Kluyveromyces host cells and their use for expressing polypeptides

Kluyveromyces host cells and their use for expressing polypeptides _ _

GIST-BROCADES N.V.

C: 75356

Technical Field

This invention relates to methods for preparing and using Kluweromvces for the production of polypeptides of 1 interest which are secreted into the growth medium. The invention is exemplified by sequences useful in the production of chymosin and precursors thereof, tissue plasminogen activator (t-PA), and human serum albumin (HSA), in Kluweromvces.

Background of the Invention

The bright promise of production of peptides in microorganisms has been tarnished by a number of factors. In many instances, where the peptide has been produced and 20 retained in the cytoplasm, inclusion bodies have resulted requiring denaturation and renaturation of the protein, frequently with only partial or little success. In other instances, the peptide has been subjected to substantial degradation, so that not only are yields low, but also com25 plicated mixtures are obtained which are difficult to separate. As a potential solution to these difficulties, the possibility of secretion of the desired peptide into the nutrient medium has been investigated. Secretion has met with limited success, since not all proteins have been found 30 to be capable of secretion in the host which have been employed. Even when secreted, the processing of the peptide may result in a product which differs from the composition and/or conformation of the desired peptide. There is, therefore, substantial interest in being able to develop systems 35 for the efficient and economic production of active peptides under conditions which allow for the use of the peptides in a wide variety of environments', both in vitro and in vivo.

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RELEVANT LITERATURE

European Patent Application (EPA) 0096430 discloses

Kluyveromvces as a host for cloning and expression of foreign genes. However, no mention was made of the capability of secreting proteins into the growth medium.

The leader sequence of amyloglucosidase for Asperqillus is described by Boyle et al., EMBO J. (1984) 3.:1581-1585 and Innis et al., Science (1985) 228:21-26. Lactase promoters are described by Bruenig et al. Nucleic Acids Res. (1984) 12:2327-2341. The use of signal peptides associated with mating-type a-factor and of the enzymes invertase and acid phosphatase to direct the secretion of heterologous proteins in Saccharomvces has been described in EP-A-0123544 and by Smith et al._f Science (1985) 229:1219.

Production of preprochymosin, prochymosin and chymosin in Saccharomvces has been studied by Mellor et al., Gene (1983) 24:1-14. When prochymosin is made intracellularly in Saccharomvces, only a low percentage of the prochymosin obtained is activatable. See Moir et al. in Developments in Industrial Biology (1985) 26:75-85; Mellor et al., Gene (1983) 24:1-14; Kingsman et al. in Biotechnology and Genetic Engineering Reviews Vol. 3 (1985) 376-418. The aggregated prochymosin produced by Saccharomvces required complicated methods of denaturation and renaturation to solubilize the prochymosin. See WO 83/04418 and EP-A-0114506.

SUMMARY OF THE INVENTION

The present invention concerns a method for producing a polypeptide of interest in a KIuyveromyces host cell, said method comprising:

introducing into said host cell an expression cassette which comprises, in the direction of transcription, a transcriptional initiation regulatory region functional in said host cell, a signal sequence functional in said host cell joined in reading frame with a DNA

2a 87258/1 sequence encoding said polypeptide of interest, and a transcriptional termination regulatory region functional in said host cell, growing said host cell comprising said expression cassette in a culture medium whereby said polypeptide of interest, or part thereof, is secreted into the culture medium.

The present invention further comprises Kluyveromyces host cells for use in the above method as well as DNA constructs comprising:

in the direction of transcription, a transcriptional initiation regulatory region functional in said host cell, a signal sequence functional in said host cell joined in reading frame with a DNA sequence encoding said polypeptide of interest, and a transcriptional termination regulatory region functional in said host cell, growing said host cell comprising said expression cassette in a culture medium whereby said polypeptide of interest, or part thereof, is secreted into the culture medium.

BRIEF DESCRIPTION OF THE FIGURES

Figure 1 is a diagram of the plasmid pGBTe418;

Figure 2 is a diagram of the plasmid pGB901;

Figure 3 is a description of the synthesized oligonucleotides for the signal sequence adapted, from the amyloglucosidase signal sequence;

Figure 4 is a description of a synthetic signal sequence;

Figure 5 is an immunoblot showing the secretion of prochymosin by K. lactis;

Figure 6 is the sequence of the entire BamHl insert from pDMIOOPC comprising the fusion peptide of the a-factor of £. cerevisiae and prochymosin and transcriptional regulatory regions;

Figure 7 is a restriction map of plasmid pKS105;

Figure 8 shows the strategy used to design oligonucleotide probes used to identify K. lactis a-factor DNA;

Figure 9 is the complete sequence of a DNA fragment encoding the K. lactis a-factor;

Figure 10 is a description of plasmids employed for expression of the fusion of the a-factor signal sequence and the prochymosin structural gene;

Figure 11 shows the sequences around the junctions in a-factor/prochymosin fusions;

Figure 12 is the sequence of the BamHI/Sall insert of pAB309;

Figure 13 represents the sequences of the primers for mutagenesis of K. lactis a-factor leader DNA;

Figure 14 is a diagram of the plasmid pUCG418;

Figure 15 is a diagram of the plasmid pGBtPAl;

Figure 16 shows the secretion of human t-PA by K. lactis as analysed on a SDS-polyacrylamide gel overlayered with a plasminogen/fibrin-agarose gel;

Figure 17 is a diagram of the plasmid pGBtPA2;

Figure 18 is a diagram of the plasmid pGBHSA3;

Figure 19 shows the secretion of HSA by K. lactis as analysed on a 10% polyacrylamide gel.

DESCRIPTION OF THE SPECIFIC EMBODIMENTS

In accordance with the subject invention, expression cassettes are provided which allow for the efficient and economic production of polypeptides by Kluyveromyces yeast 10 cells. The expression cassettes have transcriptional and translational regulatory sequences functional in a Kluweromvces host cell and an open reading frame coding for a peptide of interest under the transcriptional and translational control of the regulatory regions. The open reading frame 15 also may include a leader sequence recognized by the Kluweromvces host which provides for secretion of the polypeptide into the grqwth medium. The Kluweromvces cells used may be either laboratory or industrial strains.

The expression cassette will include in the 5'-3' 20 direction of transcription, a transcriptional and translational initiation regulatory region, an open reading frame encoding a peptide of interest, desirably having a signal sequence for secretion recognized by Kluweromvces. and a translational termination region. The expression cassette 25 will further comprise a transcriptional termination regulatory region. The initiation and termination regulatory regions are functional in Kluweromvces and provide for efficient expression of the peptide of interest without undesirable effects on the viability and proliferation of 30 the Kluweromvces host.

The transcriptional and translational initiation regulatory region may be homologous or heterologous to Kluweromvces Of particular interest are transcriptional initiation regions from genes which are present in Kluyvero35 mvces or other yeast species, such as Saccharomvces. for example, cerevisiae, Schizosaccharomvces. Candida, etc., or other fungi, for example, filamentous fungi such as Asperaillus. Neurospora, Penicillium. etc. The transcriptional initiation regulatory regions may be obtained for example from genes in the glycolytic pathway, such as alcohol dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglucoisomerase, phosphoglycerate kinase, etc., or regul5 atable genes such as acid phosphatase, lactase, glucoamylase, etc.

Any one of a number of regulatory sequences may be preferred in a particular situation, depending upon whether constitutive or induced transcription is desired, the parti10 cular efficiency of the promoter in conjunction with the open reading frame of interest, the ability to join a strong promoter with a control region from a different promoter which allows for inducible transcription, ease of construetion, and the like. These regulatory regions find ample 15 precedent in the literature. See, for example, EP-A-0164566, incorporated herein by reference.

Secretion of heterologous proteins in genetically modified microorganisms is generally accomplished by one of two methods. In the first, the leader sequence is homologous 20 to the protein; in the second, the leader sequence is homologous to the host organism. Other alternatives of particular interest in the present invention for secretion of heterologous proteins in Kluyveromyces include the use of a leader sequence heterologous to both Kluyveromyces and the 25 peptide of interest, or a synthetic leader sequence specifically designed. The DNA encoding the former leader sequence can be obtained by isolation or made synthetically. Thus, the open reading frame usually will include a wild-type or mutated gene, where the signal sequence is normally associa30 ted with the remainder of the coding sequence, a hybrid or chimeric open reading frame, where the signal sequence is normally not associated with the remaining portion of the open reading frame, or a synthetic sequence, where the signal sequence and the remainder of the open reading frame 35 are synthesized to provide for preferred codons, convenient restriction sites, novel amino acid sequences, and the like, or combinations thereof. Signal sequences which may be employed may be obtained from genes, such as a-factor, invertase, amyl©glucosidase, native or wild type signal sequences present in structural genes and recognized by

Kluweromvces, Saccharomvces. other fungi, e.g. Neurospora,

Aspergillus, and other eukaryotes.

Of particular interest is the use of a signal sequence which provides for secretion of the peptide of interest into the nutrient medium, rather than into the periplasmic space. For the most part, the signal sequence will be the 5’-terminus of the open reading frame. However, in some 10 situations, it may be desirable to have the signal sequence internal to the open reading frame. For use of internal signal sequences for secretion, see U.S. Patent No. 4,338,397 and Perara and Lingappa, J. Cell Biology (1985) 101:2292-2301. Genes into which the open reading frame of 15 interest may be inserted include highly expressed constitutive genes, for' example, genes encoding enzymes of the glycolytic pathway, or highly expressed regulatable genes such as lactase, amyloglucosidase, or the like.

For optimal gene expression, the nucleotide sequences 20 surrounding the translational initiation codon ATG have been found to be important in yeast cells and in animal cells. For example, M. Kozak, Microbiol. Revs. (1983) £7:1-45 has studied extensively the effect of these regions on the expression of insulin in COS ceils. Similarly, specific 25 nucleotides are found more frequently in highly expressed yeast proteins than others indicating the important effect of these nucleotides on the level of expression of these genes.

For optimal gene expression of exogenous genes it 30 will be important to modify the nucleotide sequences surrounding the initiation codon ATG. This can be done by sitedirected mutagenesis or by fusing the exogenous gene in frame to an endogenous Kluweromvces gene, preferably a highly expressed gene, such as the lactase gene.

Normally, it will be desirable to provide that the signal leader is cleaved from the peptide of interest during the secretory process, rather than subsequent to the secretory process, although either procedure may find use. Usual7 ly, the processing signal employed will be the processing signal naturally occurring with the signal sequence or a processing signal which has been modified from the naturally occurring one, which is still effective for providing for a 5 peptide signal resulting in hydrolytic cleavage of the signal peptide and processing signal peptide from the peptide of interest. Various processing signals have been sequenced and defined, such as a-factor (see for example U.S. Patent No. 4,546,082, which is incorporated herein by 10 reference), amyloglucosidase, a-amylase, etc. In some instances, other peptidase-recognized sequences may be employed which may required subsequent cleavage for isolation of the desired peptide. These sequences include dibasic peptides, e.g. KR, (θ)₄^κ׳ ^{and EA}׳ which are cleaved by KEX2, 15 bovine :enterokinase, and a yeast membrane peptidase, respectively.

The peptide of interest may be native to the host or heterologous, being derived from prokaryotic or eukaryotic sources, which eukaryotic sources may involve fungi, pro20 tists, vertebrates, non-vertebrates, and the like. The peptide products may include enzymes, such as lactase, aamylase, /?-amylase, amyloglucosidase, chymosin, etc., mammalian peptides, such as hormones, interleukins, cytokines, cachexin, growth factors, e.g. platelet derived, epidermal, 25 skeletal, etc., growth hormone, follicle stimulating hormone, interferons (a-, -0-,. and ^-), blood factors such as factor V, VI, VII, VIII (vW or c), IX, X, XI or XII, plasminogen activator (tissue or urinary), serum albumin, e.g. human serum albumin, colony growth factor (e.g. GM), 30 erythropoietin, thaumatin, insulin, etc.

These structural genes may be obtained in a variety of ways. Where the amino acid sequence is known, the structural gene may be synthesized in whole or in part, particularly where it is desirable to provide yeast-preferred 35 codons. Thus, all or a portion of the open reading frame may be synthesized using codons preferred by Kluweromvces. Preferred codons may be determined by those codons which are found in the proteins produced in greatest amount by the

Kluweromvces host e.g. glycolytic enzymes. Methods for synthesizing sequences and bringing the sequences together are well established in the literature. Where a portion of the open reading frame is synthesized, and a portion is 5 derived from natural sources, the synthesized portion may serve as a bridge between two naturally occurring portions, or may provide a 3'-terminus or a 5'-terminus. Particularly where the signal sequence and the open reading frame encoding the peptide are derived from different genes, synthe10 tic adaptors commonly will be employed. In other instances, linkers: may be employed, where the various fragments may be inserted at different restriction sites or substituted for a sequence in the linker.

For the most part, some or all of the open reading 15 frame will be from a natural source. Methods for identifying sequences of interest have found extensive exemplification in the literature, although in individual situations, different degrees of difficulty may be encountered. Various techniques involve the use of probes, where at least a 20 portion of the naturally occurring amino acid sequence is known, where genomic or cDNA libraries may be searched for complementary sequences. Alternatively, differential transcription can be detected when the gene of interest can be induced or when cells are from the same host but of dif25 ferent differentiation, by comparing the messenger RNA’s produced, other techniques have also been exemplified.

The termination region may be derived from the 3'region of the gene from which the initiation region was obtained or from a different gene. A large number of termina30 tion regions are known and have been found to be satisfactory in a variety of hosts from the same and different genera and species. The termination region is usually selected more as a matter of convenience rather than because of any particular property. Preferably, the termination region will be 35 derived from a yeast gene, particularly Saccharomvces or Kluweromvces.

In developing the expression cassette, the various fragments comprising the regulatory regions and open reading frame may be subjected to different processing conditions, such as ligation, restriction, resection, in vitro mutagenesis, primer repair, use of linkers and adaptors, and the like. Thus, nucleotide transitions, transversions, insert5 ions, deletions, or the like, may be performed on the DNA which is employed in the regulatory regions and/or open reading frame.

During the construction of the expression cassette, the various fragments of the DNA will usually be cloned in 10 an appropriate cloning vector, which allows for expansion of the DNA, modification of the DNA or manipulation by joining or removing of the sequences, linkers, or the like. Normally, the vectors will be capable of replication in at least a relatively high copy number in E. coli. A number of vectors 15 are readily available for cloning, including such vectors as pBR322, pACYC184, pUC7-19, M13, Charon 4A, and the like.

The cloning vectors are characterized by having an efficient replication system functional in E. coli. Also, the cloning vector will have at least one unique restriction 20 site, usually a plurality of unique restriction sites and may also include multiple restriction sites, particularly two of the same restriction sites for substitution. In addition, the cloning vector will have one or more markers which provide for selection for transformants. The markers 25 will normally provide for resistance to cytotoxic agents such as antibiotics, heavy metals, toxins or the like, compiementation of an auxotrophic host, or immunity to a phage. By appropriate restriction of the vector and cassette, and, as appropriate, modification of the ends, by chewing back or 30 filling in overhangs, to provide for blunt ends, by addition of linkers, by tailing, complementary ends can be provided for ligahion and joining of the vector to the expression cassette or component thereof.

After each manipulation of the DNA in the development 35 of the cassette, the plasmid will be cloned and isolated and, as required, the particular cassette component analyzed as to its sequence to ensure that the proper sequence has been obtained. Depending upon the nature of the manipul/ - 10 ation, the desired sequence may be excised from the plasmid and introduced into a different vector or the plasmid may be restricted and the expression cassette component manipulated, as appropriate.

In some instances a shuttle vector will be employed where the vector is capable of replication in different hosts requiring different replication systems. This may or may not require additional markers which are functional in the two hosts. Where such markers are required, these can be 10 included in the vector, where the plasmid containing the cassette, the two replication systems, and the marker(s) may be transferred from one host to another, as required. In the present situation, the second replication system would be a replication system functional in Kluyveromyces. The replica15 tion systems which may be used may be derived from plasmids, e.g. pKDl from Kluyveromyces drosophilarum, viruses, or the chromosome of Kluyveromyces or other species, particularly one associated with Kluyveromyces, such as Saccharomvces. Thus, replication systems include the replication system of 20 the 2 micron plasmid found in Saccharomvces and an autonomously replicating sequence (ARS) gene, for example when used in conjunction with a centromere sequence, or the like. If desired, regions of homology may be provided to encourage integration of the expression cassette into the genome of 25 the Kluyveromyces host.

Of particular interest in the constructs of the subject invention is a sequence derived from Kluyveromyces DNA chromosomes referred to as KARS, which provide for high transformation frequency. The KARS gene may be obtained by 30 screening a library of Kluyveromyces DNA fragments for enhanced transformation efficiency. In this manner, fragments can be obtained which contain KARS sequences, which fragments can be further modified by restriction, resection, or primer repair, to provide a fragment of approximately 200 bp 35 and not more than about 5000 bp, more usually from about 200 bp to 2000 bp which provides for enhanced transformation efficiency. The presence of the KARS gene can provide transformation of g. lactis auxotrophic species to prototophy at a frequency of at least about 10 per microgram of DNA, usually at a frequency of 10 per microgram of DNA or higher.

The manner of transformation of E. coli with the 5 various DNA constructs (plasmids and viruses) for cloning is not critical to this invention. Conjugation, transduction, transfection or transformation, e.g. calcium chloride or phosphate mediated transformation, may be employed. By contrast, for yeast, for the most part the prior art has relied 10 on transformation of protoplasts employing combinations of calcium ions and polyethylene glycol of from about 2000 to 8000, usually 4000 to 7000 daltons.

An alternative method of transformation involves growing in a standard yeast nutrient medium to a density of 1 to 25, desirably 4 to 10 Οϋ₆₁θ. The Kluweromyces cells are then harvested, washed and pretreated with chaotropic ions, particularly the alkali metal ions lithium, cesium or rubidium, particularly as the chloride or sulfate, more particularly the lithium salts, at concentrations of 20 about 2 mM to IM, preferably about 0.1 M. After incubating the cells with the chaotropic ion(s), the DNA is added and the incubation is prolonged for a short period of time at a moderate temperature, generally from about 20’C to 35°C, for about 5 to 60 min. Then polyethylene glycol is added, desir25 ably at a concentration to about 25 to 50%, where the entire medium may be diluted by adding an equal volume of a polyethylene glycol concentrate to result in the desired final concentration. The polyethylene glycol will be of from about 2000 to 8000 daltons, preferably about 4000 to 7000 daltons. 30 Incubation will generally be for a relatively short time, generally from about 5 to 60 min. Desirably, the incubation medium is subjected to a heat treatment of from about 1 to 10 min. at about 35°C to 45’C, preferably about 42’C.

For selection, any useful marker may be used, al35 though the number of markers useful with Kluyveromyces is narrower than the markers used for Saccharomvces. Desirably, resistance to kanamycin and the aminoglycoside G418 are of interest, as well as complementation of a gene in the tryptophan metabolic pathway, particularly TRP1 or in the lactose metabolic pathway, particularly LAC4.

Although a marker for selection is highly desirable 5 for convenience, other procedures for screening transformed cells have been described. See for example G. Reipen et al. Current Genetics (1982) 5:189-193. Besides the use of an indicator enzyme such as 0-lactamase, transformed cells may be screened by the specific products they make. In the case 10 of chymosin, for example, synthesis of the product may be determined by an immunological or an enzymatic method.

The vector used may be capable of extrachromosomal maintenance in Kluweromvces or result in integration into the Kluweromvces gene. It has been found that the 2 micron 15 plasmid replication system from Saccharomvces provides for extrachromosomal maintenance in Kluweromvces. In addition, one may use a combination of a centromere, such as the Saccharomyces CEN3 and a high transformation frequency sequence, such as ARS or KARS. If selective maintenance is pro20 vided, such as complementation or resistance to an antibiotic to which Kluweromvces is susceptible, the ARS-like sequences will usually suffice for extrachromosomal maintenance.

For large scale fermentation even a small loss of 25 plasmid stability will greatly affect the final yield of the desired protein. To increase the stability of recombinant molecules in host cells, for example Kluyveromyces, integration of the recombinant molecules into the host chromosome may be used.

Where integration is desired, it will usually be desirable to have a sequence homologous to a sequence of the chromosome of the host, so that homologous recombination may occur. It is understood, that random integration also occurs, so that the homologous sequence is optional. Where 35 an homologous sequence is employed, the homologous sequence will usually be at least about 200 bp and may be 1000 bp or more. In addition, where integration is involved, one may wish to haye amplification of the structural gene. Amplifi13 cation has been achieved by providing for a gene in tandem with the desired structural gene, which provides for selective advantage for the host in a selective medium. Thus, the genes expressing dihydrofolate reductase, metallothioneins, 5 thymidine kinase, etc., have proven useful in a variety of hosts to provide for amplification, where the gene provides protection from a toxin, such as methotrexate, heavy metals, such as copper and mercury, and the like.

Vectors of interest providing for stable replication :10 include KARS vectors originating from K. lactis, e.g. PKARS12 and pKARS2, which plasmids comprise a K. lactis DNA fragment containing the KARS12 or KARS 2 sequence in the S. cerevisiae plasmid YRp7. A vector employed for integration is, for example, pL4, a hybrid plasmid of the ARS1 carrying 15 plasmid YRp7 and g. lactis Xhol DNA fragment carrying the LAC4 gene. See EP-A 0096430.

Plasmids of particular interest include plasmids having the 2 micron plasmid replication system, the LAC4 gene, the Tn601 and Tn5 kanamycin resistance gene, which 20 also provides resistance to the antibiotic G418 in Kluweromyces (Jimenez and Davis, Nature (1980) 287:869-871). This plasmid provides for autonomous replication in Kluweromvces and can be selected for by resistance to G418 on regeneration plates containing glucose, sorbitol, and 0.2 jug/ml G418, 25 while avoiding elevated concentrations of KC1, which interferes with the sensitivity of Kluweromvces to G418. Preferred plasmids include the TRP1 gene, particularly from S. cerevisiae, the LAC4 gene, particularly from K. lactis, the Kan gene providing for resistance against antibiotic G418 30 from Tn5, or the like.

The subject vectors and constructs are introduced into an appropriate host for cloning and expression of the desired structural genes. After transformation, colonies will normally appear on regeneration medium within about 5 35 to 6 days. Where an antibiotic is employed for selection, the colonies should be screened to ensure the absence of spontaneous mutation to a resistant strain. Employing the plasmids and the methods of the subject invention, about 5% of resistant colonies were found to contain the plasmid construct providing for at least about 4 transformants per Mg of plasmid DNA. Where selection was based on the presence of the LAC4 gene, using plates containing lactose as the 5 sole carbon source and 0.6M KC1 as an osmotic stabilizer, all of the surviving colonies were found to be transformants and not spontaneous revertants. About 20 transformants were obtained after about 4 to 5 days of incubation at moderate temperature, e.g. 30’C.

As a host organism, Kluyveromyces is especially suitable for the production of heterologous proteins, for example for the production and extraction of the enzyme chymosin and its precursors preprochymosin, pseudochymosin and prochymosin, for human serum albumin (HSA), tissue plas15 minogen activator (t-PA), and thaumatin and its precursor forms. Although other organisms such as Saccharomvces produce prochymosin in reasonable amounts, the produced prochymosin cannot be extracted in an activq or activatable form. We have surprisingly found that more than 90% of the 20 total amount of the prochymosin produced by Kluweromvces can be extracted in an active form with very simple standard techniques.

Any of the many Kluweromvces species may be employed. Either laboratory or industrial, preferably industrial, 25 strains may be used. By industrial species is intended, Kluweromvces strains from organisms which may be isolated from natural sources or may be available from depositories or other sources or obtained by modification, e.g. mutation, of such strains. The industrial strains are characterized by 30 being resistant to genetic exchange, being prototrophic or made prototrophic by a single gene being introduced into the host strain, and are usually selected for improved production of peptides. Among the Kluyveromyces species which may find use are K. lactis, K. fraqilis, K. bulqaricus, K.

thermotolerans. K. marxianus. etc. It should be further noted that the Kluyveromyces organisms are on the GRAS (Generally Recognized As Safe) list. Their use for production of products to be used in vivo or to be ingested normally will not require special governmental review and approval.

Both wild type and mutant Kluyveromyces. particularly Kluyveromyces lactis or Kluyveromyces fraqilis may be 5 employed as hosts. Hosts of particular interest include K. lactis SD11 lac4 trpl and K. lactis SD69 lac4, and the wildtype strain CBS 2360 (see EP-A-0096430).

For maintaining selective pressure on the transformants for maintenance of the plasmids, selective media 10 may be used, such as a yeast nitrogen-based medium, 2% lactose instead of glucose for K. lactis SD69 lac4 (PTY75-LAC4) and for K. lactis SD69 lac4 (pL4) and a yeast nitrogen-based medium (Difco) plus 2% glucose for K. lactis SD11 lac4 trpl (pKARS12). See for the transformants mentioned, EP-A15 0096430. Similarly, strains containing plasmids conferring antibiotic resistance, for example against G418, may be cultivated in a medium containing said antibiotic.

Where the hybrid plasmids are employed for large scale production of the desired protein, it would generally 20 be useful to remove at least substantially all of the bacterial DNA sequences from the hybrid plasmids.

Depending upon the nature of the structural gene of interest, the expression product may remain in the cytoplasm of the host cell or be secreted. It has been found that not 25 only the proteins that remain in the cell but also those that are secreted are soluble. Where the expression product is to remain in the host cell, it may generally be desirable to have an inducible transcription initiation region, so that until the transformant has reached a high density, 30 there is little or no expression of the desired product. After sufficient time for the expression product to form, the cells may be isolated by conventional means, e.g. centrifugation, lysed and the product of interest isolated. Depending upon the nature and use of the product, the lysate 35 may be subjected to various purification methods, such as chromatography, electrophoresis, solvent extraction, crystallization, or the like. The degree of purity may vary from about 50%, to 90% or higher, up to essential purity.

When the product is to be secreted, both constitutive and non-constitutive transcriptional initiation regions may be employed, depending on the fermentation process used for the production of the protein or polypeptide of interest.

The expression product may be secreted into the culture medium, and produced on a continuous basis, where the medium is partially withdrawn, the desired product extracted, e.g., by affinity chromatography, or the like, and the spent medium discarded or recirculated by restoring essential 10 components.

All publications and patent applications mentioned in this specification are indicative of the level of skill of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorpo15 rated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

The following examples are offered by way of illustration and not by limitation.

EXPERIMENTAL

Example

Construction of chymosin expression plasmids containing a long lactase promoter sequence

A. Construction of pUCla56

Chromosomal DNA was isolated from Kluyveromyces lac30 tis strain CBS 2360 (Das and Hollenberg, Current Genetics (1982) 5:123-128), cleaved with Xhol. and separated according to size on a sucrose gradient. Fractions containing the lactase gene were detected with a LAC4 probe from plasmid pK16 after spotting the DNA on a nitrocellulose filter (see 35 EP-A-0096430, Example 16.C2). DNA containing the LAC4 gene was cloned into the Sall site of plasmid pPA153-215 (Andreoli, Mol. Gen. Genet. (1985) 199:372-380) giving rise to plasmid pPA31. An Xbal fragment of pPA31 containing the lactase gene was subcloned in the Xbal site of pUC19 (Yanisch-Perron et al., Gene (1985) 33:103-119) which yields plasmid pUCla56.

B. Introduction of the G418 resistance gene in the terminator of the lactase gene The terminator fragment containing the G418 resistance marker was obtained from plasmid pGBTeG418. E. coli containing pGBTeG418 was deposited with Centraal Bureau voor

Schimmelcultures under number CBS 184.87 on February 26, 1987. Plasmid pGBTeG418 (see Figure 1) consists of the plasmid pPA153-215, as described above, and a 5.6 kb fragment consisting of the 3.7 kb BamHI K. lactis lactase terminator fragment (Breuning et al. Nucl. Acid Res. (1984) 12:232715 2341) and the Tn5 gene (Reiss et al. EMBO J. (1984) 3:3317) conferring resistance to G418 under the direction of the promoter alcohol dehydrogenase I (ADH) from yeast, similar to that described by Bennetzen and Hall, J. Biol. Chem. (1982) 257:3018-3025.

C. Construction of plasmid pGB900 containing the G418 resistance gene and prochymosin encoding DNA

The 3.6 kb HindIII־XbaI fragment from plasmid pGBTeG418 containing the G418 resistance gene (see Example 25 IB) and the Sall-Hindlll fragment containing the prochymosin gene from pGB123 (see EP-A-0096430) were ligated in pUC19 cleaved with Sall and Xbal. This yielded plasmid pGB900.

D. Construction of plasmid pGB901 (see Figure 2)

Plasmid pGB901 was constructed by ligating the foliowing four fragments:

(1) a 3.6 kb Xbal-Haell fragment containing the lactase promoter to about position -90 from the lactase ATG start codon isolated from pUCla56, (2) a Haell-Sall fragment extending from the above

Haell site to a Sall site, which was ligated to position -26 in a similar Bal31 experiment as described in Example 16.C2 of EP-A-0096430. However, in this experiment only a Sall linker was used. This fragment has the following sequence:

S' TTAAC ACTTGAAATT TAGGAAAGAG CAGAATTTGG CAAAAAAAAT AAAAAAAAAA TAAACACG 3 3' CGCGAATTG TGAACTTTAA ATCCTTTCTC GTCTTAAACC GTTTTTTTTA TTTTTTTTTT ATTTGTGCAG CT S' (3) the 5.1 kb Sall-Xbal fragment containing prochymosin and G418 from pGB900 (see Example 1C), (4) pUC19 cleaved with Xbal.

During the construction of the plasmid the CG sequen10 ce from the Haell site was inadvertently removed, thereby creating a Hindlll site at this position.

Prochymosin-encoding DNA is present in plasmid pGB901. This may readily be converted to plasmids with preprochymosin, pseudochymosin or chymosin DNA by using the 15 Sall-BolII fragments from pGB 131, 122 or 124, respectively (see EP-A-0096430) .

Example

Secretion of prochymosin from Kluweromvces lactis

To direct the synthesis of prochymosin in Kluweromyces, plasmid pGB901 was used to transform K. lactis 25 strains SD11 and CBS 2360 with similar results. The transformation was carried out essentially as described in Examples 4 and 14 of EP-A-0096430, by using intact plasmid DNA or plasmid DNA cut with restriction endonucleases. In the latter case restriction endonucleases were used which 30 cut in the promoter region, e.g., SacII. Ndel. SnaBI or Spel. or in the terminator region, e.g., EcoRV. or both the promoter and terminator regions.

K. lactis strain CBS 2360 was grown in 100 ml of YEPD-medium (1% yeast extract, 2% peptone, 2% glucose) 35 containing 2.5 ml of a 6.7% yeast nitrogen base (Difco) solution to an ODg^o ^of about 7. The cells were collected by centrifugation from 10 ml of the culture, washed with TEbuffer (10 mM Tris-HCl pH 7.5, 0.1 mM EDTA) and resuspended in 1 ml TE-buffer. An equal volume of 0.2 M lithium acetate was added and the mixture was incubated for 1 hr at 30°C in a shaking waterbath. Plasmid pGB901 (15 pg) was cut at the unique SacII site in the lactase promoter, ethanol precipitated and resuspended in 15 μΐ TE-buffer. This DNA preparation was added to 100 μΐ of the pre-incubated cells and the incubation was prolonged for 30 minutes. Then an equal volume of 70% PEG 4000 was added and the mixture was incubated for 1 hr at the same temperature, followed by a heatshock of 5 minutes at 42’C. Then 1 ml of YEPD-medium was added and the cells were incubated for 1.5 hrs in a shaking waterbath of 30°C. Finally the cells were collected by centrifugation, resuspended in 300 μΐ YEPD and spread on agar plates containing 15 ml of YEPD agar with 300 pg/ml of G418, overlayered (1 hr before use) with 15 ml YEPD-agar without G418. Colonies were grown for 3 days at 30°C. K. lactis strain SD11 was transformed in a similar way, only the initial G418 concentration in the selection plates was lowered to 150 pg/ml. In one of the experiments transformants of CBS 2360 were grown at 30’C in YEP-medium containing 2% galactose. After 60 hours, cells and medium were separated by centrifugation. Cells were disrupted by treatment with glass beads. Culture medium and cell extract were treated at pH 2 before assaying for chymosin activity (see Foltman, Methods in Enzymology (1970) 19:421-426).

Cells were removed from cultures by centrifugation and the resulting supernatants were acidified to pH 2 by the addition of 1 M H^SO, and incubated for 2 hours at room temperature. The solutions were then neutralized to pH 6 by the addition of 2 M Tris base. A 50 μΐ volume of an appropriate dilution was added to a suspension of 12% non-fat dry milk in 10 mM CaCl₂ and incubated at 37״C until a clot formed. A unit of chymosin activity is defined as the amount of active chymosin required to produce a clot in 10 min. under these conditions. The supernatant contained milk-clotting activity due to the production and secretion of prochymosin by K. lactis transformants although no signal sequence for protein secretion was added to prochymosin. About 30-60% of the total prochymosin produced was found in the medium as determined by the above-described milk-clotting assay. Similar results were obtained when K. lactis strain SD11 was used.

Example 3

Lactase-chvmosin fusion proteins giving enhanced chymosin production

By taking various SnaBI-Sall fragments (from a Bal31 experiment similar to the one described in Example 16.C2 of EP-A-0096430 but using a single Sall linker only) variants of pGB901 containing a fusion between the lactase and chymosin proteins were obtained (Table 1). The extra amino acids provided by lactase DNA and linker sequences can be removed, along with the pro sequence of prochymosin, by treatment with acid. It was observed that a fusion containing 4 amino acids from the lactase coding sequence (pGB902) resulted in enhanced chymosin production.

Table

Nucleotide sequence at the junction between the lactase promoter and prochymosin in pGB901 and PGB902

PGB901 lactase promoter prochymosin

..TAAACACGTCGACGAATTC ATG GCTGAG

<img file="IL87258A_D0001.tif" />

Sall EcoRI

PGB902 lactase prochymosin

ATG TCTTGCCTCGTCGACGAATTCATGGCTGAG

I—^;—11—I

Sall EcoRI

Protein synthesis starts at the boxed ATG codon.

Example

Expression of preprochvmosin by Kluyveromyces transformants

The Sall site from the polylinker of pGB902 (see Example 3) was removed for convenience. pGB902 was partially digested with Sall, followed by a short incubation with 10 Bal31 (Boehringer). Linear fragments were isolated from an agarose gel, ligated and transformed into E. coli. A correct plasmid, pGB903, was obtained. Restriction analysis showed that this plasmid also has the Xbal and Hindi 11 sites removed from the polylinker.

To construct a plasmid containing and expressing preprochymosin, plasmid pGB903 was digested with the restriction endonucleases Sall and Bqlll. The 11 kb DNA fragment was isolated from an agarose gel by electroelution. Similarly, plasmid pGB124 containing the preprochymosin gene (see 20 EP-A-0096430, Example 16) was digested and the 0.3 kb SallBollI fragment containing the N-terminal part of the preprochymosin gene was isolated.

The 11 kb and the 0.3 kb DNA fragments were mixed, ligated with DNA ligase and transformed into E. coli. Plas25 mid pGB904 was isolated which contained the preprochymosin gene fused to a small part of the lactase gene (Table 2).

Table Nucleotide sequence at the junction between the lactase promoter and preorochvmosin in pGB904

PGB904 lactase preprochymosin

ATG TCTTGCCTCGTCGACGAATTCATG

Sall EcoRI 40

Protein synthesis starts at the boxed ATG codon.

K. lactis CBS 2360 cells were transformed with pGB904, which had been linearized with SacII. Transformants 5 were selected, grown and assayed for chymosin activity as described in Example 2. In the following Table 3 a comparison is made between the secretion of prochymosin from K. lactis CBS 2360 cells transformed with pGB902 (see Example

3) and with pGB9O4. (Pro)chymosin production is expressed in 10 arbitrary units per ml of cells at Οϋ_β1θ of 200.

Table

Secretion of prochymosin bv K. lactis cells transformed with PGB902 and pGB904 . _________________ _________________________________:----------------------------------------------------------

<td colspan="2"></td><td colspan="2"> pGB902</td><td colspan="2"> pGB904</td>

<td></td><td> Transformant</td><td colspan="2"> Supernatant Pellet</td><td colspan="2"> Supernatant Pellet</td>

<td></td><td> 1</td><td> 3.2</td><td> &lt;0.4</td><td> 22.4</td><td> 1.7</td>

<td></td><td> 2</td><td> .1.3</td><td> &lt;0.4</td><td> 33.3</td><td> 3.0</td>

<td> 20</td><td> 3</td><td> 7.1</td><td> 1.4</td><td> 28.0</td><td> 2.3</td>

<td></td><td> 4</td><td> 4.4</td><td> 0.66</td><td> 53.8</td><td> 5.8</td>

Example 5 25

Secretion of prochymosin bv Kluweromvces using heterologous leader sequences

A. Chemical synthesis of an amyloglucosidase leader seguence 30 and construction of a plasmid containing said leader seguence

The leader sequence of amyloglucosidase (AG) from Aspergillus awamori was published by Innis et al., Science (1985) 228:21-26. Based on the protein sequence, oligo35 nucleotides were derived to permit insertion of the leader sequence in front of the prochymosin gene (see Figure 3).

The oligonucleotides were synthesized with an Applied Biosystems DNA synthesizer. The oligonucleotides were purif23 ied by electrophoresis on a denaturing polyacrylamide gel, then electroeluted from the gel.

Plasmid pGB903 (see Example 4) was cut at the unique Sall site. The oligonucleotides were hybridized at 65’C, 5 50’C and 37°C for one hour each in 2xSSC. The oligonucleotides had no phosphate at the 5' end to prevent formation of multimers. The DNA was ligated into the Sall site using T4 polynucleotide ligase. The ligation mixture was transformed into E. coli HB101. Twenty-four of the colonies were cul10 tured and plasmid DNA isolated. One of the plasmids, pGB905, was shown to have the correct orientation of the oligonucleotides by restriction enzyme analysis. Plasmid pGB905 was transformed to K. lactis CBS 2360. (Pro)chymosin productioh was analyzed according to the procedure described 15 in Example 2. The results are of the (pro) chymosin production, in arbitrary units/ml of cells at OD_gl0 of 200, is shown in the following Table 4.

Table 4 20 Secretion of prochymosin by K. lactis cells transformed with PGB902 and PGB905

<td rowspan="2"> 25</td><td rowspan="2"> Transformant</td><td colspan="2"> pGB902</td><td colspan="2" rowspan="2"> pGB905 Supernatant Pellet</td>

<td> Supernatant</td><td> Pellet</td>

<td></td><td> 1.</td><td> 3.2</td><td> &lt;0.4</td><td> 60.6</td><td> &lt;0.4</td>

<td></td><td> 2.</td><td> 1.3</td><td> &lt;0.4</td><td> 56.4</td><td> &lt;0.4</td>

<td></td><td> 3.</td><td> 7.1</td><td> 1.4</td><td> 56.7</td><td> &lt;0.4</td>

<td> 30</td><td> 4. _.</td><td> 4.4</td><td> 0.66</td><td> 57.6</td><td> &lt;0.4</td>

B. Chemical synthesis of a novel synthetic leader sequence into construction of a plasmid containing the novel synthetic leader sequence.

A synthetic leader sequence was prepared which has a sequence different from any known leader sequence. Using this leader sequence, all prochymosin synthesized was secreted by Kluweromvces as shown below.

This synthetic leader sequence was devised using frequently occurring amino acids from position -6 to +2 of the signal sequence cleavage site (Von Heyne, Ear. J. Biochem. (1983) 133:17-21). Frequently occurring yeast codons 5 were also employed and extra nucleotides were incorporated in front of the ATG sequence to make up for the deletion of 26 nucleotides in pGB902. The oligonucleotides used and the resulting leader sequence are shown in Figure 5.

The synthetic leader sequence DNA was synthesized 10 using an Applied Biosystems DNA synthesizer. The resulting oligonucleotides were run on a 40 cm long, 1 mm thick polyacrylamide gel, containing TBE buffer (50 mM Tris, 50 mM borate, ImM EDTA, pH 8.3) and 7 M urea until the Bromophenol Blue marker had travelled 2/3 of the gel length. The DNA was

15. visualized, eluted from the gel and precipitated with ethanol.

Also from pGB901 a derivative was made with a : deletion around the Sall site resulting from the polylinker of pUC19. This was done by replacing the 0.5 kb SnaBI-Bqlll 20 fragment from pGB903 by the corresponding fragment from pGB901. The resulting plasmid was cut at the unique Sall site. The oligonucleotides were hybridized at 65’C, 50°c and 37°C for one hour each in 2xSSC. The DNA was ligated into the Sall site using T4 polynucleotide ligase. The plasmid 25 was then transformed into E. coli HB101. Of the colonies obtained, 24 were cultured and plasmid DNA isolated. One of the plasmids, pGB906, was shown to have the oligonucleotides in the correct orientation by restriction enzyme digestion. It was found that K, lactis CBS 2360 transformed with pGB906 30 secreted more than 95% of the prochymosin produced.

C. Analysis of chymosin protein produced bv K, lactis transformed with pGB905

g. lactis CBS 2360 (pGB905) transformants were grown 35 for 3 days at 30”C and samples were collected from the supernatant of the cultures. Protein samples were electrophoresed on a polyacrylamide gel according to Laemmli (Nature (1970) 227:680-685). Proteins were blotted onto a nitrocellulose filter according to the method of Towbin et al. (Proc. Natl. Acad. Sci. USA (1979) 76:4350-4354). Chymosin protein was detected by incubating the filter with a polyclonal antiserum against chymosin (Chr. Hansen), fol5 lowed by donkey anti-rabbit antibodies coupled to a peroxidase (Amersham) and finally with 0.6 mg/ml 4-chloronaphthol and 0.015% hydrogen peroxide in a buffer solution (50 mM Tris-HCl pH 7.5, 0.9% NaCl) containing 40% methanol. Prochymosin excreted by the AG signal sequence is correctly 10 cleaved after pH 2 treatment as demonstrated by this assay (Figure 5). Similar results were obtained with K. lactis CBS 2360 (pGB906) transformants.

Example 6 15 Construction of plasmids containing the Saccharomyces cerevisiae a-factor sequence for efficient secretion

A. Saccharomyces a-factor expression plasmids 20

1. Construction of Plasmids pDMioo-PC: The starting material was plasmid pGB163 (see EP-A-0096430, Example 16.Cl). Plasmid pGB163 was digested with BamHI and ligated to an Xbal-BamHI. a-factor 25 leader-prochymosin adaptor. The resulting mixture was then treated with PstI and a 96 bp fragment encoding the pro-afactor processing site and the N-terminal region of prochymosin was isolated. A 1900 bp fragment encoding bovine prochymosin was isolated from plasmid pJSlll following di30 gestion with PstI and Sall. Plasmid pJSlll is a pBR322 derivative containing the prochymosin gene from pGB163 under the regulatory control of the ADH-2 promoter and the glyceraldehyde 3-phosphate (GAPDH) terminator. The 1900 bp PstI to Sall fragment that was removed contains the prochymosin gene 35 and the GAPDH terminator. The yeast GAPDH 49 gene promoter and transcription terminator are essentially as described by Travis, J. Biol. Chem. (1985) 260:4384-4389.

Plasmid pDMIOO, containing a fusion of the GAPDH pro- 26 moter, the S. cerevisiae a-factor leader, and a synthetic gene for human -interferon flanked by Xbal and Sall sites and the a-factor terminator, was digested with Xbal and Sall, treated with alkaline phosphatase, then ligated to the 5 96 bp and 1900 bp fragments described above. The a-factor leader and terminator are essentially as described by Brake, Proc. Natl. Acad. Sci. USA (1984) 81:4642-4646. The resulting plasmid pDMIOO-PC was isolated and contained a fusion of the GAPDH promoter, the a-factor leader and prochymosin 10 gene. The complete sequence of the BamHI insert is shown in Figure 6.

To allow selection of yeast transformants, two plasraids, pKSlOO and pAB300, were constructed.

pKSlOO: Plasmid pKSlOO was constructed by insertion 15 into pDMIOO-PC of an 1170 bp Hindlll fragment from YEp24 containing the S. cerevisiae URA3 gene.

ΡΑΒ300: Plasmid pAB300 was constructed by insertion into pDMIOO-PC of a 3500 bp Hindlll-Sall fragment from pGB901 containing the 3' region of the K. lactis LAC4 gene 20 and the G418 resistance marker. The GAPDH/a-factor/prochymosin BamI insert in pDMIOO-PC is illustrated in Figure 6.

2. Transformation of K. lactis and S. cerevisiae Plasmid pKSlOO was digested at the Balli site in the 25 prochymosin coding region and used to transform K. lactis strain KRN201-6. This strain is a derivative of strain 2UV21 (a lac4 trpl ura3 [kil°]) in which the lac4 gene has been replaced by the LAC4 promoter-prochymosin gene fusion from pGB901. Integration of pKSlOO is thus targeted to the in30 tegrated prochymosin coding region. Plasmid pKSlOO was also used to transform S. cerevisiae strain AB110 (a ura3 leu2 his4 pep4-3 [cir⁰]), in this case targeting to the SacII site in the 3' region of the GAPDH gene.

The resulting transformants were grown to saturation 35 in liquid YEPD medium, and the culture supernatants and cell lysates assayed for chymosin activity after activation at pH

2. As shown by the results summarized in Table 5 below, the K. lactis transformants efficiently secreted prochymosin into the medium, whereas the S. cerevisiae transformants secreted only a small fraction of the prochymosin produced.

Table

Prochymosin production in K. 1actis and S, cerevisiae transformants

<td> Strain</td><td colspan="2"> Chymosin Activity (relative units/ml culture)</td>

<td> 10</td><td> Cell</td><td> Culture</td>

<td></td><td> Extract</td><td> Supernatant</td>

<td> AB110</td><td> &lt;0.25</td><td> &lt;1.0</td>

<td> AB110::pKSlOO</td><td> 15.5</td><td> 2.3</td>

<td> KRN201-6</td><td> &lt;0.25</td><td> &lt;1.0</td>

<td> 15 KRN201-6::pKSlOO</td><td> 12.0</td><td> 333.0</td>

Plasmid pAB300 was used to transform K. lactis strain 2UV21 to G418 resistance, targeting integration to the EcoRV 20 site in the 3' region of the LAC4 gene. These transformants were also found to efficiently secrete prochymosin into the culture medium as shown in Table 6 below.

Table

Prochymosin secretion from o-factor/prochymosin fusions

<td> Host Strain</td><td> Transforming Plasmid</td><td> Secreted Chymosin Activity (relative units/ml culture)</td>

<td> 2UV21 .</td><td> -</td><td> &lt;2</td>

<td> 30 KRN201-6</td><td> -</td><td> &lt;2</td>

<td> KRN201-6</td><td> pKSlOO</td><td> 385</td>

<td> 2UV21</td><td> PAB300</td><td> 294</td>

B. Construction of LAC4 nromoter/a-factor leader/ prochymosin fusions in order to produce this fusion, two intermediate plasmids were constructed. Plasmid pDMIOO-PC was partially digested with Pstl. ligated to a Sall-Psti adaptor encoding a portion of the a-factor leader and 26 bp of the region 5' to the LAC4 gene, and then digested with Hindlll. A 1500 bp fragment was isolated from this mixture and then cloned into pUC18 digested with HindiII and Sall to produce pKS102.

A synthetic E. coli lac operator was ligated into the Sall site just 5' to the a-factor leader coding sequence in pKS102 to produce the plasmid pKS103. This was done because the LAC4 promoter/a-factor leader/prochymosin fusion may be 10 toxic to E. coli.

A 490 bp Sall-Bdlll fragment from pKS103 was isolated and ligated to Sall-Bqlll-diaested pJD15R. Plasmid pJD15R is derived from pGB901 by deletion of the Sall site in the pUC19 polylinker by filling-in to produce pJD15, and then 15 recloning the 8800 bp Xbal fragment in the opposite orientation. From this reaction the plasmid pKS105 was isolated. These plasmids are illustrated in Figure 7.

Plasmid pKS105 was then used to transform K. lactis strain CBS 2360 to G418 resistance, using the Sacll site in 20 the LAC4 5' region as a targeting site for the integrative transformation. Chymosin production is expressed in units per ml of cells at 0D_r._ of 200, see Table 7 below.

<td colspan="4"> Table 7</td>

<td rowspan="2"> 25</td><td> Secretion</td><td> of Prochvmosin bv K. Lactis Cells</td><td></td>

<td></td><td> Transformed with PKS105*</td><td></td>

<td></td><td> Transformant</td><td> Supernatant</td><td> Pellet</td>

<td></td><td> 1</td><td> 111</td><td> 3.3</td>

<td> 30</td><td> 2</td><td> 147</td><td> 4.5</td>

<td></td><td> 3</td><td> 124</td><td> 3.7</td>

<td></td><td> 4</td><td> 125</td><td> 3.0</td>

* Chymosin activity in relative units/ml culture.

Example Construction of plasmids containing the Kluyveromvces lactis a-factor sequence for efficient secretion

a. Isolation and use of K. lactis a-factor signal sequence

Biological assays of culture supernatants were carried put as described (Julius et al. Cell (1983) 32:839) using as a tester strain the S. cerevisiae Mat a sst2-3 IQ strain RC687. K. lactis strain CBS 141(a) was grown in a medium consisting of 0.5% glucose, 0.17% yeast nitrogen base without ammonium sulfate (Difco), and 0.002% ammonium sulfate. After removal of cells by centrifugation, acetic acid was added to the culture supernatant to a concentration of 15 0.1 M, and the supernatant was passed over a column of BioRex 70 (Biorad). The column was washed with 0.1 M acetic acid and then the a-factor was eluted with 80% ethanol/10 mM HC1. The eluate was evaporated to dryness and then dissolved in 0.1% trifluoroacetic acid (TFA)/20% acetonitrile and 20 applied to a reverse-phase HPLC guard column. The column was washed stepwise with solutions containing 0.1% TFA and 20%, 40%, 60% and 80% acetonitrile. The 60% fraction, containing the a-factor activity, was then applied to an analytical C18 HPLC column and eluted with a gradient of 20% to 80% 25 acetonitrile in 0.1% TFA. Fractions were collected and assayed for a-factor activity. The fractions containing afactor activity were dried and subjected to amino acid sequence analysis.

B. Hybridization screening of plasmid libraries

Pools of oligonucleotides were labeled using |׳׳-[ P]ATP and T4 polynucleotide kinase. These oligonucleotide probes were used to probe Southern blots or bacterial colonies at 42’C in the following hybridization solution: 35 4xSSC, 50 mM KH₂PO₄ pH 7, 1% sarkosyl, 10% dextran sulfate, 200 Mg/ml sonicated, denatured salmon sperm DNA. Filters were washed in 2xSSC, 0.1% SDS at 42’C.

A plasmid library in the vector pJS109, containing inserts resulting from a limited Sau3AI digest of genomic

DNA from K. lactis strain SD11 (a trpl lac4), size-fractionated to purify fragments >5000 bp was screened with these probes by plating transformants of g. coli strain HB101 at a density of 500-2000 colonies per 80 mm plate of L-agar containing 100 Mg/ml ampicillin. DNA was transferred from the colonies to nitrocellulose filters and these filters hybridized as described above. Areas on the original plates corresponding to regions of hybridization signals on the fil10 ters were picked, then replated and retested by hybridization to isolate single colonies with plasmids containing hybridizing sequences. Positive colonies were further tested by Southern blot analysis of DNA purified from small cultures.

Plasmids purified from hybridization-positive colonies were digested with a variety of restriction enzymes and the resulting fragments analyzed by Southern blot analysis using the same hybridization, probes in order to identify restriction fragments of size suitable for DNA sequence analysis. Fragments thus identified were purified by agarose gel electrophoresis and cloned into appropriate MP18 and MP19 vectors. DNA sequence analysis was then performed.

C. Isolation of Kluyveromyces ot-factor

The first 10 amino acids of the K. lactis a-factor showed a definite homology to that from S. cerevisiae, with identical residues. This sequence is shown below:

Trp-Ser-Trp-Ile-Thr-Leu-Arg-Pro-Gly-Gln

This protein sequence was used to design a set of oligonucleotides deduced to be complementary to the structural gene for the corresponding structural gene as shown in

Figure 8. Oligonucleotides including all of the possible codons for a segment of the a-factor peptide were synthe35 sized as two pools of 96 and 48 different molecules.

These two pools were radioactively labeled using j⁷[^{25 * * * * 30 * * 33 * 35}P]-ATP and T₄ polynucleotide kinase, and were each used to probe a Southern blot of restriction digests of K. lactis

DNA. Pool #2 gave strong hybridization to a single fragment and much weaker hybridization to a second fragment in several different digests. Thus, pool 2 was chosen to screen plasmid libraries of K. lactis genomic DNA.

Use of these probes to screen plasmid libraries resulted in the isolation of a number of hybridizing clones. DNA sequence analysis of one of these clones, afklSb, showed it encodes an a-factor related peptide which bears a strong similarity to the precursor of the S. cerevisiae a-factor 10 peptide. The hybridizing segment was located on a Pstl-EcoRI fragment of about 1000 bp. The sequence of this fragment is shown in Figure 9. The K. lactis precursor contains only 2 sites for the addition of N-linked carbohydrate chains. In addition, the spacers of the K. lactis repeats are longer 15 than those of the S. cerevisiae repeats and show a more diverse sequence with the pattern X-Ala/Pro rather than the Glu/Ala-Pro sequences found in S. cerevisiae. A comparison of the DNA sequences showed a strong degree of homology throughout the coding region.

D. Construction of Plasmids

A series of plasmids (shown in Figure 10) were constructed in order to provide a fusion of the K. lactis afactor leader to prochymosin expressed under the transcrip25 tional control of a strong promoter.

pAB307: A 673 bp Sspl-EcoRl fragment from afkl8b (Figure 9) was modified by filling the EcoRI overhang by Klenow enzyme and addition of BglU linkers to the blunt ends. This fragment was then inserted into a Belli site 30 joining the promoter and terminator regions of the S. Cerevisiae glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH). This cassette was cloned as a BamHI fragment in pUC18, resulting in pAB307.

pAB309: Fusion of sequences encoding the a-leader and 35 bovine prochymosin was then performed. First pAB307 was digested with Ncol and the cohesive ends made blunt by treatment with mung bean nuclease. The resulting product was then digested with Sall. To this was ligated a 2000 bp EcoRV-Sall fragment containing sequences encoding prochymosin and the

S. cerevisiae transcriptional termination region. This fragment was derived from plasmid pJSlll in which a Xbal-BamHI adaptor had been added to the 5' end of a fragment con5 taining prochymosin cDNA fused to the S. cerevisiae GAPDH transcriptional termination region. This ligation mixture was used to transform E. coli strain HB101 and a transformant carrying the plasmid pAB309 was isolated. The sequences around the junction of this fusion are shown in 10 Figure 11 and the sequence of the entire BamHI-Sall insert of pAB309 is shown in Figure 12.

ρΆΒ312: In order to obtain transformation of K. lactis strains, a 3560 bp Hindlll fragment derived from pGB901 was inserted into pAB309 producing plasmid pAB312. The 15 Hindlll fragment contains the 3' region of the K. lactis LAC4 gene and a fusion of the S. cerevisiae ADH1 promoter to the bacterial G418-resistance structural gene.

pAB313 and pAB314: A 1900 bp S'acl-Hindlll was isolated from pAB309 and cloned into MP19 (Yanisch-Perron et 20 at., Gene (1985) 33:103). Single-stranded phage DNA was prepared and used as a template for in vitro mutagenesis with one of the two oligonucleotide primers shown in Figure

13. The M13 phage MP19/akll.5 and MP19/akl2.2 were prepared using Primer #1 and Primer #2, respectively.

Double-stranded RF DNA was prepared from these phages, and 1100 bp Sacl-Stul fragments isolated from each. These fragments were ligated to a 7100 bp Sacl-Stul fragment from pAB312. The resulting plasmids pAB313 and pAB314 were isolated with the sequence alterations illustrated in Figure 30 13.

E. Transformation of Kluweromvces

Plasmid pAB312 was digested with EcoRV (to target integration to the LAC4 region of the K. lactis genome) and 35 was then used to transform K. lactis strain 2UV21 (a ura3 trpl lac4 [kil°]) to G418 resistance. The plasmids pAB313 and pAB314 were used to transform strain 2UV21 to G418 resistance. Cultures of transformants 2UV21::pAB312,

2UV2i::pAB313 and 2UV21::pAB314 were grown and culture supernatants assayed for chymosin activity as above.

A number of these transformants, as well as an untransformed control strain, were grown for 36 hours in 1 ml 5 of medium composed of 1% yeast extract, 2% peptone, 2% glucose, 0.17% yeast nitrogen base, 50 Mg/ml tryptophan and 50 Mg/ml uracil. Culture supernatants were then assayed for chymosin activity after acid activation. All of the transformants were found to secrete activatable chymosin. The 10 results are shown in the following Table 8.

Table

Secretion of prochymosin in Kluyveromyces

<td> 15</td><td> Strain</td><td> Host</td><td> Plasmid</td><td> Chymosin Activity (relative units/ml culture)</td>

<td></td><td> 2UV21</td><td> 2UV21</td><td> -</td><td> &lt;2</td>

<td></td><td> KRN303-1</td><td> 2UV21</td><td> pAB312</td><td> 256</td>

<td></td><td> KRN304-4</td><td> 2UV21</td><td> ΡΑ.Β313</td><td> 175</td>

<td> 20</td><td> KRN305-2</td><td> 2UV21</td><td> pAB314</td><td> 206</td>

Each of the transformants was found to secrete a single prochymosin-related species as judged by SDS poly25 acrylamide gel electrophoresis of trichloroacetic acid-precipitated culture supernatants. The prochymosin-related protein secreted by pAB312 transformants appeared to be of slightly higher molecular weight than those secreted by pAB313 and pAB314 transformants as determined by electropho30 retie mobility.

The major species secreted by KRN303-1 and KRN304-4 were purified by preparative SDS polyacrylamide gel electrophoresis and subjected to gas phase amino acid sequence analysis. The N-terminal sequences of these species are 35 given below.

KRN-303-1

5 10 15

Glu-Ala-Asp-Ala-Ser-His-His-Met-Ala-Glu-Ile-Thr-Arg-Ile-Pro

KRN304-4

5

Ala-Glu-Ile-Thr-Arg-Ile

These results indicate that the prochymosin-related species secreted by KRN303-1 has undergone no processing of the amino-terminal spacer sequence, while the species secreted from KRN304-4 has the authentic mature prochymosin amino terminus.

Example

Secretion of t-PA bv Kluyveromyces lactis using an amyloglucosidase signal seguence

A. Cloning of tissue-type plasminogen activator cDNA

A cDNA coding for tissue-type plasminogen activator (t-PA) was obtained in a way similar to that described by Pennica et al. (Nature (1983) 301:214). DNA sequence analysis and restriction mapping confirmed the authenicity of the t-PA cDNA. For expression studies the 2.0 kb Bglll fragment (see Pennica et al.). comprising almost the complete coding region for the mature protein and the 3' noncoding region, was used.

B. Introduction of the G418 resistance marker in pUC19

A DNA fragment comprising the Tn5 gene (Reiss et al., EMBO J. (1984) 3:3317), conferring resistance to G418 under the direction of the alcohol dehydrogenase I (ADHI) promoter from S. cerevisiae, similar to that described by Bennetzen and Hall, J. Biol. Chem. (1982) 257:3018, was inserted into the Smal site of pUC19 (Yanisch-Perron et al., Gene (1985) 33:103). The obtained plasmid, pUCG418, is shown in Figure

14. E. coli containing pUCG418 was deposited at Centraal Bureau voor Schimmelcultures on December 4, 1987 under CBS 872.87.

C. Construction of pGBtPAl

In a few cloning steps pGBtPAl was constructed (see also Figure 15 and Table 9) containing the following elements:

(1) pUCG418 (see above) cut with Xbal and Hindlll;

(2) the Xbal-Sall fragment from pGB901, containing the lactase promoter;

(3) synthetic DNA coding for the signal sequence of amyloglucosidase from Aspergillus awamori (Innis et al., Science (1985) 228:21). The sequence in front of the startcodon was chosen to remove the Sall site at the end of the lactase promoter fragment and further comprises part of the 5’ noncoding region of the lactase gene;

(4) the 2.0 kb Bglll fragment from the t-PA cDNA (see above);

(5) synthetic DNA, comprising part of the 3' noncoding region of_the lactase gene.

Table

Schematic representation of plasmid pGBtPAl

Xbal v .....pUCG418.....TCTAGA...lactase promoter...

......GTCGATCATCGAGAACTGAAAGATATGTCTTGCCTTATGTCTTTCAGA

TCCCTACTAGCTCTATCCGGTCTAGTTTGTACTGGTCTAGCTAACGTTATCTCCAAGAG

Sall Bglll Bglll

V V V

AGTCGACAGATCT.....t-PA cDNA...AGATCTGATATGAATTTATACT

TAGATAAGTATGTACTTAGATAAGTATGTACTTACAGGTATATTTCTATGAGATACTGA

Hindlll v TGTATACATGCATGATAATATTTAAAGCTT

Protein synthesis starts at the underlined ATG codon.

D. Transformation of Kluyveromyces lactis and analysis of the transformants.

Transformation of K. lactis strain CBS 2360 with pGBtPAl was performed as described in Example 2. The transformants and the control strain CBS 2360 were grown in YEPDmedium for about 64 hrs. at 30°C. Cells were separated from the culture medium by centrifugation. The cells were resuspended in a physiological salt solution at an OD₆₁₀ of 300 and disrupted by shaking with glass beads for 3 minutes on a Vortex shaker at maximal speed. Cell debris was removed by centrifugation.

A clotlysis assay according to Wallen et al. (Biochim. Biophys. Acta (1982) !19:318) was performed in microtiter plates. A solution was made, containing 15 mM phosphate buffer (pH 7.3), 0.2 CU/ml plasminogen, 1.5 mg/ml fibrinogen and 0.04% gelatin._ Into each well of a microtiter plate 10 μΐ thrombine (13.9 NIH units/ml), 25 μΐ sample and 65 μΐ of the plasminogen/fibrinogen solution were mixed. The reaction was followed by measuring the OD₄₅q every 30 min. t-PA from melanoma cells (Kabi Vitrum) was used to provide a calibration curve within every microliter plate.

Table 10 shows the result of a typical analysis of 10 transformants. It is demonstrated that t-PA was found in the culture medium of K. lactis transformed with pGBtPAl.

/

Table

Clotlysis assay of the cultures from CBS 2360 and from CBS 2360 transformed with pGBtPAl

<td> transformant</td><td> t-PA activity in supernatant</td>

<td> 1</td><td> 40 μ9/1</td>

<td> 2</td><td> 6 μg/l</td>

<td> 3</td><td> &lt;3 μ9/1</td>

<td> 4</td><td> &lt;3 μα/1</td>

<td> 5</td><td> 25 _Mg/1</td>

<td> 6</td><td> 3 Mg/l</td>

<td> ר</td><td> 3 μ9/1</td>

<td> 8</td><td> &lt;3 μ9/1</td>

<td> 9</td><td> 3 μ?/1</td>

<td> 10</td><td> &lt;3 μg/l</td>

<td> CBS 2360 1°)</td><td> &lt;3 μg/l</td>

<td> CBS 2360 2’)</td><td> &lt;3 μg/l</td>

<td> CBS 2360 3°)</td><td> &lt;3 μg/l</td>

<td> CBS 2360 4°)</td><td> &lt;3 μ9/1</td>

<td> CBS 2360 5°)</td><td> &lt;3 μ9/1</td>

In some of the cell extracts a slight t-PA activity (&lt; 3 gg/l) was found.

Analysis was also performed on SDS-polyacrylamide gels overlayered with a plasminogen/fibrin-agarose gel according to Granelli-Piperno and Reich (J. Exp. Med. (1978) 148:223). 200 μΐ of the supernatant of a culture of CBS 2360 or CBS 2360 transformed with pGBtPAl was precipitated with ethanol and resuspended in 20 μΐ sample buffer (62.5 mM Tris-HCl pH 6.8, 2% sodium dodecylsulphate, 10% glycerol, Bromophenol Blue). The samples were layered on the gels without prior boiling. The results (shown in Figure 16) demonstrate the secretion of human t-PA by .-K. lactis. Furthermore, it is clear that most of the secreted material is glycosylated.

The secretion of t-PA was also confirmed by using an ELISA with a monoclonal antibody against human t-PA (ESP5 purchased from Biopool) and by a chromogenic activity assay (a commercial test from Kabi Vitrum).

Example

Secretion of t-PA by Kluweromvces lactis using the signal sequence from human serum albumin

A. Construction of pGBtPA2 In a few cloning steps pGBtPA2 was constructed (see

Figure 17 and Table 11), containing the following elements :

(1) pUCG418, cut with Xbal and HindHI;

(2) the Xbal-Sall fragment from pGB901, containing the lactase promoter;

(3) synthetic DNA coding for the prepro-region of human serum albumin;

(4) the 2.0 kb Bglll fragment from the t-PA cDNA (see Example 8);

(5) synthetic DNA, comprising part of the 3' noncoding region of the lactase gene.

Table

Schematic representation of plasmid pGBtPA2

Xbal v

.....pUCG418.....TCTAGA....lactase promoter.

Sall v

.......GTCGACAAAAAATGAAGTGGGTTACCTTCATCTCCTTGTTGTTCTT

Bull I v GTTCTCCTCCGCTTACTCCAGAGGTGTTTTCAGAAGAGGTGCTAGATCT.....

EcoRV Bglll Xhol v v v t-PA CDNA...........AGATCTGATATCTCGAGAATTTATACTTA

GATAAGTATGTACTTACAGGTATATTTCTATGAGATACTGATGTATACATGCATGATAA

Hindlll V TATTTAAAGCTT.......

Protein synthesis starts at the underlined codon.

B. Transformation of K. lactis and analysis of the transformants Transformation of CBS 2360 with pGBtPA2 was performed as described in Example 8. The transformants and the control 5 strain were grown and treated as described in the previous Example. The results of the clotlysis assay are summarized in Table 12.

Table

Clotlysis assay of the cultures of CBS 2360 and

CBS 2360 transformed with pGBtPA2

<td> transformant__________</td><td> t-PA activitv in the supernatant</td>

<td> 1</td><td> 25 Mg/1</td>

<td> 15 2</td><td> 25 pg/l</td>

<td> 3</td><td> &lt;3 Mg/1</td>

<td> 4</td><td> &lt;3 Mg/1</td>

<td> 5</td><td> 6 Mg/1</td>

<td> . . __₆ _ --- -----</td><td> — _ —12 Mg/-1</td>

<td> 20 7</td><td> &lt;3 Mg/1</td>

<td> 8</td><td> &lt;3 Mg/1</td>

<td> 9</td><td> &lt;3 Mg/1</td>

<td> 10</td><td> &lt;3 Mg/1</td>

<td> CBS 2360 1°)</td><td> &lt;3 Mg/1</td>

<td> 25 CBS 2360 2°)</td><td> &lt;3 Mg/1</td>

<td> CBS 2360 3’)</td><td> &lt;3 Mg/1</td>

<td> CBS 2360 4״)</td><td> &lt;3 Mg/1</td>

<td> CBS 2360 5°)</td><td> &lt;3 Mg/1</td>

In some of the cell extracts a slight t-PA activity (&lt; 3 gg/1) was found.

Example 10

Secretion of human serum albumin by Kluyveromyces lactis

A. Synthesis and cloning of the HSA cDNA cDNA encoding human serum albumin was prepared accor40 ding to the method of Okayama and Berg (Mol. Cell. Biol.

(1982) 2.: 161) using mRNA prepared from human liver. The cDNA was cloned in a vector derived from pBR322 and transformed to E. coli. Screening of the transformants was performed using a oligodesoxyribonucleotide based on the sequence of the HSA cDNA clone of Lawn et al., Nucleic Acids Res. (1981) 9:6103). The selected cDNA clone was partially sequenced and compared to the sequence of Lawn et al. This revealed that the first 5 nucleotides of the preproHSA coding region were absent, but the BstEII site at nucleotides 9-15 of the coding region was still found. This BstEII site and the Hindlll site in the 3’ noncoding region (see Lawn et al., cited above) were used for subcloning in expression vectors.

B. Construction of pGBHSAl

In a few cloning-steps pGBHSAl was constructed, containing the following elements:

(1) pUCG418 (see Example 8) cut with Xbal and Hindlllז (2) the Xbal-Sall fragment from pGB901 (see Example

1)?________ _______ _ _ (3) synthetic DNA (Sall-Hindlll fragment), comprising part of the 3' noncoding region of the lactase gene. The sequence of this fragment is given in Table 13.

Table

Sequence of the Sall - Hindlll fragment of pGBHSAl

EcoRV

Sall Notl Bqlll Xhol

V V V v v

TCGACGCGGCCGCAGATCTGATATCTCGAGAATTTATACTTAGATAAGTATGTACTTACA GCGCCGGCGTCTAGACTATAGAGCTCTTAAATATGAATCTATTCATACATGAATGT

Hindlll v GGTATATTTCTATGAGATACTGATGTATACATGCATGATAATATTTAA CCATATAAAGATACTCTATGACTACATATGTACGTACTATTATAAATTTCGA

C. Construction of PGBHSA2 pGBHSAl was cut with Sal I and EcoR V and synthetic DNA was inserted:

Sall BstEII Stul v v v

TCGACAAAAATGAAGTGGGTAACCATCGATAGGCCTACTGGGCTCGAGATC _{GTTTTTACTTCACCC}attggtagctatccggatgacccgagctctag

The underlined ATG-codon indicate the initiation codon in the ultimate expression construct (pGBHSA3, see below).

The resulting plasmid was named pGBHSA2.

D. Construction of PGBHSA3

The HSA cDNA-clone was cut with Hindlll and the sticky end was filled in, using Klenow DNA polymerase I. Subsequently the DNA was cut with BstEII and the BstEIIHindlll (filled in) fragment, containing almost the complete HSA coding region was purified. pGBHSA2 was digested with Xhol. the sticky ends were filled in using Klenow DNA polymerase I and digestion with BstEII was performed. In the resulting vector the HSA encoding fragment (BstEIIHindlll (filled in)) was inserted. The obtained plasmid, pGBHSA3, is shown in Figure 18.

E. Transformation of Kluyveromyces lactis and analysis of the transformants Transformation of K. lactis strain 2360 with pGBHSA3 was performed as described in Example 2. Transformants and the control strain CBS 2360 were grown in YEPD medium for about 64 hrs at 30’C. The cells were separated from the culture medium by centrifugation. Samples of 30 μΐ were taken from the supernatants and analysed by electrophoresis on a 10% polyacrylamide gel according to Laemmli (Nature 227, 680 (1970)). The results shown in Figure 19 demonstrate that HSA is secreted into the culture medium by K. lactis cells transformed with pGBHSA3. There is also an indication that the secretion of other proteins is reduced in the HSA producing cells.

The above results demonstrate that one can obtain efficient, convenient expression of exogenous genes in Kluyveromvces strains. Furthermore, the Kluyveromyces strains appear to be particularly useful for providing highly efficient secretion and processing of a wide variety of proteins, as illustrated by the results with prochymosin. Constructs and vectors are provided which allow for the introduction of an exogenous gene under the regulatory control of efficient promoters in Kluyveromvces and, as desir״ ed, joining to signal sequences which provide for translocation of the exogenous gene, particularly secretion. Thus, a fermentation system is provided for commercial production of a wide variety of exogenous proteins in an active or activatable form.

The following organisms have been deposited with the American Type Culture Collection on June 30, 1987: 2UV21, ATCC Accession No. 20855KRN201-6, ATCC Accession No. 20854; HB.101 pAB307, ATCC Accession No. 67484; HB101 pAB312, ATCC Accession No. 67455.

Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims.

Although the foregoing invention has been described in some detail by way of illustration and example for purpose of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims.