Antibodies and assays for detection of folate receptor 1

ANTIBODIES AND ASSAYS FOR DETECTION OF FOLATE RECEPTOR 1

FIELD OF THE INVENTION

[0001] The field of this invention generally relates to diagnostic assays and kits for folate receptor 1-based therapies and antibodies that bind to human folate receptor 1.

BACKGROUND OF THE INVENTION

[0002] Cancer is one of the leading causes of death in the developed world, with over one million people diagnosed with cancer and 500,000 deaths per year in the United States alone. Overall it is estimated that more than 1 in 3 people will develop some form of cancer during their lifetime. There are more than 200 different types of cancer, four of which— breast, lung, colorectal, and prostate—account for over half of all new cases (Jemal et al., 2003, Cancer J. Clin. 53:5-26).

[0003] Folate Receptor 1 (FOLRI), also known as Folate Receptor-alpha or Folate Binding Protein, is an N-glycosylated protein expressed on plasma membrane of cells. FOLRI has a high affinity for folic acid and for several reduced folic acid derivatives. FOLRI mediates delivery of the physiological folate, 5-methyltetrahydrofolate, to the interior of cells.

[0004] FOLRI is overexpressed in the vast majority of ovarian cancers, as well as in many uterine, endometrial, pancreatic, renal, lung, and breast cancers, while the expression of FOLRI on normal tissues is restricted to the apical membrane of epithelial cells in the kidney proximal tubules, alveolar pneumocytes of the lung, bladder, testes, choroid plexus, and thyroid (Weitman SD, et al., Cancer Res 52: 3396-3401 (1992); Antony AC, Annu RevNutr 16: 501-521 (1996); Kalli KR, et al. Gynecol Oncol 108: 619-626 (2008)). This expression pattern of FOLR1 makes it a desirable target for FOLRLdirected cancer therapy.

[0005] Because ovarian cancer is typically asymptomatic until advanced stage, it is often diagnosed at a late stage and has poor prognosis when treated with currently available procedures, typically chemotherapeutic drugs after surgical dc-bulking (von Gruenigen V et al. Cancer 112: 2221-2227 (2008); Ayhan A et. al., Am J Obstet Gynecol. 196: 81 e81-86 (2007); Harry ATI et al., Obstet Gynecol Surv 64; 548-560 (2009)). Thus there is a clear unmet medical need for more effective diagnostics for ovarian cancers.

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[0006] Some previous assays used to detect shed FOLRI are not sufficiently specific to FOLRI. For example, some assays do not distinguish between FOLRI and other folate receptor family members (FOLR2, 3, & 4) or report values for total FBP (Folate Binding Protein). Additionally, some assays require that human samples (e.g., plasma) be pre-treated with a light acid wash step to dissociate folic acid from the receptor. Some assay results may also have inaccuracies due to competitive effects between the antibody therapy and diagnostic antibody. Additionally, many commercially available kits are traditionally unreliable both in their reagents, and in their lot-to-lot stability. Evaluations of these kits have given very mixed results, and are intended for research use only. Many require that the human sample be pre-diluted before analysis to reduce the chance of false positives due to the matrix effect. Thus, there is a clear need for highly sensitive and accurate diagnostic assays that can detect a clinically relevant dynamic range of FOLRI as a companion for FOLRIbased therapies.

SUMMARY OF THE INVENTION

[0007] Anti-FOLRl antibodies and antigen-binding fragments thereof as well as methods for detecting FOLRI, diagnosing FOLRI-mediated diseases and disorders (such as cancer), monitoring the efficacy of anti-FOLRl therapies, optimizing anti-FOLRl therapies, and stratifying patients are all provided herein.

[0008] The anti-FOLRl antibodies provided herein can have a diagnostic role. For example, the anti-FOLRl antibodies provided herein to distinguish between tumor and nontumor cells or tissues or to identify tumor types, subtypes, or grades. In one embodiment, an anti-FOLRl antibody provided herein and/or a FOLRI-detection assay provided herein can be used to distinguish between subtypes of non-small cell lung cancer (NSCLC) including adenocarcinoma and squamous cell carcinoma as described herein. In another embodiment, an anti-FOLRl antibody provided herein and/or a. FOLRI-detection assay provided herein can be used to rule out a type of cancer (e.g., to determine that a cell or tissue is not a type of cancer), for example, sarcoma.

[0009] In some embodiments, an antibody or antigen-binding fragment thereof provided herein can specifically bind to an epitope of FOLRI, wherein the epitope comprises at least one, at least two, or three N-glycoslated amino acids. Glycosylation can be critical for membrane localization. See e.g., Yan et al., J. Am. Soc. Nephol. 13: 1385-1389 (2002). Advantageously, the antibodies and antigen-binding fragments herein can detect FOLRI

WO 2015/031815 ־ 3 ־ expression on cell membranes and detect a clinically relevant dynamic range of FOLRI. The more discreet staining obtained with the antibodies and antigen-binding fragments provided herein allows for discrimination among samples all grouped together as high expression levels (with a score of 3) using antibodies that bind to different FOLRI epitopes, lack sufficient specificity, and/or lack sufficient sensitivity.

[0010] In some embodiments, an antibody or antigen-binding fragment thereof provided herein can specifically bind to the same FOLRI epitope as an antibody selected from the group consisting of: (a) an antibody comprising the polypeptide of SEQ ID NO:27 and the polypeptide of SEQ ID NO:28; (b) an antibody comprising the polypeptide of SEQ ID NO:29 and the polypeptide of SEQ ID NO:30; (c) an antibody comprising the polypeptide of SEQ ID NO:31 and the polypeptide of SEQ ID NO:32; (d) an antibody comprising the polypeptide of SEQ ID NO:62 and the polypeptide of SEQ ID NO:63 or SEQ ID NO:64; and (e) an antibody comprising the polypeptide of SEQ ID NO:65 and the polypeptide of SEQ ID NO:66 or SEQ ID NO:67. In some embodiments, the epitope comprises an N-glycosylated amino acid.

[0011] In some embodiments, an antibody or antigen-binding fragment thereof provided herein can specifically bind to FOLRI, wherein said antibody or fragment thereof competitively inhibits binding to FOLRI of an antibody selected from the group consisting of: (a) an antibody comprising the polypeptide of SEQ ID NO ;27 and the polypeptide of SEQ ID NO:28; (b) an antibody comprising the polypeptide of SEQ ID NO:29 and the polypeptide of SEQ ID NO:30; (c) an antibody comprising the polypeptide of SEQ ID NO:31 and the polypeptide of SEQ ID NO:32; (d) an antibody comprising the polypeptide of SEQ ID NO:62 and the polypeptide of SEQ ID NO:63 or SEQ ID NO:64; and (e) an antibody comprising the polypeptide of SEQ ID NO:65 and the polypeptide of SEQ ID NO:66 or SEQ ID NO:67.

[0012] In some embodiments, the antibody or antigen-binding fragment thereof comprises the VH CDRl-3 and VL CDRl-3 polypeptide sequences selected from the group consisting of: (a) SEQ ID NOs:3-8, respectively; (b) SEQ ID NOs;9-14, respectively; (c) SEQ ID NOs:l 5-20, respectively; (d) SEQ ID NOs:21-26, respectively; (c) SEQ ID NOs: 3-5 and SEQ ID NOs: 59, 7, and 8, respectively; (f) SEQ ID NOs: 3, 60, and 5 and SEQ ID NOs: 6-8, respectively; (g) SEQ ID NOs: 3, 61, and 5 and SEQ ID NOs: 6-8, respectively; (h) SEQ ID NOs: 3, 60, and 5 and SEQ ID NOs: 59, 7, and 8, respectively; and (i) SEQ ID NOs: 3, 61, and 5 and SEQ ID NOs: 59, 7, and 8, respectively.

[0013] In some embodiments, an antibody or antigen-binding fragment thereof provided herein can specifically bind to FOLRI, wherein the antibody or fragment thereof

־ - comprises the VH CDRl-3 and VL CDRl-3 polypeptide sequences selected from the group consisting of: (a) SEQ ID NOs:3-8, respectively; (b) SEQ ID NOs:914״, respectively; (c) SEQ ID NOs: 15-20, respectively; (d) SEQ ID NOs:21-26, respectively; (e) SEQ ID NOs: 3-5 and SEQ ID NOs: 59, 7, and 8, respectively; (f) SEQ ID NOs: 3, 60, and 5 and SEQ ID NOs: 6-8, respectively; (g) SEQ ID NOs: 3, 61, and 5 and SEQ ID NOs: 6-8, respectively; (h) SEQ ID NOs: 3, 60, and 5 and SEQ ID NOs: 59, 7, and 8, respectively; (i) SEQ ID NOs: 3, 61, and 5 and SEQ ID NOs: 59, 7, and 8, respectively; and (j) variants of (a) to (i) comprising 1, 2, 3, or 4 conservative amino acid substitutions.

[0014] In some embodiments, the antibody or antigen-binding fragment thereof comprises polypeptide sequences that are at least 90%, at least 95%, or at lesast 99% identical to polypeptide sequences selected from the group consisting of: (a) SEQ ID NO:27 and SEQ ID NO:28; (b) SEQ ID NO:29 and SEQ ID NO:30; (c) SEQ ID NO:31 and SEQ ID NO:32; (d) SEQ ID NO:62 and SEQ ID NO:63 or SEQ ID NO:64; (e) SEQ ID NO:65 and SEQ ID NO:66 or SEQ ID NO:67; (f) SEQ ID NO:68 and SEQ ID NO:69. In some embodiments, the polypeptide sequences comprise, consist essentially of, or consist of the amino acids of sequences selected from the group consisting of: (a) SEQ ID NO:27 and SEQ ID NO:28; (b) SEQ ID NO:29 and SEQ ID NO:30; (c) SEQ ID NO:31 and SEQ ID NO:32; (d) SEQ ID NO:62 and SEQ ID NO:63 or SEQ ID NO:64; (e) SEQ ID NO:65 and SEQ ID NO:66 or SEQ ID NO:67; (Q SEQ ID NO:68 and SEQ ID NO:69.

[0015] In some embodiments, an antibody or antigen-binding fragment thereof provided herein can specifically bind to FOLRI, wherein the antibody or fragment thereof comprises a humanized heavy chain variable region comprising CDRl, CDR2, and CDR3 regions comprising the amino acids of SEQ ID NO:51, SEQ ID NO:52 or 53, and SEQ ID NO:54, respectively, a humanized light chain variable region comprising CDRl, CDR2, and CDR3 regions comprising the amino acids of SEQ ID NO:48, SEQ ID NO:49, and SEQ ID NO:50, respectively, and a murine constant region. In some embodiments, the humanized heavy chain variable region comprises the amino acids of SEQ ID NO:45 and the humanized light chain variable region comprises the amino acids of SEQ ID NO:47.

[00161 In some embodiments, the antibody or antigen-binding fragment thereof is recombinantly produced. In some embodiments, the antibody or antigen-binding fragment thereof is murine, non-human, humanized, chimeric, resurfaced, or human. In some embodiments, the antibody or antigen-binding fragment thereof binds to human FOLRI but not FOLR.2 or FOLR3. In. some embodiments, the antibody or antigen-binding fragment thereof is a full length antibody. In some embodiments, the antibody or antigen-binding

WO 2015/031815 ״ 5 ־ fragment thereof is an antigen-binding fragment. In some embodiments, the antibody or antigen-binding fragment thereof comprises, consists essential! of, or consist of a Fab, Fab', F(ab')2, Fd, single chain Fv or scFv, disulfide linked Fv, V-NAR domain, IgNar, intrabody, IgGACH2, minibody, F(ab')3, tetrabody, triabody, diabody, single-domain antibody, DVDIg, Fcab, mAb2, (scFv)2, or scFv-Fc.

[0017] In some embodiments, a polypeptide provided herein can specifically bind FOLRI, wherein the polypeptide comprises sequences selected from the group consisting of: (a) SEQ ID NOs:3-8, respectively; (b) SEQ ID NOs:9-14, respectively; (c) SEQ ID NOs: 1520, respectively; (d) SEQ ID NOs:21-26, respectively; (e) SEQ ID NOs: 3-5 and SEQ ID NOs: 59, 7, and 8, respectively; (f) SEQ ID NOs: 3, 60, and 5 and SEQ ID NOs: 6-8, respectively; (g) SEQ ID NOs: 3, 61, and 5 and SEQ ID NOs: 6-8, respectively (h) SEQ ID NOs: 3, 60, and 5 and SEQ ID NOs: 59, 7, and 8, respectively; (i) SEQ ID NOs: 3, 61, and 5 and SEQ ID NOs: 59, 7, and 8, respectively; and (j) variants of (a) to (i) comprising 1, 2, 3, or 4 conservative amino acid substitutions. In some embodiments, the polypeptide comprises sequences that are at least 90%, at least 95%, or at least 99% identical to sequences selected from the group consisting of: (a) SEQ ID NO:27 and SEQ ID NO:28; (b) SEQ ID NO:29 and SEQ ID NO130; (c) SEQ ID NO:31 and SEQ ID NO:32; (d) SEQ ID NO:62 and SEQ ID NO:63 or SEQ ID NO:64; (e) SEQ ID NO:65 and SEQ ID NO:66 or SEQ ID NO:67; and (f) SEQ ID NO:68 and SEQ ID NO:69. In some embodiments, the polypeptide comprises the amino acids of (a) SEQ ID NO:27 and SEQ ID NO:28; (b) SEQ ID NO:29 and SEQ ID NO:30; (c) SEQ ID NO:31 and SEQ ID NO:32; (d) SEQ ID NO:62 and SEQ ID NO:63 or SEQ ID NO:64; (e) SEQ ID NO:65 and SEQ ID NO:66 or SEQ ID NO:67; or (f) SEQ ID NO :68 and SEQ ID NO :69.

[0018] In some embodiments, the antibody, antigen-binding fragment thereof, or polypeptide binds to FOLRI with a Kd of about 0.5 to about 10 nM. In some embodiments, the antibody, antigen-binding fragment thereof, or polypeptide binds to a human FOLRI with a Kd of about 1.0 nM or better. In some embodiments, the binding affinity is measured by flow cytometry, Biacorc, ELISA, or radioimmunoassay.

[0019] In some embodiments, the antibody, antigen-binding fragment thereof, or polypeptide binds to an epitope of FOLRI comprising an amino acid that is N-glycosylated.

[0020] In some embodiments, the antibody, antigen-binding fragment thereof, or polypeptide is detectably labeled.

[0021] In some embodiments, a cell provided herein produces the antibody, antigenbinding fragment thereof, or polypeptide. In some embodiments, the cell is isolated.

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[0022] Methods of making the antibody, antigen-binding fragment thereof, or polypeptide are also provided. The methods can comprise (a) culturing a cell provided herein; and (b) isolating the antibody, antigen-binding fragment thereof, or polypeptide from the cultured cell.

[0023] Compositions comprising the antibody, antigen-binding fragment thereof, or polypeptide are also provided. In some embodiments, the composition comprises the the antibody, antigen-binding fragment thereof, or polypeptide and buffer selected from the group consisting of: a FACS buffer, an IHC buffer, and an ELISA buffer.

[0024] Methods of using the antibody, antigen-binding fragment thereof, or polypeptide are also provided.

[0025] In some embodiments, a method of detecting FOLRI expression in a sample comprises contacting the sample with an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein. In some embodiments, the antibody or antigenbinding fragment thereof is detectably labeled. In some embodiments, the label is selected from the group consisting of immunofluorescent label, chemiluminescent label, phosphorescent label, enzyme label, radiolabel, avidin/biotin, colloidal gold particles, colored particles and magnetic particles. In some embodiments, the FOLRI expression is determined by radioimmunoassay, Western blot assay, cytometry, immunofluorescent assay, enzyme immunoassay, immunoprecipitation assay, chemiluminescent assay, or immunohistochemical assay. In some embodiments, the cytometry is flow cytometry. In some embodiments, the FOLRI expression is determined by IHC.

[0026[ In some embodiments, a method for increasing the efficacy of cancer therapy with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof, comprises administering the active agent to a subject having cancer, wherein increased expression of FOLRI has been detected in a cancerous sample from the subject using an antibody, antigen-binding fragment thereof, polypeptide or composition provided herein.

[0027] Tn some embodiments, a method for identifying a cancer likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof comprises: (a) contacting a biological sample comprising cells from the cancer with the antibody, antigen-binding fragment thereof, polypeptide or composition provided herein; (b) detecting binding of the antibody, antibody-fragment, or polypeptide to FOLRI in the biological sample of (a); (c) assigning a score to the binding of step (b), wherein the score is assigned based on comparison to one or more reference samples; and (d) comparing the score

WO 2015/031815 “7 ־ in step (c) to the score of a reference tissue or cell, wherein a score for the cancer FOLRI level that is greater than the score for a normal or low FOLRI expressing reference sample or a score for the cancer FOLRI level that is equal to or greater than the score for a high FOLRI expressing reference sample identifies the cancer as likely to respond to an anti-FOLRl antibody.

[0028] In some embodiments, a method of treating a patient having cancer comprises :(a) determining a FOLRI expression score from a detection of FOLRI expression in a cancerous sample obtained from the patient, wherein the detection is performed using an antibody, antigen-binding fragment thereof, polypeptide or composition provided herein; and (b) administering an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

[0029] In some embodiments, a method of treating a patient having cancer comprises (a) determining a FOLRI expression score from a detection of FOLRI expression in a cancerous sample obtained from the patient, wherein the detection is performed using an antibody, antigen-binding fragment thereof, polypeptide or composition provided herein; and (b) instructing a healthcare provider to administer an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

[0030] In some embodiments, a method of treating a patient having cancer comprises: (a) submitting a cancerous sample taken from a patient having cancer for determining a FOLRI expression score from a detection of FOLRI expression using an antibody, antigenbinding fragment thereof, polypeptide, or composition provided herein; and (b) administering an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

[0031] In some embodiments, a method of treating a patient having cancer comprises:

(a) detecting FOLRI expression in a cancerous sample obtained from the patient, wherein the detection is performed using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; (b) determining a FOLRI expression score for the cancerous sample; and (c) administering an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

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[0032] In some embodiments, a method of treating a patient having cancer comprises: (a) administering to the patient a fixed dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof; (b) detecting the patient's FOLRI relative to the FOLRI level in a reference sample, wherein the detection is performed using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (c) increasing the amount or frequency of subsequent fixed doses if the patient’s FOLRI level is elevated.

[0033] In some embodiments, a method of optimizing a therapeutic regimen with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof for a subject having cancer comprises: (a) administering an increased dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to a subject having cancer wherein an increased expression of FOLRI in the subject has been detected using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; or (b) administering a decreased dose of the active agent to a subject having cancer wherein a decreased expression of FOLRI in the subject has been detected.

[0034] In some embodiments, a method of optimizing a therapeutic regimen with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof for a subject having cancer comprises: (a) detecting the level of FOLRI expression in a cancerous sample from the subject using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; (b) determining a FOLRI expression score for the cancerous sample; and (c) administering an increased dose of an active agent comprising an antiFOLRI antibody or antigen-binding fragment thereof to the subject if the score is low or administering a decreased dose of the active agent to the subject if the score is high.

[0035] In some embodiments, a method of decreasing FOLRI-expressing cancer cells in a cancer patient comprises: (a) detecting the FOLRI level in a cancerous sample taken from a patient, compared to the FOLRI level in a reference sample using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (b) administering to the patient a fixed dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof if the patient’s FOLRI level is elevated compared to the reference sample; wherein the administration of the active agent decreases the number of FOLR!-expressing cancer cells in the patient. In some embodiments, a method of treating cancer in a patient comprises: (a) detecting the FOLRI level in a cancerous sample taken from a patient, compared to the FOLR] level in a reference sample using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided

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WO 2015/031815 ־ 9 ־ herein; and (b) administering to the patient a fixed dose of an active agent comprising an antiFOLRI antibody or antigen-binding fragment thereof if the patient’s FOLRI level is elevated compared to the reference sample; wherein the administration of the active agent decreases the size of a FOLRI-expressing tumor or decreases CA125 levels.

[0036] In some embodiments, a method of decreasing FOLRI-expressing cancer cells in a cancer patient comprises:(a) administering to a patient having a cancer a fixed dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof; (b) detecting the patient's FOLRI level relative to the FOLRI level in a reference sample using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (c) increasing the amount or frequency of subsequent fixed doses if the patient’s FOLRI level is elevated compared to the reference sample; wherein the administration of the active agent decreases the number of FOLRI-expressing cancer cells in. the patient. In some embodiments, a method of treating cancer in a patient comprises; (a) administering to a patient having a cancer a fixed dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof; (b) detecting the patient's FOLRI level relative to the FOLRI level in a reference sample using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (c) increasing the amount or frequency of subsequent fixed doses if the patient’s FOLRI level is elevated compared to the reference sample; wherein the administration of the active agent decreases the size of a FOLRIexpressing tumor or decreases CAI25 levels.

[0037] In some embodiments, a method of monitoring therapeutic efficacy of a fixed dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof in a patient comprises: (a) detecting a first FOLRI level in a biological sample from a patient having, cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; (b) administering to the patient a fixed dose of an active agent comprising an anti-FOLRl antibody or anti gen -binding fragment; (c) detecting a second FOLRI level in a biological sample from the patient following active agent administration, wherein the detecting is performed using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (d) comparing the second FOLRI level to the first FOLRI level; wherein a decrease between the first and second FOLRI level indicates therapeutic efficacy.

[0038| In some embodiments, a method of identifying a subject having a cancer as likely to respond to a low dose anti-FOLRl treatment regimen, comprises; (a) contacting a biological sample comprising cells from the cancer with an antibody, antigen-binding

־ fragment thereof, polypeptide, or composition provided herein; (b) detecting binding of the antibody, antigen-binding fragment, or polypeptide to the biological sample of (a); (c) assigning a score to the binding of step (b), wherein the score is assigned based on comparison, to one or more reference samples; and (d) comparing the score in step (c) to the score of a reference tissue or cell, wherein a score for the cancer FOLRI level that is greater than the score for a normal or low FOLRI expressing reference sample or a score for the cancer FOLRI level that is equal to or greater than the score for a high FOLRI expressing reference sample identifies the cancer as likely to respond to a low dose anti-FOLRl treatment.

[0039] In some embodiments, a method of identifying a cancer as sensitive to treatment with an anti-FOLRl active agent, comprises: (a) detecting the level of FOLRI expression in a cancerous sample from the cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein, wherein the detecting comprises the use of a method that distinguishes between staining intensity or staining uniformity in a FOLRI expressing cancerous sample as compared to staining intensity or staining uniformity in one or more reference samples; (b) determining a FOLRI staining intensity or staining uniformity score for the cancerous sample; and (c) comparing the FOLRI staining intensity or staining uniformity score determined in step (b) to a relative value determined by measuring FOLRI protein expression in at least one reference sample, wherein the at least one reference sample is a tissue, cell, or cell pellet sample which is not sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof and wherein a FOLRI staining intensity score for the cancerous sample determined in step (b) that is higher than the relative value identifies the cancer as being sensitive to treatment with the active agent.

[0040] In some embodiments, a method of identifying a cancer as sensitive to treatment with an anti-FOLRl active agent, comprises: (a) detecting the level of FOLRI expression in a cancerous sample from the cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein, wherein the detecting comprises the use of a method that specifically stains membrane FOLRI in a FOLRI expressing cancerous sample as compared to membrane FOLRI in one or more reference samples; (b) determining a FOLRI score for the cancerous sample; and (c) comparing the FOLRI score determined in step (b) to a relative value determined by measuring FOLRI in at least one reference sample, wherein the at least one reference sample is a tissue, cell, or cell pellet sample which is not sensitive to treatment with an active agent comprising an antiWO 2015/031815 11 ־ -

FOLRI antibody or antigen-binding fragment thereof and wherein a FOLRI score for the cancerous sample determined in step (b) that is higher than the relative value identifies the cancer as being sensitive to treatment with the active agent.

[0041] In some embodiments, a method of identifying a cancer as sensitive to treatment with an anti-FOLRl active agent, comprises: (a) detecting the level of FOLRI expression in a cancerous sample from the cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein, wherein the detecting comprises the use of a method that distinguishes between staining intensity or staining uniformity in a FOLRI expressing cancerous sample as compared to staining intensity or staining uniformity in one or more reference samples; (b) determining a FOLRI staining intensity or staining uniformity score for the cancerous sample; and (c) comparing the FOLRI staining intensity or staining uniformity score determined in step (b) to a relative value determined by measuring FOLRI protein expression in at least one reference sample, wherein the at least one reference sample is a tissue, cell, or cell pellet sample which is sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof and wherein a FOLRI staining intensity score for the cancerous sample determined in step (b) that is greater than or equal to the relative value identifies the cancer as being sensitive to treatment with the active agent.

[0042] In some embodiments, a method of identifying a cancer as sensitive to treatment with an anti-FOLRl active agent, comprises: (a) detecting the level of FOLRI expression in a cancerous sample from the cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein, wherein the detecting comprises the use of a method that specifically stains membrane FOLRI in a FOLRI expressing cancerous sample as compared to membrane FOLRI in one or more reference samples; (b) determining a FOLRI score for the cancerous sample; and (c) comparing the FOLRI score determined in step (b) to a relative value determined by measuring FOLRI in at least one reference sample, wherein the at least one reference sample is a tissue, cell, or cell pellet sample which is sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof and wherein a FOLRI score for the cancerous sample determined in step (b) that is greater than or equal to the relative value identifies the cancer as being sensitive to treatment with the active agent.

[0043] In some embodiments, the method further comprises administering an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to the subject from whom, the cancerous sample or biological sample was obtained.

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[0044] In some embodiments, the patient's FOLRI level is detected in a cancerous sample or biological sample obtained from the patient. In some embodiments, the cancerous sample or biological sample is a bodily fluid, cell, or tissue sample. In some embodiments, the cell is a circulating tumor cell. In some embodiments, the bodily fluid is blood, ascites, urine, plasma, serum, or peripheral blood.

[0045] In some embodiments, the FOLRI is membrane localized FOLRI.

[0046] In some embodiments, the FOLRI is shed FOLRI.

[0047] In some embodiments, the detecting is by enzyme linked immunosorbent assay (ELISA).

[0048] In some embodiments, the detecting is by immunohistochemistry (IHC). In some embodiments, the IHC is calibrated IHC that can distinguish different levels of FOLRI expression. In some embodiments, the IHC produces a range of staining intensity for samples having low cell surface FOLRI expression, intermediate FOLRI cell surface expression, or high FOLRI cell surface expression. In some embodiments, the IHC distinguishes between staining intensity and staining uniformity in a FOLRI expressing cancerous sample or biological sample as compared to a reference sample. In some embodiments, the IHC detects membrane FOLRI. In some embodiments, the IHC is performed manually. In some embodiments, the IHC is performed using an automated system.

[0049] In some embodiments, a FOLRI score is determined from the IHC.

[0050] In some embodiments, a score of at least I indicates an increased expression of FOLR I and identifies the cancer as likely to respond to an active agent comprising an antiFOLRI antibody or antigen-binding fragment thereof.

[0051] In some embodiments, a score of at least 2, at least 2 homo (>75% uniformity), or at least 2 hetero (25-75% uniformity) identifies the cancer as likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer or endometrial cancer. In some embodiments, a score of at least 3, at least 3 homo (>75% uniformity), or at least 3 hetero (25-75% uniformity) identifies the cancer as likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[00521 In some embodiments, an H-scorc of at least 50 identifies a cancer as likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, an H-score of at least 75 identifies an ovarian cancer as likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding

־- fragment thereof. In some embodiments, an H-score of at least 50 identifies an NSCLC as likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, an H-score of at least 50 identifies an endometrial cancer as likely to respond to an active agent comprising na anti-FOLRl antibody or antigenbinding fragment thereof. In one embodiment, an H-score is determined using the FOLRI2.1 antibody.

[0053] In some embodiments, at least 25% of FOLRI membrane expression in an ovarian tumor sample with an intensity of at least 3 identifies the ovarian cancer as likely to respond to an active agent comprising an. anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, at least 25% of FOLRI membrane expression in an NSCLC sample with an intensity of at least 2 identifies the NSCLC as likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, at least 25% of FOLRI membrane expression in an endometrial tumor sample with an intensity of at least 2 identifies the endometrial cancer as likely to respond to an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In one embodiment, the expression score is determined using the FOLRI -2.1 antibody.

[0054] In some embodiments, a score of at least 1 indicates an increased expression of FOLRI and that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, a score of at least 2, at least 2 homo (>75% uniformity), or at least 2 hetero (25-75% uniformity) indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer or endometrial cancer. In some embodiments, a score of at least 3, at least 3 homo (>75% uniformity), or at least 3 hetero (25-75% uniformity) indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[0055] In some embodiments, an H-scorc of at least 50 indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, an. H-scorc of at least 75 indicates that a patient with ovarian cancer will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, an H-score of at least 50 indicates that a patient with NSCLC will benefit from administration of an active agent comprising an anti-FOLR l antibody or antigen-binding

WO 2015/031815 ־ 14 ־ fragment thereof. In some embodiments, an H-score of at least 50 indicates that a patient with endometrial cancer will benefit from administration of an active agent comprising an anti-FOLK I antibody or antigen-binding fragment thereof. In one embodiment, an H-score is determined using the FOLRI-2.1 antibody.

[0056] In some embodiments, at least 25% of FOLRI membrane expression in a ovarian tumor sample with an intensity of at least 3 indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or anti gen-binding fragment thereof. Tn some embodiments, at least 25% of FOLRI membrane expression in an NSCLC sample with an intensity of at least 2 indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, at least 25% of FOLR I membrane expression in an endometrial tumor sample with an intensity of at least 2 indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof. In one embodiment, the expression score is deteremined using the FOLRI-2.1 antibody.

[0057] In some embodiments, a score of at least 1 indicates an increased expression of FOLRI. In some embodiments, a score of at least 2, at least 2 homo (>75% uniformity), or at least 2 hetero (25-75% uniformity) indicates a decreased dose of the active agent should be administered. In some embodiments, the cancer is lung cancer or endometrial cancer. In some embodiments, a score of at least 3, at least 3 homo (>75% uniformity), or at least 3 hetero (25-75% uniformity) indicates a decreased dose of the active agent should be administered. Tn some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[0058[ In some embodiments, a score of at least 1 indicates an increased expression of FOLRI. In some embodiments, a score of at least 2, at !east 2 homo (>75% uniformity), or at least 2 hetero (25-75% uniformity) identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. Tn some embodiments, the cancer is lung cancer or endometrial cancer. In some embodiments, a score of at least 3, at least 3 homo (>75% uniformity), or at least 3 hetero (25 75% uniformity) identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. In some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[0059] In some embodiments, a score of at !cast 2, at least 2 homo (>75% uniformity), or at least 2 hetero (25-75% uniformity) identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding

WO 2015/031815 ־ 15 ־ fragment thereof. In some embodiments, the cancer is lung cancer or endometrial cancer. In some embodiments, a score of at least 3, at least 3 homo (>75% uniformity), or at least 3 hetero (25-75% uniformity) identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[0060] In some embodiments, an H-score of at least 50 identifies a cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen״ binding fragment thereof. In some embodiments, an H-score of at least 75 identifies an ovarian cancer as being sensitive to treatment with an active agent comprising an anti-FOLR l antibody or antigen-binding fragment thereof. In some embodiments, an H-score of at least 50 identifies an NSCLC as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or anti gen-binding fragment thereof. In some embodiments, an Hscore of at least 50 identifies an endometrial cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In one embodiment, an H-score is determined using the FOLRI-2.1 antibody.

[0061] In some embodiments, at least 25% of FOLRI membrane expression in a ovarian tumor sample with an intensity of at least 3 identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, at least 25% of FOLRI membrane expression in an NSCLC sample with an intensity of at least 2 identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, at least 25% of FOLRI membrane expression in an endometrial tumor sample with an intensity of at least 2 identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof. In one embodiment, the expression score is deteremined using the FOLR 1-2.1 antibody.

[0062] In some embodiments, the reference sample is a positive reference sample or a negative reference sample. In some embodiments, the reference sample comprises cells, cell pellets, or tissue.

[0063] In sonic embodiments, the antibody, antigen-binding fragment thereof, or polypeptide of comprises a detection reagent selected from the group consisting of: an enzyme, a fluorophore, a radioactive label, and a luminophore. In some embodiments, the detection reagent is selected from the group consisting of: biotin, digoxigenin, fluorescein, tritium, and rhodamine.

WO 2015/031815 “16־

[0064] In some embodiments, the cancer is a FOLRI positive cancer. In some embodiments, the cancer is selected from the group consisting of ovarian, brain, breast, uterine, endometrial, pancreatic, renal, and lung cancer. In some embodiments, the lung cancer is non small cell lung cancer or bronchioloalveolar carcinoma. In some embodiments, the ovarian cancer is epithelial ovarian cancer. In some embodiments, the ovarian cancer is platinum resistant, relapsed, or refractory.

[0065] In some embodiments, FOLRI expression is detected using at least one additional anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, FOLRI expression is measured using two anti-FOLRl antibodies or antigen-binding fragments thereof. In some embodiments, at least one antibody or antigen-binding fragment thereof is bound to a solid support. In some embodiments, at least one antibody or antigenbinding fragment thereof is bound to a microtiter plate.

[0066] In some embodiments, at least one additional antibody or antigen-binding fragment thereof comprises a detection agent. In some embodiments, the detection agent is a chromogenic detection agent, a fluorogenic detection agent, an enzymatic detection agent, or an electrochemiluminescent detection agent. In some embodiments, the detection agent is horseradish peroxidase (HRP).

[0067] In some embodiments, the ELISA is a sandwich ELISA.

[0068] In some embodiments, the active agent comprises the FOLRI antibody huM0v!9. In some embodiments, the active agent is an antibody maytansinoid conjugate comprising the FOLRI antibody huMovl9 (comprising a heavy chain variable region of SEQ ID NO:45 and a light chain variable region of SEQ ID NO.47־), the maytansinoid DM4, and the cleavable sulfo-SPDB linker (IMGN853).

[0069] In some embodiments, a method for identifying a cancer as likely to respond to treatment with an. antibody maytansinoid conjugate comprising the FOLRI antibody huMov’19, the maytansinoid DM4 and a sulfo-SPDB linker (1MGN853), comprises measuring FOLRI using an antibody comprising a heavy chain comprising the amino acids of SEQ ID NO:27 and a light chain comprising the amino acids of SEQ ID NO:28 in an IHC assay, wherein a score of at least 2 hetero indicates the cancer is likely to responds to the treatment.

[0070] In some embodiments, a method for identifying a cancer as likely to respond to treatment with an antibody maytansinoid conjugate comprising the FOLRI antibody huMovl9, the maytansinoid DM4 and a sulfo-SPDB linker (IMGN853), comprises measuring FOLRI using an antibody comprising a heavy chain comprising the amino acids

WO 2015/031815 ״ 17 ־ of SEQ ID NO:27 and a light chain comprising the amino acids of SEQ ID NO:28 in an IHC assay, wherein a. score of at least 1 indicates the cancer is likely to responds to the treatment.

[0071] In some embodiments, an article of manufacture provided herein comprises a therapeutic active agent comprising an anti-FOLR1 antibody or antigen-binding fragment thereof described herein, a container, and a package insert or label indicating that the active agent can be used to treat a cancer characterized by the increased expression of FOLRI. In some embodiments, an article of manufacture provided herein comprises a therapeutic active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof described herein, a container, and a package insert or label indicating that the active agent can be used to treat, a cancer characterized by the expression of FOLRI at a level of 2, or 3 measured using, an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein. In some embodiments, the anti-FOLRl antibody of the active agent is conjugated to a cytotoxin. In some embodiments, the package insert or label indicates that the active agent can be used to treat a cancer characterized by the expression of FOLRI at a level of at least 1. In some embodiments, the package insert or label indicates that the active agent can be used to treat a cancer characterized by the expression of FOLRI at a level of at least 2, at least 2 homo (>75% uniformity), or at least 2 hetero (25-75% uniformity) In some embodiments, the cancer is lung cancer or endometrial cancer. In some embodiments, the package insert or label indicates that the active agent can be used to treat a cancer characterized by the expression of FOLRI at a level of at least 3, at least 3 homo (>75% uniformity), or at least 3 hetero (25-75% uniformity). In some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[0072] . In some embodiments, a combination diagnostic and pharmaceutical kit provided herein comprises an antibody, anti gen-binding fragment thereof, polypeptide, or composition provided herein for use in diagnosis and an active agent comprising an antiFOLRI antibody or antigen-binding fragment thereof for use in therapy. In some embodiments, the detection antibody is able to detect FOLRI expression by IHC. In some embodiments, the detection antibody is able to detect FOLRI expression by ELISA. In some embodiments, the anti-FOLRl antibody in the active agent is conjugated to a cytotoxin.

[00731 In some embodiments, a diagnostic kit provided herein comprises an antibody, antigen-binding fragment thereof or polypeptide provided herein, a reagent for immunohistochemistry (IHC), and one or more standardized reference samples, wherein the standardized reference samples comprise cells, cell pellets, or formalin fixed paraffin embedded tissue samples, and wherein the one or more standardized referenced samples arc

WO 2015/031815 ־ 18 ־ from non-FOLRl expressing, low-FOLRl expressing, or high FOLRI expressing cells, cell pellets, or tissues.

[0074] In some embodiments, an immunoassay kit for detecting shed FOLRI in a sample comprises: (a) an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein, and (b) a detection reagent. In some embodiments, the kit further comprises a solid support for the capture reagent. In some embodiments, the capture reagent is immobilized on the solid support. In some embodiments, the capture reagent is coated on a microtiter plate. In some embodiments, the detection reagent is a second FOLRI antibody. In some embodiments, the detection reagent is detected using a species specific antibody. In some embodiments, the kit further comprises a detection means for the detection reagent. In some embodiments, the detection means is colorimetric. In some embodiments, the kit further comprises a FOLRI polypeptide as an antigen standard. In some embodiments, the FOLRI polypeptide is FOLRl-Fc.

[0075] Active agents are also provided herein. In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, wherein said active agent is administered to a subject having cancer, wherein increased expression of FOLRI has been detected in a cancerous sample obtained from said subject using an antibody, antigen-binding fragment thereof, polypeptide or composition provided herein.

[0076] In some embodiments, an active agent comprises an anti-FOLRI antibody or antigen-binding fragment thereof for use in a method for treating cancer, comprising: (a) determining a FOLRI expression score from a detection of FOLRI expression in a cancerous sample obtained from the patient, wherein the detection is performed using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (b) administering an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

[0077] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, comprising: (a) determining a FOLRI expression score from a detection of FOLRI expression in a cancerous sample obtained from the patient, wherein the detection is performed using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (b) instructing a healthcare provider to administer an active agent comprising an anti-FOLRl

WO 2015/031815 ־19־ antibody or antigen-binding fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

[0078] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, comprising: (a) submitting a cancerous sample obtained from a patient having cancer for determining a FOLRI expression score from a detection of FOLRI expression using the antibody, antigenbinding fragment thereof, polypeptide, or composition provided herein; and (b) administering an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

[0079] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, comprising: (a) detecting FOLRI expression in a cancerous sample obtained from said patient,wherein the detection is performed using the antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; (b) determining a FOLRI expression score for said cancerous sample; and (c) administering an active agent comprising an anti-FOLRl antibody or antigenbidning fragment thereof to the patient if the score indicates the patient will benefit from administration of the active agent.

[0080] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, comprising: (a) administering to a patient a fixed dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof; (b) detecting the FOLRI expression level in a cancerous sample obtained from the patient relative to the FOLRI level in a reference sample,wherein the detection is performed using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (c) increasing the amount or frequency of subsequent fixed doses if the patient’s FOLRI level is elevated.

[0081] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, comprising the step of optimizing the therapeutic regimen of said active agent comprising: (a) administering an increased dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to a subject having cancer wherein an increased expression of FOLRI in a cancerours sample from said subject has been detected using the antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; or (b) administering a

I

I

־ - decreased dose of the active agent to a subject having cancer wherein a decreased expression of FOLRI in a cancerous sample from said subject has been detected.

[0082] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, comprising the step of optimizing the therapeutic regimen of said active agent comprising: (a) detecting the level of FOLRI expression in a cancerous sample from said subject using an antibody, antigenbinding fragment thereof, polypeptide, or composition provided herein; (b) determining a FOLRI expression score for said cancerous sample; and (c) administering an increased dose of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof to the subject if the score is low or administering a decreased dose of the active agent to the subject if the score is high.

[0083] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, wherein FOLRIexpressing cancer cells in a cancer patient are decreased, wherein: (a) the FOLRI level in a cancerous sample obtained from a patient is detected by comparing it to the FOLRI level in a reference sample using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (b) a fixed dose of the active agent is administered to the patient if the patient’s FOLRI level is elevated; wherein the administration of the active agent decreases the number of FOLRI-expressing cancer cells in the patient.

[0084] In some embodiments, an active agent comprises an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for treating cancer, wherein FOLRIexpressing cancer cells in a cancer patient arc decreased, wherein: (a) a fixed dose of the active agent is administered to a patient having a cancer; (b) the FOLRI level in a cancerous sample obtained from the patient is detected relative to the FOLRI level in a reference sample using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (c) the amount or frequency of subsequent fixed doses is increased if the patient’s FOLRI level is elevated compared to the reference sample; wherein the administration of the active agent decreases the number of FOLRl-cxpressing cancer cells in the patient.

[0085] Anti-FOLRl antibodies and antigen-binding fragments thereof for uses is methods of monitoring and methods of diagnosing are also provided herein. Tn some embodiments, an anti-FOLRl antibody or antigen-binding fragment thereof tor use in a method for monitoring the therapeutic efficacy of a fixed dose of the active agent in a patient comprises: (a) detecting a first FOLRI level in a biological sample from a patient having

WO 2015/031815 ־21־ cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; (b) administering to the patient a fixed dose of the active agent; (c) detecting a second FOLRI level in a biological sample from the patient following active agent administration, wherein the detecting is performed using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; and (d) comparing the second FOLRI level to the first FOLRI level; wherein a decrease between the first and second FOLRI level indicates therapeutic efficacy.

[0086] In some embodiments, an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for diagnosing whether a subject having cancer is likely to respond to a low dose anti-FOLRl treatment regimen, comprises: (a) contacting a biological sample comprising cells from said cancer with an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein; (b) detecting binding of said antibody, antigenbinding fragment, or polypeptide to said biological sample of (a); (c) assigning a score to said binding of step (b), wherein said score is assigned based on comparison to one or more reference samples; and (d) comparing said score in step (c) to the score of a reference tissue or cell, wherein a score for said cancer FOLRI level that is greater than the score for a normal or low FOLRI expressing reference sample or a score for said cancer FOLRI level that is equal to or greater than the score for a high FOLRI expressing reference sample identifies said cancer as likely to respond to a low dose of an active agent comprising an antiFOLRI antibody or antigen-binding fragment thereof.

[0087] In some embodiments, an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for diagnosing whether a cancer is sensitive to treatment with an. anti-FOLRl treatment, comprises: (a) detecting the level of FOLRI expression in a cancerous sample from said cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein, wherein said detecting comprises the use of a method that distinguishes between staining intensity or staining uniformity in a FOLR1 expressing cancerous sample as compared to staining intensity or staining uniformity in one or more reference samples; (b) determining a FOLRI staining intensity or staining uniformity score for said cancerous sample; and (c) comparing the FOLRI staining intensity or staining uniformity score determined in step (b) to a relative value determined by measuring FOLRI protein expression in at least one reference sample, wherein said at least one reference sample is a tissue, cell, or cell pellet sample which is not sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof and wherein a FOLR1 staining intensity score for said cancerous sample determined in step (b) that is

־ - higher than said relative value identifies said cancer as being sensitive to treatment with the active agent.

[0088] In some embodiments, an anti-FOLRl antibody or antigen-binding fragment thereof for use in a method for diagnosing whether a cancer is sensitive to treatment with an anti-FOLRl treatment, comprising: (a) detecting the level of FOLRI expression in a cancerous sample from said cancer using an antibody, antigen-binding fragment thereof, polypeptide, or composition provided herein, wherein said detecting comprises the use of a method that distinguishes between staining intensity or staining uniformity in a FOLRI expressing cancerous sample as compared to staining intensity or staining uniformity in one or more reference samples; (b) determining a FOLRI staining intensity or staining uniformity score for said cancerous sample; and (c) comparing the FOLRI staining intensity or staining uniformity score determined in step (b) to a relative value determined by measuring FOLRI protein expression in at least one reference sample, wherein said at least one reference sample is a tissue, cell, or cell pellet sample which is sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof and wherein a FOLRI staining intensity score for said cancerous sample determined in step (b) that is higher than said relative value identifies said cancer as being sensitive to treatment with the active agent.

[0089] In some embodiments, the use of the active agents or anti-FOLRl antibodies or antigen-binding fragments thereof further comprises administering an active agent comprising an anti-FOLRl antibody or antigen-fragment thereof to the subject from whom the cancerous sample or biological sample was obtained.

[0090] Tn some embodiments, the cancerous sample or biological sample is a bodily fluid, cell, or tissue sample. In some embodiments, the cell is a circulating tumor cell. In some embodiments, the bodily fluid is blood, ascites, urine, plasma, serum, or peripheral blood.

[0091] Tn some embodiments of the active agents or anti-FOLRl antibodies or antigen-binding fragmetns thereof, the detecting is by enzyme linked immunosorbent assay (ELISA) and/or by immunohistochemistry (IHC). In some embodiments, the IBC is calibrated IHC that can distinguish different levels of FOLRI expression. In some embodiments, the IHC produces a range of staining intensity for samples having low cell surface FOLR I expression, intermediate FOLR1 cell surface expression, or high FOLRI cell surface expression. In some embodiments, the IHC distinguishes between staining intensity and staining uniformity in a FOLRI expressing cancerous sample or biological sample as

־ - compared to a reference sample. In some embodiments, IHC is performed manually. In some embodiments, the IHC is performed using an automated system. In some embodiments, a FOLRI score is determined from the IHC. In some embodiments, the IHC with an antibody 01־ antigen-binding fragment described herein produces a range of staining for cells that have increased FOLRI expression, particularly those within the level of staining equal to or greater than 2.

[0092] In some embodiments, a score of at least 2 indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. Tn some embodiments, a score of at least 2 homo (>75% uniformity) or at least 2 hetero (25-75% uniformity) indicates that the patient will benefit from administration of an active a'gent comprising an anti-FOLRl antibody or antigenbinding fragment thereof. In some embodiments, the cancer is lung cancer or endometrial cancer.

[0093] In some embodiments, a score of at least 3 indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof In some embodiments, a score of at least 3 homo (>75% uniformity) or at least 3 hetero (25-75% uniformity) indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof. In some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[0094] Tn some embodiments, a score of at least 2 indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof In some embodiments, a score of at least 2 homo (>75% uniformity) or at least 2 hetero (25-75% uniformity) indicates that the patient will benefit from administration of an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof. In some embodiments, the cancer is lung cancer, endometrial cancer, or ovarian cancer.

[0095] In some embodiments, a score of at least 2 indicates an a decreased dose of the active agent should be administered. Tn some embodiments, a score of at least 2 homo (>75% uniformity) or at least 2 hetero (25-75% uniformity) indicates an a decreased dose of the active agent should be administered. In some embodiments, the cancer is lung cancer, endometrial cance, or ovarian cancer.

[0096] Tn some embodiments, a score of at least 2 identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. In some embodiments, a score of at least 2

WO 2015/031815 ־ 24־ homo (>75% uniformity) or 2 hetero (2575%־ uniformity) identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. In some embodiments, the cancer is lung cancer or endometrial cancer.

[0097] In some embodiments, a score of at least 3 identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. In some embodiments, a score of at least 3 homo (>75% uniformity) or at least 3 hetero (25-75% uniformity) identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. In some embodiments, the cancer is lung cancer, endometrial cance, or ovarian cancer.

[0098[ In some embodiments, a score of at least 2 identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. In some embodiments, a score of at least 2 homo (>75% uniformity) or at least 2 hetero (25-75% uniformity) identifies the cancer as likely to respond to a low dose anti-FOLRl treatment. In some embodiments, the cancer is lung cancer, endometrial cance, or ovarian cancer.

[0099] In some embodiments, a score of at least 2 identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof. In some embodiments, a score of at least 2 homo (>75% uniformity) or at least 2 hetero (25-75% uniformity) identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer or endometrial cancer.

[0100] In some embodiments, a score of at least 3 identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof. In some embodiments, a score of at least 3 homo (>75% uniformity) or at least 3 hetero (25-75% uniformity) identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer, endometrial cance, or ovarian cancer.

[0101] In some embodiments, a score of at least 2 identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigenbinding fragment thereof, in some embodiments, a score of at least 2 homo (>75% uniformity) or at least 2 hetero (25-75% uniformity) identifies the cancer as being sensitive to treatment with an active agent comprising an anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the cancer is lung cancer, endometrial cance, or ovarian cancer.

to^x—ד׳------—.: μ״״״״1׳—ל״™-——״־י־י־ ^ζχΑ׳.'.i :< ζ<γ ׳ .-

WO 2015/031815 “ 25 -

[0102] In some embodiments, a reference sample is a positive reference sample or a negative reference sample. In some embodiments, the reference sample comprises cells, cell pellets, or tissue.

[0103] In some embodiments of the active agent or anti-FOLRl antibody or antigenbinding fragment thereof for a use provided herein, the antibody, antigen-binding fragment, or polypeptide provided herein further comprises a detection reagent selected from the group consisting of: an enzyme, a fluorophore, a radioactive label., and a luminophore. In some embodiments, the detection reagent is selected from the group consisting of: biotin, digoxigenin, fluorescein, tritium, and rhodamine.

[0104] In some embodiments of the active agent or anti-FOLRl antibody or antigenbinding fragment thereof for a use provided herein, the cancer is a FOLRI positive cancer. In some embodiments, the cancer is selected from the group consisting of ovarian, brain, breast, uterine, endometrial, pancreatic, renal, and lung cancer. In some embodiments, the lung cancer is non small cell lung cancer or bronchioloalveolar carcinoma. In some embodiments, the ovarian cancer is epithelial ovarian cancer. In some embodiments, the ovarian cancer is platinum resistant, relapsed, or refractory.

[0105] In some embodiments of the active agent or anti-FOLRl antibody or antigenbinding fragment thereof for a use provided herein, the FOLRI expression is detected using at least one additional anti-FOLRl antibody or antigen-binding fragment thereof. In some embodiments, the FOLRI expression is measured using two anti-FOLRl antibodies or antigen-binding fragments thereof. In some embodiments, at least one antibody or antigenbinding fragment thereof is bound to a solid support. In some embodiments, at least one antibody or antigen-binding fragment thereof is bound to a microtiter plate. In some embodiments, at least one antibody or antigen-binding fragment thereof comprises a detection agent. In some embodiments, the detection agent is a chromogenic detection agent, a fluorogenic detection agent, an enzymatic detection agent, or an electrochemiluminescent detection agent. In some embodiments, the detection agent is horseradish peroxidase (HRP), In some embodiments, the ELISA is a sandwich ELISA.

[0106] In some embodiments of the active agent or anti-FOLRl antibody or antigenbinding fragment thereof for a use provided herein, the active agent comprises the FOLRI antibody huMovl9 or is the FOLRI antibody huMovl9. In some embodiments, the active agent is administered as an antibody maytansinoid conjugate further comprising the maytansinoid DM.4 and the cleavable sulfo-SPDB linker (JMGN853).

־ '

[0107] In some embodiments, an antibody, antigen-binding fragment, polypeptide, or composition provided herein is for use as a diagnostic.

[0108] In some embodiments, an antibody, antigen-binding fragment, polypeptide, or composition provided herein is for use in a method for diagnosing cancer in a patient suffering therefrom. In some embodiments, the cancer is associated with elevated levels of FOLRI.

[0109] In some embodiments, the binding affinity of an antibody, antigen-binding fragment, or polypeptide is a binding affinity obtained in Example 3 and/or shown in Figure 4, 5, and/or 6.

BRIEF DESCRIPTIONS OF THE DRAWINGS

[0110] Figure 1 provides images of IHC staining of NSCLC and ovarian endometrioid adenocarcinoma samples using the 353.2-1 and 353.9-20 antibodies.

[0111] Figure 2 provides images of IHC staining of normal salivary gland and pancreas samples using the 353.2-1 and 353.9-20 antibodies.

[0112] Figure 3 provides images of Western blots of cell lysates using the 353.9-21, 353.2-1, 353.3-8, and 353.5-7 antibodies.

[0113] Figure 4 shows the binding of 353.2-1, 353.3-1, 353.5-7, and 353.9-21 antibodies to denatured KB cells (A) and non-denatured T47D cells (B) using a fluorescence activated cell sorter (FACS).

[0114] Figure 5 shows the binding of 353.2-1, 353.3-1, 353.5-7, and 353.9-21 antibodies to recombinant human FOLRI using ELISA.

[0115] Figure 6 shows the binding of an anti-FOLR2 antibody and 353.2-1, 353.3-1, 353.5-7, and 353.9-21 antibodies to FOLR2 (A) and the binding of an anti-FOLR3 antibody and 353.2-1, 353.3-1, 353.5-7, and 353.9-21 antibodies to FOLR3 (B) by ELISA.

[0116] Figure 7 shows the binding of anti-FOLRl antibodies 2.1 and huMovl9 to deglycosylated and non-treated recombinant human FOLRI by ELISA.

[0117] Figure 8 shows the binding of anti-FOLRl antibodies 2.1, huM0vl9, and. BN3.2 to deglysolyatcd and non-treated lysates of KB and Igrov-1 cells by western blot analysis.

[0118| Figure 9 shows the relevant amino acids for resurfacing of the anti-FOLRl FRIHC2-1 antibody and the kabat position corresponding to each residue.

־ -

[0119] Figure 10 shows the alignment of murine and humanized FRIHC2-1 antibody sequences for resurfacing. The murine heavy and light chain sequences correspond to SEQ ID NO:27 and SEQ ID NO:28, respectively. The resurfaced humanized heavy chain sequence corresponds to SEQ ID NO: 62, and the resurfaced human light chain version 1.0 and version 1.1 sequences correspond to SEQ ID NO;63 and SEQ ID NO:64, respectively. The leader S in the light chain sequence (framework position -1) is not considered for humanization and is not used in the humanized antibody sequence, so it is not shown in the figure.

[0120[ Figure 11 shows the relevant amino acids for CDR grafting of the anti-FOLRl FRIHC2-1 antibody and the kabat position corresponding to each residue.

[0121] Figure 12 shows the alignment of murine and. humanized FR1HC2-1 sequences for CDR grafting. The murine heavy and light chain sequences correspond to SEQ ID NO:27 and SEQ ID NO:28, respectively. The grafted humanized heavy chain sequence corresponds to SEQ ID NO; 65, and the grafted human light chain version 1.0 and version 1.1 sequences correspond to SEQ ID NO:66 and SEQ ID NO:67, respectively. The leader S in the light chain sequence (framework position -1) is not considered for humanization and is not used in the humanized antibody sequence, so it is not shown in the figure.

[0122] Figure 13 provides images of IHC staining of lung adenocarcinoma tissues using the FOLRI-2.1 (353-2.1) antibody at varying dilutions.

[0123] Figure 14 provides images of IHC staining of positive normal tissue (fallopian tube) (A) and cells (FOLRI transfected cells (B)) and negative cells (untransfected cells (C)) using the FOLRI-2.1 (353-2.1) antibody.

[0124] Figure 15 provides images of IHC staining of ovarian cancer tissue (A) and lung adenocarcinoma tissue (B) samples using the FOLRI-2.1 (353-2.1) antibody.

[0125] Figure 16 shows membrane staining of tumor cells in an endometrial cancer sample with the FOLRI-2.1 assay. The stromal cells arc not stained.

[0126] Figure 17 shows a comparison of staining and scoring difference between (A) the FOLRI-2.1 assay and (B) the BN3.2 assay.

DETAILED DESCRIPTION OF THE INVENTION

[0127] The present disclosure provides methods of detecting human folate receptor 1 (FOLRI), including membrane FOLRI, shed FOLRI, and FOLRI on circulating tumor cells, and improving the efficacy of or the likelihood of response to the treatment of cancers

־־ - characterized by the overexpression of FOLRI, The detection methods can detect a clinically relevant dynamic range of FOLRI and therefore can be used for patient stratification, to monitor or determine therapeutic efficacy, or the likelihood of response to the treatment of cancers characterized by the over expression of FOLRL Novel FOLRI-binding polypeptides, such as antibodies, that are useful in the FOLRI detection methods (e.g., IHC for membrane bound and cell associated FOLRI) are also disclosed. Related polypeptides and polynucleotides, compositions comprising the FOLRI-binding agents, and methods of making the FOLRI-binding agents arc also provided.

I. Definitions

[0128] To facilitate an understanding of the present invention, a number of terms and phrases arc defined below.

[0129] The terms human folate receptor 1, FOLRI, or folate receptor alpha (FRa), as used herein, refers to any native human FOLRI, unless otherwise indicated. Thus, all of these terms can refer to either a protein or nucleic acid sequence as indicated herein. The term FOLRI encompasses full-length, unprocessed FOLRI as well as any form of FOLRI that results from processing within the cell. The term also encompasses naturally occurring variants of FOLRI protein or nucleic acid, e.g., splice variants, allelic variants and isoforms. The FOLRI polypeptides and polynucleotides described herein can be isolated from a variety of sources, such as from human tissue types or from another source, or prepared by recombinant or synthetic methods. Examples of FOLRI sequences include, but are not limited to NCBI reference numbers P15328, NP_001092242.1, AAX29268.1, AAX37119.1, NP_057937.1, and NP_057936.1.

[0130] The terms shed antigen and shed FOLR1 are used interchangeably herein. These terms refer to a FOLRI protein that is soluble and that is not cell associated. In some embodiments it includes the extracellular domain (ECD) and the glycosylphosphatidyl inositol (GPI) linker. In one embodiment, the shed FOLRI includes only the ECD. FOLRI protein includes a signal peptide (amino acids 1-24), the FOLRI protein chain (amino acids 25-233 or 234), and a propeptide which can be cleaved (amino acids 235 to 257). Mature FOLRI protein lacks the signal peptide. Shed FOLRI can include amino acids 1 to 257, 1 to 233, 1 to 234, 25 to 233, 25 to 234. or any other fragments thereof. In some embodiments the signal sequence is cleaved. In other embodiments the ECD and the GPI portion can be embedded in a membrane (e.g., a soluble lipid raft). In one embodiment, the shed FOLRI can include amino acids 1-233 or a fragment thereof.

־ -

[0131] The term antibody means an immunoglobulin molecule that recognizes and specifically binds to a target, such as a protein, polypeptide, peptide, carbohydrate, polynucleotide, lipid, or combinations of the foregoing through at least one antigen recognition site within the variable region of the immunoglobulin molecule. As used herein, the term antibody encompasses intact polyclonal antibodies, intact monoclonal antibodies, antibody fragments (such as Fab, Fab', F(ab')2, and Fv fragments), single chain Fv (scFv) mutants, multispecific antibodies such as bispecific antibodies, chimeric antibodies, humanized antibodies, human antibodies, fusion proteins comprising an antigen determination portion of an antibody, and any other modified immunoglobulin molecule comprising an antigen recognition site so long as the antibodies exhibit the desired biological activity. An antibody can be of any of the five major classes of immunoglobulins: IgA, IgD, IgE, IgG, andlgM, or subclasses (isotypes) thereof (e.g., IgGl, IgG2, IgG3, IgG4, IgAl and IgA2), based on the identity of their heavy-chain constant domains referred to as alpha, delta, epsilon, gamma, and mu, respectively. The different classes of immunoglobulins have different and well known subunit structures and three-dimensional configurations. Antibodies can be naked or conjugated to other molecules such as toxins, radioisotopes, etc.

[0132] In some embodiments, an antibody is a non-naturally occurring antibody. In some embodiments, an antibody is purified from natural components. In some embodiments, an antibody is recombinantly produced. In some embodiments, an antibody is produced by a hybridoma.

[0133] A blocking antibody or an antagonist antibody is one which inhibits or reduces biological activity of the antigen it binds, such as FOLRI. In a certain embodiment, blocking antibodies or antagonist antibodies substantially or completely inhibit the biological activity of the antigen. Desirably, the biological activity is reduced by 10%, 20%, 30%, 50%, 70%, 80%, 90%, 95%, or even 100%.

[0134] The term anti-FOLRl antibody or an antibody that binds to FOLRI refers to an antibody that is capable of binding FOLRI with sufficient affinity such that the antibody is useful as a diagnostic and/or therapeutic agent in targeting FOLRI. Unless otherwise specified, the extent of binding of an anti-FOLRl antibody to an unrelated, nonFOLRI protein is less than about 10% of the binding of the antibody to FOLRI as measured, e.g., by a radioimmunoassay (RIA). In certain embodiments, an antibody that binds to FOLR.I has a dissociation constant (K.d) of <1 μΜ, <100 nM, <10 nM, <1 nM, or <0.1 nM. In one embodiment, the anti-FOLRl antibody does not bind FOLR2, FOLR3, FOLR4, or folic acid. Examples of FOLRI antibodies are known in the art and are disclosed in U.S.

WO 2015/031815 ״30־

Published Application Nos. 2012/0009181 and 2012/0282175 and U.S. Provisional Application Nos. 61/695,791 and 61/756,254, and PCT publication WO2011/106528, each of which is herein incorporated by reference. The sequences of anti-FOLRl antibodies and antigen-binding fragments thereof are provided in Tables 1-8.

[0135] The term antibody fragment refers to a portion of an intact antibody and refers to the antigenic determining variable regions of an intact antibody. Examples of antibody fragments include, but are not limited to, Fab, Fab1, F(ab')2, and Fv fragments, linear antibodies, single chain antibodies, and multispecific antibodies formed from antibody fragments. The term antigen-binding fragment of an antibody includes one or more fragments of an antibody that retain the ability to specifically bind to an antigen. It has been shown that the antigen-binding function of an antibody can be performed by certain fragments of a full-length antibody. Examples of binding fragments encompassed within the term antigen-binding fragment of an antibody include (without limitation): (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CHI domains (e.g., an antibody digested by papain yields three fragments: two antigen-binding Fab fragments, and one Fc fragment that does not bind antigen); (ii) a F(ab')2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region (e.g., an antibody digested by pepsin yields two fragments: a bivalent antigen-binding F(ab')2 fragment, and a pFc' fragment that does not bind antigen) and its related F(ab') monovalent unit; (iii) a Fd fragment consisting of the VH and CHI domains (i.e., that portion of the heavy chain which is included in the Fab); (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, and the related disulfide linked Fv; (v) a dAb (domain antibody) or sdAb (single domain antibody) fragment (Ward et ah, Nature 341:544-546, 1989), which consists of a VH domain; and (vi) an isolated complementarity determining region (CDR).

[0136] A monoclonal antibody refers to a homogeneous antibody population involved in the highly specific recognition and binding of a single antigenic determinant, or epitope. This is in contrast to polyclonal antibodies that typically include different antibodies directed against different antigenic determinants. The term monoclonal antibody encompasses both intact and full-length monoclonal antibodies as well as antibody fragments (such as Fab, Fab', F(ab')2, Fv), single chain (scFv) mutants, fusion proteins comprising an antibody portion, and any other modified immunoglobulin molecule comprising an antigen recognition site. Furthermore, monoclonal antibody” refers to such antibodies made in any

number of manners including but not limited to by hybridoma, phage selection, recombinant expression, and transgenic animals.

[0137] The term humanized antibody refers to forms of non-human (e.g., murine) antibodies that are specific immunoglobulin chains, chimeric immunoglobulins, or fragments thereof that contain minimal non-human (e.g., murine) sequences. Typically, humanized antibodies are human immunoglobulins in which residues from the complementary determining region (CDR) are replaced by residues from the CDR of a non-human species (e.g., mouse, rat, rabbit, hamster) that have the desired specificity, affinity, and capability (Jones et al., 1986, Nature, 321:522-525; Riechmann et al., 1988, Nature, 332:323-327; Verhoeyen et al., 1988, Science, 239:1534-1536). In some instances, the Fv framework region (FR) residues of a human immunoglobulin arc replaced with the corresponding residues in an antibody from a non-human species that has the desired specificity, affinity, and capability. The humanized antibody can be further modified by the substitution of additional residues either in the Fv framework region and/or within the replaced non-human residues to refine and optimize antibody specificity, affinity, and/or capability. In general, the humanized antibody will comprise substantially all of at least one, and typically two or three, variable domains containing all or substantially all of the CDR regions that correspond to the non-human immunoglobulin whereas all or substantially all of the FR regions are those of a human immunoglobulin consensus sequence. The humanized antibody can also comprise at least a portion of an immunoglobulin constant region or domain (Fc), typically that of a human immunoglobulin. Examples of methods used to generate humanized antibodies arc described in U.S. Pats. 5,225,539 and 5,639,641, Roguska et al., Proc. Natl. Acad. Sci., USA, 91(3):969-973 (1994), and Roguska et al., Protein Eng. 9(10):895-904 (1996). In some embodiments, a humanized antibody is a resurfaced antibody. In some embodiments, a humanized antibody is a CDR-grafted antibody .

[0138] A variable region of an antibody refers to the variable region of the antibody light chain or the variable region of the antibody heavy chain, either alone or in combination. The variable regions of the heavy and light chain each consist of four framework regions (FR) connected by three complementarity determining regions (CDRs) also known as hypervariablc regions. The CDRs in each chain arc held together in close proximity by the FRs and, with the CDRs from the other chain, contribute to the formation of the antigenbinding site of antibodies. There are at least two techniques for determining CDRs: (1) an approach based on cross-species sequence variability (i.e., Kabat et al. Sequences of Proteins of Immunological Interest, (5th cd., 1991, National Institutes of Health, Bethesda Md.)); and

(2) an approach based on crystallographic studies of antigen-antibody complexes (Al-lazikani et al (1997) J. Molec. Biol. 273:927-948)). In addition, combinations of these two approaches are sometimes used in the art to determine CDRs.

[0139] The Kabat numbering system is generally used when referring to a residue in the variable domain (approximately residues 1-107 of the light chain and residues 1-113 of the heavy chain) (e.g., Kabat et al., Sequences of Immunological Interest. 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)).

[0140] The ammo acid position numbering as in Kabat, refers to the numbering system used for heavy chain variable domains or light chain variable domains of the compilation of antibodies in Kabat ct al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991). Using this numbering system, the actual linear amino acid sequence can contain fewer or additional amino acids corresponding to a shortening of, or insertion into, a FR or CDR of the variable domain. For example, a heavy chain variable domain can include a single amino acid insert (residue 52a according to Kabat) after residue 52 of H2 and inserted residues (e.g., residues 82a, 82b, and 82c, etc. according to Kabat) after heavy chain FR residue 82. The Kabat numbering of residues can be determined for a given antibody by alignment at regions of homology of the sequence of the antibody with a standard Kabat numbered sequence. Chothia refers instead to the location of the structural loops (Chothia and Lesk J. Mol. Biol. 196:901-917 (1987)). The end of the Chothia CDR-HI loop when numbered using the Kabat numbering convention varies between H32 and H34 depending on the length of the loop (this is because the Kabat numbering scheme places the insertions at H35A and H35B; if neither 35A nor 35B is present, the loop ends at 32; if only 35A is present, the loop ends at 33; if both 35A and 35B are present, the loop ends at 34). The AbM hypervariablc regions represent a compromise between the Kabat CDRs and Chothia structural loops, and arc used by Oxford Molecular's AbM antibody modeling software.

<td></td><td> Kabat</td><td> AbM</td><td> Chothia</td>

<td> 'U</td><td></td><td></td><td> 1134־124</td>

<td> U</td><td> 1.5()-1.56</td><td> LSOTAo</td><td></td>

<td> L3</td><td> ww</td><td> I.;iUd.97</td><td> W-L97</td>

<td> Hl</td><td> HM-K35B</td><td></td><td> 1126-1132.34</td>

<td></td><td></td><td colspan="2"></td>

<td> Hl</td><td></td><td> mums</td><td> H2UH32</td>

<td></td><td></td><td colspan="2"></td>

<td></td><td> .H5i+H65</td><td> H5+HW</td><td> H52-H56</td>

<td> H3</td><td></td><td></td><td></td>

[0141] The term human antibody means an antibody produced by a human or an antibody having an amino acid sequence corresponding to an antibody produced by a human made using any technique known in the art. This definition of a human antibody includes intact or full-length antibodies, fragments thereof, and/or antibodies comprising at least one human heavy and/or light chain polypeptide such as, for example, an antibody comprising murine light chain, and human heavy chain polypeptides.

[0142] The term chimeric antibodies refers to antibodies wherein the amino acid sequence of the immunoglobulin molecule is derived from two or more species. Typically, the variable region of both light and heavy chains corresponds to the variable region of antibodies derived from one species of mammals (e.g., mouse, rat, rabbit, etc.) with the desired specificity, affinity, and capability while the constant regions are homologous to the sequences in antibodies derived from another (usually human) to avoid eliciting an immune response in that species.

[0143] The terms epitope or antigenic determinant are used interchangeably herein and refer to that portion of an antigen capable of being recognized and specifically bound by a particular antibody. When the antigen is a polypeptide, epitopes can be formed both from contiguous amino acids and noncontiguous amino acids juxtaposed by tertiary folding of a protein. Epitopes formed from contiguous amino acids arc typically retained upon protein denaturing, whereas epitopes formed by tertiary folding are typically lost upon protein denaturing. An epitope typically includes at least 3, and more usually, at least 5 or 810 amino acids in a unique spatial conformation.

[0144] Binding affinity generally refers to the strength of the sum total of noncovalent interactions between a single binding site of a molecule (e.g., an antibody) and its binding partner (e.g., an antigen). Unless indicated otherwise, as used herein, binding

&amp;#1470; affinity refers to intrinsic binding affinity which reflects a 1:1 interaction between members of a binding pair (e.g., antibody and antigen). The affinity of a molecule X for its partner Y can generally be represented by the dissociation constant (Kd) or the half-maximal effective concentration (EC50). Affinity can be measured by common methods known in the art, including those described herein. Low-affinity antibodies generally bind antigen slowly and tend to dissociate readily, whereas high-affinity antibodies generally bind antigen faster and tend to remain bound longer. A variety of methods of measuring binding affinity are known in the art, any of which can be used for purposes of the present invention. Specific illustrative embodiments are described herein.

[0145] Or better when used herein to refer to binding affinity refers to a stronger binding between a molecule and its binding partner. Or better when used herein refers to a stronger binding, represented by a smaller numerical Kd value. For example, an antibody which has an affinity for an antigen of 0.6 nM or better, the antibody's affinity for the antigen is &lt;0.6 nM, i.e., 0.59 nM, 0.58 nM, 0.57 nM etc. or any value less than 0.6 nM. In one embodiment, the antibody's affinity as determined by a Kd will be between about 10~3 to about 10’12 M, between about IO'6 to about 10 11 M, between about 106&amp;#1470; to about 10'10 M, between about 10 6 to about 1 O'9 M, between about 1045 to about 1 O'8 M, or between about 10 6 to about 107&amp;#1470; M.

[0146] The phrase substantially similar, or substantially the same, as used herein, denotes a sufficiently high degree of similarity between two numeric values (generally one associated with an antibody of the invention and the other associated with a reference/comparator antibody) such that one of skill in. the art would consider the difference between the two values to be of little or no biological and/or statistical significance within the context of the biological characteristics measured by said values (e.g., Kd values). The difference between said two values is less than about 50%, less than about 40%, less than about 30%, less than about 20%, or less than about 10% as a function of the value for the referencc/comparator antibody.

[0147] The tern immunoconjugate or conjugate as used herein refers to a compound or a derivative thereof that is linked to a cell binding agent (i.e., an anti-FOLRl antibody or fragment thereof) and is defined by a generic formula: A-L-C, wherein C = cytotoxin, L = linker, and A = cell binding agent or anti-FOLRl antibody or antibody fragment. Immunoconjugates can also be defined by the generic formula in reverse order: CL-A.

&amp;#1470; -

[0148] A linker is any chemical moiety that is capable of linking a compound, usually a drug, such as a maytansinoid, to a cell-binding agent such as an anti-FOLRl antibody or a fragment thereof in. a stable, covalent manner. Linkers can be susceptible to or be substantially resistant to acid-induced cleavage, light-induced cleavage, peptidase-induced cleavage, esterase-induced cleavage, and disulfide bond cleavage, at conditions under which the compound or the antibody remains active. Suitable linkers are well known in the art and include, for example, disulfide groups, thioether groups, acid labile groups, photolabile groups, peptidase labile groups and esterase labile groups. Linkers also include charged linkers, and hydrophilic forms thereof as described herein and know in the art.

[0149] A polypeptide, antibody, polynucleotide, vector, cell, or composition which is isolated is a polypeptide, antibody, polynucleotide, vector, cell, or composition which is in a form not found in nature. Isolated polypeptides, antibodies, polynucleotides, vectors, cells or compositions include those which have been purified to a degree that they are no longer in a form in which they are found in nature. In some embodiments, an antibody, polynucleotide, vector, cell, or composition which is isolated is substantially pure.

[0150] As used herein, substantially pure refers to material which is at least 50% pure (i.e., free from contaminants), at least 90% pure, at least 95% pure, at least 98% pure, or at least 99% pure.

[0151] The terms elevated FOLRI, increased expression of FOLRI and overexpression of FOLRI refer to a sample which contains elevated levels of FOLRI expression. The FOLRI can be elevated, increased, or overexpressed as compared to a control value (e.g., expression level in a biological sample, tissue, or cell from a subject without cancer, a sample or cancer known to express no or low FOLRI, a normal sample, or a cancer that does not have elevated FOLRI values). For example, a sample with increased expression can contain an increase of at least 2-fold, at least 3-fold, or at least 5-fold relative to a control values.

[0152] .FOLR1 expression can be measured by immunohistochemistry and given a staining intensity score or a staining uniformity score by comparison to calibrated controls exhibiting defined scores (e.g., an intensity score of 3 is given to the test sample if the intensity is comparable to the level 3 calibrated control or an intensity of 2 is given to the test sample if the intensity is comparable to the level 2 calibrated control). For example, a score of 1, 2, or 3 (3+), preferably a score of 2, or 3 (34&amp;#1470;), by immunohistochemistry indicates an increased expression of FOLRI. A staining uniformity that is heterogeneous or homogeneous is also indicative of FOLRI expression. The staining intensity and staining

&amp;#1524; - uniformity scores can be used alone or in combination (e.g., 2 homo, 2 hetero, 3 homo, 3 hetero, etc.). See Table 11. In another example, an increase in FOLRI expression can be determined by detection of an increase of at least 2-fold, at least 3-fold, or at least 5-fold relative to control values (e.g., expression level in a tissue or cell from a subject without cancer or with a cancer that does not have elevated FOLRI values).

[0153] A reference sample can be used to correlate and compare the results obtained in the methods of the invention from a test sample. Reference samples can be cells (e.g., cell lines, cell pellets) or tissue. The FOLRI levels in the reference sample can be an absolute or relative amount, a range of amount, a minimum and/or maximum amount, a mean amount, and/or a median amount of FOLRI. A reference sample can also serve as a baseline of FOLRI expression to which the test sample is compared. The reference sample can include a prior sample or baseline sample from the same patient, a normal reference with a known level of FOLRI expression, or a reference from a relevant patient population with a known level of FOLRI expression. FOLRI levels can also be expressed as values in a standard curve. A standard curve is a quantitative method of plotting assay data to determine the concentration of FOLRI in a sample. In one embodiment, a reference sample is an antigen standard comprising purified FOLRI or FOLRI-Fc. The diagnostic methods of the invention can involve a comparison between expression levels of FOLRI in a test sample and a reference value. In some embodiments, the reference value is the expression level of the FOLRI in a reference sample. A reference value can be a predetermined value and can also be determined from reference samples (e.g., control biological samples or reference samples) tested in parallel with the test samples. A reference value can be a single cut-off value, such as a median or mean or a range of values, such as a confidence interval. Reference values can be established for various subgroups of individuals, such as individuals predisposed to cancer, individuals having early or late stage cancer, male and/or female individuals, or individuals undergoing cancer therapy. Examples of normal reference samples or values and positive reference samples or values are described herein and are also described in Examples 1 and Examples 8-10 of WO 2012/135675 herein incorporated by reference.

[0154] In some embodiments, the reference sample is a sample from a healthy tissue, in particular a corresponding tissue which is not affected by cancer or a corresponding tissue which is not affected by a cancer that overexpresses FOLRI or a corresponding healthy tissue that is known not to express detectable levels of FOLR. These types of reference samples are referred to as negative control samples or normal reference samples. Tn other embodiments, the reference sample is a sample from a tumor or healthy tissue that expresses

WO 2015/031815 &amp;#1470; 37 &amp;#1470; detectable FOLRI. These types of reference samples are referred to as positive control or positive reference samples. Positive control samples can also be used as a comparative indicator for the type (hetero versus homo) and/or degree (0, 1, 2, 3) of staining intensity, which correlates with the level of FOLRI expression. Positive control comparative samples are also referred to as calibrated reference samples. Low or non-FOLRl expressing references are described herein in the Examples and also include all structures of the esophagus, acinar cells/islets of the pancreas, interalveolar connective tissue of lung, and acinar cells of the salivary gland. For cell lines, exemplary non-expre ssors include BxPC3, Panc-1, and ASPC1. Positive FOLR1 references are described herein, for example, in the Examples and also include ducts of pancreas, respiratory epithelium of normal lung, and intercalated ducts of the salivary gland. In some embodiments, positive FOLRI references include ducts of pancreas and intercalated ducts of the salivary gland. For cell lines, exemplary high FOLRI expressors are described herein, for example, in the Examples and also include KB, HeLa, 300.19 cells transfected with FOLRI, Igrov-15 and Wish, and exemplary low FOLRI expressors include Ovcar-3, Caov-3, SW620, T47D, and Skov-3. Another positive high FOLRI reference is a cell line stably or transiently transfected with FOLRI. Additional positive and negative samples for FOLRI are described in Table 13. Appropriate positive and negative reference levels of FOLRI for a particular cancer can be determined by measuring levels of FOLRI in one or more appropriate subjects, and such reference levels can be tailored to specific populations of subjects (e.g., a reference level can be age-matched so that comparisons can be made between FOLRI levels in samples from subjects of a certain age and reference levels for a particular disease state, phenotype, or lack thereof in a certain age group). Such reference levels can also be tailored to specific techniques that are used to measure levels of FOLRI in biological samples (e.g., immunoassays, etc.).

[0155] As used herein, immunohistochemistry refers to histochemical and immunologic methods used to analyze, for example, cells or tissues. Thus, the terms immunohistochemistry,” immunocytochemistry, and immunochemistry are used interchangeably.

[0156[ The term primary antibody herein refers to an antibody that binds specifically to the target protein antigen in a sample. A primary antibody is generally the first antibody used in an ELISA assay or JHC procedure. In one embodiment, the primary antibody is the only antibody used in an 1HC procedure. The term secondary antibody herein refers to an antibody that binds specifically to a primary antibody, thereby forming a

&amp;#1470; - bridge or link between the primary antibody and a subsequent reagent, if any. The secondary antibody is generally the second antibody used in an immunohistochemical procedure.

[0157] A sample or biological sample of the present invention is of biological origin, in specific embodiments, such as from eukaryotic organisms. In some embodiments, the sample is a human sample, but animal samples may also be used. Non-limiting sources of a sample for use in the present invention include solid tissue, biopsy aspirates, ascites, fluidic extracts, blood, plasma, serum, spinal fluid, lymph fluid, the external sections of the skin, respiratory, intestinal, and genitourinary tracts, tears, saliva, milk, tumors, organs, cell cultures and/or cell culture constituents, for example. A cancerous sample is a sample that contains a cancerous cell. The method can be used to examine an aspect of expression of FOLRI or a state of a sample, including, but not limited to, comparing different types of cells or tissues, comparing different developmental stages, and detecting or determining the presence and/or type of disease or abnormality.

[0158] For the purposes herein, a section of a tissue sample regards a single part or piece of a tissue sample, e.g. a thin slice of tissue or cells cut from a tissue sample. It is understood that multiple sections of tissue samples may be taken and subjected to analysis according to the present invention. In some cases, the selected portion or section of tissue comprises a homogeneous population of cells. In other cases, the selected portion comprises a region of tissue, e.g. the lumen as a non-limiting example. The selected portion can be as small as one cell or two cells, or could represent many thousands of cells, for example. In most cases, the collection, of cells is important, and while the invention has been described for use in the detection of cellular components, the method may also be used for detecting noncellular components of an organism (e.g. soluble components in the blood as a non-limiting example).

[0159] As used herein, the term capture reagent refers to a reagent capable of binding and capturing a target molecule in a sample such that under suitable condition, the capture reagent-target molecule complex can be separated from the rest of the sample. In one embodiment, the capture reagent is immobilized. In one embodiment, the capture reagent in a sandwich immunoassay is an antibody or a mixture of different antibodies against a target antigen.

[0160] As used herein, the term detectable antibody refers to an antibody that is capable of being detected cither directly through a label amplified by a detection means, or indirectly through, e.g., another antibody that is labeled. For direct labeling, the antibody is

&amp;#1493;&amp;#1497;.

&amp;#1470; - typically conjugated to a moiety that is detectable by some means. In one embodiment, the detectable antibody is a biotinylated antibody.

[0161] As used herein, the term detection means refers to a moiety or technique used to detect the presence of the detectable antibody and includes detection agents that amplify the immobilized label such as label captured onto a microtiter plate. In one embodiment, the detection means is a fluorimetric detection agent such as avidin or streptavidin.

[0162] Commonly a sandwich ELISA employs the following steps: (1) microtiter plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added and binds to antigen; (4) enzyme-linked secondary antibody is added and binds to detecting antibody; and (5) substrate is added and is converted by enzyme to detectable form.

[0163] The word label when used herein refers to a detectable compound or composition which is conjugated directly or indirectly to the antibody so as to generate a labeled antibody. The label can be detectable by itself (e.g. radioisotope labels or fluorescent labels) or, in the case of an enzymatic label, can catalyze chemical alteration of a substrate compound or composition which is detectable.

[0164] By correlate or correlating is meant comparing, in any way, the performance and/or results of a first analysis with the performance and/or results of a second analysis. For example, one may use the results of a first analysis in carrying out the second analysis and/or one may use the results of a first analysis to determine whether a second analysis should be performed and/or one may compare the results of a first analysis with the results of a second analysis. In one embodiment, increased expression of FOLRI correlates with increased likelihood of effectiveness of a FOLRI -targeting anti-cancer therapy.

[0165] The terms cancer and cancerous refer to or describe the physiological condition in mammals in which a population of cells are characterized by unregulated cell growth. Examples of cancer include, but are not limited to, carcinoma, lymphoma, blastoma, sarcoma, and leukemia. More particular examples of such cancers include cancers of endothelial, mesenchymal, or epithelial origin, such as lung cancer (e.g., squamous cell cancer, small-cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung, mesothelioma, and squamous carcinoma of the lung), cancer of the peritoneum (e.g., primary peritoneal), hepatocellular cancer, gastrointestinal cancer, pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer (serous or endometrioid), liver cancer, bladder cancer, hepatoma, breast cancer, colon cancer, colorectal cancer, endometrioid (e.g., endometrial

WO 2015/031815 &amp;#1470; 40&amp;#1470; adenocarcinoma) or uterine carcinoma, salivary gland carcinoma, kidney cancer, liver cancer, prostate cancer, vulval cancer, thyroid cancer, hepatic carcinoma, brain cancer (e.g. glioblastoma, tumors of the choroid plexus) and various types of head and neck cancers, and also tumors of blood vessels and fallopian tubes. Cancers also encompass cancers which contain cells having elevated FOLRI expression levels. Such FOLRI-elevated cancers include, but are not limited to, ovarian, non-small cell lung cancer (adenocarcinoma), uterine, endometrial, pancreatic, renal, lung, and breast cancer.

[0166] Tumor and neoplasm refer to any mass of tissue that result from excessive cell growth or proliferation, either benign (noncancerous) or malignant (cancerous) including pre-cancerous lesions.

[0167] The terms cancer cell, tumor cell, and grammatical equivalents refer to the total population of cells derived from a tumor or a pre-cancerous lesion, including both non-tumorigcnic cells, which comprise the bulk of the tumor cell population, and tumorigenic stem cells (cancer stem cells). As used herein, the term tumor cell will be modified by the term non-tumorigenic when referring solely to those tumor cells lacking the capacity to renew and differentiate to distinguish those tumor cells from cancer stem cells.

[0168] The term subject refers to any animal (e.g., a mammal), including, but not limited to humans, non-human primates, rodents, and the like, which is to be the recipient of a particular treatment. Typically, the terms subject” and patient are used interchangeably herein in reference to a human subject.

[0169] The term pharmaceutical formulation refers to a preparation which is in such form as to permit the biological activity of the active ingredient to be effective, and which contains no additional components which are unacceptably toxic to a subject to which the formulation would be administered. Such formulation can be sterile.

[0170] An effective amount of an antibody or immunoconjugatc as disclosed herein is an amount sufficient to carry out a specifically stated purpose. An effective amount can be determined empirically and in a routine manner, in relation to the stated purpose.

[0171] The term therapeutically effective amount refers to an amount of an antibody or other drug effective to treat a disease or disorder in a subject or mammal. In the case of cancer, the therapeutically effective amount of the drug can reduce the number of cancer cells; reduce the tumor size; inhibit (i.e., slow to some extent and in a certain embodiment, stop) cancer cell infiltration into peripheral organs; inhibit (i.e., slow to some extent and in. a certain embodiment, stop) tumor metastasis; inhibit, to some extent, tumor growth; relieve to some extent one or more of the symptoms associated with the cancer;

WO 2015/031815 * 41 &amp;#1470;&amp;#1470; and/or result in a favorable response such as increased progression-free survival (PFS), disease-free survival (DFS), or overall survival (OS), complete response (CR), partial response (PR), or, in some cases, stable disease (SD), a decrease in progressive disease (PD), a reduced time to progression (TTP), a decrease in CA125 in the case of ovarian cancer, or any combination thereof. See the definition herein of treating. To the extent the drug can prevent growth and/or kill existing cancer cells, it can be cytostatic and/or cytotoxic. In certain embodiments, identification of increased FOLRI levels allows for administration of decreased amounts of the FOLRI-targeting therapeutic to achieve the same therapeutic effect as seen with, higher dosages. A prophylactically effective amount refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired prophylactic result. Typically but not necessarily, since a prophylactic dose is used in subjects prior to or at an earlier stage of disease, the prophylactically effective amount will be less than the therapeutically effective amount.

[0172] The term respond favorably generally refers to causing a beneficial state in a subject. With respect to cancer treatment, the term refers to providing a therapeutic effect on the subject. Positive therapeutic effects in cancer can be measured in a number of ways (See, W.A. Weber, J. Nucl. Med. 5O:1S-1OS (2009)). For example, tumor growth inhibition, molecular marker expression, serum marker expression, and molecular imaging techniques can all be used to assess therapeutic efficacy of an anti-cancer therapeutic. With respect to tumor growth inhibition, according to NCI standards, a T/C &lt; 42% is the minimum level of anti-tumor activity. A T/C &lt;10% is considered a high anti-tumor activity level, with T/C (%) &amp;#1470; Median tumor volume of the treated / Median tumor volume of the control x 100. A favorable response can be assessed, for example, by increased progression-free survival (PFS), disease-free survival (DFS), or overall survival (OS), complete response (CR), partial response (PR), or, in. some cases, stable disease (SD), a decrease in progressive disease (PD), a reduced time to progression (TTP), a decrease in CAI25 in the case of ovarian cancer or any combination thereof.

[0173] PFS, DFS, and OS can be measured by standards set by the National Cancer

Institute and the U.S. Food and Drug Administration for the approval of new drugs. Sec

Johnson et al, (2003) J. Clin. Oncol. 21(7):1404-14.11.

[0174] Progression free survival (PFS) refers to the time from enrollment to disease progression or death. PFS is generally measured using the Kaplan-Meier method and

Response Evaluation Criteria in. Solid Tumors (REC1ST) 1.1 standards. Generally,

WO 2015/031815 &amp;#1470; 42&amp;#1470; progression free survival refers to the situation wherein a patient remains alive, without the cancer getting worse.

[0175] Time to Tumor Progression (TTP) is defined as the time from enrollment to disease progression. TTP is generally measured using the RECIST 1.1 criteria.

[0176] A complete response or complete remission or CR indicates the disappearance of all signs of tumor or cancer in response to treatment. This does not always mean the cancer has been cured.

[0177] A partial response or PR refers to a decrease in the size or volume of one or more tumors or lesions, or in the extent of cancer in the body, in response to treatment.

[0178] Stable disease refers to disease without progression or relapse. In stable disease there is neither sufficient tumor shrinkage to qualify for partial response nor sufficient tumor increase to qualify as progressive disease.

[0179] Progressive disease refers to the appearance of one more new lesions or tumors and/or the unequivocal progression of existing non-target lesions. Progressive disease can also refer to a tumor growth of more than 20 percent since treatment began, either due to an increases in mass or in spread of the tumor.

[0180] Disease free survival (DFS) refers to the length of time during and after treatment that the patient remains free of disease.

[0181] Overall Survival (OS) refers to the time from patient enrollment to death or censored at the date last known alive. OS includes a prolongation in life expectancy as compared to naive or untreated individuals or patients. Overall survival refers to the situation wherein a patient remains alive for a defined period of time, such as one year, five years, etc., e.g., from the time of diagnosis or treatment.

[0182] A decrease in CAI25 levels can be assessed according to the Gynecologic Cancer Intergroup (GC1G) guidelines. For example, CAI25 levels can be measured prior to treatment to establish a baseline CA.125 level. CA125 levels can be measured one or more times during or after treatment, and a reduction in the CA125 levels over time as compared to the baseline level is considered a decrease in CA125 levels.

[0183] Terms such as treating or treatment or to treat or alleviating or to alleviate refer to therapeutic measures that cure, slow down, lessen symptoms of, and/or halt progression of a diagnosed pathologic condition or disorder. Thus, those in need of treatment include those already diagnosed with or suspected of having the disorder. In certain embodiments, a subject is successfully treated for cancer according to the methods of the present invention if the patient shows one or more of the following: a reduction, in the number

WO 2015/031815 “43 - of or complete absence of cancer cells; a reduction in the tumor size; inhibition of or an absence of cancer cell infiltration into peripheral organs including, for example, the spread of cancer into soft tissue and bone; inhibition of or an absence of tumor metastasis; inhibition or an absence of tumor growth; relief of one or more symptoms associated with the specific cancer; reduced morbidity and mortality; improvement in quality of life; reduction in tumorigenicity, tumorigenic frequency, or tumorigenic capacity, of a tumor; reduction in the number or frequency of cancer stem cells in a tumor; differentiation of tumorigenic cells to a non-tumorigenic state; increased progression-free survival (PFS), disease-free survival (DFS), or overall survival (OS), complete response (CR), partial response (PR), stable disease (SD), a decrease in progressive disease (PD), a reduced time to progression (TTP), a decrease in CAI 25 in the case of ovarian cancer, or any combination thereof.

[0184] Prophylactic or preventative measures refer to therapeutic measures that prevent and/or slow the development of a targeted pathologic condition or disorder. Thus, those in need of prophylactic or preventative measures include those prone to have the disorder and those in whom the disorder is to be prevented.

[0185] As used herein, the term healthcare provider refers to individuals or institutions which directly interact with and administer to living subjects, e.g., human patients. Non-limiting examples of healthcare providers include doctors, nurses, technicians, therapist, pharmacists, counselors, alternative medicine practitioners, medical facilities, doctor’s offices, hospitals, emergency rooms, clinics, urgent care centers, alternative medicine clinics/facilitics, and any other entity providing general and/or specialized treatment, assessment, maintenance, therapy, medication, and/or advice relating to all, or any portion of, a patient’s state of health, including but not limited to general medical, specialized medical, surgical, and/or any other type of treatment, assessment, maintenance, therapy, medication and/or advice.

|0186] In some aspects, a healthcare provider can administer or instruct another healthcare provider to administer a therapy to treat a cancer. Administration of a therapy, as used herein, includes prescribing a therapy to a subject as well as delivering, applying, or giving the therapy to a subject. A healthcare provider can implement or instruct another healthcare provider or patient to perform the following actions: obtain a sample, process a sample, submit a sample, receive a sample, transfer a sample, analyze or measure a sample, quantify a sample, provide the results obtained after analyzing/mcasuring/quantifying a sample, receive the results obtained after analyzing/measuring/quantifying a sample, compare/score the results obtained after analyzing/measuring/quantifying one or more &amp;#1524;:vl —_™—___^——

WO 2015/031815 &amp;#1470; 44 &amp;#1470; samples, provide the comparison/score from one or more samples, obtain the comparison/score from one or more samples, administer a therapy or therapeutic agent (e.g, a FOLRI binding agent), commence the administration of a therapy, cease the administration of a therapy, continue the administration of a therapy, temporarily interrupt the administration of a therapy, increase the amount of an administered therapeutic agent, decrease the amount of an administered therapeutic agent, continue the administration of an amount of a therapeutic agent, increase the frequency of administration of a therapeutic agent, decrease the frequency of administration of a therapeutic agent, maintain the same dosing frequency on a therapeutic agent, replace a therapy or therapeutic agent by at least another therapy or therapeutic agent, combine a therapy or therapeutic agent with at least another therapy or additional therapeutic agent. These actions can be performed by a healthcare provider automatically using a computer-implemented method (e.g., via a web service or stand-alone computer system).

[0187] Polynucleotide or nucleic acid, as used interchangeably herein, refer to polymers of nucleotides of any length, and include DNA and RNA. The nucleotides can be deoxyribonucleotides, ribonucleotides, modified nucleotides or bases, and/or their analogs, or any substrate that can be incorporated into a polymer by DNA or RNA polymerase. A polynucleotide can comprise modified nucleotides, such as methylated nucleotides and their analogs. If present, modification to the nucleotide structure can be imparted before or after assembly of the polymer. The sequence of nucleotides can be interrupted by non-nucleotide components. A polynucleotide can be further modified after polymerization, such as by conjugation with a labeling component. Other types of modifications include, for example, caps, substitution of one or more of the naturally occurring nucleotides with an analog, intemucleotide modifications such as, for example, those with uncharged linkages (e.g., methyl phosphonates, phosphotricsters, phosphoamidates, cabamatcs, etc.) and with charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), those containing pendant moieties, such as, for example, proteins (e.g., nucleases, toxins, antibodies, signal peptides, ply-L-lysine, etc.), those with intercalators (e.g., acridine, psoralen, etc.), those containing chelators (e.g., metals, radioactive metals, boron, oxidative metals, etc.), those containing alkylators, those with modified linkages (e.g., alpha anomeric nucleic acids, etc.), as well as unmodified forms of the polynucleotide(s). Further, any of the hydroxyl groups ordinarily present in the sugars can be replaced, for example, by phosphonate groups, phosphate groups, protected by standard protecting groups, or activated to prepare additional linkages to additional nucleotides, or can be conjugated to solid supports. The 5' and 3' terminal OH can

&amp;#1470; be phosphorylated or substituted with amines or organic capping group moieties of from 1 to 20 carbon atoms. Other hydroxyls can also be derivatized to standard protecting groups. Polynucleotides can also contain analogous forms of ribose or deoxyribose sugars that arc generally known in the art, including, for example, 2'-O-methyl-, 2’-O-allyl, 2'-fluoro- or 2'azido-ribose, carbocyclic sugar analogs, alpha-anomeric sugars, epimeric sugars such as arabinose, xyloses or lyxoses, pyranose sugars, furanose sugars, sedoheptuloses, acyclic analogs and abasic nucleoside analogs such as methyl riboside. One or more phosphodiester linkages can be replaced by alternative linking groups. These alternative linking groups include, but are not limited to, embodiments wherein phosphate is replaced by P(O)S (thioate), P(S)S (dithioatc), (O)NR2 (amidate), P(O)R, P(O)OR', CO or CH2 (formacetal), in which each R or R' is independently H or substituted or unsubstituted alkyl (1-20 C) optionally containing an ether (—0—) linkage, aryl, alkenyl, cycloalkyl, cycloalkenyl or araldyl. Not all linkages in a polynucleotide need be identical. The preceding description applies to all polynucleotides referred to herein, including RNA and DNA.

[0188] The term vector means a construct, which is capable of delivering, and expressing, one or more gene(s) or sequence(s) of interest in a host cell. Examples of vectors include, but are not limited to, viral vectors, naked DNA or RNA expression vectors, plasmid, cosmid or phage vectors, DNA or RNA expression vectors associated with cationic condensing agents, DNA or RNA expression vectors encapsulated in liposomes, and certain eukaryotic cells, such as producer cells.

[0189] The terms polypeptide, peptide, and protein are used interchangeably herein to refer to polymers of amino acids of any length. The polymer can be linear or branched, it can comprise modified amino acids, and it can be interrupted by non-amino acids. The terms also encompass an amino acid polymer that has been modified naturally or by intervention; for example, disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation or modification, such as conjugation with a labeling component. Also included within the definition are, for example, polypeptides containing one or more analogs of an amino acid (including, for example, unnatural amino acids, etc.), as well as other modifications known in the art. It is understood that, because the polypeptides of this invention arc based upon antibodies, in certain embodiments, the polypeptides can occur as single chains or associated chains. In some embodiments, a polypeptide, peptide, or protein is non-naturally occurring. In some embodiments, a polypeptide, peptide, or protein is purified from other naturally occurring components. In some embodiments, the polypeptide, peptide, or protein is recombinantly produced.

&amp;#1470; -

[0190] The terms identical or percent identity” in the context of two or more nucleic acids or polypeptides, refer to two or more sequences or subsequences that are the same or have a specified percentage of nucleotides or amino acid residues that are the same, when compared and aligned (introducing gaps, if necessary) for maximum correspondence, not considering any conservative amino acid substitutions as part of the sequence identity. The percent identity can be measured using sequence comparison software or algorithms or by visual inspection. Various algorithms and software are known in the art that can be used to obtain alignments of amino acid or nucleotide sequences. One such non-limiting example of a sequence alignment algorithm is the algorithm described in Karlin ct al, 1990, Proc. Natl. Acad. Sci., 87:2264-2268, as modified in Karlin et al., 1993, Proc. Natl. Acad. Sci., 90:5873-5877, and incorporated into the NBLAST and XBLAST programs (Altschul et al., 1991, Nucleic Acids Res., 25:3389-3402). In certain embodiments, Gapped BLAST can be used as described in Altschul et al., 1997, Nucleic Acids Res. 25:3389-3402. BLAST-2, WUBLAST-2 (Altschul et al., 1996, Methods in Enzymology, 266:460-480), ALIGN, ALIGN-2 (Genentech, South San Francisco, California) or Megalign (DNASTAR) are additional publicly available software programs that can be used to align sequences. In certain embodiments, the percent identity between two nucleotide sequences is determined using the GAP program in GCG software (e.g., using a NWSgapdna.CMP matrix and a gap weight of 40, 50, 60, 70, or 90 and a length weight of 1, 2, 3, 4, 5, or 6). In certain alternative embodiments, the GAP program in the GCG software package, which incorporates the algorithm of Needleman and Wunsch (J. Mol. Biol. (48):444-453 (1970)) can be used to determine the percent identity between two amino acid sequences (e.g., using either a Blossum 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5). Alternatively, in certain embodiments, the percent identity between nucleotide or amino acid sequences is determined using the algorithm of Myers and Miller (CABIOS, 4:11-17 (1989)). For example, the percent identity can be determined using the ALIGN program (version 2.0) and using a PAM120 with residue table, a gap length penalty of 12 and a gap penalty of 4. Appropriate parameters for maximal alignment by particular alignment software can be determined by one skilled in the art. In certain embodiments, the default parameters of the alignment software are used. In certain embodiments, the percentage identity X of a first amino acid sequence to a second sequence amino acid is calculated as 100 x (Y/Z), where Y is the number of amino acid residues scored as identical matches in the alignment of the first and second sequences (as aligned by visual inspection or a particular sequence alignment program) and Z is the total

WO 2015/031815 &amp;#1470; 47&amp;#1470; number of residues in the second sequence. If the length of a first sequence is longer than the second sequence, the percent identity of the first sequence to the second sequence will be longer than the percent identity of the second sequence to the first sequence.

(0191] As a non-limiting example, whether any particular polynucleotide has a certain percentage sequence identity (e.g., is at least 80% identical, at least 85% identical, at least 90% identical, and in some embodiments, at least 95%, 96%, 97%, 98%, or 99% identical) to a reference sequence can, in certain embodiments, be determined using the Bestfit program (Wisconsin Sequence Analysis Package, Version 8 for Unix, Genetics Computer Group, University Research Park, 575 Science Drive, Madison, WI 53711). Bestfit uses the local homology algorithm of Smith and Waterman, Advances in Applied Mathematics 2: 482 489 (1981), to find the best segment of homology between two sequences. When using Bestfit or any other sequence alignment program to determine whether a particular sequence is, for instance, 95% identical to a reference sequence according to the present invention, the parameters are set such that the percentage of identity is calculated over the full length of the reference nucleotide sequence and that gaps in homology of up to 5% of the total number of nucleotides in the reference sequence are allowed.

[0192] In some embodiments, two nucleic acids or polypeptides of the invention are substantially identical, meaning they have at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, and in some embodiments at least 95%, 96%, 97%, 98%, 99% nucleotide or amino acid residue identity, when compared and aligned for maximum correspondence, as measured using a sequence comparison algorithm or by visual inspection. In certain embodiments, identity exists over a region of the sequences that is at least about 10, about 20, about 40-60 residues in length or any integral value therebetween, or over a longer region than 60-80 residues, at least about 90-100 residues, or the sequences arc substantially identical over the full length of the sequences being compared, such as the coding region of a nucleotide sequence for example.

[0193] A conservative amino acid substitution is one in which one amino acid residue is replaced with another amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art, including basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., glycine, alanine, valine, leucine, isolcucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine,

WO 2015/031815 &amp;#1470; 48 &amp;#1470; isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). For example, substitution of a phenylalanine for a tyrosine is a conservative substitution. In certain embodiments, conservative substitutions in the sequences of the polypeptides and antibodies of the invention do not abrogate the binding of the polypeptide or antibody containing the amino acid sequence, to the antigen(s), i.e., the FOLRI to which the polypeptide or antibody binds. Methods of identifying nucleotide and amino acid conservative substitutions which do not eliminate antigen-binding are well- known in the art (see, e.g., Brummell et al., Biochem. 32: 1180-1 187 (1993); Kobayashi et al. Protein Eng. 12(10):879-884 (1999); and Burks et al. Proc. Natl. Acad. Sci. USA 94:.412-417 (1997)).

[0194] As used in the present disclosure and claims, the singular forms a, an, and the” include plural forms unless the context clearly dictates otherwise.

[0195] It is understood that wherever embodiments are described herein with the language comprising, otherwise analogous embodiments described in terms of consisting of and/or consisting essentially of are also provided.

[0196] The term and/or as used in a phrase such as A and/or B herein is intended to include both A and B, A or B, A, and B. Likewise, the term and/or as used in a phrase such as A, B, and/or C is intended to encompass each of the following embodiments: A, B, and C; A, B, or C; A or C; A or B; B or C; A and C; A and B; B and C; A (alone); B (alone); and C (alone).

II. FOLRl-binding agents

[0197] The present invention provides agents that specifically bind human FOLRI. These agents are referred to herein as FOLRl-binding agents. In certain embodiments, the FOLRI binding agents are antibodies, immuno conjugates or polypeptides. The amino acid and nucleotide sequences for human FOLRI are known in the art and arc also provided herein as represented by SEQ ID NO: 1 and SEQ ID NO:2.

Human Folate Receptor 1:

MAQRMTTQLLLLLVWVAVVGEAQTRIAWARTELLNVCMNAKHHKEKPGPEDKLH

EQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACKRHF1QDTCLYEC

SPNLGPWIQQVDQSWRKERVLNVPLCKEDCEQWWEDCRTSYTCKSNWHKGWNWT

SGFNKCAVGAACQPFHFYFPTPTVLCNEIWTHSYKVSNYSRGSGRCIQMWFDPAQG

NPNEEVARFYAAAMSGAGPWAAWPFLLSLALMLLWLLS (SEQ ID NO: 1)

&amp;#1470; -

Human Folate Receptor 1 Nucleic Acid Sequence:

atggctcagcggatgacaacacagctgctgctccttctagtgtgggtggctgtagtaggggaggctcagacaaggattgcatgggcc aggactgagcttctcaatgtctgcatgaacgccaagcaccacaaggaaaagccaggccccgaggacaagttgcatgagcagtgtcg accctggaggaagaatgcctgctgttctaccaacaccagccaggaagcccataaggatgtttcctacctatatagattcaactggaacc actgtggagagatggcacctgcctgcaaacggcatttcatccaggacacctgcctctacgagtgctcccccaacttggggccctggat ccagcaggtggatcagagctggcgcaaagagcgggtactgaacgtgcccctgtgcaaagaggactgtgagcaatggtgggaagat tgtcgcacctcctacacctgcaagagcaactggcacaagggctggaactggacttcagggtttaacaagtgcgcagtgggagctgcc tgccaacctttccatttctacttccccacacccactgttctgtgcaatgaaatctggactcactcctacaaggtcagcaactacagccgag ggagtggccgctgcatccagatgtggttcgacccagcccagggcaaccccaatgaggaggtggcgaggttctatgctgcagccatg agtggggctgggccctgggcagcctggcctttcctgcttagcctggccctaatgctgctgtggctgctcagc (SEQ ID NO:2) [0198] Thus, in some embodiments, the FOLRI binding agents can bind to an epitope of SEQ IDNO:1.

[0199] In some embodiments, an anti-FOLRl antibody can specifically binds to an epitope of FOLRI (SEQ IDNO:1), wherein epitope comprises an N-glycosylated amino acid. Such antibodies will therefore bind to FOLRI when it is glycosylated and will not bind to FOLRI when it is not glycosylated. In other words, the binding of these antibodies is glycoldependent. These antibodies are advantageous in that they can be used to distinguish between glycosylated and non-glycosylated forms of FOLRI. Given that glycosylation can be required for membrane localization, the antibodies can advantageously be used for membrane specific staining.

[0200] In some embodiments, the anti-FOLRl antibody can specifically bind to an epitope of FOLRI comprising N-glycosylated amino acid 69 of FOLRI. In some embodiments, the anti-FOLRl antibody can specifically bind to an epitope of FOLRI comprising N-glycosylated amino acid 161 of FOLRI. In some embodiments, the antiFOLRI antibody can specifically bind to an epitope of FOLRI comprising N-glycosylated amino acid 201 of FOLRL

[0201] In certain embodiments, the anti-FOLRl antibody is the antibody produced by the hybridoma deposited with the American Type Culture Collection (ATCC), located at 10801 University Boulevard, Manassas, VA 20110 on April 16, 2013 under the terms of the Budapest Treaty and having ATCC deposit no. PTA-120196 (FOLRI-9.20, also referred to as IMGN 353.9-20, 353.9-20,&amp;#1524; or &amp;#1524;9.20&amp;#1524;). In certain embodiments, the anti-FOLRl antibody is the antibody produced by the hybridoma deposited with the ATCC on April 16, 2013 and having ATCC deposit no. PTA-120197 (FO.LR 1-2.1, also referred to as IMGN 353.2-1, 353.2-1, 2.1, or muFRIHC2-l).

(0202] The FOLRI-binding agents include FOLRI-binding agents that comprise the heavy and light chain CDR sequences of (i) muFRIHC2-l, which is also known as FOLRI 2.1, IMGN 353.2-1, 353.2-1, or 2.1, (ii) muFRJHC5-7, which is also known as IMGN 353.5-7, ”353.5-7 or 5.7, (iii) muFRIHC9-20, which is also known as FOLRI-9.20, IMGN 353.9-20, 353.9-20, or 9.20, (iv) resurfaced huFRIHC2-l version 1.0 or 1.01, or (v) CDR grafted huFRIHC2-l version 1.0 or 1.01, which are provided in Tables 1 and 2 below. The FOLRI-binding agents also include FOLRI-binding agents that comprise the heavy and light chain CDR sequences of the composite CDRs provided in Tables 1 and 2 below.

Tabic 1: Variable heavy chain CDR amino acid sequences

<td> Antibody</td><td> VH-CDR1 </td><td> - . . VH-CDR2</td><td> VH-CDR3</td>

<td> muFRIHC2-l (2.1)</td><td> NSYIH (SEQ IDNO;3)</td><td> WIYPESLNTQYNEKFKA (SEQ ID NO:4)</td><td> RGIYYYSPYALDH (SEQ ID NO:5)</td>

<td> muFRlHC5-7 (5.7)</td><td> NYYIH (SEQ ID NO:9)</td><td> WTYPGSFNVEYNEKFKA (SEQ ID NO :10)</td><td> RGIYFYSPYALDY (SEQ ID NO:11)</td>

<td> muFRIHC9-20 (9.20)</td><td> NYYIH (SEQ ID NO:15)</td><td> WIYPENVNVRYNDKFKA (SEQ ID NO:16)</td><td> RGIYYYSPYAMDY (SEQ ID NO: 17)</td>

<td> Composite</td><td> N(Y/S)YIH (SEQ ID NO:21)</td><td> WIYP(G/E)(S/N)(F/V/L)N(V/ T)(E/R/Q)YN(E/D)KFKA (SEQ ID NO :22)</td><td> RGIY(F/Y)YSPYA(L/ M)D(Y/H) (SEQ ID NO:23)</td>

Table 2: Variable light chain CDR amino acid sequences

<td> Antibody</td><td> VL-CDR1</td><td> VL-CDR2</td><td> VL-CDR3</td>

<td> muFR!HC2-l (2.1)</td><td> KSSKSLLNSDGFTYLD (SEQ ID NO:6)</td><td> LVSNHFS (SEQ ID NO:7)</td><td> FQSNYLPLT (SEQ ID NO :8)</td>

<td> muFRIHC5-7 (5.7)</td><td> KSTESLLNSDG FTYLD (SEQ ID NO;12)</td><td> LVSNHFS (SEQ ID NO.-13)</td><td> FQSNYLPLT (SEQ ID NO :14)</td>

<td> muFRlHC9-20 (9.20)</td><td> KSTKSLLNSDGFTYLD (SEQ ID NO: 18)</td><td> LVSNHFS (SEQ ID NO :19)</td><td> FQSNYLPLT (SEQ ID NO :20)</td>

<td> Composite</td><td> KS(T/S)(K/E)SLLNSDGFTY LD (SEQ ID NO:24)</td><td> LVSNHFS (SEQ ID NO:25)</td><td> FQSNYLPLT (SEQ ID NO :26)</td>

_ !5&amp;#1524;

[0203] The FOLRI binding molecules can be antibodies or antigen binding fragments that specifically bind to FOLRI that comprise the CDRs of antibody 2.1 (i.e., SEQ ID NOs: 3-8), 5.7 (i.e., SEQ ID NOs: 9-14), or 9.20 (i.e., SEQ ID NOs: 15-20), with up to four (i.e., 0, 1, 2, 3, or 4) conservative amino acid substitutions per CDR. The FOLRI binding molecules can be antibodies or antigen-binding fragments that specifically bind to FOLRI that comprise the CDRs of the composite sequence shown above (i.e., SEQ ID NOs: 21-26), with up to four (i.e., 0, 1,2, 3, or 4) conservative amino acid substitutions per CDR.

[0204] The FOLRI binding molecules can be antibodies or anti gen-binding fragments that specifically bind to FOLRI that comprise the CDRs of antibody produced by the hybridoma of ATCC deposit no. PTA-120196 or PTA-120197.

[0205] Polypeptides can .comprise one of the individual variable light chains or variable heavy chains described herein. Antibodies and polypeptides can also comprise both a variable light chain and a variable heavy chain. The variable light chain and variable heavy chain sequences of murine antibodies 2.1, 5.7, and 9.20 and humanized 2.1 are provided in Tables 3 and 4 below.

Table 3: Variable heavy chain amino acid sequences

<td> Antibody</td><td> VH Amino Acid Sequence (SEQ ID NO)</td>

<td> muFRIHC2-l (2.1)</td><td> QVQLQQSGPELVKPGASVRISCKASGYTFTNSYIHWVKKRPGQGL EWIGWIYPESLNTQYNEKFKAKATLTADKSSSTSYMQLSSLTSEDS AVYFCARRGIYYYSPYALDHWGQGASVTVSS (SEQ ID NO:27)</td>

<td> muFRIHC5-7 (5.7)</td><td> QVQLQQSGPEVVKPGASVRISCKASGYTFTNYYIHWVKQRPGQGL EW1GWIYPGSFNVEYNEKFKAKATLTADKSSSTVYMQLSSLTSEDS AA^YFCARRGIYFYSPYALDYWGQGASVTVSS (SEQ ID NO:29)</td>

<td> muFRlHC9-20 (9.20)</td><td> QVQLQQSGPDLVKPGASVRISCKASGFTFTNYYIHWVKQRPGQGL EWIGWIYPENVNVRYNDKFKAKATLTADKSSSTAYMQLSSLTSED SAVYFCARRGTYYYSPYAMDYWGQGASVTVSS (SEQ ID NO:31)</td>

<td> huFRIHC2-l (resurfaced)</td><td> QVQLVQSGAEWKPGASVKISCKASGYTFTNSYIHWVKKRPGQGL EWIGWIYPESLNTQYNQKFQGKATLTADKSSSTSYMQLSSLTSEDS AVYFCARRGIYYYSPYALDHWGQGASVTVSS (SEQ ID NO:62)</td>

<td> huFRIHC2-l (grafted)</td><td> QVQLVQSGAEVKKPGASVKVSCKASGYTFTNSYIHWVRQAPGQG LEWMGWIYPESLNTQYNEKFKARVTMTRDTS1STAYMELSRLRSD DTAVYYCARRGIYYYSPYALDHWGQGTLVTVSSAST (SEQ ID NO:65)</td>

WO 2015/031815 &amp;#1470; 52 &amp;#1470;

Table 4: Variable light chain amino acid sequences

<td> Antibody</td><td> VL Amino Acid Sequence (SEQ ID NO)</td>

<td> muFRIHC2-l (2.1)</td><td> SDVVLTQTPLSLPVNIGDQASISCKSSKSLLNSDGFTYLDWYLQKPG QSPQLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYC FQSNYLPLTFGGGTKLEIKR (SEQ ID NO:28)</td>

<td> muFRIHC5-7 (5.7)</td><td> SDWLTQTPLSLPVNIGDQASISCKSTESLLNSDGFTYLDWYLQKPG QSPQLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYC FQSNYLPLTFGGGTKLEVKR (SEQ ID NO :30)</td>

<td> muFR!HC9-20 (9.20”)</td><td> SDWLTQTPLSLPVNLGDQASISCKSTKSLLNSDGFTYLDWYLQKP GQSPQLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYY CFQSNYLPLTFGGGTKLEIKR (SEQ ID NO:32)</td>

<td> huFRIHC2-l v. 1.0 (resurfaced)</td><td> DWLTQSPLSLPVNLGQPASISCRSSRSLLNSDGFTYLDWYLQKPGQ SPRLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCF QSNYLPLTFGQGTKLEIKR (SEQ ID NO:63)</td>

<td> huFRIHC2-l v. 1.01 (resurfaced)</td><td> DVVLTQSPLSLPVNLGQPASISCKSSKSLLNSDGFTYLDWYLQKPG QSPRLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYC FQSNYLPLTFGQGTKLEIKR (SEQ ID NO :64)</td>

<td> huFRIHC2-l v. 1.0 (grafted)</td><td> DIVMTQTPLSLSVTPGQPASISCRSSRSLLNSDGFTYLDWYLQKPGQ SPQLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCF QSNYLPLTFGQGTKLEIK (SEQ ID NO:66)</td>

<td> huFRIHC2-l v. 1.01 (grafted)</td><td> DIVMTQTPLSLSVTPGQPASISCKSSKSLLNSDGFTYLDWYLQKPGQ SPQLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCF QSNYLPLTFGQGTKLEIK (SEQ ID NO:67)</td>

[0206] Also provided are polypeptides that comprise: (a) a polypeptide having at least about 90% sequence identity to SEQ ID NOs:27, 29, 31, 62, or 65; and/or (b) a polypeptide having at least about 90% sequence identity to SEQ ID NOs:28, 30, 32, 63, 64, 66, or 67. In certain embodiments, the polypeptide comprises a polypeptide having at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% sequence identity to SEQ ID NOs:2732&amp;#1470; or 62-67. Thus, in certain embodiments, the polypeptide comprises (a) a polypeptide having at least about 95% sequence identity to SEQ ID NOs:27, 29, 31, 62, or 65 and/or (b) a polypeptide having at least about 95% sequence identity to SEQ ID NOs:28, 30, 32, 63, 64, 66, or 67. In certain embodiments, the polypeptide comprises (a) a polypeptide having the amino acid sequence of SEQ ID NOs:27, 29, 31, 62, or 65; and/or (b) a polypeptide having the amino acid sequence of SEQ ID NOs:28, 30, 32, 63, 64, 66, or 67. in certain embodiments, the polypeptide is an antibody and/or the polypeptide specifically binds FOLRI. In certain embodiments, the polypeptide is a murine, chimeric, or humanized antibody that specifically binds FOLRI. In certain embodiments, the polypeptide

WO 2(115/031815 53 &amp;#1470; having a certain percentage of sequence identity to SEQ ID NOs:27-32 or 62-67 differs from SEQ ID NOs:27-32 or 62-67 by conservative amino acid substitutions only.

[0207] Also provided are polypeptides comprising a variable light chain that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical to the variable light chain sequence of the antibody produced by the hybridoma having ATCC deposit no. PTA-120196 or PTA-120197.

[0208] Also provided are polypeptides comprising a variable heavy chain that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical to the variable heavy chain sequence of the antibody produced by the hybridoma having ATCC depositno. PTA-120196 or PTA-120197.

[0209] Also provided are' antibodies and antigen-binding fragments thereof comprising variable heavy and variable light chain sequences that are at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or identical to the variable heavy and variable light chain sequences of the antibody produced by the hybridoma having ATCC deposit no. PTA-120196 or PTA-120197.

[0210] In certain embodiments, the antibody or antigen-binding fragment is the antibody produced by the hybridoma having ATCC deposit no. PTA-120196 or an antigenbinding fragment thereof.

[0211] In certain embodiments, the antibody or antigen-binding fragment is the antibody produced by the hybridoma having ATCC deposit no. PTA-120197 or an antigenbinding fragment thereof.

[0212] Polypeptides can comprise one of the individual light chains or heavy chains described herein. Antibodies and polypeptides can also comprise both a light chain and a heavy chain. The light chain and heavy chain sequences of antibodies 2.1, 5.7, and 9.20 are provided in Tables 5 and 6 below.

Table 5: Full-length heavy chain amino acid sequences

<td> Antibody</td><td> Full-Length Heavy Chain Amino Acid Sequence (SEQ ID NO)</td>

<td> muFRIHC2-l (2.1)</td><td> QVQLQQSGPELVKJPGASVRISCKASGYTFTNSYIHWVKKRPGQGLE WIGWIYPESLNTQYNEKFKAKATLTADKSSSTSYMQLSSLTSEDSAV YFCARRGIYYYSPYALDHWGQGASVTVSSAKTTPPSVYPLAPGSAA QTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLESDLYT LSSSVTVPSSMRPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTV PEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDV EVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAJFP APIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDIT VEWQWNGQPAENYKNTQPIMNTNGSYFVYSKLNVQKSNWEAGNT</td>

<td></td><td> FTCSVLHEGLHNHHTEKSLSHSPGK (SEQ ID NO:33)</td>

<td> muFRIHC5-7 (5.7)</td><td> QVQLQQSGPEWKPGASVRISCKASGYTFTNYYIHWVKQRPGQGLE W1GWIYPGSFNVEYNEKFKAKATLTADKSSSTVYMQLSSLTSEDSA VYFCARRGIYFYSPYALDYWGQGASVTVSSAKTTPPSVYPLAPGSA AQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLESDLY TLSSSVTVPSSMRPSETVTCNVAHPASSTKVDKKTVPRDCGCKPCICT VPEVSSVFIFPPKPKDVLTITLTPKVTCVWDISKDDPEVQFSWFVDD VEVHIAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAA FPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPE DITVEWQWNGQPAENYKNTQPIMNTNGSYFVYSKLNVQKSNWEAG NTFTCSVLHEGLHNHHTEKSLSHSPGK (SEQ ID NO:35)</td>

<td> muFRIHC9-20 (9.20)</td><td> QVQLQQSGPDLVKPGASVRISCKASGFTFTNYYIHWVKQRPGQGLE WIGWIYPENVNVRYNDKFKAKATLTADKSSSTAYMQLSSLTSEDSA VYFCARRGIYYYSPYAMDYWGQGASVTVSSAKTTPPSVYPLAPGSA AQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLESDLY TLSSSVTVPSSMRPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICT VPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDD VEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAA FPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPE DITVEWQWNGQPAENYKNTQPIMNTNGSYFVYSKLNVQKSNWEAG NTFTCSVLHEGLHNHHTEKSLSHSPGK (SEQ ID NO:37)</td>

<td> muhuM0vl9</td><td> QVQLVQSGAEWKPGASVKISCKASGYTFTGYFMNWVKQSPGQSL EWIGRIHPYDGDTFYNQKFQGKATLTVDKSSNTAHMELLSLTSEDF AVYYCTRYDGSRAMDYWGQGTTVTVSSASTKGPSVYPLAPGSAAQ TNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLESDLYTLS SSVTVPSSMRPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPE VSSVFIFPPKPKDVLTITLTPKVTCVWDISKDDPEVQFSWFVDDVEV HTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAP lEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVE WQWNGQPAENYKNTQPIMNTNGSYFVYSKLNVQKSNWEAGNTFT CSVLHEGLHNHHTEKSLSHSPGK (SEQ ID NO:68)</td>

Table 6: Full-length light chain amino acid sequences

<td> Antibody</td><td> Full-length Light Chain Amino Acid Sequence (SEQ ID NO)</td>

<td> muFR!HC2-l (2.1)</td><td> SDVVLTQTPLSLPVNIGDQASISCKSSKSLLNSDGFTYLDWYLQKPG QSPQLLIYLVSNHFSGVPDRFSGSGSGTDFTLK1SRVEAEDLGVYYC FQSNYLPLTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASWCFL NNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTL TKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC (SEQ ID NO:34)</td>

<td> muFRlHC5-7 (5.7)</td><td> SDVVLTQTPLSLPVNIGDQASISCKSTESLLNSDGFTYLDWYLQKPG QSPQLL1YLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYC FQSNYLPLTFGGGTKLEVKRADAAPTVS1FPPSSEQLTSGGASVVCF LNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTL'T LTKDEYERHNSYTCEATHKTSTSP1VKSFNRNEC (SEQ ID NO:36)</td>

WO 2015/031815 &amp;#1470; 55 &amp;#1470;

<td> muFRIHC9-20 (9.20)</td><td> SDVVLTQTPLSLPVNLGDQASISCKSTKSLLNSDGFTYLDWYLQKP GQSPQLLIYLVSNHFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYY CFQSNYLPLTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCF LNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLT LTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC (SEQ ID NO:38)</td>

<td> muhuMovl9</td><td> DIVLTQSPLSLAVSLGQPAIISCKASQSVSFAGTSLMHWYHQKPGQQ PRLLIYRASNLEAGVPDRFSGSGSKTDFTLTISPVEAEDAATYYCQQ SREYPYTFGGGTKLEIKRTDAAPTVSIFPPSSEQLTSGGASWCFLNN FYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTK DEYERHNSYTCEATHKTSTSP1VKSFNRNEC (SEQ ID NO:69)</td>

[0213] Also provided are polypeptides that comprise: (a) a polypeptide having at least about 90% sequence identity to SEQ ID NOs:33, 35, or 37; and/or (b) a polypeptide having at least about 90% sequence identity to SEQ ID NOs:34, 36, or 38. In certain embodiments, the polypeptide comprises a polypeptide having at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% sequence identity to SEQ ID NOs:3338. Thus, in certain embodiments, the polypeptide comprises (a) a polypeptide having at least about 95% sequence identity to SEQ ID NOs:33, 35, or 37, and/or (b) a polypeptide having at least about 95% sequence identity to SEQ ID NOs:34, 36, or 38. In certain embodiments, the polypeptide comprises (a) a polypeptide having the amino acid sequence of SEQ ID NOs: 33, 35, or 37; and/or (b) a polypeptide having the amino acid sequence of SEQ ID NOs:34, 36, or 38. In certain embodiments, the polypeptide is an antibody and/or the polypeptide specifically binds FOLRI. In certain embodiments, the polypeptide is a murine, chimeric, or humanized antibody that specifically binds FOLRI. In certain embodiments, the polypeptide having a certain percentage of sequence identity to SEQ ID NOs:33-38 differs from SEQ ID NOs;33-38 by conservative amino acid substitutions only.

[0214] Also provided are polypeptides comprising a light chain that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical to the light chain sequence of the antibody produced by the hybridoma having ATCC deposit no. PTA 120196 or PTA-120197.

[0215] Also provided are polypeptides comprising a heavy chain that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical to the heavy chain sequence of the antibody produced by the hybridoma having ATCC deposit no. PTA120196 or PTA-120197.

&amp;#1470; -

[0216] Also provided are antibodies and antigen-binding fragments thereof comprising heavy and light chain sequences that are at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or identical to the heavy and light chain sequences of the antibody produced by the hybridoma having ATCC deposit no. PTA-120196 or PT A120197.

[0217] The affinity or avidity of an antibody for an antigen can be determined experimentally using any suitable method well known in the art, e.g., flow cytometry, enzyme-linked immunoabsorbent assay (ELISA), or radioimmunoassay (RIA), or kinetics (e.g., BIACORE™ analysis). Direct binding assays as well as competitive binding assay formats can be readily employed. (See, for example, Berzofsky, et al., Antibody-Antigen Interactions, In Fundamental Immunology, Paul, W. E., Ed., Raven Press: New York, N.Y. (1984); Kuby, Janis Immunology, W. H. Freeman and Company: New York, N.Y. (1992); and methods described herein. The measured affinity of a particular antibody-antigen, interaction can vary if measured under different conditions (e.g., salt concentration, pH, temperature). Thus, measurements of affinity and other antigen-binding parameters (e.g., KD or Kd, Kon, Koff) are made with standardized solutions of antibody and antigen, and a standardized buffer, as known in the art and such as the buffer described herein.

[0218[ In one aspect, binding assays can be performed using flow cytometry on cells expressing the FOLRI antigen on the surface. For example, FOLRI-positve cells such as SKOV3 can be incubated with varying concentrations of anti-FOLRl antibodies using 1 xlO5 cells per sample in 100 pL FACS buffer (RPMI-1640 medium supplemented with 2% normal goat serum). Then, the cells can be pelleted, washed, and incubated for 1 h with 100 pL of FITC-conjugated goat-anti-mouse or goat-anti-human IgG-antibody (such as is obtainable from, for example Jackson Laboratory, 6 pg/mL in FACS buffer). The cells are then pelleted again, washed with FACS buffer and resuspended in 200 pL of PBS containing 1% formaldehyde. Samples can be acquired, for example, using a FACSCalibur flow cytometer with the HTS multiwcll sampler and analyzed using CellQuest Pro (all from BD Bioscicnces, San Diego, US). For each sample the mean fluorescence intensity for FL1 (MFI) can be exported and plotted against the antibody concentration in a semi-log plot to generate a binding curve. A sigmoidal dose-response curve is fitted for binding curves and EC50 values are calculated using programs such as GraphPad Prism v4 with default parameters (GraphPad software, San Diego, CA). EC50 values can be used as a measure for the apparent dissociation constant Kd or KD for each antibody.

&amp;#1470; -

[0219] Monoclonal antibodies can be prepared using hybridoma methods, such as those described by Kohler and Milstein (1975) Nature 256:495. Using the hybridoma method, a mouse, hamster, or other appropriate host animal, is immunized to elicit the production by lymphocytes of antibodies that will specifically bind to an immunizing antigen. Lymphocytes can also be immunized in vitro. Following immunization, the lymphocytes are isolated and fused with a suitable myeloma cell line using, for example, polyethylene glycol, to form hybridoma cells that can then be selected away from unfused lymphocytes and myeloma cells. Hybridomas that produce monoclonal antibodies directed specifically against a chosen antigen as determined by immunoprecipitation, immunoblotting, or by an in vitro binding assay (e.g., radioimmunoassay (RIA); enzyme-linked immunosorbent assay (ELISA)) can then be propagated either in vitro culture using standard methods (Goding, Monoclonal Antibodies: Principles and Practice, Academic Press, 1986) or in vivo as ascites tumors in an animal. The monoclonal antibodies can then be purified from the culture medium or ascites fluid as described for polyclonal antibodies.

[0220] Alternatively monoclonal antibodies can also be made using recombinant DNA methods as described in U.S. Patent 4,816,567. The polynucleotides encoding a monoclonal antibody are isolated from mature B-cells or hybridoma cells, such as by RTPCR using oligonucleotide primers that specifically amplify the genes encoding the heavy and light chains of the antibody, and their sequence is determined using conventional procedures. The isolated polynucleotides encoding the heavy and light chains are then cloned into suitable expression vectors, which when transfected into host cells such as E. coli cells, simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein, monoclonal antibodies are generated by the host cells. Also, recombinant monoclonal antibodies or fragments thereof of the desired species can be isolated from phage display libraries expressing CDRs of the desired species as described (McCafferty et al., 1990, Nature, 348:552-554; Clackson et al., 1991, Nature, 352:624-628; and Marks et al., 1991, J. Mol. Biol., 222:581-597).

[0221] The polynucleotide(s) encoding a monoclonal antibody can further be modified in a number of different manners using recombinant DNA technology to generate alternative antibodies. In some embodiments, the constant domains of the light and heavy chains of, for example, a mouse monoclonal antibody can be substituted 1) for those regions of, for example, a human antibody to generate a chimeric antibody or 2) for a nonimmunoglobulin polypeptide to generate a fusion antibody. In some embodiments, the constant regions are truncated or removed to generate the desired antibody fragment of a

monoclonal antibody. Site-directed or high-density mutagenesis of the variable region can be used to optimize specificity, affinity, etc. of a monoclonal antibody.

[0222] In some embodiments, the monoclonal antibody against the human FOLRI is a humanized antibody. In certain embodiments, such antibodies are used therapeutically to reduce antigenicity and HAMA (human anti-mo use antibody) responses when administered to a human subject.

[0223] Methods for engineering, humanizing or resurfacing non-human or human antibodies can also be used and arc well known in the art. A humanized, resurfaced or similarly engineered antibody can have one or more amino acid residues from a source that is non-human, e.g., but not limited to, mouse, rat, rabbit, non-human primate or other mammal. These non-human amino acid residues are replaced by residues that are often referred to as import residues, which are typically taken from an import variable, constant or other domain of a known human sequence.

[0224] Such imported sequences can be used to reduce immunogenicity or reduce, enhance or modify binding, affinity, on-rate, off-rate, avidity, specificity, half-life, or any other suitable characteristic, as known in the art. In general, the CDR residues are directly and most substantially involved in influencing FOLRI binding. Accordingly, part or all of the non-human or human CDR sequences are maintained while the non-human sequences of the variable and constant regions can be replaced with human or other amino acids.

[0225] Antibodies can also optionally be humanized, resurfaced, engineered or human antibodies engineered with retention of high affinity for the antigen FOLRI and other favorable biological properties. To achieve this goal, humanized (or human) or engineered anti-FOLRl antibodies and resurfaced antibodies can be optionally prepared by a process of analysis of the parental sequences and various conceptual humanized and engineered products using three-dimensional models of the parental, engineered, and humanized sequences. Three-dimensional immunoglobulin models are commonly available and are familiar to those skilled in the art. Computer programs are available which illustrate and display probable three-dimensional conformational structures of selected candidate immunoglobulin sequences. Inspection of these displays permits analysis of the likely role of the residues in the functioning of the candidate immunoglobulin sequence, i.e., the analysis of residues that influence the ability of the candidate immunoglobulin to bind its antigen, such as FOLR1. In this way, framework (FR) residues can be selected and combined from the consensus and import sequences so that the desired antibody characteristic, such as increased affinity for the target antigen(s), is achieved.

&amp;#1470; -

[0226] Humanization, resurfacing or engineering of antibodies of the present invention can be performed using any known method, such as but not limited to those described in, Winter (Jones et al., Nature 321:522 (1986); Riechmann et al., Nature 332:323 (1988); Vcrhoeyen et al., Science 239:1534 (1988)), Sims et al., J. Immunol. 151: 2296 (1993); Chothia and Lesk, J. Mol. Biol. 196:901 (1987), Carter et al., Proc. Natl. Acad. Sci. U.S.A. 89:4285 (1992); Presta et al., J. Immunol. 151:2623 (1993), U.S. Pat. Nos. 5,639,641, 5,723,323; 5,976,862; 5,824,514; 5,817,483; 5,814,476; 5,763,192; 5,723,323; 5,766,886; 5,714,352; 6,204,023; 6,180,370; 5,693,762; 5,530,101; 5,585,089; 5,225,539; 4,816,567; PCT/: US98/16280; US96/18978; US91/09630; US91/05939; US94/01234; GB89/01334; GB91/01134; GB92/01755; WO90/14443; WO90/14424; WO90/14430; EP 229246; 7,557,189; 7,538,195; and 7,342,110, each of which is entirely incorporated herein by reference, including the references cited therein.

[0227] In certain alternative embodiments, the antibody to FOLRI is a human antibody. Human antibodies can be directly prepared using various techniques known in the art. Immortalized human B lymphocytes immunized in vitro or isolated from an immunized individual that produce an antibody directed against a target antigen can be generated (Sec, e.g., Cole et al., Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, p. 77 (1985); Boemer et al., 1991, J. Immunol., 147 (l):86-95; and U.S. Patent 5,750,373). Also, the human antibody can be selected from a phage library, where that phage library expresses human antibodies, as described, for example, in Vaughan et al., 1996, Nat. Biotech., 14:309314, Sheets et al., 1998, Proc, Nat’l. Acad. Sci,, 95:6157-6162, Hoogcnboom and Winter, 1991, J. Mol, Biol., 227:381, and Marks etal., 1991, J. Mol. Biol., 222:581). Techniques for the generation and use of antibody phage libraries are also described in U.S. Patent Nos. 5,969,108, 6,172,197, 5,885,793, 6,521,404; 6,544,731; 6,555,313; 6,582,915; 6,593,081; 6,300,064; 6,653,068; 6,706,484; and 7,264,963; and Rothe et al., 2007, J. Mol. Bio., doi:10.1016/j.jmb.2007.12.018 (each of which is incorporated by reference in its entirety). Affinity maturation strategies and chain shuffling strategies (Marks ct al., 1992, Bio/Technology 10:779-783, incorporated by reference in its entirety) are known in the art and can be employed to generate high affinity human antibodies.

[0228| Humanized antibodies can also be made in transgenic mice containing human immunoglobulin loci that arc capable upon immunization of producing the full repertoire of human antibodies in the absence of endogenous immunoglobulin production. This approach is desenbed in U.S. Patents 5,545,807; 5,545,806; 5,569,825; 5,625,126; 5,633,425; and 5,661,016.

&amp;#1470; -

[0229] In certain embodiments are provided an antibody fragment to, for example, increase tumor penetration. Various techniques are known for the production of antibody fragments. Traditionally, these fragments are derived via proteolytic digestion of intact antibodies (for example Morimoto et al., 1993, Journal of Biochemical and Biophysical Methods 24:107-117; Brennan et al., 1985, Science, 229:81). In certain embodiments, antibody fragments are produced recombinantly. Fab, Fv, and scFv antibody fragments can all be expressed in and secreted from E. coli or other host cells, thus allowing the production of large amounts of these fragments. Such antibody fragments can also be isolated from antibody phage libraries. The antibody fragment can also be linear antibodies as described in U.S. Patent 5,641,870, for example, and can be monospecific or bispecific. Other techniques for the production of antibody fragments will be apparent to the skilled practitioner.

[0230] For the purposes of the present invention, it should be appreciated that modified antibodies can comprise any type of variable region that provides for the association of the antibody with the polypeptides of a human FOLRI. In this regard, the variable region can comprise or be derived from any type of mammal that can be induced to mount a humoral response and generate immunoglobulins against the desired tumor associated antigen, As such, the variable region of the modified antibodies can be, for example, of human, murine, non-human primate (e.g., cynomolgus monkeys, macaques, etc.) or lupine origin. In some embodiments both the variable and constant regions of the modified immunoglobulins are human. In other embodiments the variable regions of compatible antibodies (usually derived from a non-human source) can be engineered or specifically tailored to improve the binding properties or reduce the immunogenicity of the molecule. In this respect, variable regions useful in the present invention can be humanized or otherwise altered through the inclusion of imported amino acid sequences.

[0231] In certain embodiments, the variable domains in both the heavy and light chains are altered by at least partial replacement of one or more CDRs and, if necessary, by partial framework region replacement and sequence changing. Although the CDRs can be derived from an antibody of the same class or even subclass as the antibody from which the framework regions are derived, it is envisaged that the CDRs will be derived from an antibody of different class and in certain embodiments from an antibody from a different species. It may not be necessary to replace all of the CDRs with the complete CDRs from the donor variable region to transfer the antigen-binding capacity of one variable domain to another. Rather, it may only be necessary to transfer those residues that arc necessary to maintain the activity of the antigen-binding site. Given the explanations set forth in U.S. Pat.

&amp;#1470; -

Nos. 5,585,089, 5,693,761 and 5,693,762, it will be well within the competence of those skilled in the art, either by carrying out routine experimentation or by trial and error testing to obtain a functional antibody with reduced immunogenicity.

[0232] Alterations to the variable region notwithstanding, those skilled in the art will appreciate that the modified antibodies of this invention will comprise antibodies (e.g., fulllength antibodies or immunoreactive fragments thereof) in which at least a fraction of one or more of the constant region domains has been deleted or otherwise altered so as to provide desired biochemical characteristics such as increased tumor localization or reduced serum half-life when compared with an antibody of approximately the same immunogenicity comprising a native or unaltered constant region. In some embodiments, the constant region of the modified antibodies will comprise a human constant region. Modifications to the constant region compatible with this invention comprise additions, deletions or substitutions of one or more amino acids in one or more domains. That is, the modified antibodies disclosed herein can comprise alterations or modifications to one or more of the three heavy chain constant domains (CHI, CH2 or CH3) and/or to the light chain constant domain (CL). In some embodiments, modified constant regions wherein one or more domains are partially or entirely deleted are contemplated. In some embodiments, the modified antibodies will comprise domain deleted constructs or variants wherein the entire CH2 domain has been removed (ACH2 constructs). In some embodiments, the omitted constant region domain will be replaced by a short amino acid spacer (e.g., 10 residues) that provides some of the molecular flexibility typically imparted by the absent constant region.

[0233] It will be noted that in certain embodiments, the modified antibodies can be engineered to fuse the CH3 domain directly to the hinge region of the respective modified antibodies. In other constructs it may be desirable to provide a peptide spacer between the hinge region and the modified CH2 and/or CH3 domains. For example, compatible constructs could be expressed wherein the CH2 domain has been deleted and the remaining CH3 domain (modified or unmodified) is joined to the hinge region with a 520&amp;#1470; amino acid spacer. Such a spacer can be added, for instance, to ensure that the regulatory elements of the constant domain remain free and accessible or that the hinge region remains flexible. However, it should be noted that amino acid spacers can, in some cases, prove to be immunogenic and elicit an unwanted immune response against the construct. Accordingly, in certain embodiments, any spacer added to the construct will be relatively non-immuno genic, or even omitted altogether, so as to maintai n the desired biochemical qualities of the modified antibodies.

WO 2015/031815 “ 62 -

[0234] Besides the deletion of whole constant region domains, it will be appreciated that the antibodies of the present invention car be provided by the partial deletion or substitution of a few or even a single amino acid. For example, the mutation of a single amino acid in selected areas of the CH2 domain may be enough to substantially reduce Fc binding and thereby increase tumor localization. Similarly, it may be desirable to simply delete that part of one or more constant region domains that control the effector function (e.g., complement C1Q binding) to be modulated. Such partial deletions of the constant regions can improve selected characteristics of the antibody (serum half-life) while leaving other desirable functions associated with the subject constant region domain intact. Moreover, as alluded to above, the constant regions of the disclosed antibodies can be modified through the mutation or substitution of one or more amino acids that enhances the profile of the resulting construct. In this respect it may be possible to disrupt the activity provided by a conserved binding site (e.g., Fc binding) while substantially maintaining the configuration and immunogenic profile of the modified antibody. Certain embodiments can comprise the addition of one or more amino acids to the constant region to enhance desirable characteristics such as decreasing or increasing effector function or provide for more cytotoxin or carbohydrate attachment. In such embodiments it can be desirable to insert or replicate specific sequences derived from selected constant region domains.

[0235] The present invention further embraces variants and equivalents which are substantially homologous to the chimeric, humanized and human antibodies, or antibody fragments thereof, set forth herein. These can contain, for example, conservative substitution mutations, i.e., the substitution of one or more amino acids by similar amino acids. For example, conservative substitution refers to the substitution of an amino acid with another within the same general class such as, for example, one acidic amino acid with another acidic amino acid, one basic amino acid with another basic amino acid or one neutral amino acid by another neutral amino acid. What is intended by a conservative amino acid substitution is well known in the art.

[0236] The polypeptides of the present invention can be recombinant polypeptides, natural polypeptides, or synthetic polypeptides comprising an antibody, or fragment thereof, against a human FOLRI. It will be recognized in the art that some amino acid sequences of the invention can be varied without significant effect of the structure or function of the protein. Thus, the invention further includes variations of the polypeptides which show substantial activity or which include regions of an antibody, or fragment thereof, against a

&amp;#1470; - human folate receptor protein. Such mutants include deletions, insertions, inversions, repeats, and type substitutions.

[0237] The polypeptides and analogs can be further modified to contain additional chemical moieties not normally part of the protein. Those derivatized moieties can improve the solubility, the biological half life or absorption of the protein. The moieties can also reduce or eliminate any desirable side effects of the proteins and the like. An overview for those moieties can be found in REMINGTON'S PHARMACEUTICAL SCIENCES, 20th ed., Mack Publishing Co., Easton, PA (2000).

[0238] The isolated polypeptides described herein can be produced by any suitable method known in the art. Such methods range from direct protein synthetic methods to constructing a DNA sequence encoding isolated polypeptide sequences and expressing those sequences in a suitable transformed host. In some embodiments, a DNA sequence is constructed using recombinant technology by isolating or synthesizing a DNA sequence encoding a wild-type protein of interest. Optionally, the sequence can be mutagenized by site-specific mutagenesis to provide functional analogs thereof. See, e.g., Zoeller et al., Proc. Nat’l. Acad. Sci. USA 81:5662-5066 (1984) and U.S. Pat. No. 4,588,585.

[0239] In some embodiments a DNA sequence encoding a polypeptide of interest would be constructed by chemical synthesis using an oligonucleotide synthesizer. Such oligonucleotides can be designed based on the amino acid sequence of the desired polypeptide and selecting those codons that are favored in the host cell in which the recombinant polypeptide of interest will be produced. Standard methods can be applied to synthesize an isolated polynucleotide sequence encoding an isolated polypeptide of interest. For example, a complete amino acid sequence can be used to construct a back-translated gene. Further, a DNA oligomer containing a nucleotide sequence coding for the particular isolated polypeptide can be synthesized. For example, several small oligonucleotides coding for portions of the desired polypeptide can be synthesized and then ligated. The individual oligonucleotides typically contain 5' or 3' overhangs for complementary assembly.

[0240] Once assembled (by synthesis, site-directed mutagenesis or another method), the polynucleotide sequences encoding a particular isolated polypeptide of interest will be inserted into an expression vector and operatively linked to an expression control sequence appropriate for expression of the protein in a desired host. Proper assembly can be confirmed by nucleotide sequencing, restriction mapping, and expression of a biologically active polypeptide in a suitable host. As is well known in the art, in order to obtain high expression levels of a transfected gene in a host, the gene must be operatively linked to transcriptional

WO 2015/031815 &amp;#1470; 64 &amp;#1470; and translational expression control sequences that are functional in the chosen expression host.

[0241] In certain embodiments, recombinant expression vectors axe used to amplify and express DNA encoding antibodies, or fragments thereof, against human FOLRI. Recombinant expression vectors are replicable DNA constructs which have synthetic or cDNA-derived DNA fragments encoding a polypeptide chain of an anti-FOLRl antibody, or fragment thereof, operatively linked to suitable transcriptional or translational regulatory elements derived from mammalian, microbial, viral or insect genes. A transcriptional unit generally comprises an assembly of (1) a genetic element or elements having a regulatory role in gene expression, for example, transcriptional promoters or enhancers, (2) a structural or coding sequence which is transcribed into mRNA and translated into protein, and (3) appropriate transcription and translation initiation and termination sequences. Such regulatory elements can include an operator sequence to control transcription. The ability to replicate in a host, usually conferred by an origin of replication, and a selection gene to facilitate recognition of transformants can additionally be incorporated. DNA regions are operatively linked when they are functionally related to each other. For example, DNA for a signal peptide (secretory leader) is operatively linked to DNA for a polypeptide if it is expressed as a precursor which participates in the secretion of the polypeptide; a promoter is operatively linked to a coding sequence if it controls the transcription of the sequence; or a ribosome binding site is operatively linked to a coding sequence if it is positioned so as to permit translation. Structural elements intended for use in yeast expression systems include a leader sequence enabling extracellular secretion of translated protein by a host cell. Alternatively, where recombinant protein is expressed without a leader or transport sequence, it can include an N-terminal methionine residue. This residue can optionally be subsequently cleaved from the expressed recombinant protein to provide a final product.

[0242] The choice of expression control sequence and expression vector will depend upon the choice of host. A wide variety of expression host/vector combinations can be employed. Useful expression vectors for eukaryotic hosts, include, for example, vectors comprising expression control sequences from. SV40, bovine papilloma virus, adenovirus and cytomegalovirus. Useful expression vectors for bacterial hosts include known bacterial plasmids, such as plasmids from Escherichia coli, including pCR 1, pBR322, pMB9 and their derivatives, wider host range plasmids, such as M13 and fdamentous single-stranded DNA phages.

&amp;#1470; -

[0243] Suitable host cells for expression of a FOLRI -binding polypeptide or antibody (or a FOLRI protein to use as an antigen) include prokaryotes, yeast, insect or higher eukaryotic cells under the control of appropriate promoters. Prokaryotes include gram negative or gram positive organisms, for example E. coli or bacilli. Higher eukaryotic cells include established cell lines of mammalian origin. Cell-free translation systems could also be employed. Appropriate cloning and expression vectors for use with bacterial, fungal, yeast, and mammalian cellular hosts are described by Pouwels et al. (Cloning Vectors: A Laboratory Manual, Elsevier, N.Y., 1985), the relevant disclosure of which is hereby incorporated by reference. Additional information regarding methods of protein production, including antibody production, can be found, e.g., in U.S. Patent Publication Mo. 2008/0187954, U.S. Patent Nos. 6,413,746 and 6,660,501, and International Patent Publication No. WO 04009823, each of which is hereby incorporated by reference herein in its entirety.

[0244] Various mammalian or insect cell culture systems are also advantageously employed to express recombinant protein. Expression of recombinant proteins in mammalian cells can be performed because such proteins are generally correctly folded, appropriately modified and completely functional. Examples of suitable mammalian host cell lines include HEK-293 and HEK-293T, the COS-7 lines of monkey kidney cells, described by Gluzman (Cell 23:175, 1981), and other cell lines including, for example, L cells, C127, 3T3, Chinese hamster ovary (CHO), HeLa and BHK cell lines. Mammalian expression vectors can comprise nontranscribed elements such as an origin of replication, a suitable promoter and enhancer linked to the gene to be expressed, and other 5' or 3' flanking nontranscribed sequences, and 5’ or 3' nontranslated sequences, such as necessary ribosome binding sites, a polyadenylation site, splice donor and acceptor sites, and transcriptional termination sequences. Baculovirus systems for production of heterologous proteins in insect cells are reviewed by Luckow and Summers, Bio/Technology 6:47 (1988).

[0245] The proteins produced by a transformed host can be purified according to any suitable method. Such standard methods include chromatography (e.g., ion exchange, affinity and sizing column chromatography), centrifugation, differential solubility, or by any other standard technique for protein purification. Affinity tags such as hexahistidine, maltose binding domain, influenza coat sequence and glutathione-S-transferase can be attached to the protein to allow easy purification by passage over an appropriate affinity column. Isolated proteins can also be physically characterized using such, techniques as proteolysis, nuclear magnetic resonance and x-ray crystallography.

WO 2015/031815 &amp;#1470; 66 &amp;#1470;

[0246] For example, supernatants from systems which secrete recombinant protein into culture media can be first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration unit. Following the concentration step, the concentrate can be applied to a suitable purification matrix. Alternatively, an anion exchange resin can be employed, for example, a matrix or substrate having pendant diethylaminoethyl (DEAE) groups. The matrices can be acrylamide, agarose, dextran, cellulose or other types commonly employed in protein purification. Alternatively, a cation exchange step can be employed. Suitable cation exchangers include various insoluble matrices comprising sulfopropyl or carboxymethyl groups. Finally, one or more reversed-phase high performance liquid chromatography (RPHPLC) steps employing hydrophobic RP-HPLC media, e.g., silica gel having pendant methyl or other aliphatic groups, can be employed to further purify a FOLRl-binding agent. Some or all of the foregoing purification steps, in various combinations, can also be employed to provide a homogeneous recombinant protein.

[0247] Recombinant protein produced in bacterial culture can be isolated, for example, by initial extraction from cell pellets, followed by one or more concentration, salting-out, aqueous ion exchange or size exclusion chromatography steps. High performance liquid chromatography (HPLC) can be employed for final purification steps. Microbial cells employed in expression of a recombinant protein can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or use of cell lysing agents. [0248] Methods known in the art for purifying antibodies and other proteins also include, for example, those described in U.S. Patent Publication Nos. 2008/0312425, 2008/0177048, and 2009/0187005, each of which is hereby incorporated by reference herein in its entirety.

III. Polynucleotides

[02491 In certain embodiments, the invention encompasses polynucleotides comprising polynucleotides that encode a polypeptide that specifically binds a human FOLRI receptor or a fragment of such a polypeptide. For example, the invention provides a polynucleotide comprising a nucleic acid sequence that encodes an antibody to a human

FOLRI or encodes a fragment of such an antibody. Ihe polynucleotides of the invention can be in the form of RNA or in the form of DNA. DNA includes cDNA, genomic DNA, and synthetic DNA; and can be double-stranded or single-stranded, and if single stranded can be

&amp;#1470; - the coding strand or non-coding (anti-sense) strand. Tn some embodiments, the polynucleotide is a cDNA or a DNA lacking one more endogenous introns.

[0250] In some embodiments, a polynucleotide is a non-naturally occurring polynucleotide. In some embodiments, a polynucleotide is recombinantly produced.

[0251] In certain embodiments, the polynucleotides are isolated. In certain embodiments, the polynucleotides are substantially pure. In some embodiments, a polynucleotide is purified from natural components.

[0252] The invention provides a polynucleotide comprising a polynucleotide encoding a polypeptide comprising a sequence selected from the group consisting of SEQ ID NOs:3-38 and 59-67. Also provided is a polynucleotide encoding a polypeptide having at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% sequence identity to SEQ ID NOs:3-38 and 59-67.

[0253] The invention further provides a polynucleotide comprising a sequence selected from those shown in Tables 7 and 8 below.

Table 7: Variable heavy chain polynucleotide sequences

<td> Antibody</td><td> Variable Heavy Chain Polynucleotide Sequence (SEQ ID NO)</td>

<td> muFRIHC2-l (2.1)</td><td> caggtccaactgcagcagtctggacctgagctggtgaagcctggggcttcagtgaggatatcctgcaagg cttctggctacaccttcacaaactcctatattcactgggtgaaaaagaggcctggacagggacttgagtgga ttggatggatttatcctgaaagtcttaatactcaatacaatgagaagttcaaggccaaggccacactgactgct gacaagtcctccagcacatcctacatgcagctcagcagtctgacctctgaggactctgcggtctatttctgtg caagaaggggtatttattactactctccctatgctctggaccactggggtcaaggagcctcagtcaccgtctc ctca(SEQ ID NO:39)</td>

<td> muFRIHC5-7 (5.7)</td><td> caggtccaactgcagcagtctggacctgaggtggtgaagcctggggcttcagtgaggatatcctgcaagg cttctggctacaccttcacaaactactatatacactgggtgaagcagaggcctggacagggacttgagtgga ttggatggatttatcctggaagttttaatgttgagtacaatgagaagttcaaggccaaggccacactgactgc agacaaatcctcc agcacagtctacatgcaactc age agcctgacc tctgaggactctgcg gtctattt etgt gcaagaaggggtatttatttctactctccctatgctttggactactggggtcaaggagcctcagtcaccgtctc ctca (SEQ ID NO:41)</td>

<td> muFR!HC9-20 (9.20)</td><td> caggtccaactgcagcagtctggacctgacctggtgaagcctggggcttcagtgaggatatcctgcaagg ettet ggc ttc accttc ac aaactactatatacactgggtgaa gcagaggcctggacagggacttga gtgga ttggatggatttatcctgaaaatgttaatgttaggtacaatgacaagttcaaggccaaggccacactgactgc agacaaatcctccagcacagcctacatgcagctcagcagcctgacctctgaggactctgcggtctatttctg tgcaagaaggggtatttattactactctccctatgctatggactactggggtcaaggagcctcagtcaccgtct cctca (SEQ ID NO:43)</td>

<td> huFRIHC2-l (resurfaced)</td><td> aagcttgccaccATGGGTTGGAGCTGCATTATCCTTTTCCTTGTGGCTA CAGCTACTGGCGTTCACTCTCAGGTACAATTGGTTCAGTCAGGA GCCGAGGTCGTAAAGCCCGGTGCCAGTGTGAAGATCTCATGCAA GGCAAGCGGTTATACTTTTACAAACTCTTACATTCATTGGGTGAA AAAGCGGCCCGGCCAGGGTCTCGAATGGATCGGCTGGATCTACC CAGAAAGTCTGAACACTCAATACAACCAGAAGTTTCAGGGTAAG GCAACTCTCACTGCCGACAAGAGCTCTAGCACAAGCTATATGCA GTTGTCTAGTTTGACAAGCGAGGATAGCGCAGTTTACTTTTGTGC TCGGCGTGGTATTTATTACTACTCACCTTATGCTCTGGATCACTG GGGACAGGGTGCCTCTGTTACCGTTTCCAGTGCATCCACCaagggcc c (SEQ ID NO :70)</td>

<td> huFRIHC2-l (grafted)</td><td> aagcttgccaccATGGGCTGGAGCTGCATAATCCTCTTCCTCGTAGC TACCGCCACTGGGGTGCATTCTCAAGTACAGTTGGTGCAGTC CGGAGCTGAAGTCAAGAAGCCAGGGGCTTCTGTTAAGGTGA GCTGTAAGGCTTCCGGATATACCTTCACAAACAGTTATATCC ATTGGGTGAGGCAAGCTCCAGGCCAGGGTCTCGAATGGATG GGATGGATCTACCCCGAGAGTCTGAACACCCAGTACAACGA GAAGTTCAAGGCACGTGTGACCATGACAAGAGACACCTCCA TCAGTACAGCCTATATGGAATTGAGCCGTCTCAGAAGTGATG ATACAGCAGTGTACTACTGCGCCAGGCGGGGCATCTACTACT ACAGCCCATACGCTCTCGACCACTGGGGACAAGGAACACTG GTAACCGTAAGCTCAGCTTCTACAaagggccc (SEQ ID NO:71)</td>

Table 8: Variable light chain polynucleotide sequences

<td> Antibody</td><td> Variable Light Chain Polynucleotide Sequence (SEQ ID NO)</td>

<td> muFRIHC2-l (2.1)</td><td> agtgatgttgttctgacccaaactccactctctctgcctgtcaatattggagatcaagcctctatctcttgcaagt cttctaagagtcttctgaatagtgatggattcacttatttggactggtacctgcagaagccaggccagtctcca cagctcctaatatatttggtttctaatcatttttctggagttccagacaggttcagtggcagtgggtcaggaaca gatttcacactcaagatcagcagagtggaggctgaggatttgggagtttattattgcttccagagtaactatctt cctctcacgttcggaggggggaccaagctggaaataaaacgg (SEQ ID NO:40)</td>

<td> muFRIHC5-7 (5.7)</td><td> agtgatgttgttctgacccaaactccactctctctgcctgtcaatattggagatcaagcctctatctcttgcaagt ctactgagagtcttctgaatagtgatggattcacttatttggactggtacctgcagaagccaggccagtctcca c agctcctaat at attt ggtttctaatcatttttctggagttc c agacaggttc agtggcagtgggtcaggaaca gatttcacactcaagatcagcagagtggaggctgaggatttgggagtttattattgcttccagagtaactatctt cctctcacgttcggaggggggaccaagctggaagtaaaacgg (SEQ ID NO:42)</td>

<td> muFRIHC9-20 (9.20)</td><td> agtgatgttgttctgacccaaactccactctctctgcctgtcaatcttggagatcaagcctctatctcttgcaagt ctactaagagtcttctgaatagtgatggattcacttatttggactggtacctgcagaagccaggccagtctcca cagctcctaatatatttggtttctaatcatttttctggagttccagacaggttcagtggcagtgggtcaggaaca gatttcaccctcaagatcagcagagtggaggctgaggatttgggagtttattattgcttccagagtaactatctt cctctcacgttcggaggggggaccaagctggaaataaaacgg (SEQ ID NO:44)</td>

&amp;#1470; -

<td> huFRIHC2-l v. 1.0 (resurfaced)</td><td> gaattcgccaccATGGGTTGGTCATGTATAATACTTTTCCTGGTAGC TACTGCTACTGGTGTGCATTCAGATGTGGTGCTGACTCAGTC ACCCTTGTCTCTCCCAGTCAATCTTGGGCAGCCAGCATCTATC AGCTGCCGAAGCAGCAGGTCTCTCCTGAACTCCGATGGCTTT ACTTATCTTGACTGGTATCTCCAGAAGCCAGGACAGTCCCCC CGGCTGCTCATCTACCTGGTTTCTAATCATTTTAGTGGCGTCC CTGACCGCTTCTCTGGGAGTGGAAGTGGGACCGATTTTACAC TGAAGA.TCTCCAGGGTCGAAGCTGAGGACCTTGGGGTTTACT ACTGTTTCCAGAGCAACTACCTTCCCTTGACATTCGGCCAGG GAACCAAGCTGGAAATCAAGcgtacg (SEQ ID NO:72)</td>

<td> huFRIHC2-l v. 1.01 (resurfaced)</td><td> gaattcgccaccATGGGTTGGTCTTGTATCATTCTGTTCCTGGTCGC CACTGCCACAGGAGTTCACTCAGACGTGGTACTCACACAATC TCCCCTTTCCCTGCCTGTGAACCTGGGACAGCCAGCCTCAAT CAGTTGCAAGAGCTCTAAATCTCTGCTCAATAGCGATGGCTT TACCTACTTGGATTGGTACCTCCAGAAGCCCGGCCAGTCTCC TCGGCTCCTGATTTACCTTGTTTCAAATCACTTTTCAGGCGTG CCTGACCGGTTCTCCGGATCTGGCTCAGGGACAGACTTCACC CTGAAGATCTCCCGCGTCGAGGCAGAGGATCTCGGCGTGTAT TACTGTTTCCAAAGTAACTACCTGCCATTGACTTTTGGACAA GGAACTAAACTGGAAATCAAAcgtacg (SEQ ID NO:73)</td>

<td> huFRIHC2-l v. 1.0 (grafted)</td><td> gaattcgccaccATGGGATGGAGTTGTATTATTCTGTTCTTGGTCGC TACTGCAACAGGCGTTCATTCTGACATCGTAATGACCCAGAC ACCTCTGAGTCTGAGTGTCACTCCCGGCCAGCCCGCCTCTATT TCATGTCGTAGCTCTCGCTCCCTGCTCAATTCCGACGGTTTTA CCTACTTGGACTGGTATCTTCAGAAACCTGGGCAGAGCCCTC AGCTTCTGATCTATCTGGTGTCCAATCACTTCAGTGGCGTCCC AGACCGATTTTCCGGAAGCGGAAGCGGAACCGACTTTACCCT GAAGATATCCCGCGTCGAAGCAGAGGACGTGGGCGTGTATT ATTGCTTTCAAAGCAATTACTTGCCATTGACTTTCGGACAAG GCACAAAACTGGAGATTAAGcgtacg (SEQ ID NO:74)</td>

<td> huFRIHC2-l v. 1.01 (grafted)</td><td> gaattcgccaccATGGGCTGGTCATGCATCATACTGTTCCTGGTGGC TACAGCAACCGGGGTGCACAGCGATATTGTTATGACACAGAC ACCACTGAGTTTGTCAGTGACCCCCGGCCAGCCAGCCTCTAT ATCCTGCAAGTCCTCAAAAAGTCTCCTGAATAGCGATGGCTT TACCTACCTCGACTGGTATCTTCAGAAGCCCGGTCAAAGCCC TCAGCTGCTGATATATCTGGTGTCTAACCATTTTAGCGGAGTC CCCGACCGCTTTTCAGGCTCCGGCAGTGGCACCGACTTCACC CTTAAGATTTCTCGCGTGGAGGCTGAAGATGTAGGGGTCTAC TACTGTTTCCAGTCAAACTACCTGCCACTGACCTTTGGTCAAG GCACTAAGCTCGAAATTAAGcgtacg (SEQ ID NO:75)</td>

[0254| Also provided is a polynucleotide having at least about 95%, at least about

96%, at least about 97%, at least about 98%, or at least about 99% sequence identity to any one of SEQ ID NOs:39-44.

[0255] Also provided arc polynucleotides encoding a variable light chain that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical

&amp;#1470; to the variable light chain sequence of the antibody produced by the hybridoma having ATCC deposit no. PTA-120196 or PTA-120197.

[0256] Also provided are polynucleotides comprising a variable light chain-encoding sequence that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical to the variable light chain-encoding sequence that encodes the variable light chain of the antibody produced by the hybridoma having ATCC deposit no. PTA120196 or PT A-120197.

[0257] Also provided are polynucleotides encoding a variable heavy chain that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical to the variable heavy chain sequence of the antibody produced by the hybridoma having ATCC deposit no. PTA-120196 or PTA-120197. '

[0258] Also provided are polynucleotides comprising a variable heavy chainencoding sequence that is at least about 85%, at least about 90%, at least about 95%, or at least about 99%, or is identical to the variable heavy chain-encoding sequence that encodes the variable heavy chain of the antibody produced by the hybridoma having ATCC deposit no. PTA-120196 or PTA-120197.

[0259] In certain embodiments the polynucleotides comprise the coding sequence for the mature polypeptide fused in the same reading frame to a polynucleotide which aids, for example, in expression and secretion of a polypeptide from a host cell (e.g., a leader sequence which functions as a secretory sequence for controlling transport of a polypeptide from the cell). The polypeptide having a leader sequence is a preprotein and can have the leader sequence cleaved by the host cell to form the mature form of the polypeptide. The polynucleotides can also encode for a proprotein which is the mature protein plus additional 5' amino acid residues. A mature protein having a prosequence is a proprotein and is an inactive form of the protein. Once the prosequcnce is cleaved an active mature protein remains.

[0260] In certain embodiments the polynucleotides comprise the coding sequence for the mature polypeptide fused in the same reading frame to a marker sequence that allows, for example, for purification of the encoded polypeptide. For example, the marker sequence can be a hexa-histidinc tag supplied by a pQE-9 vector to provide for purification of the mature polypeptide fused to the marker in the case of a bacterial host, or the marker sequence can be a hemagglutinin (HA) tag derived from the influenza hemagglutinin protein when a mammalian host (e.g., COS-7 cells) is used.

WO 2015/031815 &amp;#1470; 71 &amp;#1470;

[0261] The present invention further relates to variants of the hereinabove described polynucleotides encoding, for example, fragments, analogs, and derivatives.

[0262] The polynucleotide variants can contain, alterations in the coding regions, non&amp;#1524; coding regions, or both. In some embodiments the polynucleotide variants contain alterations which produce silent substitutions, additions, or deletions, but do not alter the properties or activities of the encoded polypeptide. In some embodiments, nucleotide variants are produced by silent substitutions due to the degeneracy of the genetic code. Polynucleotide variants can be produced for a variety of reasons, e.g., to optimize codon, expression for a particular host (change codons in the human mRNA to those preferred by a bacterial host such as E. coll).

[0263] Vectors and cells comprising the polynucleotides described herein are also provided.

IV. Biological samples

[0264] Biological samples are often fixed with a fixative. Aldehyde fixatives such as formalin (formaldehyde) and glutaraldehyde are typically used. Tissue samples fixed using other fixation techniques such as alcohol immersion (Battifora and Kopinski, J. Histochem. Cytochem. (1986) 34:1095) are also suitable. The samples used may also be embedded in paraffin. In one embodiment, the samples are both formalin-fixed and paraffin-embedded (FFPE). In another embodiment, the FFPE block is hematoxylin and eosin stained prior to selecting one or more portions for analysis in order to select specific area(s) for the FFPE core sample. Methods of preparing tissue blocks from, these particulate specimens have been used in previous IHC studies of various prognostic factors, and/or is well known to those of skill in the art (see, for example, Abbondanzo et al., Am J Clin Pathol. 1990 May;93(5):698702; Allred et al., Arch Surg. 1990 Jan;125(l):107-13).

[0265] Briefly, any intact organ or tissue may be cut into fairly small pieces and incubated in various fixatives (e.g. formalin, alcohol, etc.) for varying periods of time until the tissue is fixed. The samples may be virtually any intact tissue surgically removed from the body. The samples may be cut into reasonably small piece(s) that fit on the equipment routinely used in histopathology laboratories. The size of the cut pieces typically ranges from a few millimeters to a few centimeters. The biological sample can also be fluidic extracts, blood, plasma, serum, spinal fluid, lymph fluid, and or splenic preparations.

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V. Correlation, of FOLRI expression and therapeutic efficacy

[0266] The antibody maytansinoid conjugate (AMC) IMGN853 comprises the FOLRI-binding monoclonal antibody, huMovl9 (M9346A), conjugated to the maytansinoid, DM4 (N(2')-deacetyl-N2'4)&amp;#1470;-mercapto methyl-l-oxopentyl)-maytansine), attached via the cleavable sulfo-SPDB (N-succinimidyl 4-(2-pyridyl dithio) sulfobutanoate) linker. The antibody sequences of IMGN853 (huMovl9) are provided below as SEQ ID NOs: 45 and 47, and IMGN853 and huM0v!9 are described in US Appl. Pub. No. 2012/0009181 (now 8,557,966), which is herein incorporated by reference in its entirety.

SEQ ID NO:45 - huM0vl9 vHC

QVQLVQSGAEWKPGASVKISCKASGYTFTGYFMNWVKQSPGQSLEW1GRIHPYDG DTFYNQKFQGKATLTVDKSSNTAHMELLSLTSEDFAVYYCTRYDGSRAMDYWGQG TTVTVSS

SEQ ID NO:46 - huM0vl9 vLCvl .00

DIVLTQSPLSLAVSLGQPAIISCKASQSVSFAGTSLMHWYHQKPGQQPRLLIYRASNL EAGVPDRFSGSGSKTDFTLNISPVEAEDAATYYCQQSREYPYTFGGGTKLEIKR SEQ ID NO:47 - huM0v!9 vLCvl .60

DIVLTQSPLSLAVSLGQPAI1SCKASQSVSFAGTSLMHWYHQKPGQQPRLLIYRASNL EAGVPDRFSGSGSKTDFTLTISPVEAEDAATYYCQQSREYPYTFGGGTKLEIKR SEQ ID NO:48 - huM0vl9 vLC CDRl KASQSVSFAGTSLMH

SEQ ID NO:49 - huM0vl9 vLC CDR2

RASNLEA

SEQ ID NO:50 - huM0v!9 vLC CDR3

QQSREYPYT

SEQ ID NO:51 - huMovl 9 vHC CDR 1

GYFMN

SEQ ID NO:52 - huM0vl9 vHC CDR2 - Kabat Defined

R1HPYDGDTFYNQKFQG

SEQ ID NO:53 - huMovl 9 vHC CDR2 - Abm Defined RIHPYDGDTF

SEQ ID NO:54 - huM0vl9 vHC CDR3 YDGSRAMDY

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SEQ ID NO:55 - huM0vl9 HC amino acid sequence

QVQLVQSGAEWKPGASVKISCKASGYTFTGYFMNWVKQSPGQSLEWIGRIHPYDG DTFYNQKFQGKATLTVDKSSNTAHMELLSLTSEDFAVYYCTRYDGSRAMDYWGQG TTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVH TFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTC PPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDPEVKFNWY\/DGVE VHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA KGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPP VLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

SEQ ID NO:56 - huM0v!9 LCvl.OO

DIVLTQSPLSLAVSLGQPAIISCKASQSVSFAGTSLMHWYHQKPGQQPRLLIYRASNL EAGVPDRFSGSGSKTDFTLN1SPVEAEDAATYYCQQSREYPYTFGGGTKLEIKRTVAA PSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSK DSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

SEQ ID NO:57 - huM0vl9 LCvl.60

DIVLTQSPLSLAVSLGQPAIISCKASQSVSFAGTSLMHWYHQKPGQQPRLLIYRASNL EAGVPDRFSGSGSKTDFTLTISPVEAEDAATYYCQQSREYPYTFGGGTKLEIKRTVAA PSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSK DSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

SEQ ID NO;58 - muM0v!9 vHC CDR2 - Kabat Defined

RIHPYDGDTFYNQNFKD

[0267] IMGN853 is currently in clinical development for various therapeutic indications which include FOLRI positive ovarian cancer, non-small cell lung cancer, endometrioid cancer, renal cancer, and other epithelial malignancies. Ovarian cancers exhibit the greatest FOLRI penetrance and are considered the major indications for treatment with IMGN853 (Antony AC. Arm Rev Nutr 16:501-21 (1996); Yuan Y et al. Hum Pathol 40(10):1453-1460 (2009)). Measuring levels of FOLRI in patient plasma samples can help identify patient populations more likely to respond to AM.C treatment.

[0268] Tn certain embodiments, the invention provides a method for identifying subjects that arc likely to respond favorably to FOLRI-targeting anti-cancer therapies due to elevated expression levels of FOLRI being expressed in the subject, in particular using antibodies and antigen-binding fragments thereof provided herein that can detect a dynamic range of FOLRI expression levels, e.g., in 1HC.

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[0269] Evaluation of patient samples and correlation to in vivo efficacy using xenograft models demonstrates the power of the expression analysis for selecting subjects more likely to respond to treatment. IHC provides a score for FOLRI expression on tumor cells: 0 (no expression) to 3 (or 3+) (very high levels of expression). In vivo data using xenograft models demonstrates that samples scoring 2. or 3 (or 3+) for FOLRI expression have an increased likelihood to respond to FOLRI -targeted anti-cancer therapies at clinicallyrelevant doses of FOLRI immunoconjugates (see e.g., U.S. Provisional Application Nos. 61/823,317 and 61/828,586 and International Application No. all of which are herein incorporated by reference in their entireties). Thus, identification of individuals having an elevated FOLR1 score would help identify those individuals who might respond to a clinically relevant dosage. Moreover, expression of more uniform levels of FOLRI provides better correlation with therapeutic benefit. Thus, a homogenous staining uniformity or a combination of increased staining with heterogenous staining uniformity can indicate increased FOLRI expression. For example, scores of greater than 2 hetero may be used as a patient selection criterion for treatment with a FOLRI therapeutic agent (see e.g., U.S. Published Application No. 2012/0282175, which is herein incorporated by reference in its entirety).

[0270] FOLRI expression analysis also identifies patients in whom decreased levels of a FOLRI-targeting anti-cancer therapy (“low dose therapy”) can be effective to cause antitumor responses. As is appreciated in the art, compounds are generally administered at the smallest dosage that achieves the desired therapeutic response. This is specifically important for therapeutics that cause clinical side effects. The ability to recognize those subjects with elevated FOLRI expression levels allows for minimization of the dosage of the FOLRItargeting therapeutic, thus decreasing possible side effects, while maintaining therapeutic efficacy.

[0271] Accordingly, the antibodies and antigen-binding fragments provided herein are particularly advantageous for use in such methods because they are capable of detecting a dynamic range of FOLRI expression levels, e.g., in IHC.

VI. Shed antigen assay

[0272] Measuring levels of circulating antigen in patient plasma samples (shed antigen) can help identify patient populations more likely to respond to treatment, e.g., antibody maytansinoid conjugate (AMC) treatment. High levels of shed antigen have been reported to markedly affect the pharmacokinetics of therapeutic antibodies (Tolchcr A. et al.

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20th Symposium on Molecular Targets and Cancer Therapeutics; October 21-24, 2008; Geneva, Switzerland: EORTC-NCI-AACR, pl63, #514; Baselga J, et al. J Clin Oncol 14:737-744 (1996)). It is likely that shed antigen levels from patient plasma samples will be variable depending on factors such as antigen target, disease indications, and disease course. Currently shed antigen levels in disease indications for the anti-FOLRl immunoconjugate IMGN853 have been insufficiently examined while correlation with solid tumor expression is limited. While elevation of FOLRI has been reported in ovarian adenocarcinomas, data suggests that it is not elevated in other FOLRI + tumor indications, such, as small cell lung carcinoma (Mantovani LT, et al. Fur J Cancer 30A(3):363-9 (1994); Basal E, et al. PLoS ONE 4(7): e6292 (2009)). The present method allows for detection of the FOLRI receptor in the presence of high folic acid using the antibodies and antigen-binding fragments thereof that are provided herein and are capable of detecting dynamic ranges of shed FOLRI. Previous assays have used Movl9 in the design of the assay. Since IMGN853 contains Movl9 and in one embodiment is the targeted therapy of the invention, it is vital that the method detects FOLRI in the presence or absence of Movl9. Previous assays that use Movl9 have competitive effects and will detect significantly less or no FOLRI in patients receiving IMGN853 treatment.

[0273] In one embodiment, the present method for detecting FOLRI in human sourced fluid samples uses a traditional sandwich ELISA format. In one embodiment, the method uses a capture agent (i.e., antibody) to FOLRI attached to a solid support. In one embodiment, the solid support is a microtiter plate. To this, the sample (ascites fluids, plasma, etc.) is added without dilution, and is detected by a different detection agent (a different antibody), &amp;#1470;which does not interfere with the binding of the first capture agent. The detection agent is then detected through the use of a secondary detection agent (biotin / streptavidin, anti-human secondary mono or polyclonal antibody, etc.) which can bind more than one time to the first detection agent, thus amplifying the signal of detection. The secondary detection agent is then quantified by the use of some other means (e.g., TMB/pcroxidase, scintillation counting, fluorescent probes, etc.). Additionally, the assay detects FOLRI and is not negatively impacted by the presence of Movl9, IMGN853, other FOLRI. family members, or folic acid.

[0274] The assays of the present invention include assays both to select patients eligible to receive FOLRI-based therapy and assays to monitor patient response. Assays for response prediction are run before therapy selection, and levels of FOLRI may impact therapy decisions. For monitoring patient response, the assay is run at the initiation of therapy

&amp;#1470; - to establish baseline (or predetermined) levels of FOLRI in the sample. The same sample is then assayed and the levels of FOLRI compared to the baseline or predetermined levels. As used herein, the term predetermined level refers generally to an assay cutoff value that is used to assess diagnostic results by comparing the assay results against the predetermined level, and where the predetermined level already has been linked or associated with various clinical parameters (e.g., monitoring whether a subject being treated with a drug has achieved an efficacious blood level of the drug, monitoring the response of a subject receiving treatment for cancer with an anti-cancer drug, monitoring the response of a tumor in a subject receiving treatment for said tumor, etc.). The predetermined level may be either an absolute value or a value normalized by subtracting the value obtained from a patient prior to the initiation of therapy. An example of a predetermined level that can be used is a baseline level obtained from one or more subjects that may optionally be suffering from one or more diseases or conditions. The comparison (or informational analysis) of the level of the assayed biomarker with the baseline or predetermined level can be done by an automated system, such as a software program or intelligence system that is part of, or compatible with, the equipment (e.g., computer platform) on which the assay is carried out. Alternatively, this comparison or informational analysis can be done by a physician. In one embodiment, where the levels remain the same or decrease, the therapy may be effective and can be continued. Where significant increase over baseline level (or predetermined level) occurs, the patient may not be responding. In another embodiment, an increase in shed FOLRI levels may be indicative of increased cell death and increased release of the shed FOLRL In this embodiment, an increase in shed FOLRI is indicative of therapeutic efficacy.

[0275] The assays of the present invention can be performed by any protein assay methods. Protein assay methods useful in the invention are well known in the art and include immunoassay methods involving binding of a specific unlabeled or labeled antibody or protein to the expressed protein or fragment of FOLRL Useful immunoassay methods include both solution phase assays conducted using any format known in the art, such as, but not limited to, Biacorc, time resolved fluorescence energy transfer (TR-FRET), an ELISA format, (sandwich, forward and reverse competitive inhibition) or a fluorescence polarization format, and solid phase assays such as immunohistochemistry. The FOLRI antibodies and antigen-binding fragments thereof provided herein are particularly useful for these immunoassay methods because, for example, they arc able to detect a dynamic range of FOLRL

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VII. Circulating Tumor Cell Assays

10276] The anti-FOLRl antibodies described herein can also be used for the detection of FOLRI in a circulating tumor cell assay. Circulating tumor cells (CTCs) are cells that have shed into the vasculature from a tumor and circulate in the bloodstream. CTCs are present in circulation in extremely low numbers. In general, CTCs are enriched from patient blood or plasma by various techniques known in the art. CTCs can be stained for specific markers using methods known in the art including, but not limited to, flow cytometry-based methods and IHC-based methods. CTCs may be stained for protein markers unique to the tumor cells, which allows for the identification and distinction of CTCs from normal blood cells. CTCs can also be stained for FOLRI using the antibodies provided herein including but not limited to 2.1, 5.7, and 9.20. CTC analysis can also include quantitative analysis of the number of CTCs and/or the number of FOLRI positive CTCs. The FOLRI antibodies described herein can be used to stain the CTCs isolated from a subject having a cancer to measure the FOLRI present in the CTCs. An increase in FOLRI expressing CTCs can help identify the subject as having a cancer that is likely to respond to FOLRI based therapy or allow for optimization of a therapeutic regimen with a FOLRI antibody or immunoconjugate. CTC FOLRI quantitation can provide information on the stage of tumor, response to therapy and/or disease progression. It can be used as prognostic, predictive or pharmacodimamic biomarker. In addition, staining of CTCs for FOLRI using the antibodies provided herein, can be used as a liquid biopsy either alone or in combination with additional tumor marker analysis of solid biopsy samples.

VIII. Detection !0277] The present invention further provides antibodies against FOLRI, generally of the monoclonal type, that are linked to at least one agent to form a detection antibody conjugate. In order to increase the efficacy of antibody molecules as diagnostic it is conventional to link or covalently bind or complex at least one desired molecule or moiety. Such a molecule or moiety may be, but is not limited to, at least one reporter molecule. A reporter molecule is defined as any moiety that may be detected using an assay. Non-limiting examples of reporter molecules that have been conjugated to antibodies include enzymes, radiolabels, haptens, fluorescent labels, phosphorescent molecules, chemiluminescent molecules, chromophores, luminescent molecules, photoaffmity molecules, colored particles and/or ligands, such as biotin.

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[0278] Certain examples of antibody conjugates are those conjugates in which the antibody or antigen-binding fragment thereof provided herein is linked to a detectable label. Detectable labels are compounds and/or elements that can be detected due to their specific functional properties, and/or chemical characteristics, the use of which allows the antibody or antigen-binding fragment to which they are attached to be detected, and/or further quantified if desired.

[0279] Many appropriate imaging agents are known in the art, as are methods for their attachment to antibodies (see, e.g., U.S. Pat. Nos. 5,021,236; 4,938,948; and 4,472,509, each incorporated herein by reference). The imaging moieties used can be paramagnetic ions; radioactive isotopes; fluorochromes; NMR-detectable substances; and/or X-ray imaging, for example.

[0280] Exemplary fluorescent labels contemplated for use as binding agent (e.g., antibody) conjugates include Alexa 350, Alexa 430, Alexa 488, AMCA, BODIPY 630/650, BODIPY 650/665, BODIPY-FL, BODIPY-R6G, BODIPY-TMR, BODIPY-TRX, Cascade Blue, Cy3, Cy5,6-FAM, Dylight 488, Fluorescein Isothiocyanate (FITC), Green fluorescent protein (GFP), EIEX, 6-JOE, Oregon Green 488, Oregon Green 500, Oregon Green 514, Pacific Blue, Phycoerythrin, REG, Rhodamine Green, Rhodamine Red, tetramethyl rhodamin (TMR) Renographin, ROX, TAMRA, TET, Tetramethy!rhodamine, Texas Red, and derivatives of these labels (i.e., halogenated analogues, modified with isothiocynate or other linker for conjugating, etc.), for example. An exemplary radiolabel is tritium.

[0281] Antibody or antigen-binding fragment detection conjugates contemplated in the present invention include those for use in vitro, where the antibody or fragment is linked to a secondary binding ligand and/or to an enzyme (an enzyme tag) that will generate a colored product upon contact with a chromogenic substrate. The FOLRI antibodies and antigen-binding fragments thereof provided herein arc particularly useful for conjugates methods because, for example, they arc able io detect a dynamic range of FOLRI. Examples of suitable enzymes include urease, alkaline phosphatase, (horseradish) hydrogen peroxidase and/or glucose oxidase. In some embodiments, secondary binding ligands are biotin and/or avidin and streptavidin compounds. The use of such labels is well known to those of skill in the art and are described, for example, in U.S. Pat. Nos. 3,817,837; 3,850,752; 3,939,350; 3,996,345; 4,277,437; 4,275,149 and 4,366,241; each incorporated herein by reference.

[0282] Molecules containing azido groups may also be used to form covalent bonds to proteins through reactive nitrene intermediates that are generated by low intensity ultraviolet light (Potter &amp; Haley, 1983). In particular, 2- and 8-azido analogues of purine

&amp;#1470; - nucleotides have been used as site-directed photoprobes to identify nucleotide binding proteins in crude cell extracts (Owens &amp; Haley, 1987; Atherton et al., 1985). The 2- and 8azido nucleotides have also been used to map nucleotide binding domains of purified proteins (Khatoon et aL, 1989; King et al., 1989; and Dholakia et al., 1989) and can be used as antibody binding agents.

[0283] Several methods are known in the art for the attachment or conjugation of an antibody to its conjugate moiety. Some attachment methods involve the use of a metal chelate complex employing, for example, an organic chelating agent such a diethylcnetriaminepentaacetic acid anhydride (DTPA); ethylcnctriaminetetraacetic acid; Nchloro-p-toluenesulfonamide; and/or tetrachloro-3a-6a-diphenylg]ycouril-3 attached to the binding agent (e.g., antibody) (U.S. Pat. Nos. 4,472,509 and 4,938,948, each incorporated herein by reference). Monoclonal antibodies may also be reacted with an enzyme in the presence of a coupling agent such as glutaraldehyde or periodate. Protein binding (e.g., antibody) conjugates with fluorescein markers are prepared in the presence of these coupling agents or by reaction with an isothiocyanate. In U.S. Pat. No. 4,938,948, imaging of breast tumors, for example, is achieved using monoclonal antibodies, and the detectable imaging moieties are bound to the antibody using linkers such as methyl-p-hydroxybenzimidate or Nsuccinimidyl -3 -(4-hydroxyphenyl)propionate.

[0284] In other embodiments, derivatization of immunoglobulins by selectively introducing sulfhydryl groups in the Fc region of an immunoglobulin using reaction conditions that do not alter the antibody combining site arc contemplated. Antibody conjugates produced according to this methodology arc disclosed to exhibit improved longevity, specificity and sensitivity (U.S. Pat. No. 5,196,066, incorporated herein by reference). Sitc-specific attachment of effector or reporter molecules, wherein the reporter or effector molecule is conjugated to a carbohydrate residue in the Fc region, have also been disclosed in the literature (O’Shanncssy et al., 1987).

[0285] In other embodiments of the invention, immunoglobulins arc radiolabeled with nuclides such as tritium. In additional embodiments, nanogold particles (such as sizes from about 0.5 nm-40 nm) and/or Quantum Dots (Hayward, Calif.) are employed.

[0286] When a sandwich assay format is used, the capture antibody will be unlabelcd.

The detection antibody will be either directly labeled, or detected indirectly by addition (after washing off excess detection antibody) of a molar excess of a second, labeled antibody directed against the first antibody.

&amp;#1470;

[0287] The label used for the detection antibody is any detectable functionality that does not interfere with the binding of the FOLRI antibodies. Examples of suitable labels are those numerous labels known for use in immunoassay, including moieties that may be detected directly, such as fluorochrome, chemiluminescent, and radioactive labels, as well as moieties, such as enzymes, that must be reacted or derivatized to be detected. Examples of such labels include the radioisotopes 32P, 14C, 125I, 3H, and 131I, fluorophores such as rare earth chelates or fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, luciferases, e.g., firefly luciferase and bacterial luciferase (U.S. Pat. No. 4,737,456), luciferin, 2,3-dihydrophthalazinediones, horseradish peroxidase (HRP), alkaline phosphatase, β-galactosidasc, glucoamylasc, lysozyme, saccharide oxidases, e.g., glucose oxidase, galactose oxidase, and glucose phosphate dehydrogenase, heterocyclic oxidases such as uricase and xanthine oxidase, coupled with an enzyme that employs hydrogen peroxide to oxidize a dye precursor such as HRP, lactoperoxidasc, or microperoxidase, biotin/avidin, biotin/streptavidin, biotin/Streptavidin-β-galactosidase with MUG, spin labels, bacteriophage labels, stable free radicals, and the like. As noted herein, the fluorimetric detection is one example.

[0288] Conventional methods are available to bind these labels covalently to proteins or polypeptides. For instance, coupling agents such as dialdehydes, carbodiimides, dimaleimides, bis-imidates, bis-diazotized benzidine, and the like may be used to tag the antibodies with the herein-described fluorescent, chemiluminescent, and enzyme labels. See, for example, U.S. Pat. Nos. 3,940,475 (fluorimetry) and 3,645,090 (enzymes); Hunter et al. Nature 144:945 (1962); David et al. Biochemistry 13:1014-1021 (1974); Pain ct al. J, Immunol. Methods 40:219-230 (1981); andNygren J, Histochem. and Cytocliem. 30:407-412 (1982). In certain embodiments, labels herein are fluorescent to increase amplification and sensitivity to 8 pg/ml, more preferably biotin with streptavidin&amp;#1470;P&amp;#1470;galactosidase and MUG for amplifying the signal. In certain embodiments, a colorimetric label is used, e.g., where the detectable antibody is biotinylated and the detection means is avidin or streptavidinperoxidase and 3,3',5,5’-tetramcthyl benzidine.

[0289] The conjugation of such label, including the enzymes, to the antibody is a standard manipulative procedure for one of ordinary skill in immunoassay techniques. See, for example, O'Sullivan et al. Methods for the Preparation of Enzyme-antibody Conjugates for Use in Enzyme Immunoassay, in Methods in Enzymology, ed. J. J. Langonc and H. Van Vunakis, Vol. 73 (Academic Press, New York, N.Y., 1981), pp. 147-166.

[0290] Following the addition of last labeled antibody, the amount of bound antibody is determined by removing excess unbound labeled antibody through washing and then measuring the amount of the attached label using a detection method appropriate to the label, and correlating the measured amount with the amount of shed FOLRI in the biological sample. For example, in the case of enzymes, the amount of color developed and measured will be a direct measurement of the amount of shed FOLRI present. Specifically, if HRP is the label, the color can be detected using the substrate 3,3',5,5'-tetramethyl benzidine at 450 nm absorbance.

IX. Substrates and Indicators

[0291] The use of substrates and indicators is contemplated for detection of FOLRI.

[0292] Horseradish peroxidase (HRP) is an enzyme that first forms a complex with hydrogen peroxide and then causes it to decompose, resulting in water and atomic oxygen. Like many other enzymes, HRP and some HRP-like activities can be inhibited by excess substrate. The complex formed between HRP and excess hydrogen peroxide is catalytically inactive and in the absence of an electron donor (e.g. chromogenic substance) is reversibly inhibited. It is the excess hydrogen peroxide and the absence of an electron donor that brings about quenching of endogenous HRP activities. When used in assay systems, HRP can also be used to convert a defined substrate into its activated chromagen, thus causing a color change. The HRP enzyme can be conjugated to an antibody, peptide, polymer, or other molecule by a number of methods that are known in the art. Adding glutaraldehyde to a solution containing an admixture of HRP and antibody will result in more antibody molecules being conjugated to each other than to the enzyme. In the two-step procedure, HRP reacts with the bifunctional reagents first. In the second stage, only activated HRP is admixed with the antibody, resulting in much more efficient labeling and no polymerization. HRP is also conjugated to (strept)avidin using the two-step glutaraldehyde procedure. This form is used in procedures where LAB and LSAB are substrates, for example. Conjugation with biotin also involves two steps, as biotin must first be derivatized to the biotinyl-N-hydroxysuccinimidc ester or to biotin hydrazide before it can be reacted with the epsilonamino groups of the HRP enzyme.

[0293] 3,3’-diaminobenzidine (DAB) is a substrate for enzymes such as HRP that produces a brown end product that is highly insoluble in alcohol and other organic solvents. Oxidation of DAB also causes polymerization, resulting in the ability to react with osmium tetroxidc, and thus increasing its staining intensity and electron density. Of the several metals

&amp;#1470; - and methods used to intensify the optical density of polymerized DAB, gold chloride in combination with silver sulfide appears to be the most successful.

[0294] 3-Amino ethylcarbazole (AEC), is a substrate for enzymes such as HRP, and upon oxidation, forms a rose-red end product that is alcohol soluble. Therefore, specimens processed with AEC must not be immersed in alcohol or alcoholic solutions (e.g., Hams' hematoxylin). Instead, an aqueous counterstain and mounting medium should be used. [0295] 4-Chloro-l-naphthol (CN) is a substrate for enzymes such as HRP that precipitates as a blue end product. Because CN is soluble in alcohol and other organic solvents, the specimen must not be dehydrated, exposed to alcoholic counterstains, or coverslipped with mounting media containing organic solvents. Unlike DAB, CN tends to diffuse from the site of precipitation.

[0296] p-Phenylenediamine dihydrochloride/pyrocatcchol (Hanker-Yates reagent) is a substrate for enzymes such as HRP that gives a blue-black reaction product that is insoluble in alcohol and other organic solvents. Like polymerized DAB, this reaction product can be osmicated. Varying results have been achieved with Hanker-Yates reagent in immunoperoxidase techniques.

[0297] Calf intestine alkaline phosphatase (AP) (molecular weight 100 kD) removes (by hydrolysis) and transfers phosphate groups from organic esters by breaking the P-0 bond; an intermediate enzyme-substrate bond is briefly formed. The chief metal activators for AP are Mg++, Mn++ and Ca++.

[0298[ AP had not been used extensively in immunohistochemistry until publication of the unlabeled alkaline phosphatase-anti-alkaline phosphatase (APAAP) procedure. The soluble immune complexes utilized in this procedure have molecular weights of approximately 560 kD. The major advantage of the APAAP procedure compared to the peroxidase anti-peroxidase (PAP) technique is the lack of interference posed by endogenous peroxidase activity. Endogenous peroxidase can be blocked using a dilute solution of hydrogen peroxide. Because of the potential distraction of endogenous peroxidase activity on PAP staining, the APAAP technique is recommended for use on blood and bone marrow smears. Endogenous alkaline phosphatase activity from bone, kidney, liver and some white cells can be inhibited by the addition of 1 mM levamisole to the substrate solution, although 5 mM has been found to be more effective. Intestinal alkaline phosphatases are not adequately inhibited by levamisole.

[0299] In the immunoalkaline phosphatase staining method, the enzyme hydrolyzes naphthol phosphate esters (substrate) to phenolic compounds and phosphates. The phenols

&amp;#1470; - couple to colorless diazonium salts (chromogen) to produce insoluble, colored azo dyes. Several different combinations of substrates and chromogens have been used successfully.

[0300] Naphthol AS-MX phosphate AP substrate can be used in its acid form or as the sodium salt. The chromogen substrate Fast Red TR and Fast Blue BB produce a bright red or blue end product, respectively. Both are soluble in alcoholic and other organic solvents, so aqueous mounting media must be used. Fast Red TR is preferred when staining cell smears.

[0301] Additional exemplary substrates include naphthol AS-B1 phosphate, naphthol AS-TR phosphate and 5-bromo chloro3&amp;#1524;-indoxyl phosphate (BCIP). Other possible chromogens include Fast Red LB, Fast Garnet GBC, Nitro Blue Tetrazolium (NBT) and iodonitrotetrazolium Violet (INT), for example.

X. Immunodetection Methods

[0302] In still further embodiments, the present invention concerns immunodetection methods for binding, purifying, removing, quantifying and/or otherwise generally detecting biological components such as a ligand as contemplated by the present invention. The antibodies prepared in accordance with the present invention may be employed. Some immunodetection methods include immunohistochemistry, flow cytometry, enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA), immunoradiometric assay, fluoroimmunoassay, chemiluminescent assay, bioluminescent assay, and Western blot to mention a few. The steps of various usefill immunodetection methods have been described in the scientific literature, such as, e.g., Doolittle Μ. H and Ben-Zeev O, Methods Mol Biol. 1999;109:215-37; Gulbis B and Galand P, Hum Pathol. 1993 Dec;24(12):1271-85; and De Jager R et al., Semin Nucl Med. 1993 Apr;23(2): 165-79, each incorporated herein by reference.

[0303] In general, the immunobinding methods include obtaining a sample suspected of comprising ligand protein, polypeptide and/or peptide, and contacting the sample with a first ligand binding agent (e.g., an anti-ligand antibody) in accordance with the present invention, as the case may be, under conditions effective to allow the formation of immunocomp lexes.

[0304] In terms of antigen detection, the biological sample analyzed may be any sample in which it is desirable to detect FOLRI such as fluidic extract, blood, plasma, serum, spinal fluid, lymph fluid, tissue section or specimen, homogenized tissue extract, biopsy aspirates, a cell, separated and/or purified forms FOLRl-containing compositions, or any

&amp;#1470; - biological fluid. In some embodiments, blood, plasma, or lymph samples or extracts are used.

[0305] Contacting the chosen biological sample with the antibody under effective conditions and for a period of time sufficient to allow the formation of immune complexes (primary immune complexes) is generally a matter of simply adding the antibody composition to the sample and incubating the mixture for a period of time long enough for the antibodies to form immune complexes with, i.e., to bind to, any ligand protein antigens present. After this time, the sample-antibody composition, such as a tissue section, ELISA plate, dot blot or western blot, will generally be washed to remove any non-specifically bound antibody species, allowing only those antibodies specifically bound within the primary immune complexes to be detected.

[0306] In general, the detection of immunocomp lex formation is well known in the art and may be achieved through the application of numerous approaches. These methods are generally based upon the detection of a label or marker, such as any of those radioactive, fluorescent, biological and enzymatic tags. U.S. Patents concerning the use of such labels include U.S. Pat. Nos. 3,817,837; 3,850,752; 3,939,350; 3,996,345; 4,277,437; 4,275,149 and 4,366,241, each incorporated herein by reference. Of course, one may find additional advantages through the use of a secondary binding ligand such as a second antibody and/or a biotin/avidin ligand binding arrangement, as is known in the art.

[0307] The anti-ligand antibody employed in the detection may itself be linked to a detectable label, wherein one would then simply detect this label, thereby allowing the amount of the primary immune complexes in the composition to be determined. Alternatively, the first antibody that becomes bound within the primary immune complexes may be detected by means of a second binding agent that has binding affinity for the antibody. In these cases, the second binding agent may be linked to a detectable label. The second binding agent is itself often an antibody, which may thus be termed a secondary antibody. The primary immune complexes are contacted with the labeled, secondary binding agent, or antibody, under effective conditions and for a period of time sufficient to allow the formation of secondary immune complexes. The secondary immune complexes are then generally washed to remove any non-specifically bound labeled secondary antibodies or ligands, and the remaining label in the secondary immune complexes is then detected.

[0308] Further methods include the detection of primary immune complexes by a two-step approach. A second binding agent, such as an antibody, that has binding affinity for the antibody is used to form secondary immune complexes, as described herein. After

WO 2015/031815 “ 85 - washing, the secondary immune complexes are contacted with a third binding agent or antibody that has binding affinity for the second antibody, again under effective conditions and for a period of time sufficient to allow the formation of immune complexes (tertiary immune complexes). The third ligand or antibody is linked to a detectable label, allowing detection of the tertiary immune complexes thus formed. This system may provide for signal amplification if this is desired.

[0309] In another embodiment, a biotinylated monoclonal or polyclonal antibody is used to detect the target antigen(s), and a second step antibody is then used to detect the biotin attached to the complexed biotin. In that method the sample to be tested is first incubated in a solution comprising the first step antibody. If the target antigen is present, some of the antibody binds to the antigen to form a biotinylated antibody/antigen complex. The antibody/antigen complex is then amplified by incubation in successive solutions of streptavidin (or avidin), biotinylated DNA, and/or complementary biotinylated DNA, with each step adding additional biotin sites to the antibody/antigen complex. The amplification steps are repeated until a suitable level of amplification is achieved, at which point the sample is incubated in a solution comprising the second step antibody against biotin. This second step antibody is labeled, as for example with an enzyme that can be used to detect the presence of the antibody/antigen complex by histoenzymology using a chromogen substrate. With suitable amplification, a conjugate can be produced that is macroscopically visible.

[0310] In one embodiment, immunohistochemistry (IHC) is used for immunological detection. Using IHC, detection of FOLRI in a sample can be achieved by targeting a sample with a probe e.g., an. anti-FOLRl antibody. The probe can be linked, either directly or indirectly to a detectable label or can be detected by another probe that is linked, either directly or indirectly to a detectable label.

[0311] In some embodiments, IHC can distinguish between different levels of protein expression, e.g., calibrated IHC. In some embodiments, the IHC can distinguish staining intensity for samples having low FOLRI, intermediate FOLRI, or high FOLRI expression.

[0312] In one embodiment, immunological detection (by immunohistochemistry) of

FOLRI is scored for both intensity and uniformity (percent of stained cells - membrane only). Comparative scales for FOLRI expression for intensity correlate as 0 - Negative, 0-1

- Very Weak, 1 - Weak, 1-2 - Weak to Moderate, 2 Moderate, 2-3 - Moderate to Strong, 3

- Strong, 3+ - Very Strong. Quantitatively, Score 0 represents that no membrane staining is observed. Score 1 represents that a faint/barely perceptible membrane staining is detected.

For Score 2, a weak to moderate complete membrane staining is observed. Lastly, Score 3

—&amp;#1512; .:: .:. ::.7----- :. &amp;#1524;. ;.:. &amp;#1470;’ &amp;#1488;;&amp;#1523;.?.::'.':/ 1:

&#1470; - (or 3+) represents that moderate to complete membrane staining is observed. Those samples with 0 or 1 score for FOLRI expression can be characterized as not having elevated FOLRI expression, whereas those samples with 2 or 3 scores can be characterized as overexpressing or having elevated FOLRI. In another embodiment, using the antibodies, antigen-binding fragments thereof, or polypeptides provided herein, those samples with a 0 score for FOLRI expression can be characterized as not having elevated FOLRI expression, those samples with a 1 score can be characterized as having increased expression of FOLRI, and those samples with 2 or 3 scores can be characterized as overexpressing or having elevated FOLRI. [0313] Samples overexpressing FOLRI can also be rated by immunohistochemical scores corresponding to .the number of copies of FOLRI molecules expressed per cell, or antibodies bound per cell (ABC), and can been determined biochemically. Comparative scales for FOLRI uniformity (percent cell membrane staining) are as follows: Negative = 0%; Focal = <25%; heterogeneous (hetero) = 25-75%, and homogeneous (homo) = >75%.

[0314] In one embodiment, immunological detection (by immunohistochemistry) of FOLRI is scored using H-scores. H-scores combine staining intensity scores (e.g., a score of 0 to 3, wherein 0 represents no staining, and 3 represents strong staining) with the percentage of cells that are positive for membrane staining (i.e., uniformity). An H-score can be cacluated as follows:

H score = [0* (percentage of cells staining at intensity 0)] + [1 * (percentage of cells staining at intensity 1)] + [2 *(percentage of cells staining at intensity 2)] + [3 *(percentage of cells staining at intensity 3)]. Accordingly, an H-score can range from 0 (no cell membranes staining) to 300 (all cell membranse staining at intensity 3).

[0315] In one embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when the H-score for FOLR expression in a tumor sample from the subject is at least 50. In one embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when the H-score for FOLR expression in a tumor sample from the subject is at least 75. In one embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when the H-scorc for FOLR expression in a tumor sample from the subject is at least 100. In one embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when the H-score for FOLR expression in a tumor sample from the subject is at least 125. In one embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., 1MGN853) when the 11

&amp;#1470; - score for FOLR expression in a tumor sample from the subject is at least 150. In one embodiment, a subject having cancer is identified as a candidate for treatment with an antiFOLRI treatment regimen (e.g., 1MGN853) when the H-score for FOLR expression in a tumor sample from the subject is at least 175. In one embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when the H-score for FOLR expression in a tumor sample from the subject is at least 200. In another embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in a tumor sample from the subject is at least 225. In another embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-scorc for FOLR expression in a tumor sample from the subject is at least 250. In another embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in a tumor sample from the subject is at least 275. In another embodiment, a subject having cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in a tumor sample from the subject is 300.

[0316] In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is 75 to 300. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is at least 75. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is at least 100. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is at least 125. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the I !score for FOLR expression in an ovarian tumor sample from the subject is at least 150. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., 1MGN853) when the H-scorc for FOLR expression in an ovarian tumor sample from the subject is at least 175. In another embodiment, a subject

&amp;#1470; - having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is at least 200. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is at least 225. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is at least 250. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is at least 275. In another embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an ovarian tumor sample from the subject is 300.

[0317] In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-scorc for FOLR expression in an NSCLC tumor sample from the subject is 50 to 300. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an antiFOLRI regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 50. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 75. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-scorc for FOLR expression in an NSCLC tumor sample from the subject is at least 100. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an antiFOLRI regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 125. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., 1MGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 150. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 175. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an antiWO 2015/031815 89 &amp;#1470; “

FOLRI regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 200. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 225, In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when, the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 250. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an antiFOLRI regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is at least 275. In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an NSCLC tumor sample from the subject is 300.

[0318] In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is 50 to 300. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 50. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 75. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-scorc for FOLR expression in an endometrial tumor sample from the subject is at least 100. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 125. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 150. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., 1MGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 175. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl

WO 2015/031815 &amp;#1470; 90 &amp;#1470; regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial. tumor sample from the subject is at least 200. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 225. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 250. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is at least 275. In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl regimen (e.g., IMGN853) when the H-score for FOLR expression in an endometrial tumor sample from the subject is 300.

[0319] By way of example, an H-score in a subject having ovarian cancer may be as follows:

H score = (75% at intensity 0) + (0% at intensity 1) + (0% at intensity 2) + (25% at intensity 3)-75; or

H score = (0% at intensity 0) + (75% at intensity 1) + (0% at intensity 2) + (25% at intensity 3)-150.

In another example, an H-score in a subject having endometrial cancer may be as follows:

H score - (75% at intensity 0) + (0% at intensity 1) + (25% at. intensity 2) + (0% at intensity 3) - 50; or

H score - (0% at intensity 0) + (75% at intensity 1) + (25% at intensity 2) + (0% at intensity 3)-125.

In all four examples above, the subject could be identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., 1MGN853).

[0320] In one embodiment, immunological detection (by immunohistochemistry) of FOLRI is scored using percent positivity and intensity across a sample. In this embodiment, selection for treatment with an anti-FOLRl treatment regimen is based on the percentage of cells in a sample that are found to express membrane FOLRI at a specified level that reflects

WO 2015/031815 &#1470;91&#1470; both the staining intensity (e.g., 1, 2, or 3) and uniformity (e.g., heterogeneous or homogeneous (see Table 11)). For example, a sample having at least 25% (i.e., 25-75% or > 75%) of the cells staining for FOLRI positivity at 3 could be characterized as 3 hetero and 3 homo or, collectively, as at least 25% positive at 3.

[0321] Tn one embodiment, a subject having ovarian cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when at least 25% of the FOLRI membrane expression in a tumor sample from the subject has an intensity score of 3 by IHC. In one embodiment, the IHC is performed using the FOLRl-2,1 antibody.

[0322] In another embodiment, a subject having endometrial cancer is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when at least 25% of the FOLR membrane expression in a tumor sample from the subject has an intensity score of at least 2 by IHC. In one embodiment, the IHC is performed using the FOLRI-2.1 antibody.

[0323] In another embodiment, a subject having NSCLC is identified as a candidate for treatment with an anti-FOLRl treatment regimen (e.g., IMGN853) when at least 25% of the FOLR membrane expression in a tumor sample from the subject has an intensity score of at least 2 by IHC. In one example, the IHC is peformed using the FOLRI-2.1 antibody for IHC.

[0324] IHC can be performed manually or using an automated system (e.g., using an automated stainer). IHC can be performed on cells, cell pellets, tissues, preparations from blood, plasma, serum, or lymph fluid, etc. In some embodiments, the samples are fixed samples. In some embodiments, the samples are paraffin embedded samples. Tn some embodiments, the samples are formalin fixed and paraffin embedded samples.

[0325] In one embodiment, flow cytometry is used for immunological detection. Thus, for example, the number of antibodies bound per cell (ABC) can be assessed using flow cytometry. A high number of anti-FOLRl antibodies bound per cell can indicate high FOLRI expression levels and a high likelihood to be susceptible to treatment with an antiFOLRI antibody or immunoconjugate thereof.

XL Compositions and Kits

[0326] Also provided by the invention arc compositions and kits for use in the practice of the present invention as disclosed herein. Such kits may comprise containers, each with one or more of the various reagents (typically in concentrated form) utilized in the

methods, including, for example, one or more binding agents (antibodies), already attached to a marker or optionally with reagents for coupling a binding agent to an antibody (as well as the marker itself), buffers, and/or reagents and instrumentation for the isolation (optionally by microdissection) to support the practice of the invention. A label or indicator describing, or a set of instructions for use of, kit components in a ligand detection method of the present invention, will also be typically included, where the instructions may be associated with a package insert and/or the packaging of the kit or the components thereof.

[0327] In still further embodiments, the present invention concerns immunodetection kits for use with the immunodetection methods described herein. As the antibodies are generally used to detect FOLRI, he antibodies will generally be included in the kit. The immunodetection kits will thus comprise, in suitable container means, a first antibody that binds to FOLRI and/or optionally, an immunodetection reagent and/or further optionally, a FOLRI protein or cell sample containing FOLRI.

[G328] The immunodetection reagents of the kit may take any one of a variety of forms, including those detectable labels that are associated with and/or linked to the given antibody. Detectable labels that are associated with and/or attached to a secondary binding ligand are also contemplated. Exemplary secondary ligands are those secondary antibodies that have binding affinity for the first antibody.

[0329] Further suitable immunodetection reagents for use in the present kits include the two-component reagent that comprises a secondary antibody that has binding affinity for the first antibody, along with a third antibody that has binding affinity for the second antibody, the third antibody being linked to a detectable label. As noted herein, a number of exemplary labels are known in the art and/or all such labels may be suitably employed in connection with the present invention.

[0330] The kits may further comprise a therapeutic agent for the treatment of cancer, such as an anti-FOLRl immunoconjugatc.

[0331] The kit may further comprise an a FOLRI detection reagent used to measure FOLRI expression in a subject comprising a FOLRI detection reagent, and instructions for use. In one embodiment, the FOLRI detection reagent comprises a FOLRI binding peptide or anti- FOLRI antibody. In another embodiment, the kit further comprises a secondary antibody which binds the anti-FOLRl antibody.

[0332] In one embodiment the FOLRl-spccific antibody is included at a concentration of about 0.1 to about 20 pg/mL, about 0.1 to about 15 pg/mL, about 0.1 to about 10 pg/mL, about 0.5 to about 20 pg/mL, about 0.5 to about 15 pg/mL, about 0.5 to

&amp;#1470; - about 10 pg/mL, about 1 to about 20 pg/mL, about 1 to about 15 pg/mL, about .1 to about 10 μg/mL, about 2 to about 20 pg/mL, about 2 to about 15 pg/mL, or about 2 to about 10 pg/mL. In another embodiment, the FOLRI-specific antibody is included at a concentration of about 1.5 pg/mL, about 2 pg/mL, about 3 pg/mL, about 4 pg/mL, about 5 pg/mL, about 6 pg/mL, about 7 pg/mL, about 8 pg/mL, about 9 pg/mL, or about 10 pg/mL. In another embodiment, the FOLRI-specific antibody is included at a concentration of about 2 pg/mL. In another embodiment, the FOLRI-specific antibody is included at a concentration of about 10 pg/mL.

[0333] In another embodiment, the antibody is included in concentrated solution with instructions for dilutions to achieve a final concentration of about 1 to about 20 pg/mL, about 1 to about 15 pg/mL, about 1 to about 10 pg/mL, about 2 to about 20 pg/mL, about 2 to about 15 pg/mL, or about 2 to about 10 pg/mL. In another embodiment, the antibody is included in concentrated solution with instructions for dilutions to achieve a final concentration of about 1.5 pg/mL, about 2 pg/mL, about 3 pg/mL, about 4 pg/mL, about 5 pg/mL, about 6 pg/mL, about 7 pg/mL, about 8 pg/mL, about 9 pg/mL, or about 10 pg/mL. In another embodiment, the antibody is included in concentrated solution with instructions for dilutions to achieve a final concentration of about 2 pg/mL. In another embodiment, the antibody is included in concentrated solution with instructions for dilutions to achieve a final concentration of about 10 pg/ml.

[0334] In another embodiment, the kit further comprises a detection reagent selected from the group consisting of: an enzyme, a fluorophore, a radioactive label, and a luminophorc. In another embodiment, the detection reagent is selected from the group consisting of: biotin, digoxigenin, fluorescein, tritium, and rhodamine.

[0335] The kit can also include instructions for detection and scoring of FOLRI expression. The kit can. also include control or reference samples. Non-limiting examples of control or reference samples include cell pellets or tissue culture cell lines derived from normal (normal control) or tumor (positive control) samples. Exemplary cell lines include cell lines stably or transiently transfected with an expression vector that expresses FOLRI. Additional examples include cell pellets and tissue samples described in the Examples.

[0336] In some embodiments, a kit is a packaged combination including the basic elements of: (a) capture reagents comprised of the monoclonal antibodies against human FOLRI; and (b) detection reagents which can also comprise FOLRI monoclonal antibodies, but can also comprise detectable (labeled or unlabeled) antibodies that bind to FOLRI. These basic elements are defined herein.

&amp;#1470; -

[0337] In one embodiment, the kit further comprises a solid support for the capture reagents, which can be provided as a separate element or on which the capture reagents are already immobilized. Hence, the capture antibodies in the kit can be immobilized on a solid support, or they can be immobilized on such support that is included with the kit or provided separately from the kit.

[0338] In one embodiment, the capture reagent is coated on a microtiter plate. The detection reagent can be labeled antibodies detected directly or unlabeled antibodies that are detected by labeled antibodies directed against the unlabeled antibodies raised in a different species. Where the label is an enzyme, the kit will ordinarily include substrates and cofactors required by the enzyme, and where the label is a fluorophore, a dye precursor that provides the detectable chromophore. Where the detection reagent is unlabcled, the kit can further comprise a detection means for the detectable antibodies, such as the labeled antibodies directed to the unlabeled antibodies, e.g., in a fluorimetric-detected format. Where the label is an enzyme, the kit will ordinarily include substrates and cofactors required by the enzyme, where the label is a fluorophore, a dye precursor that provides the detectable chromophore, and where the label is biotin, an avidin such as avidin, streptavidin, or streptavidin conjugated to HRP or β-galactosidase with MUG.

[0339] In one embodiment, the capture reagent is the FOLRI antibody 2.1, 5.7, or 9.20 or an antibody comprising the sequences of antibody 2.1, 5.7 or 9.20. In one embodiment, the detection reagent is the FOLRI antibody 2.1, 5.7, or 9.20 or an antibody comprising the sequences of antibody 2.1, 5.7 or 9.20. In another embodiment, the detection reagent FOLRI antibody 2.1, 5.7, or 9.20 or an antibody comprising the sequences of antibody 2.1, 5.7 or 9.20 is biotinylated.

[0340] The kit also typically contains instructions for carrying out the assay, and/or FOLRI protein, or fragments thereof (e.g., FOLRI extracellular domain or the FOLRI extracellular domain and all or a part of the GPI linkage domain) as an antigen standard, as well as other additives such as stabilizers, washing and incubation buffers, and the like. In one embodiment, the FOLR1 antigen standard is a FOLRl&amp;#1470;Fc immunoadhesin. The kit can also include instructions for detection and scoring of FOLRI expression.

[0341] The components of the kit can be provided in predetermined ratios, with the relative amounts of the various reagents suitably varied to provide for concentrations in solution of the reagents that substantially maximize the sensitivity of the assay. Particularly, the reagents can be provided as dry powders, usually lyophilized, including excipients, which

&amp;#1470; - on dissolution will provide for a reagent solution having the appropriate concentration for combining with the sample to be tested.

[0342] Compositions comprising the antibodies or antigen-binding fragments described herein are also provided. In one embodiment, a composition comprises an antiFOLRI antibody or antigen-binding fragment described herein and a buffer, e.g., a buffer that can be used in a detection assay such as FACS, IHC, or ELISA. Such buffers are known to those of ordinary skill in the art and include diluents. By way of example, certain FACS buffers are provided herein, e.g., in the working examples. FACS buffers can also contain, for example, serum or albumin (such as calf serum, goat serum, or BSA) and/or sodium azide. FACS buffers can also contain PBS, EDTA, and/or DNAse or any combination thereof. IHC buffers are also provided herein and known to those of ordinary skill in the art. IHC buffers can contain, for example, casein serum or albumin (such as calf serum, goat serum, or BSA), Tween, or Triton, PBS and/or sodium azide or any combination thereof. ELISA buffers are also provided herein and known to those of ordinary skill in the art. ELISA buffers can contain, for example, serum or albumin (such as calf serum, goat serum, or BSA), non-fat dry milk, casein, and/or gelatin or any combination thereof.

[0343] Embodiments of the present disclosure can be further defined by reference to the following non-limiting examples, which describe in detail preparation of certain antibodies of the present disclosure and methods for using antibodies of the present disclosure. It will be apparent to those skilled in the art that many modifications, both to materials and methods, can be practiced without departing from the scope of the present disclosure.

EXAMPLES

[0344] It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application.

Example 1: Generation of FOLRI hybridomas

[0345] Hybridomas producing anti-human FOLRI monoclonal antibodies that are suitable for immunohistochemistry (IHC) staining (antibodies of the invention) were selected from more than 16,000 hybridomas. The hybridomas were produced by immunization of

&amp;#1470; - wild-type Balb/c mice with different antigens including: formalin fixed 300-19 cells that have been transfected with human FOLRI, human FOLRI-murine IgG2a Fc recombinant protein, and human FOLRI recombinant protein. The immunization with fixed 300-19 cells was conducted by subcutaneous injection of transfected 300-19 cells in PBS (5E6 cells/mouse/injection) in the absence of any adjuvant. The immunization with FOLRI recombinant proteins was done by subcutaneous injection of the protein emulsified in complete Freund’s adjuvant (CFA) or incomplete Freund’s adjuvant for boost (Sigma) or Magic mouse adjuvant (Creative Diagnostics). Generally, mice were immunized five times with two week intervals before receiving a final boost by intraperitoneal injection of the immunogen three days prior to fusion.

[0346] A total of 16 independent fusions (including fusions 352, 353, and 354) were carried out using spleen cells that originated from the immunized wild-type Balb/c mice and murine myeloma P3X63Ag8.653 cells (P3 cells). Cell fusion was conducted using an ECM200 electrofusion machine (BTX Harvard Apparatus) according to standard protocols. Each fusion yielded more than 1,000 hybridomas. Antibodies produced by these hybridomas were screened and confirmed by a FACS based method using denatured FOLRI-positive and FOLRl-negative cells. Of the greater than 16,000 hybridomas screened, 14 hybridomas that were positive by FACS screening were discovered. All of the positive hybridomas originated from mice immunized with human FOLRl-murine IgG2a Fc recombinant protein.

[0347] Of the 14 hybridomas which were initially positive by FACS screening, only ten showed a sufficient IgG concentration for further analysis.

Example 2. Immunohistochemical evaluation of hybridoma supernatants

[0348] Ten of the initial 14 hybridomas were analyzed by IHC. The analysis was performed using the Leica Bond RX Automated Stainer and the reagents and conditions listed in Table 9.

Table 9. IHC Reagents and Assay Conditions

<td> Step</td><td> Action/Reagent (Vendor)</td><td> Time</td>

<td> Bake</td><td> Temperature: 60°C</td><td> 30 Minutes</td>

<td> Dewax</td><td> Bond Dewax Solution (Leica) 100% Ethanol (Pharmco Aaper)</td><td> Fixed</td>

<td> Antigen Retrieval</td><td> Bond Epitope Retrieval 2 (ethylenediaminetetraacctic acid based pH 9.0 solution)</td><td> 20 Minutes</td>

<td> Endogenous Peroxidase Block</td><td> Peroxide (Leica)</td><td> 5 Minutes</td>

WO 2015/031815 &amp;#1470; 97&amp;#1470;

<td> Test Article</td><td> ImmunoGen, Inc. generated antibodies at varying concentrations prepared by diluting in Leica Antibody Diluent</td><td> 15 Minutes</td>

<td rowspan="3"> Detection</td><td> Post Primary Regent (Leica)</td><td> 8 Minutes</td>

<td> Polymer (Leica)</td><td> 8 Minutes</td>

<td> Mixed DAB (Leica)</td><td> 10 Minutes</td>

<td> Counterstain</td><td> Hematoxylin (Leica)</td><td> 5 Minutes</td>

[0349] Slides containing formalin fixed paraffin embeded (FFPE) cells, normal tissues, patient lung tumor biopsies, and patient ovarian tumor biopsies were baked at 60°C and dewaxed using Bond Dewax Solution and 100% Ethanol. Heat induced epitope retrieval using Bond Epitope Retrieval 2 (ethylenediaminetetraacetic acid based pH 9.0 solution) was performed for 20 minutes and endogenous peroxidase was blocked with peroxide for 5 minutes. Slides were incubated with ImmunoGen, Inc. generated antibodies or Leica/Novocastra muIgGl control antibodies at varying concentrations for 15 minutes. Bound antibodies were detected by incubation with the Leica Bond Refine detection system. Following the application of the antibodies, slides were incubated with Post Primary Reagent (rabbit anti-mouse IgG) for 8 minutes, Polymer (goat anti-rabbit polymer) for 8 minutes, and DAB (3,3-diaminobenzidine tetrahydrochloride) for 10 minutes which resulted in a brown color signal. Slides were counterstained with hematoxylin for 5 minutes.

[0350] FFPE tissue samples were derived from human tissue blocks obtained from Proteogenex and the Cooperative Human Tissue Network (CHTN) as outlined below. FFPE cell samples were derived from the KB cell line supplied by American Tissue Culture Collection. Slides containing sections of samples were prepared from FFPE blocks using a microtome set at 5pm and were mounted on positively charged slides. These slides were allowed to air dry overnight prior to staining.

Table 10. FFPE Test Samples

<td> Human Tissue Type</td><td> Commercial Source</td>

<td> Normal Lung</td><td> CHTN</td>

<td> Normal Pancreas</td><td> CHTN</td>

<td> Normal Salivary Gland</td><td> CHTN</td>

<td> Ovarian Papillary Serous Adenocarcinoma</td><td> Proteogenex</td>

<td> Lung Adenocarcinoma</td><td> CHTN</td>

&amp;#1470; -

[0351] FOLRI staining intensity and distribution patterns were scored relative to control IgG staining (non-specific). Intensity was scored on a scale of 0 to 3 where 0 = no staining, 1 - weak staining, 2 - moderate staining, and 3 = strong staining. Uniformity of the staining was scored as negative (no cells exhibit positive staining), focal (<25% of cells stained), heterogeneous (25-75% of cells stained), and homogeneous (>75% of cells stained). The staining intensity and scoring scales are described below. All staining was evaluated by a Board certified pathologist.

Table 11. Intensity and Uniformity of Staining

<td colspan="2"> Intensity (Amount of Membrane Staining)</td>

<td> 0</td><td> Negative</td>

<td> 1</td><td> Weak</td>

<td> 2</td><td> Moderate</td>

<td> 3</td><td> Strong</td>

<td colspan="2"> Uniformity (Percent of Positive Cells)</td>

<td> 0</td><td> Negative</td>

<td> Focal</td><td> &lt;25%</td>

<td> Heterogeneous (hetero)</td><td> 25-75%</td>

<td> Homogeneous (homo)</td><td> &gt;75%</td>

IHC Selection Process to Identify Hybridoma(s) for FFPE FOLRI IHC

[0352] Primary clones positive by FACS on FOLRl-positive denatured cells (ten clones total) were evaluated by IHC. Two clones were obtained from fusion 352 (clones 352.1 and 352.2). Six clones were obtained from fusion 353 (clones 353.1, 353.2, 353.3, 353.5, 353.9, 353.15), and two clones were obtained from fusion 354 (clones 354,1 and 354,2). Hybridoma supernatants were collected from the cultured hybridoma cells and used for the analysis. Antibody concentrations in hybridoma supernatants were determined by ELISA using anti-murine L-chain specific polyclonal antibody to capture murine monoclonal antibody from supernatant and anti-murine Fc-specific polyclonal antibody to detect the captured antibody; murine monoclonal IgGl sample with know concentration was used as a standard to calculate IgG concentration. Cell culture media (undiluted) was shown not to interfere with IHC staining methods (no background/non-specific staining was noted when media was used in place of the primary antibody). Ten supernatants (IMGN 352.1, 352.2, 353.1,353.2, 353.3, 353.5, 353.9, 353.15, 354.1, and 354.2) diluted at varying concentrations up to 10 pg/mL in Leica Antibody Diluent were stained using FOLRI known positive control samples (human normal lung, patient derived ovarian serous papillary adenocarcinoma, and KB cells) and evaluated to identify positive candidate clones (clones depicting acceptable membrane staining and specificity in FOLRI positive samples). Five of the ten clones

&amp;#1470; - exhibited acceptable membrane staining in FOLRI positive samples and good specificity. A suitable staining concentration was experimentally determined for each of the five candidate clones as follows: 353.1 (0.7 pg/mL), 353.2 (2.3 pg/mL), 353.3 (2.3 pg/mL), 353.5 (2 pg/mL and 10 pg/mL), and 353.9 (2 pg/mL and 10 pg/mL). Of the remaining 5 clones, clone 353.15 (stained at 2 and 10 pg/mL) exhibited acceptable membrane staining in KB cells and normal lung tissue; however, cytoplasmic staining only was observed in the patient ovarian tumor tissue tested. Clones 352.1, 352.2, and 354.1 exhibited no visible staining in any samples and clone 354.2 exhibited only apparent non-specific cytoplasmic staining, all considered unacceptable.

[0353] The five candidate clones were further subcloned. Subclones for four clones (353.2, 353.3, 353.5 and 353.9) were successfully identifiedno subclones of clone 353.1 was generated. A total of eight subclones were purified. Two subclones were obtained from clone 353.5 (353.5-7 and 353.5-10). Two subclones were obtained from clone 353.9 (353.9-20 and 353.9-21). Two subclones were obtained from clone 353.3 (353.3-8 and 353.3-9), and two subclones were obtained from clone.353.2 (353.2-1 and 353.2-12). (Note that these subclones are also referred to as 5.7, 5.10, 9.20, 9.21, 3.8, 3.9, 2.1, and 2.12, respectively.) IHC characterization of the subclones was performed using methods described above (Table 9: IHC Reagents and Assay Conditions) at antibody concentrations of 2 and 10 pg/mL. The eight subclones were also sequenced as described in Example 3, below. The candidate subclones were further evaluated to identify and rank for optimal membrane staining and specificity as follows: [353.2-1, 353.2-12], [353.9-20, 353.9-21], [353.5-7,353.5-10], and [353.3-8, 353.3-9] and were selected for further characterization (subclones are bracketed together according to sequence identity as described in Example 3).

[0354] Two subclones were selected for further IHC assay optimization: 353.2-1 and 353.9-20. Both antibodies were used to stain human normal lung, human normal salivary gland, human normal pancreas, patient ovarian cancer biopsies, patient non-small cell lung cancer (NSCLC) biopsies, and patient clear cell renal cell carcinoma biopsies. At optimal conditions (see Table 12 below and Figure 13), both subclones exhibited specific and appropriately sensitive staining in both human normal and patient tumor tissues. Ducts of pancreas, respiratory epithelium of normal lung, and intercalated ducts exhibited positive membrane associated staining. Acinar cells/islets of pancreas, interalveolar connective tissue of lung, and acinar cells of salivary gland expected to be negative did not exhibit positive staining with either subclone. Tumor cells from ovarian cancer, NSCLC, and clear cell renal

cell carcinoma samples expected to be positive exhibited positive membrane associated staining that was localized to the tumor cells. Tumor substructures expected to be negative (stroma, vessels, and lymphocytes) did not exhibit positive staining with 353.2-1 or 353.9-20. Additional staining of normal tissues with 353-2.1 (FOLRI-2.1) are summarized in Table 13, below and shown in Figure 14. Taken together, the IHC characterization data suggests that 353.2-1 and 353.9-20 are specific to FOLRI in FFPE tissues (see Figure 1 and Figure 2).

Table 12. Optimized Assay Conditions

<td> Step</td><td> Action/Reagent (Vendor)</td><td> Time</td>

<td> Bake</td><td> Temperature: 60°C</td><td> 30 Minutes</td>

<td> Dewax</td><td> Bond Dewax Solution (Leica) 100% Ethanol (Pharmco Aaper)</td><td> Fixed</td>

<td> Antigen Retrieval</td><td> Bond Epitope Retrieval 2 (ethylenediaminetetraacetic acid based pH 9.0 solution)</td><td> 20 Minutes</td>

<td> Endogenous Peroxidase Block</td><td> Peroxide (Leica)</td><td> 5 Minutes</td>

<td> Test Article</td><td> IMGN353.2-1 at 1.5gg/mL IMGN353.9-20 at 6.0 gg/mL</td><td> 15 Minutes</td>

<td rowspan="3"> Detection</td><td> Post Primary Regent (Leica)</td><td> 8 Minutes</td>

<td> Polymer (Leica)</td><td> 8 Minutes</td>

<td> Mixed DAB (Leica)</td><td> 10 Minutes</td>

<td> Counterstain</td><td> Hematoxylin (Leica)</td><td> 5 Minutes</td>

Table 13. Optimized Assay Conditions

<td> Normal Tissue, Structure</td><td> 2.1 Staining</td>

<td> Adrenal Gland</td><td> +</td>

<td> Breast lobules</td><td> +</td>

<td> Fallopian tube, surface epithelium</td><td> +</td>

<td> Kidney, tubules</td><td> +</td>

<td> Pancreas, ducts</td><td> +</td>

<td> Pituitary, pituitary cells</td><td> +</td>

<td> Salivary gland, intercalated ducts</td><td> +</td>

<td> Breast, connective tissue</td><td> -</td>

<td> Esophagus submucosa &amp; muscularis</td><td> -</td>

<td> Eye, cornea</td><td> -</td>

<td> Kidney, glomeruli</td><td> -</td>

<td> Lung, interalveolar connective tissue</td><td> -</td>

<td> Liver, hepatocytes .</td><td> -</td>

<td> ..י .. י . Pancreas, acinar cells</td><td> -</td>

<td> Lung, epithelium</td><td> -/+</td>

<td> Stomach, surface epithelium, pits</td><td> ..</td>

Example 3. Characterization of the selected anti-FOLRl antibodies

[0355] As described above in Example 2, of the fourteen hybridoma clones selected based on primary and confirmation FACS screening, ten primary clones were analyzed by immunohistochemistry (IHC) analysis. Of the ten primary clones (i.e., 352.1, 352.2, 353.1, 353.2, 353.3, 353.5, 353.9, 353.15, 354.1, and 354.2), five were positive by IHC (i.e., 353.1, 353.2, and 353.3, 353.5, and 353.9), and all five were derived from the same fusion (fusion 353). Four of the five were successfully subcloned. One subclone of primary clone 353.2 was chosen and named 353.2-1 (2.1). One subclone of primary clone 353.3 was chosen and named 353.3-8 (3.8). One subclone of primary clone 353.5 was chosen and named 353.5-7 (5.7), and two subclones of primary clone 353.9 were chosen and named 353.9-20 (9.20) and 353.9-21 (9.21). Subclones 9.20 and 9.21 were sequenced, and as expected, both subclones had the same sequence. In addition, two of the clones, 2.1 and 9.20 were deposited with ATCC as PTA-120197 and PTA-120196, respectively, on April 16, 2013.

Specificity of the anti-FOLRl antibodies by Western blot

[0356] Specificity of the generated antibodies was analyzed by Western blot with a panel of cell lysates prepared from FOLRI-positive (Igrov-1, Ovcar-3, Caov-3, Wish, and Skov-3) and FOLRI-negative (BxPC3, Panc-1, and ASPC1) cell lines. For the assay, lysates were run in SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane by the standard procedures. The membrane was incubated with the anti-FOLRl antibodies of the invention, and the formed antigen-antibody complexes were detected with secondary anti-murinc antibodies conjugated with horse-radish peroxidase (hrp) (Figure 3). All tested anti-FOLRl antibodies recognized FOLRI in cell lines with high levels of FOLRI expression (i.e., Igrov-1 and Wish). FOLRI in low expressing cell lines Ovcar-3, Caov-3 and Skov-3 was detected only by anti-FOLRl clones 2.1 and 9.21; clones 3.8 and 5.7 did not stain, these cell lysates perhaps due to insufficient sensitivity of the antibodies. No additional non-specific bands were detected in FOLR1-positive cell lines by the clones; no staining of FOLRI-negative cell lines was observed.

&amp;#1470;θ2 -

Binding of the anti-FOLRl antibodies to denatured and not denatured cells

[0357] The ability of the anti-FOLRl antibodies to bind to denatured and nondenatured (native confirmation) FOLRI was assayed by indirect FACS with FOLRI-positive cells KB and T47D. Cells were harvested by Versine and washed with phosphate buffered saline (PBS). Denatured cells were prepared by incubation of the cells in PBS containing 10% formaldehyde at 4 °C overnight followed by washing with PBS and incubation at 95 °C for 30 min. Denatured and non-denatured cells were then incubated with anti-FOLRl antibodies diluted in FACS buffer (RPMI-1640 medium supplemented with 2% normal goat serum) on ice for 2 hours. The cells were centrifuged, washed with PBS and incubated for 40 min with FITC-conjugatcd goat anti-mouse IgG-antibody. The cells were centrifuged again, washed with PBS and resuspended with 0.2 ml of PBS containing 1% formaldehyde. Cellassociated fluorescence was measured using a FACSCalibur flow cytometer with the HTS multiwell and analyzed using CellQuest Pro (BD Biosciences, San Diego, US). As shown on Figure 4, all anti-FOLRl antibodies bound to both denatured and not denatured cells.

Affinity of the anti-FOLRl antibodies by ELISA

[0358] The binding affinity of the anti-FOLRl antibodies was examined by ELISA where recombinant humanFOLRl-murine Fc2a protein was used as the antigen. The recombinant protein was immobilized on microtiter plates, and the antibodies were added at a range of concentrations to the plates. The plates were incubated for two hours at room temperature, washed with PBS supplemented with 0.05% Tween-20, and incubated with hrplabeled goat anti-murine secondary antibody for one hour at room temperature. The plates were washed with PBS/Tween-20 again, and bound hrp-conjugated antibody was detected by adding the hrp-substrate TMB (Bio-FX). Representative results are shown in Figure 5. The anti-FOLRl antibodies had similar affinity to human FOLRI at half-maximal effective concentration (EC50) of 0.5 to 0.9 nM.

No cross-reactivity of the anti-FOLRl antibodies with FOLR2 and FOLR3

[0359] FOLRI is a member of Folate Receptor family. Cross-reactivity of the antiFOLRI antibodies with the other members of the family FOLR2 and FOLR3 was assayed by

ELISA. Recombinant protein FOLR2-His or FOLR3-His (R&amp;D Systems) was immobilized to Ni-NTA plates (QIAGEN) and the anti-FOLRl antibodies were added to the plates and incubated for 2 hours at room temperature. As positive controls for FOLR2 and FOLR3

ELISA polyclonal anti-FOLR2 and FOLR3 antibodies (R&amp;D systems), respectively, were

used. The formed antibody-antigen complexes were detected with hrp-labeled goat antimurine secondary antibody. As shown in Figure 6, the anti-FOLRl antibodies of the invention did not bind to FOLR2 or FOLR3; only the control antibodies detected corresponding antigens.

Example 4. Antigen epitope characterization

[0360] Human FOLRI has three potential sites for N-glycosylation at positions 69, 161 and 201 (UniProt), and, as reported in literature, all three sites are glycosylated. To characterize the nature of the epitopes recognized by the anti-FOLRl antibodies described herein, binding experiments were performed with deglycosylated and non-treated receptor. Of the generated anti-FOLRl clones, only clone 2.1. was used in the study because, based on the sequencing data, the clones are related and likely to bind to the same epitope. In addition to clone 2.1, two other anti-FOLRl antibodies were included: huM0v!9 (WO 2011/106528) and clone BN3.2 (Leica). In order to deglycosylate FOLRI, recombinant human FOLRI or lysates of FOLRI-positive KB or Igrov-1 cells were treated with a mixture of de glycosylation enzymes (Enzymatic DeGlycoMX Kit, QA-bio) according to the Manufacturer’s protocol. Then, samples of treated and non-treated FOLRI were used in ELISA and Western blot analysis. For the ELISA, deglycosylated and non-treated FOLRI were immobilized to ELISA plates (Immulon), and the anti-FOLRl antibodies FRIHC2-1 (2.1) or huM0vl9 were added. After 2 h incubation, antibody-antigen complexes were detected with hrp-labeled goat anti-human (for huM0vl9) or anti-murine (for 2.1) secondary antibody (Figure 7). For the Western blot analysis, samples of dcglycosylatcd and non-treated lysates or huFOLRl recombinant protein were separated by SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane by the standard procedures. The membrane was incubated with the anti-FOLRl antibodies 2.1, huM0vl9, or BN3.2, and the antigen-antibody complexes were detected with the appropriate secondary anti-murine or anti human antibodies conjugated with horse-radish peroxidase (Figure 8). As shown in Figures 7 and 8, binding of antibody 2.1 to deglycosylated vs. non-treated FOLRI was significantly reduced suggesting the antibody binds to a glycodependent epitope. In contrast, the other two antiFOLRI antibodies, huMovl9 and BN3.2, bind similarly to deglycosylated and non-treated receptor indicating that (i) the FOLRI protein was not damaged during the de glycosylation procedure and (ii) huM0vl9 and BN3.2 recognize protein epitopes of FOLRI.

Example 5. Cloning and sequencing of the VL and VH regions of the anti-human FOLRI antibodies

[0361] Total cellular RNA was prepared from 5 x 106 cells of the FOLRI hybridomas described in Example 1 using an RNeasy kit (QIAgen) according to the manufacturer’s protocol. cDNA for the eight subclones clones (2.1, 2.12, 3.8, 3.9, 5.7, 5.10, 9.20, and 9.21) was subsequently synthesized from total RNA using the SuperScript II cDNA synthesis kit (Invitrogen).

[0362] The PCR procedures for amplifying the antibody variable region cDNAs derived from hybridoma cells were based on methods described in Wang ct al. ((2000) J Immunol Methods. 233:167-77) and Co et al. ((1992) J Immunol. 148:1149-54). The variable light chain (VL) and variable heavy chain (VH) sequences were amplified by degenerate primers on the 5'end and either murine kappa or IgGl constant region specific primers respectively on the 3' end. The PCR reactions were then run on a 1% low melt agarose gel, followed by the excision of the 300 to 400 bp amplicon bands that were subsequently purified using Zymo DNA mini columns. The purified amplicons were sent to Beckman Coulter Genomics for sequencing utilizing the same 5' and 3' primers of the PCR reactions in order to generate the variable region cDNA sequences from both directions.

[0363] Since the degenerate primers used to clone the VL and VH cDNA sequences alter the 5' end, additional sequencing efforts were needed to verify the complete cDNA sequences. The preliminary sequences were entered into a search query of the NCBI IgBlast site (www.ncbi.nlm.nih.gov/igblast/) to identify the murine germline sequences from which the antibody sequences had been derived. PCR primers were then designed to anneal to the germline linked leader sequence of the murine antibody so that this new PCR reaction would yield a complete variable region cDNA sequence, unaltered by the PCR primers. The PCR reactions, band purifications, and sequencing were performed as described above.

Mass determination for sequence confirmation

[0364] The variable regions cDNA sequences obtained for each of the anti-FOLRl antibodies were combined with germline constant region sequences to obtain full length antibody cDNA sequences. The molecular weights of the heavy and light chains were then calculated from translations of the cDNA sequences and compared &amp;#1470;with the molecular weights obtained by LC/MS analyses of the purified murine anti-FOLRl antibodies. The

LC/MS is done by deglycosylating and reducing the antibody to isolate full chain light and heavy chain peptides. The observed molecular weights for each of the heavy chains matched

the expected, but each of the light chains was off by approximately 85 Da, Subsequent peptide fragmentation analysis by LC/MS of the light chain fragments indicated that the final serine of light chain leader peptide was in fact retained on the mature light chain, adding about 87 Da to the expected MW, thus confirming the cDNA sequences for each of the FOLRI antibodies.

Composite CDR sequences for the anti-FOLRl antibodies

[0365] Alignments of the antibody sequences for the 8 subclones revealed that 3 of the 4 original hybridomas had produced closely related, but unique, antibodies. As expected, each of the 4 sister subclone pairs were identical. In addition two sets of subclones were also identical resulting in the 3 unique antibody sequences (SEQ ID NOs:27-32) (2.1, 5.7, and 9.20). The light and heavy chain variable framework sequences of these 3 unique antibodies are closely related, but each antibody contains unique CDRs, likely as a result of somatic amino acid substitutions (see Table 14 below). Because these CDR variants of the murine anti-FOLRl antibodies were found to be functionally identical, they provide some structural insight into the sequence flexibility of the CDRs of the anti-FOLRl antibodies of the invention. Light chain CDRs 2 and 3 were identical in each of the antibodies suggesting that these tightly conserved CDRs can provide a consistent structural basis for FOLRI binding. On the other hand, the amino acid substitutions in the remaining CDRs, particularly those in heavy chain CDRs 2 and 3, suggest that these positions are critical for refinement of the affinity and specificity of these antibodies. The specific residue substitutions in these CDR positions also provide examples of residues that can be incorporated within engineered versions of these antibodies. Table 14 provides a composite CDR sequence listing compiled from the anti-FOLRl antibodies of the invention. The composite CDRs identified herein can be used for the design of recombinant antibodies that would be expected to preserve the functional attributes of the anti-FOLRl antibodies of the present invention.

Table 14 Composite CDRS

Anti-FOLRl composite CDRs

Light Chain

CDRl: KS[T/S][K/E]SLLNSDGFTYLD (SEQ ID NO:24)

CDR2: LVSNHFS (SEQ ID NO:25)

CDR3 . FQSNYLPLT (SEQ ID NO:26)

Heavy Chain

&amp;#1470;

CDRl: N[Y/S]YIH7SEQ ID NO:21)

CDR2: WIYP[G/E][S/N][F/V/L]N[V/T][E/R/Q]YN[E/D]KFKA (SEQ ID NO :22)

CDR3: RGIY[F/Y]YSPYA[L/M]D[Y/H] (SEQ ID NO:23)

Antibody humanization

[0366] The FRIHC2-1 antibody was humanized following resurfacing methods previously described, such as, for example in Roguska et al., Proc. Natl. Acad. Sci., USA, 91(3):969-973 (1994) and Roguska et al., Protein Eng. 9(10):895-904 (1996), which are incorporated in their entirety herein by reference. Resurfacing involves identification of the variable region framework surface residues in both the light and heavy chains and replacing them with human equivalents. The murine CDRs are preserved in the resurfaced antibody. Exemplary CDRs of FRIHC2-1 antibody are defined as indicated in Table 14. To minimize concerns about the impact of conjugating lysines that fall in CDRs, lysine 24 and lysine 27 in murine FRIHC2-1 antibody light chain CDRl were replaced with arginine for humanized version 1.0 (shown in italic), so both versions of the LC CDRl are given. In addition to the AbM heavy chain CDR2 definition employed for resurfacing, the table provides exemplary Kabat defined heavy chain CDR2s for both the murine and human versions of FR1HC2-1 antibody. The underlined sequence marks the portion of the Kabat heavy chain CDR2 that was not considered a CDR for resurfacing.

[0367] Surface residue positions were defined as any position with a relative accessibility of 30% or greater (Pedersen J.T. et. Al, J. Mol. Biol. 1994; 235: 959-973). The calculated surface residues were then aligned with human gcrmline surface sequences to identify the most homologous human surface sequence. The human gcrmline sequence used as the replacement surface for the VL domains of FRIHC2-1 antibody was 10Κν2-30*01 while ΙΗν1-69*10 was used as the replacement surface for FRJHC2-1 antibody VH. The specific framework surface residue changes for FRIHC2-1 antibody are given in Figure 9. Since the resurfaced light chain included the CDRl lysine substitutions in the preferred version, a resurfaced version (vl.01) was also generated with murine lysines retained in CDR-L1, Figure 10 shows the alignment of the resurfaced sequences for FRIHC2-1 variable domain of both light and heavy chain with their murine counterparts.

[0368] In addition to humanization by variable domain resurfacing, FRIHC2-1 antibody was also humanized following complementary determining region (CDR) grafting technology (Jones et al., Nature 321: 604-608 (1986) and Verhoeyen et al., Science 239:

WO 2015/031815 &amp;#1470; 107 &amp;#1470;

1534-1536 (1988)). The CDR grafting method consists of grafting the CDRs from a naturally evolved murine antibody onto the Fv framework regions (FRs) of a human antibody. The Kabat numbering scheme and Kabat CDR definitions were used for CDR grafting of the FRIHC2-1 antibody. Exemplary CDRs of FRIHC2-1 for CDR grafting are given in Table 14. The human immunoglobulin germline sequence with the highest homology to the murine FRIHC2-1 antibody was identified through the interactive tool, VQUEST, of the International ImMunoGeneTics information system® (IMGT (http://imgt.cines.fr/) as described in Lefranc, Nucleic Acids Res. 29: 207-209 (2001). The human germline sequences used as the acceptor frameworks for the VL and VH domains of FRIHC2-1 antibody were IGKV2D-29*02 and IGHV 1-2*02, respectively. To minimize concerns about the impact of conjugating lysines that fall in CDRs, lysine 24 and lysine 27 in murine FRIHC2-1 antibody light chain CDR1 were replaced with arginine in the CDR grafted constructs (Table 15). The specific framework residue changes as well as the substitution in CDR-L1 in CDR-grafting of FRIHC2-1 antibody are given in Figure 11, and the alignments of the CDR-grafted sequences for the FRIHC2-1 antibody variable domains with its murine counterparts are illustrated in Figure 12.

Table 15

<td> FRIHC2-1 CDR’s (Resurfacing)</td><td> FRIHC2-1 CDR’s (CDR grafting)</td>

<td> Light Chain</td><td> Light Chain</td>

<td> Murine and resurfaced vl.01 CDR1: KSSKSLLNSDGFTYLD (SEQ ID NO:6) Resurfaced vl.O CDR1: RSS7?SLLNSDGFTYLD (SEQ ID NO:59)</td><td> Murine and CDR-grafted vl.01 CDR1: KSSKSLLNSDGFTYLD (SEQ ID NO:6) CDR-grafted vl.O CDR1: RSSRSLLNSDGFTYLD (SEQ ID NO:59)</td>

<td> CDR2: LVSNHFS (SEQ ID NO:7)</td><td> CDR2: LVSNHFS (SEQ ID NO:7)</td>

<td> CDR3: FQSNYLPLT (SEQ ID NO:8)</td><td> CDR3: FQSNYLPLT (SEQ ID NO :8)</td>

<td> Heavy Chain</td><td> Heavy Chain</td>

<td> CDR1: NSYIH (SEQ ID NO:3)</td><td> CDR1: NSYIH (SEQ ID NO:3)</td>

<td> CDR2: WIYPESLNTQ (SEQ ID NO:60)</td><td> CDR2: WIYPESLNTQYNEKFKA (SEQ ID NO:4)</td>

<td> CDR3: RG1YYYSPYALDH (SEQ ID NO:5)</td><td> CDR3: RGIYYYSPYALDH (SEQ ID NO:5)</td>

<td> Kabat FRIHC2-1 HC CDR2</td><td></td>

<td> Murine HC CDR2: WIYPESLNTQYNEKFKA (SEQ ID NO :4)</td><td></td>

WO 2015/031815 &amp;#1470; 108 &amp;#1470;

<td> Resurfaced HC CDR2: WIYPESLNTQYNQKFQG (SEQ ID NO:61)</td><td></td>

Example 6. IHC evaluation of 353-2.1 (FOLR1-2.1) antibody using human tumor samples.

[0369] Human tumor samples representative of ovarian cancer (n=63), lung adenocarcinoma (n=104), and endometrial adenocarcinoma (n=58) were evaluated for FOLRI expression by IHC using the 353-2.1 antibody. The intensity of FOLRI staining and the distribution of scores are summarized in Table 16, below. Figure 15 shows an example of staining of ovarian cancer and lung adenocarcinoma tissue with the 353-2.1 antibody. These results demonstrate the utility of 353-2.1 as a specific and sensitive antibody for use in IHC assays to identify patients as potential candidates for therapy with FOLRI targeting agents (e.g., IMGN853).

Table 16: Distribution of Scores (% Positivity)

<td> TUMOR TYPE:</td><td> OVARIAN CANCER 11=63</td><td> LUNG ADENOCARCINOMA n=104</td><td> ENDOMETRIAL ADENOCARCINOMA n=58</td>

<td> Positive (any intensity):</td><td> 65%</td><td> 70%</td><td> 64%</td>

<td> &gt; level 2 intensity with at least 25% tumor cells stained:</td><td> 59%</td><td> 47%</td><td> 33%</td>

<td> &gt;level 3 intensity with at least 25% tumor cells stained:</td><td> 51%</td><td> 19%</td><td> 14%</td>

[0370] The unique antigen specificity and high binding affinity of the FOLRI-2.1 (FOLRI 353-2.1) antibody was further demonstrated using an additional IHC assay. This IHC assay utilizes the OptiView DAB Detection Kit on a Ventana BenchMark XT automated slide Stainer for semi-quantitative determination of FOLRI protein expression in formalinfixed paraffin embedded tissue samples. The assay has been optimized and validated with respect to specificity, sensitivity, and precision using normal and. tumor tissue controls. Under the optimized condition, sharp membranous staining was clearly observed in tumor cellss whereas normal stromal tissues were completely negative. (Figure 16). In addition, this assay also achieved a broader dynamic range thereby allowing better discrimination of moderate staining intensity (level 2, medium brown staining, Figure 17) from the strongest staining intensity (level 3, dark brown staining, Figure 17). The enhanced dynamic range improves the

ability to rank FOLRI positive samples based on staining intensity and enable further identification a sub-popupation of patients with the highest level of FOLRI expression.

[0371] A BN3.2 (Leica) antibody and the FOLRI-2.1 (FOLRI 353-2.1) antibody were compared using an ovarian cancer tissue micro array (TMA). Using the BN3.2 (Leica) antibody in an IHC assay (BN3.2 assay), close to 50% of samples (16 out of 35) were scored in the highest category (level 3 staining intensity on at least 25% tumor cells). In contrast, using the FOLRI-2.1 (FOLRI 3532.1&amp;#1470;) antibody in the IHC assay described above utilizing the OptiView DAB Detection Kit on a Ventana BenchMark XT automated slide stainer (FOLRI-2.1 assay), allowed futher separation of these 16 samples into 2 different categories: 6 in the highest category (level 3 staining intensity on at least 25% tumor cells, Table 17), and the other 10 in the second highest category (level 2 staining intensity on at least 25% tumor cells, Table 17). Thus, the more discreet staining obtained with the FOLRI-2.1 antibody in the FOLRI-2.1 assay allows for discrimination among samples all grouped together as samples with level 3 expression using the BN3.2 antibody in the BN3.2 assay.

Table 17: FOLRI prevalence comparison in ovarian cancer TMA (n=35)

<td> Score</td><td> FOLRI-2.1 assay</td><td> BN3.2 assay</td>

<td> Positive (any intensity)</td><td> 24 (69%)</td><td> 28 (80%)</td>

<td> &gt; level 1 intensity with at least 25% tumor cells stained:</td><td> 21 (60%)</td><td> 27 (77%)</td>

<td> &gt; level 2 intensity with at least 25% tumor cells stained:</td><td> 17 (49%)</td><td> 25 (71%)</td>

<td> &gt; level 3 intensity with at least 25% tumor cells stained:</td><td> 6 (17%)</td><td> 16(46%)</td>

[0372] All publications, patents, patent applications, internet sites, and accession numbers/databasc sequences (including both polynucleotide and polypeptide sequences) cited herein are hereby incorporated by reference in their entirety for all purposes to the same extent as if each individual publication, patent, patent application, internet site, or accession

number/database sequence were specifically and individually indicated to be so incorporated by reference.

PCI7US2014/053512

NSCLC Adenocarcinoma (20x) Ovarian Endometroid Adenocarcinoma (20x)

<img file="IL293871A_D0001.tif" />

<img file="IL293871A_D0002.tif" />

Normal Salivary Gland (20x) Normal Pancreas (20x)

<img file="IL293871A_D0003.tif" />

<img file="IL293871A_D0004.tif" />

FIG. 2

Clone 9.21 Clone 2.1 Clone 3.8 Clone 5.7

<img file="IL293871A_D0005.tif" />

CO &#1507; N CXI cxi co io &#1504;&#1505; > © Φ B

<img file="IL293871A_D0006.tif" />

FIG. 4A s|!up luoosojonij &#1498;&#1490; CO S C\! cm co ι&#972; σ>

<img file="IL293871A_D0007.tif" />

FIG.4B s!iup |uoosojon|j

<img file="IL293871A_D0008.tif" />

<img file="IL293871A_D0009.tif" />

<img file="IL293871A_D0010.tif" />

WU0917 &amp;#9633;Ο

F0LR2-ELISA

<img file="IL293871A_D0011.tif" />

FIG. 6A wuostz ao

<img file="IL293871A_D0012.tif" />

FIG. 6B

LUUOGtz GO wuost ao

<img file="IL293871A_D0013.tif" />

huM0v19, binding to non treated FOLR1 x huMov19, bindnig to non treated FOLR1 incubated in deglycosylation buffer (no deglycosylation enzymes added) &amp;#9633; huM0v19, binding to deglycosylated FOLR1 in deglycosylation buffer

2.1, binding to non treated FOLR1

2.1 &amp;#1488;, binding to non treated FOLR1 incubated in deglycosylation buffer (no deglycosylation enzymes added) &amp;#9633; 2.1, binding to deglycosylated FOLR1 in deglycosylation buffer

FIG. 7

<img file="IL293871A_D0014.tif" />

FIG. 8

Resurfacing of FRIHC2-1

<td colspan="4"> FRIHC2-1- V_L</td>

<td> Kabat position</td><td> Murine residue</td><td> Human v1.0 residue</td><td> Human v1.01 residue</td>

<td> 1</td><td> D</td><td> D</td><td> D</td>

<td> 3</td><td> V</td><td> V</td><td> V</td>

<td> 7</td><td> T</td><td> s</td><td> s</td>

<td> 9</td><td> L</td><td> L</td><td> L</td>

<td> 15</td><td> I</td><td> L</td><td> L</td>

<td> 17</td><td> D</td><td> P</td><td> Q.</td>

<td> 18</td><td> Q</td><td> P</td><td> P</td>

<td> 40</td><td> P</td><td> P</td><td> P</td>

<td> 41</td><td> G</td><td> G</td><td> G</td>

<td> 42</td><td> K</td><td> K</td><td> K</td>

<td> 45</td><td> Q</td><td> R</td><td> R</td>

<td> 57</td><td> G</td><td> G</td><td> G</td>

<td> 60</td><td> D</td><td> D</td><td> D</td>

<td> 67</td><td> S</td><td> S</td><td> s</td>

<td> 77</td><td> R</td><td> R</td><td> R</td>

<td> 81</td><td> E</td><td> E</td><td> E</td>

<td> 100</td><td> G</td><td> P</td><td> P</td>

<td> 103</td><td> K</td><td> K</td><td> K</td>

<td> 107</td><td> K</td><td> K</td><td> K</td>

<td> 108</td><td> R</td><td> R</td><td> R</td>

<td> 24</td><td> K</td><td> R</td><td> K</td>

<td> 27</td><td> K</td><td> R</td><td> K</td>

<td colspan="3"> FRIHC2-1- V_H</td>

<td> Kabat position</td><td> Murine residue</td><td> Human residue</td>

<td> 1</td><td> Q</td><td> Q</td>

<td> 3</td><td> Q</td><td> Q</td>

<td> 5</td><td> Q</td><td> y</td>

<td> 9</td><td> P</td><td> A</td>

<td> 11</td><td> L</td><td> X</td>

<td> 13</td><td> K</td><td> K</td>

<td> 14</td><td> P</td><td> P</td>

<td> 19</td><td> R</td><td> K</td>

<td> 23</td><td> K</td><td> K</td>

<td> 28</td><td> T</td><td> T</td>

<td> 41</td><td> P</td><td> P</td>

<td> 42</td><td> G</td><td> G</td>

<td> 43</td><td> Q</td><td> Q</td>

<td> 61</td><td> E</td><td> P</td>

<td> 62</td><td> K</td><td> K</td>

<td> 64</td><td> K</td><td> P</td>

<td> 65</td><td> A</td><td> G</td>

<td> 73</td><td> K</td><td> K</td>

<td> 74</td><td> s</td><td> s</td>

<td> 82b</td><td> s</td><td> s</td>

<td> 84</td><td> s</td><td> s</td>

<td> 85</td><td> E</td><td> E</td>

<td> 105</td><td> Q</td><td> Q</td>

<td> 112</td><td> S</td><td> S</td>

FIG. 9

<img file="IL293871A_D0015.tif" />

Resurfacing alignments i 1

H 1 &amp;#943;

<img file="IL293871A_D0016.tif" />

S &amp;#912; ' '&amp;#943;^ S tI u &amp;#1503;&amp;#943; &amp; 11 &amp;#970;&amp;#1494;

I 1

<img file="IL293871A_D0017.tif" />

&amp;#943; »η! 5$ &amp;#974; ?Μ &amp;#912; &amp;#943;§|

<img file="IL293871A_D0018.tif" />

esi

CD ½¾:

<img file="IL293871A_D0019.tif" />

CDR-grafting of FRIHC2-1

<td colspan="4"> FRIHC2-1-V,</td>

<td> Kabat position</td><td> Murine residue</td><td> Human (CDRgraft) v1.0 residue</td><td> Human (CDRgraft) v1.01 residue</td>

<td> 2</td><td> V</td><td> [</td><td> I</td>

<td> 4</td><td> L</td><td> M</td><td> M</td>

<td> 12</td><td> P</td><td> S</td><td> S</td>

<td> 14</td><td> N</td><td> T</td><td> T</td>

<td> 15</td><td> [</td><td> P</td><td> P</td>

<td> 17</td><td> D</td><td> Q</td><td> Q</td>

<td> 18</td><td> Q</td><td> P</td><td> P</td>

<td> 83</td><td> L</td><td> V</td><td> V</td>

<td> 100</td><td> G</td><td> Q</td><td> Q</td>

<td> 24</td><td> K</td><td> R</td><td> K</td>

<td> 27</td><td> K</td><td> R</td><td> K</td>

<td colspan="3"> FRiHC2-1-V_H</td>

<td> Kabat position</td><td> Murine residue</td><td> Human (CDR-graft) residue</td>

<td> 5</td><td> Q</td><td> V</td>

<td> 9</td><td> P</td><td> A</td>

<td> 11</td><td> L</td><td> V</td>

<td> 12</td><td> V</td><td> K</td>

<td> 19</td><td> R</td><td> K</td>

<td> 20</td><td> 1</td><td> V</td>

<td> 38</td><td> K</td><td> R</td>

<td> 39</td><td> K</td><td> Q</td>

<td> 40</td><td> R</td><td> A</td>

<td> 48</td><td> 1</td><td> M</td>

<td> 66</td><td> K</td><td> R</td>

<td> 67</td><td> A</td><td> V</td>

<td> 69</td><td> L</td><td> M</td>

<td> 71</td><td> A</td><td> R</td>

<td> 73</td><td> K</td><td> T</td>

<td> 75</td><td> S</td><td> I</td>

<td> 78</td><td> s</td><td> A</td>

<td> 81</td><td> Q</td><td> E</td>

<td> 82b</td><td> s</td><td> R</td>

<td> 83</td><td> T</td><td> R</td>

<td> 85</td><td> E</td><td> D</td>

<td> 87</td><td> s</td><td> T</td>

<td> 91</td><td> F</td><td> Y</td>

<td> 107</td><td> A</td><td> T</td>

<td> 108</td><td> S</td><td> L</td>

FIG. 11

<img file="IL293871A_D0020.tif" />

<img file="IL293871A_D0021.tif" />

<img file="IL293871A_D0022.tif" />

<img file="IL293871A_D0023.tif" />

NX 1

I &amp;#942;: t

<img file="IL293871A_D0024.tif" />

<img file="IL293871A_D0025.tif" />

FIG. 12

<img file="IL293871A_D0026.tif" />

<img file="IL293871A_D0027.tif" />

<img file="IL293871A_D0028.tif" />

<img file="IL293871A_D0029.tif" />

<img file="IL293871A_D0030.tif" />

<img file="IL293871A_D0031.tif" />

CQ io

&amp;#1496;

<img file="IL293871A_D0032.tif" />

<img file="IL293871A_D0033.tif" />

&amp;#1470;- 4/053512