Germfree type continuous centrifugal separation system

[Detailed Description of the Invention]

The present invention relates to a sterile type continuation centrifugal separation system. These days, it is necessary with progress in the research on a tissue culture to separate a lot of samples continuously. Without showing the sectional view of the centrifuge of the conventional sterile type continuation rotor system, and making air touched with a sample, Drawing 1 is sterile and carries out centrifugal separation processing. In Drawing 1, the supernatant liquid tank by which 1 stores a sample tuck, 2 stores supernatant liquid, and 2a stores supernatant liquid 2, the rotor body which rotates 3 by drive 11.1 (not shown), and 4 are the covers of rotor body 3. Eye five: It is supported by supporter 14 for piping supported by the nonrotation state, and sample tuck 1 is open for free passage, carry out the opening of the lower end to the rotation center part inside rotor body 3, and it carries out continuous supply of the sample to rotor body 3. 7 is Water seal solution, and while rotor body 1 rotates the perimeter portion of piece 15 of Water seal of a Cross section form, the circumference section of the cartridge fixed to supporter 14 by carrying out hermetic sealing of the upper end in liquid seal room 12 is in it as Every so that it may Seal water like illustration. 8 is a shield plate and the sediment in which 9 was centrifuged. 13 is a guide of centrifuged supernatant liquid 2, and it is attached to the perimeter of piping 5, and has the shape like the runner of centrifugal A pot, and rotor body 3 rotates, Supernatant liquid 2 which has Energy of the speed after sediment 9 was separated is guided from the slot around guide 13, and is guided into supernatant liquid tank 2a. That is, it is as the Bong operation Keep away which attracts supernatant liquid 2 relatively. And a sample to be dissociated passes through piping 5 from sample tank 1, and it is at the center in rotor body 3, namely, -- being supplied by the pumping action which attracts the sample by gravity and rotor body 3 rotating -- an arrow -- like -- Handful of Sega 6 -- a connoisseur -- it is centrifuged nine times, and sediment 9 is caught in rotor body 3, and it is shown to supernatant liquid 2 to it at supernatant liquid tank 2a. And in order to be unable to touch a sample to air at a sterile type, a liquid seal all line is with Water seal solution 7 at the liquid / - Le room 12 of the rotor body 3 upper part. A Yo Si decision of the time (sedimentation time) T generally taken to separate a sample on the other hand is made at (1) type. T= -.....- ....................................... (1) However, S : the size of particles Into: shape and number of rotations -- [ -- in the decided constant, however this sterile type continuation rotor body, since it has a runner of the above-mentioned self-priming type, the supply flow velocity of a sample has the problem that it will be decided at the flow velocity of rotor body 3, and cannot decide on time T to be shown in (1) type arbitrarily. For this reason, in order to secure necessary centrifugal time, when there is much quantity which controls the amount of supply of a sample and is attracted by a runner rather than the amount of supply, The inside of rotor body 3 serves as negative pressure, and has the fault that centrifugal separation time of an impossibility [ saying / inhaling air and not making external air touched from the liquid seal part of liquid seal room 12 ], i.e., predetermined, is not securable. the present invention is made in view of the above-mentioned situation, and it is It was for the purpose of providing the sterile type continuation centrifugal separation system which can control sample supply 1 and can secure predetermined centrifugal time without making the inside of a rotor body into negative pressure. The sample feeding means which carries out continuous supply to the rotor body central part where the sterile type continuation centrifugal separation system of the present invention rotates a sample to be dissociated via piping, The supernatant liquid ejecting means which closes the supernatant liquid centrifuged from the above-mentioned sample supplied by the sample feeding means in the above-mentioned rotor body via a water seal device so that the atmosphere may not be contacted, and is discharged at a sterile ceremony is established, The sterile air entrance which was independent, or used together and opened sterilization liquid or a high efficiency filter for free passage to the atmosphere to pass is opened for free passage in the above-mentioned rotor body. Below, a same sign shows the former and a said division article using an example, the sterile type continuation centrifugal separation system of the present invention is omitted, and explanation of the structure of the portion explains it with Drawing 2. When there is little quantity of a separation sample, as shown in Drawing 1, sample tank 1 is placed on the table of the rotor body 3 upper part etc., but this example is a case where the quantity of a sample places many sample tanks 1 on a floor. 16 is open for free passage to the drive motor of rotor body 3, 17 is opened for free passage by the tube rigorous training type pump, and 18 is opened for free passage with the control panel and x9h sample feed pipe by piping (not shown) in sample tank 1 and rotor body 3. to sample feed pipe 19, a sample is sucked up from sample tank 1, and in order to secure predetermined centrifugal time, the amount of supply of a sample is controlled -- Chi-The(ing) -- the time -- formula pump 17 -- taking -- I have been asked. And if the sample amount of supply is restricted in order to secure predetermined centrifugal time, in order to prevent external air from the inside of rotor body 3 serving as negative pressure, and being inhaled at liquid seal room 12, Air as shown in Drawing 3 showing the details of the A section, after sterilization tank 10 purifies contaminated air is supplied, and pressure difference is canceled. Thus, the sterile type continuation centrifugal separation system of this example is constituted so that the air which passed the inside of sterilization liquid according to the negative pressure in a rotor body may be supplied and pressure difference may be canceled, it is that of 9, and controls the sample amount of supply, and can secure predetermined centrifugal separation time. Drawing 4 shows the A section of Drawing 2, i.e., other examples of a sterile air entrance, and is a substitute of sterilization tank 10 of Drawing 3, a 0.3-micro particle -- 99.9% The air which attached and purified high efficiency filter 11 of the Hepa (brand name Hg:pA) filter which can be caught is supplied, and the negative pressure in a loader body is canceled, and it is It is and has the same operation effect as the above-mentioned example. If it uses together with A helmet and sterilization filter 10, a still bigger effect is expectable. As described above, it is a sterile type continuation centrifugal separation system (it has the effect that the sample amount of supply can be controlled and predetermined centrifugal time can be secured without making the inside of dl rotor body into negative pressure.) of the present invention.

[Brief Description of the Drawings]

The explanatory view of the example of the sterile type continuation centrifugal separation system of the present invention, Drawing 3, and Drawing 4 of the important section sectional view of the sterile type continuation centrifugal separation system of the former [ Drawing / 1 ] and Drawing 2 are detail views of the different To do structure of the A section of Drawing 2, respectively. 2 ... supernatant liquid, 3 ... rotor body, 5 ... piping, 7 ... Water seal solution, 10 ... sterilization tank, 11 ... high efficiency filter, 12 .. liquid seal room. An applicant's for a patent name Hitachi Koki Co., Ltd. Oh 1 figure 263- ; fz figure T3rb tarb ↓