High arachidonic acid producing strains of yarrowia lipolytica
Abstract
Engineered strains of the oily yeast Yarrowia lipolytica are capable of producing more than 25% eicosaspentaenoic acid (EPA), a (? -3 polyunsaturated fatty acid) in the total oil fraction described. These strains include various chimeric genes expressing heterologous desaturases, elongases, and acyltransferases, and optionally include various native desaturase and acyltransferase knockouts to enable synthesis and high EPA accumulation. Production host cells are required, as are methods for producing EPA with said host cell.
Term
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1 claim: 1 independent, 0 dependent
- 1Δ17 (DGLA) denturaM ik Echosacetic acid p »A®-ai (ETA) Δ17 (DGLA) denturaM ψ Eikosatetmnsyre p»A®-ai (ETA) Eicostric acid (C20:3, e-3J (ΕΓΛ} Eikostriensyre (C20:3,e-3J (ΕΓΛ} Amkidonic Acid [C20: 4, e> -6] (ARA) Amkidonsyre [C20:4, e>-6] (ARA) A17 [ · deestoraee ψ A17 [· deestoraee ψ ABdawhy Eicosapentaenoic Acid [C2 & 5, b-31 (EPA) ABdawhy Eikosapentaensyre [C2&5, b-31 (EPA)
10 paragraphs in 2 sections, as filed
A23K 1/16 (2006.01)
A23L 1/30 (2006.01)
Patentstyret
<td> (21)</td><td> Søknadsnr</td><td> 20072575</td><td> (86)</td><td> Innt.inng.dag og søknadsnr</td><td> 2005.11.03 PCT/US05/040255</td>
<td> (22)</td><td> Inng.dag</td><td> 2007.05.21</td><td> (85)</td><td> Videreføringsdag</td><td> 2007.05.21</td>
<td> (24)</td><td> Løpedag</td><td> 2005.11.03</td><td> (30)</td><td> Prioritet</td><td> 2004.11.04, US, 60/624,812</td>
<td> (41)</td><td> Alm.tilgj</td><td> 2007.08.01</td><td></td><td></td><td></td>
(71) Søker (72) Oppfinner (74) Fullmektig
EI du Pont de Nemours and Co, 1007 Market Street, DE19898 WILMINGTON, US Stephen K Picataggio, 17 Meadow Wood Lane, PA19350 LANDENBERG, US Hongxiang Zhang, 3808 Rotherfield Lane, PA19317 CHADDS FORD, US Quinn Qun Zhu, 544 Revere Road, PA19382 WEST CHESTER, US Howard G Damude, 4 Kenwick Road, DE19707 HOCKESSIN, US Peter John Gillies, 501 Port Royal Court, PA19350 LANDENBERG, US Daniel Joseph Macool, 2525 Meredith Street, PA19130 PHILADELPHIA, US James John Ragghianti, 15 Decidedly Lane, DE19701 BEAR, US Zhixiong Xue, 111 Harvey Lane, PA19317-9728 CHADDS FORD, US Dana M Walters Poliak, 123c East Baker Street, PA19063 MEDIA, US Narendra S Yadav, 3704 Knole Lane, PA19317 CHADDS FORD, US Zacco Norway AS, Postboks 2003 Vika, 0125 OSLO, NO (54) Benevnelse Stammer av Yarrowia Lipolytica som har høy produksjon av eikosapentaensyre (57) Sammendrag
Konstruerte stammer av den oljeholdige gjæren Yarrowia lipolytica er i stand til å produsere mer enn 25% eicosaspentaensyre (EPA), en (ω-3 polyumettet fettsyre) i den totale oljefraksjonen som er beskrevet. Disse stammene omfatter ulike kimære gener som uttrykker heterologe desaturaser, elongaser og acyltransferaser og omfatter eventuelt ulike native desaturase og acyltransferase knockouts for å muliggjøre syntese og høy akkumulering av EPA. Produksjonsvertsceller er stilt krav om, som også fremgangsmåter for å produsere EPA med nevnte vertseeller.
SBaiinsyre \ Palmitate _ Myristinsyre [Cl 8:0] Citna dengen |CW:0] Cweeiongna [C14:0]
<img file="NO20072575A_D0001.tif" />
Contents2
1 sheet
Sheet 1
124 members in 11 offices
Priority claims8
| Document | Office | Kind | Date |
|---|---|---|---|
| 62481204 | United States of America | P | |
| 62481204 | United States of America | P | |
| 2005040255 | United States of America | W | |
| 2005040255 | United States of America | W | |
| 60624812 | – | – | – |
| PCTUS05040255 | – | – | – |
| US20040624812P | – | – | – |
| WO2005US40255 | – | – | – |
Members124
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| AU2005267202A1 | Australia | A1 | |
| CA2568624A1 | Canada | A1 | |
| WO2006012325A1 | World Intellectual Property Organization (WIPO) | A1 | |
| WO2006012326A1 | World Intellectual Property Organization (WIPO) | A1 | |
| US2006094086A1 | United States of America | A1 | |
| US2006094087A1 | United States of America | A1 | |
| US2006094088A1 | United States of America | A1 | |
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| US2006094102A1 | United States of America | A1 | |
| CA2584719A1 | Canada | A1 | |
| CA2585235A1 | Canada | A1 | |
| CA2585404A1 | Canada | A1 | |
| WO2006052754A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2006052807A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2006052814A2 | World Intellectual Property Organization (WIPO) | A2 | |
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| WO2006052870A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2006052871A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2006052912A2 | World Intellectual Property Organization (WIPO) | A2 | |
| WO2006052914A2 | World Intellectual Property Organization (WIPO) | A2 | |
| US2006110806A1 | United States of America | A1 | |
| CA2585178A1 | Canada | A1 | |
| WO2006055322A2 | World Intellectual Property Organization (WIPO) | A2 | |
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| US2006195939A1 | United States of America | A1 | |
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| EP1758991A1 | European Patent Office (EPO) | A1 | |
| US7189559B2 | United States of America | B2 | |
| US7192762B2 | United States of America | B2 | |
| US7198937B2 | United States of America | B2 | |
| EP1807525A2 | European Patent Office (EPO) | A2 | |
| EP1807526A2 | European Patent Office (EPO) | A2 | |
| EP1807527A2 | European Patent Office (EPO) | A2 | |
| NO20072517L | Norway | L | |
| EP1809756A2 | European Patent Office (EPO) | A2 | |
| NO20072575LThis record | Norway | L | |
| NO20072516L | Norway | L | |
| US2007184538A1 | United States of America | A1 | |
| WO2006052870A3 | World Intellectual Property Organization (WIPO) | A3 | |
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| KR20070085665A | Republic of Korea | A | |
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| CN101111601A | China | A | |
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| WO2006055322A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2006052914A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2006052807A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2006052871A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2006052912A3 | World Intellectual Property Organization (WIPO) | A3 | |
| WO2006052814A3 | World Intellectual Property Organization (WIPO) | A3 | |
| US2009104674A1 | United States of America | A1 | |
| US7524658B2 | United States of America | B2 | |
| EP1807526A4 | European Patent Office (EPO) | A4 | |
| WO2006052824A3 | World Intellectual Property Organization (WIPO) | A3 | |
| CN101437951A | China | A | |
| CN101437952A | China | A | |
| CN101437953A | China | A | |
| US7550286B2 | United States of America | B2 | |
| US7550651B2 | United States of America | B2 | |
| EP1807527A4 | European Patent Office (EPO) | A4 | |
| US7588931B2 | United States of America | B2 | |
| US2009264666A1 | United States of America | A1 | |
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| US2009325265A1 | United States of America | A1 | |
| US2010022647A1 | United States of America | A1 | |
| EP1807525A4 | European Patent Office (EPO) | A4 | |
| US2010068789A1 | United States of America | A1 | |
| US2010105614A1 | United States of America | A1 | |
| EP1809756A4 | European Patent Office (EPO) | A4 | |
| US7879591B2 | United States of America | B2 | |
| US2011077200A1 | United States of America | A1 | |
| US2011086919A1 | United States of America | A1 | |
| US7932077B2 | United States of America | B2 | |
| AU2005267202B2 | Australia | B2 | |
| EP2458000A1 | European Patent Office (EPO) | A1 | |
| CN101437953B | China | B | |
| JP5080979B2 | Japan | B2 | |
| US8323960B2 | United States of America | B2 | |
| US2013005655A1 | United States of America | A1 | |
| EP1758991B1 | European Patent Office (EPO) | B1 | |
| JP5129574B2 | Japan | B2 | |
| JP5139806B2 | Japan | B2 | |
| US2013123361A1 | United States of America | A1 | |
| EP1807527B1 | European Patent Office (EPO) | B1 | |
| EP2458000B1 | European Patent Office (EPO) | B1 | |
| US8518674B2 | United States of America | B2 |
2 legal events, as the office reported them to INPADOC
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| Withdrawal, rejection or dismissal of laid open patent applicationFC2A | FC2A | |
| Withdrawal, rejection or dismissal of laid open patent applicationFC2A | FC2A |
Numbers
- Publication, DOCDB
- 20072575
- Publication, EPODOC
- NO20072575L
- Application
- 2575
- Application, DOCDB
- 20072575
- Application, EPODOC
- NO20070002575
Titles2
- Norwegian
- Stammer av Yarrowia Lipolytica som har hoy produksjon av eikosapentaensyre
- English
- Strains of Yarrowia Lipolytica which have high production of eicosapentaenoic acid
Classification
- CPC, 36
- A23D9/00
- C12P7/6434
- C12P7/64
- A23V2002/00
- C12N1/16
- C12N9/0083
- C12N9/1029
- C12N15/52
- C12N15/746
- C12P7/6472
- A23K20/158
- A23L33/12
- A61P1/00
- A61P1/04
- A61P1/14
- A61P11/00
- A61P17/02
- A61P19/02
- A61P19/10
- A61P25/00
- A61P25/20
- A61P25/24
- A61P25/28
- A61P25/32
- A61P27/02
- A61P29/00
- A61P3/00
- A61P3/06
- A61P43/00
- A61P9/00
- A61P9/10
- A61P9/12
- A61P3/10
- C12N15/74
- C12N1/18
- C12N15/815
- IPC, 7
- C12P7 6434
- A23K1 16
- A23L1 30
- A23L27 00
- C07H21 04
- C12N1 20
- C12P7 6472