Nutritional products having improved organoleptic properties.

(54) Title: NUTRITIONAL PRODUCTS THAT HAVE IMPROVED ORGANOLEPTIC PROPERTIES.

(54) Title: NUTRITIONAL PRODUCTS HAVING IMPROVED ORGANOLEPTIC PROPERTIES.

(57) Summary

The present invention relates to nutritional products comprising pea protein hydrolysates as the primary or sole protein source. Nutritional products include liquids, powders, nutritional bars, and other nutritional products, where the resulting nutritional products are physically stable during shelf life and provide favorable organoleptic properties. These nutritional products can be used as a food source for individuals who are intolerant and / or allergic to cow's milk proteins and soy and oo proteins who suffer from malabsorption, maldigestion, or other gastrointestinal conditions.

(57) Abstract

Disclosed are nutritional products comprising pea protein hydrolysates as a primary or sole source of protein. The nutritional products inelude nutritional liquids, powders, bars, and other food products, wherein the resultaling nutritional products are physically slable over shelf life and provide favorable organoleptic properties. These nutritional products may be used as a nulrilional source for individuals who are intolerant and / or allergic to cow milk proteins and soy proteins and / or who have malabsorption, maldigestion, or other gasíroinsíinal condiíions.

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PATENT TITLE No. 347477

Owner (s): ABBOTT LABORATORIES

Home: ¹⁰⁰ Abbott Park Road Dept. 0377 AP6A-1, Abbott Park, Illinois, 60064, USA

Denomination: NUTRITIONAL PRODUCTS THAT HAVE PROPERTIES

IMPROVED ORGANOLEPTICS.

Classification: CIP: A23L29 / 206; A61K36 / 48; A61K38 / 01

CPC: A23L29 / 206; A23L2 / 66; A23L33 / 40; A61K36 / 48; A61K38 / 011

Inventories): CHRISTOPHER T. CORDLE; STEVEN T. LUEBBERS; LARRY W. WILLIAMS;

JEFFREY HARRIS BAXTER; GERALYN DUSKA-MCEWEN

REQUEST

Number: International filing date:

MX / a / 2013/002154 August 22, 2011

PRIORITY

Country: ....... Date: ........... Number:

US August 24, 2010 61 / 376,503

Validity: Twenty years .....

Expiration Date: August 22, 2031

Issue Date: April 27, 2017

The reference patent is granted based on articles 1, 2, section V, 6, section III, and 59 of the Industrial Property Law.

In accordance with article 23 of the Industrial Property Law, this patent is valid for twenty years, non-extendable, counted from the filing date of the application and will be subject to the payment of the fee to keep the rights in force.

Whoever signs this title does so based on the provisions of articles 6, sections III and 7^or bis 2 of the Industrial Property Law (Official Gazette of the Federation (DOF) 06/27/1991. amended on 08/02/1994 10/25/1996, 12/26/1997, 05/17/1999, 26 / 01/2004, 06/16/2005, 01/25/2006, 05/06/2009, 01/06/2010, 06/18/2010, 06/18/2010, 01/27/2012 and 04/09 2012), articles 11, 3^or fraction V part a), 4^or and 12th sections I and III of the Regulations of the Mexican Institute of Industrial Property (DOF 12/14/1999, amended on 07/01/2002, 07/15/2004, 07/28/2004 and 09/07/2007) ; Articles 1, 3, 4^or, 5^or Section V subsection a), 16 sections I and III and 30 of the Organic Statute of the Mexican Institute of Industrial Property (DOF 12/27/1999, amended on 10/10/2002, 07/29/2004, 08/04/2004 and 09/13/2007). 1st. 3rd and 5th a) of the Agreement that delegates powers to the Deputy General Directors, Coordinator, Divisional Directors, Heads of Regional Offices, Divisional Deputy Directors, Departmental Coordinators and other subordinates of the Mexican Institute of Industrial Property. (DO F, 12/15/1999, amended on 02/04/2000, 07/29/2004, 08/04/2004 and 09/13/2007).

This document is signed with an advanced electronic signature (FIEL), based on articles 7 BIS 2 of the Industrial Property Law; 3 of its Regulations, and 1 section III, 2 section V, 26 BIS and 26 TER of the Agreement establishing the guidelines for the use of the Payment and Electronic Services Portal (PASE) of the Mexican Institute of Industrial Property, in the procedures indicated.

THE DIVISIONAL DIRECTOR OF PATENTS

NAHANNY CANAL REYES

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Tax | 1695 || MX / 2017/38534 | MX / a / 2013/002154 | PCT patent title | 1223 | GAGV | Page (s) | XekAHvK6HWwgToEMbQplrOHYDTc =

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Arenal No 550. Floor 1. Pueblo Santa María Tepepan, Xochimilco, 16020 Mexico City / 55) 53340700 ww gob. mx / impi

IIIIMH

MX / 2017/38534

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ΪΜΡΙ

NUTRITIONAL PRODUCTS THAT HAVE ---------------------------------------------- --------------------------------- tNDUMKIAL-IMPROVED ORGANOLEPTICS

Field of Invention

The present description relates to the nutritional products that comprise the pea protein hydrolysates. More particularly, the present disclosure relates to infant, pediatric and adult nutritional products comprising pea protein hydrolysates that have reduced immune reactivity and improved taste and organoleptic properties, including emulsion stability.

Background of the Invention

Pediatric, infant and adult nutritional liquids and powders manufactured comprising a specific selection of nutritional ingredients are well known and widely available, some of which may provide a single source of nutrition, while others may provide a supplemental source. These nutritional products include powders that can be reconstituted with water or other aqueous liquid, as well as concentrated, ready-to-drink nutritional liquids such as milk or protein-based emulsions or non-emulsified or substantially clear liquids. These nutritional liquids are especially helpful when formulated with the nutritional ingredients

<img file="MX347477B_D0004.tif" />

selected.

Many individuals, and particularly babies, including premature babies, experience intolerance to certain food ingredients in liquids and powders (formula intolerance). The terms "intolerance" and "formula intolerance" are used interchangeably herein. Intolerance is a reaction not associated with the immune system and can be evidenced by changes in the behavior or stool or eating pattern such as increased vomiting, an increased amount of stools, or more watery stools compared to individuals who tolerate nutritional ingredients well. . Intolerance is most often indicated by gastrointestinal symptoms (eg, vomiting, stool patterns, and gas) as well as behavioral characteristics (eg, acceptance of the formula, groaning, and crying).

Intolerance can be correlated with allergic-type reactions that some individuals, and especially infants, exhibit to certain nutritional compositions. These allergic-type reactions are associated with the immune system, and can be produced by the individual's sensitivity to the protein present (generally, cow's milk protein) in the nutritional product. These allergies or immune-related sensitivities frequently lead to skin, respiratory, or gastrointestinal symptoms such as vomiting and

<img file="MX347477B_D0005.tif" />

diarrhea.

Many individuals exhibiting cow's milk protein allergies or sensitivities may use nutritional products based on soy protein, and particularly, based on isolated soy protein.

Hydrolyzate-based formulas, some of which may be hypoallergenic, contain protein that has been hydrolyzed or divided into short peptide fragments and amino acids and are therefore almost immunologically inactive. Individuals who exhibit reactions to cow's milk and soy proteins will often not react to hydrolyzed formulas because their immune system does not recognize the hydrolyzed protein as the intact protein that causes their symptoms. Individuals who exhibit the immune system-associated reactions to nutritional compositions including cow's milk and / or soy proteins may also exhibit the non-immune system-associated reactions (formula intolerance), as described. previously.

Although products based on broadly hydrolyzed proteins or based on amino acid mixtures are generally acceptable to individuals who are allergic to cow's milk and soy proteins, they suffer from significant taste and organoleptic problems. Ajjnque many attempts have been made to avoid allergies and intolerance to

IMPI

INSTITUTE .MEXICANO

OF THE industrial PROPERTY formula through the preparation of the nutritional compositions when using the hydrolyzed proteins, and particularly the hydrolyzed soy proteins, the formulation of a nutritional product when using the hydrolyzed proteins without perceptibly damaging the proteins was previously known. organoleptic properties of the product, and particularly, the taste. For example, the use of hydrolyzed proteins commonly results in nutritional products that easily separate and / or precipitate out of solution, and are also bitter, salty, sour, and / or fruity in taste.

The present disclosure, therefore, is directed to nutritional products in the form of liquids, powders, nutritional bars, and other food products that comprise pea protein hydrolysates as the primary or sole source of the proteins. The pea protein hydrolyzate has such a degree of hydrolysis that the resulting nutritional products are physically stable over the shelf life and provide favorable organoleptic properties, while remaining substantially immunologically inactive. The nutritional product can also be used as a food source for individuals who are intolerant and / or allergic to proteins other than pea proteins and / or who suffer from malabsorption, maldigestion, or other gastrointestinal conditions.

Brief Description of the Invention tNSTHWO MEXICANA / a

OF THE PROPERTY

One embodiment is a hydrolyzed pea protein nutritional product that "Tlfíllü Lili yiydu — chr—" hydrolysis adjusted from about 7.0% to less than 19.0%, including from about 8.0% to about 16.0% , which includes between approximately 8% and approximately 13.5%, and also includes between approximately 12.0% and 16.0%. Additionally, in one embodiment, less than 5% of the proteins in the pea protein hydrolyzate have a molecular weight greater than 3 kDa. The product is in a form selected from the group consisting of a liquid beverage (eg, nutritional emulsions, substantially clear nutritional liquids, and gels), a powder, a nutritional product, a nutritional bar, and a nutritional supplement. Desirably the product is an infant formula.

Another embodiment is a nutritional product that includes a pea protein hydrolyzate that has less than 15,000 pg of the immunologically active pea antigen per 1 gram of protein.

Another embodiment is a method of making a nutritional product that comprises combining a protein, a fat, and a carbohydrate. The nutritional product includes a pea protein hydrolyzate having an adjusted degree of hydrolysis of between approximately 7.0% and less than 19.0% as its primary or sole source of protein. In one mode, less than

MEXICAN INSTITUTE - • Í'lJI

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5% of the proteins in the hydroxide of proteinaT have a molecular weight greater than 3 kDa. ΕΠ 'Utl'd Hiudalrdael, Pea Protein Hydrolyzate has less than 15,000 pg of immunologically active pea antigen per 1 gram of protein.

Nutritional products as described herein comprise pea protein hydrolysates as the sole or primary source of protein that not only provide a nutritional source for individuals who are intolerant and / or allergic to cow's milk proteins and / or soy proteins, but are also physically stable and / or have organoleptic advantages in each of the selected product forms. In many embodiments, described herein, the pea protein hydrolyzate is the only source of protein such that the nutritional product is hypoallergenic and is suitable for use in patients with food allergies.

It has now been discovered that this intolerance and / or allergic problems can be minimized or eliminated by formulating the nutritional product with a pea protein hydrolyzate having an adjusted degree of hydrolysis of between approximately 7.0% and less than 19.0%, which produces a specific molecular weight profile and antigen content. Specifically, the use of these pea protein hydrolysates has been found to have reduced activity. ''

ΙΜΡΪΟ ^ immunological. In addition, pro ^^^ - g ^ hydrolysates having an adjusted degree of hydrolysis of between about 7.0% and less than 19.0% provide improved taste and other improved organoleptic properties over nutritional products that include other sources of Hydrolyzed vegetable protein. Thus, by using the hydrolyzed pea protein of the present disclosure it is now possible to produce the nutritional products based on tolerated hydrolyzate with a pleasant taste and hypoallergenicity.

Brief Description of Drawings

Figure 1 is a diagram depicting the compositional comparison between the pea protein hydrolysates of the present disclosure and intact soy protein.

Detailed description of the invention

The nutritional products described herein comprise pea protein hydrolysates as a protein source. In many embodiments, nutritional products comprise pea protein hydrolysates as the sole protein source such that the food product is hypoallergenic and suitable for use in patients with food allergies. These and other essential features of nutritional products, as well as some of the many optional variations and additions, are described in detail hereinafter.

The term "hypoallergenic" as used herein,

IMPIOS unless otherwise specified, refers to nutritional FP that causes symptoms of an allergic reaction to food in less than 10% of individuals with a food allergy.

The term "substantially clear nutritional liquid" as used herein, unless otherwise specified, refers to a non-emulsified liquid or other similar liquid that has a visibly clear or translucent appearance, whose liquid can and will commonly have a dissolved or watery texture with a consistency similar to that of a clear juice and most commonly has a viscosity of less than about 25 cP as determined with a Brookfield viscometer at 22 ° C using a spindle # 1 at 60 rpm.

The term "spray dried nutritional powder" as used herein, unless otherwise specified, refers to a nutritional powder where most of the components, including the pea protein hydrolyzate, have been homogenized and subsequently subjected to a spray drying process during manufacturing. Additional ingredients can be added to the spray dried powder through dry mixing as long as at least the pea protein hydrolyzate has previously been homogenized and spray dried.

The term "adjusted degree of hydrolysis" as used herein, unless otherwise specified, refers to the

IMPI 0¾¾ degree of hydrolysis of a protefl ^ £_P^ fóe ^ í &amp; &lt;tú \ INDUSTRIAL '¾¿¾r ^ g ¢ *^,, generally the pea protein material, which has been adjusted or corrected to constitute the measurable amino-nitrogen content of the intact protein material. For example, if the intact and unmodified protein material has an amino-nitrogen content of 8.68 and a protein that has undergone a hydrolysis procedure has a degree of hydrolysis of 16.68, and the hydrolyzed protein has a degree of hydrolysis adjusted from 8.00.

The terms "fat" and "oil" as used herein, unless otherwise specified, are used alternatively to refer to lipid materials derived or processed from plants or animals. These terms also include synthetic lipid materials as long as such synthetic materials are suitable for oral administration to humans.

The term "human milk oligosaccharide" or "HMO" generally refers to a number of complex carbohydrates found in human milk. Monomers found in milk oligosaccharides commonly include D-glucose (CGL), D-galactose (Gal), N-acetylglucosamine (GIcNAC), L-fucose (Fue), and sialic acid [N-acetylneuraminic acid (NeuAc)]. The elongation of these monomers can be achieved with bonding

IMPI

INSTITUTO .MEXICANO ¿¿'ίζ ·· of the GIcNAc residues linked at the Gal residue bond followed by the addition to HiHnnai Ha rtai at a β-1-3 or β-1-4 bond. Most HMOs contain lactose at its lowering end. From these monomers, a large number of nuclear structures can be formed. Other variations can occur due to the binding of lactosamine, Fue, and / or NeuAc.

The term "non-perishable" as used herein, unless otherwise specified, refers to a food product that remains commercially stable after packaging and after being stored at 18-24 ° C for at least 3 months , including from about 6 months to about 24 months, and also including from about 12 months to about 18 months.

The terms "nutritional composition" or "nutritional product" as used herein are used interchangeably and, unless otherwise specified, refer to nutritional liquids, nutritional powders, nutritional bars, nutritional supplements, and any other product. nutritional as known in the art. The nutritional powders can be reconstituted to form a nutritional liquid, which comprises one or more of fats, proteins and carbohydrates and which is suitable for oral consumption by a human.

The term "nutritional liquid" as used herein,

WICKED

INSTITUTO MEXICaNC / A

OF THE PROPERTY ÜJ ».r3J., '' I ·¹Unless otherwise specified, refers to nutritional powders in ready-to-drink liquid form and ...... in concentrate, and to nutritional liquids prepared by reconstituting before use the nutritional powders described in the Present.

The term "baby" as used herein, unless otherwise specified, refers to a child 12 months or younger. The term "toddler" as used herein refers to a child 6 months to three years of age. The term "child" as used herein refers to a user from birth to 13 years of age. The term "premature baby" as used herein refers to a baby born before 36 weeks of gestation.

All percentages, parts, and ratios as used herein are by weight of the total composition, unless otherwise specified. All weights, as long as they pertain to the listed ingredients, are a function of active level and therefore do not include solvents or by-products that may be included in commercially available materials, unless otherwise specified.

All references to singular features or limitations in this description will include the corresponding plural feature or limitation, and vice versa, unless otherwise specified, or otherwise clearly understood by the context in which

WICKED

MEXICAN INSTITUTE a. ,. DI THE PROPERTY O ^ rXr.fV the reference is made. industrial

All combinations of the stages of the method q dal.— process as used herein can be performed in any order, unless otherwise specified or otherwise clearly understood by the context in which the combination is made referred.

Various embodiments of the nutritional products of the present disclosure may also be substantially free of any selected optional or essential ingredient disclosed herein, provided that the remaining nutritional product still contains all of the required ingredients or characteristics as described herein. . In this context, and unless otherwise specified, the term "substantially free" means that the selected nutritional product contains less than a functional amount of the optional ingredient, commonly less than about 1%, including less than about 0.5%, including less than about 0.1%, and which also includes zero percent, by weight of such selected or optional essential ingredient.

The nutritional products may comprise, consist of, or consist essentially of the essential elements of the products as described herein, as well as any additional or optional elements described herein or otherwise useful in the applications of the nutritional product.

Product shape

MEX1CANI INSTITUTE OF INDUSTRIAL PROPERTY

Nutritional compositions including pea protein hydrolyzate can be formulated and administered in any known or otherwise convenient oral product form. Any solid, liquid, or powder form, including combinations or variations thereof, is suitable for use herein, provided that such forms allow the safe and effective oral delivery to the individual of essential ingredients as specified. defined also in the present.

The nutritional compositions of the present disclosure include the pea protein hydrolysates as described herein. The compositions may include the pea protein hydrolysates as the sole protein source (i.e., the pea protein hydrolysates are 100% of the protein component of the composition) or may include the pea protein hydrolysates in combination with other hydrolyzed, partially hydrolyzed, or non-hydrolyzed (intact) proteins as described herein. Compositions include any product form that comprises the essential ingredients described herein, and that is safe and effective for oral administration. Nutritional compositions can be formulated to include only the essential ingredients described herein, or they can be modified with the optional ingredients to form '

IMPIOS,,, INSTITUTO MMICANC V; ·.; ft a number of different product forms. The nutritional ingredients of the present disclosure are preferably formulated as dietary product forms, which are defined herein as those embodiments that comprise the essential ingredients of the present disclosure in a product form that then contains at least one of fats, proteins, and carbohydrates, and preferably also contains the vitamins, minerals, or combinations thereof. In many embodiments, the composition will comprise the pea protein hydrolyzate as the sole source of protein in combination with the fats, carbohydrates, vitamins, and minerals to produce a hypoallergenic composition. A specific example is a hypoallergenic infant formula that includes 100% pea protein hydrolysates (as the protein source), fats, carbohydrates, vitamins, and minerals.

The nutritional compositions of the present disclosure, therefore, may include a variety of different product forms, including most conventional or otherwise known nutritional product forms, some of their non-limiting examples include confectionery, , cereals, food seasonings (for example, dressings, powders, sauces, jams, jelly, powdered milk replacer or coffee sweetener), pasta, baking or cooking materials (for example, flour, fats or oils, peanuts

<img file="MX347477B_D0006.tif" />

mixes for breading or baking), salty or seasoned snacks (for example, extruded, baked, fried), beverages (for example, coffee, juice, charred drink, non-charred drink, tea), snacks or meal replacement bars (for For example, Slimfast ™ bars, Ensure ™ bars, Zone Perfect ™ bars, Glucerna ™ bars), shakes, breakfast cereals, cheeses, gum products, salty or unsalted crunchy snacks (for example, chips, cookies , pretzel), sauces, baked goods (e.g. cookies, cakes, pies, biscuits, bread, bagels, toast cubes, fillings, dry mixes (e.g. muffin mixes, cookies, waffles, crepes, beverages), frozen desserts (e.g. For example, ice cream, popsicles, nougat bars, granita, frozen yogurt), pasta, processed meats (for example, banderillas, hamburgers, hot dogs, sausage, salami), pizza, flan, flavored or unflavored gelatin, refrigerated pasta (for example, cookies, bread, chocolate cake), yogurt or yogurt-based drinks, frozen yogurt, soups, vegetable-based burgers, and popcorn-based snacks.

The nutritional compositions of the present disclosure can also be formulated in product forms such as capsules, tablets, pills, oblong tablets, gels, liquids (eg, suspensions, solutions, emulsions, clear solutions), powders or other macroparticles, and so on. These

INSTITUTO MEXICANO 'faí-pcá, β product forms generally contain only^D^ &amp; u ^ (^ essential as described in nrpgpntp npcinnally in combination with other actives, processing aids, or other excipients in the dosage form.

Nutritional products include liquid and spray-dried powder forms, which include emulsified ready-to-eat liquids and reconstitutable spray-dried powders that can be diluted with water or other aqueous liquid to form a nutritional liquid before use.

Specifically, the nutritional compositions can be human milk fortifiers for premature babies intolerant to formulas based on cow's milk proteins or soy proteins, human milk fortifiers for babies allergic to cow's milk proteins or to milk proteins. soy proteins, premature baby formulas for premature babies intolerant to cow's milk protein or soy protein based formulas, o Preterm baby formulas for premature babies allergic to cow's milk proteins or soy proteins, infant formulas for babies intolerant to cow's milk protein or soy protein based formulas, Infants for babies allergic to cow's milk proteins or soy proteins, hypoallergenic formulas for babies with food allergies generally, subsequent infant formulas for

ΙΜΡΙ ^&gt; ^ infants intolerant to formulas based on protel $ SW I cow or soy protein, subsequent infantiiac formulas for infants allergic to formulas based on cow's milk protein or soy protein, formulas for toddlers for infants over six months of age who are intolerant to formulas based on cow's milk protein or soy protein, infant formulas for infants over six months of age who are allergic to soy protein or cow's milk protein-based formulas, nutritional drinks for children, and adults who are intolerant to nutritional products to cow's milk protein or soy protein based, nutritional drinks for children and adults who are allergic to nutritional products based on cow's milk protein or soy protein, nutritional drinks for geriatric patients who are intolerant to nutritional products based on cow's milk proteins or soy proteins, nutritional drinks for geriatric patients who are allergic to nutritional products based on cow's milk proteins or soy protein, nutritional sports drinks for people who are intolerant to nutritional products based on cow's milk protein or soy protein, nutritional sports drinks for people who are allergic to nutritional products based on cow's milk or soy proteins, nutritional sports drinks that

12 ^ 4 Ρ1

INSTITUTO MkXICANO / -i &lt;, A. ,. . ,,. - OF THE PROPERTY . Vwut ^ r '. ··· ^ use the hydrolyzed proteins with the^{, ND}| m ^ i improved organoleptic, the bars πιιίπΗ ^ ΐηη gu_? neon hydrolyzed proteins with improved organoleptic properties, semi-elemental formulas for the nutritional needs of patients suffering from malabsorption, maldigestion, and other gastrointestinal conditions, and formulas, bars, liquid or powdered protein supplements, or other food-based forms of hydrolyzed proteins designed for patients who require hydrolyzed protein to achieve their diets.

The nutritional compositions of the present disclosure, when formulated as a dietary product form, can potentially provide a single source or a source of supplemental nutrition for an individual. In this context, a single source of nutrition is one that can be administered once or multiple times each day to potentially provide an individual with all or substantially all of their fat, protein, carbohydrate, mineral, and vitamin needs per day or during planned administration period. A "supplemental nutrition source" is defined herein as a dietary source that does not provide an individual with a potentially unique source of nutrition.

Liquid drinks

Liquid drinks include concentrated and ready-to-drink liquids. Non-limiting examples of forms. . INSTITUTO MEXICANO β liquid products suitable for the «TS ^^^ a include liquid infant formulas (which include both

II | | •"..read_w, --- | ready-to-drink formulations such as concentrated formulations), liquid infant formulations (which include both ready-to-drink formulations and concentrated formulations), liquid human milk fortifiers (which include both ready-to-drink formulations and concentrated formulations), snack and meal replacement products, hot or cold drinks, carbonated or non-carbonated drinks, juices or other acidified beverages, milkshakes, coffees, teas, enteral feeding compositions, and so on. These liquid compositions are very commonly formulated as suspensions or emulsions, but they can also be formulated in any other convenient way such as clear liquid solutions, liquid gels, and the like.

The liquid nutritional beverages of the present disclosure can be used as nutritional beverages for premature babies, infants, toddlers, children, and adults, including geriatric patients, who are intolerant or allergic to milk protein-based nutritional products. beef and / or soy protein. Liquid beverages can also be used as semi-elemental formulas for the nutritional needs of patients with malabsorption, maldigestion, and other gastrointestinal conditions.

A. Nutritional emulsions

IMPI

INSTITUTO MEXICANO, ___________. „._:___. ° £ Ló WPIMIAO, / Λ ^ · “ΰϊ2Είΐ

Nutritional emulsions comprise the pea protein as the source protein, and are aqueous emulsions that additionally comprise fats and carbohydrates. These emulsions are fluid or drinkable liquids at about 1 ° C to about 25 ° C and are commonly in the form of oil / water, water / oil, or complex aqueous emulsions, although such emulsions are commonly in the form of oil / water emulsions. having a continuous aqueous phase and a discontinuous oil phase.

Nutritional emulsions can and are commonly non-perishable. Nutritional emulsions commonly contain up to about 95% by weight of water, which includes from about 50% to about 95%, also includes from about 60% to about

90%, and also includes from about 70% to about 85%, by weight of the water of the nutritional emulsions.

Nutritional emulsions can be formulated with sufficient classes and amounts of nutrients to provide a single, primary, or complementary source of nutrition, or to provide a specialized nutritional emulsion for use in individuals suffering from specific diseases or conditions. For example, nutritional emulsions can be produced for use as infant formulas, infant

Subsequent Mexican IMPie ^ Msmvro and for young children, formulafé ^ ii® ^ similar for users who are intniprantpq n allergic to nutritional products based on cow's milk protein and / or soy protein. In another embodiment, the emulsions can be used as a hypoallergenic nutritional emulsion by individuals who have general food allergies. These nutritional emulsions, therefore, can have a variety of product densities, but very commonly have a density greater than about 1.03 g / ml, which includes greater than about 1.04 g / ml, which includes greater than about 1.055 g / ml. , which includes from about 1.06 g / ml to about 1.12 g / ml, and which also includes from about 1.085 g / ml to about 1.10 g / ml.

Nutritional emulsions can have a caloric density adapted to the nutritional needs of the end user, although in most cases the emulsions comprise approximately 100 to approximately 500 kcal / 240 ml, which include approximately 150 to approximately 350 kcal / 240 ml. , and that also include about 200 to about 320 kcal / 240 mi.

In an alternative embodiment, the Nutritional Emulsion is a low calorie nutritional emulsion and has a caloric density of approximately 6.5 kcal / fl oz (52.8 kcal / 240 ml). In another embodiment, the nutritional emulsion has a caloric density of approximately 7.1 kcal / fl oz (57.6

IMPI kcal / 240 mi). In one embodiment, the emulsion

INDUSTRIAL caloric density of approximately 7.7 kcal / fl oz (62.4 kcal / ml).

The nutritional emulsion may have a pH ranging from about 3.5 to about 8, but is very advantageously in a range from about 4.5 to about 7.5, including from about 5.5 to about 7.3, including from about 6.2 to about 7.2.

Although the serving size for nutritional emulsion may vary depending on a number of variables, a common serving size ranges from about 100 to about 300 ml, including from about 150 to about 250 ml, and including from about 190 ml to approximately 240 mi.

In one embodiment, the nutritional emulsion can be used as a human milk fortifier. In such modalities, a single common serving size includes approximately 5.0 ml.

B. Substantially clear liquid beverages

The substantially clear nutritional liquids of the present disclosure are dilute liquids that comprise at least the protein, at least a protein portion which is the pea protein hydrolyzate, and the carbohydrates as will be discussed later. Substantially clear nutritional liquids are substantially free of

IMPI fat; that is, the liquids are after YoáMwfo ^ AD

- 11 · ι · ιι ·· ιι IB raw materials or fat added at low concentrations as an aid in the manufacture of the liquid. In this context, the term "fat-free" means that the liquid commonly contains less than 1.0%, more commonly less than 0.5%, and very commonly less than 0.1%, which includes the zero weight percent fat of the nutritional liquid. . These substantially clear nutritional liquids are flowable or drinkable liquids at a temperature of about 1 ° C to about 25 ° C.

Substantially clear nutritional liquids can be and are commonly non-perishable. Liquids commonly contain up to about 95% by weight of water, which includes from about 50% to about

95%, which also includes from about 60% to about 90%, and also includes from about 70% to about 85% by weight of water of the substantially clear nutritional liquid.

Substantially clear nutritional liquids can be formulated with sufficient classes and amounts of nutrients to provide a supplemental source of nutrition, or to provide a specialized nutritional liquid for use in individuals suffering from specific diseases or conditions. For example, in one embodiment, the nutritional fluid

INSTITUTO MEXICANO substantially transparent is a depoVówa drink ^^ by users who are intolerant or allergic to nutritional ^ ° pmriuHns based on cow's milk protein and / or soy proteins. In another embodiment, the substantially clear nutritional liquid is a sports drink that includes the hydrolyzed protein with the enhanced organoleptic properties. These substantially clear nutritional liquids, therefore, can have a variety of product densities, but very commonly have a density greater than about 1.040 g / ml, which includes 1.06 g / ml to 1.12 g / ml, and which also includes from about 1.085 g / ml to about 1.10 g / ml.

Substantially clear nutritional liquids can have a caloric density adapted to the nutritional needs of the end user, although in most cases the liquids comprise from about 90 to about 500 kcal / 240 ml, including from about 150 to about 350 kcal / 240 mi, including about 180 to about 350 kcal / 240 mi and also including about 250 to about 320 kcal / 240 mi. In other embodiments, the substantially clear nutritional liquids comprise from about 90 to about 500 kcal / 480 ml, including from about 150 to about 350 kcal / 480 ml, and also including from about 250 to about 320 kcal / 480 ml.

IMPI

INSTITUTE MF.XtCAN &lt;&gt;

OF INDUSTRIAL PROPERTY

Substantially clear nutritional liquids have a pH ranging from about 2.8 to about 4.6, including from about 2.9 to about 4.2, and also including from about 3.1 to about 3.9.

Although the serving size for the substantially clear nutritional liquid can vary depending on a number of variables, a common serving size ranges from about 100 to about 591 ml, which includes from about 150 to about 250 ml, which includes from about 190 ml. at approximately 240 mi. Some specific serving sizes for the Substantially Clear Nutritional Liquid include 8.1 oz. (240 ml), 10 oz. (296 ml), and 16 oz. (480 ml).

Food powders

Nutritional powders comprise pea protein hydrolysates as the primary or sole source of the proteins and are commonly in the form of flowable or substantially flowable particulate compositions, or at least particulate compositions. Particularly suitable product forms include spray dried, bonded or dry mixed powder compositions. Compositions can be scooped out and easily measured with a spoon or other similar device, where

<img file="MX347477B_D0007.tif" />

IMPI MEXICAN INSTITUTE. . . DELA PROPERTY compositions can be easily reconstituted pO'P ^ h¹ provided with a suitable aqueous liquid, COmmonmount water ·, · - to form a nutritional formulation for immediate oral or enteral use. In this context, "immediate" use generally means within a period of about 48 hours, more commonly within a period of about 24 hours, preferably immediately after reconstitution.

Nutritional powders can be formulated with sufficient classes and amounts of nutrients to provide a single, primary, or complementary source of nutrition, or to provide a specialized nutritional powder for use by individuals suffering from specific diseases or conditions. In a specific embodiment, the nutritional powder can be formulated for use with individuals who are intolerant or allergic to nutritional products based on cow's milk proteins and / or soy proteins. In another embodiment, the emulsions can be used as hypoallergenic nutritional emulsions by individuals suffering from general food allergies.

The nutritional powders can be reconstituted with water before use at a caloric density adapted to the nutritional needs of the end user, although in most cases the powders are reconstituted with water to form compositions comprising from about 100 to about 500 kcal / 240 mi, including

IMPI

INSTITUTO MEXICANO -.ZS * A

PROPERTY 2i · 'J about 150 to about 35p kcal / ^ w ^ l also include from about ^ nn to about 320 kcal / 240 mi.

In an alternative embodiment, the nutritional powder can be reconstituted with water to form a low calorie composition having a caloric density of approximately 52.8 kcal / 240 ml. In another embodiment, the nutritional powder has a caloric density of approximately 57.6 kcal / 240 ml. In one embodiment, the nutritional powder has a caloric density of approximately 62.4 kcal / 240 ml.

Although the serving size for reconstituted nutritional liquid may vary depending on a number of variables, a common serving size ranges from about 100 to about 300 ml, which includes from about 150 to about 250 ml, which includes from about 190 ml to approximately 240 mi.

In a specific embodiment, the reconstituted nutritional liquid can be used as a human milk fortifier. In such embodiments, a single common serving size includes about 0.9 grams of powder per about 100 to about 300 ml of water.

Other solid nutritional products

The nutritional products can alternatively be in the form of solid nutritional bars and solid food products. For example, nutritional products can be

IMPIOS MEXICAW instiivto formulate as bars, sticks, or cookies, breads ^¹! ^^^^ baked products. * ••• ΜΙβίβΒ * «i ·! -,_L ,____

In another embodiment, the nutritional compositions of the present disclosure can also be formulated into product forms such as capsules, tablets, pills, lozenges, and gels. These product forms generally contain only the essential ingredients as described herein, optionally in combination with other actives, processing aids, or other excipients in the dosage form.

Baby formula

When the nutritional product is an infant formula, the nutritional product can be a liquid or solid nutritional product as described above. The liquid or solid infant formula products of the present disclosure may have any caloric density convenient for the target babies, or they may provide such a density upon reconstitution from a powder modality or upon dilution from a liquid concentrate modality. For example, most common caloric densities for the infant formula modalities of the present disclosure are generally at least about 19 kcal / fl oz (660 kcal / liter), more commonly about 20 kcal / fl oz (675 -680 kcal / liter) to about 25 kcal / fl oz (820 kcal / liter), most commonly about 20 kcal / fl oz (675-680

INSTITUTO.M BXICANo YES kcal / liter) to approximately 24 kcal / fl oz

Generally, 22-24 kcal / ounce liquid formulas are very commonly used in premature or low birth weight babies, and 20-21 kcal / fl oz (675-680 to 700 kcal / liter) 5 formulas are used more frequently in babies who completed their gestation.

In an alternative embodiment, infant formula is a low calorie infant formula and has a caloric density of approximately 6.5 kcal / fl oz (220 kcal / liter). In one embodiment, infant formula has a caloric density of approximately 7.1 kcal / fl oz (240 kcal / liter). In one embodiment, infant formula has a caloric density of approximately 7.7 kcal / fl oz (260 kcal / liter).

Although the total concentrations or amounts of fat, protein, and carbohydrates may vary depending on the product form (i.e., powder, drinking liquid, or concentrated liquid) and the target dietary needs of the intended user, such concentrations or amounts very commonly fall within one of the following incorporated ranges, including 20 of any other essential fat ingredient, protein, and or carbohydrate as described herein.

For liquid infant formula products, carbohydrate concentrations most commonly range from about 5% to about 40%, including from about 7% to about 30%, including from

ΙΜΡΙ ^&gt;

INSTITUTO MEXICANO • E LA FROFIEDAD approximately 10% to approximately 25% 6 ^ 1 ^^^^ 0 ^ 345 + nutritional product; graea eoailon concentrations most commonly from about 1% to about 30%, including from about 2% to about 15%, and also including from about 4% to about 10% by weight of the nutritional product; and protein concentrations most commonly range from about 0.5% to about 30%, including from about 1% to about 15%, and also including from about 2% to about 10% by weight of the food product.

In an alternative embodiment, the liquid infant formula product is a low calorie liquid infant formula and includes less than about 5% by weight of carbohydrates, about 1% by weight of fat, and about 0.5% by weight of protein.

The level or amount of carbohydrates, fats, and / or proteins in liquid nutritional products can also be further or alternatively characterized as a percentage of total calories in nutritional compositions as explained in the following table. These macronutrients for the liquid nutritional products of the present disclosure are most commonly formulated within any of the caloric ranges (modes AF) described in the following table (each numerical value is preceded by the term "about").

• κ »* ♦

<td colspan="6"></td>

<td>Nutrients (% of total calories)</td><td>Mode A</td><td>Mode B</td><td></td><td></td><td> &amp;</td>

<td></td><td></td><td></td><td></td><td></td><td></td>

<td>Carbohydrate</td><td> 0-98</td><td colspan="2"> 2-96</td><td> 10-75</td><td></td>

<td>Protein</td><td> 0-98</td><td colspan="2"> 2-96</td><td> 5-70</td><td></td>

<td>Grease</td><td> 0-98</td><td colspan="2"> 2-96</td><td> 20-85</td><td></td>

<td></td><td>Mode D</td><td colspan="2">Mode E</td><td>Mode F</td><td></td>

<td>Carbohydrate</td><td> 30-50</td><td colspan="2"> 25-50</td><td> 25-50</td><td></td>

<td>Protein</td><td> 15-35</td><td colspan="2"> 10-30</td><td> 5-30</td><td></td>

<td>Grease</td><td> 35-55</td><td colspan="2"> 1-20</td><td> 2-20</td><td></td>

For example, liquid infant formulas (both ready-to-drink and concentrated liquids) include those modalities in which the protein component may comprise from about 7.5% to about 25% of the caloric content of the formula; the carbohydrate component can comprise from about 35% to about 50% of the total caloric content of the infant formula; and the lipid component can comprise from about 30% to about 60% of the total caloric content of the infant formula. These ranges are provided as examples only, and are not intended to be limiting. Additional convenient ranges are noted in the following table (each numerical value is preceded by the term "about").

<img file="MX347477B_D0008.tif" />

<td>Nutrients (% of total calories)</td><td>Mode G</td><td>Mode H </td><td></td>

<td>Carbohydrates:</td><td> 20-85</td><td> 30-60</td><td> 35-55</td>

<td>Lipid:</td><td> 5-70</td><td> 20-60</td><td> 25-50</td>

<td>Protein:</td><td> 2-75</td><td> 5-50</td><td> 7-40</td>

When the nutritional product is a powdered infant formula, the protein component is present in an amount of from about 5% to about 35%, including from about 8% to about 12%, and including from about 10% to about 12%. by weight of the powdered nutritional product; the fat component is present in an amount from about 10% to about 35%, including from about 25% to about 30%, and including from about 26% to about 28% by weight of the powdered nutritional product; and the carbohydrate component is present in an amount of from about 30% to about 85%, including from about 45% to about 60%, including from about 50% to about 55%, by weight of the powdered infant formula.

The amount or total concentration of fats, carbohydrates, and proteins, in the powdered nutritional products of the present description can vary considerably depending on the selected formulation and the needs.

<img file="MX347477B_D0009.tif" />

<img file="MX347477B_D0010.tif" />

The user's dietary or medical review of additional convenient macronutrient concentrations are explained below. In this context, the total amount or concentration refers to all the sources of fat, carbohydrates, and proteins in the powdered product. For powdered infant formulas, such total amounts or concentrations are very commonly formulated and preferably fall within any of the incorporated ranges described in the table below (all numbers are preceded by the term "about").

<td>Nutrient (% of calories)</td><td>Mode A</td><td>Mode B</td><td>Mode C</td>

<td>Carbohydrate</td><td> 20-85</td><td> 30-60</td><td> 35-55</td>

<td>Grease</td><td> 5-70</td><td> 20-60</td><td> 25-50</td>

<td>Protein</td><td> 2-75</td><td> 5-50</td><td> 7-40</td>

Pea protein hydrolyzate

Nutritional products comprise pea protein hydrolyzate as the primary or sole source of protein. In nutritional modalities where a hypoallergenic composition is desired (eg, a hypoallergenic infant formula), the nutritional composition will include 100% pea protein hydrolyzate, by weight of the protein component. The pea protein hydrolysates used in the products of the present disclosure have been found to

IMPI INSTITUTO MEXICANO show a disWílí ^ S ^? improved taste and organoleptic properties compared to other vegetable protein hydrolysates, such as soy proteins, as well as cow's milk proteins. For example, as shown in Figure 1, pea protein hydrolysates for use in the nutritional products of the present disclosure have reduced levels of phytoestrogens (i.e., genistein and daidzein, which are isoflavones), aluminum, manganese, and phytates, which therefore improves the nutrient profile of the final nutritional product. Furthermore, as described herein, the pea protein hydrolyzate has a reduced antigen content and / or a reduced immune reactivity. Therefore, pea protein hydrolysates provide a good source of protein for nutritional products that are designed for individuals who are intolerant and / or allergic to cow's milk proteins and / or soy proteins, as well as they meet the nutritional needs for individuals suffering from malabsorption, maldigestion, and other gastrointestinal conditions. These nutritional products also have improved flavor and emulsion stability.

Pea protein hydrolysates may be the only protein source in the nutritional products of the present disclosure. Alternatively, additional protein sources, as will be described below, can be used in

IMPI the products in combination with the hydroized ones^Dtte'a0T © toxin ^^ ”Ndu &lt;TRIAL pea to provide total protein. Accordingly, the pea protein hydrolyzate provides at least 50% of the total protein in the nutritional product, including from about 50% to about 100% of the total protein, including from about 60% to about 95%. of the total protein, which includes from about 70% to about 90% of the total protein, and which also includes from about 75% to about 85% of the total protein in the nutritional product. As noted herein, additional protein sources may include partially hydrolyzed, hydrolyzed, and / or non-hydrolyzed (intact) proteins.

In particular, it has been surprisingly discovered that by controlling the hydrolysis of a source of pea protein, such as pea protein isolate or pea protein concentrate, to a particular degree, pea protein hydrolysates can be produced with a reduced content of antigens, and therefore with reduced immune reactivity, without adversely affecting the taste qualities of the hydrolyzate. The pea protein hydrolysates described herein are superior to previously known soy proteins in that they have: (1) undetectable levels of isoflavones; (2) decreased concentrations of phytate, manganese, and aluminum; (3) better taste and organoleptic profiles; and (4) not genetically modified.

IMPI

MEXICAN INSTITUTE

OF THE INDUSTRIAL PROPIF.1MD

<img file="MX347477B_D0011.tif" />

Generally, pea protein hydrolysates for use in the nutritional products of the present disclosure are produced by: (1) blending a pea protein product that has at least 65% protein (by weight of dry matter) and water to form a mixture with a protein content of up to about 20%, preferably up to about 12%; (2) hydrolyze the mixture; and (3) filtering the hydrolyzed mixture to obtain the protein hydrolyzate.

The pea protein product used as raw material in step (1) can be any pea protein product, provided that its protein content is at least 65% by weight of dry matter, that is , pea protein concentrates (with a protein content of more than 70% by weight of dry matter), or pea protein isolates (with a protein content of more than 90% by weight of dry matter). Commercially available pea protein concentrates and isolates are well known in the art.

The pea protein product is mixed with water to form a suspension with a protein content of up to about 20%, preferably up to about 12%. In one or more embodiments, the mixture can be heated prior to hydrolysis to obtain further denaturation. For example, in one mode, the

<img file="MX347477B_D0012.tif" />

<img file="MX347477B_D0013.tif" />

INSTIT1ITO MEXICANO method also includes warming up.^£ temperature of plus 60 ° C before i =&gt; hydrolysis Most conveniently, the mixture can be heated to a temperature of from about 70 ° C to about 90 ° C for a period of from about 2 minutes to about 20 minutes before hydrolysis. In this way, the protein is effectively denatured, and therefore the subsequent hydrolysis will generally proceed in a more rapid manner. Furthermore, by heating the mixture, microbial stability during hydrolysis, which can last several hours, is effectively ensured.

Once formed, and optionally heated, the temperature of the suspension is preferably adjusted and maintained at between about 40 ° C and about 70 ° C, including from about 50 ° C to about 60 ° C, before and during hydrolysis reaction according to methods known in the art. Certainly, other heating steps and / or other temperatures can be used as is known in the art.

The hydrolysis of the protein-containing suspension can be carried out enzymatically or non-enzymatically. In one embodiment, the protein-containing suspension is hydrolyzed to have an adjusted degree of hydrolysis of between about 7.0% and less than 19.0%, including between about 8.0% and about 16.0%, including between

IMPI

INSTITUTO MtXICANC approximately 8.0% and approximately 13 includes between approximately 12.0% v approximately

14.00%, and which also includes approximately 14.0% and a molecular weight distribution and antigen content as explained below. Such hydrolysis, in one embodiment, can be carried out proteolytically as is known in the art.

In another embodiment, the hydrolysis is carried out with one or more endo- and / or exo-peptidase enzymes of non-specific action. Convenient non-specific endo- and / or exo-peptidase enzymes include, for example, enzymes derived from an Aspergillus strain, particularly from an Aspergillus niger strain, an Aspergillus oryzae strain, or an Aspergillus soyae strain, or a Bacillus strain, particularly a Bacillus amiloliquefaciens strain, a Bacillus lentus strain, a Bacillus licheniformis strain or a Bacillus subtilis strain.

Additional convenient endopeptidase enzymes for use in hydrolysis step (2) include, for example: (i) a glutamyl endopeptidase (EC 3.4.21.19); (Ii) a lysylendopeptidase (EC 3.4.21.50); (iii) a leucyl endopeptidase (EC 3.4.21.57); (iv) a glycyl endopeptidase (EC 3.4.22.25); (v) a prolyl endopeptidase (EC 3.4.21.26); (vi) trypsin (EC 3.4.21.4) or a trypsin-like endopeptidase (lysine / specific arginine); or (vii) a peptidyl-Asp metalloendopeptidase (EC 3.4.24.33). Additional convenient exopeptidase enzymes include, for example, a carboxypeptidase, for example: (i) a proline carboxypeptidase (EC 3.4.16.2);

(ii) an industrial carboxTpep ^

<img file="MX347477B_D0014.tif" />

3.4.17.1); (iii) a carboxypeptidase B (EC 3.4.17.2); (iv) a carboxypeptidase C (EC 3.4.16.5); (v) a carboxypeptidase D (EC

3.4.16.6); (vi) a lysine carboxypeptidase (arginine) (EC

3.4.17.3); (vii) a glycine carboxypeptidase (EC 3.4.17.4); (viii) an alanine carboxypeptidase (EC 3.4.17.6); (ix) a glutamate carboxypeptidase (EC 3.4.17.11); (x) a peptidyl dipeptidase A (EC 3.4.1 5.1); or (XI) a peptidyl dipeptidase (EC 3.4.15.5).

The exact amount of enzyme added to the suspension to obtain the desired characteristics of pea protein hydrolysates will vary depending on characteristics such as: (1) the enzyme or enzyme system used; (2) the final desired degree of hydrolysis: and / or (3) the final desired molecular weight distribution. Because these are the known parameters, one skilled in the art can easily determine the appropriate conditions to achieve the desired characteristics of the final pea protein hydrolyzate. Commonly, the enzyme or enzyme system is added to the suspension in amounts ranging from about 10 mg to about 5000 g, including from 35 mg to about 700 g per 35 kg of protein in the suspension.

The hydrolysis reaction will commonly run for a desired length of time to obtain the desired degree of hydrolysis and / or the desired molecular weight profile. Commonly, the hydrolysis reaction will continue for a period of

WICKED

Mexican INSTITUTE.

. ,,. _. . . Dt THE PROMITY Veia ^ XíiLsfS about 15 minutes to about 24 ^ 4 ^ 13 ^ 5, ^ 3¾ ^ includes about 30 minutes to apiwñmnrun ™ rq_?minutes, and that also includes from about 45 minutes to about 120 minutes. Again, the exact amount of time required for the hydrolysis reaction will depend on the specific characteristics as noted above, but can be readily determined by one of skill in the art.

In other embodiments, the protein-containing suspension can be hydrolyzed using non-enzymatic means, such as by chemical and / or mechanical (physical) hydrolysis. Such a technique was also well known in the art.

Once the pea protein material has been hydrolyzed to the desired degree, the hydrolysis reaction is stopped, for example, by deactivating the enzyme or enzyme system, or by other conventional means. In one embodiment, deactivation of the enzyme or enzyme system is accomplished with heat treatment. This deactivation is especially well suited in the case where the pH of the final pea protein hydrolyzate is relatively high.

In accordance with established practice, the enzyme preparation can be conveniently deactivated by increasing the temperature of the incubation suspension to a temperature where the enzymes are deactivated, for example at greater than about 70 ° C, and conveniently at a temperature of about 90 'C.

Alternatively, the enzymes can be inactivated by suitable acid treatment. This deactivation is especially well suited in cases where the pH of the final pea protein hydrolyzate will be relatively low. For example, enzymes can be deactivated by lowering the pH of the suspension to a value where the enzymes are deactivated, for example below about 4.0.

Optionally, after the hydrolysis reaction has stopped, the method of preparation may further comprise treating the suspension with activated carbon for a convenient period at a temperature of about 50 ° C and about 70 ° C, for example, in an amount which generally corresponds to between about 1 and about 5% carbon, calculated with reference to the dry matter content. This optional carbon step can enhance the color of the final pea protein hydrolyzate and also further reduce the off-taste of the pea protein hydrolyzate.

Finally, the hydrolyzed suspension is filtered using a convenient conventional filtration process to obtain the pea protein hydrolyzate. For example, in one embodiment, a filtration is carried out by nanofiltration at a temperature between 50 ° C and 70 ° C and / or by evaporation, - -------. ._____________

IMPIOS after which the concentrate is collected ^ t ^^ ft ^^

INDUSTRIAL of pea protein hydrolyzate. By means of nanofiltration, a desalination can be carried out through the appropriate selection of the membrane. Evaporation has the advantage of obtaining a high content of dry matter in the concentrate before drying.

In a specific embodiment, the pea protein hydrolyzate, once obtained by the aforementioned filtration process, is subjected to a second filtration process, such as an ultrafiltration process, to provide a convenient molecular weight distribution for use in semi-elemental and related nutritional products. The ultrafiltration unit may be a hollow fiber membrane, such as the unit commercially available under the trademark Diaflo® from Amicon Corporation and / or under the trademark Romicon® from Romicon Corporation, having the desired nominal weight values. molecular limit. The hollow fiber membranes are filled into a filter cartridge to allow flow through them, whereby the suitably small molecules pass through the membrane, the effluent rate of the hydrolyzate is dependent on the pressure drop through membrane, and are extracted in solution in a collection area, where the rest of the solution, It contains the highest molecular weight protein hydrolyzate that passes directly through the fibrous tubes, for recycling into the separate collection zone reactor, as desired.

<td>In addition, this</td><td>second stage</td><td colspan="3">optional filtration</td><td>can</td>

<td>reduce levels</td><td colspan="5">of phytate resulting in the hydrolyzate of</td>

<td colspan="3">pea protein. In particular, the content</td><td>from</td><td colspan="2">phytate is</td>

<td colspan="3">reduces in pea protein hydrolysates</td><td>by</td><td>the</td><td>less</td>

<td>approximately</td><td>10%, what</td><td>includes by</td><td>the</td><td></td><td>less</td>

<td>approximately</td><td>15%, what</td><td>includes by</td><td>the</td><td></td><td>less</td>

<td>approximately</td><td>20%, what</td><td>includes by</td><td>the</td><td></td><td>less</td>

<td>approximately</td><td>25%, that</td><td>includes by</td><td>the</td><td></td><td>less</td>

<td>approximately</td><td>30%, what</td><td>includes by</td><td>the</td><td></td><td>less</td>

<td>approximately</td><td>35%, that</td><td>includes by</td><td>the</td><td></td><td>less</td>

<td>approximately</td><td>40%, and what</td><td>includes by</td><td>the</td><td></td><td>less</td>

<td colspan="2">about 45% or more.</td><td></td><td></td><td></td><td></td>

<td colspan="3">Pea protein hydrolysates for</td><td colspan="2">the use</td><td>in the</td>

<td colspan="3">nutritional products described herein are</td><td colspan="3">i prepare the</td>

use the methods described above and have the following characteristics. Exemplary pea protein hydrolysates made according to the above methods include those having a molecular weight distribution of less than 5% that is greater than 5kD.

The pea protein hydrolysates produced according to the methods described above additionally have reduced immunological reactivity. In one embodiment, the immune reactivity of the pea protein hydrolyzate is tested by using

Enzyme-Linked Immunosorbent) and is expressed as the amount of active immune antigen times the amount of protein. For example, one gram of pea protein hydrolyzate prepared according to the methods described herein will commonly include less than about 15,000 pg of immunologically active pea antigen, which includes less than about 10,000 pg of pea antigen.

<td colspan="2">pea immunologically</td><td>active,</td><td colspan="2">what includes</td><td>less</td><td>from</td>

<td>approximately</td><td>7,500 pg</td><td>from</td><td>antigen</td><td>from</td><td colspan="2">pea</td>

<td>immunologically</td><td>active,</td><td>that</td><td>It includes</td><td colspan="2">less</td><td>from</td>

<td>approximately</td><td>5,000 pg</td><td>from</td><td>antigen</td><td>from</td><td colspan="2">pea</td>

<td>immunologically</td><td>active,</td><td>that</td><td>It includes</td><td colspan="2">less</td><td>from</td>

<td>approximately</td><td>2,500 pg</td><td>from</td><td>antigen</td><td>from</td><td colspan="2">pea</td>

<td>immunologically</td><td>active,</td><td>that</td><td>It includes</td><td colspan="2">less</td><td>from</td>

<td>approximately</td><td>500 pg</td><td>from</td><td>antigen</td><td>from</td><td colspan="2">pea</td>

<td>immunologically</td><td>active,</td><td>that</td><td>It includes</td><td colspan="2">less</td><td>from</td>

<td>approximately</td><td>250 pg</td><td>from</td><td>antigen</td><td>from</td><td colspan="2">pea</td>

<td>immunologically</td><td>active,</td><td>that</td><td>It includes</td><td colspan="2">less</td><td>from</td>

<td>approximately</td><td>100 pg</td><td>from</td><td>antigen</td><td>from</td><td colspan="2">pea</td>

<td>immunologically</td><td>active,</td><td>that</td><td>It includes</td><td colspan="2">less</td><td>from</td>

<td>approximately</td><td>50 pg</td><td>from</td><td>antigen</td><td>from</td><td colspan="2">ch charo</td>

<td>immunologically</td><td>active, and</td><td>that</td><td>includes the</td><td colspan="2">hydrolyzed</td><td>from</td>

pea protein that is substantially free of the immunologically active pea antigen.

Pea protein hydrolyzate

<img file="MX347477B_D0015.tif" />

The methods described above also have an immune response index of less than 2.5 log units of IRI, which includes less than 2.4 log units of IRI, which includes less than 2.3 log units of IRI, which includes less than 2.2 log units of IRI, which includes less than

2.1 logarithmic units of IRI, and which also includes less than

2.0 logarithmic units of IRI.

Protein

The nutritional products of the present disclosure may optionally comprise an additional protein source or sources in addition to the pea protein hydrolyzate. Any additional protein source that is suitable for use in oral nutritional products and that is compatible with the essential elements and characteristics of such products is suitable for use in combination with pea protein hydrolysates.

When used in a liquid beverage, the total protein concentration in the nutritional liquid beverage can range from about 0.5% to about 40%, including from about 0.5% to about 30%, including from about 1% to about 15%. , and which also includes from about 1% to about 10%, and which also includes from about 1% to about 7%, by weight of the liquid nutritional beverages. In a mode

Specific IΜ ΡI, the protein is present in an INDUSTRIAL in an amount of approximately 6.0% by weight of nutritional emulsion.

When used in solid nutritional products, such as nutritional powders and bars, and other food products, the total protein concentration in solid nutritional products can range from about 1.0% to about 50%, including from about 10% to about 50%. %, and which also includes from about 10% to about 30%, by weight of the solid food product. In a specific embodiment, the protein is present in a spray dried nutritional powder in an amount of about 19%, by weight of the spray dried nutritional powder.

Non-limiting examples of additional convenient protein or sources thereof for use in nutritional products include hydrolyzed, partially hydrolyzed or non-hydrolyzed proteins or sources of protein, which can be derived from any known source or from otherwise convenient as milk (eg, casein, whey), animal (eg, meat, fish), cereal (eg, rice, corn), vegetable (eg, soy), or combinations thereof. Non-limiting examples of such proteins include milk protein isolates, milk protein concentrates as described herein, ί Μ Ρϊ casein protein isolates, whey protein, calcium casein, whole cow's milk, Partially or completely skim milk, soy protein isolates, soy protein concentrates, etc.

Carbohydrates

The nutritional products may further comprise any carbohydrate that is convenient for use in an oral nutritional product and that is compatible with the essential elements and characteristics of such products. Carbohydrate concentrations in liquid nutritional beverages, for example, can range from about 5% to about 40%, including from about 7% to about 30%, including from about 10% to about 25%, by weight of the nutritional liquid drink. In a specific embodiment, carbohydrates are present in a nutritional emulsion in an amount of about 10.2%, by weight of the nutritional emulsion.

Carbohydrate concentrations in solid nutritional products can range from about 10% to about 90%, including from about 20% to about 80%, further including from about 40% to about 60%, by weight of the solid nutritional product. In a specific embodiment, the carbohydrates are present in a spray dried nutritional powder in an amount of about 58%, by weight of the

WICKED

INSTITUTO MEXICANO spray dried nutritional powder. ^M^ indwtrml

Non-limiting examples of convenient carbohydrates or sources thereof for use in the nutritional products described herein may include maltodextrin, hydrolyzed or modified corn starch, glucose polymers, corn syrup, solids. from corn syrup, carbohydrates derived from rice, carbohydrates derived from peas, carbohydrates derived from potato, tapioca, sucrose, glucose, fructose, lactose, high fructose corn syrup, honey, sugar alcohols (eg, maltitol, erythritol, sorbitol), artificial sweeteners (eg, sucralose, acesulfame potassium, stevia), and combinations thereof. A particularly desirable carbohydrate is a low dextrose equivalent (DE) maltodextrin.

Fats

Nutritional products may additionally comprise fats, most commonly as emulsified fat. Fats may be present in liquid nutritional beverages in an amount from 0% to about 30%, including from 1% to about 30%, including from about 1% to about 20%, including from about 1% to about 15%, and which also includes from about 1.5% to about 5%, by weight of the nutritional emulsion. In a specific embodiment, a nutritional emulsion includes fats at about 1.6%, by weight of the nutritional emulsion.

industrial

<img file="MX347477B_D0016.tif" />

It should be recognized by one of ordinary skill in the art that in some embodiments, such as a substantially clear liquid beverage, the nutritional product will be substantially free of fat.

When used in solid nutritional products, fats may be present in an amount from about 1% to about 35%, including from about 1% to about 20%, including from about 1% to about 15%, and also including from about 5.0% to about 10%, by weight of the solid nutritional product. In a specific embodiment, a spray dried nutritional powder includes the fats in an amount of about 7.5%, by weight of the spray dried nutritional powder.

Convenient sources of fat for use herein include any fat or fat source that is convenient for use in an oral nutritional product and is compatible with the essential elements and characteristics of such products.

Non-limiting examples of suitable fats or sources thereof for use in the nutritional products described herein include coconut oil, fractionated coconut oil, soybean oil, corn oil, olive oil, ₅₀ ...---—-----------—--—

IMPI safflower oil, safflower oil with a11 MCT oil (medium chain trigi icers), sunflower oil, high oleic sunflower oil, palm and palm kernel oil, palm olein, Canola, oils of marine origin, cottonseed oils, and combinations thereof.

Other optional ingredients

Nutritional products, including infant formulas, that have the pea protein hydrolyzate of the present disclosure, may additionally comprise other optional components that can modify the physical, chemical, aesthetic or processing characteristics of the products or that serve as components. pharmaceutical or additional nutritional supplements when used in the target population. Many of such optional ingredients are known or otherwise suitable for use in medical food products or other nutritional products or pharmaceutical dosage forms and may also be used in the compositions herein, provided that such optional ingredients are safe for oral administration and compatible with essential ingredients and other ingredients in the selected product form.

Non-limiting examples of such optional ingredients include preservatives, antioxidants, emulsifying agents, buffers, fructo-oligosaccharides, galacto-oligosaccharides, milk oligosaccharides.

IMFIi ^

INSTITUTO MEXICANO huW '^ and pharmaceutical active prebiotics additional nutrients as described herein, colorants, flavors, thickening and stabilizing agents, emulsifying agents, lubricants, etcetera.

Examples of suitable HMOs that can be included in the compositions herein include lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose I, lacto-N-fucopentaose II, lacto-N-fucopentaose III. , lacto-N-fucopentaose V, lacto-N-hexaose, para-lacto-N-hexaose, lacto-Nneohexaose, para-lacto-N-neohexaose, monofucosyl-lacto-Nhexaose II, isomeric fucosylated lacto-N-hexaose (1), monofucosyl-lacto-N-hexaose, isomeric fucosylated lacto-N-hexaose (3), Isomeric fucosylated lacto-N-hexaose (2), difucosylpara-lacto-N-neohexaose, difucosyl-para-lacto-N-hexaose, difucosyl-lacto-N-hexaose, lacto-N-neoocataose, para-lacto-Noctanose, iso -lacto-N-octaose, lacto-N-octaose, monofucosylI act-neo ocata os a, monofucosyl-lacto-N-ocataose, difucosyl-lactoN-octaose I, difucosyl-lacto-N-octaose II, difucosyl-lacto-Nneoocataose II, difucosyl-lacto-N-neoocataose I, lacto-N-decaose, trifucosyl-lacto-N-neooctaose, trifucosyl-lacto-N-octaose, trifucosyliso-lacto-N-octaose, and combinations thereof. These HMOs are more fully described in US Patent Application Number 2009/0098240, which is incorporated herein by reference in its entirety. Other examples x -II. INSTITUTO MJ.XICANC -r-'í; A convenient HMO compositions of the present disclosure include lacto-N-difukohexaose II, sialyl (2-3) lactose, sialyl (2-6) lactose, sia I i I -1 a ct oN tetraose a, sialyl -lacto-N-tetraose b, sialyl-lacto-N-tetraose c, sialyl-fucosyl-lacto-N-tetraose I, sialyl-fucosyl-lacto-N-tetraose II, and disialyl-lacto-N-tetraose.

The compositions may further comprise a sweetening agent, preferably including at least one sugar alcohol such as maltitol, erythritol, sorbitol, xylitol, mannitol, isomalt, and lactitol, and preferably also include at least one artificial or high potency sweetener such as acesulfame K, aspartame, sucralose, saccharin, stevia, and tagatose. These sweeteners, especially as a combination of a sugar alcohol and an artificial sweetener, are especially useful in formulating the liquid beverage embodiments of the present disclosure that have a desirable flavor profile. These sweetener combinations are especially effective in masking the undesirable flavors occasionally associated with the addition of plant proteins to a liquid beverage. Optional concentrations of the sugar alcohol in the nutritional product can range from at least about 0.01%, including from 0.1% to about 10%, and also including from about 1% to about 6%, by weight of the nutritional product. Optional concentrations of the artificial sweetener can range from approximately 0.01%, which includes appro

<img file="MX347477B_D0017.tif" />

to about 5%, which also includes about

0.1% to about 1.0%, by weight of the nutritional product.

A flow agent or the anti-caking agent can be included in nutritional products as nutritional powders as described herein to retard agglomeration or caking of the powder over time and to make a powder modality flow easily from its container. Any flow or anti-caking agent known or otherwise suitable for use in a nutritional product or powder form are suitable for use herein, non-limiting examples of which include tricalcium phosphate, silicate, and combinations of the same. The concentration of the flow agent or anti-caking agent in the nutritional product varies depending on the product form, the other ingredients selected, the desired flow characteristics, etc., but very commonly they range from about 0.1% to about 4%, which includes from about 0.5% to about 2%, by weight of the nutritional product.

A stabilizer can also be included in nutritional products. Any stabilizer that is known or otherwise convenient for use in a nutritional product is also suitable for use herein, some of its non-limiting examples include gums such as xanthan gum.

The stabilizer ntsrrruTO msxican can about 0.1% to about 50%, including from about 0.5% to about 3%, including from about 0.7% to about 1.5%, by weight of the nutritional product.

The compositions may further comprise any of a variety of other vitamins or related nutrients, non-limiting examples of which include vitamin A, vitamin D, vitamin E, vitamin K, thiamine, riboflavin, pyridoxine, vitamin B12, carotenoids (eg, beta-carotene, zeaxanthin , lutein, lycopene), niacin, folic acid, pantothenic acid, biotin, vitamin C, choline, inositol, salts and derivatives thereof, and combinations thereof.

The compositions may further comprise any of a variety of other additional minerals, non-limiting examples of which include calcium, phosphorus, magnesium, iron, zinc, manganese, copper, sodium, potassium, molybdenum, chromium, chloride, and combinations thereof.

Manufacturing methods

The nutritional products of the present disclosure can be prepared by known manufacturing technique or otherwise effective in preparing the selected form of the solid or liquid product. Many such techniques are known for any given product form such as nutritional liquids or powders or nutritional bars and can be applied

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DE LA PROFIEDAO ίΤΗ easily by one skilled in the art to the nutritional ffCHT ^ bsicle ^ es described herein. ............ -

The nutritional products of the present disclosure, therefore, can be prepared by any of a variety of known or otherwise effective formulation or manufacturing methods. In a convenient manufacturing process, for example, at least three separate suspensions are prepared, including a protein-in-fat (PIF) suspension, a carbohydrate-mineral (CHOMIN) suspension English), a protein suspension in water (PIW). The PIF suspension is formed by heating and mixing the oil (for example, rapeseed oil, corn oil, etc.) and then adding an emulsifier (for example, lecithin), fat-soluble vitamins, and a portion of the protein. total (eg, milk protein concentrate, etc.) with continuous heat and stirring. The CHO-MIN mixture is formed by adding to the water the stirred and heated minerals (for example, potassium citrate, dipotassium phosphate, sodium citrate, etc.), trace elements and ultra-trace elements (TM / UTM premix), thickening agents or suspending agents (eg avicel, gellan, carrageenan). The resulting CHO-MIN suspension is held for 10 minutes with continuous heat and stirring before adding additional minerals (e.g., potassium chloride, magnesium carbonate, potassium iodide, etc.), and / or carbohydrates (e.g. sucrose, fructo-oligosaccharide

<img file="MX347477B_D0018.tif" />

etc). The PIW suspension is then formed by mixing the remaining protein, if any, with heat and agitation.

The resulting suspensions are then mixed together with heat and agitation and the pH is adjusted to 6.6-7.0, after which the composition is subjected to high temperature short-term processing (HTST) during which the The composition is heat treated, emulsified and homogenized, and then allowed to cool. The water-soluble vitamins and ascorbic acid are added, the pH is adjusted to the desired range if necessary, the flavors are added, and the water is added to achieve the desired level of total solid. The composition is then packaged aseptically to form an aseptically packaged nutritional emulsion. This emulsion can then be further diluted, heat-treated, and packaged to form the ready-to-drink liquid or concentrated liquid, or it can be heat-treated and further processed and packaged as a reconstitutable powder, e.g. dried. by spraying, dry mixing, and agglutinating.

When a substantially clear liquid beverage is desired, the manufacturing process described above is modified such that only a suspension of CHOMIN and a suspension of PIW are mixed to form the beverage.

IMPIOS Mexican INSTITUTE 55

3t LA ΓΚΟΗΙΩΑΓ nutritional liquid. industrial

Other forms of product such as bars — irnutritionaloo oo. they can be manufactured, for example, by using cold extrusion technology as is known and commonly described in the bar-making art. To prepare such products, commonly all of the powdered components are dry mixed together, which commonly includes any protein, vitamin premixes, certain carbohydrates, and the like. The fat soluble components are then mixed together and mixed with any powdered premix. Finally any liquid component is then mixed into the composition, which forms a plastic-like composition or paste. The resulting elastic mass can then be molded, without other physical or chemical changes occurring, by cold forming or extrusion, where the elastic mass is forced at relatively low pressure through a die, which confers the desired shape. The resulting extrudate is then cut into an appropriate position to provide products of the desired weight. If desired, the solid product is then coated, to enhance palatability, and packaged for distribution.

Other convenient methods for making nutritional products are described, for example, in US Patent Numbers 6,365,218 (Borschel, et al.), 6,589,576 (Borschel, et al.), 6,306,908 (Carlson, et al), and in US Patent No.

INSTITUTO MEXICAN!)

US Patent Application 200301 18703 al.), The disclosures of which are incorporated herein by reference to the extent consistent with the present.

Methods of use

Nutritional products that include pea protein hydrolysates as the sole protein source are useful as a source of hypoallergenic nutrition and / or as a source of semi-elemental nutrition for premature babies, infants, toddlers, children, adults, and the elderly who are intolerant and / or allergic to cow's milk proteins, soy proteins, and / or who suffer from other general food allergies. In addition, nutritional products may be particularly suitable for use by individuals suffering from malabsorption, maldigestion, and other gastrointestinal conditions.

In one embodiment, nutritional products may be useful to provide a source of nutrition when using hydrolyzed proteins and to have better organoleptic properties, as in sports drinks.

Nutritional products can be administered orally as needed to provide the desired level of nutrition, most commonly in the form of one, two, three, four, five, six, seven, eight, or more servings daily. Serving sizes can vary widely and can range from about 1 to about 500 ml, which includes from about 5 to about 250 ml, which includes from

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about 10 to about 240 mi. "Ήχ * ™

Examples ____________

The following examples illustrate the specific modalities and / or characteristics of the pea protein hydrolysates and / or nutritional products of the present disclosure. The examples are given for the purpose of illustration only and should not be construed as limitations of the present description, many variations thereof are possible without departing from the spirit and scope of the description. All amounts exemplified are percentages by weight based on the total weight of the composition, unless otherwise specified.

The exemplified compositions are nutritional products prepared according to manufacturing methods well known in the nutrition industry for preparing nutritional emulsions, other liquid nutritional drinks, nutritional powders, and / or nutritional bars. Example 1

In this example, 10 samples of the filtered (F) and (N) unfiltered pea protein hydrolysates prepared according to the process as described herein are evaluated and compared with a sample of the pea protein isolate. intact (ISO) to determine various physical characteristics. Filtering (F) indicates that the sample is subjected to the second stage of filtration described

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OF THE PROPERTY above. All samples are subjected to the pWfTWa erapra · filtration described above. The ctiiIiuI (Intact) sample does not undergo any hydrolysis process. Specifically, samples are evaluated for degree of hydrolysis, molecular weight distribution, isoflavone content, and phytate content using standard analytical techniques. The results of the evaluations are shown in the following table.

<td>Samples</td><td>Intact</td><td>AN</td><td>AF</td><td>BN</td><td>BF</td><td>CN</td><td>CF</td><td>DN</td><td>DF</td><td>IN</td><td>EF</td>

<td colspan="12">Quality</td>

<td>% of Nitrogen</td><td> 12.90</td><td> 13.30</td><td> 13.60</td><td> 13.20</td><td> 14.00</td><td> 12.90</td><td> 13.60</td><td> 13.10</td><td> 14.00</td><td> 12.90</td><td> 13.90</td>

<td>%from Protein</td><td> 80.63</td><td> 83.13</td><td> 85</td><td> 82.5</td><td> 87.5</td><td> 80.63</td><td> 85</td><td> 81.88</td><td> 87.5</td><td> 80.63</td><td> 86.88</td>

<td colspan="12">% Nitrogen X 6.25 of the approximate NA</td>

<td>Prot. Of %From aa B + F</td><td> 82.05</td><td> 81.34</td><td> 88.83</td><td> 81.12</td><td> 88.64</td><td> 80.20</td><td> 83.03</td><td> 82.70</td><td> 91.27</td><td> 83.65</td><td> 94.36</td>

<td>Amino N</td><td> 1.12</td><td> 1.68</td><td> 2.05</td><td> 2.2</td><td> 2.39</td><td> 2.88</td><td> 2.98</td><td> 2.95</td><td> 3.31</td><td> 3.41</td><td> 3.97</td>

<td>DH (% from AN / TN)</td><td> 8.68</td><td> 12.63</td><td> 15.07</td><td> 16.67</td><td> 17.07</td><td> 22.17</td><td> 21.91</td><td> 22.75</td><td> 23.64</td><td> 26.43</td><td> 28.56</td>

<td>Adjusted DH</td><td> 0.00</td><td> 3.95</td><td> 6.39</td><td> 7.99</td><td> 8.39</td><td> 13.49</td><td> 13.23</td><td> 14.07</td><td> 14.96</td><td> 17.75</td><td> 19.88</td>

IMPIOS Mexican institute) rw ia 99 wrrun · .- .. '—V. ·'. Z 1

<td></td><td>Samples</td><td>Intact</td><td>AN</td><td>AF</td><td>BN</td><td>BF</td><td>CN</td><td>CF</td><td>DN</td><td>OF THE PR df^ene</td><td>AiUAHL /? P! AGE USTg ^</td><td></td><td></td>

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> 5</td><td>Mg per 100 ml in 5 mg protein / ml</td><td> 620</td><td> 601</td><td> 588</td><td> 606</td><td> 571</td><td> 620</td><td> 588</td><td> 610</td><td> 571</td><td> 620</td><td> 576</td><td></td>

<td> 10</td><td>Eq. in g = 500 ml in 2% of Prot.</td><td> 12.4</td><td> 12.02</td><td> 11.75</td><td> 12.12</td><td> 11.42</td><td> 12.4</td><td> 11.76</td><td> 12.2</td><td> 11.42</td><td> 12.4</td><td> 11.52</td><td></td>

<td></td><td colspan="3">MW range by HPLC</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td>&gt; 3 KD</td><td> 97.00 %</td><td> 37.50 %</td><td> 27.20 %</td><td> 10.40 %</td><td> 4.80 %</td><td> 6.70 %</td><td> 1.70 %</td><td> 6.90 %</td><td> 2.60 %</td><td> 5.80 %</td><td> 1.60 %</td><td></td>

<td></td><td>&gt; 5 KD</td><td> 93.30 %</td><td> 26.30 %</td><td> 16.50 %</td><td> 4.10 %</td><td> 1.00 %</td><td> 2.70 %</td><td> 0.40 %</td><td> 2.50 %</td><td> 0.60 %</td><td> 2.00 %</td><td> 0.40 %</td><td></td>

<td> 15</td><td>3-5 KD</td><td> 3.70 %</td><td> 11.20 %</td><td> 10.70 %</td><td> 6.30 %</td><td> 3.80 %</td><td> 4.00 %</td><td> 1.30 %</td><td> 4.40 %</td><td> 2.00 %</td><td> 3.80 %</td><td> 1.20 %</td><td></td>

<td></td><td>1-3 KD</td><td> 1.00 %</td><td> 28.80 %</td><td> 34.50 %</td><td> 31.30 %</td><td> 33.10 %</td><td> 18.10 %</td><td> 17.80 %</td><td> 22.90 %</td><td> 24.60 %</td><td> 18.40 %</td><td> 19.20 %</td><td></td>

<td> 20</td><td>0.5-1 KD</td><td> 0.40 %</td><td> 20.30 %</td><td> 23.60 %</td><td> 30.40 %</td><td> 33.20 %</td><td> 29.30 %</td><td> 31.80 %</td><td> 29.40 %</td><td> 31.60 %</td><td> 28.10 %</td><td> 30.30 %</td><td></td>

<td></td><td>&lt;0.5 KD</td><td> 1.60 %</td><td> 13.40 %</td><td> 14.70 %</td><td> 27.90 %</td><td> 28.90 %</td><td> 45.90 %</td><td> 48.70 %</td><td> 40.80 %</td><td> 41.20 %</td><td> 47.70 %</td><td> 48.90 %</td><td></td>

<td></td><td colspan="4">Phytoestrogen content</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td> 25</td><td>Daldzein in mg / kg</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td></td>

<td colspan="12"></td><td rowspan="6"></td>

<td rowspan="2">Samples</td><td rowspan="2">Intact</td><td rowspan="2">AN</td><td rowspan="2">AF</td><td rowspan="2">BN</td><td rowspan="2">BF</td><td rowspan="2">CN</td><td rowspan="2">CF</td><td>DN</td><td>GIVE THE PRí DF¹™</td><td>Atv-níNL / * PIETY ¹</td><td></td>

<td></td><td></td><td></td><td></td>

<td>Genistein in mg / kg</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td><td> &lt;2</td>

<td>Total fos. in g / kg</td><td> 8.3</td><td> 8.55</td><td> 6.41</td><td> 8.3</td><td> 4.58</td><td> 8.27</td><td> 5.73</td><td> 8.27</td><td> 5.05</td><td> 8.21</td><td> 4.89</td>

<td>Phytic acid in g / kg</td><td> 20.6</td><td> 21.2</td><td> 15.9</td><td> 20.6</td><td> 11.4</td><td> 20.6</td><td> 14.2</td><td> 20.6</td><td> 12.5</td><td> 20.4</td><td> 12.2</td>

Example 2

In this example, the hypoallergenic potential of the pea protein hydrolysates analyzed in Example 1 is evaluated and compared to the intact pea protein isolate (ISO) sample from Example 1 using hyperimmunization. of rabbits, in which rabbits are given the multiple immunizations for 35 days as will be described later. The immunogenicity of pea protein hydrolysates is then tested by ELISA (enzyme-linked immunosorbent assay), which measures the response of rabbit antigen-specific IgG to the intact pea protein / pea protein hydrolyzate immunogen.

The rabbits used in this example are put on a diet free of cow's milk, soy, and pea proteins for fourteen days prior to the first immunization. On day 0, the baseline antibody status of each rabbit was

IMPI evaluates with the blood sample of the corref to obtain the baseline, each rabbit is immunized with 5.0 mg of the equivalent amino acid content of pea protein included in 4.5 ml of antigen emulsified in complete Freund's adjuvant (CFA, for its acronym in English). Immunizations are given as follows: 20 x 0.1 ml per site / flank shaved intradermally, and 0.25 ml / popliteal area of ​​the hind paw lymph node. On day 21, the second immunization is as follows: 0.50 ml / site X 2 sites / hind paw, intramuscular; and, 0.25 ml / site X 4 sites subscapularly. For each immunization, a constant dose of 5 mg amino acid equivalent of antigen is administered. The exsanguination of rabbits is on day 35 of the study.

A direct ELISA is used to quantify the antibody responses for each rabbit after hyperimmunization. Analysis optimization is achieved by using block titrations to determine antigen coating concentration and conjugate dilution. After optimization of assays, pea protein-specific rabbit antibodies are quantitated in serum samples from day 0 and day 35. Additionally, an immune response index (IRI units) is determined by dividing the normalized antibody titer on day 35 by the normalized baseline titer (day 0) on day 0 for each antiserum. The immunization groups contain 5-10 animals and the results for each animal are averaged. The

IMPI Mexican institute comparisons of IRI es pecff antigen results predict hypnaiArgenic clinical performance. The results are provided in the following table:

<td colspan="12">Immune response index after hyperimmunization (Normalized titer on day 35 / Normalized titer on day 0)</td>

<td></td><td>Intact</td><td>AN</td><td>AF</td><td>BN</td><td>BF</td><td>CN</td><td>CF</td><td>DN</td><td>DF</td><td>IN</td><td>EF</td>

<td>IRI Units</td><td> 7155</td><td> 9002</td><td> 7558</td><td> 330.8</td><td> 167.8</td><td> 167.2</td><td> 10.73</td><td> 921.4</td><td> 932.8</td><td> 1027</td><td> 144.5</td>

<td>Units Logde IRI</td><td> 3.8546</td><td> 3.9543</td><td> 3.8784</td><td> 2.5196</td><td> 2.2245</td><td> 2.2232</td><td> 1.0307</td><td> 2.9644</td><td> 2.9698</td><td> 3.0115</td><td> 2.16</td>

As shown in the table, pea protein hydrolysates show a wide range of antibody response. Low reactivity may indicate that pea protein hydrolysates still have epitopes that are recognized as antigens, but not immunogenic by rabbits. On the other hand, the pea protein hydrolysates BN, FB, NC, and CF showed significantly reduced immunogenicity after hyperimmunization relative to the other samples. The reduced immunogenicity of the pea protein hydrolyzate samples indicates that conserved pea antigens do not have the ability to stimulate an immune response.

Example 3

In this example, the hypoallergenic potential of

ΙΜΡΙ® ^

......,,, ... INSTITUTO MEXICANO pea protein hydrolysates analyzed are evaluated and compared with the intact pea protein isolate (ISO) sample from Example 1 using the antigen content, measured with the inhibition ELISA. In this analysis, the intact pea protein is coated on the ELISA plates. Incubation tubes containing the pea protein samples or solutions at standard concentrations plus anti-pea protein antibodies are prepared separately. After an incubation period, aliquots of the sample (or standard) plus the antibody solutions are added to the coated plates. After incubation and washing, an anti-rabbit IgG antibody conjugated to the enzyme is added. Plates are incubated, washed, and an enzyme substrate is added. A staining reaction occurs, which is inversely proportional to the concentration of pea antigen in the sample, which is quantitated according to a standard curve. The results of this test for the 10 samples are shown in the following table:

<td colspan="12">immune response rate after hyperimmunization (Standardized title on day 35 / Standardized title on day 0)</td>

<td></td><td>Intact</td><td>AN</td><td>AF</td><td>BN</td><td>BF</td><td>CN</td><td>CF</td><td>DN</td><td>DF</td><td>IN</td><td>EF</td>

<td>Antigen □ g / g protein</td><td> 1,000,000</td><td> 493009</td><td> 431210</td><td> 55413</td><td> 36007</td><td> 15013</td><td> 6417</td><td> 18504</td><td> 14900</td><td> 14215</td><td> 8644 _</td>

Example 4

<img file="MX347477B_D0019.tif" />

IMPIg

In this example, the sensory evaluation ^^^^ g ^

INDUSTRIAL two area research sessions to characterize the taste of the pea protein hydrolyzate samples from Example 1 within the retort both in water and on an Alimentum® matrix (Abbott Laboratories, Columbus, Ohio). The Alimentum® matrix contains carbohydrates, fats, vitamins, and minerals to simulate the biochemistry of a hypoallergenic formula. It is used to determine the mutual organoleptic effects on the hydrolyzate and the finished formula relative to each other within the product matrix after retort treatment. The samples are compared with the current hydrolyzate (casein hydrolyzate) used in Alimentum® and the intact pea protein isolate (ISO) from example 1. In particular, it is known that hydrolyzed proteins are generally bitter, sour, salty, and / or fruity flavor. Therefore, it is difficult to incorporate this functional ingredient into nutritional formulations because it drastically damages the taste of the product.

Samples are analyzed for the intensity of various flavors, including for example: bitter, salty, sour, soupy, wet wool, sour sour, grassy / soybean, starchy, woody / sawdust, green pea / fruity / dry, dryness, powdery , oily, sulphurous / cabbage, burnt cheese, and gritty. Specifically, once the samples were prepared, four trained flavor descriptors tasted each

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<img file="MX347477B_D0020.tif" />

_____, Say LA WOilEDAP „sample. After tasting, each member determined taste qualities of the sample by using di?

explained in the following table and the results are then averaged.

<td>Counting</td><td>Description</td>

<td> 1/2</td><td>Very light</td>

<td> 1</td><td>Light</td>

<td> 1 1/2</td><td>Light to moderate</td>

<td> 2</td><td>Moderate</td>

<td> 2 1/2</td><td>Moderate to intense</td>

<td> 3</td><td>Intense</td>

Hydrolyzed in water (2% solution)

<td>Basic and aromatic flavors</td><td>AF</td><td>BF</td><td>CF</td><td>DF</td><td>EF</td><td>Hydrolyzed Alimentum® current</td>

<td>Bitter</td><td> 1</td><td> 1 7₂</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td>

<td>Salt</td><td> 1</td><td> 1</td><td> 72</td><td> 72</td><td> 1</td><td></td>

<td>Sour</td><td> 7₂</td><td> 72</td><td> 72</td><td> 72</td><td> 1</td><td> 1</td>

<td>Soupy</td><td> 1</td><td> 1</td><td>1 7a</td><td>1 7a</td><td> 2</td><td> 2 ½</td>

<td>Wool damp</td><td> 1 7₂</td><td> 1 72</td><td> 1 7₂</td><td> 1 72</td><td> 2</td><td> 2 7₂</td>

<td></td><td></td><td></td><td></td><td></td><td colspan="2">IMPIíg ^</td><td></td>

<td>Flavors basic</td><td rowspan="2">AF</td><td rowspan="2">BF</td><td rowspan="2">CF</td><td></td><td>IUIU MtAILA / CH LA PROPERTY / INDUSTRI</td><td>r Hydrolyzed</td><td></td>

<td>Y aromatic</td><td>DF</td><td>tF</td><td>^ ΑΙΙΙΗβΗΤΙΠΗ * current</td><td></td>

<td>Sour sour</td><td></td><td></td><td></td><td></td><td></td><td> 1</td><td></td>

<td>Appearance:</td><td>Translucent pale yellow</td><td>Yellow pale translucent</td><td>Yellow pale translucent</td><td>Yellow pale translucent</td><td>Translucent pale yellow</td><td>Yellow translucent gold</td><td></td>

Hydrolysates not filtered in water (2% solution)

<td>Basic and aromatic flavors</td><td>AN</td><td>BN</td><td>CN</td><td>DN</td><td>IN</td><td>ISO</td>

<td>Bitter</td><td> 1</td><td> 1</td><td> 1 72</td><td> 1</td><td> 1</td><td> 7</td>

<td>Salt</td><td></td><td></td><td></td><td></td><td> 1</td><td> 7</td>

<td>Sour</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td>

<td>Soupy</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1 7</td><td></td>

<td>Sour sour</td><td></td><td></td><td></td><td></td><td> 1</td><td></td>

<td>Fatty / Soy bean</td><td> 2</td><td> 2</td><td> 1</td><td> 1 7</td><td> 1 7</td><td></td>

<td>Starchy</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td>

WICKED

INSTITUTO MEXICANO Vt «jl ________ * —i. T rTi&gt;?

<td>Basic and aromatic flavors</td><td>AN</td><td>BN</td><td>CN</td><td>DN</td><td>I -6N--</td><td>NDÜSTNAL --- ISO ---</td>

<td>Woody / Sawdust</td><td></td><td></td><td> 2</td><td> 1</td><td> 1</td><td></td>

<td>Pea / Afrijolado / Dry</td><td></td><td></td><td></td><td></td><td></td><td> 1 72</td>

<td>Dry</td><td> 2</td><td> 2</td><td> 2</td><td> 2</td><td> 2</td><td> 2</td>

<td>Dusty mouthfeel</td><td> 1 %</td><td> 1 %</td><td>1 and₂</td><td>1 V₂</td><td> 1 7₂</td><td> 1 7₂</td>

<td>Appearance:</td><td>Cloudy light brownish yellow</td><td>Cloudy light brownish yellow</td><td>Cloudy light brownish yellow</td><td>Cloudy light brownish yellow</td><td>Cloudy light brownish yellow</td><td>Dull light brown yellow</td>

Hydrolysates filtered in the Alimentum® matrix

<td>Basic and aromatic flavors</td><td>AF</td><td>BF</td><td>CF</td><td>DF</td><td>EF</td><td>Hydrolyzed Alimentum® current</td>

<td>Bitter</td><td> 72</td><td> 72</td><td> 72</td><td> 72</td><td> 1</td><td> 1</td>

<td>Salt</td><td> 72</td><td> 72</td><td> 72</td><td> 72</td><td> 72</td><td> 1</td>

<td></td><td></td><td></td><td></td><td></td><td colspan="2">IMPIOUS MEXICAN INSTITUTE</td><td rowspan="2"></td>

<td>Flavors basic</td><td></td><td></td><td></td><td></td><td>OF THE H INI</td><td>HjSTRIAL PIETY Hydrolyzed</td>

<td>Y aromatic</td><td>AF</td><td>BF</td><td>CF</td><td>DF</td><td>EF</td><td>Alimentum® current</td><td></td>

<td>Sour</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td></td>

<td>Sweet</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 7₂</td><td> 1 7</td><td></td>

<td>Soupy</td><td>2 turkey</td><td> 1 %</td><td> 1 ¹/₂</td><td>2 turkey</td><td>2 turkey</td><td> 1 ¹/₂</td><td></td>

<td>Sour acid</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1 ¹/₂</td><td></td>

<td>Dry</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td></td>

<td>Oily mouthfeel</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td></td>

<td>Sulphorous / Cabbage</td><td></td><td></td><td></td><td></td><td></td><td> 2</td><td></td>

<td>cheese Burned</td><td></td><td></td><td></td><td></td><td></td><td> 1 7₂</td><td></td>

<td>Wool damp</td><td></td><td></td><td></td><td></td><td></td><td> 7₂</td><td></td>

<td>Appearance:</td><td>Dull pale gray with yellow</td><td>Dull pale gray</td><td>Dull pale gray</td><td>Dull pale gray</td><td>Dull pale gray</td><td>Dull pale gray</td><td></td>

WICKED

INSTITUTO MEXICANA ώ '·&gt;' Δ

Unfiltered hydrolysates in the Alimentum® matrix »™ ^

<td rowspan="2">Basic and aromatic flavors</td><td rowspan="2">AN *</td><td rowspan="2">BN *</td><td rowspan="2">CN *</td><td rowspan="2">DN *</td><td colspan="2"></td><td></td>

<td>IN*</td><td>ISO *</td><td></td>

<td>Bitter</td><td> 1</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 7₂</td><td> 1 ¹/₂</td><td> 72</td><td></td>

<td>Salt</td><td>¹/₂</td><td>Y₂</td><td>¹/₂</td><td>¹/₂</td><td> 7₂</td><td> 72</td><td></td>

<td>Sour</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td></td>

<td>Sweet</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 7₂</td><td> 1 7₂</td><td></td>

<td>Soupy</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td></td><td></td>

<td>Sour acid</td><td> 1 ¹/₂</td><td> 2</td><td> 2</td><td> 2</td><td> 2</td><td></td><td></td>

<td>Woody /Sawdust</td><td> 1 ¹/₂</td><td>1 and₂</td><td>1 and₂</td><td> 1 7</td><td> 1 7₂</td><td></td><td></td>

<td>Dry</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td> 1</td><td></td>

<td>Sensation</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td>n buccal</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 ¹/₂</td><td> 1 7₂</td><td></td><td></td>

<td>dusty</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td>Sensation</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td>nb ucal</td><td></td><td></td><td></td><td></td><td></td><td> 2</td><td></td>

<td>sandy</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td>Pea/</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td>afrijolado /</td><td></td><td></td><td></td><td></td><td></td><td> 2</td><td></td>

I Μ. ΡI

INSTITUTE mJ¡Can &lt;,

OF THE RR.O '(AGE ^. &lt;' 3

<td>Flavors basic</td><td rowspan="2">AN *</td><td rowspan="2">BN *</td><td rowspan="2">CN *</td><td rowspan="2">DN *</td><td>INSTITUTE MEX OF THE INDU RR.</td><td>ITRIAL IEDAD</td><td></td>

<td>and aromatic</td><td>Γ t rr—</td><td>ISO * ^{, j}</td><td></td>

<td>Appearance:</td><td>Dull light brown with gray</td><td>Dull light brown with gray</td><td>Dull light brown with gray</td><td>Dull light brown with gray</td><td>Dull light brown with gray</td><td>Dull light brown with gray</td><td></td>

Examples 5-34

Examples 5-34 illustrate various infant formula nutritional powders of the present disclosure that include pea protein hydrolyzate as at least one source of protein, and in some cases the only source of protein, the ingredients of which are listed in the following table. The pea protein hydrolyzate has a degree of hydrolysis (adjusted) of approximately 7.0% to less than 19.0%. These products are prepared through various methods in separate batches, reconstituted with water prior to use at the desired concentrations of the target ingredient. The modalities explained below that include pea protein hydrolyzate as the only protein source are hypoallergenic modalities.

Examples 5-9

IMPI

<td>Ingredient</td><td colspan="5">Amounts per 227 ^ jÍYES ^ Xl</td>

<td rowspan="2"></td><td rowspan="2">Example 5</td><td rowspan="2">Example 6</td><td></td><td></td><td>ι_ΕϊβτηηΙο</td>

<td> 7</td><td> 8</td><td> 9</td>

<td>Corn syrup</td><td>9091.6 kg</td><td>9091.6 kg</td><td>8925.1 kg</td><td>9091.6 kg</td><td>8758.7 kg</td>

<td>Protein hydrolyzate pea</td><td>3329.4 kg</td><td>3162.9 kg</td><td>3495.9 kg</td><td>2996.5 kg</td><td>3662.3 kg</td>

<td>High oleic safflower oil</td><td>2608.1 kg</td><td>2608.1 kg</td><td>2608.1 kg</td><td>2608.1 kg</td><td>2608.1 kg</td>

<td>Sugar (sucrose)</td><td>2328.6 kg</td><td>2495.1 kg</td><td>2328.6 kg</td><td>2661.5 kg</td><td>2328.6 kg</td>

<td>Soy oil</td><td>1908.3 kg</td><td>1908.3 kg</td><td>1908.3 kg</td><td>1908.3 kg</td><td>1908.3 kg</td>

<td>Coconut oil</td><td>1844.7 kg</td><td>1844.7 kg</td><td>1844.7 kg</td><td>1844.7 kg</td><td>1844.7 kg</td>

<td>5% potassium hydroxide</td><td>600 kg</td><td>600 kg</td><td>600 kg</td><td>600 kg</td><td>600 kg</td>

<td>Potassium citrate # 1, # 2</td><td>279.8 kg</td><td>279.8 kg</td><td>279.8 kg</td><td>279.8 kg</td><td>279.8 kg</td>

<td>Tribasic calcium phosphate</td><td>252.5 kg</td><td>252.5 kg</td><td>252.5 kg</td><td>252.5 kg</td><td>252.5 kg</td>

<td>Dibasic calcium phosphate</td><td>147.6 kg</td><td>147.6 kg</td><td>147.6 kg</td><td>147.6 kg</td><td>147.6 kg</td>

<td>Sodium chloride</td><td>76.2 kg</td><td>76.2 kg</td><td>76.2 kg</td><td>76.2 kg</td><td>76.2 kg</td>

<td>Magnesium chloride</td><td>58.8 kg</td><td>58.8 kg</td><td>58.8 kg</td><td>58.8 kg</td><td>58.8 kg</td>

<td>L-methionine</td><td>36.2 kg</td><td>36.2 kg</td><td>36.2 kg</td><td>36.2 kg</td><td>36.2 kg</td>

<td>Ascorbic acid</td><td>36.1 kg</td><td>36.1 kg</td><td>36.1 kg</td><td>36.1 kg</td><td>36.1 kg</td>

<td>Vitamin / Mineral / Taurine Premix</td><td>32.5 kg</td><td>32.5 kg</td><td>32.5 kg</td><td>32.5 kg</td><td>32.5 kg</td>

<td>Choline Chloride</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td>

IMPI®

INSTITUTO MEXI- ^ NC

<td>Ingredient</td><td colspan="5">Amounts per 22727.31? ^^</td>

<td></td><td>Example 5</td><td>Example 6</td><td>Copy 7</td><td> 8</td><td> 9</td>

<td>Vitamin A premix, D3, E, &amp; K1</td><td>8.7 kg</td><td>8.7 kg</td><td>8.7 kg</td><td>8.7 kg</td><td>8.7 kg</td>

<td>Ascorbyl Palmitate</td><td>8.1 kg</td><td>8.1 kg</td><td>8.1 kg</td><td>8.1 kg</td><td>8.1 kg</td>

<td>Ferrous sulfate</td><td>6.9 kg</td><td>6.9 kg</td><td>6.9 kg</td><td>6.9 kg</td><td>6.9 kg</td>

<td>Potassium chloride</td><td>4.3 kg</td><td>4.3 kg</td><td>4.3 kg</td><td>4.3 kg</td><td>4.3 kg</td>

<td>Tocopherols mixer</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td>

<td>L-carnitine</td><td>2.5 kg</td><td>2.5 kg</td><td>2.5 kg</td><td>2.5 kg</td><td>2.5 kg</td>

<td>Beta-carotene in oil vegetable</td><td>28.3 g</td><td>28.3 g</td><td>28.3 g</td><td>28.3 g</td><td>28.3 g</td>

<td>Potassium iodide</td><td>22.9 g</td><td>22.9 g</td><td>22.9 g</td><td>22.9 g</td><td>22.9 g</td>

Examples 10-14

<td>Ingredient</td><td colspan="5">Amounts per 22727.3 kg</td>

<td></td><td>Example 10</td><td>Example eleven</td><td>Example 12</td><td>Example 13</td><td>Example 14</td>

<td>Corn syrup</td><td>9091.6 kg</td><td>9091.6 kg</td><td>9091.6 kg</td><td>9091.6 kg</td><td>9091.6 kg</td>

<td>Pea protein hydrolyzate</td><td>2996.5 kg</td><td>2663.5 kg</td><td>2330.6 kg</td><td>1997.6 kg</td><td>1664.7 kg</td>

<td>Soy protein isolate</td><td>332.9 kg</td><td>665.9 kg</td><td>998.8 kg</td><td>1331.8 kg</td><td>1664.7 kg</td>

<td>High oleic safflower oil</td><td>2608.1 kg</td><td>2608.1 kg</td><td>2608.1 kg</td><td>2608.1 kg</td><td>2608.1 kg</td>

IMPI -------------------------- iwrrmrrn Mexican Amounts per each

<img file="MX347477B_D0021.tif" />

Ingredient

<td rowspan="2"></td><td rowspan="2">Example 10</td><td rowspan="2">Example eleven</td><td>Example.</td><td>Eiemnlo</td><td>Eiemolo</td><td></td>

<td> 12</td><td> 13</td><td> 14</td><td rowspan="19"></td>

<td>Sugar (sucrose)</td><td>2328.6 kg</td><td>2495.1 kg</td><td>2328.6 kg</td><td>2661.5 kg</td><td>2328.6 kg</td>

<td>Soy oil</td><td>1908.3 kg</td><td>1908.3 kg</td><td>1908.3 kg</td><td>1908.3 kg</td><td>1908.3 kg</td>

<td>Coconut oil</td><td>1844.7 kg</td><td>1844.7 kg</td><td>1844.7 kg</td><td>1844.7 kg</td><td>1844.7 kg</td>

<td>5% hydroxide potassium</td><td>600 kg</td><td>600 kg</td><td>600 kg</td><td>600 kg</td><td>600 kg</td>

<td>Potassium citrate # 1, # 2</td><td>279.8 kg</td><td>279.8 kg</td><td>279.8 kg</td><td>279.8 kg</td><td>279.8 kg</td>

<td>Tribasic calcium phosphate</td><td>252.5 kg</td><td>252.5 kg</td><td>252.5 kg</td><td>252.5 kg</td><td>252.5 kg</td>

<td>Dibasic calcium phosphate</td><td>147.6 kg</td><td>147.6 kg</td><td>147.6 kg</td><td>147.6 kg</td><td>147.6 kg</td>

<td>Sodium chloride</td><td>76.2 kg</td><td>76.2 kg</td><td>76.2 kg</td><td>76.2 kg</td><td>76.2 kg</td>

<td>Magnesium chloride</td><td>58.8 kg</td><td>58.8 kg</td><td>58.8 kg</td><td>58.8 kg</td><td>58.8 kg</td>

<td>L-methionine</td><td>36.2 kg</td><td>36.2 kg</td><td>36.2 kg</td><td>36.2 kg</td><td>36.2 kg</td>

<td>Ascorbic acid</td><td>36.1 kg</td><td>36.1 kg</td><td>36.1 kg</td><td>36 1 kg</td><td>36.1 kg</td>

<td>Premix vitamin / mineral / taurine</td><td>32.5 kg</td><td>32.5 kg</td><td>32.5 kg</td><td>32.5 kg</td><td>32.5 kg</td>

<td>Choline Chloride</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td>

<td>Vitamin A premix, D3, E, &amp; K1</td><td>8.7 kg</td><td>8.7 kg</td><td>8.7 kg</td><td>8.7 kg</td><td>8.7 kg</td>

<td>Ascorbyl Palmitate</td><td>8.1 kg</td><td>8.1 kg</td><td>8.1 kg</td><td>8.1 kg</td><td>8.1 kg</td>

<td>Ferrous sulfate</td><td>6.9 kg</td><td>6.9 kg</td><td>6.9 kg</td><td>6.9 kg</td><td>6.9 kg</td>

<td>Potassium chloride</td><td>4.3 kg</td><td>4.3 kg</td><td>4.3 kg</td><td>4.3 kg</td><td>4.3 kg</td>

<td>Tocopherols mixer</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td>

<td>Ingredient</td><td colspan="5">Amounts per INDUSTRIAL</td><td rowspan="6"> , -</td>

<td rowspan="2"></td><td rowspan="2">Example 10</td><td rowspan="2">Example eleven</td><td>Example</td><td>Example</td><td rowspan="2">Example ^ 14</td>

<td> 12</td><td> 13</td>

<td>L-carnitine</td><td>2.5 kg</td><td>2.5 kg</td><td>2.5 kg</td><td>2.5 kg</td><td>2.5 kg</td>

<td>Beta-carotene in oil vegetable</td><td>28.3 g</td><td>28.3 g</td><td>28.3 g</td><td>28.3 g</td><td>28.3 g</td>

<td>Potassium iodide</td><td>22.9 g</td><td>22.9 g</td><td>22.9 g</td><td>22.9 g</td><td>22.9 g</td>

Examples 1 5-1 9

<td>Ingredient</td><td colspan="5">Quantities per 1000 kg</td>

<td></td><td>Example fifteen</td><td>Example 16</td><td>Example 17</td><td>Example 18</td><td>Example 19</td>

<td>Corn syrup</td><td>412.6 kg</td><td>419.9 kg</td><td>405.3 kg</td><td>427.3 kg</td><td>397.9 kg</td>

<td>Protein hydrolyzate pea</td><td>146.7 kg</td><td>139.4 kg</td><td>154.0 kg</td><td>132.0 kg</td><td>161.4 kg</td>

<td>High oleic safflower oil</td><td>114.7kg</td><td>114.7 kg</td><td>114.7 kg</td><td>114.7 kg</td><td>114.7kg</td>

<td>Sucrose</td><td>97.9 kg</td><td>97.9 kg</td><td>97.9 kg</td><td>97.9 kg</td><td>97.9 kg</td>

<td>Soy oil</td><td>83.9 kg</td><td>83.9 kg</td><td>83.9 kg</td><td>83.9 kg</td><td>83.9 kg</td>

<td>Coconut oil</td><td>77.3 kg</td><td>77.3 kg</td><td>77.3 kg</td><td>77.3 kg</td><td>77.3 kg</td>

<td>Fructo-oligosaccharides</td><td>16.2 kg</td><td>16.2 kg</td><td>16.2 kg</td><td>16.2 kg</td><td>16.2 kg</td>

<td>Potassium citrate</td><td>15.3 kg</td><td>15.3 kg</td><td>15.3 kg</td><td>15.3 kg</td><td>15.3 kg</td>

<td>Tricalcium phosphate</td><td>11.7 kg</td><td>11.7 kg</td><td>11.7 kg</td><td>11.7 kg</td><td>11.7 kg</td>

<td>Dibasic calcium phosphate</td><td>6.8 kg</td><td>6.8 kg</td><td>6.8 kg</td><td>6.8 kg</td><td>6.8 kg</td>

<td></td><td colspan="4">I</td><td colspan="2">MPI ^^</td>

<td rowspan="20"> 5 10 15 20 25</td><td>Ingredient</td><td colspan="3">Amounts per cacft</td><td colspan="2">INDUSTRIAL Jr **</td>

<td rowspan="2"></td><td rowspan="2">Example fifteen</td><td rowspan="2">Example 16</td><td>Example</td><td>Example</td><td>Example</td>

<td> 17</td><td> 18</td><td> 19</td>

<td>Sodium chloride</td><td>3.4 kg</td><td>3.4 kg</td><td>3.4 kg</td><td>3.4 kg</td><td>3.4 kg</td>

<td>Arachidonic acid</td><td>2.9 kg</td><td>2.9 kg</td><td>2.9 kg</td><td>2.9 kg</td><td>2.9 kg</td>

<td>Magnesium chloride</td><td>2.6 kg</td><td>2.6 kg</td><td>2.6 kg</td><td>2.6 kg</td><td>2.6 kg</td>

<td>L-methionine</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td>

<td>Ascorbic acid</td><td>1.6 kg</td><td>1.6 kg</td><td>1.6 kg</td><td>1.6 kg</td><td>1.6 kg</td>

<td>Vitamin / Mineral / Taurine Premix</td><td>1.4 kg</td><td>1.4 kg</td><td>1.4 kg</td><td>1.4 kg</td><td>1.4 kg</td>

<td>Docosahexaenoic acid</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td>

<td>Choline Chloride</td><td>507.7 g</td><td>507.7 g</td><td>507.7 g</td><td>507.7 g</td><td>507.7 g</td>

<td>Vitamin premix ADEK</td><td>372.6 g</td><td>372.6 g</td><td>372.6 g</td><td>372.6 g</td><td>372.6 g</td>

<td>Ascorbyl Palmitate</td><td>357.5 g</td><td>357.5 g</td><td>357.5 g</td><td>357.5 g</td><td>357.5 g</td>

<td>Ferrous sulfate</td><td>305.3 g</td><td>305.3 g</td><td>305.3 g</td><td>305.3 g</td><td>305.3 g</td>

<td>Potassium chloride</td><td>198.5 g</td><td>198.5 g</td><td>198.5 g</td><td>198.5 g</td><td>198.5 g</td>

<td>Premix carotenoids</td><td>187.4 g</td><td>187.4 g</td><td>187.4 g</td><td>187.4 g</td><td>187.4 g</td>

<td>Tocopherols mixer</td><td>157.2 g</td><td>157.2 g</td><td>157.2 g</td><td>157.2 g</td><td>157.2 g</td>

<td>L-carnitine</td><td>107.8 g</td><td>107.8 g</td><td>107.8 g</td><td>107.8 g</td><td>107.8 g</td>

<td>Potassium iodide</td><td>10g</td><td>1.0g</td><td>1-0 g</td><td>1.0g</td><td>1.0g</td>

<td>5% KOH</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

<img file="MX347477B_D0022.tif" />

Ex mplos 20-24

<td>Ingredient</td><td></td><td colspan="3">Quantities p^nr 4nnn iqj</td><td></td>

<td></td><td>Example twenty</td><td>Example twenty-one</td><td>Example 22</td><td>Example 2. 3</td><td>Example 24</td>

<td>Corn syrup</td><td>412.6 kg</td><td>412.6 kg</td><td>412.6 kg</td><td>412.6 kg</td><td>412.6 kg</td>

<td>Protein hydrolyzate pea</td><td>132.0 kg</td><td>117.4 kg</td><td>102.7 kg</td><td>88.0 kg</td><td>73.35 kg</td>

<td>Soy protein</td><td>14.7 kg</td><td>29.3 kg</td><td>44.0 kg</td><td>58.7 kg</td><td>73 35 kg</td>

<td>High oleic safflower oil</td><td>114.7 kg</td><td>114.7 kg</td><td>114.7 kg</td><td>114.7 kg</td><td>114.7 kg</td>

<td>Sucrose</td><td>97.9 kg</td><td>97.9 kg</td><td>97.9 kg</td><td>97.9 kg</td><td>97.9 kg</td>

<td>Soy oil</td><td>83.9 kg</td><td>83.9 kg</td><td>83.9 kg</td><td>83.9 kg</td><td>83.9 kg</td>

<td>Coconut oil</td><td>77.3 kg</td><td>77.3 kg</td><td>77.3 kg</td><td>77.3 kg</td><td>77.3 kg</td>

<td>Fructo-oligosaccharides</td><td>16.2 kg</td><td>16.2 kg</td><td>16.2 kg</td><td>16.2 kg</td><td>16.2 kg</td>

<td>Potassium citrate</td><td>15.3 kg</td><td>15.3 kg</td><td>15.3 kg</td><td>15.3 kg</td><td>15.3 kg</td>

<td>Tricalcium phosphate</td><td>11.7 kg</td><td>11.7 kg</td><td>11.7 kg</td><td>11.7 kg</td><td>11.7 kg</td>

<td>Dibasic calcium phosphate</td><td>6.8 kg</td><td>6.8 kg</td><td>6.8 kg</td><td>6.8 kg</td><td>6.8 kg</td>

<td>Sodium chloride</td><td>3.4 kg</td><td>3.4 kg</td><td>3.4 kg</td><td>3.4 kg</td><td>3.4 kg</td>

<td>Arachidonic acid</td><td>2.9 kg</td><td>2.9 kg</td><td>2.9 kg</td><td>2.9 kg</td><td>2.9 kg</td>

<td>Magnesium chloride</td><td>2.6 kg</td><td>2.6 kg</td><td>2.6 kg</td><td>2.6 kg</td><td>2.6 kg</td>

<td>L-methionine</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td>

<td>Ascorbic acid</td><td>1.6 kg</td><td>1.6 kg</td><td>1.6 kg</td><td>1.6 kg</td><td>1.6 kg</td>

<td>Vitamin / Mineral / Taurine Premix</td><td>1.4 kg</td><td>1.4 kg</td><td>1.4 kg</td><td>1.4 kg</td><td>1.4 kg</td>

MEXICAN INSTITUTE ...)

<td>Ingredient</td><td colspan="5">Quantities per box féeOgt</td>

<td></td><td>Example twenty</td><td>Example twenty-one</td><td>Example- 22</td><td>Fiomnln 2. 3</td><td>- Example. 24</td>

<td>Docosahexaenoic acid</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td>

<td>Choline Chloride</td><td>507.7 g</td><td>507.7 g</td><td>507.7 g</td><td>507.7 g</td><td>507.7 g</td>

<td>Vitamin premix ADEK</td><td>372.6 g</td><td>372.6 g</td><td>372.6 g</td><td>372.6 g</td><td>372.6 g</td>

<td>Ascorbyl Palmitate</td><td>357.5 g</td><td>357.5 g</td><td>357.5 g</td><td>357.5 g</td><td>357.5 g</td>

<td>Ferrous sulfate</td><td>305.3 g</td><td>305.3 g</td><td>305.3 g</td><td>305.3 g</td><td>305.3 g</td>

<td>Potassium chloride</td><td>198.5 g</td><td>198.5 g</td><td>198.5 g</td><td>198.5 g</td><td>198.5 g</td>

<td>Carotenoid premix</td><td>187.4 g</td><td>187.4 g</td><td>187.4 g</td><td>187.4 g</td><td>187.4 g</td>

<td>Tocopherols mixer</td><td>157.2 g</td><td>157.2 g</td><td>157.2 g</td><td>157.2 g</td><td>157.2 g</td>

<td>L-carnitine</td><td>107.8 g</td><td>107.8 g</td><td>107.8 g</td><td>107.8 g</td><td>107.8 g</td>

<td>Potassium iodide</td><td>1-0g</td><td>1.0 g</td><td>1.0 g</td><td>1.0 g</td><td>1.0g</td>

<td>5% KOH</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

Examples 25-29

<td>Ingredients</td><td colspan="5">Amounts per 22679.62 kg</td>

<td></td><td>Example 25</td><td>Example 26</td><td>Example 27</td><td>Example 28</td><td>Example 29</td>

<td>Maltodextrin</td><td>7595 kg</td><td>7793.9 kg</td><td>7396.1 kg</td><td>7992.8 kg</td><td>7197.2 kg</td>

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INSTITUTE-MEXICAN--

<td>Ingredients</td><td colspan="5">- Amounts per caCTÍ® ^</td>

<td>Protein hydrolyzate pea</td><td>3978 kg</td><td>3779.1 kg</td><td>4176.9 kg</td><td>3580.2 kg</td><td>4375.8 kg</td>

<td>Sucrose</td><td>3288 kg</td><td>3288 kg</td><td>3288 kg</td><td>3288 kg</td><td>3288 kg</td>

<td>High oleic safflower oil</td><td>2204 kg</td><td>2204 kg</td><td>2204 kg</td><td>2204 kg</td><td>2204 kg</td>

<td>Fractionated coconut oil (MCT)</td><td>2162 kg</td><td>2162 kg</td><td>2162 kg</td><td>2162 kg</td><td>2162 kg</td>

<td>Soy oil</td><td>1825 kg</td><td>1825 kg</td><td>1825 kg</td><td>1825 kg</td><td>1825 kg</td>

<td>Tricalcium phosphate micronized</td><td>329 kg</td><td>329 kg</td><td>329 kg</td><td>329 kg</td><td>329 kg</td>

<td>Panodan FDPK</td><td>326 kg</td><td>326 kg</td><td>326 kg</td><td>326 kg</td><td>326 kg</td>

<td>Potassium citrate</td><td>276 kg</td><td>276 kg</td><td>276 kg</td><td>276 kg</td><td>276 kg</td>

<td>Xanthan gum extra fine</td><td>128 kg</td><td>128 kg</td><td>128 kg</td><td>128 kg</td><td>128 kg</td>

<td>Magnesium chloride</td><td>69.6 kg</td><td>69.6 kg</td><td>69.6 kg</td><td>69.6 kg</td><td>69.6 kg</td>

<td>Distilled Monoglycerides</td><td>65.2 kg</td><td>65.2 kg</td><td>65.2 kg</td><td>65.2 kg</td><td>65.2 kg</td>

<td>Arachidonic acid (AA)</td><td>60.0 kg</td><td>60.0 kg</td><td>60.0 kg</td><td>60.0 kg</td><td>60.0 kg</td>

<td>Sodium chloride</td><td>59.2 kg</td><td>59.2 kg</td><td>59.2 kg</td><td>59.2 kg</td><td>59.2 kg</td>

<td>Ascorbic acid</td><td>51.2 kg</td><td>51.2 kg</td><td>51.2 kg</td><td>51.2 kg</td><td>51.2 kg</td>

<td>L-cystine dihydrochloride</td><td>46.6 kg</td><td>46.6 kg</td><td>46.6 kg</td><td>46.6 kg</td><td>46.6 kg</td>

<td>Calcium carbonate</td><td>33.7 kg</td><td>33.7 kg</td><td>33.7 kg</td><td>33.7 kg</td><td>33.7 kg</td>

<td>Vitamin / Mineral / Taurine Premix</td><td>31.1 kg</td><td>31.1 kg</td><td>31.1 kg</td><td>31.1 kg</td><td>31.1 kg</td>

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--DE.LA_ggÜFlEEXB - 65 ..... 0. &gt;.

<td>Ingredients</td><td colspan="5">msTrnjTo Mexican Amounts for each</td>

<td rowspan="2">Docosahexaenoic acid (DHA)</td><td rowspan="2">22.5 kg</td><td rowspan="2">22.5 kg</td><td></td><td></td><td></td>

<td>22.5 kg</td><td>22.5 kg</td><td>22.5 kg</td>

<td>L-tyrosine</td><td>19.7 kg</td><td>19.7 kg</td><td>19.7 kg</td><td>19.7 kg</td><td>19.7 kg</td>

<td>Potassium chloride</td><td>16.7 kg</td><td>16.7 kg</td><td>16.7 kg</td><td>16.7 kg</td><td>16.7 kg</td>

<td>Choline Chloride</td><td>13.0 kg</td><td>13.0 kg</td><td>13.0 kg</td><td>13.0 kg</td><td>13.0 kg</td>

<td>L-Tryptophan</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td>

<td>Ferrous sulfate</td><td>10.9 kg</td><td>10.9 kg</td><td>10.9 kg</td><td>10.9 kg</td><td>10.9 kg</td>

<td>Vitamin A premix, D3, E, &amp; K1</td><td>8.53 kg</td><td>8.53 kg</td><td>8.53 kg</td><td>8.53 kg</td><td>8.53 kg</td>

<td>Ascorbyl Palmitate</td><td>5.34 kg</td><td>5.34 kg</td><td>5.34 kg</td><td>5.34 kg</td><td>5.34 kg</td>

<td>Food Grade Antioxidant Tocopherol-2</td><td>2.67 kg</td><td>2.67 kg</td><td>2.67 kg</td><td>2.67 kg</td><td>2.67 kg</td>

<td>L-carnitine</td><td>2.52 kg</td><td>2.52 kg</td><td>2.52 kg</td><td>2.52 kg</td><td>2.52 kg</td>

<td>Potassium iodide</td><td>33.8 g</td><td>33.8 g</td><td>33.8 g</td><td>33.8 g</td><td>33.8 g</td>

<td>5% potassium hydroxide (processing aid)</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

Examples 30-34

<td>Ingredients</td><td colspan="5">Amounts per 22679.62 kg</td>

<td></td><td>Example 30</td><td>Example 31</td><td>Example 32</td><td>Example 33</td><td>Example 3. 4</td>

<td>Maltodextrin</td><td>7595 kg</td><td>7595 kg</td><td>7595 kg</td><td>7595 kg</td><td>7595 kg</td>

<td>Ingredients</td><td colspan="5">--------------- Id -1 ---------- Amounts per 2267 &amp; i82^rkg</td>

<td></td><td>Example 30</td><td>Example 31</td><td>Example + e— 32</td><td>Example 33</td><td>- Ejompte— 3. 4</td>

<td>Pea protein hydrolyzate</td><td>3580.2 kg</td><td>3182.4 kg</td><td>2784.6 kg</td><td>2386.8 kg</td><td>1989 kg</td>

<td>Casein hydrolyzate</td><td>397.8 kg</td><td>795.6 kg</td><td>1193.4 kg</td><td>1591.2 kg</td><td>1989 kg</td>

<td>Sucrose</td><td>3288 kg</td><td>3288 kg</td><td>3288 kg</td><td>3288 kg</td><td>3288 kg</td>

<td>High oleic safflower oil</td><td>2204 kg</td><td>2204 kg</td><td>2204 kg</td><td>2204 kg</td><td>2204 kg</td>

<td>Fractionated coconut oil (MCT)</td><td>2162 kg</td><td>2162 kg</td><td>2162 kg</td><td>2162 kg</td><td>2162 kg</td>

<td>Soy oil</td><td>1825 kg</td><td>1825 kg</td><td>1825 kg</td><td>1825 kg</td><td>1825 kg</td>

<td>Tricalcium phosphate micronized</td><td>329 kg</td><td>329 kg</td><td>329 kg</td><td>329 kg</td><td>329 kg</td>

<td>Panodan FDPK</td><td>326 kg</td><td>326 kg</td><td>326 kg</td><td>326 kg</td><td>326 kg</td>

<td>Potassium citrate</td><td>276 kg</td><td>276 kg</td><td>276 kg</td><td>276 kg</td><td>276 kg</td>

<td>Xanthan gum extra fine</td><td>128 kg</td><td>128 kg</td><td>128 kg</td><td>128 kg</td><td>128 kg</td>

<td>Magnesium chloride</td><td>69.6 kg</td><td>69.6 kg</td><td>69.6 kg</td><td>69.6 kg</td><td>69.6 kg</td>

<td>Distilled Monoglycerides</td><td>65.2 kg</td><td>65.2 kg</td><td>65.2 kg</td><td>65.2 kg</td><td>65.2 kg</td>

<td>Arachidonic acid (AA)</td><td>60.0 kg</td><td>60.0 kg</td><td>60.0 kg</td><td>60.0 kg</td><td>60.0 kg</td>

<td>Sodium chloride</td><td>59.2 kg</td><td>59.2 kg</td><td>59.2 kg</td><td>59.2 kg</td><td>59.2 kg</td>

<td>Ascorbic acid</td><td>51.2 kg</td><td>51.2 kg</td><td>51.2 kg</td><td>51.2 kg</td><td>51.2 kg</td>

<td>L-cystine dihydrochloride</td><td>46.6 kg</td><td>46.6 kg</td><td>46.6 kg</td><td>46.6 kg</td><td>46.6 kg</td>

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<td>Ingredients</td><td colspan="5">Amounts for each</td>

<td rowspan="2"></td><td rowspan="2">Example 30</td><td rowspan="2">Example 31</td><td>Example "</td><td>-Example</td><td>Eiemolo</td>

<td> 32</td><td> 33</td><td> 34</td>

<td>Calcium carbonate</td><td>33.7 kg</td><td>33.7 kg</td><td>33.7 kg</td><td>33.7 kg</td><td>33.7 kg</td>

<td>Premix vitamin / mineral / taurine</td><td>31.1 kg</td><td>31.1 kg</td><td>31.1 kg</td><td>31.1 kg</td><td>31.1 kg</td>

<td>Docosahexaenoic acid (DHA)</td><td>22.5 kg</td><td>22.5 kg</td><td>22.5 kg</td><td>22.5 kg</td><td>22.5 kg</td>

<td>L-tyrosine</td><td>19.7 kg</td><td>19.7 kg</td><td>19.7 kg</td><td>19.7 kg</td><td>19.7 kg</td>

<td>Potassium chloride</td><td>16.7 kg</td><td>16.7 kg</td><td>16.7 kg</td><td>16.7 kg</td><td>16.7 kg</td>

<td>Choline Chloride</td><td>13.0 kg</td><td>13.0 kg</td><td>13.0 kg</td><td>13.0 kg</td><td>13.0 kg</td>

<td>L-Tryptophan</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td><td>11.5 kg</td>

<td>Ferrous sulfate</td><td>10.9 kg</td><td>10.9 kg</td><td>10.9 kg</td><td>10.9 kg</td><td>10.9 kg</td>

<td>Vitamin A premix, D3, E, &amp; K1</td><td>8.53 kg</td><td>8.53 kg</td><td>8.53 kg</td><td>8.53 kg</td><td>8.53 kg</td>

<td>Ascorbyl Palmitate</td><td>5.34 kg</td><td>5.34 kg</td><td>5.34 kg</td><td>5.34 kg</td><td>5.34 kg</td>

<td>Tocopherol-2 antioxidant food grade</td><td>2.67 kg</td><td>2.67 kg</td><td>2.67 kg</td><td>2.67 kg</td><td>2.67 kg</td>

<td>L-carnitine</td><td>2.52 kg</td><td>2.52 kg</td><td>2.52 kg</td><td>2.52 kg</td><td>2.52 kg</td>

<td>Potassium iodide</td><td>33.8 g</td><td>33.8 g</td><td>33.8 g</td><td>33.8 g</td><td>33.8 g</td>

<td>5% potassium hydroxide (processing aid)</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

Examples 35-54

Examples 35-54 illustrate the modal¡dwd ^^ 4a

INDUSTRIAL nutritional of the present description which includes the pea protein hydrolyzate as at least one source of protein, and in some cases, as the only source of protein, the ingredients of which are listed in the following table. To prepare the nutritional emulsions, at least three separate suspensions (eg CHOMIN suspension, PIW suspension, Is PIF suspension) are prepared and then mixed together, heat treated, homogenized, and standardized. The resulting composition is aseptically packaged in sterilized plastic bottles or retorts. The modalities explained below that include pea protein hydrolysates as the only source of protein are hypoallergenic modalities.

Examples 35-39

<td>Ingredient</td><td colspan="5">Amounts per 1000 Kg</td>

<td></td><td>Example 35</td><td>Example 36</td><td>Example 37</td><td>Example 38</td><td>Example 39</td>

<td>Ingredient water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Corn syrup</td><td>63.5 kg</td><td>63.5 kg</td><td>63.5 kg</td><td>63.5 kg</td><td>63.5 kg</td>

<td>Protein hydrolyzate pea</td><td>18.9 kg</td><td>17.9 kg</td><td>19.8 kg</td><td>17.0 kg</td><td>20.8 kg</td>

<td>Safflower oil with high oleic content</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td>

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<td>Ingredient</td><td colspan="5">-------------—------------------------- of the property— Amounts per 1000 '^ g^ST’^UAL</td>

<td></td><td>Example 35</td><td>Example 36</td><td>Example 37</td><td>Example 38</td><td>Example 39</td>

<td>Sucrose</td><td>12.6 kg</td><td>12.6 kg</td><td>12.6 kg</td><td>12.6 kg</td><td>12.6 kg</td>

<td>Soy oil</td><td>10.5 kg</td><td>10.5 kg</td><td>10.5 kg</td><td>10.5 kg</td><td>10.5 kg</td>

<td>Coconut oil</td><td>10.0 kg</td><td>10.0 kg</td><td>10.0 kg</td><td>10.0 kg</td><td>10.0 kg</td>

<td>Fructo-oligosaccharides</td><td>2.3 kg</td><td>2.3 kg</td><td>2.3 kg</td><td>2.3 kg</td><td>2.3 kg</td>

<td>Calcium citrate</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td>

<td>Micro Tricalcium Phosphate</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td>

<td>Monobasic potassium phosphate</td><td>744.4 g</td><td>744.4 g</td><td>744.4 g</td><td>744.4 g</td><td>744.4 g</td>

<td>Ascorbic acid</td><td>437.2 g</td><td>437.2 g</td><td>437.2 g</td><td>437.2 g</td><td>437.2 g</td>

<td>Potassium citrate</td><td>406.0 g</td><td>406.0 g</td><td>406.0 g</td><td>406.0 g</td><td>406.0 g</td>

<td>Magnesium chloride</td><td> 373.4</td><td> 373.4</td><td> 373.4</td><td> 373.4</td><td> 373.4</td>

<td>Acid oil arachidonic</td><td>366.4 g</td><td>366.4 g</td><td>366.4 g</td><td>366.4 g</td><td>366.4 g</td>

<td>Monoglycends</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td>

<td>Lecithin</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td>

<td>Sodium chloride</td><td>309.5 g</td><td>309.5 g</td><td>309.5 g</td><td>309.5 g</td><td>309.5 g</td>

<td>Potassium Phosphate dibasic</td><td>237.9 g</td><td>237.9 g</td><td>237.9 g</td><td>237.9 g</td><td>237.9 g</td>

<td>L-methionine</td><td>208.0 g</td><td>208.0 g</td><td>208.0 g</td><td>208.0 g</td><td>208.0 g</td>

<td>Seakem RLC</td><td>200.1 g</td><td>200.1 g</td><td>200.1 g</td><td>200.1 g</td><td>200.1 g</td>

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<td>Ingredient</td><td colspan="5">---------------------------—---- INSTITUTO MENGANO— Amounts for each</td>

<td></td><td>Example 35</td><td>Example 36</td><td>Example— 37</td><td>-Example 38</td><td>Sjomplo - 39</td>

<td>Vitamin / Mineral / Taurine Premix</td><td>176.4g</td><td>176.4 g</td><td>176.4 g</td><td>176.4 g</td><td>176.4 g</td>

<td>Potassium chloride</td><td>143.6 g</td><td>143.6 g</td><td>143.6 g</td><td>143.6 g</td><td>143.6 g</td>

<td>Acid oil docosahexaenoic</td><td>135.4 g</td><td>135.4 g</td><td>135.4 g</td><td>135.4g</td><td>135.4 g</td>

<td>Choline Chloride</td><td>71.3 g</td><td>71.3g</td><td>71.3g</td><td>71.3g</td><td>71.3g</td>

<td>Vitamin premix ADEK</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td>

<td>Ferrous sulfate</td><td>44.8 g</td><td>44.8 g</td><td>44.8 g</td><td>44.8 g</td><td>44.8 g</td>

<td>Premix carotenoids</td><td>26.5 g</td><td>26.5 g</td><td>26.5 g</td><td>26.5 g</td><td>26.5 g</td>

<td>L-carnitine</td><td>13.8 g</td><td>13.8 g</td><td>13.8 g</td><td>13.8 g</td><td>13.8 g</td>

<td>Riboflavin</td><td>2.0 g</td><td>2.0 g</td><td>2.0 g</td><td>2.0 g</td><td>2.0 g</td>

<td>Vitamin A Palmitate</td><td>780.0 mg</td><td>780.0 mg</td><td>780.0 mg</td><td>780.0 mg</td><td>780.0 mg</td>

<td>Potassium iodide</td><td>150.0 mg</td><td>150.0 mg</td><td>150.0 mg</td><td>150.0 mg</td><td>150.0 mg</td>

<td>5% KOH</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

Examples 40-44

<td colspan="6"></td><td></td>

<td>Ingredient</td><td colspan="5">Amounts per "0" INDUSTRIAL</td><td rowspan="2"></td>

<td rowspan="2"></td><td rowspan="2">Example 40</td><td rowspan="2">Example 41</td><td>Example</td><td>Example</td><td>Example</td>

<td> 42</td><td> 43</td><td> 44</td><td rowspan="18"></td>

<td>Ingredient water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Corn syrup</td><td>63.5 kg</td><td>63.5 kg</td><td>63.5 kg</td><td>63.5 kg</td><td>63.5 kg</td>

<td>Protein hydrolyzate pea</td><td>17.0 kg</td><td>15.1 kg</td><td>13.2 kg</td><td>11.3kg</td><td>9.45 kg</td>

<td>Soy protein</td><td>1.9 kg</td><td>3.9 kg</td><td>5.8 kg</td><td>7.7 kg</td><td>9.45 kg</td>

<td>High oleic safflower oil</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td>

<td>Sucrose</td><td>12.6 kg</td><td>12.6 kg</td><td>12.6 kg</td><td>12.6 kg</td><td>12.6 kg</td>

<td>Soy oil</td><td>10.5 kg</td><td>10.5 kg</td><td>10.5 kg</td><td>10.5 kg</td><td>10.5 kg</td>

<td>Coconut oil</td><td>10.0 kg</td><td>10.0 kg</td><td>10.0 kg</td><td>10.0 kg</td><td>10.0 kg</td>

<td>Fructo-oligosaccharides</td><td>2.3 kg</td><td>2.3 kg</td><td>2.3 kg</td><td>2.3 kg</td><td>2.3 kg</td>

<td>Calcium citrate</td><td>1.7 kg</td><td>1-7 kg</td><td>1.7 kg</td><td>1.7 kg</td><td>1.7 kg</td>

<td>Micro Tricalcium Phosphate</td><td>1.1 kg</td><td>1.1 kg</td><td>1.1 kg</td><td>11 kg</td><td>1.1 kg</td>

<td>Monobasic potassium phosphate</td><td>744.4 g</td><td>744.4 g</td><td>744.4 g</td><td>744.4 g</td><td>744.4 g</td>

<td>Ascorbic acid</td><td>437.2 g</td><td>437.2 g</td><td>437.2 g</td><td>437.2 g</td><td>437.2 g</td>

<td>Potassium citrate</td><td>406.0 g</td><td>406.0 g</td><td>406.0 g</td><td>406.0 g</td><td>406.0 g</td>

<td>Magnesium chloride</td><td> 373.4</td><td> 373.4</td><td> 373.4</td><td> 373.4</td><td> 373.4</td>

<td>Arachidonic Acid Oil</td><td>366.4 g</td><td>366.4 g</td><td>366.4 g</td><td>366.4 g</td><td>366.4 g</td>

<td>Monoglycerides</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td>

IMPI

ΙΝΓΠΉΠΌ MEXICAN

<td>Ingredient</td><td colspan="5">Amounts per 1000 Kg⁵™*¹</td>

<td></td><td>Example 40</td><td>Example 41</td><td>Example 42</td><td>Example 43</td><td>Example 44</td>

<td>Lecithin</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td><td>350.0 g</td>

<td>Sodium chloride</td><td>309.5 g</td><td>309.5 g</td><td>309.5 g</td><td>309.5 g</td><td>309.5 g</td>

<td>Potassium Phosphate dibasic</td><td>237.9 g</td><td>237.9 g</td><td>237.9 g</td><td>237.9 g</td><td>237.9 g</td>

<td>L-methionine</td><td>208.0 g</td><td>208.0 g</td><td>208.0 g</td><td>208.0 g</td><td>208.0 g</td>

<td>Seakem RLC</td><td>200.1 g</td><td>200.1 g</td><td>200.1 g</td><td>200.1 g</td><td>200.1 g</td>

<td>Premix vitamin / mineral / taurine</td><td>176.4 g</td><td>176.4 g</td><td>176.4 g</td><td>176.4 g</td><td>176.4 g</td>

<td>Potassium chloride</td><td>143.6 g</td><td>143.6 g</td><td>143.6 g</td><td>143.6 g</td><td>143.6 g</td>

<td>Acid oil docosahexaenoic</td><td>135.4 g</td><td>135.4 g</td><td>135.4 g</td><td>135.4g</td><td>135.4 g</td>

<td>Choline Chloride</td><td>71.3g</td><td>71.3 g</td><td>71.3 g</td><td>71.3g</td><td>71.3g</td>

<td>Vitamin premix ADEK</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td>

<td>Ferrous sulfate</td><td>44.8 g</td><td>44.8 g</td><td>44.8 g</td><td>44.8 g</td><td>44.8 g</td>

<td>Premix carotenoids</td><td>26.5 g</td><td>26.5 g</td><td>26.5 g</td><td>26.5 g</td><td>26.5 g</td>

<td>L-carnitine</td><td>13.8g</td><td>13.8 g</td><td>13.8 g</td><td>13.8 g</td><td>13.8g</td>

<td>Riboflavin</td><td>2.0 g</td><td>2.0 g</td><td>2.0 g</td><td>2.0 g</td><td>2.0 g</td>

<td>Vitamin A Palmitate</td><td>780.0 mg</td><td>780.0 mg</td><td>780.0 mg</td><td>780.0 mg</td><td>780.0 mg</td>

<td>Potassium iodide</td><td>150.0 mg</td><td>150.0 mg</td><td>150.0 mg</td><td>150.0 mg</td><td>150.0 mg</td>

IMPIOUS

MEXICAN INSTITUTE

<td>Ingredient</td><td colspan="5">-------------------------------------------------- pe the owner Amounts per 1OOtPK§ *^IAL</td>

<td></td><td>Example 40</td><td>Example 41</td><td>Example 42</td><td>Example 43</td><td>"Tjémpnr * 44</td>

<td>5% KOH</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

Examples 45-49

<td>Ingredients</td><td colspan="5">Amounts per 45359.24 kg</td>

<td></td><td>Example Four. Five</td><td>Example 46</td><td>Example 47</td><td>Example 48</td><td>Example 49</td>

<td>Ingredient water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Sucrose</td><td>2007 kg</td><td>2007 kg</td><td>2007 kg</td><td>2007 kg</td><td>2007 kg</td>

<td>Protein hydrolyzate pea</td><td>1025 kg</td><td>973.8 kg</td><td>1076.2 kg</td><td>922.5 kg</td><td>1127.5 kg</td>

<td>Tapioca starch modified</td><td>989 kg</td><td>989 kg</td><td>989 kg</td><td>989 kg</td><td>989 kg</td>

<td>Safflower oil</td><td>644 kg</td><td>644 kg</td><td>644 kg</td><td>644 kg</td><td>644 kg</td>

<td>Coconut oil fractional</td><td>568 kg</td><td>568 kg</td><td>568 kg</td><td>568 kg</td><td>568 kg</td>

<td>Soy oil</td><td>479 kg</td><td>479 kg</td><td>479 kg</td><td>479 kg</td><td>479 kg</td>

<td>5% KOH (processing aid)</td><td>187 kg</td><td>187 kg</td><td>187 kg</td><td>187 kg</td><td>187 kg</td>

<td>Calcium citrate</td><td>69.9 kg</td><td>69.9 kg</td><td>69.9 kg</td><td>69.9 kg</td><td>69.9 kg</td>

IMPI

IMTITlíTü MEXICANO—.

<td>Ingredients</td><td colspan="5">Amounts for each</td>

<td rowspan="2"></td><td rowspan="2">Example Four. Five</td><td rowspan="2">Example 46</td><td>Example_</td><td>Example</td><td>Example</td>

<td> 47</td><td> 48</td><td> 49</td>

<td>Dibasic calcium phosphate</td><td>48.1 kg</td><td>48.1 kg</td><td>48.1 kg</td><td>48.1 kg</td><td>48.1 kg</td>

<td>Carrageenan</td><td>45.4 kg</td><td>45.4 kg</td><td>45.4 kg</td><td>45.4 kg</td><td>45.4 kg</td>

<td>Potassium Phosphate dibasic</td><td>26.2 kg</td><td>26.2 kg</td><td>26.2 kg</td><td>26.2 kg</td><td>26.2 kg</td>

<td>Ascorbic acid</td><td>24.2 kg</td><td>24.2 kg</td><td>24.2 kg</td><td>24.2 kg</td><td>24.2 kg</td>

<td>Magnesium chloride</td><td>18.6 kg</td><td>18.6 kg</td><td>18.6 kg</td><td>18.6 kg</td><td>18.6 kg</td>

<td>M. Alpina oil (ARA oil)</td><td>17.9 kg</td><td>17.9 kg</td><td>17.9 kg</td><td>17.9 kg</td><td>17.9 kg</td>

<td>Potassium citrate</td><td>15.9 kg</td><td>15.9 kg</td><td>15.9 kg</td><td>15.9 kg</td><td>15.9 kg</td>

<td>Sodium chloride</td><td>14.9 kg</td><td>14.9 kg</td><td>14.9 kg</td><td>14.9 kg</td><td>14.9 kg</td>

<td>Calcium hydroxide</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td>

<td>L-cystine dihydrochloride</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td>

<td>Potassium chloride</td><td>11.2 kg</td><td>11.2 kg</td><td>11.2 kg</td><td>11.2 kg</td><td>11.2 kg</td>

<td>Vitamin premix soluble in water</td><td>8.00 kg</td><td>8.00 kg</td><td>8.00 kg</td><td>8.00 kg</td><td>8.00 kg</td>

<td>C. Cohnii oil (DHA oil)</td><td>6.71 kg</td><td>6.71 kg</td><td>6.71 kg</td><td>6.71 kg</td><td>6.71 kg</td>

<td>L-tyrosine</td><td>5.08 kg</td><td>5.08 kg</td><td>5.08 kg</td><td>5.08 kg</td><td>5.08 kg</td>

<td>L-Tryptophan</td><td>4.67 kg</td><td>4.67 kg</td><td>4.67 kg</td><td>4.67 kg</td><td>4.67 kg</td>

<td>Choline Chloride</td><td>3.36 kg</td><td>3.36 kg</td><td>3.36 kg</td><td>3.36 kg</td><td>3.36 kg</td>

WICKED

<td>Ingredients</td><td colspan="5">--------------------------------------------- MSXFGANQ INSTITUTE-- Amounts per 45399 ^^^, ^</td>

<td rowspan="2"></td><td rowspan="2">Example Four. Five</td><td rowspan="2">Example 46</td><td>Example</td><td>Example</td><td>Example</td>

<td> 47</td><td> 48</td><td> 49</td>

<td>Vitamin premix oil soluble</td><td>2.37 kg</td><td>2.37 kg</td><td>2.37 kg</td><td>2.37 kg</td><td>2.37 kg</td>

<td>Ferrous sulfate</td><td>2.32 kg</td><td>2.32 kg</td><td>2.32 kg</td><td>2.32 kg</td><td>2.32 kg</td>

<td>L-carnitine</td><td>650 g</td><td>650 g</td><td>650 g</td><td>650 g</td><td>650 g</td>

<td>Riboflavin</td><td>87.9 g</td><td>87.9 g</td><td>87.9 g</td><td>87.9 g</td><td>87.9 g</td>

<td>Vitamin A Palmitate</td><td>17.6 g</td><td>17.6 g</td><td>17.6 g</td><td>17.6 g</td><td>17.6 g</td>

<td>Potassium iodide</td><td>8.70 g</td><td>8.70 g</td><td>8.70 g</td><td>8.70 g</td><td>8.70 g</td>

Examples 50-54

<td>Ingredients</td><td colspan="5">Amounts per 45359.24 kg</td>

<td></td><td>Example fifty</td><td>Example 51</td><td>Example 52</td><td>Example 53</td><td>Example 54</td>

<td>Ingredient water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Sucrose</td><td>2007 kg</td><td>2007 kg</td><td>2007 kg</td><td>2007 kg</td><td>2007 kg</td>

<td>Protein hydrolyzate pea</td><td>922.5 kg</td><td>820.0 kg</td><td>717.5 kg</td><td>615.0 kg</td><td>512.5 kg</td>

<td>Casein hydrolyzate</td><td>102.5 kg</td><td>205.0 kg</td><td>307.52 kg</td><td>410.0 kg</td><td>512.5 kg</td>

<td>Tapioca starch modified</td><td>989 kg</td><td>989 kg</td><td>989 kg</td><td>989 kg</td><td>989 kg</td>

<td>Safflower oil</td><td>644 kg</td><td>644 kg</td><td>644 kg</td><td>644 kg</td><td>644 kg</td>

IMPI

<td>Ingredients</td><td colspan="5">Quantities per 03 ^^ 559 ^ 2 ^ 0 £ INDUSTRIAL</td>

<td rowspan="2"></td><td rowspan="2">Example fifty</td><td rowspan="2">Example 51</td><td>Example</td><td>Example</td><td>Example</td>

<td> 52</td><td> 53</td><td> 54</td>

<td>Coconut oil fractional</td><td>568 kg</td><td>568 kg</td><td>568 kg</td><td>568 kg</td><td>568 kg</td>

<td>Soy oil</td><td>479 kg</td><td>479 kg</td><td>479 kg</td><td>479 kg</td><td>479 kg</td>

<td>5% KOH (processing aid)</td><td>187 kg</td><td>187 kg</td><td>187 kg</td><td>187 kg</td><td>187 kg</td>

<td>Calcium citrate</td><td>69.9 kg</td><td>69.9 kg</td><td>69.9 kg</td><td>69.9 kg</td><td>69.9 kg</td>

<td>Dibasic calcium phosphate</td><td>48.1 kg</td><td>48.1 kg</td><td>48.1 kg</td><td>48.1 kg</td><td>48.1 kg</td>

<td>Carrageenan</td><td>45.4 kg</td><td>45.4 kg</td><td>45.4 kg</td><td>45.4 kg</td><td>45.4 kg</td>

<td>Potassium Phosphate dibasic</td><td>26.2 kg</td><td>26.2 kg</td><td>26.2 kg</td><td>26.2 kg</td><td>26.2 kg</td>

<td>Ascorbic acid</td><td>24.2 kg</td><td>24.2 kg</td><td>24.2 kg</td><td>24.2 kg</td><td>24.2 kg</td>

<td>Magnesium chloride</td><td>18.6 kg</td><td>18.6 kg</td><td>18.6 kg</td><td>18.6 kg</td><td>18.6 kg</td>

<td>M. Alpina oil (ARA oil)</td><td>17.9 kg</td><td>17.9 kg</td><td>17.9 kg</td><td>17.9 kg</td><td>17.9 kg</td>

<td>Potassium citrate</td><td>15.9 kg</td><td>15.9 kg</td><td>15.9 kg</td><td>15.9 kg</td><td>15.9 kg</td>

<td>Sodium chloride</td><td>14.9 kg</td><td>14.9 kg</td><td>14.9 kg</td><td>14.9 kg</td><td>14.9 kg</td>

<td>Calcium hydroxide</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td><td>14.0 kg</td>

<td>L-cystine dihydrochloride</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td>

<td>Potassium chloride</td><td>11.2 kg</td><td>11.2 kg</td><td>11.2 kg</td><td>11.2 kg</td><td>11.2 kg</td>

<td>Vitamin premix soluble in water</td><td>8.00 kg</td><td>8.00 kg</td><td>8.00 kg</td><td>8.00 kg</td><td>8.00 kg</td>

ΙΜΡ'ϊ ^^ swa »tesáis

<td>Ingredients</td><td colspan="5">Quantities for each WK</td><td rowspan="2"></td>

<td rowspan="2"></td><td rowspan="2">Example fifty</td><td rowspan="2">Example 51</td><td>Example</td><td>Example</td><td>Example</td>

<td> 52</td><td> 53</td><td> 54</td><td rowspan="11"></td>

<td>C. Cohnii oil (DHA oil)</td><td>6.71 kg</td><td>6.71 kg</td><td>6.71 kg</td><td>6.71 kg</td><td>6.71 kg</td>

<td>L-tyrosine</td><td>5.08 kg</td><td>5.08 kg</td><td>5.08 kg</td><td>5.08 kg</td><td>5.08 kg</td>

<td>L-Tryptophan</td><td>4.67 kg</td><td>4.67 kg</td><td>4.67 kg</td><td>4.67 kg</td><td>4.67 kg</td>

<td>Choline Chloride</td><td>3.36 kg</td><td>3.36 kg</td><td>3.36 kg</td><td>3.36 kg</td><td>3.36 kg</td>

<td>Vitamin premix oil soluble</td><td>2.37 kg</td><td>2.37 kg</td><td>2.37 kg</td><td>2.37 kg</td><td>2.37 kg</td>

<td>Ferrous sulfate</td><td>2.32 kg</td><td>2.32 kg</td><td>2.32 kg</td><td>2.32 kg</td><td>2.32 kg</td>

<td>L-carnitine</td><td>650 g</td><td>650 g</td><td>650 g</td><td>650 g</td><td>650 g</td>

<td>Riboflavin</td><td>87.9 g</td><td>87.9 g</td><td>87.9 g</td><td>87.9 g</td><td>87.9 g</td>

<td>Vitamin A Palmitate</td><td>17.6 g</td><td>17.6 g</td><td>17.6 g</td><td>17.6 g</td><td>17.6 g</td>

<td>Potassium iodide</td><td>8.70 g</td><td>8.70 g</td><td>8.70 g</td><td>8.70 g</td><td>8.70 g</td>

Examples 55-64

Examples 55-64 illustrate the nutritional emulsions of the present description, the ingredients of which are listed in the following table. To prepare nutritional emulsions, at least three separate suspensions (e.g., CHO-MIN suspension, PIW suspension, and PIF suspension) are prepared and then mixed together, heat treated, homogenized, and they are standardized. The resulting composition is aseptically packaged in sterilized plastic bottles or retorts.

<img file="MX347477B_D0023.tif" />

include pea protein hydrolysates as the only protein source are hypoallergenic modalities.

<td>Ingredients</td><td colspan="5">Amounts (kg) per 1000 kg</td>

<td></td><td>Example 55</td><td>Example 56</td><td>Example 57</td><td>Example 58</td><td>Example 59</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Maltodextrin</td><td> 120.0</td><td> 120.0</td><td> 120.0</td><td> 120.0</td><td> 120.0</td>

<td>Sucrose</td><td> 71.38</td><td> 71.38</td><td> 71.38</td><td> 71.38</td><td> 71.38</td>

<td>Protein hydrolyzate pea</td><td> 62.88</td><td> 66.02</td><td> 59.74</td><td> 69.17</td><td> 56.59</td>

<td>Canola oil</td><td> 27.5</td><td> 27.5</td><td> 27.5</td><td> 27.5</td><td> 27.5</td>

<td>Corn oil</td><td> 15.70</td><td> 15.70</td><td> 15.70</td><td> 15.70</td><td> 15.70</td>

<td>Magnesium phosphate</td><td> 1.92</td><td> 1.92</td><td> 1.92</td><td> 1.92</td><td> 1.92</td>

<td>Potassium citrate</td><td> 6.92</td><td> 6.92</td><td> 6.92</td><td> 6.92</td><td> 6.92</td>

<td>Sodium citrate</td><td> 0.903</td><td> 0.903</td><td> 0.903</td><td> 0.903</td><td> 0.903</td>

<td>Lecithin</td><td> 1.50</td><td> 1.50</td><td> 1.50</td><td> 1.50</td><td> 1.50</td>

<td>Sodium tripolyphosphate</td><td> 1.06</td><td> 1.06</td><td> 1.06</td><td> 1.06</td><td> 1.06</td>

<td>Potassium Phosphate dibasic</td><td> 0.730</td><td> 0.730</td><td> 0.730</td><td> 0.730</td><td> 0.730</td>

<td>Potassium chloride</td><td> 1.04</td><td> 1.04</td><td> 1.04</td><td> 1.04</td><td> 1.04</td>

<td>Ascorbic acid</td><td> 0.235</td><td> 0.235</td><td> 0.235</td><td> 0.235</td><td> 0.235</td>

<td>Carrageenan</td><td> 0.150</td><td> 0.150</td><td> 0.150</td><td> 0.150</td><td> 0.150</td>

<td>Potassium hydroxide</td><td> 0.136</td><td> 0.136</td><td> 0.136</td><td> 0.136</td><td> 0.136</td>

<td></td><td></td><td></td><td colspan="2">TMPT</td><td></td><td></td>

<td>Ingredients</td><td></td><td colspan="3">Quantities (kg) INDUSTRIAL</td><td></td><td></td>

<td></td><td>Example</td><td>Example</td><td>Example</td><td>Example</td><td>Example</td><td></td>

<td></td><td> 55</td><td> 56</td><td> 57</td><td> 58</td><td> 59</td><td></td>

<td>TM / UTM premix</td><td> 0.1684</td><td> 0.1684</td><td> 0.1684</td><td> 0.1684</td><td> 0.1684</td><td></td>

<td>Gellan gum</td><td> 0.050</td><td> 0.050</td><td> 0.050</td><td> 0.050</td><td> 0.050</td><td></td>

<td>Vitamin premix A, D, E</td><td> 0.0758</td><td> 0.0758</td><td> 0.0758</td><td> 0.0758</td><td> 0.0758</td><td></td>

<td>Vitamin premix soluble in water</td><td> 0.0728</td><td> 0.0728</td><td> 0.0728</td><td> 0.0728</td><td> 0.0728</td><td></td>

<td>Potassium iodide</td><td> 0.00022</td><td> 0.00022</td><td> 0.00022</td><td> 0.00022</td><td> 0.00022</td><td></td>

<td>Chromium Chloride</td><td> 0.000217</td><td> 0.000217</td><td> 0.000217</td><td> 0.000217</td><td> 0.000217</td><td></td>

<td>Flavoring</td><td> 3.3</td><td> 3.3</td><td> 3.3</td><td> 3.3</td><td> 3.3</td><td></td>

<td>Ingredients</td><td colspan="5">Amounts (kg) per 1000 kg</td>

<td></td><td>Example 60</td><td>Example 61</td><td>Example 62</td><td>Example 63</td><td>Example 64</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Maltodextrin</td><td> 120.0</td><td> 120.0</td><td> 120.0</td><td> 120.0</td><td> 120.0</td>

<td>Sucrose</td><td> 71.38</td><td> 71.38</td><td> 71.38</td><td> 71.38</td><td> 71.38</td>

<td>Protein concentrate milk</td><td> 9.33</td><td> 7.46</td><td> 5.60</td><td> 3.73</td><td> 1.87</td>

<td>Canola oil</td><td> 27.5</td><td> 27.5</td><td> 27.5</td><td> 27.5</td><td> 27.5</td>

<td>Protein hydrolyzate pea</td><td> 31.50</td><td> 39.61</td><td> 41.82</td><td> 55.34</td><td> 50.93</td>

<td>Ingredients</td><td colspan="5">Amounts (kg) per £ ^ MB_TOKg OF INDUSTRIAL PROPERTY</td>

<td rowspan="2"></td><td rowspan="2">Example 60</td><td rowspan="2">Example 61</td><td>Example</td><td>Example</td><td>Example</td>

<td> 62</td><td> 63</td><td> 64</td>

<td>Sodium caseinate</td><td> 13.25</td><td> 10.67</td><td> 8.00</td><td> 5.34</td><td> 2.67</td>

<td>Soy protein concentrate</td><td> 7.00</td><td> 5.60</td><td> 4.22</td><td> 2.81</td><td> 1.40</td>

<td>Corn oil</td><td> 15.70</td><td> 15.70</td><td> 15.70</td><td> 15.70</td><td> 15.70</td>

<td>Whey protein concentrate</td><td> 1.75</td><td> 1.40</td><td> 1.05</td><td> 0.70</td><td> 0.35</td>

<td>Magnesium phosphate</td><td> 1.92</td><td> 1.92</td><td> 1.92</td><td> 1.92</td><td> 1.92</td>

<td>Potassium citrate</td><td> 6.92</td><td> 6.92</td><td> 6.92</td><td> 6.92</td><td> 6.92</td>

<td>Sodium citrate</td><td> 0.903</td><td> 0.903</td><td> 0.903</td><td> 0.903</td><td> 0.903</td>

<td>Lecithin</td><td> 1.50</td><td> 1.50</td><td> 1.50</td><td> 1.50</td><td> 1.50</td>

<td>Sodium tripolyphosphate</td><td> 1.06</td><td> 1.06</td><td> 1.06</td><td> 1.06</td><td> 1.06</td>

<td>Potassium Phosphate dibasic</td><td> 0.730</td><td> 0.730</td><td> 0.730</td><td> 0.730</td><td> 0.730</td>

<td>Potassium chloride</td><td> 1.04</td><td> 1.04</td><td> 1.04</td><td> 1.04</td><td> 1.04</td>

<td>Ascorbic acid</td><td> 0.235</td><td> 0.235</td><td> 0.235</td><td> 0.235</td><td> 0.235</td>

<td>Carrageenan</td><td> 0.150</td><td> 0.150</td><td> 0.150</td><td> 0.150</td><td> 0.150</td>

<td>Potassium hydroxide</td><td> 0.136</td><td> 0.136</td><td> 0.136</td><td> 0.136</td><td> 0.136</td>

<td>TM / UTM premix</td><td> 0.1684</td><td> 0.1684</td><td> 0.1684</td><td> 0.1684</td><td> 0.1684</td>

<td>Gellan gum</td><td> 0.050</td><td> 0.050</td><td> 0.050</td><td> 0.050</td><td> 0.050</td>

<td>Vitamin premix A, D, E</td><td> 0.0758</td><td> 0.0758</td><td> 0.0758</td><td> 0.0758</td><td> 0.0758</td>

<td>Ingredients</td><td colspan="3">I Amounts (kg) per</td><td colspan="2">INDUSTRIAL</td><td rowspan="7"></td>

<td rowspan="2"></td><td rowspan="2">Example 60</td><td rowspan="2">Example 61</td><td>Example</td><td>Example</td><td>Example</td>

<td> 62</td><td> 63</td><td> 64</td>

<td>Water soluble vitamin premix</td><td> 0.0728</td><td> 0.0728</td><td> 0.0728</td><td> 0.0728</td><td> 0.0728</td>

<td>Potassium iodide</td><td> 0.00022</td><td> 0.00022</td><td> 0.00022</td><td> 0.00022</td><td> 0.00022</td>

<td>Chromium Chloride</td><td> 0.000217</td><td> 0.000217</td><td> 0.000217</td><td> 0.000217</td><td> 0.000217</td>

<td>Flavoring</td><td> 3.3</td><td> 3.3</td><td> 3.3</td><td> 3.3</td><td> 3.3</td>

Examples 65-74

Examples 65-74 illustrate the substantially clear nutritional liquids of the present disclosure, the ingredients of which are listed in the following table. To prepare the nutritional liquids, at least two separate suspensions (eg, CHO-MIN suspension and PIW suspension) are prepared and then mixed together, heat treated, homogenized, and standardized. The resulting composition is aseptically packaged in the sterilized plastic bottles or retorts. The modalities explained below that include pea protein hydrolysates as the only source of protein are hypoallergenic modalities.

IMPI ^ Mexican Institute

<td>Ingredients</td><td colspan="5">Amounts (kg) for each</td>

<td rowspan="2"></td><td rowspan="2">Example 65</td><td rowspan="2">Example 66</td><td>Example -</td><td>-Example</td><td>Example.....</td>

<td> 67</td><td> 68</td><td> 69</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Sucrose</td><td> 109.0</td><td> 109.0</td><td> 0</td><td> 54.5</td><td> 54.5</td>

<td>Isomaltulose</td><td> 0</td><td> 0</td><td> 109.0</td><td> 54.5</td><td> 54.5</td>

<td>Protein hydrolyzate pea</td><td> 42.90</td><td> 45.05</td><td> 40.76</td><td> 47.19</td><td> 38.61</td>

<td>Phosphoric acid</td><td> 2.0</td><td> 2.0</td><td> 2.0</td><td> 2.0</td><td> 2.0</td>

<td>Citric acid</td><td> 1.4</td><td> 1.4</td><td> 1.4</td><td> 1.4</td><td> 1.4</td>

<td>Apple flavoring N&amp;A</td><td>700 g</td><td>700 g</td><td>700 g</td><td>700 g</td><td>700 g</td>

<td>Ascorbic acid</td><td>535 g</td><td>535 g</td><td>535 g</td><td>535 g</td><td>535 g</td>

<td>Liquid caramel color</td><td>400 g</td><td>400 g</td><td>400 g</td><td>400 g</td><td>400 g</td>

<td>UTM / TM premix</td><td>230 g</td><td>230 g</td><td>230 g</td><td>230 g</td><td>230 g</td>

<td>Sulfate Monohydrate zinc*</td><td>52.1 g</td><td>52.1 g</td><td>52.1 g</td><td>52.1 g</td><td>52.1 g</td>

<td>Ferrous sulfate*</td><td>40.8 g</td><td>40.8 g</td><td>40.8 g</td><td>40.8 g</td><td>40.8 g</td>

<td>Citric acid*</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td>

<td>Manganese sulphate*</td><td> 13.0</td><td> 13.0</td><td> 13.0</td><td> 13.0</td><td> 13.0</td>

<td>Copper sulphate*</td><td>7.1 g</td><td>7.1 g</td><td>7.1 g</td><td>7.1 g</td><td>7.1 g</td>

<td>Chromium chloride *</td><td>430 mg</td><td>430 mg</td><td>430 mg</td><td>430 mg</td><td>430 mg</td>

<td>Sodium molybdate *</td><td>339 mg</td><td>339 mg</td><td>339 mg</td><td>339 mg</td><td>339 mg</td>

<td>Sodium selenate *</td><td>146 mg</td><td>146 mg</td><td>146 mg</td><td>146 mg</td><td>146 mg</td>

IMPIOUS

<td>Ingr teeth</td><td colspan="5">Amounts (kg) per caddl ^ O ^ Z</td>

<td rowspan="2"></td><td rowspan="2">Example 65</td><td rowspan="2">Example 66</td><td>Example-</td><td>-Example</td><td>Eiemnio</td>

<td> 67</td><td> 68</td><td> 69</td>

<td>ADEK Premix disperse him in water</td><td>178 g</td><td>178 g</td><td>178 g</td><td>178 g</td><td>178g</td>

<td>Di-alpha- acetate tocopheryl **</td><td>45.3 g</td><td>45.3 g</td><td>45.3 g</td><td>45.3 g</td><td>45.3 g</td>

<td>Vitamin A palmittate **</td><td>4.2 g</td><td>4.2 g</td><td>4.2 g</td><td>4.2 g</td><td>4.2 g</td>

<td>Phylloquinone **</td><td>127 mg</td><td>127 mg</td><td>127 mg</td><td>127 mg</td><td>127 mg</td>

<td>Vitamin D3 **</td><td>23 mg</td><td>23 mg</td><td>23 mg</td><td>23 mg</td><td>23 mg</td>

<td>WSV Premix vitamin</td><td>37.9 g</td><td>37.9 g</td><td>37.9 g</td><td>37.9 g</td><td>37.9 g</td>

<td>Niacinamide ***</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td>

<td>Dicalcium pantothenate ***</td><td>9.2 g</td><td>9.2 g</td><td>9.2 g</td><td>9.2 g</td><td>9.2 g</td>

<td>Chloride hydrochloride thiamine ***</td><td>2.4 g</td><td>2.4 g</td><td>2.4 g</td><td>2.4 g</td><td>2.4 g</td>

<td>Hydrochloride pyridoxine ***</td><td>2.3 g</td><td>2.3 g</td><td>2.3 g</td><td>2.3 g</td><td>2.3 g</td>

'UTM / TM premix; “ADEK Premix;

*** WSV Vitamin Premix

100

WICKED

<td>Ingredients</td><td colspan="5">Quantities (kg) per catfá'WWJi ^ [</td>

<td rowspan="2"></td><td rowspan="2">Example 70</td><td rowspan="2">Example 71</td><td>Example</td><td>Example</td><td>Example</td>

<td> 72</td><td> 73</td><td> 74</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Sucrose</td><td> 109.0</td><td> 109.0</td><td> 0</td><td> 54.5</td><td> 54.5</td>

<td>Isomaltulose</td><td> 0</td><td> 0</td><td> 109.0</td><td> 54.5</td><td> 54.5</td>

<td>Protein hydrolyzate pea</td><td> 21.45</td><td> 27.03</td><td> 28.53</td><td> 37.75</td><td> 34.75</td>

<td>Whey protein isolate</td><td> 18.59</td><td> 12.87</td><td> 11.15</td><td> 6.24</td><td> 3.44</td>

<td>Hydrolyzed casein</td><td> 2.86</td><td> 4.29</td><td> 1.72</td><td> 2.14</td><td> 0.75</td>

<td>Phosphoric acid</td><td> 2.0</td><td> 2.0</td><td> 2.0</td><td> 2.0</td><td> 2.0</td>

<td>Citric acid</td><td> 1.4</td><td> 1.4</td><td> 1.4</td><td> 1.4</td><td> 1.4</td>

<td>Apple flavoring N&amp;A</td><td>700 g</td><td>700 g</td><td>700 g</td><td>700 g</td><td>700 g</td>

<td>Ascorbic acid</td><td>535 g</td><td>535 g</td><td>535 g</td><td>535 g</td><td>535 g</td>

<td>Liquid caramel color</td><td>400 g</td><td>400 g</td><td>400 g</td><td>400 g</td><td>400 g</td>

<td>UTM / TM premix</td><td>230 g</td><td>230 g</td><td>230 g</td><td>230 g</td><td>230 g</td>

<td>Sulfate Monohydrate zinc*</td><td>52.1 g</td><td>52.1 g</td><td>52.1 g</td><td>52.1 g</td><td>52.1 g</td>

<td>Ferrous sulfate*</td><td>40.8 g</td><td>40.8 g</td><td>40.8 g</td><td>40.8 g</td><td>40.8 g</td>

<td>Citric acid*</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td>

<td>Manganese sulphate*</td><td> 13.0</td><td> 13.0</td><td> 13.0</td><td> 13.0</td><td> 13.0</td>

<td>Copper sulphate*</td><td>7.1 g</td><td>7.1 g</td><td>7.1 g</td><td>7.1 g</td><td>7.1 g</td>

101

IMPIAS

<td>Ingr teeth</td><td colspan="5">Amounts (kg) per</td>

<td rowspan="2"></td><td rowspan="2">Example 70</td><td rowspan="2">Example 71</td><td rowspan="2">Example ^ 72</td><td>Example</td><td>Example</td>

<td> 73</td><td> 74</td>

<td>Chromium chloride *</td><td>430 mg</td><td>430 mg</td><td>430 mg</td><td>430 mg</td><td>430 mg</td>

<td>Sodium molybdate *</td><td>339 mg</td><td>339 mg</td><td>339 mg</td><td>339 mg</td><td>339 mg</td>

<td>Sodium selenate *</td><td>146 mg</td><td>146 mg</td><td>146 mg</td><td>146 mg</td><td>146 mg</td>

<td>ADEK Premix water dispersible</td><td>178 g</td><td>178 g</td><td>178 g</td><td>178 g</td><td>178 g</td>

<td>Di-alpha- acetate tocopheryl **</td><td>45.3 g</td><td>45.3 g</td><td>45.3 g</td><td>45.3 g</td><td>45.3 g</td>

<td>Vitamin A Palmitate **</td><td>4.2 g</td><td>4.2 g</td><td>4.2 g</td><td>4.2 g</td><td>4.2 g</td>

<td>Phylloquinone **</td><td>127 mg</td><td>127 mg</td><td>127 mg</td><td>127 mg</td><td>127 mg</td>

<td>Vitamin D3 **</td><td>23 mg</td><td>23 mg</td><td>23 mg</td><td>23 mg</td><td>23 mg</td>

<td>WSV Premix vitamin</td><td>37.9 g</td><td>37.9 g</td><td>37.9 g</td><td>37.9 g</td><td>37.9 g</td>

<td>Niacinamide ***</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td><td>14.2 g</td>

<td>Dicalcium pantothenate ***</td><td>9.2 g</td><td>9.2 g</td><td>9.2 g</td><td>9.2 g</td><td>9.2 g</td>

<td>Chloride hydrochloride thiamine ***</td><td>2.4 g</td><td>2.4 g</td><td>2.4 g</td><td>2.4 g</td><td>2.4 g</td>

<td>Pyridoxine hydrochloride***</td><td>2.3 g</td><td>2.3 g</td><td>2.3 g</td><td>2.3 g</td><td>2.3 g</td>

<td>Riboflavin***</td><td>1.8g</td><td>1.8 g</td><td>1.8g</td><td>1.8 g</td><td>1.8g</td>

<td>Photic acid ***</td><td>350 mg</td><td>350 mg</td><td>350 mg</td><td>350 mg</td><td>350 mg</td>

<td>Biotin ***</td><td>277 mg</td><td>277 mg</td><td>277 mg</td><td>277 mg</td><td>277 mg</td>

102

IMPI

<td>Ingredients</td><td colspan="5">Amounts (kg) per batch&gt; wOñ &lt;kg ' ^r OF THE PROPULSE -1 # INDUSTRIAL</td>

<td rowspan="2"></td><td rowspan="2">Example 70</td><td rowspan="2">Example 71</td><td>Example</td><td>Example</td><td>Example</td>

<td> 72</td><td> 73</td><td> 74</td>

<td>Cyanocobalamin ***</td><td>6.3 mg</td><td>6.3 mg</td><td>6.3 mg</td><td>6.3 mg</td><td>6.3 mg</td>

<td>Photic acid</td><td>1.3 g</td><td>1.3 g</td><td>1.3 g</td><td>1.3 g</td><td>1.3g</td>

<td>Potassium iodide</td><td>204 mg</td><td>204 mg</td><td>204 mg</td><td>204 mg</td><td>204 mg</td>

<td>Beta-Alanine</td><td> 0</td><td> 0</td><td> 0</td><td> 1.0</td><td> 1.0</td>

* UTM / TM premix; ** ADEK Premix;

*** WSV Vitamin Premix

Examples 75-84

Examples 75-84 illustrate the concentrated liquid human milk fortifiers of the present disclosure, the ingredients of which are listed in the following table. To prepare the concentrated liquid human milk fortifiers, at least three separate suspensions (eg, the CHO-MIN suspension and the PIW suspension) are prepared and then mixed together. The modalities explained below that include pea protein hydrolyzates as the only protein source are the hypoallergenic modalities.

<td>Ingredients</td><td colspan="5">Amounts per 1000 Ib</td>

<td></td><td>Example 75</td><td>Example 76</td><td>Example 77</td><td>Example 78</td><td>Example 79</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

103

<td colspan="6">ΤΜΡΤ ^^</td><td rowspan="3"> 1</td>

<td>Ingredients</td><td colspan="5">Amounts per INDUSTRIAL</td>

<td rowspan="2"></td><td rowspan="2">Example 75</td><td rowspan="2">Example 76</td><td>Example</td><td>Example</td><td>Example</td>

<td> 77</td><td> 78</td><td> 79</td><td rowspan="18"></td>

<td>Protein hydrolyzate pea</td><td>104.6 Ib</td><td>99.4 Ib</td><td>109.8 Ib</td><td>94.1 Ib</td><td>115.1 Ib</td>

<td>Maltodextrin</td><td>93.0 Ib</td><td>93.0 Ib</td><td>93.0 Ib</td><td>93.0 Ib</td><td>93.0 Ib</td>

<td>MCT Oil</td><td>21.6 Ib</td><td>21.61b</td><td>21.6 Ib</td><td>21.6 Ib</td><td>21.6 Ib</td>

<td>Tricalcium phosphate ultramicronized</td><td>17.81b</td><td>17.8 Ib</td><td>17.8 Ib</td><td>17.8 Ib</td><td>17.8 Ib</td>

<td>Soy oil</td><td>12.91b</td><td>12.91b</td><td>12.9 Ib</td><td>12.91b</td><td>12.91b</td>

<td>Cornstarch modified with OSA</td><td>12.01b</td><td>12.01b</td><td>12.0 Ib</td><td>12.0 Ib</td><td>12.01b</td>

<td>Coconut oil</td><td>7.91b</td><td>7.9 Ib</td><td>7.9 Ib</td><td>7.9 Ib</td><td>7.91b</td>

<td>Potassium citrate</td><td>7.0 Ib</td><td>7.0 Ib</td><td>7.0 Ib</td><td>7.0 Ib</td><td>7.01b</td>

<td>Ascorbic acid</td><td>5.4 Ib</td><td>5.4 Ib</td><td>5.4 Ib</td><td>5.4 Ib</td><td>5.4 Ib</td>

<td>Magnesium chloride</td><td>3.91b</td><td>3.9 Ib</td><td>3.9 Ib</td><td>3.9 Ib</td><td>3.91b</td>

<td>ARA</td><td>3.4 Ib</td><td>3.4 Ib</td><td>3.4 Ib</td><td>3.4 Ib</td><td>3.4 Ib</td>

<td>L-leucine</td><td>2.81b</td><td>2.8 Ib</td><td>2.8 Ib</td><td>2.8 Ib</td><td>2.8 Ib</td>

<td>DHA</td><td>2.6 Ib</td><td>2.6 Ib</td><td>2.6 Ib</td><td>2.6 Ib</td><td>2.6 Ib</td>

<td>Potassium chloride</td><td>1.61b</td><td>1.6 Ib</td><td>1.6 Ib</td><td>1.6 Ib</td><td>1.6 Ib</td>

<td>L-tyrosine</td><td>1.4 Ib</td><td>1.4 Ib</td><td>1.4 Ib</td><td>1.4 Ib</td><td>1.4 Ib</td>

<td>Distilled Monoglycerides</td><td>1.01b</td><td>1.01b</td><td>1.0 Ib</td><td>1.0 Ib</td><td>1.0 Ib</td>

<td>Premix nucleotide / choline</td><td>399.0 g</td><td>399.0 g</td><td>399.0 g</td><td>399.0 g</td><td>399.0 g</td>

104

<td>Ingredients</td><td colspan="5">Quantities per box 4Mb Ib 1 INSTITUTO MEXICAN »&gt;: OT THE PROPERTY í</td>

<td rowspan="2"></td><td rowspan="2">Example 75</td><td rowspan="2">Example 76</td><td>Example</td><td>Example</td><td>Example'</td>

<td> 77</td><td> 7«</td><td> --</td>

<td>M-Inositol</td><td>296.0 g</td><td>296.0 g</td><td>296.0 g</td><td>296.0 g</td><td>296.0 g</td>

<td>Sodium chloride</td><td>246.3 g</td><td>246.3 g</td><td>246.3 g</td><td>246.3 g</td><td>246.3 g</td>

<td>L-carnitine</td><td>147.0 g</td><td>147.0 g</td><td>147.0 g</td><td>147.0 g</td><td>147.0 g</td>

<td>Zinc sulfate</td><td>127.0 g</td><td>127.0 g</td><td>127.0 g</td><td>127.0 g</td><td>127.0 g</td>

<td>Niacinamide</td><td>124.0 g</td><td>124.0 g</td><td>124.0 g</td><td>124.0 g</td><td>124.0 g</td>

<td>Di-alpha- acetate tocopheryl</td><td>114.0 g</td><td>114.0g</td><td>114.0g</td><td>114.0g</td><td>114.0g</td>

<td>Gellan gum</td><td>90.7 g</td><td>90.7 g</td><td>90.7 g</td><td>90.7 g</td><td>90.7 g</td>

<td>Ferrous sulfate</td><td>56.0 g</td><td>56.0 g</td><td>56.0 g</td><td>56.0 g</td><td>56.0 g</td>

<td>Choline Chloride</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td>

<td>Calcium Pantothenate</td><td>39.3 g</td><td>39.3 g</td><td>39.3 g</td><td>39.3 g</td><td>39.3 g</td>

<td>Vitamin A Palmitate</td><td>24.0 g</td><td>24.0 g</td><td>24.0 g</td><td>24.0 g</td><td>24.0 g</td>

<td>Riboflavin</td><td>9.4 g</td><td>9.4 g</td><td>9.4 g</td><td>9.4 g</td><td>9.4 g</td>

<td>Vitamin D3</td><td>8.6 g</td><td>8.6 g</td><td>8.6 g</td><td>8.6 g</td><td>8.6 g</td>

<td>Copper sulphate</td><td>8.2 g</td><td>8.2 g</td><td>8.2 g</td><td>8.2 g</td><td>8.2 g</td>

<td>Pyridoxine hydrochloride</td><td>6.3 g</td><td>6.3 g</td><td>6.3 g</td><td>6.3 g</td><td>6.3 g</td>

<td>Thiamine hydrochloride</td><td>6.1 g</td><td>6.1 g</td><td>6.1 g</td><td>6.1 g</td><td>6.1 g</td>

<td>Lutein</td><td>2.5 g</td><td>2.5 g</td><td>2.5 g</td><td>2.5 g</td><td>2.5 g</td>

<td>Beta-carotene</td><td>1.7 g</td><td>1-7 g</td><td>17g</td><td>1 7 g</td><td>1.7 g</td>

<td>Folic acid</td><td>0.88 g</td><td>0.88 g</td><td>0.88 g</td><td>0.88 g</td><td>0.88 g</td>

<td>Biotin</td><td>0.74 g</td><td>0.74 g</td><td>0.74 g</td><td>0.74 g</td><td>0.74 g</td>

105

IMPIOUS*

-------------- . '

<td>Ingredients</td><td colspan="5">- ---------------- iNSTmm ^ Amounts for each</td>

<td rowspan="2"></td><td rowspan="2">Example 75</td><td rowspan="2">Example 76</td><td>Example.</td><td>Example</td><td>Example</td>

<td> 77</td><td> 78</td><td> 79</td>

<td>Manganese sulphate</td><td>06.8 g</td><td>06.8 g</td><td>06.8 g</td><td>06.8 g</td><td>06.8 g</td>

<td>Phylloquinone</td><td>0.31 g</td><td>0.31 g</td><td>0.31 g</td><td>0.31 g</td><td>0.31 g</td>

<td>Sodium selenate</td><td>0.06 g</td><td>0.06 g</td><td>0.06 g</td><td>0.06 g</td><td>0.06 g</td>

<td>Cyanocobalamin</td><td>0.02 g</td><td>0.02 g</td><td>0.02 g</td><td>0.02 g</td><td>0.02 g</td>

<td>Ingredients</td><td colspan="5">Amounts per 1000 Ib</td>

<td></td><td>Example 80</td><td>Example 81</td><td>Example 82</td><td>Example 83</td><td>Example 84</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Protein hydrolyzate pea</td><td>52.3 Ib</td><td>62.76 Ib</td><td>73.22 Ib.</td><td>83.68 Ib</td><td>94.14 Ib</td>

<td>Calcium hydrolyzate</td><td>52.3 Ib</td><td>41,841b</td><td>31.38 Ib</td><td>20.92 Ib</td><td>10.46 Ib</td>

<td>Maltodextrin</td><td>93.0 Ib</td><td>93.0 Ib</td><td>93.0 Ib</td><td>93.0 Ib</td><td>93.0 Ib</td>

<td>MCT Oil</td><td>21.61b</td><td>21.61b</td><td>21.6 Ib</td><td>21.6 Ib</td><td>21.61b</td>

<td>Tricalcium phosphate ultramicronized</td><td>17.81b</td><td>17.81b</td><td>17.8 Ib</td><td>17.81b</td><td>17.81b</td>

<td>Soy oil</td><td>12.91b</td><td>12.91b</td><td>12.91b</td><td>12.9 Ib</td><td>12.91b</td>

<td>Cornstarch modified with OSA</td><td>12.01b</td><td>12.01b</td><td>12.01b</td><td>12.01b</td><td>12.01b</td>

<td>Coconut oil</td><td>7.91b</td><td>7.9 Ib</td><td>7.9 Ib</td><td>7.9 Ib</td><td>7.9 Ib</td>

<td>Potassium citrate</td><td>7.01b</td><td>7.0 Ib</td><td>7.0 Ib</td><td>7.01b</td><td>7.0 Ib</td>

106

WICKED

<td>Ingredients</td><td colspan="5">Amounts per cadft49001bacA ^ D £ THE PROPERTY * INDUSTRIAL</td>

<td rowspan="2"></td><td rowspan="2">Example 80</td><td rowspan="2">Example 81</td><td>Example</td><td>Example</td><td>Example</td>

<td> 82</td><td> 83</td><td> 84</td>

<td>Ascorbic acid</td><td>5.4 Ib</td><td>5.4 Ib</td><td>5.4 Ib</td><td>5.4 Ib</td><td>5.4 Ib</td>

<td>Magnesium chloride</td><td>3.9 Ib</td><td>3.9 Ib</td><td>3.9 Ib</td><td>3.9 Ib</td><td>3.9 Ib</td>

<td>ARA</td><td>3.4 Ib</td><td>3.4 Ib</td><td>3.4 Ib</td><td>3.4 Ib</td><td>3.4 Ib</td>

<td>L-leucine</td><td>2.81b</td><td>2.8 Ib</td><td>2.8 Ib</td><td>2.8 Ib</td><td>2.8 Ib</td>

<td>DHA</td><td>2.6 Ib</td><td>2.6 Ib</td><td>2.6 Ib</td><td>2.6 Ib</td><td>2.6 Ib</td>

<td>Potassium chloride</td><td>1.61b</td><td>1.61b</td><td>1.6 Ib</td><td>1.6 Ib</td><td>1.61b</td>

<td>L-tyrosine</td><td>1.4 Ib</td><td>1.4 Ib</td><td>1.4 Ib</td><td>1.4 Ib</td><td>1.4 Ib</td>

<td>Distilled Monoglycerides</td><td>1.01b</td><td>1.01b</td><td>1.01b</td><td>1.01b</td><td>1.01b</td>

<td>Premix nucleotide / choline</td><td>399.0 g</td><td>399.0 g</td><td>399.0 g</td><td>399.0 g</td><td>399.0 g</td>

<td>M-Inositol</td><td>296.0 g</td><td>296.0 g</td><td>296.0 g</td><td>296.0 g</td><td>296.0 g</td>

<td>Sodium chloride</td><td>246.3 g</td><td>246.3 g</td><td>246.3 g</td><td>246.3 g</td><td>246.3 g</td>

<td>L-carnitine</td><td>147.0 g</td><td>147.0 g</td><td>147.0 g</td><td>147.0 g</td><td>147.0 g</td>

<td>Zinc sulfate</td><td>127.0 g</td><td>127.0 g</td><td>127.0 g</td><td>127.0 g</td><td>127.0 g</td>

<td>Niacinamide</td><td>124.0 g</td><td>124.0 g</td><td>124.0 g</td><td>124.0 g</td><td>124.0 g</td>

<td>Di-alphatocopheryl acetate</td><td>114.0g</td><td>114.Og</td><td>114.0g</td><td>114.0g</td><td>114.Og</td>

<td>Gellan gum</td><td>90.7 g</td><td>90.7 g</td><td>90.7 g</td><td>90.7 g</td><td>90.7 g</td>

<td>Ferrous sulfate</td><td>56.0 g</td><td>56.0 g</td><td>56.0 g</td><td>56.0 g</td><td>56.0 g</td>

<td>Choline Chloride</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td><td>48.0 g</td>

<td>Calcium pantothenate</td><td>39.3 g</td><td>39.3 g</td><td>39.3 g</td><td>39.3 g</td><td>39.3 g</td>

107

IMPI

<td>Ingredients</td><td colspan="5">------------------------------------------ INSTITUTO MEXICANO— Amounts for each</td>

<td rowspan="2"></td><td rowspan="2">Example 80</td><td rowspan="2">Example 81</td><td>Example</td><td>Example</td><td>Example</td>

<td> 82</td><td> 83</td><td> 84</td>

<td>Vitamin A Palmitate</td><td>24.0 g</td><td>24.0 g</td><td>24.0 g</td><td>24.0 g</td><td>24.0 g</td>

<td>Riboflavin</td><td>9.4 g</td><td>9.4 g</td><td>9.4 g</td><td>9.4 g</td><td>9.4 g</td>

<td>Vitamin D3</td><td>8.6 g</td><td>8.6 g</td><td>8.6 g</td><td>8.6 g</td><td>8.6 g</td>

<td>Copper sulphate</td><td>8.2 g</td><td>8.2 g</td><td>8.2 g</td><td>8.2 g</td><td>8.2 g</td>

<td>Pyridoxine hydrochloride</td><td>6.3 g</td><td>6.3 g</td><td>6.3 g</td><td>6.3 g</td><td>6.3 g</td>

<td>Thiamine hydrochloride</td><td>6.1 g</td><td>6.1 g</td><td>6.1 g</td><td>6.1 g</td><td>6.1 g</td>

<td>Lutein</td><td>2.5 g</td><td>2.5 g</td><td>2.5 g</td><td>2.5 g</td><td>2.5 g</td>

<td>Beta-carotene</td><td>17 g</td><td>1.7 g</td><td>1.7 g</td><td>1-7 g</td><td>17g</td>

<td>Folic acid</td><td>0.88 g</td><td>0.88 g</td><td>0.88 g</td><td>0.88 g</td><td>0.88 g</td>

<td>Biotin</td><td>0.74 g</td><td>0.74 g</td><td>0.74 g</td><td>0.74 g</td><td>0.74 g</td>

<td>Manganese sulphate</td><td>06.8 g</td><td>06.8 g</td><td>06.8 g</td><td>06.8 g</td><td>06.8 g</td>

<td>Phylloquinone</td><td>0.31 g</td><td>0.31 g</td><td>0.31 g</td><td>0.31 g</td><td>0.31 g</td>

<td>Sodium selenate</td><td>0.06 g</td><td>0.06 g</td><td>0.06 g</td><td>0.06 g</td><td>0.06 g</td>

<td>Cyanocobalamin</td><td>0.02 g</td><td>0.02 g</td><td>0.02 g</td><td>0.02 g</td><td>0.02 g</td>

Examples 85-94

The following examples illustrate a human milk fortifying powder added to human milk that can be administered to an infant according to the methods of the present disclosure. The ingredients for the manufacture of 8.172 kg of powdered human milk fortifier are listed in the table below.

108

<td>Ingredients</td><td colspan="5">Amount to formulate 8 'INSTITWTO MEXICANO ·,' OF THE PROPERTY&gt;</td>

<td rowspan="2"></td><td rowspan="2">Example 85</td><td rowspan="2">Example 86</td><td>Example</td><td>Example</td><td>Example</td>

<td> 87</td><td> 88</td><td> 89</td>

<td>Ingredient water</td><td>16,205 I</td><td>16,205 I</td><td> 16,2051</td><td>16,205 I</td><td>16,205 I</td>

<td>Corn Syrup Solids</td><td>1603 kg</td><td>1603 kg</td><td>1603 kg</td><td>1603 kg</td><td>1603 kg</td>

<td>Magnesium chloride</td><td>96.2 kg</td><td>96.2 kg</td><td>96.2 kg</td><td>96.2 kg</td><td>96.2 kg</td>

<td>Potassium citrate</td><td>223.8 kg</td><td>223.8 kg</td><td>223.8 kg</td><td>223.8 kg</td><td>223.8 kg</td>

<td>Sodium citrate</td><td>6.6 kg</td><td>6.6 kg</td><td>6.6 kg</td><td>6.6 kg</td><td>6.6 kg</td>

<td>Sodium chloride</td><td>15.4 kg</td><td>15.4 kg</td><td>15.4 kg</td><td>15.4 kg</td><td>15.4 kg</td>

<td>TCM oil</td><td>801 kg</td><td>801 kg</td><td>801 kg</td><td>801 kg</td><td>801 kg</td>

<td>Lecithin</td><td>16.6 kg</td><td>16.6 kg</td><td>16.6 kg</td><td>16.6 kg</td><td>16.6 kg</td>

<td>Vitamin A</td><td>2.36 kg</td><td>2.36 kg</td><td>2.36 kg</td><td>2.36 kg</td><td>2.36 kg</td>

<td>Vitamin D</td><td>359.3 g</td><td>359.3 g</td><td>359.3 g</td><td>359.3 g</td><td>359.3 g</td>

<td>Vitamin K</td><td>27.5 g</td><td>27.5 g</td><td>27.5 g</td><td>27.5 g</td><td>27.5 g</td>

<td>Tocopherol Acetate</td><td>15.8 kg</td><td>15.8 kg</td><td>15.8 kg</td><td>15.8 kg</td><td>15.8 kg</td>

<td>Calcium carbonate</td><td>33.1 kg</td><td>33.1 kg</td><td>33.1 kg</td><td>33.1 kg</td><td>33.1 kg</td>

<td>Tricalcium phosphate</td><td>646 kg</td><td>646 kg</td><td>646 kg</td><td>646 kg</td><td>646 kg</td>

<td>Protein hydrolyzate pea</td><td>2696.5 kg</td><td>2561.7 kg</td><td>2831.3 kg</td><td>2426.9 kg</td><td>2966.2 kg</td>

<td>Potassium citrate</td><td>257.2 g</td><td>257.2 g</td><td>257.2 g</td><td>257.2 g</td><td>257.2 g</td>

<td>Ferrous sulfate</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td>

<td>Zinc sulfate</td><td>11.1 kg</td><td>11.1 kg</td><td>11.1 kg</td><td>11.1 kg</td><td>11.1 kg</td>

<td>Copper sulphate</td><td>1.84 kg</td><td>1.84 kg</td><td>1.84 kg</td><td>1.84 kg</td><td>1.84 kg</td>

<td>Manganese sulphate</td><td>0.320 kg</td><td>0.320 kg</td><td>0.320 kg</td><td>0.320 kg</td><td>0.320 kg</td>

109

<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td>

<td></td><td>Ingredients</td><td colspan="3">Amount to formulate 8.17 #</td><td>of the industrial prohedae</td><td></td><td></td>

<td></td><td></td><td>Example</td><td>Example</td><td>Example</td><td>Example</td><td>Example ,</td><td></td>

<td></td><td></td><td> 85</td><td> 86</td><td> 87</td><td> 88</td><td> 89</td><td></td>

<td></td><td>Sodium selenate</td><td>0.001 kg</td><td>0.001 kg</td><td>0.001 kg</td><td>0.001 kg</td><td>0.001 kg</td><td></td>

<td> 5</td><td>Niacinamide</td><td>0.98 kg</td><td>0.98 kg</td><td>0.98 kg</td><td>0.98 kg</td><td>0.98 kg</td><td></td>

<td></td><td>Riboflavin</td><td>1.14 kg</td><td>1.14 kg</td><td>1.14 kg</td><td>1.14 kg</td><td>1.14 kg</td><td></td>

<td></td><td>Calcium pantothenate</td><td>4.08 kg</td><td>4.08 kg</td><td>4.08 kg</td><td>4.08 kg</td><td>4.08 kg</td><td></td>

<td></td><td>Pyridoxine hydrochloride</td><td>0.655 kg</td><td>0.655 kg</td><td>0.655 kg</td><td>0.655 kg</td><td>0.655 kg</td><td></td>

<td></td><td>m-inositol</td><td>9.55 kg</td><td>9.55 kg</td><td>9.55 kg</td><td>9.55 kg</td><td>9.55 kg</td><td></td>

<td> 10</td><td>Biotin</td><td>0.0727 kg</td><td>0.0727 kg</td><td>0.0727 kg</td><td>0.0727 kg</td><td>0.0727 kg</td><td></td>

<td></td><td>Photic acid</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td><td></td>

<td></td><td>Cyanocobalamin</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td><td></td>

<td></td><td>Ascorbic acid</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td><td></td>

<td> 15</td><td>Ingredients</td><td colspan="5">Amount to formulate 8,172 kg of fortifier</td>

<td></td><td></td><td>Example 90</td><td>Example 91</td><td>Example 92</td><td>Example 93</td><td>Example 94</td>

<td></td><td>Ingredient water</td><td>16,205 I</td><td>16,205 I</td><td> 16,2051</td><td>16,205 I</td><td>16,205 I</td>

<td></td><td>Corn Syrup Solids</td><td>1603 kg</td><td>1603 kg</td><td>1603 kg</td><td>1603 kg</td><td>1603 kg</td>

<td> 20</td><td>Magnesium chloride</td><td>96.2 kg</td><td>96.2 kg</td><td>96.2 kg</td><td>96.2 kg</td><td>96.2 kg</td>

<td></td><td>Potassium citrate</td><td>223.8 kg</td><td>223.8 kg</td><td>223.8 kg</td><td>223.8 kg</td><td>223.8 kg</td>

<td></td><td>Sodium citrate</td><td>6.6 kg</td><td>6.6 kg</td><td>6.6 kg</td><td>6.6 kg</td><td>6.6 kg</td>

<td></td><td>Sodium chloride</td><td>15.4 kg</td><td>15.4 kg</td><td>15.4 kg</td><td>15.4 kg</td><td>15.4 kg</td>

<td></td><td>TCM oil</td><td>801 kg</td><td>801 kg</td><td>801 kg</td><td>801 kg</td><td>801 kg</td>

110

<td>Ingredients</td><td colspan="5">Amount to formulate 8.47¿ ¥ &amp; (£ fáti ^ ínifé. INSTmiTu MtXICANO SAY THE PROPERTY Qua-TJX / A · '</td>

<td rowspan="2"></td><td rowspan="2">Example 90</td><td rowspan="2">Example 91</td><td>Example</td><td>Example</td><td>Example</td>

<td> 92</td><td> 93</td><td> 94</td>

<td>Lecithin</td><td>16.6 kg</td><td>16.6 kg</td><td>16.6 kg</td><td>16.6 kg</td><td>16.6 kg</td>

<td>Vitamin A</td><td>2.36 kg</td><td>2.36 kg</td><td>2.36 kg</td><td>2.36 kg</td><td>2.36 kg</td>

<td>Vitamin D</td><td>359.3 g</td><td>359.3 g</td><td>359.3 g</td><td>359.3 g</td><td>359.3 g</td>

<td>Vitamin K</td><td>27.5 g</td><td>27.5 g</td><td>27.5 g</td><td>27.5 g</td><td>27.5 g</td>

<td>Tocopherol Acetate</td><td>15.8 kg</td><td>15.8 kg</td><td>15.8 kg</td><td>15.8 kg</td><td>15.8 kg</td>

<td>Calcium carbonate</td><td>33.1 kg</td><td>33.1 kg</td><td>33.1 kg</td><td>33.1 kg</td><td>33.1 kg</td>

<td>Tricalcium phosphate</td><td>646 kg</td><td>646 kg</td><td>646 kg</td><td>646 kg</td><td>646 kg</td>

<td>Pea protein hydrolyzate</td><td>1348.3 kg</td><td>1537.0 kg</td><td>1981.9 kg</td><td>1941.5 kg</td><td>2669.58 kg</td>

<td>Soy protein concentrate</td><td>753.0 kg</td><td>602.4 kg</td><td>451.8 kg</td><td>301.2 kg</td><td>150.6 kg</td>

<td>Nonfat Powdered Milk</td><td>1653.5 kg</td><td>1322.8 kg</td><td>992.1 kg</td><td>661.4 kg</td><td>3.31 kg</td>

<td>Potassium citrate</td><td>257.2 g</td><td>257.2 g</td><td>257.2 g</td><td>257.2 g</td><td>257.2 g</td>

<td>Ferrous sulfate</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td><td>3.7 kg</td>

<td>Zinc sulfate</td><td>11.1 kg</td><td>11.1 kg</td><td>11.1 kg</td><td>11.1 kg</td><td>11.1 kg</td>

<td>Copper sulphate</td><td>1.84 kg</td><td>1.84 kg</td><td>1.84 kg</td><td>1.84 kg</td><td>1.84 kg</td>

<td>Manganese sulphate</td><td>0.320 kg</td><td>0.320 kg</td><td>0.320 kg</td><td>0.320 kg</td><td>0.320 kg</td>

<td>Sodium selenate</td><td>0.001 kg</td><td>0.001 kg</td><td>0.001 kg</td><td>0.001 kg</td><td>0.001 kg</td>

<td>Niacinamide</td><td>0.98 kg</td><td>0.98 kg</td><td>0.98 kg</td><td>0.98 kg</td><td>0.98 kg</td>

<td>Riboflavin</td><td>1.14 kg</td><td>1.14 kg</td><td>1.14 kg</td><td>1.14 kg</td><td>1.14 kg</td>

<td>Calcium pantothenate</td><td>4.08 kg</td><td>4.08 kg</td><td>4.08 kg</td><td>4.08 kg</td><td>4.08 kg</td>

lll

<td>Ingredients</td><td colspan="5">Amount to formulate OF INDUSTRIAL PROPERTY</td>

<td rowspan="2"></td><td rowspan="2">Example 90</td><td rowspan="2">Example 91</td><td>Example</td><td>Example</td><td>Example</td>

<td> 92</td><td> 93</td><td> 94</td>

<td>Pyridoxine hydrochloride</td><td>0.655 kg</td><td>0.655 kg</td><td>0.655 kg</td><td>0.655 kg</td><td>0.655 kg</td>

<td>m-inositol</td><td>9.55 kg</td><td>9.55 kg</td><td>9.55 kg</td><td>9.55 kg</td><td>9.55 kg</td>

<td>Biotin</td><td>0.0727 kg</td><td>0.0727 kg</td><td>0.0727 kg</td><td>0.0727 kg</td><td>0.0727 kg</td>

<td>Folic acid</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td>

<td>Cyanocobalamin</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td>

<td>Ascorbic acid</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td>

Examples 95-1 04

In these examples, a gelled human milk fortifier is prepared in accordance with the present disclosure. The ingredients for the Gelled Human Milk Fortifier are shown in the table below.

Gelled Human Milk Fortifier is prepared by solubilizing and combining the ingredients in a homogeneous aqueous mixture that undergoes a suitable heat treatment to achieve long-term non-perishable ability.

To begin the manufacturing process, the ingredients that supply the macronutrients (carbohydrates, proteins, fats and minerals) are combined in multiple suspensions together and with water. This mixture undergoes an initial heat treatment and is then tested for proper nutrient levels.

112

<td>Ingredients</td><td colspan="5">Quantity to formulate 32, odbi &amp; r ^^ ortilic ^^ tsíΊ OF THE PROPERTY . industrial</td>

<td rowspan="2"></td><td rowspan="2">Example 95</td><td rowspan="2">Example 96</td><td>Example</td><td>Example</td><td>Example</td>

<td> 97</td><td> 98</td><td> 99</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Corn maltodextrin</td><td>1450 kg</td><td>1450 kg</td><td>1450 kg</td><td>1450 kg</td><td>1450 kg</td>

<td>Syrup solids corn</td><td>1388 kg</td><td>1388 kg</td><td>1388 kg</td><td>1388kg</td><td>1388kg</td>

<td>Chain triglycerides median</td><td>694 kg</td><td>694 kg</td><td>694 kg</td><td>694 kg</td><td>694 kg</td>

<td>Pea protein hydrolyzate</td><td>1944.4 kg</td><td>1847.18 kg</td><td>2041.6 kg</td><td>1750.0 kg</td><td>2138.8 kg</td>

<td>Calcium phosphate</td><td>271 kg</td><td>271 kg</td><td>271 kg</td><td>271 kg</td><td>271 kg</td>

<td>Ascorbic acid</td><td>152 kg</td><td>152 kg</td><td>152 kg</td><td>152 kg</td><td>152 kg</td>

<td>Magnesium chloride</td><td>38.0 kg</td><td>38.0 kg</td><td>38.0 kg</td><td>38.0 kg</td><td>38.0 kg</td>

<td>Potassium citrate</td><td>12.2 kg</td><td>12.2 kg</td><td>12.2 kg</td><td>12.2 kg</td><td>12.2 kg</td>

<td>Sodium chloride</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td>

<td>Soy lecithin</td><td>8.84 kg</td><td>8.84 kg</td><td>8.84 kg</td><td>8.84 kg</td><td>8.84 kg</td>

<td>M-Inositol</td><td>7.98 kg</td><td>7.98 kg</td><td>7.98 kg</td><td>7.98 kg</td><td>7.98 kg</td>

<td>Magnesium phosphate</td><td>5.55 kg</td><td>5.55 kg</td><td>5.55 kg</td><td>5.55 kg</td><td>5.55 kg</td>

<td>M. Alpina oil</td><td>5.35 kg</td><td>5.35 kg</td><td>5.35 kg</td><td>5.35 kg</td><td>5.35 kg</td>

<td>Niacinamide</td><td>4.35 kg</td><td>4.35 kg</td><td>4.35 kg</td><td>4.35 kg</td><td>4.35 kg</td>

<td>Di-alpha- acetate tocopherol</td><td>4.21 kg</td><td>4.21 kg</td><td>4.21 kg</td><td>4.21 kg</td><td>4.21 kg</td>

<td>Zinc sulfate</td><td>3.51 kg</td><td>3.51 kg</td><td>3.51 kg</td><td>3.51 kg</td><td>3.51 kg</td>

113

<td>Ingredients</td><td colspan="5">-----------------------------------Y* Amount to formulate 32, INDUSTRIAL</td>

<td rowspan="2"></td><td rowspan="2">Example 95</td><td rowspan="2">Example 96</td><td rowspan="2">Example 97</td><td>Example</td><td>Example</td>

<td> 98</td><td> 99</td>

<td>C. Cohnii oil</td><td>3.45 kg</td><td>3.45 kg</td><td>3.45 kg</td><td>3.45 kg</td><td>3.45 kg</td>

<td>Choline Chloride</td><td>2.90 kg</td><td>2.90 kg</td><td>2.90 kg</td><td>2.90 kg</td><td>2.90 kg</td>

<td>Calcium pantothenate</td><td>1.89 kg</td><td>1.89 kg</td><td>1.89 kg</td><td>1.89 kg</td><td>1.89 kg</td>

<td>Potassium Phosphate</td><td>1.62 kg</td><td>1.62 kg</td><td>1.62 kg</td><td>1.62 kg</td><td>1.62 kg</td>

<td>Ferrous sulfate</td><td>1.64 kg</td><td>1.64 kg</td><td>1.64 kg</td><td>1.64 kg</td><td>1.64 kg</td>

<td>Vitamin A Palmitate</td><td>900 g</td><td>900 g</td><td>900 g</td><td>900 g</td><td>900 g</td>

<td>Cupric sulfate</td><td>678 g</td><td>678 g</td><td>678 g</td><td>678 g</td><td>678 g</td>

<td>Riboflavin</td><td>572 g</td><td>572 g</td><td>572 g</td><td>572 g</td><td>572 g</td>

<td>Thiamine hydrochloride</td><td>373 g</td><td>373 g</td><td>373 g</td><td>373 g</td><td>373 g</td>

<td>Pyridoxine hydrochloride</td><td>232 g</td><td>232 g</td><td>232 g</td><td>232 g</td><td>232 g</td>

<td>Vitamin D3</td><td>152 g</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td>

<td>Folic acid</td><td>47.9 g</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td>

<td>Biotin</td><td>34.1 g</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td>

<td>Manganese sulphate</td><td>23.2 g</td><td>23.2 g</td><td>23.2 g</td><td>23.2 g</td><td>23.2 g</td>

<td>Phylloquinone</td><td>11.6 g</td><td>11.6 g</td><td>11.6 g</td><td>11.6 g</td><td>11.6 g</td>

<td>Cyanocobalamin</td><td>1.60 g</td><td>1.60 g</td><td>1.60 g</td><td>1.60 g</td><td>1.60 g</td>

<td>Sodium selenate</td><td>0.798 g</td><td>0.798 g</td><td>0.798 g</td><td>0.798 g</td><td>0.798 g</td>

<td>Calcium carbonate</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

114

IMPI

<td>Ingredients</td><td colspan="5">Amount to formulate INDUSTRIAL</td><td></td>

<td></td><td>Example</td><td>Example</td><td>Example</td><td>Example</td><td>Example</td><td></td>

<td></td><td> 95</td><td> 96</td><td> 97</td><td> 98</td><td> 99</td><td></td>

<td>Sodium citrate</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td></td>

<td>Potassium hydroxide</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td></td>

<td>Ingredients</td><td colspan="5">Amount to formulate 32,000 Ib of fortifier</td>

<td></td><td>Example 100</td><td>Example 101</td><td>Example 102</td><td>Example 103</td><td>Example 104</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

<td>Skim milk condensed</td><td>2625 kg</td><td>2100 kg</td><td>1575 kg</td><td>1050 kg</td><td>525 kg</td>

<td>Milk solids without grease</td><td>682.5 kg</td><td>546 kg</td><td>409.5 kg</td><td>273 kg</td><td>136.5 kg</td>

<td>Corn maltodextrin</td><td>1450 kg</td><td>1450 kg</td><td>1450 kg</td><td>1450 kg</td><td>1450 kg</td>

<td>Corn Syrup Solids</td><td>1388 kg</td><td>1388kg</td><td>1388 kg</td><td>1388 kg</td><td>1388kg</td>

<td>Chain triglycerides median</td><td>694 kg</td><td>694 kg</td><td>694 kg</td><td>694 kg</td><td>694 kg</td>

115

IMPIOUS

<td>Ingredients</td><td colspan="5">Amount to formulate</td>

<td rowspan="2"></td><td rowspan="2">Example 100</td><td rowspan="2">Example 101</td><td>Example ^</td><td>Example</td><td>Example</td>

<td> 102</td><td> 103</td><td> 104</td>

<td>Protein hydrolyzate pea</td><td>972.2 kg</td><td>1166.6 kg</td><td>1361.1 kg</td><td>1555.5 kg</td><td>1750.0 kg</td>

<td>Concentrate of whey protein</td><td>317 kg</td><td>253.6 kg</td><td>190.2 kg</td><td>126.8 kg</td><td>63.4 kg</td>

<td>Calcium phosphate</td><td>271 kg</td><td>271 kg</td><td>271 kg</td><td>271 kg</td><td>271 kg</td>

<td>Ascorbic acid</td><td>152 kg</td><td>152 kg</td><td>152 kg</td><td>152 kg</td><td>152 kg</td>

<td>Magnesium chloride</td><td>38.0 kg</td><td>38.0 kg</td><td>38.0 kg</td><td>38.0 kg</td><td>38.0 kg</td>

<td>Potassium citrate</td><td>12.2 kg</td><td>12.2 kg</td><td>12.2 kg</td><td>12.2 kg</td><td>12.2 kg</td>

<td>Sodium chloride</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td><td>12.1 kg</td>

<td>Soy lecithin</td><td>8.84 kg</td><td>8.84 kg</td><td>8.84 kg</td><td>8.84 kg</td><td>8.84 kg</td>

<td>M-Inositol</td><td>7.98 kg</td><td>7.98 kg</td><td>7.98 kg</td><td>7.98 kg</td><td>7.98 kg</td>

<td>Magnesium phosphate</td><td>5.55 kg</td><td>5.55 kg</td><td>5.55 kg</td><td>5.55 kg</td><td>5.55 kg</td>

<td>M. Alpina oil</td><td>5.35 kg</td><td>5.35 kg</td><td>5.35 kg</td><td>5.35 kg</td><td>5.35 kg</td>

<td>Niacinamide</td><td>4.35 kg</td><td>4.35 kg</td><td>4.35 kg</td><td>4.35 kg</td><td>4.35 kg</td>

<td>Di-alpha- acetate tocopherol</td><td>4.21 kg</td><td>4.21 kg</td><td>4.21 kg</td><td>4.21 kg</td><td>4.21 kg</td>

<td>Zinc sulfate</td><td>3.51 kg</td><td>3.51 kg</td><td>3.51 kg</td><td>3.51 kg</td><td>3.51 kg</td>

<td>C. Cohnii oil</td><td>3.45 kg</td><td>3.45 kg</td><td>3.45 kg</td><td>3.45 kg</td><td>3.45 kg</td>

<td>Choline Chloride</td><td>2.90 kg</td><td>2.90 kg</td><td>2.90 kg</td><td>2.90 kg</td><td>2.90 kg</td>

<td>Calcium pantothenate</td><td>1.89 kg</td><td>1.89 kg</td><td>1.89 kg</td><td>1.89 kg</td><td>1.89 kg</td>

<td>Potassium Phosphate</td><td>1.62 kg</td><td>1.62 kg</td><td>1.62 kg</td><td>1.62 kg</td><td>1.62 kg</td>

116

WICKED

Ρ ”ΤΠί? ΘΜ« _ιθΛ} &amp; tete XY

<td>Ingredients</td><td colspan="5">Quantity to formulate 32,000 ®</td>

<td></td><td>Example 100</td><td>Example 101</td><td>Example 102</td><td>-Example 103</td><td>Example 104</td>

<td>Ferrous sulfate</td><td>1.64 kg</td><td>1.64 kg</td><td>1.64 kg</td><td>1.64 kg</td><td>1.64 kg</td>

<td>Vitamin A Palmitate</td><td>900 g</td><td>900 g</td><td>900 g</td><td>900 g</td><td>900 g</td>

<td>Cupric sulfate</td><td>678 g</td><td>678 g</td><td>678 g</td><td>678 g</td><td>678 g</td>

<td>Riboflavin</td><td>572 g</td><td>572 g</td><td>572 g</td><td>572 g</td><td>572 g</td>

<td>Thiamine hydrochloride</td><td>373 g</td><td>373 g</td><td>373 g</td><td>373 g</td><td>373 g</td>

<td>Pyridoxine hydrochloride</td><td>232 g</td><td>232 g</td><td>232 g</td><td>232 g</td><td>232 g</td>

<td>Vitamin D3</td><td>152 g</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td><td>0.0775 kg</td>

<td>Folic acid</td><td>47.9 g</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td><td>0.0016 kg</td>

<td>Biotin</td><td>34.1 g</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td><td>153.5 kg</td>

<td>Manganese sulphate</td><td>23.2 g</td><td>23.2 g</td><td>23.2 g</td><td>23.2 g</td><td>23.2 g</td>

<td>Phylloquinone</td><td>11-6 g</td><td>11-6 g</td><td>11.6 g</td><td>11.6g</td><td>11.6g</td>

<td>Cyanocobalamin</td><td>1.60 g</td><td>1.60 g</td><td>1.60 g</td><td>1.60 g</td><td>1.60 g</td>

<td>Sodium selenate</td><td>0.798 g</td><td>0.798 g</td><td>0.798 g</td><td>0.798 g</td><td>0.798 g</td>

<td>Calcium carbonate</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

<td>Sodium citrate</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

<td>Potassium hydroxide</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td><td>as necessary</td>

117

IMPI -. . a ne aaa INSTITUTO MEXICANO —XÍP

EI m pIOS 1 05-11 4 nor the property ¹ industrial

Examples 105-114 illustrate the nutritional powders of the present disclosure, the ingredients of which are listed in the following table. These products will be prepared through spray drying methods in separate batches, reconstituted with water prior to use at the desired target ingredient concentrations. All ingredient quantities are listed as kg per 1000 kg batch of product, unless otherwise specified.

<td>Ingredients</td><td colspan="5">Amounts (kg) per 1000 kg</td>

<td></td><td>Example 105</td><td>Example 106</td><td>Example 107</td><td>Example 108</td><td>Example 109</td>

<td>Maltrin</td><td> 436.7</td><td> 436.7</td><td> 436.7</td><td> 436.7</td><td> 436.7</td>

<td>Sucrose</td><td> 145.5</td><td> 145.5</td><td> 145.5</td><td> 145.5</td><td> 145.5</td>

<td>Pea protein hydrolyzate</td><td> 186.8</td><td> 177.5</td><td> 196.14</td><td> 168.12</td><td> 205.5</td>

<td>Conjugated linoleic acid</td><td> 67.0</td><td> 63.65</td><td> 70.35</td><td> 60.3</td><td> 73.7</td>

<td>FOS powder</td><td> 33.6</td><td> 33.6</td><td> 33.6</td><td> 33.6</td><td> 33.6</td>

<td>High oleic safflower oil</td><td> 33.1</td><td> 33.1</td><td> 33.1</td><td> 33.1</td><td> 33.1</td>

<td>Calcium P-hydroxy-β-methylbutarate</td><td> 31.6</td><td> 31.6</td><td> 31.6</td><td> 31.6</td><td> 31.6</td>

118

<td>Ingredients</td><td colspan="5">Quantities (kg) OF INDUSTRIAL PROPERTY</td>

<td rowspan="2"></td><td rowspan="2">Example 105</td><td rowspan="2">Example 106</td><td>Example</td><td>Example</td><td>Example</td>

<td> 107</td><td> 108</td><td> 109</td>

<td>Canola oil</td><td> 28.3</td><td> 28.3</td><td> 28.3</td><td> 28.3</td><td> 28.3</td>

<td>Soy oil</td><td> 13.3</td><td> 13.3</td><td> 13.3</td><td> 13.3</td><td> 13.3</td>

<td>Potassium citrate</td><td> 10.3</td><td> 10.3</td><td> 10.3</td><td> 10.3</td><td> 10.3</td>

<td>Sodium citrate</td><td> 5.8</td><td> 5.8</td><td> 5.8</td><td> 5.8</td><td> 5.8</td>

<td>Potassium chloride</td><td> 5.2</td><td> 5.2</td><td> 5.2</td><td> 5.2</td><td> 5.2</td>

<td>Magnesium chloride</td><td> 4.7</td><td> 4.7</td><td> 4.7</td><td> 4.7</td><td> 4.7</td>

<td>Potassium hydroxide</td><td> 3.1</td><td> 3.1</td><td> 3.1</td><td> 3.1</td><td> 3.1</td>

<td>Dibasic sodium phosphate dihydrate</td><td> 3.0</td><td> 3.0</td><td> 3.0</td><td> 3.0</td><td> 3.0</td>

<td>Sodium chloride</td><td> 2.5</td><td> 2.5</td><td> 2.5</td><td> 2.5</td><td> 2.5</td>

<td>Choline Chloride</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td>

<td>Natural and artificial vanilla flavoring</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td>

<td>Monobasic sodium phosphate monohydrate</td><td> 1.6</td><td> 1.6</td><td> 1.6</td><td> 1.6</td><td> 1.6</td>

<td>Monobasic Potassium Phosphate Trihydrate</td><td> 1.1</td><td> 1.1</td><td> 1.1</td><td> 1.1</td><td> 1.1</td>

119

<td colspan="6">IMPI Mexican institute '.. yes</td><td> 1</td>

<td>Ingredients</td><td colspan="5">Amounts (kg) for each</td><td rowspan="2"></td>

<td rowspan="2"></td><td rowspan="2">Example 105</td><td rowspan="2">Example 106</td><td></td><td></td><td></td>

<td> 107</td><td> 108</td><td> 109</td><td rowspan="12"></td>

<td>Artificial cotton candy flavor</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td>

<td>Vitamin premix</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td>

<td>Ascorbyl plamitate</td><td> 0.2437</td><td> 0.2437</td><td> 0.2437</td><td> 0.2437</td><td> 0.2437</td>

<td>Ascorbic acid</td><td> 0.2400</td><td> 0.2400</td><td> 0.2400</td><td> 0.2400</td><td> 0.2400</td>

<td>Food Grade Antioxidant Tocopherol-2</td><td> 0.1161</td><td> 0.1161</td><td> 0.1161</td><td> 0.1161</td><td> 0.1161</td>

<td>Ferrous sulfate</td><td> 0.0929</td><td> 0.0929</td><td> 0.0929</td><td> 0.0929</td><td> 0.0929</td>

<td>Vitamin premix</td><td> 0.065</td><td> 0.065</td><td> 0.065</td><td> 0.065</td><td> 0.065</td>

<td>Zinc sulfate monohydrate</td><td> 0.057</td><td> 0.057</td><td> 0.057</td><td> 0.057</td><td> 0.057</td>

<td>Manganese sulphate</td><td> 0.0454</td><td> 0.0454</td><td> 0.0454</td><td> 0.0454</td><td> 0.0454</td>

<td>Copper sulfate with mineral mixture</td><td> 0.0353</td><td> 0.0353</td><td> 0.0353</td><td> 0.0353</td><td> 0.0353</td>

<td>30% beta carotene</td><td> 0.0056</td><td> 0.0056</td><td> 0.0056</td><td> 0.0056</td><td> 0.0056</td>

120

IMPI ^

<td>Ingredients</td><td colspan="5">Amounts (kg) per INDUSTRIAL</td>

<td></td><td>Example 105</td><td>Example 106</td><td>Example 107</td><td>Example 108</td><td>Example 109</td>

<td>Chromium Chloride</td><td> 0.0013</td><td> 0.0013</td><td> 0.0013</td><td> 0.0013</td><td> 0.0013</td>

<td>Sodium molybdate</td><td> 0.0012</td><td> 0.0012</td><td> 0.0012</td><td> 0.0012</td><td> 0.0012</td>

<td>Potassium iodide</td><td> 0.001</td><td> 0.001</td><td> 0.001</td><td> 0.001</td><td> 0.001</td>

<td>Sodium selenite</td><td> 0.0004008</td><td> 0.0004008</td><td> 0.0004008</td><td> 0.0004008</td><td> 0.0004008</td>

<td>Citric acid</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Potassium hydroxide</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Dry magnesium sulfate</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Ultramicronized Tricalcium Phosphate</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Ascorbic acid</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Water</td><td>cs</td><td>cs</td><td>cs</td><td>cs</td><td>cs</td>

121

<td>Ingredients</td><td colspan="5">Amounts (kg) per INDUSTRIAL</td>

<td rowspan="2"></td><td rowspan="2">Example 110</td><td rowspan="2">Example 111</td><td>Example</td><td>Example</td><td>Example</td>

<td> 112</td><td> 113</td><td> 114</td>

<td>Maltrin</td><td> 436.7</td><td> 436.7</td><td> 436.7</td><td> 436.7</td><td> 436.7</td>

<td>Sucrose</td><td> 145.5</td><td> 145.5</td><td> 145.5</td><td> 145.5</td><td> 145.5</td>

<td>Calcium caseinate</td><td> 64.6</td><td> 51.6</td><td> 38.7</td><td> 25.8</td><td> 12.91</td>

<td>Pea protein hydrolyzate</td><td> 93.4</td><td> 112.1</td><td> 130.8</td><td> 149.4</td><td> 168.1</td>

<td>Conjugated linoleic acid</td><td> 67.0</td><td> 63.65</td><td> 70.35</td><td> 60.3</td><td> 73.7</td>

<td>Soy protein isolate</td><td> 28.8</td><td> 23.1</td><td> 17.3</td><td> 11.5</td><td> 5.77</td>

<td>FOS powder</td><td> 33.6</td><td> 33.6</td><td> 33.6</td><td> 33.6</td><td> 33.6</td>

<td>High oleic safflower oil</td><td> 33.1</td><td> 33.1</td><td> 33.1</td><td> 33.1</td><td> 33.1</td>

<td>Calcium P-hydroxy-Pmethylbutarate</td><td> 31.6</td><td> 31.6</td><td> 31.6</td><td> 31.6</td><td> 31.6</td>

<td>Canola oil</td><td> 28.3</td><td> 28.3</td><td> 28.3</td><td> 28.3</td><td> 28.3</td>

<td>Soy oil</td><td> 13.3</td><td> 13.3</td><td> 13.3</td><td> 13.3</td><td> 13.3</td>

<td>Potassium citrate</td><td> 10.3</td><td> 10.3</td><td> 10.3</td><td> 10.3</td><td> 10.3</td>

<td>Sodium citrate</td><td> 5.8</td><td> 5.8</td><td> 5.8</td><td> 5.8</td><td> 5.8</td>

<td>Potassium chloride</td><td> 5.2</td><td> 5.2</td><td> 5.2</td><td> 5.2</td><td> 5.2</td>

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WICKED

<td>Ingredients</td><td colspan="5">Amounts (kg) per industrial</td>

<td rowspan="2"></td><td rowspan="2">Example 110</td><td rowspan="2">Example 111</td><td>Example</td><td>Example</td><td>Example</td>

<td> 112</td><td> 113</td><td> 114</td>

<td>Magnesium chloride</td><td> 4.7</td><td> 4.7</td><td> 4.7</td><td> 4.7</td><td> 4.7</td>

<td>Potassium hydroxide</td><td> 3.1</td><td> 3.1</td><td> 3.1</td><td> 3.1</td><td> 3.1</td>

<td>Dibasic sodium phosphate dihydrate</td><td> 3.0</td><td> 3.0</td><td> 3.0</td><td> 3.0</td><td> 3.0</td>

<td>Sodium chloride</td><td> 2.5</td><td> 2.5</td><td> 2.5</td><td> 2.5</td><td> 2.5</td>

<td>Choline Chloride</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td>

<td>Natural and artificial vanilla flavoring</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td><td> 1.8</td>

<td>Monobasic sodium phosphate monohydrate</td><td> 1.6</td><td> 1.6</td><td> 1.6</td><td> 1.6</td><td> 1.6</td>

<td>Monobasic Potassium Phosphate Trihydrate</td><td> 1.1</td><td> 1.1</td><td> 1.1</td><td> 1.1</td><td> 1.1</td>

<td>Artificial cotton candy flavor</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td>

<td>Vitamin premix</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td><td> 1.0</td>

<td>Ascorbyl plamitate</td><td> 0.2437</td><td> 0.2437</td><td> 0.2437</td><td> 0.2437</td><td> 0.2437</td>

123

<td>Ingredients</td><td colspan="5">Quantities (kg) per cWa ^^^ o INDUSTRIAL</td>

<td rowspan="2"></td><td rowspan="2">Example 110</td><td rowspan="2">Example 111</td><td>Example</td><td>Example</td><td>Example</td>

<td> 112</td><td> 113</td><td> 114</td>

<td>Ascorbic acid</td><td> 0.2400</td><td> 0.2400</td><td> 0.2400</td><td> 0.2400</td><td> 0.2400</td>

<td>Food Grade Antioxidant Tocopherol-2</td><td> 0.1161</td><td> 0.1161</td><td> 0.1161</td><td> 0.1161</td><td> 0.1161</td>

<td>Ferrous sulfate</td><td> 0.0929</td><td> 0.0929</td><td> 0.0929</td><td> 0.0929</td><td> 0.0929</td>

<td>Vitamin premix</td><td> 0.065</td><td> 0.065</td><td> 0.065</td><td> 0.065</td><td> 0.065</td>

<td>Zinc sulfate monohydrate</td><td> 0.057</td><td> 0.057</td><td> 0.057</td><td> 0.057</td><td> 0.057</td>

<td>Manganese sulphate</td><td> 0.0454</td><td> 0.0454</td><td> 0.0454</td><td> 0.0454</td><td> 0.0454</td>

<td>Copper sulfate with mineral mixture</td><td> 0.0353</td><td> 0.0353</td><td> 0.0353</td><td> 0.0353</td><td> 0.0353</td>

<td>30% beta carotene</td><td> 0.0056</td><td> 0.0056</td><td> 0.0056</td><td> 0.0056</td><td> 0.0056</td>

<td>Chromium Chloride</td><td> 0.0013</td><td> 0.0013</td><td> 0.0013</td><td> 0.0013</td><td> 0.0013</td>

<td>Sodium molybdate</td><td> 0.0012</td><td> 0.0012</td><td> 0.0012</td><td> 0.0012</td><td> 0.0012</td>

<td>Potassium iodide</td><td> 0.001</td><td> 0.001</td><td> 0.001</td><td> 0.001</td><td> 0.001</td>

<td>Sodium selenite</td><td> 0.0004008</td><td> 0.0004008</td><td> 0.0004008</td><td> 0.0004008</td><td> 0.0004008</td>

<td>Citric acid</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

124

<img file="MX347477B_D0024.tif" />

<td>Ingredients</td><td colspan="5">Quantities (kg) per cad ^^^^^</td>

<td rowspan="2"></td><td rowspan="2">Example 110</td><td rowspan="2">Example 111</td><td>Example</td><td>Example</td><td rowspan="2">Example 114</td>

<td> 112</td><td> 113</td>

<td>Potassium hydroxide</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Dry magnesium sulfate</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Ultramicronized Tricalcium Phosphate</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Ascorbic acid</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td><td>As necessary</td>

<td>Water</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td><td>CS</td>

125