Disposable reagent pack and analyzer using the reagent pack

The present invention relates to a disposable reagent pack and an analyzer using the reagent pack.

As a disposable processing device, a device (container) including an array of integrally configured chambers, an integrally configured cover insert, and a disposable pipette tip has been proposed (see Patent Document 1). This device consists of one or two containers for processing samples, one chip, and a waste liquid container section.<patcit num="1"><text>Japanese Unexamined Patent Publication No. 11-9258</text></patcit>

<p> However, the above-mentioned device is only a so-called disposable device (container), and does not contain a solution such as a reagent. In addition, since the disposable device according to Patent Document 1 above proposes only the configuration of the disposable device, problems related to automation of the analyzer, problems related to preparation, and problems related to disposal method after the test remain unsolved. ..</p><p> An object of the present invention is to provide a disposable reagent pack that is easy to manage and handle and can be handled without mistakes, and an analyzer using the reagent pack.</p>

<p> The disposable reagent pack according to one aspect of the present invention includes a container for accommodating a required number of disposable chips, at least one waste liquid container, and at least one solution container filled with a required type of solution, and is said to be disposable. The container for accommodating the chips, the waste liquid container portion, and the reagent container are integrally formed.</p><p> The analyzer according to another aspect of the present invention includes a reagent pack mounting unit on which the above reagent pack can be placed, and an analysis unit that analyzes the results of analyzing a sample using the reagent pack. It is a feature.</p>

<p> According to the present invention, it is possible to provide a disposable reagent pack that is easy to manage and handle and can be handled without mistakes, and an analyzer using the reagent pack.</p>

For example, when genetic testing is performed using an analyzer, a sample (that is, a sample), several types of reagents and buffers (solutions), several disposable chips, a reaction vessel, etc. are set in a clean state in the analyzer. There is a need. In addition, after the inspection is completed, it is necessary to remove dirty chips, waste liquids, dirty reaction vessels, etc. from the analyzer and dispose of them, remove reagents and buffer bottles, and return them to the cold storage. In such work, dust may be mixed in the reagent or buffer bottle, the reagent may be altered due to carryover, etc., the expiration date management of the reagent may be ambiguous, or the type or concentration of the reagent or buffer may be incorrect. There is also the possibility that it will end up. In the present invention, a disposable reagent pack (hereinafter referred to as "reagent pack") for solving the above-mentioned problems and making it easy for the user to use and making mistakes difficult, and an analyzer using the reagent pack will be described in detail below. To do. As an analyzer, a configuration in which a nozzle transfer mechanism for handling a sample and a solution has a linear motion structure for linearly moving along the XYZ axes will be mainly described, but as another embodiment, the arm is rotated. A configuration having a rotary vertical movement structure for moving reagents and the like is also briefly described.

Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a diagram relating to an analysis system including an analyzer according to the present invention. As shown in FIG. 1, the analysis system according to the present invention includes an analysis device 26, an electrical component 113, and a control unit (for example, a main body of a personal computer) 31. The details of the analyzer 26 are as shown in FIG. The electrical component 113 includes a Z-axis driver controller 27, a Y-axis driver controller 28, a chip tip position sensor amplifier 29, an X-axis driver controller 30, a sample table transfer driver controller 46, and a microscope driver controller 47. There is. For example, a keyboard 34 and a pointing device 32 are connected to the control unit 31 as an input device, and a display 33 or a printer (not shown) is connected as an output device. FIG. 2 is an overall view of the analyzer according to the present invention. The description of each part described in FIG. 2 shall be given at the time of explanation of the operation, and is omitted here.

First, before explaining the operation of the analyzer according to the embodiment of the present invention. The configuration of the disposable reagent pack according to the embodiment of the present invention will be described with reference to FIG. As shown in FIG. 3, the reagent pack 5 according to the present embodiment includes a sample container 8, chip containers 48 to 55 in which the chip 6 is set, and a solution storage unit filled with a predetermined solution 7 for analysis. 56 to 63, the waste liquid container portion 13, and the sample pretreatment container portion 64 are integrally formed. Also. A solution storage portion lid film 42 for preventing leakage of the solution or the like is provided above the solution storage portions 56 to 63. Further, it is preferable to provide the reagent pack cover 43 so as to cover the entire upper part of the reagent pack 5. In FIG. 3, (a) is a top view, (b) is an AA sectional view of (a), and (c) is a side view. Further, in the configuration of FIG. 3, it is not necessary that all the containers are integrally formed, and at least the chip container portions 48 to 55, the solution storage portions 56 to 63, and the waste liquid container portion 13 may be provided. .. As will be described later, since the analysis after setting the reagent pack 5 in the analyzer 26 is automatically performed, a positioning hole 45 is provided in order to accurately position the reagent pack 5 in the analyzer 26. Further, it is preferable that the reagent pack 5 is attached with a barcode label 44, which is identification information for specifying the type of the reagent pack 5, the type of the sample to be packed, and the like. It is preferable that the barcode label 44 contains readable characters and can be read by the operator without a barcode reader. Further, it is preferable that the information on the barcode label 44 includes information such as the lot number of the reagent pack, the expiration date, the analysis item, and the analysis procedure identification code. Instead of the barcode label 44, any means such as an IC tag that can identify predetermined information may be used. Since the reagent pack 5 according to the embodiment of the present invention includes the solution as the reagent, the necessary container is integrally formed. You can easily analyze and clean up after the analysis.

Hereinafter, the operation of the analyzer according to the present invention will be described mainly with reference to FIG. Except for the control including the computer 31, all the devices according to the embodiment of the present invention are arranged on the device base plate.

(Preparation) 1) Turn on the computer 31 (including monitor 33), analyzer 26, and electrical equipment 113, and start the system (computer OS startup, control program startup, analyzer initialization, etc.). Do. 2) The operator removes the reagent pack 5 and the DNA reaction vessel 15 corresponding to the predetermined analysis items from the cool box. The DNA reaction vessel 15 is divided into two bodies, and the sample tank 16 is sandwiched and fixed. 3) One or more reagent packs 5 are set in the reagent pack mounting section 108 of the analyzer 26, and the DNA reaction vessel 15 is set in the sample table 14. At this time, remove the lids of the reagent pack cover 43 and the DNA reaction vessel 15 (not shown) in the device cover or in an environment where dust and dirt do not enter the reagent pack 5, and remove the sample corresponding to the reagent pack type. Dispense a predetermined amount into the sample container 8 of the reagent pack. The reagent pack 5 is fixed and positioned by fitting the positioning holes of the reagent pack 5 into the positioning pins 105 and 106 of the reagent pack mounting portions 108 provided on the X-axis linear motion robot 4 of the apparatus. .. The DNA reaction vessel 15 is fixed and positioned on the sample table 14.

(Start of operation of the device) 4) The operator instructs the control unit 31 of the analyzer 26 to start the operation. As a result, the following operations are automatically performed by the computer (control unit) 31. 5) The reagent pack mounting unit 108 moves, and the information on the barcode label 44 attached to the reagent pack 5 is read with a barcode reader (not shown). The expiration date of the reagent pack 5 is confirmed from this read information, and an operation procedure based on the read reagent information is selected from a plurality of operation procedures programmed in the analyzer in advance. If the computer 31 can communicate with the outside by a communication means (for example, the Internet), the information of the barcode label 44 of the reagent pack 5 can be sent to the external (or higher) computer for optimal and up-to-date analysis. It is also possible to download the operating procedure of the device and operate the analyzer 26 according to the operating procedure. Furthermore, it is also possible to collate and analyze the analysis results using the information of the external (upper) computer by using the communication means.

(Chip mounting) 6) By operating the Y-axis linear motion robot 107, the nozzle 20 mounted on the Z-axis linear motion robot 21 is moved onto the unused chip 6 on the reagent pack 5. Similarly, the X-axis linear motion robot 4 also operates to move the nozzle 20 onto the unused tip 6 on the predetermined reagent pack 5. Eight chips are mounted on the reagent pack 5 shown in FIG. 3, but in FIG. 3, the maximum number of chips that can be mounted on the reagent pack 5 is eight, and according to the number of chips used for analysis. It is possible to reduce the number of chips. In that case, instead of preparing all the chips in the chip container portions 48 to 55 of the reagent pack 5, there may be a chip container portion in which the chips are not contained. When nine or more chips 6 are required, for example, the reagent pack 5 may be configured in two rows. As the number of chips 6 increases, a 3-row configuration or a 4-row configuration may be used. Similarly, the solution storage units 56 to 63 are also provided at eight locations like the chip container units 48 to 55, but the solution storage units 56 to 63 do not contain the solution depending on the type of solution required for analysis. A solution storage unit may be provided. If the amount of one type of solution used exceeds the capacity of one solution storage unit, it can be dealt with by putting the same type of solution in a plurality of solution storage units. Further, when 9 or more kinds of solutions are used, it is possible to cope with the case where the reagent pack 5 is configured in 3 rows or 4 rows as in the case where the number of chips is increased. 7) The rotation of the timing pulleys 23 and 24 causes the timing belt 22 to operate, and the Z-axis linear motion robot 21 operates to lower the nozzle 20 by a predetermined amount and push it into the tip 19 (6) to push the nozzle. 20 and tip 19 fit together. 8) When the Z-axis linear motion robot 21 operates, the tip 19 fitted with the nozzle 20 rises to the upper point position (origin sensor position: that is, the position before the nozzle 20 descends) ((a) in FIG. 4). ) Is rising).

(Sample suction) 9) The Y-axis linear motion robot 107 operates to move the chip 19 onto a predetermined sample container portion 8 in the reagent pack 5. 10) When the Z-axis linear motion robot 21 operates, the tip 19 is lowered, and the tip of the tip 19 is positioned at the very bottom of the sample container portion 8. 11) For example, by driving a syringe pump 1 connected to a syringe 2 connected via a Teflon tube ventilation tube 25, sample 9 (hereinafter, also referred to as reagent) is sucked into the tip 19. To do. 12) After the suction of the sample 9 is completed, the Z-axis linear motion robot 21 is operated to raise the tip 19 to the upper point position.

(Removal of chip from sample pretreatment) 13) If pretreatment of sample 9 is required, perform the following procedure. Here, the "pretreatment of the sample" means a treatment such as dilution of the sample concentration and addition of a sample modifier (for example, a surfactant). 14) Discharge the aspirated sample to the sample pretreatment container 64 in the reagent pack 5, and discard the chip contaminated with the sample in the original place in the reagent pack 5 (at this time, it is attached to the Z-axis linear motion robot. Drive the chip removal unit 12 and push the upper edge of the chip 19 to remove the chip from the nozzle 20), and attach a new chip 6 (19) to the nozzle 20 ((f) in FIG. 4). 15) Using the new tip 19 attached to the nozzle 20, the predetermined solution contained in the solution storage portions 56 to 63 of the reagent pack 5 is sucked ((g) in FIG. 4). At this time, a hole is made in the solution storage portion lid film 42 of the solution storage portions 56 to 63 at the tip of the tip, the tip is lowered to a predetermined height, and the solution is sucked (piercing suction). The solution storage lid film 42 is mainly composed of aluminum foil, and is easily perforated at the tip of the chip 19 such as a film coated with a resin such as polypropylene, polyethylene, polyester, or polyolefin to prevent the solution from being altered. It is preferable to use a material that can be used. In addition, since the material of the reagent pack 5 itself is the same or a resin that can be welded, the solution storage lid film 42 and the main body of the reagent pack 5 are prevented from leaking water or being mixed with dust or dirt due to ultrasonic welding or the like. It is watertightly sealed. 16) After the suction of the sample 9 is completed, the tip 19 is raised and moved to the upper part of the sample pretreatment container portion 64. 17) When the Z-axis linear motion robot 21 operates, the tip 19 is lowered, and the tip of the tip 19 is positioned at the very bottom of the sample pretreatment container portion 64. 18) Discharge the solution in chip 19 and discharge at least 1/2 of the total volume of the sample volume discharged in advance and the solution discharged this time (of course, the upper limit is the total capacity or the maximum capacity of the chip, whichever is smaller. Yes) is sucked and then discharged. This suction and discharge is repeated multiple times (preferably 10 times or more, but the number of times and the flow velocity need to be changed depending on the sample, the type of solution, the amount, the degree of stirring, etc.), and the sample and the solution are stirred ((b) in FIG. 4). ). 19) After the stirring of the sample and the solution is completed, a predetermined amount of the sample is sucked using the same tip 19. 20) After the suction of the sample is completed, the Z-axis linear motion robot 21 is operated to raise the tip to the upper point position.

(Solution discharge) 21) By operating the Y-axis linear motion robot 107, the chip 19 in the state where the sample is sucked is moved onto the hole for the sample tank 16 provided in the DNA reaction vessel 15. 22) By operating the Z-axis linear motion robot 21, the chip is lowered to the edge height of the hole for the sample tank 16 provided in the DNA reaction vessel 15 ((c) in FIG. 4). 23) Drive the syringe pump 1 and discharge the entire amount of the sample in the chip 19 into the sample tank 16 of the DNA reaction vessel 15. 24) After the discharge is completed, the Z-axis linear motion robot 21 is operated to raise the tip 19 to the upper point position.

(Chip removal) 25) Discard the chip 19 contaminated with the sample in its original location in the reagent pack 5.

(Solution reaction) 26) The sample dispensed into the DNA reaction vessel 15 is fixed to the sample tank 16 by a solution driving means (not shown) while keeping the temperature at about 50 ° C by a heater (not shown). Initiate a hybridization reaction with the DNA probe shown in FIG. FIG. 5 is a diagram showing an example of a reaction vessel. In FIG. 5, the reaction vessel 109 is provided with a DNA reaction vessel sample tank 111 (referred to as sample tank), and a DNA probe 112 is provided inside the sample tank 111. The circular spot on the DNA microarray 112 is the DNA probe. Reference numeral 110 is a lid for the sample tank 111. The hybridization reaction requires a reaction time of several minutes to several hours.

(Tip mounting and tip position measurement) 27) Attach the new tip 6 in the reagent pack 5 to the nozzle 20 ((f) in Fig. 4). Then, when the Y-axis linear motion robot 107 operates, the nozzle 20 on which the new tip is mounted is moved to the tip tip position sensor 11 position attached to the tip tip position sensor holding plate 10, and the tip position of the tip 19 is moved. To measure. Since the measurement of the tip position of the chip 19 is irrelevant to the subject of the present invention, detailed measurement methods and the like will be omitted, and the following description will be made assuming that the tip position of the chip 19 can be accurately measured. ..

28) When the Z-axis linear motion robot 21 operates, the chip 19 rises to the upper point position.

(Suction of waste liquid) 29) The hybridization reaction started from step 26) is completed. The reaction time is a time necessary and sufficient for the hybridization reaction. 30) When the Y-axis linear motion robot 107 operates, the chip 19 after the tip position is measured by the chip tip position sensor 11 moves to the vicinity of the edge of the hole for the sample tank provided in the DNA reaction vessel 15 (FIG. 4). (C)). 31) When the Z-axis linear motion robot 21 operates, the chip 19 after measuring the tip position descends to a height of about 0.1 mm from the surface of the sample tank 16 provided in the DNA reaction vessel 15 (Fig.). 4 (d)). 32) Operate the syringe pump 1 to suck the solution 17 on the sample tank 16. 33) When the Z-axis linear motion robot 21 operates, the nozzle 20 that sucks the solution rises to the upper point position.

(Waste liquid, tip removal, installation) 34) By operating the Y-axis linear motion robot 107, the tip 19 moves to the upper part of the waste liquid container portion 13 arranged on the reagent pack 5. 35) When the Z-axis linear motion robot 21 operates, the tip of the chip 19 descends to at least 5 mm or more below the upper surface of the waste liquid container. 36) Operate the syringe pump 1 to discharge the solution in the tip 19 into the waste liquid container portion 13. Here, the discharge amount of the solution is the total amount sucked ((e) in FIG. 4). 37) Discard the chips contaminated with the waste liquid in their original location in the reagent pack 5. At this time, the tip removal unit 12 attached to the Z-axis linear motion robot is driven, and the upper edge (fitting portion with the nozzle 20) of the tip 19 is pushed downward to remove the tip from the nozzle 20. Then, a new tip 6 (19) is attached to the nozzle 20 ((f) in FIG. 4).

(Washing) 38) The solution (washing buffer) set in the reagent pack 5 is (solution suction), (solution discharge), solution-driven washing by a solution-driven means (not shown), (chip removal), (chip). Cleaning after the hybridization reaction is performed by performing each of the steps of mounting and tip position measurement), (waste liquid suction), and (waste liquid, tip removal, and mounting). The cleaning operation can be performed several times while exchanging several types of solutions (cleaning buffer). It can also be repeated several times using the same solution (wash buffer).

(Measurement) 39) The DNA reaction vessel 15 is moved by the linear motion robot 41 for transfer of the sample table under the objective lens 40 of the microscope by the linear motion robot 41 for transfer of the sample table, and the hybridization result is optically measured. For example, in the case of fluorescence observation, the sample DNA hybridized with the DNA probe inside the sample tank 16 is irradiated with the excitation light emitted from the light source 38 through the microscope translucent tube 18, and the generated fluorescence is emitted. The hybridization result is measured by imaging with the CCD camera 39. This is the end of the automatic operation by the 26 control units 31 of the analyzer.

(Post-treatment) 40) For the operator, remove the reagent pack 5 and the DNA reaction vessel 15 set in the analyzer 26.

41) Dispose of the removed reagent pack 5 and DNA reaction vessel 15 by treating them according to the treatment method specified by the local government. 42) After working on the analyzer, initialize it, etc., and turn off the power of the computer 31 (including the monitor 33), the analyzer 26, and the electrical component 113. 43) The obtained image data can be analyzed using another computer. It is also possible to send image data together with the information of the barcode label attached to the reagent pack 5 to a higher-level computer using the Internet or the like, and perform analysis processing using a data analysis service, a genetic information collation service, or the like.

In the above embodiment, the XYZ axis linear motion type analyzer and the reagent pack applicable to the analyzer have been described, but it can also be applied to the following rotary motion type analyzer. The linear motion analyzer has a high degree of freedom in the stop position and can be constructed by using a general-purpose robot, but it has many components and is relatively large. It is also known that it is difficult to increase the operating speed. On the other hand, in the rotary motion type analyzer (Fig. 6), the degree of freedom of the stop position is considerably limited, and although a general-purpose robot is not found, the number of component parts can be reduced and the size can be reduced. It is also known that the operating speed can be easily increased.

FIG. 6 is a diagram showing a schematic configuration of a rotary motion type analyzer according to another embodiment of the present invention. Further, FIG. 7 is a diagram showing a schematic configuration of a disposable reagent pack 104 applicable to the device.

In the rotary motion type analyzer, the sample (reagent) is moved between the reagent pack wheel 71 and the DNA reaction vessel wheel 66 including the incubator by the rotary motion of the nozzle transfer arm 68 about the rotation axis 87 of the nozzle transfer arm. It is carried out. Therefore, the arrangement of each container portion of the reagent pack 104 according to the present embodiment is arranged at a position that matches the turning radius of the nozzle transfer arm 68. First. The schematic configuration of the reagent pack 104 will be described with reference to FIG. 7.

As shown in FIG. 7, the reagent pack 104 according to the present embodiment is a solution container filled with a sample container 79, chip containers 78, 81 to 86, 101 in which chips are set, and a predetermined solution for analysis. Parts 80, 88 to 91, 93, 94, 99, waste liquid container part 95, and sample pretreatment container part 96 are integrally formed. Also. A solution storage portion lid film 92 for preventing leakage of the solution or the like is provided above the solution storage portions 80, 88 to 91, 93, 94, 99. Further, it is preferable to provide the reagent pack cover 100 so as to cover the entire upper part of the reagent pack 104. In FIG. 7, (a) is a top view, (b) is a front view, and (c) is a side view. Further, in the configuration of FIG. 7, it is not necessary that all the containers are integrally formed, and at least the chip containers 78, 81 to 86, 101 and the solution storage portions 80, 88 to 91, 93, 94, 99, and so on. It is the same as the above-described embodiment that the waste liquid container portion 95 may be provided. Further, as in the above embodiment, since the analysis after setting the reagent pack 104 in the analyzer is performed automatically, the positioning hole and / and the notch 98 are provided in order to accurately position the reagent pack 104 in the analyzer. It is provided. Further, it is preferable that the reagent pack 5 is attached with a barcode label 97 which is identification information for specifying the type of the reagent pack 104, the type of the sample to be packed, and the like.

The schematic configuration of the rotary operation type analyzer to which the reagent pack as described above is applied will be described with reference to FIG. Since the operation of the rotary operation type analyzer (hereinafter referred to as analyzer) is the same as that of the above embodiment, the description thereof will be omitted.

The reagent pack 73 (104) is installed on the reagent pack support 77 by fitting the positioning pins 74 and 102 of the analyzer and the positioning hole 98 of the reagent pack 104. The reagent pack wheel 71 of the analyzer can rotate around the reagent pack wheel rotation shaft 75 as a rotation axis. A nozzle 72 provided at the tip of the nozzle transfer arm 68 is lowered by a vertical drive unit (not shown) to be fitted and attached to the chip 78. When the nozzle 72 to which the tip 78 is attached rises and reaches the upper point position, the nozzle transfer arm 68 rotates and the tip position is detected by the tip tip position sensor unit 52. Here, the chip tip position sensor unit 52 includes a photoelectric sensor light emitting unit 36 and a photoelectric sensor light receiving unit 37, and the tip of the chip 72 is detected by the sensor light 35. In addition to the photoelectric sensor, any chip tip position sensor unit 52 may be used as long as its tip can be detected. The chip 78 whose tip is detected by the chip tip position sensor unit 52 is positioned on the DNA reaction vessel 67 by further rotating the nozzle transfer arm 68. The DNA reaction vessel wheel 66 on which the DNA reaction vessel 67 is mounted is rotatable about the DNA reaction vessel wheel rotation axis 69, and the desired DNA reaction vessel 67 is positioned under the objective lens (not shown) of the microscope 65. As described above, according to the embodiment of the present invention, the user can obtain the analysis result only by setting the reagent pack and the reaction vessel in the analyzer. Can be done. Furthermore, since the cleaning up after the analysis is only the disposal of the reagent pack and the reaction vessel, it is extremely easy to handle and operate.

By collecting various types of reagents, cleaning solutions, chips, sample cups, etc. for each analysis and packing them, it is possible to reduce the management of complicated chemicals and consumables. Furthermore, since the solution type and concentration are preset, mistakes do not occur. Further, by automating the operation by the barcode information (including the information that can be read by a computer other than the barcode), the burden on the operator is reduced and the operation error due to the input error or the like is eliminated. In addition, it is possible to further prevent mistakes by making it possible to confirm the inspection type and the like by displaying the barcode information.

By combining the reagent pack and the reaction vessel into one set, all consumables involved in the analysis can be packaged in one package. Therefore, the supply source of consumables can be unified, and quality control and manufacturing responsibility can be unified. In addition, the supplier can collectively manage the supply of all consumables including the analyzer, which facilitates quality control. Furthermore, since the consumables are included in one set, it is easy to reduce the size and price of the analyzer.

In the above embodiments, the case of detecting DNA has been described as an example, but it can also be applied to the case of detecting proteins, antigen antibodies, and other biochemical reactions.

The present invention is not limited to each of the above embodiments, and it is possible to carry out various modifications at the implementation stage without departing from the gist thereof. Further, each of the above embodiments includes inventions at various stages, and various inventions can be extracted by an appropriate combination of a plurality of disclosed constituent requirements.

Further, for example, even if some constituent requirements are deleted from all the constituent requirements shown in each embodiment, the problems described in the column of problems to be solved by the invention can be solved, and the effects described in the effect of the invention can be solved. If is obtained, a configuration in which this configuration requirement is deleted can be extracted as an invention.

<figref num="1">The schematic system block diagram of the analyzer which concerns on one Embodiment of this invention.</figref><figref num="2">The whole view of the analyzer which concerns on one Embodiment of this invention.</figref><figref num="3">The schematic block diagram of the reagent pack which concerns on one Embodiment of this invention.</figref><figref num="4">The figure for demonstrating the dispensing operation.</figref><figref num="5">The figure which shows the schematic structure of the DNA reaction vessel.</figref><figref num="6">The whole view of the analyzer which concerns on other embodiment of this invention.</figref><figref num="7">The schematic block diagram of the reagent pack which concerns on other embodiment of this invention.</figref>

Code description

1 ... Syringe pump, 2 ... Syringe, 4 ... X-axis linear motion robot, 5 ... Reagent pack, 6 ... Chip, 7 ... Solution, 8 ... Sample container, 9 ... Sample, 10 ... Chip tip position sensor holding plate, 11 ... Chip tip position sensor, 12 ... Chip removal unit, 13 ... Waste liquid container, 14 ... Sample stand, 15 ... DNA reaction vessel, 16 ... sample tank, 17 ... solution (sample), 18 ... microscope translucent tube, 19 ... chip, 20 ... nozzle, 21 ... Z-axis Linear robot, 22 ... timing belt, 23, 24 ... timing pulley, 25 ... vent pipe, 26 ... analyzer, 27 ... Z-axis driver controller, 28 ... Y-axis driver Controller, 29 ... chip tip position sensor amplifier, 30 ... X-axis driver controller, 31 ... computer (control), 32 ... pointing device, 33 ... display, 34 ... keyboard, 35 ... sensor light, 36 ... photoelectric sensor light emitting part, 37 ... photoelectric sensor light receiving part, 38 ... light source, 39 ... CCD camera, 40 ... objective lens, 41 ... sample Linear robot for table transfer, 42 ... Solution storage lid film, 43 ... Reagent pack cover, 44 ... Bar code label, 45 ... Positioning hole, 46 ... Sample table transfer driver controller, 47 ... Syringe driver controller, 48 ~ 55 ... Chip container, 56 ~ 63 ... Solution storage, 64 ... Sample pretreatment container, 65 ... Microscope, 66 ... DNA reaction Container Wheel, 67 ... DNA Reaction Vessel, 68 ... Nozzle Transfer Arm, 69 ... DNA Reaction Vessel Wheel Rotating Shaft, 71 ... Reagent Pack Wheel, 72 ... Nozzle, 73 ... Reagent Pack, 74 ... Positioning Pin, 75 ... Syringe Pack Wheel Rotating Shaft, 77 ... Syringe Pack Support, 79 ... Sample Container, 78, 81-86, 101 ... Chip Container, 87 ...Nozzle transfer arm rotation shaft, 80, 88 to 91, 93, 94, 99 ... Solution storage, 92 ... Solution storage lid film, 95 ... Waste liquid container, 96 ... Sample pretreatment Container section, 97 ... bar code label, 98 ... positioning hole and / or notch, 100 ... reagent pack cover, 104 ... reagent pack, 105, 106 ... positioning pin, 107 .. .Y-axis linear motion robot, 108 ... reagent pack mounting part, 109 ... reaction vessel, 111 ... DNA reaction vessel sample tank, 112 ... DNA probe, 113 ... electrical part, 300. .. Chip tip position sensor unit.