Process to obtain insulin with correct cystin bridges

(57) A summary and the purpose Offer of the method of obtaining the insulin which has the cystine bridge construction connected correctly. Composition While carrying out direct conversion of the pro insulin to an insulin using enzyme and separating an insulin from the reaction mixed-solution directly using hydrophobic adsorption resin, In the bottom of existence of mercaptan and a カオトロ pick auxiliary agent, and pH 10*11, the insulin which has the cystine bridge construction connected correctly is obtained from a corresponding pro insulin amino acid chain.