyrrole) while the nonconductive organic polymer varies between resistors (eg, different plastics such as polystyrene)

Arrays of such sensors are constructed with at least two sensors having different chemically sensitive resistors providing dissimilar differences in resistance An electronic nose for detecting an analyte in a fluid may be constructed by using such arrays m conjunction with an electrical measuring device electrically connected to the conductive elements of each sensor Such electronic noses may incorporate a variety of additional components including means for monitoring the temporal response of each sensor, assembling and analyzing sensor data to determine analyte identity, etc Methods of making and using the disclosed sensors, arrays and electronic noses are also provided

In another embodiment, the sensor for detecting the presence of a chemical analyte in a fluid comprises a chemically sensitive resistor electrically connected to an electrical measuring apparatus where the resistor is in thermal communication with a temperature control apparatus The chemically sensitive resistor composes a mixture of a nonconductive organic polymer and a conductive material compositionally different than said nonconductive organic polymer and provides an electrical path therethrough The chemically sensitive resistor provides varying electrical res'stances (R„) at varying temperatures (T_m) when contacted with a fluid comprising a particular chemical analyte

Such sensors also function to provide an electrical impedance Z_m at frequency when contacted with a fluid comprising a chemical analyte, where m is an integer greater than 1 and G_m does not equal 0 Finally, such sensors may also provide an electrical impedance Z^_n at frequency ω„ and temperature T„ when contacted with a fluid comprising a chemical analyte, where m and/or n is an

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-5PCT/US97/14070 integer greater than 1 The invention is also directed to systems and methods for employing such sensors for the detection of a chemical analyte in a fluid

BRIEF DESCRIPTION OF THE FIGURES

Fig 1A shows an overview of sensor design, Fig IB shows an overview of sensor operation, Fig 1C shows an overview of system operation

Fig 2 shows cyclic voltammogram of ι poly(pyrrole)-coated platinum electrode The electrolyte was ° iC M , (CyH^NJ* [CIOJ m acetonitrile, with a scan rate of 0 10 V s'

Fig 3A shows the optical spectrum of a spin coated poly(pyrrole) film that had been washed uith methanol to remove excess pynole and reduced phosphomolybdic acid Fig 3B shows the optical spectrum of a spin-coated poly(pyrrole) film on mdium-tin-oxidc after 10 potential cycles between +0 70 and -1 00 V vs SCE in 0 10 M [(C₄Hj)₄N]‘ [C1OJ‘ in acetonitrile at a scan rate ofO 10 V -s¹ The spectra were obtained in 0 10 M KCI - H₂O

Γig 4 A is a schematic of a sensor array sho' 'ing an enlargement of one of the modified ceramic capacitors used as sensing elements The response patterns generated by the sensor array described in Tabic 3 are displayed for Fig 4B acetone, Fig 4C benzene, and Fig 4D ethanol

Fig S is a principle component analysis of autoscalcd data from individual sensors containing different plasticizers The numbers in the upper right hand corner of each square refer to the different sensor elements descnbed in Table 3

Figs 6A and 6B are principle component analysis of data obtained from all sensors (Table 3) Conditions and symbols are identical to Figs 5A-5D Fig

6Λ shows data represented in the first three pnnciple components pci, pc2 and pc3, while Fig 6B shows the data when represented in pci, pc2, and pc4 A

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-6higher degree of discrimination between some solvents could be obtained by considering the fourth principle component as illustrated by larger separations between chloroform, tetrahydrofuran, ana isopropyl alcohol in Fig 6B

Fig 7A is a plot of acetone partial pressure (0) as a function of the first principle component, linear least square fit (—) between the partial pressure of acetone and the first principle component (P, = 8 26· pel + 83 4, R² = 0 989), acetone partial pressure (+) predicted from a multi-linear least square fit between the partial pressure of acetone and the first three principle components (P, = 8 26«pcl - 0 673«pc2 + 6 25«pc3 + 83 4, R² = 0 998) Fig 7B is a plot of the mole fraction of methanol, x_m, (0) in a methanol - ethanol mixture as a function of the first principle component, linear least square fit (-) between Xm and the first principle component (x_m = 0 112*pcl + 0 524, R² =

979), x_m predicted from a multi-linear least square fit (+) between x„ and the first three principle components (x„ = 0 112«pcl - 0 O3OO«pc2 - 0 0444«pc3 +

524, R²- 0 987)

Fig 8 is the resistance response of a poly(N-vinylpyrrolidone) carbon black (20 w/w% carbon black) sensor clement to methanol, acetone, and benzene The analyte was n ’reduced at t=60 s for 60 s Each trace is normalized by the resistance of the sensor element (approx 125Ω) before each exposure

Fig 9 is the first three principal components for the response of a carbon-black based sensor array with 10 element The non-conductive components of the carbon-black composites used are listed in Table 3, and the resistors were 20 w/w% carbon black

Fig 10 is a schematic illustration of the sensor employed to measure the effect of temperature on differential resistance responses to various chemical analytes

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Figures 11 A-l 1M are graphical representations of the data presented m Table 6 which is the average resistance measured by each sensor employed and for each of tne eight analytes tested

Figures 12A-12M are graphical representations of the data presented in Table 7 which is the normalized average resistance measured by each sensor employed and for each of the either analytes tested

DETAILED DESCRIPTION OF THE INVENTION

The invention provides sensor arrays for detecting an analyte in a fluid for use in conjunction with an electncal measuring apparatus These arrays comprise a plurality of compositionally different chemical sensors Each sensor comprises at least first and second conductive leads electrically coupled to and separated by a chemically sensitive resistor The leads may be any convenient conductive material, usually a metal, and may be interdigitized to maximize signal-t< -noise strength

The resistor composes a plurality of alternating nonconductive and conductive regions transverse to the electncal path between the conductive leads

Generally, the resistors are fabricated by blending a conductive matenal with a nonconductive organic polymer such that the electrically conductive path between the leads coupled to the resistor is interrupted by gaps of nonconductive organic polymer material For example, in a colloid, suspension or dispersion of particulate conductive matenal in a matrix of nonconductive organic polymer matenal, the matrix regions separating the particles provide the gaps The nonconductive gaps range in path length from about 10 to 1,000 angstroms, usually on the order of 100 angstroms providing individual resistance of about lc to 1,000 mfl, usually on the order of 100 πιΩ, across each gap The path length and resistance of a given gap is not constant but rather is believed to change as the nonconductive organic polymer of the region absorbs, adsorbs or imbibes an analyte Accordingly, the dynamic aggregate

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-8resistance provided by these gaps in a given resistor is a function of analyte permeation of the nonconductive regions In some embodiments, the conductive material may also contribute to the dynamic aggregate resistance as a function of analyte permeation (e g, when the conductive material is a conductive organic polymer such as polyprryole)

A wide variety of conductive materials and nonconductive organic polymer materials can be used Table 1 provides exemplary conductive materials for use m resistor fabrication, mixtures, such as of those listed, may also be used Table

2 provides exemplary nonconductive organic polymer materials, blends and copolymers, such as of the polymers listed here, may also be used Combinations, concentrations, blend stoichiometries, percolation thresholds, etc arc readily determined empirically by fabricating and screening prototype resistors (chemiresistors) as described below

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TABLE.!

<td> Maior Class</td><td> Examples</td>

<td> Organic Conductors</td><td> conducting polymers (poly(anilines), poly(thiophenes), poly(pyrroles), poly(acetylenes), etc)), carbonaceous materials (carbon blacks, graphite, coke, Cm, etc ), charge transfer complexes (tetramethylparaphenylenediamine- chloramle, alkali metal tetracyanoquinodimetnane complexes, tetrathiofulvalene halide complexes, etc), etc</td>

<td> Inorganic Conductors</td><td> metals and metal alloys (Ag, Au, Cu, Pt, AuCu alloy, etc), highly doped semiconductors (Si, GaAs, InP, MoS₂, TiO₂, etc), conductive metal oxides (In₂0j, SnOj, Na,PtjO₄, etc), superconductors (YBa₂CujO,, TI,Ba,Ca,Cu,O„ etc), etc</td>

<td> Mixed inorgamc/orgamc Conductors</td><td> Tetracyanoplatinatc complexes. Indium halocarbonyl complexes, stacked macrocychc comptexes, etc ¹</td>

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TABLE 2

<td> Maior Class</td><td> Examples</td>

<td> Main-chain carbon polymers</td><td> poly(dienes), poly(alkenes) poly(acrylics), poly(methacrylics), poly(vmyl e'l.ers), poly(vmyl thiocrhers), poly(vmyl alcohols), poly(vmyl ketones), poly(vmyl halides), poly(vinyl nitriles), poly(vmyl esters), poly(styrenes), poly(arylenes), etc</td>

<td> Main-chain acyclic heteroatom polymers</td><td> poly(oxides), poly(carbonates), poly(esters), poly(anhydndes), poly(urethanes), poly(sulfonates), poly(siloxanes), poly(sulfides), poly(thioesters), poly(sulfones), poly(sulfonamides), poiy(amides), poly(urcas), poly(phosphazener), poly(silanes), polvfsilazancs), etc</td>

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<td> Mam-chain heterocyclic polymers</td><td> poly(fUran tetracarboxylic acid diimides), poly(benzoxazoles), poly(oxadiazoles), poly(benzothiazmophenothiazines), poly(benzothiazoles), poly(pyrazinoqmnoxalines), poly(pyromelhtimi des), poly(quinoxalines), poly(benzimidazoles), poly(oxmdoles), poly(oxoisoindolines), poly(dioxoisoindolines), poly(tnazincs), poly(pyndazines), poly(pipcrazines), poly(pyndines), poly(pipendmes), poly(inazoles), poly(pyrazoles), poly(pyrrohdines), poly(carboranes), poly(oxabicyclononanes), poly(dibcnzofUrans), poly(phthalides), poly(acetals), poly(anhydndcs), carbohydrates, etc</td>

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-12The chemiresistors can be fabricated by many techniques such as, but not limited to, solution casting, suspension casting, and mechanical mixing In general, solution cast routes are advantageous because they provide homogeneous structures and ease of processing With solution cast routes, resistor elements may be easily fabricated by spin, spray or dip coating Since all elements of the resistor must be soluble, however, solution cast routes are somewhat limited in their applicability Suspension casting still provides the possibility of spin, spray or dip coating but more heterogeneous structures than with solution casting are expected With mechanical mixing, there are no solubility restrictions since it involves only the physical mixing of the resistor components, but device fabncation is more difficult since spin, spray and dip coating are no longer possib'e A more detailed discussion of each of these follows

For systems where both the conducting and non-conducting media or their reaction precursors are soluble in a common solvent, the chemiresistors can be fabricated by solution casting The oxidation of pyrrole by phosphomolybdic acid presented herein represents such a system In this reaction, the phosphomolybdic acid and pyrrole are dissolved in tetrahydrofuran (THF) and polymerization occurs upon solvent evaporation This allows for THF soluble non-conductive polymers to be dissolved into this reaction mixture thereby allowing the blend to be formed in a single step upon solvent evaporation The choice of non-conductive polymers in this route is, of course, limited to those that are soluble in the reaction media For the poly(pyrrole) case described above, preliminary reactions were performed in THF, but this reaction should be generalizable to other non-aqueous solvent such as acetonitrile or ether A vanety of permutations on this scheme are possible for other conducting polymers Some of these are listed below Certain conducting polymers, such as substituted poly(cyclooctatetraenes), are soluble in their undoped, non-conducting state in solvents such as THF or acetonitrile Consequently, the blends between the undoped polymer and plasticizing polymer can be formed

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-13PCT/US97/14072 from solution casting After which, the doping procedure (exposure to I₂ vapor, for instance) can be performed on the blend to render the substituted poly(cyclooctatetraene) conductive Again, the choice of non-conductive polymers is limited to those that are soluble in the solvents that the undoped conducting polymer is soluble m and to those stable to the doping reaction Certain conducting polymers can also be synthesized via a soluble precursor polymer In these cases, blends between the precursor polymer and the non-conducting polymer can first be formed followed by chemical reaction to convert the precursor polymer into the desired conducting polymer For instance poly(p-phcnylene vinylene) can be synthesized through a soluble su’.fomum precursor Blends between this sulfomum precursor and the non-conductive polymer can be formed by solution casting After which, the blend can be subjected to thermal treatment under vacuum to convert the sulfomum precursor to the desired poly(p-phenylene vinylene)

In suspension casting, one or more of the components of the resistor is suspended and the others dissolved in a common solvent Suspension casting is a rather general technique applicable to a wide range of species, such as carbon blacks or colloidal metals, which can be suspended in solvents by vigorous mixing or somcation In one application of suspension casting, the non-conductive polymer is dissolved in an appropriate solvent (such as THF, acetomtnle, water, etc) Colloidal silver is then suspended in this solution and the resulting mixture is used to dip coat electrodes

Mechanical mixing is suitable for all of the conductive/non-conductive combinations possible In this technique, the matenals are physically mixed in a ball-mill or other mixing device For instance, carbon black non-conductive polymer composites are readily made by ball-milling When the non-conductive polymer can be melted or significantly softened without decomposition, mechanical mixing at elevated temperature can improve the mixing process

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-14Altematively, composite fabrication can sometimes be improved by several sequential heat and mix steps

Once fabricated, the individual elements can be optimized for a particular application by varying their chemical make up and morphologies The chemical nature of the resistors determines to which analytes they will respond and their ability to distinguish different analytes The relative ratio of conductive to insulating components determines the magnitude of the response since the resistance of the elements becomes more sensitive to sorbed molecules as the percolation threshold is approached The film morphology is also important in determining response characteristics For instance, thin films respond more quickly to analytes than do thick ones Hence, with an empincal catalogue of information on chemically diverse sensors made with varying ratios of insulating to conducting components and by differing fabrication routes, sensors can be chosen that are appropriate for the analytes expected in a particular application, their concentrations, and the desired response times Further optimization can then be performed in an iterative fashion as feedback on the performance of an array under particular conditions becomes available

The resistor may itself form a substrate for attaching the lead or the resistor

For example, the structural rigidity of the resistors may be enhanced through a variety of techniques chemical or radiation cross-linking of polymer components (dicumyl peroxide radical cross-linking, UV-radiation cross-linking of poly(olefins), sulfur cross-linking of rubbers, e-beam cross-linking of Nylon, etc ), the incorporation of polymers or other materials into the resistors to enhance physical properties (for instance, the incorporation of a high molecular weight, high transition metal (Tm) polymers), the incorporation of the resistor elements into supporting matnees such as clays or polymer networks (forming the resistor blends within poly-(methylmethacrylate) networks or within the lamellae of montmomllonite, for instance), etc In another embodiment, the resistor is deposited as a surface layer on a solid matrix which provides means

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-15for supporting the leads Typically, the matrix is a chemically inert, nonconductivc substrate such as a glass or ceramic

Sensor arrays particularly well-suited to scaled up production are fabricated using integrated circuit (IC) design technologies For example, the chemiresistors can easily be integrated onto the front end of a simple amplifier interfaced to an A/D converter to efficiently feed the data stream directly into a neural network software or hardware analysis section Micro-fabncation techniques can integrate the chemiresistors directly onto a micro-chip which contains the circuitry for analogue signal conditionmg/processing and then data analysis This provides for the production of millions of incrementally different sensor elements in a single manufacturing step using ink-jet technology Controlled compositional gradients in the chemiresistor elements of a sensor array can be induced in a method analogous to how a color ink-jet printer deposits and mixes multiple colors However, in this case rather than multiple colors, a plurality of different polymers in solution which can be deposited are used A sensor array of a million distinct elements only requires a 1 cm x 1cm sized chip employing lithography at the 10 pm feature level, which is within the capacity of conventional commercial processing and deposition methods This technology permits the production of sensitive, small-sized, stand-alone chemical „^nsors

Preferred sensor arrays have a predetermined inter-sensor variation in the structure or composition of the nonconductive organic polymer regions The vanation may be quantitative and/or qualitative For example, the concentration of the nonconductive organic polymer in the blend can be varied across sensors Alternatively, a variety of different organic polymers may be used in different sensors An electronic nose for detecting an analyte in a fluid is fabricated by electncally coupling the sensor leads of an array of compositionally different sensors to an electrical measuring device The device measures changes in resistivity at each sensor of the array, preferably simultaneously and preferably

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-16over time Frequently, the device includes signal processing means and is used in conjunction with a computer and data structure for comparing a given response profile to a structure-response profile database for qualitative and quantitative analysis Typically such a nose comprises at least ten, usually at least 100, and often at least 1000 different sensors though with mass deposition fabrication techniques descnbed herein or otherwise known in the art, arrays of on the order of at least 10⁶ sensors are readily produced

In operation, each resistor provides a first electrical resistance between its conductive leads when the resistor's contacted with a first fluid comprising a chemical analyte at a first concentration, and a second electrical resistance between its conductive leads when the resistor is contacted with a second fluid comprising the same chemical analyte at a second different concentration Moreover, a resistor may provide a first electrical resistance when the resistor is contacted with a first fluid comprising a first chemical analyte at a concentration Cm and a second electrical resistance when the resistor is contacted with a second fluid comprising a second, different chemical analyte at concentration C„, wherein C_m and C_n may be the same or different The fluids may be liquid or gaseous in nature The first and second fluids may reflect samples from two different environments, a change in the concentration of an analyte in a fluid sampled at two time points, a sample and a negative control, etc The sensor array necessarily comprises sensors which respond differently to a change in an analyte concentration, z c , the difference between the first and second electrical resistance of one sensor is different from the difference between the first second electrical resistance of another sensor

In a preferred embodiment, the temporal response of each sensor (resistance as a function of time) is recorded The temporal response of each sensor may be normalized to a maximum percent increase and percent decrease in resistance which produces a response pattern associated with the exposure of the analyte By iterative profiling of known analytes, a structure-function database

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-17PCT/US97/14070 correlating analytes and response profiles is generated Unknown analyte may then be charactenzed or identified using response pattern companson and recognition algonthms Accordingly, analyte detection systems compnsing sensor arrays, an electncal measunng devise for detecting resistance across each chemiresistor, a computer, a data structure of sensor array response profiles, and a companson algonthm are provided In another embodiment, the electncal measuring device is an integrated cicuit compnsing neural network-based hardware and a digital-analog converter (DAC) multiplexed to each sensor, or a plurality of DACs, each connected to different sensor(s)

A wide vanety of analytes and fluids may be analyzed by the disclosed sensors, arrays and noses so long as the subject analyte is capable generating a differential response across a plurality of sensors of the array Analyte applications include broad ranges of chemical classes such as organics such as alkanes, alkenes, alkynes, dienes, alicyclic hydrocarbons, arenes, alcohols, ethers, ketones, aldehydes, carbonyls, carbamons, polynuclear aromaucs and derivatives of such organics, e g, halide derivatives, etc, biomolecules such as sugars, isoDrenes and isoprenoids, fatty acids and derivatives, etc Accordingly, commercial applications of the sensors, arrays and noses include environmental toxicology and remediation, biomedicine, matenals quality control, food and agncultural products monitoring, etc

The general method for using the disclosed sensors, arrays and electronic noses, for detecting the presence of an analyte in a fluid involves resistively sensing the presence of an analyte in a fluid with a chemical sensor compnsing first and second conductive leads clectncally coupled to and separated by a chemically sensitive resistor as descnbed above by measunng a first resistance between the conductive leads when the resistor is contacted with a first fluid comprising an analyte at a first concentration and a second different resistance when the resistor is contacted with a second fluid comprising the analyte at a second different concentration

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In another embodiment, the sensor for detecting the presence of a chemical analyte in a fluid comprises a chemically sensitive resistor electrically connected to an electncal measuring apparatus where the resistor is in thermal communication with a temperature control apparatus As described above, the che””CaPy sensitive res'stor(s) comprise a mixture of a ponconductwe orgamc polymer and a conductive material which is compositionally different than the nonconductive organic polymer The chemically sensitive resistor provides an electrical path through which electrical current may flow and a resistance (R) at a temperature (T) when contacted with a fluid comprising a chemical analyte

In operation, the chemically sensitive resistor(s) of the sensor for detecting the presence of a chemcial analyte m a fluid provide an electrical resistance (R_m) when contacted with a fluid comprising a chemical analyte at a particular temperature (T_m) The electrical resistance observed may vary as the temperature vanes, thereby allowing one to define a unique profile of electncal resistances at various different temperatures for any chemical analyte of interest !

For example, a chemically sensitive resistor, when contacted with a fluid comprising a chemical analyte of interest, may provide an electncal resistance R,,, at temperature T_m where m is an integer greater than 1, and may provide a different electncal resistance R„ at a different temperature T_n The difference between R,„ and ίΖ, is readily detectable by an electrical measuring apparatus

As such, the chemically sensitive resistor(s) of the sensor are in thermal communication with a temperature control apparatus, thereby allowing one to vary the temperature at which electncal resistances are measured If the sensor compnses an array of two or more chemically sensitive resistors each being in thermal communication with a temperature control apparatus, one may vary the temperature across the entire array (i c, generate a temperature gradient across the array), thereby allowing electncal resistances to be measured simultaneously at various different temperatures and various different resistor compositions For example, in an array of chemically sensitive resistors, one may vary the

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-19PCT/US97/14070 composition of the resistors in the horizontal direction across the array, such that, esistor composition in the vertical direction across the array remains constant One may then create a temperature gradient in the vertical direction across the arra&gt;, thereby allowing the simultaneous analysis of chemical analytes at different resistor compositions and different temperatures

Methods for placing chemically sensitive resistors in thermal communication with a temperature control apparatus are readily apparent to those skilled in the art and include, for example, attaching a heating element to the sensor and passing electrical current through said heating element The temperature range across wh.ch electrical resistances may be measured will be a function of the resistor composition, for example the melting temperature of the resistor components, the thermal stability of the analyte of interest or any other component of the system, and the like For the most part, the temperature range across which electrical resistance will be measured will be about 20°C to 80°C , preferably from about 22°C to about 70°C and more preferably from about 22°C to 65°C

In yet another embodiment, rather than subjecting the sensor to a direct electrical current and measuring the true elect«cal resistance through the chemically sensitive resistor(s), the sensor can be subjected to an alternating electrical current at different frequencies to measure impedance Impedance is the apparent resistance in an alternating electrical current as compared to the true electrical resistance in a direct current As such, the present invention is also directed to a sensor for detecting the presence of a chemical analyte in a fluid, said sensor comprising a chemically sensitive resistor electrically connected to an electrical measuring apparatus, said chemically sensitive resistor comprising a mixture of nonconductive organic polymer and a conductive matenal compositionally different than said nonconductive organic polymer and wherein said resistor provides (a) an electrical path through said mixture of nonconductive organic polymer and said conductive material, and (b) an

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-20PCTUJS97/14O70 electrical impedance Z_e at frequency o_m when contacted with a fluid comprising said chemical analyte, where m is an integer greater than 1 and ω_π does not equal 0 For measuring impedance as a function of frequency, the frequencies employed will generally range from about 1 Hz to 5 GHz, usually from about 1

MHz to 1 GHz more usually from about 1 MHz to 10 MHz and preferably from about 1 MHz to 5 MHz Chemical analytes of interest will exhibit unique impedance characteristics at varying alternating current frequencies, thereby allowing one to detect the presence of any chemical analyte of interest m a fluid by measuring Z_m at alternating frequency

For performing impedance measurements, one may employ virtually any impedance analyzer known in the art Preferably, a Schlumberger Model 1260 Impedance/Gain-Phase Analyzer (Schlumberger Technologies, Famborough, Hampshire, England) with approximately 6 meh RG174 coaxial cables is employed In such an apparatus, the resistor/sensor is held in an A1 chassis box to shield them from external electronic noise

In still another embodiment of the present invention, one may vary both the frequency co_m of the electrical current employed and the temperature T„ and measure the electrical impedance Z_mi„, thereby allowing for the detection of the presence of a chemical analyte of interest As such, the present invention is also directed to a sensor for detecting the presence of a chemical analyte in a fluid, said sensor comprising a chemically sensitive resistor electrically connected to an electncal measuring apparatus and being in thermal communication with a temperature control apparatus, said chemically sensitive resistor comprising a mixture of nonconductive organic polymer and a conductive material compositionally different than said nonconductive organic polymer, wherein said resistor provides (1) an electncal path through said mixture of nonconductive organic polymer and said conductive matenal, and (2) an electrical impedance Z^„ at frequency u_m and temperature T„ when contacted with a fluid comprising said chemical analyte, where m and/or n is an integer

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-21greater than 1 For measuring impedance as a function of frequency and temperature, the frequencies employed will generally not be higher than 10 MHz, preferably not higher than 5 MHz Chemical analytes of interest will exhibit unique impedance characteristics at varying alternating current frequencies and varying temperatures, thereby allowing one to detect the presence of any chemical analyte of interest m a fluid by measuring at frequency to_m and temperature T„

The present invention is also directed to systems and methods for employing the above described sensors for detecting the presence of a chemical analyte m a ' fluid

The following examples are offered by way of illustration and not by way of limitation

EXAMPLES

I. Sgnsor Arrays

Poly(pyrrole) films used for conductivity, electrochemical, and optical measurements were prepared by injecting equal volumes of N₂-purged solutions of pyrrole (1.50 mmoles in 4 0 ml dry tetrahydrofuran) and phosphomolybdic acid (0 75 mmoles in 4 0 ml tetrahydrofuran) into a N₂purged test tube Once the two solutions were mixed, the yellow phosphomolybdic acid solution turned dark green, with no observable precipitation for several hours This solution was used for film preparation within an hour of mixing.

ScnsorFabncation

Plasticized poly(pyrrole) sensors were made by mixing two solutions, one of which contained 0 29 mmoles pyrrole in 5 0 ml tetrahydrofuran, with the other containing 0 25 mmoles phosphomolybdic acid and 30 mg of plasticizer

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WO 9807724

-22in 5 0 ml of tetrahydrofuran The mixture of these two solutions resulted in a w w ratio of pyrrole to plasticizer of 2 3 An inexpensive, quick method for crating the chemiresistor array elements was accomplished by effecting a cross sectional cut through commercial 22 nF ceramic capacitors (Kemet

Electronics Corporation) Mechanical slices through these capacitors revealed a senes of interdigitated metal lines (25% Ag 75% Pt), separated by 15 μτη, that could be readily coated with conducting polymer The monomer - plasticizer - oxidant solutions were then used to dip coat interdigitated electrodes in order to provide a robust electrical contact to the polymenzed organic films After polymenzation was complete, the film was insoluble and was rinsed with solvent (tetrahydrofuran or methanol) to remove residual phosphomolybdic acid and unreacted monomer The sensors were then connected to a commercial bus strip, with the resistances of the various chemiresistor elements readily monitored by use of a multiplexing digital ohmmeter

Instrumentation

Optical spectra were obtained on a Hewlett Packard 8452A spectrophotometer, interfaced to an IBM XT Electrochemical experiments were performed using a Princeton Applied Research Inc 173 potentiostat/175 universal programmer All electrochemical experiments were performed with a Pt flag auxiliary and a saturated calomel reference electrode (SCE) Spin-coating was performed on a Headway Research Inc photoresist spin coater Film thicknesses were determined with a Dektak Model 3030 profilometer Conductivity measurements were performed with an osmiumtipped four point probe (Alessi Instruments Inc , tip spacing = 0 050, tip radii = 0 010) Transient resistance measurements were made with a conventional multimeter (Fluke Inc , Hydra Data Logger Meter)

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Principle Component Analysis and Multi-linear Least Square Fits A data set obtained from a single exposure of the array to an odorant produced a set of descriptors (i e , resistances), d. The data obtained from multiple exposures thus produced a data matrix D where each row, designated by j, consisted of n descriptors describing a single member of the data set (i e , a single exposure to an odor) Since the baseline resistance and the relative changes in resistance varied among sensor,s the data matrix was autoscaled before further processing (Hecht (1990) Mathematics in Chemistry An Introduction to Modem Methods (Prentice Hall, Englewood Cliffs, NJ)) In this preprocessing technique, all the data associated with a single descriptor (i e , a column m the data matrix) were centered around zero with unit standard deviation d = (d„ - d,)/o, (1) where d, is the mean value for descriptor i and σ, is the corresponding standard deviation

Principle component analysis (Hecht (1990)) was performed to determine linear combinations of the data such that the maximum variance [defined as the square of the standard deviation] between the members of the data set was obtained in n mutually orthogonal dimensions The linear combinations of the data resulted in the largest variance [or separation] between the members of the data set in the first principle component (pci) and produced decreasing magnitudes of variance from the second to the n¹¹' principle component (pc2 pen) The coefficients required to transform the autoscaled data into principle component space (by linear combination) were determined by multiplying the data matrix, D, by its transpose, D^T(i e , diagnolizing the matrix) (Hecht (1990) Mathmatics in Chemistry An Introduction to Modem Methods (Prentice Hall, Englewood Cliffs, NJ))

R = D^T«D (2) n

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This operation produced the correlation matrix, R whose diagonal elements were unity and whose off-diagonal elements were the correlation coefficients of the data The total vanance m the data was thus given by the sum of the diagonal elements in R The n eigenvalues, and the corresponding n eigenvectors, were then determined for R Each eigenvector contained a set of n coefficients which were used to transform the data by linear combination into one of its n principle components The corresponding eigenvalue yielded the fraction of the total vanance that was contained in that pnnciple component This operation produced a principle component matrix, P, which had the same dimensions as the original data matrix Under these conditions, each row of the matrix P was still associated with a particular odor and each column was associated with a particular pnnciple component

Since the values m the principle component space had no physical meaning, it was useful to express the results of the principle component analysis in terms of physical parameters such as partial pressure and mole fraction This was achieved via a multi-linear least square fit between the principle component values and the corresponding parameter of interest A multi-linear least square fit resulted in a linear combination of the pnnciple components which yielded the best fit to the corresponding parameter value Fits were achieved by appending a column with each entry being unity to the pnnciple component matrix P, with each row, j, corresponding to a different parameter value (e g , partial pressure), v_;, contained in vector V The coefficients for the best multi-linear fit between the principle components and parameter of interest were obtained by the following matrix operation

C = (Ρ^τ·Ρ)'·Ρ^τ·ν (3) where C was a vector containing the coefficients for the linear combination

A key to our ability to fabricate chemically diverse sensing elements was the preparation of processable, air stable films of electrically conducting organic

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-25PCTTJS97/14079 polymers This was achieved through the controlled chemical oxidation of pyrrole (PY) using phosphomolybdic acid (Η₃ΡΜο_πΟ₄₀) (20 in tetrahydrofuran

PY - PY*⁺ + e (4)

2PY'+ - PY, + 2H4 (5)

H₃PMo_nO4₀ + 2e- + 2H+ - H₅P^o„O₄₀ (6)

The redox-driven or electrochemically-induced polymerization of pyrrole has been explored previously, but this process typically yields insoluble, intractable deposits of poly(pyrrole) as the product (Salmon et al (1982) J

Polym Sci , Polym Lett 20 187-193) Our approach was to use low concentrations of the HiPMo^O^ oxidant (E° = +0 36 V vs SCE) (Pope (1983) Heteropoly and Isapoly Oxometalates (Springer-Verlag, New York), Chap 4) Since the electrochemical potential of PY⁺’/PY is more positive (E° = +1 30 V vs SCE) (Andneux et al (1^^^Q)J Am Chem Soc

112 2439-2440) than that of HjPMouO^/HjPMojO^, the equilibrium concentration of PY⁺·, and thus the rate of polymerization, was relatively low in dilute solutions (0 19 M PY, 0 09 M H₃PMo_nO₄₀). However, it has been shown that the oxidation potential of pyrrole oligomers decreases from +1 20 V to +0 55 to +0 26 V vs SCE as the number of units increase from one to two to three, and that the oxidation potential of bulk poly(pyrrole) occurs at -0 10 V vs SCE (Diaz et al (1981) J Electroanal Chem 121 355-361) As a result, oxidation of pyrrole trimers by phosphomolybdic acid is expected to be thermodynamically favorable This allowed processing /

of the monomer-oxidant solution (t e , spin coating, dip coating, introduction of plasticizers, etc ), aftei which time polymerization to form thm films was simply effected by evaporation of the solvent The dc electrical conductivity of poly(pyrrole) films formed by this method on glass slides, after rinsing the films with methanol to remove excess phosphomolybdic acid and/or monomer, was on the order of 15 - 30 S-cm'¹ for films ranging from 40 - 100 nm in thickness

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- The poly(pyrrole) films produced m this work exhibited excellent electrochemical and optical properties For example, Fig 2 shows the cyclic voltammetnc behavior of a chemically polymerized poly(pyrrole) film following ten cycles from -1 00 V to +0 70 V vs SCE The cathodic wave at -0 40 V corresponded to the reduction of poly(pyrrole) to its neutral, nonconducting state, and the anodic wave at -0 20 V corresponded to the reoxidation of poly(pyrrole) to its conducting state (Kanazawa et al (1981) Synth Met 4 119-130) The lack of additional faradaic current, which would result from the oxidation and reduction of phosphomolybdic acid in the film, suggests that the Keggm structure of phosphomolybdic acid was not present in the film anions (Bidan et al (1988) J Electroanal Chem 251 297-306) and implies that MoO/, or other anions, served as the poly(pyrrole) countenons in the polymerized films

Fig 3A shows the optical spectrum of a processed polypyrrole film that had been spm-coated on glass and then rinsed with methanol The single absorption maximum was characteristic of a highly oxidized poly(pyrrole) (Kaufman et al (1984) Phys Rev Lett 53 1005-1008), and the absorption band at 4 0 eV was charactenstic of an interband transition between the conduction and valence bands The lack of other bands in this energy range was evidence for the presence of bipolaron states (see Fig 3A), as have been observed in highly oxidized poly(pyrrole) (Id) By cycling the film in 0 10 M [(C₄H₉)₄N]⁺[C1OJ - acetonitrile and then recording the optical spectra in 0 10 M KC1 - H₂O, it was possible to observe optical transitions charactenstic of polaron states in oxidized poly(pyrrole) (see Fig 3B) The polaron states have been reported to produce three optical transitions (Id ), which were observed at 2 0, 2 9, and 4 1 eV m Fig 3B Upon reduction of the film (c f Fig 3B), an increased intensity and a blue shift m the 2 9 eV band was observed, as expected for the π-π* transition associated with the

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-27PCT/US97/14077 pyrrole units contained in the polymer backbone (Yakusht et al (1983) J Chem Phys 79 4774-4778)

As described in the experimental section, various plasticizers were introduced 5 into the polymer films (Table 3)

<td></td><td> Table 3</td><td> Plasticizers used in array elements*</td>

<td></td><td> sensor</td><td> plasticizer</td>

<td></td><td> 1</td><td> none</td>

<td> 10</td><td> 2</td><td> none**</td>

<td></td><td> 3</td><td> poly(styrene)</td>

<td></td><td> 4</td><td> poly(styrene)</td>

<td></td><td> 5</td><td> poly(styrene)</td>

<td></td><td> 6</td><td> poly(a-methyl styrene)</td>

<td> 15</td><td> 7</td><td> poly(styrene-acrylomtnle)</td>

<td></td><td> 8</td><td> poly(styrene-maleic anydride)</td>

<td></td><td> 9</td><td> poly(styrene-allyl alcohol)</td>

<td></td><td> 10</td><td> poly(vmyl pyrrolidone)</td>

<td></td><td> 11</td><td> poly(vinyl phenol)</td>

<td> 20</td><td> 12</td><td> poly(vinyl butral)</td>

<td></td><td> 13</td><td> poly(vinyl acetate)</td>

<td></td><td> 14</td><td> poly(carbonatc)</td>

Sensors contained 2 3 (w w) ratio of pyrrole to plasticizer Film not rinsed to remove excess phosphomolybdic acid

These inclusions allowed chemical control over the binding properties and 30 electrical conductivity of the resulting plasticized polymers Sensor arrays consisted of as many as 14 different elements, with each element synthesized to produce a distinct chemical composition, and thus a distinct sensor

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-28response, for its polymer film The resistance, R, of each film-coated individual sensor was automatically recorded before, during, and after exposure to various odorants A typical tnal consisted of a 60 sec rest period in which the sensors were exposed to flowing air (3 0 liter-min *), a 60 sec exposure to a mixture of air (3 0 liter-mm *) and air that had been saturated with solvent (0 5 -3 5 liter-min'¹), and then a 240 sec exposure to air (3 0 liter-min'¹)

In an initial processing of the data, presented in this paper, the only information used was the maximum amplitude of the resistance change divided by the initial resistance, AIR._ai/R,, of each individual sensor element Most of the sensors exhibited either increases or decreases in resistance upon exposure to different vapors, as expected from changes in the polymer properties upon exposure to different types chemicals (Topart and Josowicz (1992)7 Phys Client 96 7824-7830, Charlesworth et al (1993)7 Phys

Client 97 5418-5423) However, in some cases, sensors displayed an initial decrease followed by an increase in resistance in response to a test odor Since the resistance of each sensor could increase and/or decrease relative to its initial value, two values of AR^./R, were reported for each sensor The source of the bi-directional behavior of some sensorlodor pairs has not yet been studied in detail, but in most cases this behavior arose from the presence of water (which by itself induced rapid decreases in the film resistance) m the reagent-grade solvents used to generate the test odors of this study The observed behavior in response to these air-exposed, water-containing test solvents was reproducible and reversible on a given sensor array, and the environment was representative of many practical odor sensing applications in which air and water would not be readily excluded

Figs 4B-4D depict representative examples of sensor amplitude responses of a sensor array (see, Table 3) In this experiment, data were recorded for

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-29three separate exposures to vapors of acetone, benzene, and ethanol flowing m air The response patterns generated by the sensor array described in Table 3 are displayed for (B) acetone, (C) benzene, and (D) ethanol The sensor response was defined as the maximum percent increase and decrease of the resistance divided by the initial resistance (gray bar and black bar, respectively) of each sensor upon exposure to solvent vapor In many cases sensors exhibited reproducible increases and decreases in resistance An exposure consisted of (i) a 60 sec rest period in which the sensors were exposed to flowing air (3 0 lit^^-^m'), (n) a 60 sec exposure to a mixture of air (3 0 liter-min’) and air that had been saturated with solvent (0 5 liter-min '), and (m) a 240 sec exposure to air (3 0 liter-min ') It is readily apparent that these odorants each produced a distinctive response on the sensor array In additional experiments, a total of 8 separate vapors (acetone, benzene, chloroform, ethanol, isopropyl alcohol, methanol, tetrahydrofuran, and ethyl acetate), chosen to span a range of chemical and physical characteristics, were evaluated over a five-day period on a I4-element sensor array (Table 3) As discussed below, each odorant could be clearly and reproducibly identified from the others using this sensor apparatus

Principle component analysis (Hecht (1990) Mathematics in Chemistry An Introduction to Modem Methods (Prentice Hall, Englewood Cliffs, NJ)) was used to simplify presentation of the data and to quantify the distinguishing abilities of individual sensors and o’ the array as a whole In this approach, linear combinations of the AR^/R, data for the elements in the array were constructed such that the maximum variance (defined as the square of the standard deviation) was contained in the fewest mutually orthogonal dimensions This allowed representation of most of the information contained in data sets shown in Figs 4B-4D m two (or three) dimensions The resulting clustering, or lack thereof, of like exposure data m the new

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-30dimensional space was used as a measure of the distinguishing ability, and of the reproducibility, of the sensor array

In order to illustrate the variation in sensor response of individual sensors that 5 resulted from changes in the plasticizing polymer, pnnciDle component analysis was performed on the individual, isolated responses of each of the 14 individual sensor elements in a typical array (Fig 5) Data were obtained from multiple exposures to acetone (a), benzene (b), chloroform (c), ethanol (e), isopropyl alcohol (i), methanol (m), tetrahydrofuran (t), or ethyl acetate (@) over a period of five days with the test vapors exposed to the array in various sequences The numbers of the figures refer to the sensor elements described in Table 3 The units along the axes indicate the amplitude of the principle component that was used to describe the particular data set for an odor The black regions indicate clusters corresponding to a single solvent which could be distinguished from all others, gray regions highlight data of solvents whose signals overlapped with others around it Exposure conditions were identical to those in Fig 4

Since each individual sensor produced two data values, principle component analysis of these responses resulted in only two orthogonal principal components, pci and pc2 As an example of the selectivity exhibited by an individual sensor element, the sensor designated as number 5 in Fig 5 (which was plasticized with poly(styrene)) confused acetone with chloroform, isopropyl alcohol, and tetrahydrofuran It also confused benzene with ethyl acetate, while easily distinguishing ethanol and methanol from all other solvents Changing the plasticizer to poly (α-methyl styrene) (sensor number 6 in Fig 5) had little effect on the spatial distribution of the responses with respect to one another and with respect to the origin Thus, as expected, a rather slight chemical modification of the plasticizer had little effect on the relative variance of the eight test odorants In contrast, the addition of a

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-31PCT/US9//14070 cyano group to the plasticizer, in the form of poly(styrene-acrylomtrile), (sensor number 7 m Fig 5), resulted m a larger contribution to the overall variance by benzene and chloroform, while decreasing the contribution of ethanol Changing the substituent group in the plasticizer to a hydrogen bonding acid (poly(styrene-allyl alcohol), sensor number 9 in Fig 5) increased the contribution of acetone to the overall variance while having little effect on the other odors, with the exception of confusing methanol and ethanol These results suggest that the behavior of the sen' n be systematically altered by varying the chemical composition o, e plasticizing polymer

Figs 6A and 6B show the principle component analysis for all 14 sensors described in Table 3 and Figs 4 and 5 When the solvents were projected into a three dimensional odor space (Fig 6A or 6B), all eight solvents were easily distinguished with the specific array discussed herein Detection of an individual test odor, based only on the criterion of observing -1 % ARm/R, values for all elements m the array, was readily accomplished at the parts per thousand level with no control over the temperature or humidity of the flowing air Further increases in sensitivity are likely after a thorough utilization of the temporal components of the AR^/R, data as well as a more complete characterization of the noise in the array

We have also investigated the suitability of this sensor array for identifying the components of certain test mixtures This task is greatly simplified if the array exhibits a predictable signal response as the concentration of a given odorant is varied, and if the responses of various individual odors are additive (i e , if superposition is maintained) When a 19-element sensor array was exposed to a number, n, of different acetone concentrations in air, the (CH₃);CO concentiation was semi-quantitavely predicted from the first principle component This was evident from a good linear least square fit

Ii

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-32PCT/US97/14070 through the first three principle components (see Fig 7A for the linear least square fit for the first principle component)

The same sensor array was also able to resolve the components in various test methanol-ethanol mixtures (Momis et al (1942) Can J Res B 20 207-211

As shown in Fig 7B, a linear relationship was observed between the first principle component and the mole fraction of methanol in the liquid phase, x_m, m a CH₃OH-C₂HjOH mixture, demonstrating that superposition held for this mixture/sensor array combination Furthermore, although the components m the mixture could be predicted fairly accurately from just the first principle component, an increase in the accuracy could be achieved using a multi-linear least square fit through the first three principle components This relationship held for CH₃OH/(CH3OH + C₂H₅OH) ratios of 0 to 10 in air-saturated solutions of this vapor mixture The conducting polymer-based sensor arrays could therefore not only distinguish between pure test vapors, but also allowed analysis of concentrations of odorants as well as analysis of binary mixtures of vapors

In summary, the results presented herein advance the area of analyte sensor design A relatively simple array design, using only a multiplexed lowpower dc electrical resistance readout signal, has been shown to readily distinguish between various test odorants Such conducting polymer-based arrays are simple to construct and modify, and afford an opportunity to effect chemical control over the response pattern of a vapor For example, by increasing the ratio of plasticizer to conducting polymer, it is possible to approach the percolation threshold, at which point the conductivity exhibits a very sensitive response to the presence of the sorbed molecules Furthermore, producing thinner films will afford the opportunity to obtain decreased response times, and increasing the number of plasticizing polymers and polymer backbone motifs will likely result in increased diversity among

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-33PCT/US97/14070 sensors This type of polymer-based array is chemically flexible, is simple to fabricate, modify, and analyze, and utilizes a low power dc resistance readout signal transduction path to convert chemical data into electrical signals It provides a new approach to broadly-rcsponsive odor sensors for fundamental and applied investigations of chemical mimics for the mammalian sense of smell Such systems are useful for evaluating the generality of neural network algorithms developed to understand how the mammalian olfactory system identifies the directionality, concentration, and identity of various odors

Fabrication and Testing of Carbon Black-based Sensor Arrays.

Sensor Fabrication

Individual sensor elements were fabricated m the following manner Each non-conductive polymer (80 mg, see Table 4) was dissolved in 6 ml of THF

TABLE 4

<td> Sensor #</td><td> Non-Conductive Polymer</td>

<td> 1</td><td> poly(4-vinyl phenol)</td>

<td> 2</td><td> poly(styrene - allyl alcohol)</td>

<td> 3</td><td> poly(a-methyl styrene)</td>

<td> 4</td><td> poly(vmyl chloride - vinyl acetate)</td>

<td> 5</td><td> poly(vinyl acetate)</td>

<td> 6</td><td> poly(N-vinyl pyrrolidone)</td>

<td> 7</td><td> poly(bisphenol A carbonate)</td>

<td> 8</td><td> poly(styrene)</td>

<td> 9</td><td> poly(styrene-maleic anhydride)</td>

<td> 10</td><td> poly(sulfone)</td>

Then, 20 mg of carbon black (BP 2000, Cabot Corp ) were suspended with 30 vigorous mixing Interdigitated electrodes (the cleaved capacitors previously

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-34PCT/US97/14070 described) were then dipped into this mixture and the solvent allowed to evaporate A series of such sensor elements with differing non-conductive polymers were fabricated and incorporated into a commercial bus strip which ahowea the chemiresistors to be easily monitored with a multiplexing ohmmeter

Sensor Array Testing

To evaluate the performance of the carbon-black based sensors, arrays with as many as 20 elements were exposed to a series of analytes A sensor exposure consisted of (1) a 60 second exposure to flowing air (6 liter mm-I), (2) a 60 second exposure to a mixture of air (6 liter min-1) and air that had been saturated with the analyte (0 5 liter min-1), (3) a five minute recovery period during which the sensor array was exposed to flowing air (6 liter min-1) The resistance of the elements were monitored during exposure, and depending on the thickness and chemical make-up of the film, resistance changes as large as 250% could be observed m response to an analyte In one experiment, a 10 element sensor array consisting carbon-black composites formed with a senes of non-conductive polymers (see Table 4) was exposed to acetone, benzene, chloroform, ethanol, hexane, methanol, and toluene over a two day period A total of 58 exposures to these analytes were performed m this time penod In all cases, resistance changes in response to the analytes were positive,and with the exception of acetone, reversible (see Fig 8) The maximum positive deviations were then subjected to principal component analysis in a manner analogous to that described for the poly(pyrrole) based sensor Fig 9 shows the results of the principal component analysis for the entire 10-element array With the exception of overlap between toluene with benzene, the analytes were distinguished from one and other

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Π Temperature Variance (Experiment 11

Sensor Fabncst.sn into 15 ml of benzene, was dissolved 160 mg of polymer (either poly(styrene) and poly(ethylene-co-vmyl acetate) and to this was added 40 mg of carbon black forming a suspension The sensor substrate was a 10 mm by 25 mm glass slide onto which had been evaporatedtwo gold pads approximately 1000 angstroms thick. These gold pads entirely covered one face of the slide with the exception of a 5 mm wide section across the middle of one face of the slide The glass slide was rapidly dipped into the polymer suspension of carbon black 5-10 fmes to coat the slide with the polymer composite The face opposite the gold pads was wiped clean of deposits ,

The heating element was made from 24 gauge mckle chromium wire which was bent into a zig-zag shape with each turn being equal m length to the width of the sensor element Wire tails were allowed to extend about 1 cm from opposite sides of the sensors for external electrical connections The total length of the heating elements was approximately 7 cm The heating element was attached to the back (the face opposite the gold pads) with epoxy (able to withstand 120°C) by sandwiching it between the sensor and another (uncoated) glass slide of the same size as the sensor The final sensor configuration was allowed to stand 5-10 days before use The heating elements were heated by passing 08,12orl6Aof current through the elements '

Data Collection

Each sensor was placed in the flow chamber individually, with all electrical connections in place, and allowed to equilibrate to the background air flow until a stable baseline was achieved Eacn exposure consisted of taking data for 60 seconds to establish a baseline, followed by a 60 second exposure to

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-36PCT/US97/14070 the analyte vapor stream and finally followed by a five minute recovery period before the next cycle began For both sensors, data were collected for exposures to chloroform and benzene at four different temperatures for each solvent First, data were collected for analyte exposure while at ambient temperature (about 22C, 1 e , temperature 1), followed by the temperature associated with passing 0 8 A of electrical current through the heating element (-temperature 2), then 1 2 A (“temperature 3”) and then 1 6 A of current (“temperature 4) (the latter temperatures being undetermined) The sensors were allowed to equilibrate at each new temperature for 15 minutes before data collection was initialed Three exposures at each current were taken

Pat? Analysis

The data streams were saved to the computer dunng the exposures to the analyte Baseline resistances were taken by averaging the resistance data during the 20 seconds prior to exposure to the analyte The maximum resistance was extracted from the data stream and the change in resistance between baseline and maximum resistance was calculated (AR) This AR value was divided by the baseline resistance (R) and multiplied by 100 to express the response as a percentage The percent responses for the three exposures at each temperature were averaged to obtain one value for each temperature/solvent combination The responses for each vapor at the four temperatures tested were normalized by summing the four values and dividing each response by this sum The results of these experiments are presented in Table 5

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<td></td><td> TABLES</td><td></td>

<td> Polymer composition/analvte</td><td> (AR/R) —QQ</td><td> Normalized</td>

<td> A Eoy^Yrene/Choroform</td><td></td><td></td>

<td> temperature 1</td><td> 0 60770905</td><td> 0.23762355</td>

<td> temperature 2</td><td> 0 69325945</td><td> 0 27107507</td>

<td> temperature 3</td><td> 0 75494462</td><td> 0 29519491</td>

<td> temperature 4</td><td> 0 50153141</td><td> 0 19610647</td>

<td> Sum</td><td> 2 55744454</td><td></td>

<td> B PolyfstyieneVBenzene</td><td></td><td></td>

<td> temperature 1</td><td> 0 33986648</td><td> 0 38334474</td>

<td> temperature 2</td><td> 0 23028222</td><td> 0 25974165</td>

<td> temperature 3</td><td> 0 14179893</td><td> 0 15993891</td>

<td> temperature 4</td><td> 0 17=463419</td><td> 0 19(69747</td>

Sum 0 88658181

C EoLy£elhylen£-co-v^yLaceareVChioro[orm

<td> temperature 1</td><td> 33 5395398</td><td> 0 63563285</td>

<td> temperature 2</td><td> 12 1030896</td><td> 0 22937468</td>

<td> temperature 3</td><td> 5 71049748</td><td> 0 1082239</td>

<td> temperature 4</td><td> 1 41245945</td><td> 0 02676857</td>

<td> Sum</td><td> 52 7655863</td><td></td>

D Poly(ethvlene-co-vinyl acetateVBenzene

<td> temperature 1</td><td> 10 236522</td><td> 0 54522797</td>

<td> temperature 2</td><td> 5 66926122</td><td> 0 30196192</td>

<td> temperature 3</td><td> 2 37495836</td><td> 0 12649743</td>

<td> temperature 4</td><td> 0 49401403</td><td> 0 02631267</td>

<td> Sum</td><td> 18 7747556</td><td></td>

The results in Table 5 demonstrate that both the poly(styrene) and poly(ethylene-co-vinyl acetate) sensors provided different electrical resistances at different temperatures with the two analytes tested Moreover, the results presented m Table 5 demonstrate that different analytes provide different, unique' patterns of electncal resistance at the various temperatures tested Therefore, these data demonstrate that by varying the temperature at

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-38PCT/US97/14070 which the electrical resistance readings are obtained from the sensor element, one is able to identify a unique pattern of electrical resistances for any analyte of interest, thereby allowing for the identification of an analyte of interest

III Temperature Variance (Experiment 2)

Three sensor composites were used These were poly(ethylene-co-vinyl acetate), polyethylene oxide), and poly(4-vinyl phenol) These composites were blended with conductive carbon black in a 1 4 carbon black to polymer ratio The composite material was coated onto a glass slide which had evaporated gold tabs as electrical contacts To the underside of the glass slide (the side without the polymer film) was affixed nickel-chromium heating wire using low vapor pressure/high temperature epoxy The wire was bent into a zig zag shape and affixed so that the main heating area was directly opposite the region between the gold tabs Next was attached the underside of the heating wire a plain (not coated with gold or polymer) glass slide of the same dimension as the substrate slide In this configuration the heating wire was sandwiched between two glass slides A thin thermocouple was glued to the second glass slide on the opposite side from the heating wire Therefore, the temperature detection was made at a similar orientation to the heater as the polymer composite film (See Figure 10)

The current for the resistive heaters was supplied by one common power supply The resistive heaters were wired m parallel with the power supply There was a rheostat (variable resistor) in senes with each branch of the parallel circuit This way the amount of current to each heater, and subsequently its temperature, could be controlled The temperatures were measured by the attached thermocouples These temperatures were not monitored continuously, but were recorded before an experiment began and at selected times during the experiment Once equilibrium was reached, the temperature of the sensor/heater did not deviate more than 0 1 °C over the

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-39PCT/US97/14070 course of minutes and did not deviate more than l^eC over the course of each experiment (about 3 hours)

Multiple copies of each sensor/heater were made and placed into a flow chamber For polyethylene oxide), five sensors were made, sensors 4, 6, 9, 15, and 16 Sensors 15 and 16 were heated, with sensor 15 being set to higher temperatures Sensors 4, 6, and 9 were not heated For polyethylene - co - vinyl acetate), four sensors were made, sensors 3, 8, 17, and 20 Sensors 17 and 20 were heated, with sensor 17 being set to higher temperatures Sensors 3 and 8 were not heated For poly(4-vmyl phenol), four sensors were made, sensors 5, 7, 18, and 19 Sensors 18 and 19 were heated, with sensor 18 being set to higher temperatures Sensors 5 and 7 were not heated

In these experiments, exposures were made to eight different solvents benzene, chloroform, toluene, cyclohexane, hexane, 2-propanol, ethanol, and methanol Each solvent was exposed at a concentration of 900 ppm In every experiment, the solvents were exposed to the sensors three times in the order listed above The average of these three trials was taken and the standard deviation calculated

Six experiments were performed First, all the sensors were kept at room temperature during the exposures Second, the heated sensors were heated to the first set of temperature values, TEMPI (The specific temperature values are listed m Tables 6 and 7 below ) This was followed by another exposure set where all the sensors were at room temperature Fourth, the second temperature experiment was repeated, TEMP2 The temperatures of TEMPI and of TEMP2 were meant to be nominally the same values (see data tables below) The fifth experiment was another set of room temperature exposures Finally, another heated exposure set, TEMP3, was collected

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The temperatures of the sensors m TEMP3 were adjusted to be higher than those in TEMPI or ΊΈΜΡ2

The average resistance and the standard deviations for the three trials in each 5 experiment were calculated and are listed in Table 6 Since the over-all signal for each sensor decreases at higher temperatures, the average responses and the standard deviation for each sensor were normalized 'and. are listed in

I ”

Table 7 The first, second, and third columns of the tables are the three room temperature experiments The last three columns are TEMPI, TEMP2, and TEMP3, respectively The values in Tables 6 and 7 are also presented graphically in Figures 11 and 12

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TABLE-6

<td></td><td></td><td> Exp l</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td colspan="2"> Exp 4 Exp 6</td>

<td> 5</td><td> AVERAGES</td><td> Sensor 3</td><td colspan="4"> poly(ethylene-vmyl acetate) (not heated)</td><td></td>

<td></td><td></td><td> 221C</td><td> 22 °C</td><td> 2TC</td><td> 22°C</td><td> 22°C</td><td> 22X</td>

<td></td><td> 2-propanol</td><td> 1 81E-O3</td><td> 1 75E-03</td><td> 1 91EO3</td><td> 1 84E-03</td><td> 1 94E-03</td><td> 1 84E-03</td>

<td></td><td> benzene</td><td> 6 25E-O3</td><td> 6 07E-03</td><td> 6 52E-03</td><td> 6 54E-O3</td><td> 6 49E-03</td><td> 6 19E-O3</td>

<td></td><td> chloroform</td><td> 5 67E-O3</td><td> 5 36E-03</td><td> 5 92E-03</td><td> 5 96E-03</td><td> 5 81E-03</td><td> 5 57EO3</td>

<td> 10</td><td> cyclohexane</td><td> 5 08E-03</td><td> 4 89E-O3</td><td> 5 35E-03</td><td> 5 17E-03</td><td> 5 27E-03</td><td> 5 06EO3</td>

<td></td><td> ethanol</td><td> 1 33E-03</td><td> 1 31E-03</td><td> 1 40E-03</td><td> 1 40E-03</td><td> 1 39E-03</td><td> 1 33ΕΌ3</td>

<td></td><td> hexane</td><td> 2 95E-03</td><td> 2 67E-03</td><td> 2 90E03</td><td> 3 04E-03</td><td> 2 93E-03</td><td> 2 7SE-O3</td>

<td></td><td> methanol</td><td> 3 31E-04</td><td> 3 21E-04</td><td> 3 53E-04</td><td> 4 27E-O4</td><td> 3 76E-04</td><td> 3 24E-O4</td>

<td></td><td> toluene</td><td> 2 59E 02</td><td> 2 51E-02</td><td> 2 69E-02</td><td> 2 63E-02</td><td> 2 68E-02</td><td> 2 50E-02</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td colspan="5"> Sensor 3 poly(cthylene-vmyl acetate) (not heated)</td>

<td></td><td></td><td> 22° C</td><td> 22 °C</td><td> 23°C</td><td> 22 °C</td><td> 2TC</td><td> 22°C</td>

<td> 20</td><td> 2 propanol</td><td> 1 31E-O4</td><td> 3 54E-05</td><td> 1 73E 05</td><td> 1 15E-04</td><td> 4 06E05</td><td> 1 32E04</td>

<td></td><td> benzene</td><td> 2 10E-04</td><td> 1 09E-C4</td><td> 7 78E 05</td><td> 1 07E-O4</td><td> 5 47E 05</td><td> 2 40E-04</td>

<td></td><td> chloroform</td><td> 2 68E-04</td><td> t 92E-04</td><td> 1 O8E-O4</td><td> 6 68E-O5</td><td> 1 54E-04</td><td> 4 12E-O4</td>

<td></td><td> c) clohexane</td><td> 3 4OE-04</td><td> 8 72E 05</td><td> 3 93E-05</td><td> 6 75E-O5</td><td> 1 04E-04</td><td> 1 27E-O4</td>

<td></td><td> ethanol</td><td> 1 04E-04</td><td> 4 64E06</td><td> 4 02E 05</td><td> 4 93E-O5</td><td> 6 15E-06</td><td> 9 95E-05</td>

<td> 25</td><td> hexane</td><td> 7 78E-05</td><td> 9 45E-05</td><td> 9 02E 05</td><td> 1 65E-O4</td><td> 4 74E-05</td><td> 1 27E-04</td>

<td></td><td> methanol</td><td> 4 35E-05</td><td> 8 52E-06</td><td> 3 66E-05</td><td> 1 13E-O4</td><td> 2 92E 05</td><td> 7 6OE-O5</td>

<td></td><td> toluene</td><td> 7 89E-O4</td><td> 1 09E04</td><td> 1 97E-04</td><td> 2 95E-O4</td><td> 7 18E-05</td><td> 6 94E-04</td>

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TABLE 6. cont.

<td></td><td> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> AVERAGES</td><td> Sensor 8</td><td colspan="4"> poly(ethylene-vinyl acetate) (not heated)</td><td></td>

<td></td><td> 22-C</td><td> 22°C</td><td> 23°C</td><td> 22X</td><td> 22X</td><td> 22°C</td>

<td> 2-propano)</td><td> 1 98E-03</td><td> 1 99E-O3</td><td> 2 09E-03</td><td> 1 99E-O3</td><td> 2 15E-O3</td><td> 2 07E-03</td>

<td> benzene</td><td> 6 30E-03</td><td> 6 29E-O3</td><td> 6 64E-03</td><td> 6 54E-O3</td><td> 6 65E-03</td><td> 6 40E-03</td>

<td> chlorofonn</td><td> 5 77E-03</td><td> 5 68E-O3</td><td> 6 07E-03</td><td> 6 05E 03</td><td> 6 06E-03</td><td> 5 89E-03</td>

<td> cyclohexane</td><td> 5 09E-03</td><td> 4 98E-O3</td><td> 5 33E-03</td><td> 5.20E-03</td><td> 5 29E-03</td><td> 5 10E-03</td>

<td> ethanol</td><td> 1 46E-03</td><td> I 48E-O3</td><td> 1 52E 03</td><td> 1 51E-03</td><td> 1 54E-O3</td><td> 1 51E-03</td>

<td> hexane</td><td> 2 89E-03</td><td> 2 71E-O3</td><td> 2 92E 03</td><td> 3 01E-03</td><td> 2 93E-03</td><td> 2 76E-O3</td>

<td> methanol</td><td> 4 09E-04</td><td> 3 81E-O4</td><td> 4 24E-04</td><td> 4 O8E-O4</td><td> 4 39Ε-Ό4</td><td> 4 05E-O4</td>

<td> toluene</td><td> 2 60E-02</td><td> 2 59E-O2</td><td> 2 70E-02</td><td> 2 63E-02</td><td> 2 74E 02</td><td> 2 59E-O2</td>

STANDARD DEVIATION Sensor 8 poly(ethylene-vmyl acetate) (not heated)

<td></td><td> 22 °C</td><td> 22X</td><td> 21LC</td><td> 22°C</td><td> 22X</td><td> 221S.</td>

<td> 2-propanol</td><td> 1 5 IE-04</td><td> 2 74E-05</td><td> 1 64E05</td><td> i 01E-04</td><td> 2 63E-05</td><td> I 54E-O4</td>

<td> benzene</td><td> 2 16E 04</td><td> 8 58E-O5</td><td> 1 10E-04</td><td> 1 35E 05</td><td> 4 36E-O5</td><td> 3 07E-04</td>

<td> chloroform</td><td> 3 16E-04</td><td> 1 85E-O4</td><td> 1 32E04</td><td> 1 25E 04</td><td> 2 21E-04</td><td> 4 06E-04</td>

<td> cyclohexane</td><td> 2 92E-04</td><td> 5 7ΊΕ-Ο5</td><td> 6 18E-O5</td><td> 6 35E 05</td><td> I 42E-04</td><td> I 59E-04</td>

<td> ethanol</td><td> 1 06E-04</td><td> 5 89E06</td><td> 2 59E-O5</td><td> 7 20E-05</td><td> 1 26E-05</td><td> 1 16E-04</td>

<td> hexane</td><td> 1 22E 05</td><td> 1 35E-04</td><td> 9 43E-O5</td><td> 7 96E-05</td><td> 6 86E-O5</td><td> 1 18E-O4</td>

<td> methanol</td><td> 5 87E-05</td><td> 3 07E-05</td><td> 4 54E-05</td><td> 5 25E-05</td><td> 4 22E-05</td><td> 8 63E 05</td>

<td> toluene</td><td> 8 10E-04</td><td> 1 75E-04</td><td> 2 71E-O4</td><td> 2 82E-04</td><td> I 08E-04</td><td> 7 10E-O4</td>

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TABLE 6. cnnt.

<td></td><td> AVERAGES</td><td> Exp 1 Sensor 17</td><td> Exp 3 poly((</td><td> Exp 5 :ttylene-vinyl</td><td> Exp 2 acetate)</td><td> Exp 4</td><td> Exp 6</td>

<td> 5</td><td></td><td> 22°C</td><td> 2ΤΏ</td><td> 2TC</td><td></td><td></td><td> 55°C</td>

<td></td><td> 2-propanol</td><td> 2 21E-03</td><td> 2 4IE-O3</td><td> 2 44E-03</td><td> 2 98E-04</td><td> 7 14E-04</td><td> 2 53E-04</td>

<td></td><td> benzene</td><td> 6 90E-03</td><td> 7 49E-03</td><td> 7 72E 03</td><td> 1 21EO3</td><td> 1 73E-O3</td><td> 7 61E-04</td>

<td></td><td> chloroform</td><td> 6 49E-03</td><td> 6 83E-03</td><td> 7 O7E-O3</td><td> 1 59E-03</td><td> 1 77EO3</td><td> 6 25E-04</td>

<td></td><td> cyclohexane</td><td> 5 80E-03</td><td> 6 2OE-O3</td><td> 6 41E-03</td><td> 1 97E-O3</td><td> 2 32E-O3</td><td> 9 23E-O4</td>

<td> 10</td><td> ethanol</td><td> 1 54EO3</td><td> 1 74ΕΌ3</td><td> 172E-03</td><td> 3 31E-04</td><td> 5 19E-04</td><td> 1 75E-O4</td>

<td></td><td> hexane</td><td> 3 33E-03</td><td> 3 34E-03</td><td> 3 46E-03</td><td> 1 29E-O3</td><td> 1 18E-O3</td><td> 7 20E-04</td>

<td></td><td> methanol</td><td> 4 I9E-O4</td><td> 4 45E-04</td><td> 4 55E-04</td><td> 3 68E-05</td><td> 5 85E-O4</td><td> 8 80E-05</td>

<td></td><td> toluene</td><td> 2 72E-O2</td><td> 2 93E-O2</td><td> 2 98E-02</td><td> 7 8IE-03</td><td> 6 29E-03</td><td> 3 63EO3</td>

<td rowspan="2"></td><td colspan="2"> STANDARD DEVIATION</td><td colspan="5"> Sensor 17 poly(ethylene-vtnyl acetate)</td>

<td> 2-propanol</td><td> 22££ 1 90E-04</td><td> 2TC 6 46E-O5</td><td> 22X 2 O5E-O5</td><td> 45X 4 27E-04</td><td> 46°C 2 19E-04</td><td> 551C 1 36E04</td>

<td> 20</td><td> benzene</td><td> 3 13E-04</td><td> 1 I3E-O4</td><td> I 38E-O4</td><td> I 30E 03</td><td> 4 55E-04</td><td> 1 51E-04</td>

<td></td><td> chloroform</td><td> 3 59E-O4</td><td> 2 87E-O4</td><td> 1 57E-O4</td><td> 1 75E-O4</td><td> 1 92E-04</td><td> 1 77E-04</td>

<td></td><td> c&gt;clohexanc</td><td> 4 04E-04</td><td> 1 79E-O4</td><td> 8 12E-05</td><td> 3 61E-O6</td><td> 3 20E-04</td><td> 1 51E-04</td>

<td></td><td> ethanol</td><td> 1 32E 04</td><td> 4 O9E-O5</td><td> 3 11E-O5</td><td> 1 22E-04</td><td> 1 08E-04</td><td> 1 HE-04</td>

<td></td><td> hexane</td><td> 8 52E 05</td><td> 1 86E-04</td><td> 9 90E-05</td><td> 2 44E-04</td><td> 1 37E-O5</td><td> 3 93E-04</td>

<td> 25</td><td> methanol</td><td> 5 97E 05</td><td> 3 50E-05</td><td> 5 45E 05</td><td> 9 24E-05</td><td> 8 97E-04</td><td> I 46E-04</td>

<td></td><td> toluene</td><td> 9 38E-04</td><td> 3 33E-04</td><td> 3 38EO4</td><td> 3 07E 03</td><td> 2 14E-O4</td><td> 2 95E-03</td>

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TABLE 6. cont.

<td></td><td> AVERAGES</td><td> Expl Sensor 20</td><td> Exp 3 poly(e</td><td> Exp 5 thylcne-vinyl</td><td> Exp 2 acetate)</td><td> Exp 4</td><td> Exp 6</td>

<td> 5</td><td></td><td> 22X</td><td> 22X</td><td> 23°C</td><td> 33°C</td><td> 242C</td><td> 2Z1£</td>

<td></td><td> 2-propanol</td><td> 3 83E-O3</td><td> 3 75E-03</td><td> 3 91E 03</td><td> 2 56E-03</td><td> 2 89E-03</td><td> 2 30E-03</td>

<td></td><td> benzene</td><td> 1 09E-02</td><td> I 09E-02</td><td> 1 12E-02</td><td> 8 36EO3</td><td> 8 20E-03</td><td> 6 82EO3</td>

<td></td><td> chloroform</td><td> 1 06E-02</td><td> 1 02E-02</td><td> 1 07E-02</td><td> 7 63E-03</td><td> 7 61E-03</td><td> 5 87E-03</td>

<td></td><td> cyclohexane</td><td> 9 50E-03</td><td> 9 22E-03</td><td> 9 63E-O3</td><td> 7 39E-03</td><td> 7 52E-03</td><td> 6 08E-03</td>

<td> 10</td><td> ethanol</td><td> 2 75E-03</td><td> 2 82E-03</td><td> 2 77E-O3</td><td> 2 00E-03</td><td> 2 43E-03</td><td> 1 82E-03</td>

<td></td><td> hexane</td><td> 5 39E-O3</td><td> 4 93EO3</td><td> 5 20E-03</td><td> 4 20E-03</td><td> 4 34E-03</td><td> 3 13E-O3</td>

<td></td><td> methanol</td><td> 8 33E-04</td><td> 7 91E-04</td><td> 8 08E-04</td><td> 6 09E-04</td><td> 9 35E-04</td><td> 5 63E-04</td>

<td></td><td> toluene</td><td> 4 49E-O2</td><td> 4 37EO2</td><td> 4 56E-02</td><td> 3 O1E-O2</td><td> 3 14E-02</td><td> 2 35E-02</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td colspan="4"> Sensor 20 poly(ethylcne-vmyl acetate)</td><td></td>

<td></td><td></td><td> 221C</td><td> 22£</td><td> 23°C</td><td> 33°C</td><td> 24ΣΏ</td><td></td>

<td></td><td> 2 propanol</td><td> 2 89EO4</td><td> 6 98E-05</td><td> 3 96E-05</td><td> 2 04E-04</td><td> 150E-04</td><td> 5 24E-04</td>

<td> 20</td><td> benzene</td><td> 4 7CE-04</td><td> 1 63E-04</td><td> 2 44E-04</td><td> 6 45E 04</td><td> 4 10E-04</td><td> 5.20E-04</td>

<td></td><td> chloroform</td><td> 6 0IEO4</td><td> 3 53E-04</td><td> 2 61E-04</td><td> 2 21E-04</td><td> 3 29E-04</td><td> 4 52E-04</td>

<td></td><td> cjclohexane</td><td> 6 43E-04</td><td> 1 88E-04</td><td> 1 46E 04</td><td> 3 20E-04</td><td> 8 44EO5</td><td> 6 69EO4</td>

<td></td><td> ethanol</td><td> 2 17E-04</td><td> I 31E-05</td><td> 5 29E 05</td><td> 9 05E-05</td><td> 3 28E-O4</td><td> 3 89E-04</td>

<td></td><td> hexane</td><td> I 7IE-04</td><td> 2 28E-O4</td><td> 1 38E04</td><td> 1 31E-04</td><td> 1 42E-04</td><td> 3 19E04</td>

<td> 25</td><td> methanol</td><td> 5 76E-05</td><td> 5 00E-05</td><td> 2 52E-05</td><td> 9 05E-05</td><td> 9 12E-04</td><td> 3 1IE-04</td>

<td></td><td> toluene</td><td> 1 75E-03</td><td> 3 75E-04</td><td> 5 35F04</td><td> I 71E-03</td><td> 5 16E-04</td><td> 9 34E-04</td>

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TABLE 6. cont.

<td></td><td></td><td> Expl</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td></td><td> AVERAGES</td><td> Sensor 4</td><td colspan="3"> polyethylene oxide) (not heated)</td><td></td><td></td>

<td> 5</td><td></td><td> 22X</td><td> 2T£</td><td> 23°C</td><td> 2TC</td><td> 22X</td><td> 22 °C</td>

<td></td><td> 2-propanol</td><td> 3 34E-03</td><td> 3 16E-O3</td><td> 3 34ΕΟ3</td><td> 3 33E-O3</td><td> 3 2BE-O3</td><td> 3 16E-O3</td>

<td></td><td> benzene</td><td> 8 I8E-03</td><td> 7 34E-O3</td><td> 8O6EO3</td><td> 8 25E-03</td><td> 7 86E-O3</td><td> 7 26E-O3</td>

<td></td><td> chloroform</td><td> 7 83EO3</td><td> 7 17E-03</td><td> 7 73E-03</td><td> 8 01E-03</td><td> 7 56E-O3</td><td> 7 2OE-O3</td>

<td></td><td> cyclohexane</td><td> 9 32E04</td><td> 8 13E-O4</td><td> 9 19E04</td><td> 9 16E-04</td><td> 8 74E-O4</td><td> 8 17E-O4</td>

<td> 10</td><td> ethanol</td><td> 3 12E-03</td><td> 2 94Ε-Ο3</td><td> 3 12E-03</td><td> 3 09E-O3</td><td> 3 05E-03</td><td> 2 90E-03</td>

<td></td><td> hexane</td><td> 7 00E-04</td><td> 5 70E-04</td><td> 6 80E-04</td><td> 6 85E-04</td><td> 6 47E-O4</td><td> 6 05E-04</td>

<td></td><td> methanol</td><td> 1 48E-03</td><td> 1 38E-O3</td><td> 1 48E 03</td><td> 1 47E-03</td><td> 1 44E-03</td><td> 1 39E-O3</td>

<td></td><td> toluene</td><td> 2 96E 02</td><td> 2 65E-O2</td><td> 2 88E-02</td><td> 2 93E-O2</td><td> 2 83E-O2</td><td> 2 62E-O2</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td colspan="5"> Sensor 4 polyethylene oxide) (not heated)</td>

<td></td><td></td><td> 22°C</td><td> 22°C</td><td></td><td> 22X</td><td> 2TC</td><td> 22°C</td>

<td></td><td> 2 propanol</td><td> 1 26E04</td><td> 6 19E-05</td><td> 3 44E 05</td><td> 1 13E-05</td><td> 6 71E-O6</td><td> 1 12E-O4</td>

<td> 20</td><td> benzene</td><td> 2 33E04</td><td> 2 30E-04</td><td> 7 16E-05</td><td> 1 07E-05</td><td> 6 90E-05</td><td> 1 74E-O4</td>

<td></td><td> chloroform</td><td> 3 43E-04</td><td> 3 64E-04</td><td> 1 47E-04</td><td> 1 26E-04</td><td> 2 29E-04</td><td> 2 85E-O4</td>

<td></td><td> cyclohexane</td><td> 1 72E 05</td><td> 5 23EO5</td><td> 1 83E 07</td><td> 8 32E-06</td><td> 1 81EO5</td><td> 1 76E-O5</td>

<td></td><td> ethanol</td><td> 1 40E-04</td><td> 1 37E-O5</td><td> 2 17E-05</td><td> 2 32E-O5</td><td> 2 82E-O5</td><td> 1 33E-O4</td>

<td></td><td> hexane</td><td> 1 37E05</td><td> 6 93E-O5</td><td> 3 62EQ6</td><td> 3 07E-06</td><td> 1 41EO5</td><td> 2O7E-O5</td>

<td> 25</td><td> methanol</td><td> 2 24E-05</td><td> 2 22E-O5</td><td> 2 5IE-05</td><td> 2 03E-05</td><td> 1 21E05</td><td> 449EO5</td>

<td></td><td> toluene</td><td> 4 39E-04</td><td> 1 17E-O4</td><td> 1 75E-O4</td><td> 2 24E-04</td><td> 8 57E-O5</td><td> 3 94E-O4</td>

Printed’from tomosa 03/10/1999 16 43 19 page -47WO 98/07024

-46PCT/US97/14070

TABLE 6. cont.

<td></td><td> Exp l</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> AVERAGES</td><td> Sensor 6</td><td colspan="3"> polyethylene oxide) (not heated)</td><td></td><td></td>

<td></td><td> 22°C</td><td> 22 °C</td><td> 23°C</td><td> 22*C</td><td> 22°C</td><td> 22X</td>

<td> 2-propanol</td><td> 3 30E-03</td><td> 3 08E-03</td><td> 3 29E-03</td><td> 3 27E-03</td><td> 3 21E-03</td><td> 3 11E-Q3</td>

<td> benzene</td><td> 9 14E-O3</td><td> 7 90E-03</td><td> 8 92E-03</td><td> 9 15E-O3</td><td> 8 61E-O3</td><td> 8 01E-03</td>

<td> chloroform</td><td> ' 8 33E-03</td><td> 7 45E-03</td><td> 8 19E-03</td><td> 8 42E-03</td><td> 7 90E-03</td><td> 7 58E-03</td>

<td> cyclohexane</td><td> 1 05E-03</td><td> 8 6OE-O4</td><td> 1 02E-03</td><td> 1 01E-03</td><td> 9 59E-04</td><td> 9 11E-04</td>

<td> ethanol</td><td> 2 97E-03</td><td> 2 78EO3</td><td> 2 97E-03</td><td> 2 94E-03</td><td> 2 90E-03</td><td> 2 78E-03</td>

<td> hexane</td><td> 8 32E-04</td><td> 6 13E-O4</td><td> 7 86E-04</td><td> 7 95E-04</td><td> 7 27E-O4</td><td> 7 00Ξ-04</td>

<td> methanol</td><td> 1 41E-03</td><td> 1 29E-03</td><td> 1 41E-O3</td><td> 1 4OE-O3</td><td> 1 36E-O3</td><td> 1 32E-03</td>

<td> toluene</td><td> 3 26E-02</td><td> 2 86E-02</td><td> 3 16E-O2</td><td> 3 21E-O2</td><td> 3 08E02</td><td> 2 86EO2</td>

STANDARD DEVIATION Sensor 6 polyethylene oxide) (not heated)

<td></td><td> 22 °C</td><td> 2T£</td><td> 23°C</td><td> 22° C</td><td> 22°C</td><td> 22°C</td>

<td> 2 propanol</td><td> 1 41E-04</td><td> 9 26E-05</td><td> 3 57E 05</td><td> 2O4EO5</td><td> 1 5OE-O5</td><td> 1 28E-04</td>

<td> benzene</td><td> 2 67E-04</td><td> 4 58E-04</td><td> 7 88E-05</td><td> 1 53E-05</td><td> 9 19E-O5</td><td> 2 07E-04</td>

<td> chloroform</td><td> 3 71E-04</td><td> 4 83E-O4</td><td> 1 55E-O4</td><td> 1 30E-04</td><td> 2 75E-04</td><td> 3 12E-04</td>

<td> c)clohexanc</td><td> 2 33EO5</td><td> 9 08E-05</td><td> 7 15E 06</td><td> 1 49Ε-05</td><td> 2 55E 05</td><td> 2 3OE-O5</td>

<td> ethanol</td><td> 1 33E-04</td><td> 4 14E-05</td><td> 2 35EO5</td><td> 1 28E-05</td><td> 1 62E-O5</td><td> 1 31E-04</td>

<td> hexane</td><td> 5 79E-06</td><td> l 20E-04</td><td> 8 29E-O6</td><td> 6 28E-06</td><td> l 99E-O5</td><td> 1 83EO5</td>

<td> methanol</td><td> 2 66EO5</td><td> 3 85EO5</td><td> 2 54E-O5</td><td> 1 50ΕΌ5</td><td> 1 27E-05</td><td> 5 24E-05</td>

<td> toluene</td><td> 4 69E 04</td><td> 4 37E-04</td><td> 1 57E-O4</td><td> 2 68E-04</td><td> 1 32E-04</td><td> 4 26EO4</td>

Printed from Mimosa 03/10/1999 16 43 19 page -48WO 58707024

-47PCT/U597/14070

TABLE 6. cont.

<td></td><td> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> AVERAGES</td><td> Sensor 9</td><td colspan="3"> polyethylene oxide) (not heated)</td><td></td><td></td>

<td></td><td> 222^</td><td></td><td> 22X</td><td> 22ΣΕ</td><td> 22*C</td><td> 221C</td>

<td> 2-propanol</td><td> 3 22E-03</td><td> 3 50E-03</td><td> 3 3OE-O3</td><td> 3 27EO3</td><td> 3 17E-03</td><td> 3 44E-03</td>

<td> benzene</td><td> 6 27EO3</td><td> 5 77ΕΌ3</td><td> 6 10E-03</td><td> 6 32EO3</td><td> 5 80E-03</td><td> 5 19E-O3</td>

<td> chloroform</td><td> 6 60E-03</td><td> 6 27E-03</td><td> 6 51E-O3</td><td> 6 78E-O3</td><td> 6 37E-03</td><td> 5 S5E-O3</td>

<td> cyclohexane</td><td> 7 82E-04</td><td> 9 06E-04</td><td> 7 59E-O4</td><td> 7 55E-04</td><td> 7 16E-04</td><td> 6 71E-O4</td>

<td> ethanol</td><td> 3 03E-03</td><td> 3 22E-03</td><td> 2 98E-O3</td><td> 2 96E-03</td><td> 2 99EO3</td><td> 3 02E-03</td>

<td> hexane</td><td> 5 46E-04</td><td> 8 I2E-O4</td><td> 5 37E-O4</td><td> 5 84E-O4</td><td> 5 11E-O4</td><td> 5 61E-O4</td>

<td> methanol</td><td> 1 50E 03</td><td> 1 75E-03</td><td> 1 45E-O3</td><td> 1 S1E-03</td><td> 1 55E-O3</td><td> 1 63E-O3</td>

<td> toluene</td><td> 2 20E02</td><td> 1 84E-02</td><td> 2 11E-02</td><td> 2 18E-02</td><td> 1 99E-02</td><td> 1 72E-02</td>

<td colspan="2"> STANDARD DEVIATION</td><td rowspan="2"> Sensor 9 22°C</td><td colspan="4"> polyethylene oxide) (not heated)</td>

<td></td><td> 22°C</td><td> 23°C</td><td> 22‘C</td><td> 22ΣΩ</td><td> 221C</td>

<td> 2 propanol</td><td> 1 61E-O4</td><td> 1 51E-O4</td><td> 7 00E-05</td><td> 2 98E-05</td><td> 1 I6E04</td><td> 3 78E-O4</td>

<td> benzene</td><td> 2 27E-O4</td><td> 4 88E-O4</td><td> 1 35E-O4</td><td> 1 08E-04</td><td> 4 O8E-O5</td><td> 9 07E-05</td>

<td> cltloroform</td><td> 2 06E-04</td><td> 1 36EO4</td><td> 5 63E-05</td><td> 6 31EO5</td><td> 1 40E-04</td><td> 4 07E-O4</td>

<td> cyclohexane</td><td> 5 07E-05</td><td> 1 66EO4</td><td> 3 37E-05</td><td> 9 69E-O5</td><td> 9 88E-O6</td><td> 3 78E-05</td>

<td> ethanol</td><td> 1 73E04</td><td> 2 07E-04</td><td> 1 65E-05</td><td> 6 02E05</td><td> 1 96E-O5</td><td> 1 85E-O4</td>

<td> hexane</td><td> 8 37E 05</td><td> 2 83E-04</td><td> 4 77EO5</td><td> 4 83E-05</td><td> 1 68E-O4</td><td> 1 64E-O4</td>

<td> methanol</td><td> 7 56E-05</td><td> 1 00E-04</td><td> 2 88E-O5</td><td> 5 50E-05</td><td> 8 35E 05</td><td> 1 31E-O4</td>

<td> toluene</td><td> 4 26E-04</td><td> 3 61E-O4</td><td> 8 15E-05</td><td> 2 75E-04</td><td> 4 39E-O4</td><td> 3 32E-O4</td>

Printed from Mimosa 03/10/1999 16:43 19 page -49WO 98/07024

-48PCT/US97/14070

TABLE 6. cont.

<td></td><td> AVERAGES</td><td> Expl Sensor 15</td><td> Exp 3 poly(e</td><td> Exp 5 thylene oxide'</td><td> Exp 2 1</td><td> Exp 4</td><td> Exp 6</td>

<td> 5</td><td></td><td> 222C</td><td> 22 °C</td><td> 23 °C</td><td> 44°C</td><td> 42^</td><td> 57°C</td>

<td></td><td> 2-propanol</td><td> 2 77E-O3</td><td> 2 98E-O3</td><td> 2 72E-03</td><td> 7 13E434</td><td> 9 59E-O4</td><td> 3 19E-04</td>

<td></td><td> benzene</td><td> 2 84E-03</td><td> 3 10E-O3</td><td> 2 68E-03</td><td> 9 04E-04</td><td> 1 20E-03</td><td> 3.39E-04</td>

<td></td><td> chloroform</td><td> 3 58E-O3</td><td> 3 69E-03</td><td> 3 36E-03</td><td> 1 08E-03</td><td> 1 24E-03</td><td> 3 68E-04</td>

<td></td><td> cyclohexane</td><td> 5 61E-04</td><td> 8 60E-04</td><td> 5 85E-04</td><td> 1 34E-O4</td><td> 3 74E-O4</td><td> 1 79E-04</td>

<td> 10</td><td> ethanol</td><td> 2 57E-03</td><td> 2 79E-O3</td><td> 2 47E-03</td><td> 7 74E-04</td><td> 759E-04</td><td> 3 65E-O4</td>

<td></td><td> hexane</td><td> 4 32E-04</td><td> 6 88E-O4</td><td> 4 27E-04</td><td> 2 39E-O4</td><td> 2 71E-O4</td><td> 7 47E-05</td>

<td></td><td> methanol</td><td> 1 30E-03</td><td> 1 56E-O3</td><td> I 27E-03</td><td> 4 09E-O4</td><td> 4O7E-O4</td><td> 2 53E-O4</td>

<td></td><td> toluene</td><td> 9 45E-03</td><td> 8 67E-O3</td><td> 8 77E-03</td><td> 3 66E-O3</td><td> 3 18E-O3</td><td> 1 29E-03</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td colspan="4"> Sensor 15 poly (ethylene oxide)</td><td></td>

<td></td><td></td><td> 22°C</td><td> 22°C</td><td> 23°C</td><td> 44°C</td><td> 47°C</td><td> 57°C</td>

<td></td><td> 2 propanol</td><td> 7 33E-05</td><td> 3 80E-05</td><td> 3 75E-05</td><td> 2 S7E-O4</td><td> 6 46E-05</td><td> 9 75E-O5</td>

<td> 20</td><td> benzene</td><td> 2 31E05</td><td> 4 43E-04</td><td> 5 95E-05</td><td> 3 78E-O4</td><td> 1 52E-04</td><td> 2 56E-O4</td>

<td></td><td> chloroform</td><td> 1 11E-04</td><td> 1 88E-O4</td><td> 2 75E 05</td><td> I 65E-04</td><td> 9 19E-05</td><td> 1 11ΕΌ4</td>

<td></td><td> cj clohexane</td><td> 2 69E-05</td><td> 1 20E-04</td><td> 1 32E-O4</td><td> 4 88E-O5</td><td> 1 96E-O4</td><td> 2 41E-04</td>

<td></td><td> ethanol</td><td> 8 53E-05</td><td> 1 61E-04</td><td> 1 35E-O4</td><td> 8 05E-05</td><td> 9 10E-05</td><td> 2 14E-04</td>

<td></td><td> hexane</td><td> 8 14E-05</td><td> 9 59E-05</td><td> 1 24E 05</td><td> 2 91E-04</td><td> 1 21E-04</td><td> 3 69E-O5</td>

<td> 25</td><td> methanol</td><td> 7 68E-05</td><td> 3 61E-05</td><td> 1 24E-O4</td><td> 7 45E-05</td><td> 1 30E-04</td><td> 1 33E-O4</td>

<td></td><td> toluene</td><td> 1 23E-04</td><td> 3 63E-O4</td><td> 9 63E 05</td><td> 7 71E-O5</td><td> I 43E-04</td><td> 2 46E-O4</td>

Printed from Mimosa 03/10/1999 16 43 19 page -50WO 98/07024

-49PCT/US97/14070

TABLE 6. cont.

<td></td><td> AVERAGES</td><td> Exp l Sensor 16</td><td> Exp 3 poly(e</td><td> Exp 5 thylene oxide]</td><td> Exp 2 1</td><td> Exp 4</td><td> Exp 6</td>

<td> 5</td><td></td><td> 22 °C</td><td> 22°C</td><td> 23°C</td><td> 23X</td><td></td><td></td>

<td></td><td> 2-propanol</td><td> 3 02E-03</td><td> 3 O3E-O3</td><td> 2 96E-03</td><td> 1 84E-O3</td><td> 1 81E-03</td><td> 1 35E-03</td>

<td></td><td> benzene</td><td> 6 36E-O3</td><td> 5 48E-03</td><td> 5 99EO3</td><td> 4 56E-O3</td><td> 4 I4E-03</td><td> 2 95E-03</td>

<td></td><td> chloroform</td><td> 6 49E-03</td><td> 5 86E-O3</td><td> 6 18E 03</td><td> 4 2OE-O3</td><td> 3 87EO3</td><td> 2 77E-03</td>

<td></td><td> cyclohexane</td><td> 6 70E-04</td><td> 6 68E-04</td><td> 6 34EO4</td><td> 5 19E-O4</td><td> 4 89E-O4</td><td> 4 13E-04</td>

<td> 10</td><td> ethanol</td><td> 2 74E-03</td><td> 2 81E-03</td><td> 2 68E-O3</td><td> 1 71EO3</td><td> 1 66E-O3</td><td> 1 28E-03</td>

<td></td><td> hexane</td><td> 4 22E-04</td><td> 5 13E-04</td><td> 4 37E-O4</td><td> 3 48E-O4</td><td> 3 53E-O4</td><td> 3 02E-04</td>

<td></td><td> methanol</td><td> 1 32E-03</td><td> 1 42E-03</td><td> I 27E-O3</td><td> 8 77E-O4</td><td> 8 50E-04</td><td> 7 23E-04</td>

<td></td><td> toluene</td><td> 2 28E-02</td><td> 1 87E-02</td><td> 2 08E-02</td><td> 1 61E-O2</td><td> 1 43E-02</td><td> 1 03E 02</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td colspan="4"> Sensor 16 polyethylene oxide)</td><td></td>

<td></td><td></td><td> 22X</td><td> 22°C</td><td> 23°C</td><td> 33 °C</td><td> 33 °C</td><td> 37°C</td>

<td></td><td> 2 propanol</td><td> 1 18E-04</td><td> 7 87 E 05</td><td> 2 67E-O5</td><td> 4 18E-05</td><td> 2 22EO5</td><td> 4 92EO5</td>

<td> 20</td><td> benzene</td><td> 2 12E-04</td><td> 1 65E-O4</td><td> 4 59E 05</td><td> 3 95E-O5</td><td> 6 30E-05</td><td> 1 12E-04</td>

<td></td><td> chloroform</td><td> 3 17E04</td><td> 8 02E-05</td><td> 1 20E-04</td><td> 4 67E-05</td><td> 6 31EO5</td><td> I 40E04</td>

<td></td><td> ctdohexane</td><td> 7 01E06</td><td> 7 16E 05</td><td> 8 73EO6</td><td> 4 19E-05</td><td> 4 15E-O5</td><td> 1 80E-05</td>

<td></td><td> ethanol</td><td> 1 27E-O4</td><td> 1 07E-O4</td><td> 1 53E 05</td><td> 2 90E-05</td><td> 1 12E-O5</td><td> 4 05ΕΌ5</td>

<td></td><td> hexane</td><td> 2 13E05</td><td> 7 84E-O5</td><td> 4 94E-06</td><td> 2 98E-C5</td><td> 2 28E 05</td><td> 3 25EO5</td>

<td> 25</td><td> methanol</td><td> 2 01E 05</td><td> 5 03E 05</td><td> 3 18E-05</td><td> 1 20E-05</td><td> 4 36E-05</td><td> 1 71EO5</td>

<td></td><td> toluene</td><td> 4 12E-04</td><td> 2 44E-O4</td><td> 1 78EO4</td><td> 2 16E-O4</td><td> 1 77E-O4</td><td> 1 18E04</td>

Printed from Mimosa 03/10/1999 16 43 19 page -51WO 98/07024

-50PCT/US97/14070

TABLE 6. cont.

<td></td><td> Expl</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> AVERAGES</td><td> Sensor 5</td><td colspan="3"> poly(4-vinyl phenol) (not heated)</td><td></td><td></td>

<td></td><td> 22°C</td><td> 22X</td><td> 22X</td><td> 22X</td><td> 22°C</td><td> 2TC</td>

<td> 2-propanol</td><td> 3 31E-O3</td><td> 1 60E-03</td><td> 2 56E-O3</td><td> 3 18E-O3</td><td> 2 44E-03</td><td> 1.53E-03</td>

<td> benzene</td><td> 5 11E-O4</td><td> 3 45E-04</td><td> 4 77E-O4</td><td> 5 23E-O4</td><td> 4 47EO4</td><td> 3 93E-04</td>

<td> chloroform</td><td> 9 93E-O4</td><td> 6 38E-04</td><td> 9 01E-04</td><td> 1 05E-03</td><td> 8 95E-04</td><td> 5 96E-O4</td>

<td> cyclohexane</td><td> 1 32EO4</td><td> 7 83E-O5</td><td> 9 96E 05</td><td> 7 61E-O5</td><td> 8 85E-O5</td><td> 1 01E-04</td>

<td> ethanol</td><td> 1 75E-02</td><td> 1 33E-O2</td><td> 1 57E-02</td><td> 1 67E-02</td><td> 1 51EO2</td><td> 1 27EO2</td>

<td> hexane</td><td> 1 33E-04</td><td> 1 05E-04</td><td> I20E-04</td><td> 1 74E-04</td><td> 1 10E-04</td><td> 1 07E-04</td>

<td> methanol</td><td> 1 27E-02</td><td> 1 15E-02</td><td> 1 18E-02</td><td> I 23E-02</td><td> 1 I8E-02</td><td> 1 1 IE-02</td>

<td> toluene</td><td> 1 08E-03</td><td> 7 02E-04</td><td> 9 74E-04</td><td> 1 O8E-O3</td><td> 8 69E-04</td><td> 6 69E-04</td>

STANDARD DEVIATION Sensor 5 poly(4-vinyl phenol) (not heated)

<td></td><td colspan="2"> 2T£</td><td> 22X</td><td> 2T£</td><td> 2T£</td><td> 22°C</td><td> 22‘C</td>

<td></td><td> 2-propanol</td><td> 1 26E-04</td><td> 7 17E-05</td><td> 5 29E-O5</td><td> 7 47E-05</td><td> 8 36E-05</td><td> 1 09E-04</td>

<td> 20</td><td> benzene</td><td> 1 84E-05</td><td> 2 92E 05</td><td> 4 24E-O5</td><td> 4 00E-05</td><td> 1 96E-O5</td><td> 2 42EO5</td>

<td></td><td> chloroform</td><td> 6 73E-05</td><td> 3 52E-05</td><td> 2 11E-05</td><td> 5 81E 05</td><td> 2 35E-O5</td><td> 4 43EO5</td>

<td></td><td> cyclohexane</td><td> 1 52E-O5</td><td> 3 30E-05</td><td> 1 33E-O5</td><td> 5 11E-05</td><td> 1 66E-05</td><td> 6 08E-O5</td>

<td></td><td> ethanol</td><td> 5 63E-04</td><td> 5 20E-04</td><td> 1 40E-O4</td><td> 5 19E-04</td><td> 3 34E-04</td><td> 6 10E-04</td>

<td></td><td> hexane</td><td> 3 54E-O5</td><td> 9 39E-O6</td><td> 2 96E-05</td><td> 3 I8E-O5</td><td> 3 84E-O6</td><td> 2 68E-05</td>

<td> 25</td><td> methanol</td><td> 5 31E-O5</td><td> 1 78E-04</td><td> 1 50E-O4</td><td> 1 62E-O4</td><td> 9 92E-05</td><td> 3 93EO4</td>

<td></td><td> toluene</td><td> 5 06E-05</td><td> 5OOE-O5</td><td> 9 14E-O5</td><td> 9 22E-O5</td><td> 4 81E-05</td><td> 5 81E-O6</td>

Printed from Mimosa 03/10/1999 16 43 19 page -52WO 98/07024

-51PCT/US97/14070

TABLE 6. cont.

<td></td><td> Expl</td><td> Exp 3</td><td> ExpS</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> AVERAGES</td><td> Sensor 7</td><td colspan="3"> poly(4-vmyl phenol) (not heated)</td><td></td><td></td>

<td></td><td> 22-C</td><td> 2T£</td><td> 23°C</td><td> 22X</td><td> 22°C</td><td> 221C</td>

<td> 2-propanol</td><td> 1 55E-O3</td><td> 2 49E-O4</td><td> 1 10E-03</td><td> 1 47E-03</td><td> 7 27E-O4</td><td> 2 63E-04</td>

<td> benzene</td><td> 6 95E-O4</td><td> 2 83E-O4</td><td> 4 63E-04</td><td> 4 46E-O4</td><td> 3 87E-04</td><td> 3O8E-O4</td>

<td> chloroform</td><td> 5 86E-O4</td><td> 2 19E-O4</td><td> 4 86E-04</td><td> 6 59E-O4</td><td> 3 87E-O4</td><td> 2 11E-04</td>

<td> cyclohexane</td><td> 3 35E-O4</td><td> 8O2E-O5</td><td> 1 17E-04</td><td> 1 61E-04</td><td> 8 9OE-O5</td><td> 1 43E-04</td>

<td> ethanol</td><td> 1 2OE-O2</td><td> 4 88E-O3</td><td> 1 06E-02</td><td> I 17E-02</td><td> 8 79E-O3</td><td> 4 38E-03</td>

<td> hexane</td><td> 1 84E-04</td><td> I 60E-04</td><td> 2 16E-04</td><td> 1 72E-04</td><td> 1 57F-O4</td><td> I 34E-04</td>

<td> methanol</td><td> 1 O9E-O2</td><td> 1 O2E-O2</td><td> 1 10E-02</td><td> 1 13E-O2</td><td> 1.29E-02</td><td> 1 04E-02</td>

<td> toluene</td><td> 1 06E-03</td><td> 6 43E-04</td><td> 9 00E-04</td><td> 9 33E-04</td><td> 7 33E-O4</td><td> 5 74E-04</td>

<td colspan="2"> STANDARD DEVIATION</td><td rowspan="2"> Sensor 7 22 °C</td><td rowspan="2"> P 23°C</td><td colspan="3"> ol/(4-vinyl phenol) (not heated)</td>

<td></td><td> 2? °C</td><td> 22X</td><td> 22X</td><td> 22°C</td>

<td> 2 propanol</td><td> 1 36E-O4</td><td> 4 15E-05</td><td> 8 21E-O5</td><td> 1 56E-O4</td><td> 5 48E-05</td><td> 5 45E-05</td>

<td> benzene</td><td> 1 16E-05</td><td> 8 23E-05</td><td> 8 53E-O5</td><td> 1 03E-04</td><td> 8 77E-Q5</td><td> 4 20E-05</td>

<td> chloroform</td><td> 6 41E-05</td><td> 1 21E-04</td><td> 7 59E-O5</td><td> 1 31E-O4</td><td> 1 36E-O4</td><td> 3 63E-O5</td>

<td> cyclohexane</td><td> 4 14E-05</td><td> 3 62E-O5</td><td> 5 71E-05</td><td> 6 57E-05</td><td> 3 SSE-05</td><td> 8 23E-05</td>

<td> ethanol</td><td> 8 41E-04</td><td> 4 75E-04</td><td> 2 43E04</td><td> 4 28E-O4</td><td> 5 34E-04</td><td> 2 41E-04</td>

<td> hexane</td><td> 1 12E-04</td><td> 5 94E-05</td><td> 9 81E-O5</td><td> 2 63E-O5</td><td> 4 34E-05</td><td> 6 98E-O5</td>

<td> methanol</td><td> 1 97E-O4</td><td> 5 53E-04</td><td> 2 48ΕΌ4</td><td> 3 93E-04</td><td> 5 12E-04</td><td> 2 80E-04</td>

<td> toluene</td><td> 8 16E-05</td><td> 8 98E-05</td><td> 9 25E-O5</td><td> 3 72E-05</td><td> 8 43E-05</td><td> 7 97E-05</td>

Printed from Mimosa 03/10/1999 16 43 19 page -53WO 98/07024

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TABLE.6. conL

<td></td><td> Expl</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> averages</td><td> Sensor 18</td><td colspan="3"> poly(4-vtnyl pnenol)</td><td></td><td></td>

<td></td><td> 22°C</td><td> 22°C</td><td> 2Z£</td><td> 4SX</td><td> 46°C</td><td> 64 °C</td>

<td> 2 propanol</td><td> 7 57E-O4</td><td> 1 57E-03</td><td> 9 23E-O4</td><td> 2 45E-03</td><td> 1 42E-O3</td><td> 4 89E-03</td>

<td> benzene</td><td> 358E-04</td><td> 2 9IE-03</td><td> 2 76E-03</td><td> 1 I2E-O3</td><td> 3 35E-O3</td><td> 2 62E-03</td>

<td> chloroform</td><td> 3 12E-03</td><td> 6 87E-03</td><td> 2 50E-03</td><td> 3 91E-03</td><td> 1 73E-O3</td><td> 4 88E-03</td>

<td> cyclohexane</td><td> 2 72E-03</td><td> 8 70E-03</td><td> 1 27E-03</td><td> 1 67E-O3</td><td> 2 42E-03</td><td> 3 45E-03</td>

<td> ethanol _ξ</td><td> 4 83E-03</td><td> 4 53E-O3</td><td> 5 14E-O3</td><td> 1 50E-02</td><td> 1 81E-03</td><td> 1 03E-02</td>

<td> hexane</td><td> 1 81E-03</td><td> 3 69E-03</td><td> 1.27E-03</td><td> 3 42E-03</td><td> 2 12E-03</td><td> 5 O6E-Q3</td>

<td> methanol</td><td> 3 18E 02</td><td> I 77E-02</td><td> 3 93E-02</td><td> 2 54E-O2</td><td> 3 42E-02</td><td> 1 20E-02</td>

<td> toluene</td><td> 3 89E-O3</td><td> 2 I7E-O3</td><td> 1 16E03</td><td> -9 19E-O4</td><td> 3 59E-03</td><td> 2 8IE-03</td>

<td colspan="2"> STANDARD DEVIATION</td><td colspan="4"> Sensor 18 poly(4-vinyl phenol)</td><td rowspan="2"> 64°C</td>

<td></td><td> 22°C</td><td> 2. Λ</td><td> 2Z£</td><td></td><td> 4£X</td>

<td> 2 propanol</td><td> 4 19E-O4</td><td> 2 50E-03</td><td> 4 77E-04</td><td> 9 88E-O4</td><td> 2 29E-03</td><td> 3 09E-03</td>

<td> benzene</td><td> 3 18E-03</td><td> 3 65E-03</td><td> 1 91E-O3</td><td> 2 53E-O3</td><td> 1 78E-04</td><td> 1 98E-03</td>

<td> chloroform</td><td> 2 20E-03</td><td> 3 96E-O4</td><td> 1 63E-03</td><td> 3 21E-O3</td><td> 1 08E-03</td><td> 7 30E-04</td>

<td> cyclohexane</td><td> 2 31E-03</td><td> 1 17E-O2</td><td> 1 56E-O3</td><td> 4 69E-03</td><td> 8 68E-O4</td><td> 1 7IE-03</td>

<td> ethanol</td><td> 1 61E-03</td><td> 2 37E-O3</td><td> 9 72E-O4</td><td> 2 33ΕΌ3</td><td> 2 40E-03</td><td> 9 56E-03</td>

<td> hexane</td><td> 1 01E-03</td><td> 2 80E 03</td><td> 6 31E-04</td><td> 1 70E-03</td><td> 8 44E-04</td><td> 1 95E-03</td>

<td> methanol</td><td> 8 72E-03</td><td> 5 25E-03</td><td> 1 23E-O3</td><td> 1 11E-02</td><td> 4 57E-O3</td><td> 5 97E-03</td>

<td> toluene</td><td> 3 90E-03</td><td> 5 32E-03</td><td> 9 34E-O4</td><td> 3 75E-03</td><td> 1 47E-03</td><td> 2 99E-O4</td>

Printed from Mimosa

Ο3Ί0/1999

43 19 page -54V

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<td></td><td> AVERAGES</td><td> Exp 1 Sensor 19</td><td> Exp 3 poly(4</td><td> Exp 5 kvinyl phenol]</td><td> Exp 2 1</td><td> Exp 4</td><td> Exp 6</td>

<td> 5</td><td></td><td> 221Ω</td><td> 22££</td><td> 22X</td><td> 37 °C</td><td> 38°C</td><td> 43°C</td>

<td></td><td> 2-propanol</td><td> 9 09E-04</td><td> 2 38E-04</td><td> 1 86E-04</td><td> 1 02E-03</td><td> 2 74E-04</td><td> 1 72E-04</td>

<td></td><td> benzene</td><td> 2 32E-04</td><td> 2 14E-04</td><td> 2 22E-04</td><td> 2 58E-O4</td><td> 1 52E-04</td><td> 1 28E-04</td>

<td></td><td> chloroform</td><td> 3 91E-04</td><td> 1 31E-04</td><td> 1 69E-04</td><td> 3 29E-O4</td><td> 1 76EO</td><td> 8 66E-05</td>

<td></td><td> cyclohexane</td><td> 1 31EO4</td><td> 8 34E-05</td><td> 1 16E-O4</td><td> 4 71E-O5</td><td> 7 94E-05</td><td> 1 25E-O4</td>

<td> 10</td><td> ethanol</td><td> 1 O9E-O2</td><td> 6 48E-03</td><td> 5 48EO3</td><td> 7 68E-O3</td><td> 4 81E-03</td><td> 2 38E-03</td>

<td></td><td> hexane</td><td> 8 28E-05</td><td> 9 80E-05</td><td> 1 O3E-O4</td><td> 4 48E-O5</td><td> 7 25E-05</td><td> 1 66E-O4</td>

<td></td><td> methanol</td><td> 1 06E-02</td><td> 9 9SEO3</td><td> 9 23E-03</td><td> f 73E-O3</td><td> 5 82E-O3</td><td> 4 04E-03</td>

<td></td><td> toluene</td><td> 4 57E-04</td><td> 4 I0E-O4</td><td> 4 62E-O4</td><td> 3 51EO4</td><td> 2 17E-04</td><td> 1 62E-O4</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td colspan="4"> Sensor 19 poly(4 vinyl phenol)</td><td></td>

<td></td><td></td><td> 22°C</td><td> 22X</td><td> 23°C</td><td> 37°C</td><td> 38°C</td><td> 43 °C</td>

<td></td><td> 2-propanol</td><td> 1 62E-05</td><td> 4 38E 05</td><td> 6 09E-05</td><td> 5 69E-O5</td><td> 1 25E-0-4</td><td> 8 61E-O5</td>

<td> 20</td><td> benzene</td><td> 1 60E-05</td><td> 7 93E 05</td><td> 6 85E-05</td><td> 1 43E-O4</td><td> 4 41E-05</td><td> 2 55E 05</td>

<td></td><td> chloroform</td><td> 6 82EO5</td><td> 2 86E-05</td><td> 1 82E-05</td><td> 2 95E-O5</td><td> 9 03E-05</td><td> 3 69E-O5</td>

<td></td><td> cyclohexane</td><td> 1 32EO5</td><td> 4 94E-05</td><td> 7 90E 05</td><td> 6 32E-O5</td><td> 3 30E-05</td><td> 3 08E-05</td>

<td></td><td> ethanol</td><td> 5 51E-04</td><td> 5 99E-O4</td><td> 3 17E-O5</td><td> 7 08E-04</td><td> 5 44E-04</td><td> 4 33E-O4</td>

<td></td><td> hexane</td><td> 4 84E-05</td><td> 6 92E-06</td><td> 3 30E-05</td><td> 9 91E-O5</td><td> 1 03E-05</td><td> 7 23EO5</td>

<td> 25</td><td> methanol</td><td> 2 2IE-04</td><td> 4 53EO4</td><td> I 04E-04</td><td> 5 95E-04</td><td> 2 2IE04</td><td> 3 94E-04</td>

<td></td><td> toluene</td><td> 9 02E 05</td><td> 2 26ΕΌ5</td><td> 1 O2E-O4</td><td> 1 68E-O5</td><td> 1 38E-O4</td><td> I 72EO4</td>

Printed from Mimosa 03/10/1999 16 43 19 page -5598/07024

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TABLE!

<td></td><td> Exp l</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> Normalized</td><td> Sensor 3</td><td colspan="4"> poly(ethylene-vtnyl acetate) (not heated)</td><td></td>

<td> averages</td><td> 22°C</td><td> 22X</td><td> 23°C</td><td> 22°C</td><td> 22X</td><td> 22°C</td>

<td> 2-propancl</td><td> 3 67E-02</td><td> 3 69E-02</td><td> 3 73E-02</td><td> 3 63E-02</td><td> 3 80E-02</td><td> 3 82E-02</td>

<td> benzene</td><td> I 27E-01</td><td> 1 28EO1</td><td> 1 27E-01</td><td> 1 29E-01</td><td> 1 27E 01</td><td> 1 29EO1</td>

<td> chloroform</td><td> 1 15E-01</td><td> 1 13E-01</td><td> 1 16E-01</td><td> 1 18E-01</td><td> 1 14E-01</td><td> 1 16E-O1</td>

<td> cyclohexane</td><td> 1 03EO1</td><td> 1 03E-01</td><td> 104E-01</td><td> 1 02E-01</td><td> 1 03E-01</td><td> 1 05E-01</td>

<td> ethanol</td><td> 2 70E-02</td><td> 2 76E-02</td><td> 2 73E-02</td><td> 2 77E-02</td><td> 2 73E-O2</td><td> 2 77E-O2</td>

<td> hexane</td><td> 5 97E-O2</td><td> 5 63E-02</td><td> 5 67E-02</td><td> S 99E-O2</td><td> 5 7SE 02</td><td> 5 72E-O2</td>

<td> methanol</td><td> 6 71E03</td><td> 6 76E-O3</td><td> 6 89E-O3</td><td> 8 42E-03</td><td> 7 37E-03</td><td> 6 74EO3</td>

<td> toluene</td><td> 5 25E-01</td><td> 5 28EO1</td><td> 5 25E 01</td><td> 5 I9E01</td><td> 5 25E-01</td><td> 5 21E-01</td>

<td> Normalized</td><td> Sensor 3</td><td colspan="4"> poly(ethylcne-vtnyl acetate) (not heated)</td><td></td>

<td colspan="2"> STANDARD DEVIATION</td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> 22°C</td><td> 22°C</td><td> 23°C</td><td> 22°C</td><td> 22X</td><td> 22°C</td>

<td> 2-propanol</td><td> 2 66E-O3</td><td> 7 46E-O4</td><td> 3 37E-04</td><td> 2 26EO3</td><td> 7 96E04</td><td> 2 75E-O3</td>

<td> benzene</td><td> 4 25E 03</td><td> 2 31E-O3</td><td> 1 52E-03</td><td> 2 12E-03</td><td> 1 07E 03</td><td> 4 98E-O3</td>

<td> chloroform</td><td> 5 44E-03</td><td> 4 04E-03</td><td> 2 12E-03</td><td> 1 32E03</td><td> 3 03E-03</td><td> 8 57E-O3</td>

<td> c&gt; clohexane</td><td> 6 89E-O3</td><td> 1 84EO3</td><td> 7 68E04</td><td> 1 33E-03</td><td> 2 04E-03</td><td> 2 64EO3</td>

<td> ethanol</td><td> 2 11E-03</td><td> 9 77E05</td><td> 7 85EO4</td><td> 9 72E-04</td><td> 1 21E-O4</td><td> 2 O7E-O3</td>

<td> hexane</td><td> 1 58E-O3</td><td> 1 99E 03</td><td> 1 76E 03</td><td> 3 26E-O3</td><td> 9 29E04</td><td> 2 63E-O3</td>

<td> methanol</td><td> 8 81E-O4</td><td> 1 80E-04</td><td> 7 15E-04</td><td> 2 22E-03</td><td> 5 73E04</td><td> 1 58EO3</td>

<td> toluene</td><td> 1 6OEO2</td><td> 2 29E-O3</td><td> 3 84E-03</td><td> 5 82E 03</td><td> 1 41E-03</td><td> 1 44E-02</td>

if

Printed from Mimosa 03/10/1999 16 43 19 page -56WO 98/07024

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TABLE 7. cont.

<td></td><td colspan="2"> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td></td><td> Normalized</td><td> Sensor 8</td><td colspan="4"> poly(eftylene-vtnyl acetate) (not heated)</td><td></td>

<td> 5</td><td> averages</td><td> 2T£</td><td> 221C</td><td> 23°C</td><td> 222Z</td><td> 22“C</td><td> 22°C</td>

<td></td><td> 2-propanol</td><td> 3 97E-02</td><td> 4 02E-02</td><td> 4 01E-O2</td><td> 3 9IE-O2</td><td> 4 I0E-02</td><td> 4 13E-O2</td>

<td></td><td> benzene</td><td> 1 26E-O1</td><td> 1 27E-01</td><td> 1 28E-01</td><td> 1 28E-01</td><td> 1 27E-01</td><td> 1 28E-01</td>

<td></td><td> chloroform</td><td> 1 16E-O1</td><td> 1 15E-O1</td><td> 1 17E-01</td><td> 1 19E-0I</td><td> 1 16EO1</td><td> 1 18E-01</td>

<td> 10</td><td> cyclohexane</td><td> 1 02E-OI</td><td> 1 01E-01</td><td> 1 02E-0I</td><td> 1 02E-O1</td><td> 101EO1</td><td> 1 02E-01</td>

<td></td><td> ethanol</td><td> 2 93E-02</td><td> 3 00E-02</td><td> 2 91E 02</td><td> 2 96E-02</td><td> 2 94E-02</td><td> 3 01E-02</td>

<td></td><td> hexane</td><td> 5 79E-02</td><td> 5 48E-02</td><td> 5 61E 02</td><td> 5 90E-02</td><td> 5 60E-02</td><td> 5553E-02</td>

<td></td><td> methanol</td><td> 8 20E-03</td><td> 7 71E-O3</td><td> 8 14E-O3</td><td> 8 01E-O3</td><td> 8 37E-03</td><td> 8 10E-03</td>

<td></td><td> toluene</td><td> 5 21E-01</td><td> 5 25EOI</td><td> 5 20E-0!</td><td> S 16E-O1</td><td> 5 22E-01</td><td> 5 17E-01</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> Normalized STANDARD I</td><td> Sensor 8 1EVIATION</td><td colspan="5"> poly(cthylene-vinyl acetate) (not heated)</td>

<td> 20</td><td></td><td> 22°C</td><td> 22X</td><td> 23°C</td><td> 2^£</td><td> 22°C</td><td> 22°C</td>

<td></td><td> 2 propanol</td><td> 3 O3E-O3</td><td> 534E-04</td><td> 3 15E-04</td><td> 1 98E-O3</td><td> 5 01E-04</td><td> 3 O8E-O3</td>

<td></td><td> benzene</td><td> 4 33E-O3</td><td> 1 74EO3</td><td> 2 12E-03</td><td> 2 65E-O4</td><td> 8 33EO4</td><td> 6 15E-03</td>

<td></td><td> chloroform</td><td> 6 34EO3</td><td> 3 74E-03</td><td> 2 54E-O3</td><td> 2 46E-O3</td><td> 4 21E 03</td><td> 8 12E-03</td>

<td></td><td> cyclohexane</td><td> 5 86E-03</td><td> 1 16E-O3</td><td> 1 19E-O3</td><td> 1 25E-O3</td><td> 2 70E 03</td><td> 3 18E-03</td>

<td> 25</td><td> ethanol</td><td> 2 13E-03</td><td> 1 19E-04</td><td> 4 98E04</td><td> 1 41E-03</td><td> 2 40E-04</td><td> 2 32EO3</td>

<td></td><td> hexane</td><td> 2 45E-04</td><td> 2 73E-03</td><td> I 81E-O3</td><td> 1 56E-O3</td><td> 1 3IE-03</td><td> 2 36E-03</td>

<td></td><td> methanol</td><td> 1 18E-03</td><td> 6 21EO4</td><td> 8 73E-04</td><td> 1 03E-03</td><td> 8 06E-04</td><td> 1 73E-O3</td>

<td></td><td> toluene -</td><td> t 62E-02</td><td> 3 54EO3</td><td> 5 2OE-O3</td><td> S 53E-O3</td><td> 2 06E-03</td><td> I 42E-02</td>

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TABLE 7. cont.

<td colspan="3"> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td></td><td> Normalized</td><td> Sensor 17</td><td colspan="3"> poly(ethylene-vinyl acetate)</td><td></td><td></td>

<td> 5</td><td> averages</td><td> 22*C</td><td> 22°C</td><td> 2IX</td><td></td><td> 46°C</td><td></td>

<td></td><td> 2 propanol</td><td> 4 10E-02</td><td> 4 17E-O2</td><td> 4 13E-02</td><td> 2 05E-02</td><td> 4 72E-02</td><td> 3 52EO2</td>

<td></td><td> benzene</td><td> 1 28E-01</td><td> 1 30E-01</td><td> 1 31E-01</td><td> 8 31E-02</td><td> 1 15E-01</td><td> 1 06E-01</td>

<td></td><td> chloroform</td><td> 1 20E-01</td><td> 1 18E-01</td><td> I 20E-01</td><td> 1 09E-01</td><td> 1 17E-01</td><td> 8 71EO2</td>

<td> 10</td><td> cyclohexane</td><td> 1 08E-0I</td><td> 1 07E-01</td><td> 1 08E 01</td><td> 1 35ΕΌ1</td><td> 1 54E-01</td><td> 1 29E-OI</td>

<td></td><td> ethanol</td><td> 2 86E-02</td><td> 3 Ο2Ε-Ό2</td><td> 2 91E-02</td><td> 2 28E-O2</td><td> 3 43E-O2</td><td> 2 43E-O2</td>

<td></td><td> hexane</td><td> 6 18E-O2</td><td> 5 79E-O2</td><td> 5 85E-02</td><td> 8 87E-O2</td><td> 7 79E-02</td><td> 1 00EO1</td>

<td></td><td> methanol</td><td> 7 78E-03</td><td> 7 71EO3</td><td> 7 70E-03</td><td> 2 53E03</td><td> 3 87E-O2</td><td> 1 23EO2</td>

<td></td><td> toluene</td><td> 5O4EO1</td><td> 5 07E-01</td><td> 5 05E-01</td><td> 5 37E01</td><td> 4 16E-01</td><td> 5 06E-01</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> Not malized</td><td> Sensor 17</td><td colspan="3"> poly(ethylene-vmyl acetate)</td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td></td><td></td><td></td><td></td><td></td>

<td> 20</td><td> 22°C</td><td> 22°C</td><td> 2T£</td><td></td><td> 4££</td><td> 55°C</td><td></td>

<td></td><td> 2 propanol</td><td> 3 52E 03</td><td> 1 12E-O3</td><td> 3 46E-04</td><td> 2 94EO2</td><td> 1 45E-02</td><td> I 90E-02</td>

<td></td><td> benzene</td><td> 5 81E-03</td><td> 1 97EO3</td><td> 2 33E 03</td><td> 8 94E-02</td><td> 3 01E-02</td><td> 2 11E-02</td>

<td></td><td> chloroform</td><td> 7 22E-03</td><td> 4 96E-O3</td><td> 2 66EO3</td><td> 1 20E-02</td><td> 1 27E-O2</td><td> 2 47E-02</td>

<td></td><td> cyclohexane</td><td> 7 49E-03</td><td> 3 11E-03</td><td> 1 37E 03</td><td> 2 48E04</td><td> 2 12E-02</td><td> 2 10E-02</td>

<td> 25</td><td> ethanol</td><td> 2 44EO3</td><td> 7 09E-04</td><td> 5 26E04</td><td> 8 4OE-O3</td><td> 7 16E-O3</td><td> 1 55E-02</td>

<td></td><td> hexane</td><td> 1 58EO3</td><td> 3 23E-O3</td><td> 1 67E-03</td><td> 1 68E-O2</td><td> 9O6EO4</td><td> 5 47EO2</td>

<td></td><td> methanol</td><td> 1 11E-03</td><td> 6 06E-04</td><td> 9 22E-04</td><td> 6 36E 03</td><td> 5 94E-02</td><td> 2 O4E-O2</td>

<td></td><td> toluene</td><td> 1 74E-02</td><td> 5 77E-03</td><td> 5 71E-03</td><td> 2 11E-OI</td><td> 1 42E-O2</td><td> 4 10E-01</td>

Printed from Mimosa 03/10/1999 16 43 19 page -58Exp 1 Exp 3 Exp 5 Exp 2

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-57TABLE 7. cont,

Exp

Exp 6

<td> 5</td><td> Normalized</td><td> Sensor 2(0</td><td colspan="3"> poly(elhylene-vinyl acetate)</td><td></td>

<td></td><td> averages</td><td> 22°C</td><td> 22 *C</td><td> 23X</td><td> 33 *C</td><td> 34 *C</td>

<td></td><td> 2-propanol</td><td> 4 31E-02</td><td> 4 35E-G2</td><td> 4 35E-02</td><td> 4 O8E-O2</td><td> 4 42E-02</td>

<td></td><td> benzene</td><td> 123E-01</td><td> 1 26EO1</td><td> 1 25EO1</td><td> 1 33E-01</td><td> 1 25E-01</td>

<td> 10</td><td> chloroform</td><td> 1 20E-01</td><td> 1 18E-01</td><td> 1 19E-01</td><td> 1 21E-01</td><td> 1.16E-01</td>

<td></td><td> cyclohexane</td><td> 1 07E-01</td><td> 1 07E-01</td><td> 1 07E-01</td><td> 1 18E-01</td><td> 1 15E01</td>

<td></td><td> ethanol</td><td> 3 10E-02</td><td> 3 27E-02</td><td> 3 08E-02</td><td> 3 18E-02</td><td> 3 73E-02</td>

<td></td><td> hexane</td><td> 6 07E-02</td><td> 5 72E-02</td><td> 5 79E-02</td><td> 6 68E-02</td><td> 6 64E-02</td>

<td></td><td> methanol</td><td> 9 39E-03</td><td> 9 17E-O3</td><td> 8 99E 03</td><td> 9 69E-O3</td><td> 1 43E-02</td>

<td> 15</td><td> toluene</td><td> 5 06E-01</td><td> 5 06E-01</td><td> 5 07E 01</td><td> 4 79E-01</td><td> 4 81E-01</td>

37°C 4 60E-02 1 36EO1 1 17E-01 1 21E-01

63E 02 6 26E-02 1 12E-02

69E-01

<td></td><td> Normalized</td><td> Sensor 20</td><td colspan="2"> poly(ethylene-vinyl</td><td colspan="2"> acetate)</td>

<td> 20</td><td colspan="2"> STANDARD DEVIATION 22'C</td><td> 22LC</td><td> 23 °C</td><td> 33“C</td><td> 2EC</td>

<td></td><td> 2 propanol</td><td> 3 26EO3</td><td> 8 09E 04</td><td> 4 41E-O4</td><td> 3 24E-03</td><td> 2 30E-03</td>

<td></td><td> benzene</td><td> 5 36E-O3</td><td> 1 89E-03</td><td> 2 72E-03</td><td> 1 03E-02</td><td> 6 27E-03</td>

<td></td><td> chloroform</td><td> 6 77E-O3</td><td> 4 09E-O3</td><td> 2 90E-03</td><td> 3 51E-03</td><td> 5 03E-03</td>

<td> 25</td><td> cyclohexane</td><td> 7 25E-O3</td><td> 2 18E-O3</td><td> 1 63E-O3</td><td> 5 10E-03</td><td> 1 29EO3</td>

<td></td><td> ethanol</td><td> 2 45E-03</td><td> 1 51E-O4</td><td> 5 88E-04</td><td> 1 4-4E-O3</td><td> 5 03E 03</td>

<td></td><td> hexane</td><td> 1 93EO3</td><td> 2 64E-O3</td><td> 1 54E-03</td><td> 2 09E-03</td><td> 2 V7E03</td>

<td></td><td> methanol</td><td> 6 49E-04</td><td> 5 80E-O4</td><td> 2 81E-O4</td><td> 1 44E-03</td><td> 1 40E02</td>

<td> 30</td><td> toluene</td><td> 1 98E-O2</td><td> 4 35E-03</td><td> 5 95E-O3</td><td> 2 72E-02</td><td> 7 90E03</td>

37*C 1 05E-02 1 04E-02 9 04E-03 1 34E-02 7 77EO3 6 38E-03 6 22E-03 1 86E-02

Printed from Mimosa 03/10/1999 16 43 19 page -59WO 98/07024

-58PCT/US97/14070

TABLE?, cont,

<td></td><td> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> Normalized</td><td> Sensor 4</td><td colspan="3"> polyethylene oxide) (not heated)</td><td></td><td></td>

<td> averages</td><td> 22°C</td><td> 22 °C</td><td> 23“C</td><td> 22 °C</td><td> 22°C</td><td></td>

<td> 2-propanol</td><td> 6O6E-O2</td><td> 6 34E-02</td><td> 6 16E-O2</td><td> 6 05E-02</td><td> 6 18E-02</td><td> 6 38EO2</td>

<td> benzene</td><td> 1 48E-01</td><td> 1 47EO1</td><td> t 49EO1</td><td> 1 50E-01</td><td> 1 48E-OI</td><td> 1 47E-01</td>

<td> chloroform</td><td> 1 42E-01</td><td> 1 44E-01</td><td> 1 43EO1</td><td> 1 46E-01</td><td> 1 43EO1</td><td> 1 45E-O1</td>

<td> cyclohexane</td><td> 1 69E-02</td><td> 1 63E-02</td><td> 1 70E-02</td><td> 1 66E-02</td><td> 1 65E-O2</td><td> 1 65E-02</td>

<td> ethanol</td><td> 5 66E-02</td><td> 5 89E-O2</td><td> 5 77E-O2</td><td> 5 62E-O2</td><td> 5 74E-02</td><td> 5 85E-02</td>

<td> hexane</td><td> 1 27E-02</td><td> 1 14E-02</td><td> 1 26E-O2</td><td> 1 25EO2</td><td> I 22E-02</td><td> 1 22E-02</td>

<td> methanol</td><td> 2 69E-O2</td><td> 2 76E-02</td><td> 2 74E-O2</td><td> 2 68E 02</td><td> 2 72E-O2</td><td> 2 80E-02</td>

<td> toluene</td><td> 5 36E01</td><td> 5 31E-01</td><td> 5 32E-01</td><td> 5 32EO1</td><td> 5 34E-01</td><td> 5 29E-01</td>

<td> Normalized</td><td> Sensor 4</td><td colspan="3"> pol) (ethylene oxide) (not heated)</td><td></td><td></td>

<td colspan="2"> STANDARD DEVIATION</td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> 22 °C</td><td> 22‘C</td><td></td><td> 22°C</td><td> 22°C</td><td></td>

<td> 2 propanol</td><td> 2 28EO3</td><td> 1 24E-03</td><td> 6 36E-O4</td><td> 2 06E04</td><td> 1 27E-04</td><td> 2 26E-O3</td>

<td> benzene</td><td> 4 22E-O3</td><td> 4 61E-O3</td><td> 1 32E 03</td><td> 1 94E-O4</td><td> 1 30E-03</td><td> 3 51E-O3</td>

<td> chloroform</td><td> 6 22E 03</td><td> 7 31EO3</td><td> 2 71EO3</td><td> 2 29E 03</td><td> 4 33E 03</td><td> 5 76EO3</td>

<td> cj clohexane</td><td> 3 11E-04</td><td> 1 05E-03</td><td> 3 37E-06</td><td> 1 51E-04</td><td> 3 40EO4</td><td> 3.55E-04</td>

<td> ethanol</td><td> 2 53E-03</td><td> 2 74EO4</td><td> 4 0IE-04</td><td> 4 21E-04</td><td> 5 31E-04</td><td> 2 68EO3</td>

<td> hexane</td><td> 2 48E-04</td><td> 1 39E 03</td><td> 6 69E OS</td><td> 5 57E-05</td><td> 2 66E-04</td><td> 4 18E-O4</td>

<td> methanol</td><td> 4 06E-04</td><td> 4 45E-04</td><td> 4 64E-O4</td><td> 3 70E04</td><td> 2 29E-O4</td><td> 9 06E-04</td>

<td> toluene</td><td> 7 97E-03</td><td> 2 34E-03</td><td> 3 23E 03</td><td> 4 07E-03</td><td> 1 62EO3</td><td> 7 97E 03</td>

Printed from Mimosa 03/10/1999 16 43 19 page -60WO 98/07024

-59PCT/US97/I4070

TABLE 7. cont.

<td></td><td> Exp 1</td><td> Exp 3</td><td> Exp S</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> Normalized</td><td> Sensor 6</td><td colspan="3"> polyethylene oxide) (not heated)</td><td></td><td></td>

<td> at crages</td><td> 22° C</td><td></td><td> 23°C</td><td> 22X</td><td> 222£</td><td> 22 °C</td>

<td> 2-propanol</td><td> 5 53E-O2</td><td> 5 86E-02</td><td> 5 65E 02</td><td> S 53E-O2</td><td> 5 69E-O2</td><td> 5 86E-O2</td>

<td> benzene</td><td> 1 53E-0I</td><td> 1 50E-01</td><td> 1 53E-O1</td><td> 1 5SE-OI</td><td> Ι.53ΕΌ1</td><td> 1 51E-O1</td>

<td> chloroform</td><td> 1 40E 01</td><td> 1 42E 01</td><td> 1 41ΕΌ1</td><td> 1 43E-O1</td><td> 1 40E-01</td><td> 1 43E-O1</td>

<td> cyclohexane</td><td> 1 75E-02</td><td> 1 63E-02</td><td> 1 76ΕΌ2</td><td> 1 72E-O2</td><td> 1 70E-02</td><td> 1 72E-O2</td>

<td> ethanol</td><td> 4 98E-O2</td><td> 5 28E-02</td><td> 5 10E 02</td><td> 4 97E-02</td><td> 5 14E-O2</td><td> 5 24E-O2</td>

<td> hexane</td><td> 1 39E-O2</td><td> 1 16E-02</td><td> 1 35E-O2</td><td> 1 35E-O2</td><td> 1 29E-02</td><td> 1 32E-02</td>

<td> methanol</td><td> 2 36E-02</td><td> 2 46E-02</td><td> 2 43E 02</td><td> 2 36E 02</td><td> 2 41E-02</td><td> 2 49E-O2</td>

<td> toluene</td><td> 5 47E Ol</td><td> 5 44E-OI</td><td> 5 43E01</td><td> 5 43E Ol</td><td> 5 45E-01</td><td> 5 40E-O1</td>

<td> Normalized</td><td> Sensor 6</td><td colspan="3"> poly(cthylene oxide) (not heated)</td><td></td><td></td>

<td colspan="2"> STANDARD DEVIATION</td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> 222C</td><td> 22°C</td><td> -</td><td> 22X</td><td> 22°C</td><td> 22° C</td>

<td> 2-propanol</td><td> 2 36E-03</td><td> 1 76E 03</td><td> ο I5E-O4</td><td> 3 46E-O4</td><td> 2 65E-04</td><td> 2 42E-03</td>

<td> benzene</td><td> 4 47E-03</td><td> 8 71F 0’</td><td> 1 36E-O3</td><td> 2 59E-O4</td><td> 1 63E-O3</td><td> 3 91E-O3</td>

<td> chloroform</td><td> 6 22E O’</td><td> -03</td><td> 2 66E 03</td><td> 2 20E-03</td><td> 4 88E-03</td><td> 5 88E-O3</td>

<td> c&gt; clohexane</td><td></td><td> 1 73E-03</td><td> 1 23E-O4</td><td> 2 53E-O4</td><td> 4 51E-04</td><td> 4 35E-04</td>

<td> ethanol</td><td> J3</td><td> 7 86E-04</td><td> 4 03E-O4</td><td> 2 16E-C4</td><td> 2 87E-04</td><td> 2 47E-O3</td>

<td> hexai</td><td> 9 70E 05</td><td> 2 27E-03</td><td> 1 43E-O4</td><td> 1 06E04</td><td> 3 53E04</td><td> 3 45E-O4</td>

<td> methanol</td><td> 4 47E-04</td><td> 7 32E-O4</td><td> 4 36E-O4</td><td> 2 54E-O4</td><td> 2 25E-04</td><td> 9 89E-O4</td>

<td> toluene</td><td> 7 87E 03</td><td> 8 31E-O3</td><td> 2 69E-O3</td><td> 4 53E-O3</td><td> 2 34E-03</td><td> 8 03E-03</td>

Printed from Mimosa 03/10/1999 16 43 19 page -61WO 98/07024

-60PCT/US97/14070

TABLE 7. cont.

<td></td><td> Exp l</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> Normalized</td><td> Sensor 9</td><td colspan="3"> polyethylene oxide) (not heated)</td><td></td><td></td>

<td> averages</td><td> 22°C</td><td> 22’C</td><td> 2T£</td><td> 221C</td><td> 221C</td><td> 22 °C</td>

<td> propanol</td><td> 7 33E-O2</td><td> 8 62E-02</td><td> 7 74E 02</td><td> 7 45E-O2</td><td> 7 72E 02</td><td> 9 14E-02</td>

<td> benzene</td><td> 1 43E-01</td><td> 1 42ΕΌ1</td><td> 1 43E 01</td><td> 1 44E-O1</td><td> 1 41E01</td><td> 1 38E-01</td>

<td> chloroform</td><td> 1 50E-01</td><td> 1 55EO1</td><td> 1 52EO1</td><td> 1 54E-01</td><td> 1 55EO1</td><td> 1 58E-01</td>

<td> cyclohexane</td><td> 1 78E-02</td><td> 2 23EO2</td><td> 1 78E02</td><td> 1 72E-02</td><td> 1 74E02</td><td> 1 78E02</td>

<td> ethanol</td><td> 6 89E 02</td><td> 7 93E-02</td><td> 6 97E-O2</td><td> 6 73E-O2</td><td> 7 28E-02</td><td> 8 01E-02</td>

<td> hexane</td><td> 1 24E-O2</td><td> 2 OOE-02</td><td> 1 26E-02</td><td> 1 33E-O2</td><td> 1 25E02</td><td> 1 49E-02</td>

<td> methanol</td><td> 3 42E 02</td><td> 4 32E-02</td><td> 3 39E-O2</td><td> 3 43EO2</td><td> 3 78E-02</td><td> 4 32E-02</td>

<td> toluene</td><td> 5 01EO1</td><td> 4 52E-01</td><td> 4 93EO1</td><td> 4 95E-01</td><td> 4 86EO1</td><td> 4 57E-01</td>

<td> Normalized</td><td> Sensor 9</td><td colspan="3"> poly(ethy lene oxtdt.) (not heated)</td><td></td><td></td>

<td colspan="2"> STANDARD DEVIATION</td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> 22*C</td><td> 22X</td><td> 2T£</td><td> 22ZC</td><td></td><td> 22X</td>

<td> 2-propanol</td><td> 3 67E-03</td><td> 3 73E 03</td><td> 1 64E-O3</td><td> 6 77E-04</td><td> 2 83E-03</td><td> 1 00E-02</td>

<td> benzene</td><td> 5 16E-03</td><td> 1 20E-02</td><td> 3 16E-O3</td><td> 2 47EO3</td><td> 9 94E-04</td><td> 2 41E-03</td>

<td> chloroform</td><td> 4 69E-03</td><td> 3 34E-O3</td><td> 1 32E 03</td><td> 1 44E-O3</td><td> 3 40E-03</td><td> 1 08E-02</td>

<td> cyclohexane</td><td> 1 15E-O3</td><td> 4 09E 03</td><td> 7 89E04</td><td> 2 21E-D3</td><td> 2 41E-04</td><td> 1 01E-03</td>

<td> ethanol</td><td> 3 94E 03</td><td> 5 11E-03</td><td> 3 86E-O4</td><td> 1 37EO3</td><td> 4 78E-04</td><td> 4 93E 03</td>

<td> hexane</td><td> 1 90E-03</td><td> 6 97E-03</td><td> 1 12E-O3</td><td> 1 10E-03</td><td> 4 08E-03</td><td> 4 35E-03</td>

<td> methanol</td><td> 1 72E 03</td><td> 2 46E-03</td><td> 6 74EO4</td><td> 1 25E-O3</td><td> 2 04E-03</td><td> 3 49EO3</td>

<td> toluene</td><td> 9 70E 03</td><td> 8 90E-03</td><td> 1 91E-03</td><td> 6 27E-O3</td><td> 1 07E-02</td><td> 8 81E-O3</td>

Printed from Mimosa 03/10/1999 16 43 19 page -62WO 98/07024

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TABLE 7. cont.

<td colspan="3"> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp £</td>

<td></td><td> Normalized</td><td> Sensor 15</td><td colspan="3"> polyethylene oxide)</td><td></td><td></td>

<td> 5</td><td> averages</td><td> 22X</td><td> 22X</td><td> 23 °C</td><td> 44 °C</td><td> 4ZC</td><td> 57°C</td>

<td></td><td> 2-propanol</td><td> 1 I8E-01</td><td> 1 22E-O1</td><td> I 22E-01</td><td> 9 01E-02</td><td> 1 14E-01</td><td> 9 98E-O2</td>

<td></td><td> benzene</td><td> 1 21F01</td><td> 1 27EO1</td><td> 1 20E-01</td><td> 1 14E-01</td><td> 1 43E-01</td><td> 1 06E-01</td>

<td></td><td> chloroform</td><td> 1 52EO1</td><td> 1 52E-O1</td><td> 1 51E-01</td><td> 1 37EO1</td><td> 1 48EO1</td><td> 1 I5EO1</td>

<td> 10</td><td> cyclohexane</td><td> 2 39EO2</td><td> 3 53E-O2</td><td> 2 62EO2</td><td> 1 69E-02</td><td> 4 46E-O2</td><td> 5 62E-O2</td>

<td></td><td> ethanol</td><td> 1 09EO1</td><td> 1 15E-01</td><td> 1 HE 01</td><td> 9 78E02</td><td> 9 05E-02</td><td> 1 14E-01</td>

<td></td><td> hexane</td><td> 1 84E 02</td><td> 2 83E-02</td><td> 1 92E 02</td><td> 3 02E-02</td><td> 3 23E 02</td><td> 2 34E-02</td>

<td></td><td> methanol</td><td> 5 54E02</td><td> 6 39E-02</td><td> 5 71E02</td><td> 5 17E-02</td><td> 4 85E-02</td><td> 7 93E-O2</td>

<td></td><td> toluene</td><td> 4 02E01</td><td> 3 56E-O1</td><td> 3 93EOI</td><td> 4 63E01</td><td> 3 79E-01</td><td> 4 06E-0I</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> Normalized</td><td> Sensor 15</td><td colspan="5"> polyethylene oxide)</td>

<td> 20</td><td colspan="2"> STANDARD DEVIATION</td><td> 2T£</td><td> 23°C</td><td> 44°C</td><td> 4Z£</td><td></td>

<td></td><td> 2-propanol</td><td> 3 12E 03</td><td> 1 56E-O3</td><td> 1 68E-03</td><td> 3 25E-02</td><td> 7 71E-O3</td><td> 3 O5E-O2</td>

<td></td><td> benzene</td><td> 9 84E-04</td><td> 1 82E-O2</td><td> 2 67E-03</td><td> 4 78ΕΌ2</td><td> 1 8 IE-02</td><td> 8O1E-O2</td>

<td></td><td> chloroform</td><td> 4 71E-03</td><td> 7 72E-03</td><td> 1 23E 03</td><td> 2 09E-02</td><td> 1 10E-02</td><td> 3 47E-02</td>

<td></td><td> cyclohexane</td><td> 1 14E-03</td><td> 4 95E-O3</td><td> 5 91E03</td><td> 6 16E-O3</td><td> 2 33E-02</td><td> 7 55E-02</td>

<td> 25</td><td> ethanol</td><td> 3 63E-O3</td><td> 6 63E 03</td><td> 6 06E 03</td><td> 1 02E 02</td><td> 1 08E-02</td><td> 6 71E 02</td>

<td></td><td> hexane</td><td> 3 46E-03</td><td> 3 94E-03</td><td> 5 55E 04</td><td> 3 68E-02</td><td> 1 44E-02</td><td> 1 16E-02</td>

<td></td><td> methanol</td><td> 3 27E-03</td><td> 1 48E-03</td><td> 5 57E-O3</td><td> 9 42E433</td><td> 1 56E 02</td><td> 4 16E-02</td>

<td></td><td> toluene</td><td> 5 22E 03</td><td> 1 49E-02</td><td> 4 32E-O3</td><td> 9 75E 03</td><td> 1 71E-02</td><td> 7 70E-02</td>

Printed from Mimosa 03/10/1999 16 43 19 page -63WO 98/07024

-62PCT/US97/14070

TABLE 7 .cont.

<td></td><td> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td> Normalized</td><td> Sensor 16</td><td colspan="3"> polyethylene oxide)</td><td></td><td></td>

<td> averages</td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> 2TC</td><td> 222C</td><td> ax</td><td> 33°C</td><td> 33 °C</td><td> 27X</td>

<td> 2-propanol</td><td> 6 88E 02</td><td> 7 87E-O2</td><td> 7 22E 02</td><td> 6 11E-02</td><td> 6 60E-02</td><td> 6 75E-02</td>

<td> benzene</td><td> 1 4SE-01</td><td> 1 42E-01</td><td> 1 46E-0I</td><td> 1 51E-01</td><td> 1 50E-01</td><td> 1 47E-01</td>

<td> chloroform</td><td> 1 48E-01</td><td> 1 S2E 01</td><td> 1 51E-01</td><td> 1 39E-O1</td><td> 1 41Ε-Ό1</td><td> 1 38E-O1</td>

<td> cyclohexane</td><td> 1 53E-O2</td><td> 1 73E-O2</td><td> 1 55E-O2</td><td> I 72E-02</td><td> 1 78E-02</td><td> 2 06E-02</td>

<td> ethanol</td><td> 6 26E-O2</td><td> 7 29E-02</td><td> 6 54E-O2</td><td> 5 69E-O2</td><td> 6 03E-02</td><td> 6 38E-02</td>

<td> hexane</td><td> 9 63E-O3</td><td> 1 33E-02</td><td> 1 07E-02</td><td> 1 16E-O2</td><td> 1 28E-02</td><td> 1 50E-02</td>

<td> methanol</td><td> 3 00E02</td><td> 3 68E-O2</td><td> 3 HE 02</td><td> 2 91E-02</td><td> 3 09E-02</td><td> 3 60E-02</td>

<td> toluene</td><td> 5 21E-01</td><td> 4 86E-O1</td><td> S 08E 01</td><td> 5 33E-O1</td><td> 5 21E-01</td><td> 5 12E-01</td>

<td> Normalized</td><td> Sensor 16</td><td colspan="3"> polyethylene oxide)</td><td></td><td></td>

STANDARD DEVIATION

<td></td><td> 22°C</td><td> 22X</td><td> 23°C</td><td> 22X</td><td> arc</td><td> 37 °C</td>

<td> 2-propanol</td><td> 2 68E-O3</td><td> 2 04E-03</td><td> 6 52E-04</td><td> 1 39E-03</td><td> 8 06E-04</td><td> 2 45E 03</td>

<td> benzene</td><td> 4 83E-03</td><td> 4 29E 03</td><td> 1 12E-03</td><td> 1 31E-O3</td><td> 2 29E-03</td><td> 5 57E-O3</td>

<td> chloroform</td><td> 7 22E 03</td><td> 2 08E-03</td><td> 2 94E-03</td><td> 1 55E-O3</td><td> 2 29E 03</td><td> 6 97ΕΌ3</td>

<td> cyclohexane</td><td> 1 60E-04</td><td> 1 86E-O3</td><td> 2 13E-O4</td><td> 1 39E 03</td><td> 1 51E 03</td><td> 8 98E-O4</td>

<td> ethanol</td><td> 2 89E-03</td><td> 2 78E-03</td><td> 3 73E04</td><td> 9 62E-04</td><td> 4 09E 04</td><td> 2 02E-03</td>

<td> hexane</td><td> 4 86E-04</td><td> 2 03E-03</td><td> I 21E-04</td><td> 9 89E-04</td><td> 8 28E-04</td><td> 1 62E 03</td>

<td> methanol</td><td> 4 58E-04</td><td> 1 31EO3</td><td> 7 76E-04</td><td> 3 98E-O4</td><td> 1 58E-O3</td><td> 8 50E-04</td>

<td> toluene</td><td> 9 39E-03</td><td> 6 32E-O3</td><td> 4 35E 03</td><td> 7 19E-O3</td><td> 6 42ΕΌ3</td><td> 5 87E-O3</td>

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TABLE 7. cont.

<td colspan="2"></td><td> Expl</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td></td><td> Normalized</td><td> Sensor 5</td><td colspan="3"> poly(4-vinyl phenol) (not heated)</td><td></td><td></td>

<td> 5</td><td> averages</td><td> 22°C</td><td> 2T£</td><td> 23°C</td><td> 22°C</td><td> 22°C</td><td> 22°C</td>

<td></td><td> 2-propanol</td><td> 9 08E-02</td><td> 5 67E-02</td><td> 7 83E 02</td><td> 9 08E-O2</td><td> 7 68E-O2</td><td> 5 63E-O2</td>

<td></td><td> benzene</td><td> 1 40E-02</td><td> 122E-02</td><td> 1 46E-02</td><td> 1 49E-02</td><td> 1 41E-02</td><td> 1 4SE-02</td>

<td></td><td> chloroform</td><td> 2 73E-02</td><td> 2 26EO2</td><td> 2 76E-02</td><td> 3 00E-02</td><td> 2 82E-O2</td><td> 2 19E-02</td>

<td> 10</td><td> cyclohexane</td><td> 3 62E-03</td><td> 2 77E-03</td><td> 3 05E-03</td><td> 2 I7E-03</td><td> 2 79E-03</td><td> 3 72E-03</td>

<td></td><td> ethanol</td><td> 4 81E-01</td><td> 4 71E-01</td><td> 4 81E-01</td><td> 4 75EO1</td><td> 4 77E-01</td><td> 4 68E-01</td>

<td></td><td> hexane</td><td> 3 65E-O3</td><td> 3 72E-03</td><td> 3 67E-03</td><td> 4 97E-03</td><td> 3 45EO3</td><td> 3 92E-03</td>

<td></td><td> methanol</td><td> 3 50E 01</td><td> 4 06E-01</td><td> 3 62E-01</td><td> 3 51E-01</td><td> 3 71E-01</td><td> 4 08E-01</td>

<td></td><td> toluene</td><td> 2 98E-02</td><td> 2 49E-02</td><td> 2 98E-O2</td><td> 3 09E-02</td><td> 2 73E-02</td><td> 2 46E-02</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> Normalized</td><td> Sensor 5</td><td colspan="3"> poly(4-vinyl phenol) (not heated)</td><td></td><td></td>

STANDARD DEVIATION

<td> 20</td><td></td><td> 22°C</td><td> 22X</td><td> 23°C</td><td> 22C</td><td> 22°C</td><td> 2TC</td>

<td></td><td> 2-propanol</td><td> 3 47E-03</td><td> 2 54E-03</td><td> I 62EO3</td><td> 2 13E-03</td><td> 2 63EO3</td><td> 3 99E-03</td>

<td></td><td> benzene</td><td> 5 06E-04</td><td> 1 03E-03</td><td> I 30E 03</td><td> 1 14E-03</td><td> 6 17E-04</td><td> 8 88E-04</td>

<td></td><td> chloroform</td><td> 1 85E 03</td><td> 1 25E-03</td><td> 6 47E-O4</td><td> 1 66E-03</td><td> 7 39E-O4</td><td> 1 63E-03</td>

<td></td><td> C clohexane</td><td> 4 16E-04</td><td> 1 17EO3</td><td> 4 08EO4</td><td> 1 46E-03</td><td> 5 2IE-04</td><td> 2.23E-03</td>

<td> 25</td><td> ethanol</td><td> 1 55E-O2</td><td> 1 84E-02</td><td> 4 28E-03</td><td> 1 48E-02</td><td> 1 05E-02</td><td> 2 24E-02</td>

<td></td><td> hexane</td><td> 9 72EO4</td><td> 3 32E-04</td><td> 9 08E-04</td><td> 9 08E-04</td><td> 1 21EO4</td><td> 9 83E-04</td>

<td></td><td> methanol</td><td> 1 46E 03</td><td> 6 28EO3</td><td> 4 61E-O3</td><td> 4 63E-O3</td><td> 3 12E-03</td><td> 1 44E-02</td>

<td></td><td> toluene</td><td> 1 39E-03</td><td> 1 77E-03</td><td> 2 80E-03</td><td> 2 63E-O3</td><td> 1 51E-03</td><td> 2 14E-04</td>

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TABLE 7, cont.

<td colspan="3"> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td></td><td> Normalized</td><td> Sensor 7</td><td colspan="3"> poly(4 vinyl phenol) (not heated)</td><td></td><td></td>

<td> 5</td><td> averages</td><td> 22° C</td><td> 22°C</td><td> 221C</td><td> 22*C</td><td> 22°C</td><td> 22°C</td>

<td></td><td> 2-propanol</td><td> S69EO2</td><td> 1 49E-02</td><td> 4 41E-02</td><td> S 4SE-O2</td><td> 3 01E-O2</td><td> 1 6OE-O2</td>

<td></td><td> benzene</td><td> 2 55E-O2</td><td> 1 69E-02</td><td> 1 86E-02</td><td> 1.66E-02</td><td> 1 61E-02</td><td> 1 88E-O2</td>

<td></td><td> chloroform</td><td> 2 15E-02</td><td> 1 31EO2</td><td> 1 95E-02</td><td> 2 46E-02</td><td> 1 61EO2</td><td> 1 29E-O2</td>

<td> 10</td><td> cyclohexane</td><td> 1 23E-02</td><td> 4 79E-03</td><td> 4 71E-03</td><td> 6 02E-03</td><td> 3 69E-03</td><td> 8 74E-O3</td>

<td></td><td> ethanol</td><td> 4 39E-O1</td><td> 2 91E-01</td><td> 4 27E-01</td><td> 4 35E-01</td><td> 3 64E-01</td><td> 2 67E-01</td>

<td></td><td> hexane</td><td> 6 74E-C3</td><td> 9 58EO3</td><td> 8 68E-03</td><td> 6 40E-03</td><td> 6 52E-03</td><td> 8 15E-O3</td>

<td></td><td> methanol</td><td> 3 99E-O1</td><td> 611E-01</td><td> 4 42E-01</td><td> 4 22E-01</td><td> 5 33E-01</td><td> 6 33E-O1</td>

<td></td><td> toluene</td><td> 3 87E-02</td><td> 3 84E-02</td><td> 3 62E-02</td><td> 3 48E-02</td><td> 3 04E-02</td><td> 3 50E-02</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> Normalized</td><td> Sensor 7</td><td colspan="3"> poly(4-vinyl phenol) (not heated)</td><td></td><td></td>

<td></td><td colspan="2"> STANDARD DEVIATION</td><td></td><td></td><td></td><td></td><td></td>

<td> 20</td><td></td><td> 2¾ °C</td><td> 22 °C</td><td> 2ΤΩ</td><td> 22^</td><td> 22° C</td><td> 22°C</td>

<td></td><td> 2-propanol</td><td> 4 99E-O3</td><td> 2 48E 03</td><td> 3 31E-03</td><td> S 81E 03</td><td> 2 27E-03</td><td> 3 33E-03</td>

<td></td><td> benzene</td><td> 4 26E-04</td><td> 4 91EO3</td><td> 3 43EO3</td><td> 3 84E-O3</td><td> 3 64E-03</td><td> 2 57E-03</td>

<td></td><td> chloroform</td><td> 2 35EO3</td><td> 7 22E-03</td><td> 3 06E-03</td><td> 4 90E-03</td><td> 5 65E-O3</td><td> 2 22E-03</td>

<td></td><td> cyclohexane</td><td> 1 52EO3</td><td> 2 16E-03</td><td> 2 30E 03</td><td> 2 45E-03</td><td> 1 53E 03</td><td> 5 O3E-O3</td>

<td> 25</td><td> ethanol</td><td> 3 08E 02</td><td> 2 83E-O2</td><td> 9 78E-O3</td><td> 1 60E-02</td><td> 2 21E-02</td><td> 1 47E-02</td>

<td></td><td> hexane</td><td> 4 12E-O3</td><td> 3 55E-03</td><td> 3 95E 03</td><td> 9 80E-04</td><td> 1 80E-03</td><td> 4 26E-03</td>

<td></td><td> methanol</td><td> 7 23E-O3</td><td> 3 3OE-O2</td><td> 9 99E-O3</td><td> 1 47E-02</td><td> 2 12E-02</td><td> 1 71E 02</td>

<td></td><td> toluene</td><td> 2 99E-O3</td><td> 5 36E-O3</td><td> 3 72E-O3</td><td> 1 39E-O3</td><td> 3 49EO3</td><td> 4 87E-03</td>

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TABLE 7. cont.

<td colspan="3"> &amp;q»l</td><td> Exp 3</td><td> ExpS</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td></td><td> Normalised</td><td> Sensor 18</td><td colspan="3"> poly(4-vmyl phe-nol)</td><td></td><td></td>

<td> 5</td><td> averages</td><td> 22X</td><td> 22‘C</td><td> 23°C</td><td> 45°C</td><td> 46°C</td><td></td>

<td></td><td> 2-propanol</td><td> 1 53E-O2</td><td> 3 25E-02</td><td> 1 70E-02</td><td> 4 70E-02</td><td> 2 80E-02</td><td> 1ME-01</td>

<td></td><td> benzene</td><td> 7 26E-03</td><td> 6 05E-02</td><td> 5 08E-02</td><td> 2 14E-O2</td><td> 6 61E-02</td><td> 5 69E-02</td>

<td></td><td> cnloroform</td><td> 6 33E-02</td><td> 1 43E-01</td><td> 4 60E-02</td><td> 7 52E-O2</td><td> 3 42E-02</td><td> 1 06E-01</td>

<td> 10</td><td> cyclohexane</td><td> 5 52E-02</td><td> 1 81E-01</td><td> 2 34E-O2</td><td> 3 20E-O2</td><td> 4 77E-02</td><td> 7 52E-02</td>

<td></td><td> ethanol</td><td> 9 79E-02</td><td> 9 42E-02</td><td> 9 45E-O2</td><td> 2 89E-01</td><td> 3 57E-02</td><td> 2 24EO1</td>

<td></td><td> hexane</td><td> 3 66E-O2</td><td> 7 66E02</td><td> 2.34EO2</td><td> 6556EO2</td><td> 4 18E-O2</td><td> 1 10E-01</td>

<td></td><td> methanol</td><td> 6 4SE-01</td><td> 3 68E-O1</td><td> 7 24E-01</td><td> 4 88E-O1</td><td> 6 75E 01</td><td> 2 60E-01</td>

<td></td><td> toluene</td><td> 7 89E-02</td><td> 4 50E-02</td><td> 2 14E-02</td><td> -1 77E-02</td><td> 7 10E-02</td><td> 6 11E-02</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> Normalized STANDARD I</td><td> Sensor 18 )EV1ATION</td><td colspan="2"> poly(4-vtnyl phenol)</td><td colspan="3"> ' 1</td>

<td> 20</td><td></td><td> 22°C</td><td> 22°C</td><td> 2T£</td><td> 45°C</td><td></td><td> 6TC</td>

<td></td><td> 2-propanol</td><td> 8 51E-03</td><td> 5 20E-02</td><td> 8 77E-03</td><td> 1 90E-02</td><td> 4 51E-02</td><td> 6 73E-02</td>

<td></td><td> benzene</td><td> 6 45E-O2</td><td> 7 58E-O2</td><td> 3 51E-02</td><td> 4 86E-O2</td><td> 3 51E-O3</td><td> 4 31E-02</td>

<td></td><td> chloroform</td><td> 4 47E-02</td><td> 8 22E-03</td><td> 2 99E-02</td><td> 6 17E-O2</td><td> 2 13EO2</td><td> 1 59E-02</td>

<td></td><td> cj clohexane</td><td> 4 68E 02</td><td> 2 43EOI</td><td> 2 87E-02</td><td> 9 01E-02</td><td> 1 71E-O2</td><td> 3 73E-02</td>

<td> 25</td><td> ethanol</td><td> 3 26E-02</td><td> 4 93E-02</td><td> 1 79E-02</td><td> 4 47E-O2</td><td> 4 74E-O2</td><td> 2 08E-01</td>

<td></td><td> hexane</td><td> 2 05E-02</td><td> 5 31E-O2</td><td> 1 16E-02</td><td> 3 26E-02</td><td> 1 67E 02</td><td> 4 25E02</td>

<td></td><td> methanol</td><td> 1 77EO1</td><td> 1 09E-01</td><td> 2 25E-02</td><td> 2 14E-01</td><td> 9 03E-02</td><td> 1 30E 01</td>

<td></td><td> toluene</td><td> 7 92E 02</td><td> 1 11E-01</td><td> 1 72E-02</td><td> 7 21E-02</td><td> 2 90E-02</td><td> 6.50E-03</td>

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TABLE 7. cnnt.

<td colspan="3"> Exp 1</td><td> Exp 3</td><td> Exp 5</td><td> Exp 2</td><td> Exp 4</td><td> Exp 6</td>

<td></td><td> Norma'ized</td><td> Sensor 19</td><td colspan="3"> poly(4-vinyl phenol)</td><td></td><td></td>

<td> 5</td><td> averages</td><td> 22° C</td><td></td><td> 23°C</td><td> 37°C</td><td></td><td></td>

<td></td><td> 2 propanol</td><td> 3 84E-O2</td><td> 1 35EO2</td><td> 1 16E-02</td><td> 6 17E-O2</td><td> 2 37E-02</td><td> 2 38E-O2</td>

<td></td><td> benzene</td><td> 9 77EO3</td><td> 1 21EO2</td><td> 1 39E-02</td><td> 1 57E-O2</td><td> 1 31E-02</td><td> 1 77EO2</td>

<td></td><td> chloroform</td><td> 1 65E-02</td><td> 7 41EO3</td><td> 1 06E02</td><td> 2 OtE-02</td><td> 1 52E-02</td><td> 1 19E-O2</td>

<td> 10</td><td> cyclohexane</td><td> 5 52E-03</td><td> 4 73EO3</td><td> 7 2SE-03</td><td> 2 86ΕΌ3</td><td> 6 85E-03</td><td> 1 72E-02</td>

<td></td><td> ethanol</td><td> 4 62E-01</td><td> 3 68E-OI</td><td> 3 43E-01</td><td> 4 67EO1</td><td> 4 15ΕΌ1</td><td> 3 28E-01</td>

<td></td><td> hexane</td><td> 3 50E-03</td><td> 5 55E-O3</td><td> 6 42E-03</td><td> 2 72E-O3</td><td> 6 25E-03</td><td> 2 29E-02</td>

<td></td><td> methanol</td><td> 4 46E-01</td><td> 5 66E-01</td><td> 5 78E01</td><td> 4 09EO1</td><td> 5 02E-01</td><td> 5 56E-01</td>

<td></td><td> toluene</td><td> 1 93E-02</td><td> 2 33E-O2</td><td> 2 89E-O2</td><td> 2 14E-02</td><td> 1 87E 02</td><td> 2 23EO2</td>

<td> 15</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td> Normalized</td><td> Sensor 19</td><td colspan="5"> poly(4-\inyl phenol)</td>

<td> 20</td><td colspan="2"> STANDARD DEVIATION 22°C</td><td> 22X</td><td> 2T£</td><td> 37°C</td><td> 38°C</td><td> 43°C</td>

<td></td><td> 2 propanol</td><td> 6 85E-04</td><td> 2 48E-03</td><td> 3 81E-O3</td><td> 3 46E-O3</td><td> 1 O8E-O2</td><td> 1 19E-02</td>

<td></td><td> benzene</td><td> 6 75E-04</td><td> 4 50E-03</td><td> 4 29E 03</td><td> 8 71E-O3</td><td> 3 80EO3</td><td> 3 52E-03</td>

<td></td><td> chloroform</td><td> 2 88E 03</td><td> 1 62E-O3</td><td> 1 I4E-03</td><td> 1 79E-03</td><td> 7 79EO3</td><td> 5 08E-03</td>

<td></td><td> cyclohexane</td><td> 5 59E-04</td><td> 2 80E-03</td><td> 4 95E 03</td><td> 3 84E-O3</td><td> 2 85EO3</td><td> 4 25Ε-Ό3</td>

<td> 25</td><td> ethanol</td><td> 2 33E-02</td><td> 3 40E-02</td><td> 1 98E-03</td><td> 4 30E-02</td><td> 4 69E 02</td><td> 5 96E-02</td>

<td></td><td> hexane</td><td> 2 04E-03</td><td> 3 93E-04</td><td> 2 07E 03</td><td> 6O2E-O3</td><td> 8 91E-04</td><td> 9 96E 03</td>

<td></td><td> methanol</td><td> 9 31E-03</td><td> 2 57E-02</td><td> 6 54E-O3</td><td> 3 61E-O2</td><td> 1 90E-02</td><td> 5 43E-02</td>

<td></td><td> toluene</td><td> 3 81E-03</td><td> 1 28E-03</td><td> 6 39EO3</td><td> 1 O2E-O3</td><td> 1 19E-02</td><td> 2 38E 02</td>

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The average AR/R values for exposures to the eight solvents are listed in Table 6 and are presented graphically in the various panels of Figure 11 It can be seen that as the temperature increases, the over-all response of a given sensor decreases (in accord with the expected decrease in gas/polymer partition coefficient with increasing temperature) In addition, the error bars became larger at the higher temperatures as the signal became smaller relative to the noise

Because of the differences in over-all height of the responses, the differoiceis in response patterns between the room temperature data and the elevated temperature data are difficult to visualize from the plots of the absolute AR/R response of the sensor array The patterns were, therefore, normalized by adding all the responses by a sensor to the eight solvents and dividing this sum by each response to a solvent This normalization process highlights the differences in the pattern of responses at different temperatures rather than the over-all height d^ee^ces Table 7 and the various panels of Figure 12 show the normalized patterns for the sensors

In general, the fingerprints for the various vapors were similar at the different temperatures, but the magnitudes of the absolute responses were quite different This implies that the characteristic ''fingerprint” pattern of the analytes chosen in this study, for the sensor films investigated, were essc^i^tt^lly invariant over the sensor matenals and temperature range probed herein This will be beneficial in many applications, (quality control of foodstuffs, process monitoring, etc ) in that identification of a vapor or (of its constancy m composition) over various exposures to the array, and classification of the vapor type in any given exposure, using the response pattern of the sensor output signals will not significantly depend on the temperature of the sensor array or of the analyte

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Quantitive analysts of the concentration of the analyte, would, however, require knowledge of the sensor temperature This effect can be used to advantage in other potential applications of these sensor arrays, since lowering the temperature of an array element will m general increase the signal and therefore increase the sensitivity of the sensor to the desired analyte Thus, a plurality of compositionally identical sensors, each held at a different temperature during a measurement period, could be used to produce signals above a threshold value at different concentrations of the vapor, thereby aiding m quantifying various ranges of the vapor concentration while still maintaining a linear concentration vs vapor concentration response (in the small swelling regime) for an individual sensor In addition, the differential response of compositionally identical sensors at various temperatures can be used to provide classification and identification information as the basis for the output signature of the sensor array In general, combinations of compositionally different sensors at a plurality of different temperatures will produce a more desirable, more informat'on-nch, data set from a given set of sensor materials than measurements at one fixed temperature, and can such arrays can therefore be usefully exploited for the purpose of detection, identification, and quantification, of a particular analyte Such detection, identification and quantitiation may also be performed by employing a single resistor at a plurality of different temperatures

All publications and patent applications cited in this specification are herein incorporated by reference as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and

Printed from Mimosa 03/10/1999 16 43 19 page -70WO 98/07024

-69PCT/US97/14070 modifications may be made thereto without departing from the spirit or scope of the appended claims