1h-imidazo(4,5-c)quinolines and pharmaceutical compositions containing certain such compounds

This invention relates to certain 1H-imidazo[4,5-c] quinoline compounds, some of which are useful as bronchodilators and the others as intermediates for the preparation of the former compounds and also for the preparation of the antiviral compounds which are disclosed and claimed in Israel Patent Specification No. 73534, from which the present application was divided out.

Background of the Invention

The earliest report of an imidazo[4,5-c]quinoline ring system was by Backeberg et al, J. Chem. See., 972-977 (1938). However, his report of 4-methyl-lH-imidazo[4,5-c]quinoline and 2,4-dimethyl-lH-imidazo[4,5-c]quinoline (named as 2-methylquin (3:4:5 ' :4' ) iminazole and 2: 2 ’־rdimethylquin(3:4:5’:4¹)iminazole) is known to be erroneous in view of later work of Koenigs and Freund, Chemische Berichte 80, 143 (1947).

A further report by Backeberg, J. Chem. Soc., 1083-1089 (1938) of 2,4-dimethyl-3-phenyl-3H-imidazo[4,5-c]quinoline (named 1¹-phenyl-2:2’-dimethylquin(3:4:5¹:4')iminazole) is also known to be erroneous in view of the above work of Koenigs and Freund.

The first reliable report of a lH-imidazo[4,5-c]quinoline is by Bachman et al., J. Org. Chem. 15, 1278-1284 (1950) who synthesized 1-(6-methoxy-8-quinolinyl)-2-methyllH-imidazo[4,5-c]quinoline as a possible antimalarial agent.

Surrey et al, J. Am. Chem. Soc. 73, 2413 (1951) synthesized certain 3-nitro- and 3-amino-4-dialkylaminoalkylaminoquinolines as possible antimalarial and antibacterial agents.

Jain et al., J. Med. Chem. 11, pp. 87-92, (1968), synthesized the compound [2-(4-piperidyl)ethyl]-lH-imidazo[4,5-c]quinoline as a possible anticonvulsant and cardiovascular agent.

Baranov et al., Chem. Abs . 85, 94362 (1976), reported several 2-oxoimidazo[4,5-c]quinolines.

Abbasi et al., Monatsh. Chem. Ill (4), pp 963-969 (1980), reported certain 2H-3-hydroxyimidazo[4,5-c]quinolines.

Berenyi et al, J. Heterocyclic Chem. 18, 1537-1540 (1981), reported certain 2-oxoimidazo[4,5-c]quinolines.

U.S. Patent No. 3,700,674 (Diehl et al.) describes certain 4-alkylamino-3-nitroquinolines as herbicidal compounds.

Detailed Description of the Invention

This invention relates to compounds of Formula A

<img file="IL84537A_D0001.tif" />

(A) wherein

R* is selected from the group consisting of hydrogen, alkyl, cycloalkyl or cycloalkylalkyl having each up to about ten carbon atoms, hydroxyalkyl of one to about six carbon atoms, acyloxyalkyl wherein the acyloxy moiety is alkanoyloxy of two to about four carbon atoms or benzoyloxy, and the alkyl moiety contains one to about six carbon atoms, benzyl, (phenyl)ethyl and phenyl, said benzyl, (phenyl)ethyl or phenyl substituent being optionally substituted on the benzene ring by one or two moieties independently selected from the group consisting of alkyl of one to about four carbon atoms, alkoxycarbonylalkyl wherein the alkyoxy moiety contains one to about four carbon atoms and the alkyl moiety contains one to about three carbon atoms, alkoxy of one to about four carbon atoms and halogen, with the proviso that if said benzene ring is substituted by two of said moieties, then said moieties together contain no more than 6 carbon atoms;

R* is selected from the group consisting of hydrogen, trifluoromethyl, hydroxyalkyl. of one to about six carbon atoms, aminoalkyl of one to about four carbon atoms, alkanamidoalkyl wherein each alkyl radical is one to about four carbon atoms, benzylthio, mercapto, alkylthio of one to about four carbon atoms, and alkyl of one to about eight carbon atoms;

R* is selected from the group consisting of hydrogen, chloro, alkoxy of one to about four carbon atoms, hydroxy, alkylamino of one to about four carbon atoms, dialkylamino wherein each alkyl radical contains one to about four carbon atoms, alkyl of one to about four carbon atoms, phenyIthio, alky!thio of one to about four carbon atoms, and morpholino, with the proviso that when R* is mercapto, alkylthio or benzylthio, R£ is hydrogen or alkyl;

R* is independently selected from the group consisting of alkoxy of one to four carbon atoms, alkyl of one to four carbon atoms, and halogen, ard n is an integer from 0 to 2, with the proviso that if n is 2, then said R* substituents together contain no more than 6 carbon atoms;

or the N-oxide of said compound involving the nitrogen atom in the 5-position; with the further following provisos:

(i) when R* is other than chloro or said compound is other than said N-oxide, then R* can not be acyloxyalkyl;

(ii) when R* is chloro or said compound is said N-oxide, then R* is other than benzylthio, mercapto, or alkylthio;

(iii) when said compound is said N-oxide, then R* is hydrogen;

or a pharmaceutically acceptable acid addition salt thereof except when R* is chloro or said compound is said N-oxide.

Among the compounds of the present invention, some possess bronchodilator activity and.are represented by Formula I

I

<img file="IL84537A_D0002.tif" />

wherein R]_ is selected from the group consisting of hydrogen, alkyl of one to about ten carbon atoms, cycloalkyl or cycloalkylalkyl having each up to about ten carbon atoms, hydroxyalkyl of one to about six carbon atoms, benzyl, (phenyl)ethyl and phenyl, the benzyl, (phenyl)ethyl or phenyl substituent being optionally substituted on the benzene ring by one or two moieties independently selected from the group consisting of alkyl of one to about four carbon atoms, alkoxycarbonylalkyl wherein the alkoxy moiety contains one to about four carbon atoms and the alkyl moiety contains one to about three carbon atoms, alkoxy of one to about four carbon atoms and halogen, with the proviso that when the benzene ring is substituted by two of said moieties, then the moieties together contain no more than 6 carbon atoms: R₂ is selected from the group consisting of hydrogen, trifluoromethyl, hydroxyalkyl of one to about six carbon atoms, aminoalkyl of one to about four carbon atoms, alkanamidoalkyl wherein each alkyl radical is one to about four carbon atoms, benzylthio, mercapto, alkylthio of one to about four carbon atoms, and alkyl of one to about eight carbon atoms: R4 is selected from the group consisting of hydrogen, alkyl of one to about four carbon atoms, alkoxy of one to about four carbon atoms, hydroxy, alkylamino of one to about four carbon atoms, dialkylamino wherein each alkyl radical contains one to about four carbon atoms, phenylthio, alkylthio of one to about four carbon atoms, and morpholino, with the proviso that when R2 is mercapto, alkylthio or benzylthio, R4 is hydrogen or alkyl: and each R is independently selected from the group consisting of alkoxy of one to about four carbon atoms, alkyl of one to about four carbon atoms, and halogen, and n is an integer from 0 to 2, with the proviso that when n is 2, then the R substituents together contain no more than 6 carbon atoms; and pharmaceutically acceptable acid addition salts thereof. Some of the compounds of Formula I are also useful antiviral agents.

Among the compounds of Formula A are two groups of novel intermediates for.the preparation of the bronchodilator compounds of Formula I or for the preparation of antiviral compounds in Israel Patent Specification No. 75534. One such group of intermediates has the Formula XXII

<img file="IL84537A_D0003.tif" />

XXII wherein Rg is selected from the group consisting of alkyl of one to about ten carbon atoms, cycloalkyl or cycloalkylalkyl having each up to about ten carbon atoms, hydroxyalkyl of one to about six carbon atoms, acyloxyalkyl wherein the acyloxy moiety is alkanoyloxy of two to about four carbon atoms or benzoyloxy, and the alkyl moiety contains one to about six carbon atoms, benzyl, (phenyl)ethyl and phenyl, the benzyl, ( phenyl)ethyl or phenyl substituent being optionally substituted on the benzene ring by one or two moieties independently selected from the group consisting of alkyl of one to about four carbon atoms, alkoxycarbonylalkyl wherein the alkoxy moiety contains one to about four carbon atoms and the alkyl moiety contains one to about three carbon atoms, alkoxy of one to about four carbon atoms and halogen, with the proviso that if the benzene ring is substituted by two of said moieties, then said moieties together contain no more than 6 carbon atoms; Rg is selected from the group consisting of hydrogen, trifluoromethyl, hydroxyalkyl of one to about six carbon atoms, aminoalkyl of one to about four carbon atoms, alkyl of one to about eight carbon atoms and alkanamidoalkyl wherein each alkyl radical is one to about four carbon atoms; and each R₅ is independently selected from the group consisting of halogen, alkoxy of one to about four carbon atoms, and alkyl of one to about four carbon atoms, and n is an integer from 0 to 2, with the proviso that if n is 2, then the R₅ substituents together contain no more than 6 carbon atoms.

Another such group of intermediates has the Formula

XXIII

XXIII wherein Rg is selected from the group consisting of hydrogen, alkyl of one to about ten carbon atoms, cycloalkyl or cycloalkylalkyl having each up to about ten carbon atoms,, hydroxyalkyl of one to about six carbon atoms, acyloxyalkyl wherein the acyloxy moiety is alkanoyloxy of two to about four carbon atoms or benzoyloxy, and the alkyl moiety contains one to about six carbon atoms, benzyl, (phenyl)ethyl and phenyl, the benzyl, (phenyl)ethyl, or phenyl substituent being optionally substituted on the benzene ring by one or two moieties independently selected from the group consisting of alkyl of one to about four carbon atoms, alkoxycarbonylalkyl wherein the alkoxy moiety contains one to about four carbon atoms and the alkyl moiety contains one to about three carbon atoms, alkoxy of one to about four carbon atoms, and halogen, with the proviso that if the benzene ring is substituted by two of said moieties, then said moieties together contain no more than 6 carbon atoms; Rg is selected from the group consisting of hydrogen, trifluoromethyl, hydroxyalkyl of one to about six carbon atoms, aminoalkyl of one to about four carbon atoms, alkanamidoalkyl wherein each alkyl radical is one to about four carbon atoms, and alkyl of one to about eight carbon atoms; and each R^ is independently selected from the group consisting of halogen, alkoxy of one to about four carbon atoms, and alkyl of one to about four carbon atoms, and n is an integer from 0 to 2, with the proviso that if n is 2, then the R^ substituents together contain no more than 6 carbon atoms.

The compounds of Formulae XXII and XXIII are useful intermediates in the preparation of the compounds of Formula I and of some of the compounds of Israel Patent Application No. 75534.

Some of the compounds of Formula I are aryl or alkyl amines and those that are may be used in the form of acid addition salts such as hydrochlorides, dihydrogen sulfates, trihydrogen phosphates, hydrogen nitrates, methane sulfonates and salts of other pharmaceutically acceptable acids. Pharmaceutically acceptable acid-addition salts of compounds of Formula I are generally prepared by reaction of the respective compound with an equimolar amount of a relatively strong acid, preferably an inorganic acid such as hydrochloride, sulfuric or phosphoric acid or an organic acid such as methanesulfonic acid in a polar solvent. Isolation of the salt is facilitated by the addition of a solvent in which the salt is insoluble, an example of such a solvent being diethyl ether.

Generally, alkyl moieties which may be contained in the compounds of the invention may be straight or branchedchain or cyclic.

R* (Formula A), R^ (Formula I) and Rg (Formulae XXII and XXIII) substituents which are alkyl preferably contain one to about eight carbon atoms, and more preferably contain about four to about six carbon atoms.

R* (Formula A) , R״ (Formula I) and R_g (Formulae XXII and XXIII) substituents which are alkyl preferably contain one to about four carbon atoms.

Hydroxyalkyl substituents which may be contained in the compounds of the invention preferably contain one to about four carbon atoms.

The remaining substituents which may be contained in the compounds of the invention and contain an alkyl radical such as the substituents alkoxy, aminoalkyl, alkylthio, alkyl amino, dialkylamino and alkyl (other than R* , R_1׳ Rij, R₂ and/or

R as alkyl) preferably contain one or two carbon atoms in each 8 alkyl radical.

The preferred R^ seven carbon atoms.

cyclic alkyl moieties contain six or

The halogen substituents which may be contained in the compounds of the instant invention are selected from fluorine, chlorine and bromine. Preferred halogen substituents are fluorine and chlorine.

It is preferred that n of Formulae A, I, XXII and XXIII be zero or one. It is most preferred that n of Formulae I, XXII and XXIII be zero.

If R* of Formula A and R. of Formula I or R of 1 16

Formulae XXII or XXIII is substituted benzyl, (phenyl)ethyl or phenyl, it is preferred that the benzene ring be mono-substituted. It is most preferred that the benzyl, (phenyl)ethyl or phenyl substituent be unsubstituted. As used in the instant specification and claims, (phenyl)ethyl denotes 1-(phenyl)ethyl or 2-(phenyl)ethyl.

It is presently preferred that R^ of Formulae A and I be alkyl, benzyl, (phenyl)ethyl, cyclohexylmethyl or hydroxyalkyl. When R^ of Formula I is cyclic alkyl, it is preferably cyclohexylmethyl.

When R* of Formula A and R^ of Formula I is hydroxyalkyl, they may contain from one to three hydroxy substituents. Preferred hydroxyalkyl groups contain one or two hydroxy substituents.

Presently preferred bronchodilator compounds of

Formula I are:

1.8- dimethyl-2-hydroxymethyl-lH-imidazo[4,5-c]quinoline,

1.8- dimethy1-2-trifluoromethy1-1H-imidazo[4,5-c]quinoline, l-methyl-4-methoxy-lH-imidazo[4,5-c]quinoline , l-isobutyl-8-methyl-lH-imidazo[4,5-c]quinoline!

1-ethy1-2-methy1-lH-imidazo[4,5-c]quinoline, l-ethyl-lH-imidazo[4,5-c]quinoline , l-phenyl-lH-imidazo[4,5-c]quinoline ,

1-(4-fluoropheny1)-lH-imidazo[4,5-c]quinoline, and

1-isobutyl-lH-imidazo[4,5-c]quinolin-4-01.

Compounds of the invention of Formula I wherein R!, r₂, R and n are aa defined above, and R4 is hydrogen are prepared as described in the first three steps of the Reaction Scheme A below. Compounds of the invention of Formula I wherein Rj_, R2׳ &amp; and n are ^as defined above, and R4 is alkoxy, alkylamino, dialkylamino, phenylthio, alkylthio, morpholino or hydroxy are prepared by further reaction of intermediates of Formula VIII or IX as shown in the latter steps of the Reaction Scheme below.

-סו84537/3

Reaction Scheme A

<img file="IL84537A_D0004.tif" />

-ווMany quinolines of Formula IV are known compounds (see, e.g., U.S. Patent 3,700,674 and references described therein). Those which are not may be prepared by known methods, for example, from 4-hydroxy-3-nitroquinolines as illustrated in step (1) of the Reaction Scheme. Step (1) may be conducted by reacting the 4-hydroxy-3-nitroquinoline of Formula III with phosphorus oxychloride. The reaction is preferably conducted in Ν,Ν-dimethylformamide and is accompanied by heating. A large molar excess of phosphorus oxychloride is preferably avoided. Employment of about a

1-2 molar ratio of phosphorus oxychloride to the

4-hydroxy-3-nitroquinoline has been found to be particularly suitable. Some compounds of Formula V are known such as those wherein Rj_ is optionally substituted (phenyl)ethyl, 6-methoxy-8-quinolinyl, dialkylaminoalkyl, and phenyl. However, compounds of Formula V wherein R₃ is cyclohexylmethyl or hydroxyalkyl are novel.

In step (2), an optionally substituted 3-nitro-4chloroquinoline of Formula IV is reacted by heating with an amine of the formula R]_NH2 in a suitable solvent such as water or tetrahydrofuran to provide a quinoline of Formula V wherein R₄ is hydrogen or alkyl.

Steps (1) and (2) may be combined such that the

3-nitro-4-chloroquinoline need not be isolated prior to reaction with the amine. Such a reaction is exemplified in Example 168 and Example 214 (Step A) below.

Compounds of Formula V are catalytically reduced in step (3) using a platinum catalyst such as platinum on charcoal to provide compounds of Formula VI. The reduction is conveniently carried out on a Parr apparatus in a non-reactive solvent such as toluene or a lower alkanol. Compounds of Formula VI wherein Rj is cyclohexylmethyl or hydroxyalkyl are novel.

In step (4) the intermediate compounds of Formula VI are reacted with a dialkoxyalkyl alkanoate such as diethoxymethyl acetate, or a carboxylic acid which can introduce the desired R₂ group, or a trialkyl ortho ester

־12-

סו

5ו of the formula R2C(Oalkyl)3t wherein alkyl” i3 an alkyl group containing 1 to about 4 carbon atoms, or the combination of such a trialkyl ortho ester and such a carboxylic acid to provide a novel compound of Formula VII, which is a subgroup of the compounds of Formula I, The reaction of step (4) is carried out , by heating, e.g., at about 130״C, in the presence of an acid, preferably an alkanoic acid having one more carbon atom than R₂. Suitable acids also include haloalkanoic acids, aminoalkanoic acids, hydroxyalkanoic acids and the like. Carbon disulfide may also be used in the presence of strong base to provide compounds wherein ^2 ^-SH. The compounds of Formula VII are active as bronchodilators. In addition, compounds of Formula VII are particularly useful as intermediates to provide other compounds of Formula I as described below.

Step (5) provides a novel intermediate of Formula VIII through oxidation of the compound of Formula VII with a typical oxidizing agent used to form N-oxides. Suitable oxidizing agents include peracids and hydrogen peroxide. The oxidation reaction is preferably conducted in glacial acetic acid. Heating is generally employed to accelerate the rate of reaction.

Steps (4) and (5) may be combined such that the compound of Formula VII need not be isolated prior to reaction with the oxidizing agent. Such a reaction is exemplified in Example 214 (Step C) below.

In step (6) the N-oxide of Formula VIII is converted to the 4-chloro intermediate of Formula IX by heating in the presence of a suitable chlorinating agent such as phosphorus oxychloride or thionyl chloride. Phosphorus oxychloride is the preferred chlorinating agent and it is preferred that it be used in combination with Ν,Ν-dimethylformamide as the solvent.

In step (7) the 4-chloro group of the compound of

Formula IX is replaced with alkoxy, alkylamino, dialkylamino, phenylthio, alkylthio, or morpholino by

־13־ reacting the compound of Formula IX with an alkoxide, _analkylamine, a dialkylamine, phenylthiol, an alkanethiol, or morpholine, respectively to provide a compound of the invention of Formula X. The reaction is carried out by heating the reactants, generally at reflux, in an inert solvent. In order to prepare compounds of Formula X wherein R4 is -OH, an intermediate of Formula VIII is heated with acetic anhydride as shown in step (8).

Compounds of Formula I of the invention wherein R₂ is alkanamidoalkyl are prepared by acylation of compounds wherein R2 is aminoalkyl. Compounds of Formula I of the invention wherein R2 is alkythio or benzylthio are prepared by alkylation or benzylation of the corresponding mercapto compound.

For compounds wherein Rj of Formula I is hydroxyalkyl, the synthesis illustrated in the Reaction Scheme A above is preferably modified. Specifically, it is generally necessary to first block or protect the hydroxy group with an acyloxy group such as alkanoyloxy or benzoyloxy for step(s) (5) and/or (6) and/or (7), and to then remove the blocking group. Such blocking reactions are exemplified in Examples 119-122, 124-127 and 134 below.

The bronchodilator activity of the compounds of Formula I was assessed by the measurement of effects on isolated tracheal spirals. This is a well-known and conventional test method. The in vitro bronchodilator activity was determined as follows: Female guinea pigs were sacrificed, and each trachea removed and cut into a spiral strip. This strip was mounted in a constant temperature (37°C) muscle bath having a volume of approximately 15 ml. The bathing medium was Krebs-Henseleit solution. Movement of the tracheal strip was measured by means of an isometric transducer connected to an electric recorder. The bath was aerated with a mixture of 95% carbon dioxide and 5% oxygen. Contractions were induced in the strips by the addition of a suitable amount of histamine, acetylcholine or barium chloride. The amount of a given compound of Formula I (measured in pg/ml) required to provide greater than 75% relaxation of the drug induced contraction is considered an effective concentration. For comparison, a well known standard bronchodilator, aminophylline, requires concentrations of 50 pg/ml versus histamine, 100 pg/ml versus acetylcholine and 10 μg/ml versus barium chloride to provide greater than 75% relaxation of the drug induced contraction.

The compounds of Formula I may be administered to mammals in order to. obtain bronchodilation. The compounds may be administered orally, parenterally or by inhalation. The usual effective dose will be 0.1 to 50 mg/kg of body weight. Preferably, they are administered orally.

The compounds of Formula I, or their pharmaceutically acceptable acid-addition salts, can be combined with conventional pharmaceutically-acceptable diluents and carriers to form such dosage forms as tablets, capsules, suspensions, solutions, suppositories and the like to provide useful bronchodilator compositions.

The pharmaceutical carrier employed may be, for example, either a solid or liquid. Examples of solid carriers are lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid, and the like. Liquid carriers include syrup, peanut oil, olive oil, water and the like. Similarly, the carrier or diluent can include a time delay material well known to the art, such as glyceryl monostearate or glyceryl distearate, these being employed alone or, for example, in combination with a wax.

Some of the compounds of Formula I also have antiviral activity including:

1.8- dimethyl-a-fluoro-1H-imidazo[4,5-c]quinoline , l-methyl-4-(4-morpholino)-lH-imidazo[4,5-c]quinoline ,

1.8- dimethyl-lH-imidazo[4,5-c]quinoline ,

1.8- dimethyl-2-hydroxymethyl-lH-imidazo[4,5-c]quinoline ,

1- methyl-4-methoxy-lH-imidazo[4,5-c]quinoline,

2- (3-aminopropyl)-1,8-dimethyl-lH-imidazo[4,5-c]quinoline , N-(n-butyl)-l-methyl-lH-imidaza[4,5-c]quinolin-4-amine ,

1-(2,3-dihydroxypropyl)-N-methyl-lH-imidazo[4,5-c]quinolin4-amine ,

1- ethyl-2-me thyl-lH-imidazo[4,5-c]quinoline ,

2- benzylthio-l-methyl-lH-[4,5-c]quinoline , l-isobutyl-2-mercapto-lH-imidazo[4,5-c]quinoline ,

1-(2,3-dihydroxypropyl)-4-methoxy-lH-imidazo[4,5-c]quinoline, and

4-chloro-1-(4-me thoxyphenyl)-1H-imidazo[4,5-c]quinoline. The preferred antiviral compounds of Formula I are: 1,2-dimethy1-1H-imidazo[4,5-c]quinoline ,

1-benzy1-2-methy1-1H-imidazo[4-5c]quinoline, and

1,2,8-trimethyl-1H-imidazo[4,5-c]quinoline.

The antiviral activity of such compounds of Formula I is preferably demonstrated using the method described generally by Kern, et al., Antimicrob. Agents. Chemother. 14, 817-823 (1978) .

This method uses female guinea pigs of 200 to 300 grams in weight, preferably 200 to 250 grams in weight. The preferred strain of pigs is Hartley. The pigs are anesthetized with pentobarbital or methoxyflurane, and are then infected with about 10^ plaque forming units of Type II Herpes simplex virus type intravaginally using a cotton swab. Type I Herpes simplex virus may also be used in this screening method. Drugs are prepared in saline or in water using a surfactant such as Tween 80 (a polyoxyethylene sorbitan monooleate commercially available from Emulsion Engineering, Inc., Elk Grove Village, Illinois). Alternatively, the compounds of formula I may be formulated in PEG 400 (a polyethylene of average molecular weight of about 400, commercially available from Union Carbide Corporation) or in a polyethylene glycol cream. The drugs are applied intravaginally, for example, twice daily for a predetermined number of days, for example, five days. Application is initiated at a predetermined interval after infection such as one hour after infection. Virus replication can be monitored by determining the amount of virus recovered with vaginal swabs taken, for example, on days 1, 2, 3, 5 or 7 after infection. Virus is eluted from the swab in 1 ml of cell growth medium (Medium 199, Gibco Laboratories, Grand Island, New York) and virus titer is determined using cell monolayers. External lesions are scored daily for 10 days using the following scale: zero, no lesion; 1, redness or swelling; 2, a few small vesicles; 3, several large vesicles; 4, large ulcers and necrosis; 5, paralysis. Percent inhibition of lesion development is determined by comparing untreated, but infected control animals and drug treated animals. Comparison with known drugs such as phosphonacetic acid and acyclovir may also be undertaken.

For countering viral infection, active compounds of Formula I are used to control Type I or Type II Herpes simplex virus by applying to a population thereof an amount of a compound sufficient to attain said control. The application is preferably performed in vivo for treating infections caused by the viruses, especially in mammals. By active virus is meant non-dormant virus. The method is generally effective when a compound of the invention or its formulation is administered topically (e.g., intravaginally or on the skin), for example, to a genital herpes infection. With some compounds of Formula I, a genital herpes infection may also be treated by oral administration. The preferred route of administration of the compounds of Formula I is topical.

The antiviral compounds of Formula I are formulated for the various routes of administration in known, pharmaceutically acceptable vehicles such as water or polyethylene glycol, generally, the compound of Formula I being present in an amount of less than about 10% by weight, and preferably about 0.1-5% by weight. Such compounds of Formula I are preferably administered in water with either a surfactant such as Tween 80 discussed above or cellulose. A 5% concentration of the surfactant has been found to be generally useful in topical, oral and intraperitoneal formulations.

The following examples are provided to illustrate the invention and are not intended to be limiting thereof.

Example 1. Preparation of a Compound of Formula V To a stirred solution of 50.0g (0.24 mole) of

4-chloro-3-nitroquinoline in 300 ml of tetrahydrofuran was added, in small portions, 52.7g (0.72 mole) of isobutylamine. The mixture was heated at its reflux temperature for one hour and was then evaporated in vacuo. Water was added to the residue, and the solid was separated by filtration. The solid was suspended in one liter of water, and was dissolved by the gradual addition of concentrated hydrochloric acid (to pH 3 to 4) followed by filtration of the solution. The filtrate was basified (to pH 9 to 10) by the addition of concentrated ammonium hydroxide to provide bright yellow 4-(isobutylamino)-3-nitroquinoline, m.p. 119-121°C. The structural assignment was supported by infrared spectral analysis.

Example 2. Alternative Preparation of a Compound of Formula V

To a stirred solution of 40% aqueous methylamine was added, in small portions, 30.0g (0.144 mole) of

4-chloro-3-nitroquinoline. The reaction mixture was then heated at its reflux temperature for about 0.75 hour. After cooling, the mixture was poured in 300 ml of water. The solid was separated by filtration, and was then suspended in 300 ml of water. Acidification with 6N hydrochloric acid to pH 3 to 4 effected dissolution of most of the solid. Filtration was followed by basification of the filtrate with concentrated ammonium hydroxide to pH 3 to 10 to provide a yellow precipitate. The solid was separated by filtration, washed with water, and recrystallized from ethanol to provide yellow

4-methylamino-3-nitroquinoline, m.p. 168-170°C. Analysis: Calculated for C10H9N3O2: %C, 59.1; %H, 4.5; %N, 20.7; Found: %C, 59.0; %H, 4.2; %N, 20.8.

Using the methods of Examples 1 and 2, and starting with the indicated substituted quinolines and primary amines, the following compounds of Formula V were prepared (Table I):

<td colspan="4"> Table I</td>

<td> Ex. No.</td><td> Quinoline Starting Material of Formula IV</td><td> Primary Amine Starting Material</td><td> Intermediate of Formula V (m.p. in °C)</td>

<td> 3</td><td> 4,6-dichloro-3nitroquinoline</td><td> methylamine</td><td> 6-chloro-4-methylamino- 3-ni troquinoline (not taken)</td>

<td> 4</td><td> 4-chloro-3-nitroquinoline</td><td> ethanolamine</td><td> 4-(2-hydroxyethylamino)3-nitroquinoline (204-207)</td>

<td> 5</td><td> 4-chloro-3-nitro-</td><td> 2,3-dihydroxy-</td><td> 4-(2,3-dihydroxypropyl-</td>

<td></td><td> quinoline</td><td> propylamine</td><td> amino)-3-nitroquinoline (209-211)</td>

<td> 6</td><td> 4-chloro-3-ni troquinoline</td><td> ethylamine</td><td> 4-e thylamino-3-n i troquinoline (145-148)</td>

<td> 7</td><td> 4-chloro-6-me thyl3-ni troqui noli ne</td><td> methylamine</td><td> 6-methy1-4-methylamino3-nitroquinoline (168-171)</td>

<td> 8</td><td> 4-chloro-6-methyl- 3-nitroquinoline</td><td> isobutylamine</td><td> 4-isobutylamino-6-methyl- 3-nitroquinoline (108-110)</td>

<td> 9</td><td> 4-chloro-6-fluoro- 3-nitroquinoline</td><td> methylamine</td><td> 6-fluoro-4-methylamino3-nitroquinoline (198-202)</td>

<td> 10</td><td> 4,7-dichloro-3nitroquinoline</td><td> isobutylamine</td><td> 7-chloro-4-isobutylamino3-nitroquinoline (not taken)</td>

<td> 11</td><td> 4-chloro-3-nitroquinoline</td><td> aniline</td><td> 3-nitro-4-phenylaminoquinoline (129-132)</td>

<td> 12</td><td> 4-chloro-3-n itroquinoline</td><td> 4-methoxyaniline</td><td> 4-(4-me thoxyphenylamino)-3nitroquinoline (136-138)</td>

<td> 13</td><td> 4-chloro-3-nitroquinoline</td><td> 4-fluoroaniline</td><td> 4-(4-fluorophenylamino)-3nitroquinoline (147-151)</td>

<td> 14</td><td> 4-chloro-3-nitroquinoline</td><td> ammonia</td><td> 4-amino-3-nitroquinoline (263-265)</td>

<td> 15</td><td> 4-chloro-3-n i troquinoline</td><td> n-butylamine</td><td> 4-(n-butylamino)-3nitroquinoline (81-83)</td>

<td> 16</td><td> 4-chloro-3-ni tr0quinoline</td><td> 3-hydroxypropylamine</td><td> 4-(3-hydroxypropylamino)3-nitroquinoline (159-162)</td>

<td> 17</td><td> 4-chloro-6-fluoro -2-methy1-3-n i troquinoline</td><td> 2,3-dihydroxypropylamine</td><td> 4-(2,3-dihydroxypropyl- amino)-6-fluoro-2-methyl- 3-nitroquinoline (187-189)</td>

<td> 18</td><td> 4-chloro-6-fluoro- 2-me thyl-3-nitro quinoline</td><td> ammonia</td><td> 4-amino-6-fluoro-2-methyl 3-nitroquinoline (143-158)</td>

<td> 19</td><td> 4-chloro6־-fluoro-2- methyl-3-nitro- quinoline</td><td> methylamine</td><td> 6-fluoro-2-methy1-4me thylamino-3-n i troquinoline (182-184)</td>

<td> 20</td><td> 4-chloro-6-fluoro2-me thyl-3-n i troquinoline</td><td> benzylamine</td><td> 4-'oenzylamino-6-fluoro2-me thy1-3-ni troquinoline (171-174)</td>

<td> 21</td><td> 4-chloro-3-n i tr 0quinoline</td><td> 2- (N, N-dime thy 1amino)e thylamine</td><td> 4-[2-(N,N-dime thy1amino)ethylamino]-3nitroquinoline (124-145)</td>

<td> 22</td><td> 4-chloro-3-ni troquinoline</td><td> ethyl 4-aminophenylacetate</td><td> ethyl 4-(3'-nitro4’-quinolinyl)aminophenylacetate (104-106)</td>

<td> 23</td><td> 4-chloro-3-ni troquinoline</td><td colspan="2"> 4-c hlorobenzylamine 4-(4-chlorobenzy1amino)-3-nitroquinoline (not taken)</td>

<td> 24</td><td> 4-chloro-3-ni troquinoline</td><td colspan="2"> 2-me tho xye thylamine 4-{2-methoxye thylamino)3-nitroquinoline (115-118)</td>

<td> 25</td><td> 4-chloro-6-me thy13-nitroquinoline</td><td> n-butylamine</td><td> 4-(n-butylamino)-6methy1-3-nitroquinoline</td>

(not taken)

Example 26. Preparation of a Compound of Formula VI To a solution of 57.3g (0.23 mole) of

4-(isobutylamino)-3-nitroquinoline (from Example 1) in 600 ml of ethanol was added about 2g of platinum on charcoal, and the resulting mixture was hydrogenated on a Parr apparatus for three hours. Filtration followed by evaporation in vacuo provided a residue which gradually solidified to yellow solid 3-amino-4-(isobutylamino)quinoline .

Using the method of Example 26, and starting with the indicated intermediates of Formula V, the intermediates of Formula VI shown in Table II were prepared. In those cases where the hydrochloride is listed, it was obtained by first bubbling hydrogen chloride through an ethanol solution of the free amine and then separating the solid product by filtration.

<td></td><td> Table II</td>

Intermediate of

<td> Formula V (Example No.) 2</td><td> Intermediate of Formula VI (m.p. in ^aC) 3-amino-4-(methylamino ) quinoline hydrochloride (294-296)</td>

<td> 3</td><td> 3-amino-6-chloro-4-(methylamino)quinoline (not taken)</td>

<td> 4</td><td> 3-amino-4-(2-hydroxyethylamina)quinoline dihydrochloride (282-283)</td>

<td> 5</td><td> 3-amino-4-(2,3-dihydroxypropylamino)quinoline hydrochloride (201-204)</td>

<td> 6</td><td> 3-amino-4-(ethylamino)quinoline hydrochloride (226-229)</td>

<td> 7</td><td> 3-amino-6-methyl-4-(methylamino)quinoline hydrochloride (&gt;300)</td>

<td> 8</td><td> 3-amino-4-isobutylamino-6-methylquinoline (not taken)</td>

<td> 9</td><td> 3-amino-6-fluor0-4-(methylamino)quinoline (not taken)</td>

<td> 10</td><td> 3-amino-7-chloro-4-(isobutylamino quinoline (not taken)</td>

<td> 11</td><td> 3-amino-4-phenylaminoquin01ine (not taken)</td>

£4547/3

<td> 37</td><td> 12</td><td> 3-amino-4-(4-methoxyphenylamina)quinoline (not taken)</td>

<td> 38</td><td> 13</td><td> 3-amin0—4—(4—fluorophenylamino) — quinoline (not taken)</td>

<td> 39</td><td> 14</td><td> 3,4-diaminoquinoline (170-174)</td>

<td> 40</td><td> 15</td><td> 3-amin0-4-(n-butylamino) quinoline (80-83)</td>

<td> 41</td><td> 16</td><td> 3-amino-4-(3-hydroxypropylamino)quinoline (not taken)</td>

<td> 42</td><td> 17</td><td> 3-amino-4-(2,3-dihydroxypropylamino )-6-fluoro-2-methylquinoline (tan solid) (not taken)</td>

<td> 43</td><td> 18</td><td> 3,4-diamino-6-fluoro-2-methylquinoline (not taken)</td>

<td> 44</td><td> 19</td><td> 3-amino-6-fluoro-2-methyl-4(methylamindquinoline (123-131)</td>

<td> 45</td><td> 20</td><td> 3-amino-4-benzylamino-6-fluoro-2methylquinoline (not taken)</td>

<td> 4G</td><td> 21</td><td> 3-amino-4-[2-(N _׳ N-dimethylamina) ethylaminojquinoline (not taken)</td>

<td> 47</td><td> 22</td><td> ethyl 4-(3-amino-4-quinolinyl)aminophenylacetate (not taken)</td>

<td> 48</td><td> 22</td><td> 3-amino-4-(4-chlorobenzylamino)quinoline (not taken)</td>

Example 49. Preparation of a Compound of Formula VII Crude 3-amino-4-(methylaraino)quinoline (0.207 mole) obtained by the method of Example 26 was mixed with 500 ml of glacial acetic acid and 76 ml of triethyl orthoacetate, and the resulting mixture was heated at reflux for two hours. Evaporation provided a residue which was dissolved in 800 ml of water. The solution was basified with concentrated ammonium hydroxide. The solid was separated by filtration and washed with water to provide l,2-dimethyl-lH-imidazo[4,5-c]quinoline. When a sample of this product was recrystallized from diethyl ether, it had a melting point of 194-196°C. Analysis: Calculated for Ο]_2^11^ν3^: 73.1; %H, 5.6; %N, 21.3;

Found: %C, 73.4; %H, 5.7; %N, 21.5.

Using the method of Example 49, and starting with the indicated intermediates, carboxylic acids and trialkyl orthoesters, the compounds of Formula VII shown in Table III were prepared.

Table III

Intermediate Ortho Ester;

of Formula VI Carboxylic Compound of (Example No.)Acid Formula VII (m.p. in °C) triethyl l-isobutyl-lH-imidazo[4,5-c]quinoline orthoformate; (92-95) formic acid triethyl 8-chloro-l,2-dimethyl-lH-imidazo[4,5orthoacetate; cjquinoline (not taken) acetic acid triethyl 1-( 2-hydroxyethyl )-lH-imidazo[4,5-c]~ orthoformate; quinoline (170-172) formic acid triethyl l-(2,3-dihydroxypropyl)-2-methyl-lHorthoacetate; imidazo[4,5-c]quinoline (232-234) acetic acid triethyl l-ethyl-2-methyl-lH-imidazo[4,5-c]orthoacetate; quinoline (126-129) acetic acid triethyl l,8-dimethyl-lH-imidazo[4,5-c]orthoformate; quinoline hydrate (180-184) formic acid triethyl l,2,8-trimethyl-lH-imidazo[4,5-c]orthoacetate; quinoline (220-221) acetic acid triethyl l-ethyl-lH-imidazo[4,5-c]quinoline orthoformate; (80-82) formic acid

33 triethyl l-isobutyl-8-methyl-lH-imidazo[4,5- orthoformate; cjquinoline (160-163) formic acid

34 triethyl 8-fluoro-l-methyl-lH-imidazo[45_׳-c]- orthoformate; quinoline hydrate (201-205) formic acid

35 triethyl 7-chloro-l-isobutyl-lH-imidazo[4,5-c] orthoformate; quinoline (not taken) formic acid

36 triethyl l-phenyl-lH-imidazo[4,5-c]quinoline orthoformate; (137-139) formic acid

37 triethyl l-(4-methoxyphenyl)-lH-imidazo[4,5-c]- orthoformate; quinoline (150-152) formic acid

38 triethyl 1-( 4- fluorop henyl)-2-me thy 1-lH-imidazo- orthoacetate; [4,5-c]quinoline (191-193) acetic acid

37 triethyl l-(4-methoxyphenyl)-2-me thy 1-lH-imidazo- orthoacetate; [4,5-c]quinoline (174-176) acetic acid

38 triethyl l-(4-fluorophenyl)-lH-imidazo[4,5-c]- orthoformate; quinoline (159-161) formic acid

39 triethyl lH-imidazo[4,5-c]quinoline hydrate orthoformate; (>250) formic acid

<td> 67 40</td><td> tri ethyl 1-(n-butyl )-lH-imidazo[4,5-c]quinoline orthoformate; (not taken) formic acid</td>

<td> 68 41</td><td> triethyl 1- (3-hydroxypropyl )-lH-imidazo- orthoformate; [4,5-c]quinoline (not taken) formic acid</td>

<td> 69 27</td><td> triethyl l-methyl-lH-imidazo[4,5-c]quinoline orthoformate; (143-145) formic acid</td>

<td> 70 30</td><td> triethyl 1-(2,3-dihydroxypropyl)-lH-imidazo- orthoformate: [4,5-c]quinoline (228-230) formic acid</td>

<td> 71 26</td><td> triethyl l-isobutyl-2-methyl-lH-imidazo- orthoacetate; [4,5-c]quinoline hydrate acetic acid (85-88)</td>

<td> 72 34</td><td> triethyl l,2-dimethyl-8-fluoro-lH- orthoacetate; imidazo[4,5-c]quinoline acetic acid (234-239)</td>

<td> 73 47</td><td> triethyl ethyl 4-(l-lH-imidazo[4,5-c]- orthoformate; quinolinyl)phenylacetate formic acid (105-109)</td>

<td> Example 74.</td><td> Preparation of a Compound of Formula VIII.</td>

<td> To</td><td> a solution of 9.3g (0.0413 mole) of</td>

l-isobutyl-lH-imidazo[4,5-c]quinoline (from Example 57)־) in 150 ml of acetic acid was added 1.5 equivalents (0.062 mole) of 30% hydrogen peroxide. The mixture was heated at 65-70^eC for one day, and was then evaporated. The residue was neutralized with saturated sodium bicarbonate solution and the resulting mixture was extracted with dichloromethane. The extracts were dried, then evaporated to provide a residue which solidified gradually to yellow solid l-isobutyl-lH-imidazo[4,5-c]quinolin-5-oxide. This product was recrystallized twice from ethyl acetate to give a green solid, m.p. 211-213°C. Analysis: Calculated for ^c14^h15ⁿ3^{0: %c}ז⁷.⁶⁹ ׳ ^%H3.⁶ ׳i ^%N' ¹⁷.⁴ז Found: %C, 69.7; %H, 6.3: %N, 17.1.

Using the method of Example 74, and starting with the indicated intermediates, the compounds of Formula VIII shown in Table IV were prepared.

Table IV

Compound of

<td> Ex.</td><td> Formula VII</td><td> Compound of</td>

<td> No.</td><td> (Examole No.)</td><td> Formula VIII (m.p. in.’C)</td>

<td> 75</td><td> 51</td><td> 8-chloro-l, 2-dimethyl-lH־*imidazo[4,5-c]quinolin-5-oxide (not taken)</td>

<td> 76</td><td> 128</td><td> l-benzyl-lH-imidazo[4,5-c]quinolin-5-</td>

<td></td><td> (Part C)</td><td> oxide (241-251)</td>

<td> 77</td><td> 129</td><td> 1-cyclohexylmethy1-lH-imidazo[4,5-c]-</td>

<td></td><td> (Part C)</td><td> quinolin-5-oxide (224-226, dec.)</td>

<td> 78</td><td> 54</td><td> l-ethyl-2-methyl-lH-imidazo[4,5-c]quinolin-5-oxide (220-222)</td>

<td> 79</td><td> 55</td><td> 1,8-dimethy1-lH-imidazo[4,5-c]quinolin-</td>

<td></td><td></td><td> 5-oxide (265-268)</td>

<td> 80</td><td> 56</td><td> 1,2, a-trimethy1-lH-imidazo[4,5-c]quinolin-5-oxide (not taken)</td>

<td> 81</td><td> 57</td><td> 1-ethy1-lH-imidazo[4,5-c]quinolin-5oxide (not taken)</td>

<td> 82</td><td> 58</td><td> l-isobutyl-8-methyl-LH-imidazoC4,5-c]quinolin-5-oxide (not taken)</td>

<td> S3</td><td> 59</td><td> 8-fluoro-l-methyl-lH-imidazo[4,5-c]- quinolin-5-oxide (not taken)</td>

<td> 84</td><td> 60</td><td> 7-chloro-l-isobutyl-lH-imidazo[4/5-c]quinolin-5-oxide (not taken)</td>

<td> 85</td><td> 61</td><td> 1-phenyl-lH-iniidazo[ 4,5-c ]quinol in-5oxide (222-225)</td>

<td> 86</td><td> 62</td><td> 1-(4-methoxyphenyl)-1H-imidazo[4,5-c]quinolin-5-oxide (245-247)</td>

<td> 87</td><td> 63</td><td> 1-(4-fluorophenyl)-2-methyl-lH-imidazo[4,5-c]quinolin-5-0xide (245-248)</td>

<td> 88</td><td> 64</td><td> 1-(4-methoxyphenyl)-2-methyl-lH-imidazo[4,5-c]quinolin-5-oxide (211-213)</td>

<td> 89</td><td> 65</td><td> 1-(4-fluorophenyl)-lH-imidazo[4,5-c]quinolin-5-oxide (257-259)</td>

<td> 90</td><td> 66</td><td> 1H-imidazo[4,5-c]quinolin-5-oxide (not taken)</td>

<td> 91</td><td> 170</td><td> 2-methyl-1-[2-(phenyl) ethyl]-IH-imidazo[4,5-c]quinolin-5-oxide (204-206)</td>

<td> 92</td><td> 49</td><td> 1,2-dimethyl-lH-imidazo[4,5-c]quinolin5-oxide (234-237)</td>

<td> 93</td><td> 69</td><td> l-methyl-lH-imidazo[4,5-c]quinolin-5oxide (241-244)</td>

<td> 94</td><td> 73</td><td> ethyl 4-(l-lH-imidazo[4,5-c]quinolin-5-oxide)phenylacetate (not taken)</td>

l-isobutyl-2-methyl-lH-imidazo[4,5-c]quinolin-5-oxide (214-216)

1,2-dimethyl-8-fluoro-lH-imidazo[4,5-c]quinolin-5-oxide (not taken)

Example 97. Preparation of a Compound of Formula IX

A mixture of 9.95 g (0.0412 mole) of l-isobutyl-lH-imidazoC4,5-c]quinolin-5-oxide (from Example 74) and 100 ml of phosphorus oxychloride was heated at its reflux temperature for 2.5 hours, and was then cooled and poured into ice with stirring. Basification (to pH 9-10) with 50% aqueous sodium hydroxide solution was followed by extraction with dichloromethane. The extracts were dried over sodium chloride and sodium bicarbonate, and then evaporated to provide a solid residue. A sample of the residue was recrystallized from diethyl ether to provide

4-chloro-l-isobutyl-lH-imidazo[4,5-c]quinoline, m.p.

134-136^OC. Analysis: Calculated for Cj_4H^₄C1N3 : %C, 64.7: %H , 5.4; %N, 16.2; Found: %C, 64.3; %H, 5.3: %N, 16.3.

Using the method of Example 97, and starting with the indicated compounds of Formula VIII, the compounds of Formula IX were prepared.

<td colspan="3"> Table V</td>

<td> Ex. No. 98</td><td> Compound of Formula VIII (Example No.) 92</td><td> Compound of Formula IX (m.p. in °C) 4-chloro-1,2-dimethy1-lH-imidazo[4, 5-a]- quinoline (198-200)</td>

<td> 99</td><td> 75</td><td> 4,8-dichloro-l,2-dimethy1-1H-imidazo[4,5-c]quinoline (not taken)</td>

<td> 100</td><td> 76</td><td> l-benzyl-4-chlor0-1H-imidazo[4,5-c]quinoline (160-167)</td>

<td> 101</td><td> 77</td><td> 4-chloro-l-cyclohexylmethyl-lHimidazo[4,5-c]quinoline (176-179)</td>

<td> 102</td><td> 78</td><td> 4-chloro-l-e thy1-2-methy1-1H-imidazo- [4,5-c]quinoline (170-172)</td>

<td> 103</td><td> 79</td><td> 4-chloro-1,8-dime thy1-1H-imidazo[4,5-c]quinoline (233-237)</td>

<td> 104</td><td> 80</td><td> 4-chloro-1,2!8-trimethyl-lH-imidazo[4,5-c]quinoline (243-247)</td>

<td> 105</td><td> 81</td><td> 4-chloro-l-ethy1-lH-imidazo[4,5-a]quinoline (not taken)</td>

<td> 106</td><td> 82</td><td> 4-chloro-l-isobutyl-8-methy1-1H-imidazo[4,5-c]quinoline (202-205)</td>

<td> 107</td><td> 83</td><td> 4-chloro-8-fluoro-l-methyl-1H-imidazo-. [4,5-c]quinoline (not taken)</td>

<td> 108</td><td> 84</td><td> 4,7-dichloro-l-isobutyl-lH-imidazo[4,5c]quinoline (not taken)</td>

<td> 109</td><td>⁰⁵</td><td> 4-chloro-l-phenyl-lH-imidazo[4,5-c]quinoline (not taken)</td>

<td> 110</td><td> 86</td><td> 4-chloro-1-(4-methoxyphenyl)-lH-imidazo[4,5-c]quinoline (210-212)</td>

<td> 111</td><td> 87</td><td> 4-chloro-l-(4-fluorophenyl)-2-methy1-1Himidazo[4,5-c]quinoline (295-297)</td>

<td> 112</td><td> 88</td><td> 4-chlor0-1-(4-methoxyphenyl)-2-methy1-1Himidazo[4,5-c]quinoline (211-213)</td>

<td> 113</td><td> 89</td><td> 4-chloro-l-(4-fluorophenyl)-lH-imidazo[4,5-c]quinoline (248-250)</td>

<td> 114</td><td> 131, Part D</td><td> 4-chloro-l-[2-(phenyl)ethyl]-lH-imidazo[4,5-c]quinoline (176-188)</td>

<td> 115</td><td> 93</td><td> 4-chloro-l-methyl-lH-imidazo[4,5-c]quinoline (179-181)</td>

<td> 116</td><td> 165, Part B</td><td> l-benzyl-4-chloro-2-methy1-1Himidazo[4,5-c]quinoline (216-218)</td>

<td> 117</td><td> 95</td><td> 4-chlor0-1-isobuty1-2-methy1-1H-imidazo[4,5-c]quinoline (152-155)</td>

<td> 118</td><td> 96</td><td> 4-chloro-l, 2-dimethyl-8-fluoro-lH- imidazo-[4,5-c]quinoline (not taken)</td>

<td colspan="3"> Example 119 To a stirred, cold (5°C) mixture of 29.1 g (0.136 mole) of l-(2-hydroxyethyl)-lH-imidazo[4,5-c]quinoline (from Example 52) and 500 ml of pyridine was added, in small portions, 23.9 g (0.17 mole) of benzoyl chloride. The mixture was permitted to warm to about 20’C slowly, and was then stirred for eighteen hours at 20°C. The solution</td>

was evaporated, and water was added to the residue. The solid was separated by filtration, washed with water and recrystallized from a 50:50 ethyl acetate/hexane mixture.

Recrystallization from ethyl acetate and again from ethanol provided white crystals of

1-(2-benzoyloxyethyl)-lH-imidazoQ4,5-c]quinoline , m.p. 149-151°C. Analysis: Calculated for C19H15N3O2: %C, 71.9: %H, 4.8; %N, 13.2; Found: %C, 71.8: %H, 4.6; %N, 13.2.

Example 120

A mixture of 67.5 g (0.213 mole) of

1-(2-benzoyloxyethyl)-lH-imidazoC4,5-clquinoline ( from Example 119), 36.3 g (0.32 mole) of 30% hydrogen peroxide and 450 ml of glacial acetic acid was heated at 65°C for two days with stirring . The solution was then evaporated in vacuo, and the residue was added to water. The mixture was neutralized with aqueous sodium hydroxide solution and sodium bicarbonate. The solid was separated by filtration, washed with water and recrystallized from methanol to provide tan solid 1-(2-benzoyloxyethyl)-lH-imidazo[4,5-c]quinolin-5-oxide .

Example 121

A mixture of 50g (0.15 mole) of

1-(2-benzoyloxyethyl)-lH-imidazo[4,5-c]quinolin-5-oxide (from Example 120) and 200 ml of phosphorus oxychloride was heated for two hours on a steam bath. The mixture was then partially evaporated in vacuo. The mixture was then poured over ice and the solution was neutralized with sodium hydroxide. The product was separated by filtration, dissolved in dichloromethane, and the solution was washed with aqueous sodium bicarbonate solution and then dried. Evaporation provided a solid which was recrystallized from a 50:50 methanol:dichloromethane solution to provide white 1-(2-benzoyloxyethyl)-4-chloro-lH-imidazo[4,5-c]quinoline, m.p. 186-190°C. Analysis: Calculated for C19H2.4C1N3O2: %C,

64.9; %H, 4.0; %N, 12.0; Found: %C, 64.8; %H, 3.8; %N, 12.1.

Example 122 A mixture of 25.3 g (0.072 mole) of 1-(2-benzoyloxyethyl)-4-chloro-lH-imidazo[4,5-c]quinoline (from Example 121) and 500 ml of 10% ammonia in methanol was stirred at about 20°C for three days, and was filtered and then evaporated to low volume. The slurry was mixed with diethyl ether, and the solid was separated by filtration, washed with ether and recrystallized from methanol to provide white crystals of

4-chloro-l-(2-hydroxyethyl)-lH-imidazo[4,5-c]quinoline, m.p. 185-187°C. Analysis: Calculated for C12H10CIN3O: %C, 58.2: %H, 4.1: %N, 17.0: Found: %C, 58.0: %H, 4.0; %N, 17.3.

Example 123 To a solution of 3.0 g (0.013 mole) of l-isobutyl-lH-imidazo[4,5-c]quinoline (from Example 50) in 150 ml of ethanol was added hydrogen chloride gas. After stirring for about one hour the solid 1-isobutyl-lHimidazo[4,5-c]quinoline hydrochloride hydrate was separated by filtration and recrystallized from ethanol to provide off-white crystals, m.p. 227-229°C. Analysis: Calculated for C14H15N3.HC1.H₂O: %c,60.1: %H, 6.5: %H, 15.0; Found: %C, 60.2; %H, 6.2; %N, 15.4.

Example Part A

Using the method of Example 119, benzoyl chloride was reacted with 1-(2,3-dihydroxypropyl)-lH-imidazo[4,5-c]quinoline (from Example 70) to provide 1-(2,3-dibenzoyloxypropyl)-1H-imidazo[4,5-c]quinoline. Part B

The crude product from Part A was reacted with hydrogen peroxide according to the method of Example 120 to provide 1-(2,3-dibenzoyloxypropyl)-lH-imidazoC4,5-c]quinolin-5-oxide as a pale yellow solid, the melting point of crude material being 73-82°C.

Part C

The product from Part B was reacted with phosphorus oxychloride according to the method of Example 121 to provide 4-chloro-l-(2,3-dibenzoyloxypropyl)-lHimidazo[4,5-c]quinoline, m.p. 162-165°C after recrystallization from ethanol. Analysis: Calculated for C27H2QCIN3O4: %C, 66.7; %H, 4.1; %N, 8.6; Found: %C, 66.3; %H, 3.9; %N, 8.4.

Part D

Hydrolysis of the product from Part C according to the method of Example 122 provides 4-chloro-l-(2_t3dihydroxypropyl)-1H-imidazo[4,5-c]quinoline.

Example Part A 1-(2,3-Dihydroxypropyl)-lH-imidazo[4,5-c]quinoline (from Example 70) was reacted with excess acetic anydride to provide l-(2,3-diacetoxypropyl)-lH-imidazo[4,5-c]quinoline. Part B

The product of Part A was reacted with hydrogen peroxide according to the method of Example 120 to provide 1-(2/3-diacetoxypropyl)-lH-imidazoE4/5-c]quinoline-5-oxide as a brownish-yellow solid/ the melting point of the crude material being 84-96°C. Part C

The product of Part B was reacted with phosphorus oxychloride according to the method of Example 121 to provide 4-chloro-l-(2/3-diacetoxypropyl)-1H-imidazo[4,5-c]-quinoline. Part D

The product of Part C was hydrolyzed according to the method of Example 122 to provide 4-chloro-l-(2!3-dihydroxypropyl)-lH-imidazo[4/5-c]quinoline. Recrystallization from ethanol provided product/ m.p. 223-225°C. Analysis: Calculated for C]_3H]_2^C1^N3°2 ^: ^^c׳ 56.2/ %H1 4.4; %N, 15.1; Found: %C, 55.8, %H, 4.3; %N,

15.1.

Example 126

To a stirred solution of 4.0g (0.0117 mole) of 1-(2,3-diacetoxypropyl)-lH-imidazo[4,5-c]quinolin-5-oxide (from Example 125, Part B) in 50 ml of methanol was added about 12 drops of 25% sodium methoxide solution. After one hour the product was collected by filtration, washed with methanol and recrystallized from ethanol to provide 1-(2,3-dihydroxypropyl)-lH-imidazo[4,5-c]quinolin-5-oxide, m.p. 240-242°C. Analysis: Calculated for C13H13N3O3: %C, 60.2; %H, 5.1; %N, 16.2; Found: %C, 60.0; %H, 5.0; %N, 15.8.

Example 127

Excess acetic anhydride (100 ml) was refluxed for 0.5 hour with l-(2,3-dihydroxypropyl)-2-methyl-lH-imidazo[4,5-c]quinoline (from Example 53) to provide 1-(2,3-diacetoxypropyl)-2-methyl-lH-imidazoC4,5-c]quinoline . This product was reacted with hydrogen peroxide using the method of Example 120 to provide 1-(2,3-diacetoxypropyl)-2methyl-lH-imidazo[4,5-c]quinolin-5-oxide as a yellow solid. This crude. product was reacted with phosphorus oxychloride according to the method of Example 121 to provide the product 4-chloro-(2,3-diacetoxypropyl)-2-methyl-lH-imidazo[4,5-c]quinoline. This product was dissolved in methanol saturated with ammonia, and the solution was stirred for three days. The product obtained was 4-chloro-l-(2,3dihydroxypropyl)-2-methyl-lH-imidazo[4,5-c]quinoline.

Example Part A

Using the method of Example 1, benzylamine and

4-chloro-3-nitroquinoline were reacted to provide

4-benzylamino-3-nitroquinoline. The structural assignment for the crude product (m.p. 178-196°C) was supported by infrared spectral analysis.

Part B

Using the method of Example 26, 42.2g (0.15 mole) of 4-benzylamino-3-nitroquinoline was reduced to provide

3- amino-4-(benzylamino)quinoline as a tan solid. Part C

To the product from Part B was added 48.7g (0.5 mole) of diethoxymethyl acetate and the mixture was heated on a steam bath for one hour, and was then maintained at reflux for 0.5 hour. The solution was added to a stirred excess of concentrated ammonium hydroxide. The solid was separated by filtration and washed sequentially with water, 10:1 diethyl ether: ethanol and 1:1 hexane:diethyl ether. Recrystallization from isopropanol provided pale yellow needles of l-benzyl-lH-imidazo[4,5-c]quinoline, m.p. 179-181°C. Analysis: Calculated for C3.7Hj.3N3: %C, 78.7; %H, 5.1; %N, 16.2; Found: %C, 78.6; %H, 4.8; %N, 16.3.

Example Part A A mixture of 26.1g (0.125 mole) of

4- chloro-3-nitroquinoline, 16.4g (0.1275 mole) of 95% cyclohexylmethylamine and 16.5 g (0.125 mole) of 95% diisopropyl ethylamine in 300 ml of tetrahydrofuran was heated on a steam bath for 0.5 hour. The solution was evaporated and the residue was slurried in methanol, filtered and washed with methanol. Recrystallization from methanol provided yellow platelets of 4-ayclohexylmethylamino-3-nitroquinoline, m.p. 140-142°C. Analysis: Calculated for C15H19N3O2: %C, 67.3; %H, 6.7; %N, 14.7; Found: %C, 67.3; %H, 6.6; %N, 14.7.

Part B

Using the method of Example 26, 17 g (0.60 mole) of 4-cyclohexylmethylamino-3-nitroquinoline was reduced to provide 3-amino-4-cyclohexylmethylaminoquinoline. Part C

The crude product from Part B was heated at reflux for 2.5 hours in 250 ml of 98% formic acid to provide 1-cyclohexylmethyl-lH-imidazo[4,5-c]quinoline as a pale yellow solid.

Example 130

Using the method of Example 1, 4-chloro-3-nitroquinoline was reacted with 4-chlorobenzylamine to provide yellow solid 4-(4-chlorobenzylamino)-3-nitroquinoline/ melting point of crude product 168-173^eC.

Example Part A

Using the method of Example 1,

4- chloro-3-nitroquinoline was reacted with

2- (phenyl)ethylamine to provide yellow solid

3- nitro-4-[2-(phenyl)ethylamino]quinoline, the melting point of the crude product being 174-180°C.

Part B

Using the method of Example 26,

3-nitro-4-[2-(phenyl)ethylamino]quinoline from Part A was reduced to provide 3-amino-4-[2-(phenyl)ethylamino]quinoline. Part C

Using the method of Example 49, 3-amino-4-[2(phenyl)ethylamino]quinoline was reacted with triethyl orthoformate and formic acid to provide l-[2-(phenyl)ethyl]-lH-imidazo[4,5-c]quinoline , m.p. 105-108°C. Part D

Using the method of Example 74, 1-[2-( phenyl)ethylamino]-lH-imidazo[4,5-c]quinoline was converted to yellow solid l-[2-(phenyl)ethyl]-lH-imidazoC4,5-c]quinolin-

5- oxide, melting point of crude product, 73-95°C.

Example 132

To a solution of 4.0g (0.0155 mole) of l-isobutyl-2-mercapto-lH-imidazo[4,5-c]quinoline ( from Example 165, Part B) in 40 ml of methanol was added 3.7 g of 25% sodium methoxide in methanol, followed by the addition of 2.4 g (0.0171 mole) of methyl iodide. The solution was heated on a steam bath for 0.5 hour, and was then evaporated. Water was added to the residue and the mixture was extracted with dichlororaethane. The extracts were washed with water, dried over sodium chloride and evaporated. The residue was dissolved in diethyl ether and the mixture was saturated with hydrogen chloride. The precipitate was separated by filtration, washed with ether, and recrystallized from a mixture of ethanol and ether to provide l-isobutyl-2-methylthio-lH-imidazo[4,5-c]quinoline hydrochloride, m.p. 214-216°C. Analysis: Calculated for ^c15^h17ⁿ3^s,HC1: %C, 58.5; %H, 5.9; %N, 13.7; Found: %C,

57.9; %H, 5.7; %N, 13.7.

Example 133

A sample of 2-(3-aminopropyl)-1,8-dimethyl-lHimidazo[4,5-c]quinoline dihydrochloride (from Example 148) was dissolved in water. Excess sodium hydroxide was added to neutralize the hydrochloric acid and then excess acetic anhydride was added. The precipitate was separated by filtration, washed with water, and recrystallized from water to provide 2-(3-acetamidopropyl)-1,8-dimethyllH-imidazo[4,5-c]quinoline,m.p. 213-215°C. Analysis: Calculated for C17H20N4O: %C, 63.9; %H, 6.8; %N, 18.9;

Found: %C, 68.8; %H, 6.8; %N, 19.0.

Example 134 A mixture of 2.7g (0.0080 mole) of 1-(2,3-diace toxypropyl)-lH-imidazoC4,5-c]quinolin-5-oxide (from Example 125, Part B) and 50 ml of acetic anhydride was heated at its reflux temperature for one hour. The solution was evaporated and the residue was mixed with 65 ml of methanol. The mixture was basified (to pH 9-10) with 25% sodium methoxide in methanol. The precipitate was separated by filtration, washed with methanol and recrystallized twice from methanol. The product was 1-(2,3-dihydroxypropyl)-4-hydroxy-lH-imidazo[4,5-c]quinoline hydrate, m.p. 214-217°C. Analysis: Calculated for

C13H13N3°3:°-^50h20: %C, 58.2; %H, 5.3; %N, 15.7; Found: %C,

57.7; %H, 4.9; %N, 15.5.

Example 135

Using the method of Example 134, 1,2-dimethyllH-imidazo[4,5-c]quinolin-5-oxide (from Example 92) was reacted with acetic anhydride to provide l, 2-dimethyl-4-hydroxy-lH-imidazo[4,5-a]quinoline , m.p.

>300״C. Analysis: Calculated for C12H11N3O: %C, 67.7;

%H, 5.2; %N, 19.7; Found: %C, 67.1; %H, 5.1; %N, 19.5.

Example 136

Using the method of Example 134,

1-(4-methoxyphenyl)-1H-imidazo[4,5-c]quinolin-5-oxide ( from Exmaple 86) was reacted with acetic anhydride to provide

4-hydroxy-l-(4-methoxyphenyl)-lH~imidazo[4,5-c]quinoline

m. p. >300°C after recrystallization from Ν,Ν-dimethylformamide. Analysis: Calculated for ^C17^H13^N3°2^{: %c}70.1 ׳; %H, 4.5; %N , 14.4; Found: %C, 70.0; %H, 4.4; %N, 14.5.

Example 137

Using the method of Example 134,

1-(2-hydroxyethyl)-lH-imidazo[4,5-c]quinolin-5-oxide prepared by hydrolysis of the compound of Example 120 was reacted with acetic anhydride to provide

4-hydroxy-l-(2-hydroxye thyl)-1H-imidazo[4,5-c]quinoline.

The compound 4-hydroxy-l-(2-hydroxyethyl)-1H[4,5-c]quinoline was found to have m.p. >300°C after recrystallization from Ν,Ν-dimethylformamide. Analysis: Calculated for C12H11N3O2: %C, 62.9; %H, 4.8; %N, 18.7;

Found: %C, 62.7; %H, 4.7; %N, 18.3.

Example 138

A mixture of 2.2g (0.0115) of 3,4-diamino-6fluoro-2-methylquinoline (from Example 43) and 50 ml of 95% formic acid was heated at its reflux temperature for two hours, and was then evaporated. Water (100 ml) was added to the residue, and the mixture was basified with 50% aqueous sodium hydroxide solution to pH 9 to 10. The precipitate formed was separated by filtration and washed with water. Recrystallization from ethanol provided white solid 8-fluoro-4-methyl-lH-imidazo[4,5-c]quinoline hydrate, m.p. >250°C. Analysis: Calculated for Cj]_HgFN3 .H20: %C,

60.3; %H, 4.6; %N, 19.2; Found: %C, 60.1; %H, 4.7; %N,

18.5.

Example 139

Using the method of Example 138, 3-amino-4-(2,3dihydroxypropylamino)-6-fluoro-2-methy!quinoline ( from Example 42) was reacted with formic acid to provide 1-(2,3-dihydroxypropyl)-8-fluoro-4-methyl-lH-imidazo[4,5-c]quinoline hydrate, m.p. 237-239°C. Analysis: Calculated for C14H14FN3O2.H20: %C, 57.3; %H, 5.5; %N, 14.3; Found: %C, 57.6; %H, 5.4; %N, 14.4.

Example 140

Using the method of Example 138,

3-amino-4-benzylamino-6-fluoro-2-methylquinoline ( from Example 45) was reacted with formic acid to provide

1- benzyl-S-fluoro-4-me thyl-lH-imidazo[4,5-c]quinoline hydrate, m.p. 178-181°C. Analysis: Calculated for C₁₃H₁4FN30.25־H20: %C, 73.1; %H, 4.9; %N, 14.2; Found: %C, 73.0; %H, 4.7; %N, 14.3.

Example 141

Using the method of Example 138, 3-amino-6-fluoro-

2- methyl-4-methylaminoquinoline (from example 44) was reacted with formic acid to provide

1,4-dimethy1-8-fluoro-lH-imidazo[4,5-c]quinoline , m.p. 184-186°C. Analysis: Calculated for C!2^H10^FN3^: %^c67.0 ׳; %H, 4.7; %N, 19.5; Found: %C, 66.6; %H, 4.4; %N, 19.7.

Out of ambit

Example 142

Using the method of Example 138, 3-amino-4-[2(N,N-dimethylamino)ethylamino]quinoline (from Example 46) was reacted with formic acid to provide l-[2-(N, N-dimethylamino)ethyl]-lH-imidazo[4,5-c]quinoline . The product was dissolved in ethanol and hydrogen chloride was bubbled into, the solution. The precipitate was separated by filtration, washed with ethanol and recrystallized from ethanol. The product was 1-[ 2־־(N, N-dimethylamino) ethyl ]-lH-imidazo[4,5-c ]quinoline trihydrochloride hydrate, m.p. >250°C. Analysis: Calculated for 0]_4Η]_θΝ4.3Η01.Η20: %C, 45.8; %H, 5.5; %N, 15.3; Found: %C, 46.0; %H, 5.2; %N, 15.5.

Example 143

Using the method of Example 1,

4-chloro-3-nitroquinoline was reacted with 4-aminophenylacetic acid in Ν,Ν-dimethylformamide in the presence of triethylamine to provide N-(3-nitro-4-quinolinyl)-4-aminophenylacetic acid. This acid was reduced using the method of Example 26 to provide N-(3-amino-4-quinolinyl)-4-aminophenylacetic acid. This diamine was then reacted with formic acid using the method of Example 136 to provide 1-(4-carboxymethylphenyl)-lH-imidazo[4,5-c]quinoline. Recrystallization from methanol provided solid of m.p. 236-240°C. Analysis: Calculated for C1BH13N3O2: %C, 71.3; %H, 4.3; %N, 13.9; Found; %C, 70.8; %H, 4.3; %N, 13.7.

Example 144

A mixture of 4.5 g (0.020 mole) of

3-amino-6-methyl-4-(methylamino)quinoline hydrochloride (from Example 32), 3.8g (0.050 mole) of glycolic acid and 75 ml of 4N hydrochloric acid was heated at its reflux temperature for two hours. The solution was cooled, and 50% aqueous sodium hydroxide was then added to make the solution slightly basic. The precipitate was separated by filtration and washed with water. The solid was redissolved in dilute hydrochloric acid and reprecipitated with ammonium hydroxide to provide

1.8- dimethyl-2-hydroxymethy1-1H-imidazo[4,5-c]quinoline hydrochloride hydrate. Analysis: Calculated for ^C13^H13^N3°*^HC1*^H2^O: 55.4; %H, 5.7; %N, 14.9; Found: %C,

55.2; %H, 5.6; %N, 15.5.

Example 145

A mixture of 4.5g (0.0201 mole) of

3-amino-6-methyl-4-(methylamino)quinoline hydrochloride (from Example 32), 9.1g (0.080 mole) of trifluoroacetic acid and 100 ml of 4N hydrochloric acid was heated at its reflux temperature for three hours. The solution was cooled and basified with ammonium hydroxide. The precipitate was separated by filtration and washed with water. Recrystallization from isopropanol provided

1.8- dimethy1-2-trifluoromethyl-lH-imidazo[4,5-c]quinoline, m.p. 220-223°C. Analysis: Calculated for C13H10F3N3: %C, 58.9; %H, 3.8; %N, 15.8; Found: %C, 58.6; %H, 3.7; %N,

16.2.

Example 146

Using the method of Example 145, 3,4-diaminoquinoline (from Example 39) was reacted with trifluoroacetic acid to provide

2-trifluoromethy1-lH-imidazo[4,5-c]quinoline! m.p. 252-254⁰C. Analysis: Calculated for C11H5F3N3: %C, 55.7; %H, 2.5; %N, 17.7; Found: %C, 55.3; %H, 2.3; %N, 18.2.

Out of ambit

Example 147

To a solution of 6.6g (0.041 mole) of

3,4-diaminoquinoline (from Example 39), 2.0 ml of glacial acetic acid, 35 cc of ethanol and 35 ml of water was added 9.3g (0.045 mole) of N-carbomethoxy-S-methylisothiourea, and the mixture was heated at its reflux temperature for two hours. Evaporation provided a residue which was suspended in ethanol, separated by filtration and washed

Out of ambit with water. Recrystallization from ethanol provided methyl lH-imidazo[4,5-c]quinolin-2-carbamate hydrate, m.p. >250°C.

Analysis: Calculated for 012^10^402.0-75H20: %c, 56.4; %H,

£.5; %N, 21.9; Found: %C, 56.1; %H, 4.4; %N, 22.4.

Example 148

A mixture of 5.8 g (0.026 mole) of

3-amino-6-methyl-4-(methylamino)quinoline (the hydrochloride salt of which having been obtained in Example 32), 4.1g (0.040 mole) of 4-aminobutyric acid and 100 ml of 4N hydrochloric acid was heated at its reflux temperature for about 65 hours. The solution was cooled and diluted to 500 ml total volume with isopropanol. The precipitate was separated by filtration, and then recrystallized from aqueous isopropanol to provide yellow crystals of

2-(3-aminopropyl)-1,8-dimethyl-lH-imidazo[4,5-c]quinoline dihydrochloride, m.p. >300°C. Analysis: Calculated for

C]_5^h1₈N4*2HC1: %C, 55.0; %H, 6.2; %N, 17.1; Found: %C, 54.3; %H, 6.2: %N, 17.1.

Example 149 □sing the method of Example 148, 3,4-diaminoquinoline (from Example 39) was reacted with glacial acetic acid to provide 2-methyl-lH-imidazo[4,5-c]quinoline as a white solid, crude m.p. 119-123°C.

Example 150

Using the method of Example 148, 3-amino-4(methylamino)quinoline (the hydrochloride salt of which having been obtained in Exmple 27) was reacted with isobutyric acid to provide 2-isopropyl-l-methyllH-imidazo[4,5-c]quinoline. The crude product was dissolved in ethyl acetate and an excess of concentrated hydrochloric acid was added. The precipitate was separated by filtration and recrystallized from ethanol to provide

2-isopropyl-l-methy1-lH-imidazo[4,5-c]quinoline hydrochloride, m.p. 260-263’C. This salt was suspended in water and the mixture was basified (pH 8-10) with 50% aqueous sodium hydroxide. The solid was separated by filtration, washed with water and recrystallized from hexane to provide the free base as the hydrate, m.p. 76-81^eC. Analysis: Calculated for C14H15N3•0.25H20: %C, 73.2; %H, 6.8; %N, 18.3; Found: %C, 73.0 %H, 7.0; %N, 18.4.

Example 151

Using the method of Example 74, 1,4-dimethyl-8fluoro-lH-imidazo[4,5-c]quinoline (from Example 141) was reacted with hydrogen peroxide to provide l, 4-dimethy1-8-fluor0-1H-imidazo[4,5-c]quinolin-5-oxide ,

m. p. 245-248°C. Analysis: Calculated for C12^H10^FN3^0: %^c' 62.3; %H, 4.4; %N, 18.2; Found: %C, 62.7; %H, 4.3; %N,

18.3.

Example 152

A mixture of 2.0g (0.0068 mole) of l-benzyl-4-chloro-lH-imidazo[4,5-c]quinoline (from Example 100) and 25 ml of morpholine was heated at its reflux temperature for one hour. The solution was evaporated, and 30 ml of water was added to the residue. The solid which did not dissolve was separated by filtration, washed with water and recrystallized from ethanol. The product obtained was l-benzyl-4-(4-morpholino)-lH-imidazo[4,5-c]quinoline hydrate, m.p. 160-162°C. Analysis: Calculated for 021Η20Ν₄0.0.25Η2θ: %C, 72.3; %H, 5.9; %N, 16.1; Found: %C, 72.1; %H, 5.8; %N , 16.0.

Using the general method exemplified in Example 152, and starting with morpholine and the indicated intermediate of Formula IX, compounds of the invention of Formula X shown in Table VI were prepared.

־

TABLE VI

<td></td><td> Intermediate of</td><td></td>

<td> Ex.</td><td> Formula IX</td><td> Product of Formula X</td>

<td> No.</td><td> (Example No.)</td><td> (melting point in °C)</td>

<td> 153</td><td> 115</td><td> 1-me thyl-4-(4-morpholino)-1Himidazo-[4,5-c]quinoline (207-209</td>

<td> 154</td><td> 103</td><td> 1,8-dimethy1-4-(4-morpholino)-1H-</td>

imidazo[4,5-c]quinoline (250-256)

Example 155

A mixture of 40% aqueous methylamine (25 ml) and 5.0 g (0.023 mole) of 4-chloro-l-methyl-lH-imidazo[4,5-c]quinoline (from Example 115) was placed in a metal pressure reactor and heated at 112°C for about 16 hours. After cooling, the solid was separated by filtration, washed with water, dried and recrystallized from ethanol to provide N,1-dimethyl-lH-imidazo[4,5-c]quinolin-4-amine, m.p. 216-218’C. Analysis: Calculated for Cq2^H12^N4^{: %c}' ^7.^9:%H, 5.7; %N, 26.4; Found: %C, 67.9; %H, 5.6; %N, 26.4.

Using the method of Example 155, the following compounds of Examples 156 and 157 were prepared:

Example 156

N,N,l-trimethyl-lH-imidazo[4,5-c]quinolin-4-amine (m.p. 162-164°C)

Example 157

1-(2,3-dihydroxypropyl)-N-methyl-lH-imidazo[4,5-c]quinolin-4-amine (m.p. 201-203’C).

Example 158

A mixture of 3.6 g (0.0116 mole) of 4-chloro-l-(4methoxyphenyl)-lH-imidazo[4,5-c]quinoline (from Example 110), 25.1g (0.116 mole) of 25% sodium methoxide in methanol and 50 ml of methanol was heated its reflux temperature for one hour. Evaporation provided a residue which was diluted with 75 ml of water. The precipitate was separated by filtration, washed with water and recrystallized from ethanol to provide

4-methoxy-l-(4-methoxyphenyl)-1H-imidazo[4,5-c]quinoline , m.p. 180-182°C. Analysis: Calculated for C!8^H15^N3°2^: ׳

70.8; %H, 5.0; %N, 13.8; Found: %C, 70.6; %H, 5.0; %N, 13.9.

Example 159

Using the method of Example 158, 4-chloro-lmethyl-lH-imidazo[4,5-c]quinoline (from Example 115) was reacted with sodium methoxide to provide

4-methoxy-l-methy1-lH-imidazo[4,5-c]quinoline, melting point after recrystallization from ethyl acetate 160-162°C. Analysis: Calculated for C!2^H11^N3^{O: %c}67.6 ׳; %H, 5.2; %N, 19.7; Found: %C, 67.3; %H, 5.0; %N, 19.8.

Example 160

Using the method of Example 158, 4-chloro-l-(2,3dihydroxypropyl)-lH-imidazo[4,5-c]quinoline (from Example 125, Part D) was reacted with sodium methoxide to provide 1-(2,3-dihydroxypropyl)-4-methoxy-lH-imidazo[4,5-c]quinoline, m.p. 214-216°C after recrystallization from isopropanol. Analysis: Calculated for Cj_4H]_5N303: %C, 61.5; %H, 5.5; %N, 15.4; Found: %C, 61.3; %H, 5.5; %N, 15.4.

Example 161

To a mixture of 24.75g (0.1145 mole) of 25% sodium methoxide in methanol and 100 ml of ethanol was added 8.5g (0.1374 mole) of ethanethiol, followed by the addition of 5.0g (0.0229 mole) of 4-chloro-l-methy1-lH-imidazo[4,5-c]quinoline (from Example 115). The mixture was heated at its reflux temperature for one hour, and was then evaporated. Water was added to the residue and the solid obtained was separated by filtration and washed with water. Recrystallization from ethyl acetate provided yellow crystals of 4-ethylthio-l-methyl-lH-imidazo[4,5-c]quinoline, m.p. 112-115°C.' Analysis: Calculated for

C13H13N3S: %C, 64.2; %H, 5.4; %N, 17.3; Found: %C, 64.4;

%H, 5.3; %N, 17.6.

Example 162

Using the general procedure of Example 161, and substituting thiophenol for ethanethiol, 4-chloro-l-methyllH-imidazo[4,5-c]quinoline (from Example 115) was converted to l-methyl-4-phenylthio-lH-imidazo[4,5-c]quinoline, m.p. 213-215°C after recrystallization from ethyl acetate. Analysis: Calculated for C17H13N3S: %C, 70.1; %H, 4.5; %N, 14.4; Found; %C, 69.8; %H, 4.3; %N, 14.7.

Example 163

To a solution of 4.4g (0.071 mole) of

1- isobutyl-2-mercapto-lH-imidazo[4,5-c]quinoline ( from Example 165, Part B below) in 45 ml of methanol and was added 4.1g (0.0188) of 25% sodium methoxide in methanol, then 2.4g (0.0188 mole) of benzyl chloride. The solution was heated at reflux for 0.5 hour, then evaporated. Water was added to the residue, and the mixture was extracted with dichloromethane. The extracts were dried over sodium chloride, and then evaporated. The residue was dissolved in diethyl ether, and the solution was saturated with hydrogen chloride. The precipitate was separated by filtration, washed with ether and recrystallized from a mixture of ethanol and diethyl ether to provide

2- benzylthio-l-isobutyl-LH-imidazo[4,5-a]quinoline hydrochloride, m.p. 205-207°C. Analysis: Calculated for C21^H2!N3S*HC1: %C, 65.7; %H, 5.8; %N, 10.9; Found: %C, 65.4; %H, 5.6; %N, 10.9.

Example 164

Using the method of Example 163, 2-mercapto-l-methyllH-imidazo[4,5-c]quinoline (from Example 166 below) was reacted with benzyl chloride to provide _ 49 -

2- benzylthio-1-me thyl-lH-imidazo[4,5-c]quinoline . Recrystallization first fro'm isopropanol then from ethanol provided solid product, m.p. 160-163°C. Analysis: Calculated for C18H15N3S: %C, 70.8; %H, 5.0; %N, 13.8. Found: %C, 70.3; %H, 4.7; %N, 13.7.

Example Part A

To a solution of 15.0g (0.0612 mole) of

4-isobutylamino-3-nitroquinoline (from Example 1) in ethanol was added about 0.5g of 5% platinum on charcoal, and the mixture was hydrogenated on a Parr apparatus at about 20°C. The mixture was filtered to provide a solution of 3-amino-4-(isobutylamino)quinoline. Part B

To the solution from Part A was added first 10 ml of carbon disulfide, and then 4.6g (0.07 mole) of 85% potassium hydroxide. The solution was heated on a steam bath for two hours, and was evaporated to near dryness. The residue was dissolved in water, the solution acidified to pH 5 to 6 with glacial acetic acid and the precipitate separated by filtration and washed with water. Recrystallization from ethanol provided yellow

1- isobuty1-2-mercapto-lH-imidazo[4,5-c]quinoline, m.p. >300°C. Analysis: Calculated for C14H15N3S: %C, 65.3; %H, 5.9; %N, 16.3; Found: %C, 64.8; %H, 5.7; %N, 16.3.

Example 166

Using the method of Example 165, 4-methylamino-

3- nitroquinoline (from Example 2) was converted to

2- mercapto-l-methyl-lH-imidazo[4,5-c]quinoline.

Example Part A

Using the method of Example 49,

3- amino-4-(benzylamino) quinoline (from Example 128, Part B), was reacted with triethyl orthoacetate and acetic acid to provide l-benzyl-2-methyl-lH-imidazo[4,5-c]quinoline hydrate, m.p. 145-147°C. Analysis: Calculated for

C18H15N3.2.25H2O: %C, 68.9; %H, 6.3, %N, 13.4; Found: %C,

69.2; %H, 6.0; %N 13.4.

Part B

Using the method of Example 74, l-benzyl-2-methyl-lH-imidazo[4,5-c]quinoline was converted to l-benzyl-2-methyl-lH-imidazo[4,5-c]quinolin-5-oxide hydrate, m.p. 193-196’C. Analysis: Calculated for C18H15N3O.2.25H2O: %C, 65.6; %H, 6.0; %N, 12.7; Found: %C, 65.4; %H, 5.7; %N, 12.5.

Example 168

To a solution of 5.7 g (0.30 mole) of 3-hydroxy-

4-nitroquinoline in 50 ml of Ν,Ν-dimethylformamide was added 9.3 g (0.60 mole) of phosphorus oxychloride. The solution was heated on a steam bath for 5 minutes, then poured with stirring into 200 ml of 40% aqueous methylamine. The mixture was heated on a steam bath for fifteen minutes, then diluted with 200 ml of water. The solid was separated by filtration, then dissolved in dilute hydrochloric acid. The solution was filtered and the filtrate was basified with ammonium hydroxide. The solid precipitate was separated by filtration, washed with water and dried to provide yellow solid 4-methylamino-3-nitroquinoline, m.p. 167-171°C.

Example 169

To a solution of 4.8 g (0.0311 mole) of phosphorus oxychloride in 20 ml of Ν,Ν-dimethylformamide was added, in small portions, 5.0 g (0.207 mole) of l-isobutyl-lH-imidazo[4,5-c]quinoline-5-oxide. The solution was stirred for 15 minutes at 20°C, then heated on a steam bath for 15 minutes. The solution was cooled to 20°C, then poured into stirred ice. The solution was basified to pH 8 with concentrated ammonium hydroxide. The yellow solid precipitate was separated by filtration.

J ־-־ Ο washed sequentially with water and diethyl ether, and dried to provide 4-chloro-l-isobutyl-lH-imidazo[4,5-c]quinoline hydrate, m.p. 103-107°C. Recrystallization twice from ethyl acetate with drying provided 4-chloro-l-isobutyl-lHimidazo[4,5-c]quinoline, m.p. 135-137°C. Analysis: Calculated for C14H14CIN3: %C, 64.7; %H, 5.4; %N, 16.2; Found: %C, 64.6; %H, 5.5; %N, 16.1.

Example 170

Using the method of Example 49, 3-amino-4-[2(phenyl)ethylamino]quinoline (from Example 131, Part B) was reacted with triethyl orthoacetate and acetic acid to provide 2-methy1-1-[2-(phenyl)ethyl]-lH-imidazo[4,5-c]quinoline.

Example 171

Using the method of Example 158, 4-chloro-lisobutyl-lH-imidazo[4,5-c]quinoline (from Example 97) was reacted with sodium methoxide to provide l-isobutyl-4methoxy-lH-imidazo[4,5-c]quinoline, melting point 111-114°C after sequential recrystallizations from aqueous ethanol and diethyl ether. Analysis: Calculated for C15H17N3O: %C, 70.6; %H, 6.7; %N, 16.5; Found: %C, 70.6; %H, 6.7; %N,

16.5.

Example 172

Using the method of Example 134, 1-isobutyl-lHimidazo[4,5-c]quinolin-5-oxide (from Example 74) was reacted with acetic anhydride to provide 4-hydroxy-lisobutyl-lH-imidazo[4,5-c]quinoline, m.p. >300°C after recrystallization from N,N-dimethylformamide. Analysis: Calculated for C14H15N3O: %C, 69.7; %H, 6.3; %N, 17.4; Found: %C, 69.8; %H, 6.4; %N, 17.6.

Example Part A

Using the method of Example 26,

4-(4-chlorobenzylamino)-3-nitroquinoline (from Example 23) was reduced to provide 3-amino-4-(4-chlorobenzylamino)quinoline.

Part B

The product from Part A was reacted with triethyl orthoacetate and acetic acid using the method of Example 49 to provide l-(4-chlorobenzyl)-2-methyl-lH-imidazo[4,5-c]quinoline, m.p. 183-185^OC.

Example 174

Using the general method exemplified in Example 152, 4-chloro-l-methyl-lH-imidazo[4,5-c]quinoline (from Example 115) was reacted with jn-butylamine to provide N-butyl-l-methyl-lH-imidazo[4,5-c]quinolin-4-amine, m.p. 98-100’C.

Example

Step (1)

To a solution of 22.5 g (0.0823 mole) of 4_(_n-hexyl)amino-3-nitroquinoline in 300 ml of toluene was added about 1.0 g of 5% platinum on charcoal and the mixture was hydrogenated on a Paar apparatus for 1.5 hours. Filtration followed by evaporation in vacuo provided a residue of 3-amino-4-(n-hexyl)aminoquinoline as an orange solid. Thin layer chromatographic analysis of the product on silica gel, eluting with methanol, showed one spot at Rf=0.73 and a trace at Rf=0.35. Step (2)

The crude reaction product obtained by the method of Step (1) above from 22.5 g of 4-(n-hexyl)amino-3־*nitroquinoline was mixed with 17.1 (0.1152 mole) of triethyl orthoformate and the mixture was heated at 130°C. for 2.5 hours. Evaporation provided a residue which was analyzed by thin layer chromatography on a silica gel plate, eluting with methanol. One spot was detected at Rf=0.8. A small sample of the residue was recrystallized once from diethyl ether to provide solid 1-(n-hexyl)-lH-imidazo[4,5-c]quinoline, m.p. 75-77°C. Analysis: Calculated for ^C16^H19^N3^:%C75.85 ׳: %H, 7.55; %N, 16.6: Found:%C, 75.7; %H, 7.7; %N, 16.7 Step (3)

The crude reaction product from Step (2) above was diluted with 125 ml of glacial acetic acid and 14.0 g (0.1235 mole) of 30% hydrogen peroxide, and the mixture was heated at a bath temperature of 70’C for 22 hours. The glacial acetic acid was removed by adding heptane and by then effecting an azeotropic distillation. The residue was diluted and neutralized with saturated sodium bicarbonate solution. The solid obtained was separated by filtration, washed with water, slurried in diethyl ether, separated by filtration and dried. Recrystallization from ethyl acetate provided 11.8 g of solid l-(n-hexyl)-lH-imidazo[4,5-c]quinolin-5-oxide, m.p. 153-158°C. Step (4)

To a mixture of 6.1 ml (0.0657 mole) of phosphorus oxychloride and 80 ml of Ν,Ν-dimethylformamide was added gradually, with cooling to 10-20°C, 11.8 g (0.0438 mole) of 1-(n-hexyl)-lH-imidazo[1,5-c]quinolin-5-oxide. The solution was allowed to stand at 20°C for 15 minutes, and was then heated on a steam bath for 30 minutes. The solution was cooled and poured over ice with stirring. To the mixture was added concentrated ammonium hydroxide to adjust the pH to 8 to 9. The solid was separated by filtration, washed sequentially with water and diethyl ether, and dried. Recrystallization of a small portion of product from 1:1 ethyl acetate;hexane provided white solid

4-chloro-l-(n-hexyl)-lH-imidazo[4,5-c]quinoline, m.p. 106-108°C. Analysis: Calculated for C₁₆H₁₈C1N3; %C 66.8; %H, 6.3; %N, 14.6; Found %C, 66.8; %H, 6.1: %N, 14.4.

Using the method of Example 1 and/or 2, and starting with the indicated substituted quinolines and primary amines, the following compounds of Formula V were prepared (Table VIII)

TABLE VIII

<td> Ex. No.</td><td> Quinoline Starting Material ____________of Formula IV_______________</td><td> Primary Amine Starting Material</td><td> Intermediate of Formula V (m.p. in.°C)</td>

<td> 176</td><td> 4-chloro-3-nitroquinoline</td><td> 4-chlorobenzylamine</td><td> 4-(4-chlorobenzylamino)3־-nitroquinoline (175-177)</td>

<td> 177</td><td> 4-chloro-3-nitroquinoline</td><td> n־octylamine</td><td> 4-(n-octylamino)-3-nitroquinoline (50-52)</td>

<td> 17b</td><td> 4־׳chloro-3-nitroquinoline</td><td> !-(phenyl)ethylamine</td><td> 4-[l-(phenyl)ethylamino]-3-nitroquinoline (138-141)</td>

<td> 179</td><td> 4-chloro-3-nitroquinoline</td><td> 1,3-dimethylbutylamine</td><td> 4-(l,3-dimethylbutylamino)-3-nitroquinoline (66-68)</td>

<td> 180</td><td> 4״chloro-6,7-dimethoxy-3-nitroquinoline</td><td> isobutylamine</td><td> 6,7-dime thoxy-4-isobutylamino-3-ni tro-</td>

quinoline ,

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Using the method of Example 175/ Step (1)/ 6/7-dimethoxy-4-isobutylamino-3-nitroquinoline was reduced to 3-amino-6/7-dimethoxy-4-isobutylaminoquinoline/ m.p. 159-161’C.

Using the method of Example 175/ Step (1), various intermediates of Formula V were reduced to provide

3-aminoquinolines of Formula VI. These intermediates of Formula VI (usually crude) were cyclized using the method of Example 175, Step (2), to provide the compounds of Formula VII shown in Table IX. .. . _ _ ... W

TABLE IX

<td colspan="5"> Intermediate</td>

<td rowspan="2"> Ex. No.</td><td rowspan="2"> of Formula V (Example)</td><td> Tn tPYrnpd ו a te of Formula VI</td><td> Ortho Eater</td><td> Compounds, of Formula VII __________ (m.p. in *C)________________</td>

<td></td><td></td><td></td>

<td> 182</td><td> 176</td><td> 3-amino-4-(4’-chlorobenzyl־ aminolquinoline</td><td> triethyl orthoacetate</td><td> l-(4-chlorobenzyl)-2-methyl-lHimidazo[4,5-c]quinoline (178-180)</td>

<td> 183</td><td> 175,Step (2)</td><td> 3-amino4־-(n־hexylamino)t quinoline</td><td> triethyl orthoacetate</td><td> 1-(n-hexyl)-2־methyl־lH-imidaz0[4,5-c]qulnoline (88-90)</td>

<td> 184</td><td> 2</td><td> 3-amino4־-(methylamino)quinoline ’</td><td> triethyl orthoisobutyrate</td><td> 2-isobutyM־methyl-lH-imidazo[4,5-c]quinoline (125-127)</td>

<td> 185</td><td> 177</td><td> 3-amino4־-(n-octylamino) quinoline</td><td> triethyl orthoformate</td><td> l-(n-octyl)-lH-imidazo[4|5-c] quinoline (not taken)</td>

<td> 18b</td><td> 1</td><td> lamina-4-(isobutylamino) quinoline</td><td> triethyl orthoisobutyrate</td><td> l_/2־diisobutyl-lH-imidazo[4,5-c]quinoline (93-95)</td>

<td> 187</td><td> 131, Part B</td><td> 3־amino2]־4־’(phenyl)ethylaminolquinoline</td><td> triethyl orthiosobutyrate</td><td> 2-isobutyl-l-[2-(phenyl)ethyl]-lHimidazo[4,5-c]quinoline (92-94)</td>

<td> 188</td><td> 178</td><td> 3-amino4־-[l־(phenyl)ethylaminolquinoline</td><td> triethyl orthoformate</td><td> l-[l-(phenyl)ethyl]-lH-imidazo[4־5_׳c]quinoline (172174־)</td>

<td> 189</td><td> 179</td><td> &gt;amino-4-(l,3־dimethylbutylamino)quinoline</td><td> triethyl orthoformate</td><td> l־(l,3־dimethylbutyl)-lH-imidazo[4/5-dquinoline (83-85)</td>

<td> 198</td><td> 181</td><td> 3־amlno6,7־-dimethoxy־4־ (isobutylamino)quinoline</td><td> triethyl orthoformate (a few drops of formic acid)</td><td> 7,8-dimethoxy-l-isobutyl-lH-imidazo[4/5-clquinoline (163-165)</td>

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Using the method of Example 174, step (3), compounds of Formula VIII shown in Table X were prepared.

TABLE X

Compound of Formula VII (Example No.) _________________Compound of Formula VIII (m.p. in °C) '

162 1-(4-chlorobenzyl)2־-methyl־lH־imidazo[4,5־c]quinolin־5־oxide (251253־) l-(n-butyl)־lH־imidazo[4,5־c]quinolin-5־oxide (161163־) l-(n-hexyl)2״-methyl־lH־imidazo[4,5־c]quinolin-5־oxide (138148־ crude) 2־isobutyl-l-methyl-lH־imidazo[4,5-c]quinolin־5־oxide (202204־)

185 ¹ l־(n־octyl)־lH־imidazo[4,5־c]quinolin־5״oxide (86-90)

186 l,2־_ldiisobutyl־lH־imidazo[4,5־c]quinolin־5־oxide (153156־)

187 2-isobutyl-l2]־-(phenyl)ethyl]־lH־imidazo[4,5־c]quinolin־5־oxide (158-160)

188 l-[l-(phenyl)ethyl]-lH־imidazo[4,5־c]quinolin5־-oxide (not taken), ^a yellow solid, satisfactory elemental analysis ¹

189 l-(l,3־dimethylbutyl)־lH־imidazo[4,5־c]quinolin־5־oxide (not taken), light orange solid

190 7,8-dimethoxy־l־isobutyl־lH־imidazo[4,5־c]quinolin5־-oxide (not taken) co

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Using the method of Example 174, Step (4) , compounds of Formula IX shown in Table XI were prepared.

TABLE XI

Compounds

<td> Ex. of Formula VIII No. (Example No.)</td><td> __Compounds of Formula IX (m.p. in *C) ______________</td>

<td> 201 91 202 191</td><td> 4-chloro-2-methyl-l-[2-(phenyl)ethyl]-lH-imidazo[4,5-c]quinoline (130-140) l-(4-chlorobenzyl)-4-chloro-2-methyl־lH-imidazo[4,5-c]quinoline (240-242)</td>

<td> 203 192 204 193 205 194 206 195 207 196 208 197 209 198</td><td> l-(n-butyl)-4-chloro-lH-imidazo[45_׳-c]quinoline (122-124) 4-chloro-l-(n-hexyl)-2-methyl-lH-imidazo[4,5-c]quinoline (119-121) 4-chloro-2-isobutyl-l-methyl-lH-imidazo[4,5-c]quinoline (158-160) 4-chloro-l־(n-octyl)-lH-imidazo[4,5-c]quinoline (06-90) 4-chloro-l,2-dii30butyl-lH־imidazo[4,5-c]quinoline (137-139) ¹ 4-chloro-2-i3obutyl-l2]־-(phenyl)ethyl]-lH-imidazo[4,5-c]quinoline (151-153) £ 4-chloro-l-[l-(phenyl)ethyl]-lH-imidazo[4,5-c]quinoline (not taken), ! white solid, satisfactory elemental analysis</td>

<td> 210 199 211 200</td><td> 4-chloro-l-(l,3-dimethylbutyl)-lH-imidazo[4,5-c]quinoline (111-114) 4-chloro-7,8-dimethoxy-l-isobutyl-lH-imidazo[4,5-c]quinoline (185-188)</td>

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Example 212

To 70 ml of acetic anhydride was added 13.0 g (0,0539 mole) of l-isobutyl-lH-imidazo[4,5-c]quinolin-5oxide. The solution was heated on a steam bath for 10 minutes, then allowed to cool. The precipitate was separated by filtration, washed with ethanol, and dried. Recrystallization from Ν,Ν-dimethylformamide־ provided

4-hydroxy-l-isobutyl-lH-imidazo[4,5-c]quinoline, m.p. >300°C. Analysis: Calculated for C14H15N3O: %C, 69.7; %H, 6.3; %N, 17.4; Found: %C, 69.8; %H, 6.4; %N, 17.6.

Example 213

To a mixture of 0.5 g (0.0021 mole) of l-isobutyl-lH-imidazo[4,5-c]quinolin-4-amine and 25 ml of 4N-hydrochloric acid was added 2.2 g (0.0315 mole) of sodium nitrite. The mixture was heated on a steam bath for 0.5 hour, and was then allowed to cool. Concentrated ammonium hydroxide was added to adjust the pH of the solution to 8 to 9. The precipitate was separated by filtration, washed with water and dried. Recrystallization from Ν,Ν-dimethylformamide provide white solid l-isobutyl-lH-imidazo[4,5-c]quinolin-4-ol, m.p. >300°C. The identity of the product as that of Example 247 was confirmed by infrared spectral analysis and thin layer chromatography on silica gel, eluting with methanol. Elemental analysis of the product was excellent for the assigned structure.

Example

Step (A)

To 50.0 g (0.269 mole) of 4-hydroxy-3-nitroquinoline in 300 ml of Ν,Ν-dimethylformamide in a 500 ml erlenmeyer flask was added, gradually, 44.3 g (0.2892 mole) of phosphorus oxychloride. The resulting mixture was heated on a steam bath for about 15 minutes, and was then poured onto ice with stirring. After neutralization with saturated sodium bicarbonate solution, the resulting light-colored solid was separated by filtration and washed sequentially with a saturated sodium bicarbonate solution and water. The solid was dissolved in methylene chloride and the solution obtained was dried over sodium chloride, filtered and transferred to a 2 1 erlenmeyer flask. Triethylamine (159.6 g, 1.577 moles) was added at one time, followed by the slow addition of 21.2 g (0.2892 mole) of isobutylamine. After the isobutylamine had been added, the mixture was heated on a steam bath for about 30 minutes. The methylene chloride was removed by rotary evaporation. Water was added to the residue obtained, and concentrated hydrochloric acid was subsequently added to dissolve the residue. The solution was filtered, and the filtrate was brought to pH 8-9 with concentrated ammonium hydroxide.

The resulting yellow solid was filtered, washed with water, and dried to provide 73.4 g of crude 4-isobutylamino-3nitroquinoline, m.p. 114-118°C. The product was further purified by recrystallization from ethanol.

Step (B)

4-isobutylamino-3-nitroquinoline (31.5 g, 0.1284 moles) from Step (A) above, was dissolved in 300 ml of toluene, and 1 g of 5% platinum on carbon was added thereto. The resulting mixture was hydrogenated on a Parr apparatus for one and one-half hours. The mixture was then heated and filtered. Toluene was removed from the filtrate by rotary evaporation to provide 27.8 g of crude

3-amino-4-(isobutylamino)quinoline. Recrystallization twice from ethyl acetate/hexane provided 18.8 g of purified product, m.p. 98-100’C. Analysis: Calculated for ^C13^H17^N3^{: %c}ל⁵.⁷² ׳ %H, ⁸.⁰׳ ^%N5*19 ^׳: Found: %C, 73.2; %H, 7.8; %N, 19.7.

Step (C)

To 10.0 g (0.0464 mole) of 3—amino—4(isobutylamino)quinoline (from Step (B) above) was added 9.0 g (0.0604 mole) of triethyl orthoformate, and the mixture was heated at 125-130’C for three hours. The mixture was then allowed to cool to room temperature, and 30 ml of glacial acetic acid and 7.9 g (0.0696 mole) of 30% hydrogen peroxide solution were added thereto. The resulting mixture was heated at 68-70°C in an oil bath for about 24 hours. The glacial acetic acid was removed by azetropic distillation using heptane as the co-solvent. Saturated sodium bicarbonate solution was added to the residue to bring it to neutrality. The beige solid which precipitated was filtered, washed with water, and dried to provide 10.0 g of crude product 1-isobutyl-lH-imidazo[4,5-c]quinolin-5-oxide. This solid was slurried in a small amount of cold acetone, and was then separated by filtration, washed and dried to provide 6.2 g of purified product having a m.p. of 205-209’C.

Step (D)

To 40 ml of cold Ν,Ν-dimethylformamide (10-20°C) was added slowly 5.9 g (0.0385 mole) of phosphorus oxychloride with swirling, the temperature of the mixture being maintained at 10-20°C. l-isobutyl-lH-imidazo[4,5-c]quinolin-5-oxide (6.2 g: 0.0257 mole) from Step (C) above was added gradually with swirling and cooling. After addition was complete, the solution was allowed to stand at room temperature for about 30 minutes with occasional swirling. The solution was then heated on a steam bath for thirty minutes. After allowing it to cool, the solution was poured onto ice with stirring, and the resulting mixture was brought to pH 8-9 with concentrated ammonium hydroxide. The resulting off-white solid was filtered, washed with water, rinsed with ether, and dried to provide 6.0 g of crude 4-chloro-l-isobutyl-lH-_imidazo[4,5-c]quinoline having a m.p. of 135-138®C.