Apparatus for dividedly taking particle

[Detailed Description of the Invention]

(b) Field of the Invention This invention relates to a particle aliquot apparatus. Various particles, such as a cell, a blood cell, and a chromosome, are related with the particle aliquot apparatus which is optical and can carry out judgment extraction by the electric method in detail. (Ro) PRIOR ART The drop which divides from particle To or Flow and falls continuously below from the former, Based on the optical characteristic of each particle in the particle To and Flow, predetermined potential is respectively electrified with the electrode for electrification, and the particle aliquot apparatus which carries out deviation fall with a pair of deflecting electrode boards allocated in the fall way, and isolates each particle preparatively is used for preparative isolation of the red +In ball in various cells or blood, blood platelets, white corpuscles, etc. This conventional particle aliquot apparatus was shown in Drawing 6. In a figure, it is a sheath flow cell (To and Flow which descend from 21 are divided by the drop so that one particle may be included in Extensive with a vibrator (5).). The optical detection system (3) is set to the fluid jet (21), and it is the optical characteristic here. When required particles are detected based on (being distinction at dispersion light intensity about a particle size in judgment with red corpuscles and blood platelets), Place City pulse (two positive or negative kinds) is applied to the electrode for electrification (4) at the moment of being divided by the drop, Thereby, S Muddy drop is charged in this particle, a Pull rose Falling orbit is bent in the direction of either with a pair of electrode boards for Editing (7△) (7B) by which countering arrangement was carried out via the fall way, and it is extracted in recovery container [91[91. And judgment extraction of two kinds of particles is attained by giving a positive or negative electric charge suitably to Particle corresponding to the difference in the optical characteristic. (8) is a Drainage container which collects the drops of the non-electric charge which does not mean judgment extraction. (C) In the problem which an invention tends to solve, however the conventional device, positive and two negative kinds of the kind of particles in which judgment extraction is possible are practical use limits fundamentally by the polarity of an electrification pulse. Of course, although the proposal which the voltage of an electrification pulse is changed, and is deflected and classified to four kinds (Engineering quantity * voltage, the right low voltage, negative high voltage, and negative low voltage) is also made, the purity of recovery particles puts in practical use by being low, and is not that of nonglutinous rice. This invention is made in view of this situation, and it tends to provide the particle aliquot apparatus which can carry out judgment extraction of two or more kinds of particles efficiently, without controlling the height of electrification voltage. (D) The drop which according to the invention of the means hiding lever for solving a problem divides from particle To or Flow and falls continuously below, Corresponding to the optical characteristic of each particle in the particle To and Flow, predetermined potential is respectively electrified with the electrode for electrification, It is constituted and becomes so that deviation fall may be carried out with a pair of deflecting electrode boards allocated in the fall way and each particle may be isolated preparatively, and it is between the above-mentioned deflecting electrode board and the electrode for electrification, The particle aliquot apparatus which attaches the micro nozzle which makes a carrier fluid drop collide with the falling electrification drop, and Transfer ?11 b Deals in the drop position of the electrification drop almost in parallel to a deflecting electrode board is provided. This invention is characterized [ biggest ] by the point constituted so that the fall drop electrified in positive or negative either could be physically moved in parallel with the deflecting electrode board before the deviation with a deflecting electrode board using the micro nozzle which carries out discharge of the drop which can serve as carrier fluid of particles. This micro nozzle may be constituted so that it may consist of one nozzle and a drop position can be moved to one way from a reference position, and it may be constituted so that it may consist of two nozzles by which countering arrangement was carried out and a drop position can be bidirectionally moved from a reference position by these changes. it is suitable to use a physiological saline etc. as J3 and carrier fluid, and a size of a discharged liquid drop is suitable until it is comparable as a fall drop. It will become difficult to deviate if not much large. . It is usually necessary to control suitably 0N10FF of collision operation of the drop by these micro nozzles corresponding to the optical characteristic of the particles contained in particle To and Flow before division. for example, the deviation judgment by right and the negative charge which resembles control of a standard drop position and a move drop position (both directions), respectively, and corresponds based on the optical characteristic of each particle in using the particles of 6 balances as an opposite house -- an association -- Climbing -- by things, it is accurate and judgment extraction is attained. (Ho) Action In the particle aliquot apparatus of this invention, it is divided, and by the collision of the carrier fluid drop by a micro nozzle, the electrified drop moves a drop position almost in parallel, and falls to a deflecting electrode board, and judgment extraction is further carried out in response to a deviation according to right and the negative one of that electric charge. Therefore, a separation setup of many fall orbits can be carried out at a seed by combining and controlling the change of 0N10FF of positive [ of an electric charge ], a negative change, and collision operation of a carrier fluid drop, and its collision direction. And by setting up a recovery container in the shape of two dimensions corresponding to this fall orbit, judgment extraction of two or more sorts of particles exceeding two sorts will be made. It puts and is EXAMPLE. (1) shown in Drawing 1 is a composition explanatory view showing one example of the particle aliquot apparatus of this invention. The sheath flow cell (2) to which a particle aliquot apparatus (1) drops particle To and Flow continuously in a lower part from a fluid jet (21) in a figure, The optical detection system (3) which is provided with light source 01 and euphotic machine ■, and monitors the optical characteristic of the particles in particle To or Flow, The vibrator (5) which gives vibration to particle To and Flow which are attached to a sheath flow cell (2) and descend, and it divides to a drop, The electrode for electrification (4) which may be charged positive or negatively in the divided fall drop, and a pair of deflecting electrode boards which can deflect the fall orbit of electrification fall drop □□□ (7A) (it comprises 7B>.) And a pair of micro nozzles which breathe out a carrier fluid drop periodically between an electrode for electrification (4), and a deviation Advertisement board (7△) (7B), and may make it collide with it in the amount of drops (as 6A> (6B) shows in Drawing 2, allocated face to face.), As for a discharge direction of a drop from a micro nozzle, it is, as shown in Drawing 3, and a gap is also a deflecting electrode board (set up to become parallel to 7A> (7B).). the inside of a figure and (8) -- Drainage container (9A) < -- 9B> shows the recovery container group of each particle, respectively, and (41) is an input terminal of an electrification pulse. In this device (1), fall of a drop is first performed by division of particle To or Flow as usual. Under the present circumstances, a positive or negative electric charge is given to the drop containing the particles meant corresponding to the output of an optical detection system by the electrode (4), and an electric charge is not given to other drops. Thus, the falling drop (3) is a micro nozzle (although extracted by the existence of the electric charge, and the polarity of an electric charge by fall To orbit in a figure at the container located in a Drainage container (8), a recovery container group (9 delta), or the center of (9B) in the state of OFF of 6A> (6B)). A fall orbit will be extracted by turning on a micro nozzle (6A) to an electrification drop by either of the containers which carries out parallel translation to the position in [ C ] a figure, and is located behind a recovery container group, A fall orbit will be extracted by turning on a micro nozzle (6B) by either of the containers which carries out parallel translation to the position in [ a ] a figure, and is located most ahead. the discharge of the drop from one of micro nozzles and a collision -- drop position Furthermore of an electrification fall drop mouth -- a fall orbit -- a deflecting electrode board -- < -- 7A (7B) The state of moving in parallel was shown in Drawing 2. A figure destructive line shows the case where it is made to reverse-move. The relation between each container group (9A) (9B), and the move direction by drop collision and a deflection direction with a deflecting electrode board was shown in Drawing 4. Thus, in a particle aliquot apparatus (1), judgment extraction of six kinds of particles can be carried out. Judgment of right and a negative charge and judgment of ON/OFF of a micro nozzle, According to the output in an optical detection system, it can carry out in the control part by a microprocessor, may monitor for every timing of drop division of dispersion light intensity, and may combine and control to separate these fall orbits based on the intensity of this. Drawing 5 is 4th [ The ] figure equivalent figure in the same particle aliquot apparatus as the above constituted so that a micro nozzle might be constituted from one and a drop position could be moved to one way, and can carry out judgment extraction of four kinds of particles in this case. (A) EFFECT OF THE INVENTION According to this invention, judgment extraction of many particles of a seed can be carried out from the sample containing various particles. And judgment extraction can be performed efficiently, without controlling the height of electrification voltage, and it is advantageous practically.

[Brief Description of the Drawings]

Drawing 1 is Show Thu ° about one example of the particle aliquot apparatus of this invention (a composition explanatory view and Drawing 2). The explanatory view and Drawing 3 showing the move state of the electrification fall drop by a micro nozzle similarly, 4th [ The ] figure equivalent figure [ in / in the explanatory view and Drawing 5 in which the explanatory view and Drawing 4 showing physical relationship with a micro nozzle, a deflecting electrode board, and the electrode for electrification similarly are the same, and showing movement of a container group and a fall orbit and a relation with a deflection direction / other examples ], and Drawing 6 are composition explanatory views which illustrate the conventional particle aliquot apparatus. (1) ...... Particle aliquot apparatus (2), ...... Sheath flow cell (3), ...... Optical detection system (4) ...... The electrode for electrification, (5) ...... Vibrator (6A) (6B) (9B> ...... [ A recovery container group, A ] ...... electrification fall drop) ...... Micro nozzle (7A) (7B) ...... A deflecting electrode board, (8) ...... Drainage container (9A) Drawing 7 Drawing 2 eta To Drawing 4 7B D