Proteins for preparation of medicaments for treatment of hematological malignancies

225488/3

PROTEINS FOR PREPARATION OF MEDICAMENTS FOR TREATMENT

OC IIIP1ΑΊΌΙ .OGIAND MALIGNANCES

FIELD OF THE INVENTION 5 Lhis invention relates to compositions and methods for treatment of hematological malignancies, and in particular, but not exclusively, to fusion proteins and compositions and methods of use thereof.

BACKGROUND OF THE INVENTION

Lhere are two major arms to the immune system, supported by different 10 types of cells called B-lymphocytes and L-lymphocytes CB-cells and L-cells). B cells make antibodies when they encounter antigens and, in most instances, these antibodies are protective. In autoimmune diseases, however, some of the antibodies react with the individual's tissues. When they deposit in tissue, they cause an inflammatory reaction and tissue damage. L-cells, like B-cells, are 15 also activated when they encounter an antigen. As L-cells develop they undergo a process called "thymic education." During thymic education, more than 95% of the L-cells die. Lhe L-cells that have had a L-cell receptor that can recognize and react with the individuals's own tissues (self-antigens) are specifically eliminated. Some autoreactive L-cells escape the elimination 20 process, however, and can initiate an immune response that results in autoimmune disease.

Lhe modulation of L-cell activity remains a significant therapeutic goal in diseases with immunopathological L-cells. Lhe fate of L lymphocytes following L cell receptor (LCR) stimulation is guided by the integration of 25 costimulatory and inhibitory receptor inputs. Costimulatory ligands on antigen- presenting cells (APC) trigger cognate receptor molecules on L cells, with resultant enhancement of L cell proliferation, cytokine secretion, and differentiation. In contrast, binding of inhibitory ligand molecules to cognate 1 225488/3 counter-receptors on T lymphocytes diminishes effector functioning by inducing T cell unresponsiveness or programmed cell death (PCD) (also referred to as apoptosis). Costimulatory and inhibitory receptor pathway interactions are suggested by experiments demonstrating increased inhibitor 5 activity in the presence of costimulator blockade.

Cytotoxic T lymphocyte-associated protein-4 (CTLA-4 (CD152)) is an inhibitory receptor molecule that is expressed on the surface of activated T lymphocytes. Following engagement with the B7-1 (CD80) and/or B7-2 (CD86) ligands resident on APC, the CTLA-4 counter-receptor, via associated 10 SHP-2 phosphatase, inhibits T cell activation. On activated T cells, CTLA-4 exists as disulfide-linked homodimeric glycoprotein complexes. A recombinant, soluble CTLA-4:immunoglobulin G (CTLA-4:Ig) chimeric protein demonstrates inhibitory function by competitively blocking CD80/CD86 molecule binding to the activating CD28 acceptor on T cell 15 surfaces. CTLA-4:Ig also exhibits immunosuppressive activity in animal models of graft rejection and autoimmune disease by blocking T cell costimulation through CD28. In addition, intracellular T cell survival signaling through CD28 is antagonized by APC treatment with CTLA-4:Ig, which can increase susceptibility to Fas-dependent PCD. The action of CTLA-4, as well 20 as CTLA-4:Ig fusion proteins, are discussed in U.S. Pat. Nos. 5,885,776; 5,885,579; 5,851,795; and 5,968,510.

Apoptosis (or PCD) is a distinct form of cell death which is essential for the regulation of cellular homeostasis. In the immune system, Fas (CD95) receptor and its ligand, FasL (CD95L), participate in various processes 25 involved in the induction of apoptosis, including immune cell-mediated cytotoxicity, and in the regulation of cellular immune responses. FasL is a member of the tumor necrosis factor superfamily and is expressed by a restricted subset of immune cells, including monocytes, NK cells, and activated B and T cells. On the cell surface, FasL is oriented as a type II membrane 30 protein within trimeric complexes. Metalloproteinase cleavage of membrane- 2 225488/3 associated FasL releases soluble FasL (sFasL) trimers from the membrane. The FasL molecule triggers Fas-dependent PCD.

The valency of a molecule or molecular complex can be increased by association with the cell surface. Different coding sequences of recombinant sFasL molecules affect macromolecular aggregation and, in turn, affect sFasL pro-apoptotic function. In particular, a naturally processed sFasL molecule forms trimers and poorly induces apoptosis. In contrast, a recombinant full length extracellular domain sFasL polypeptide forms higher order aggregates and displays highly potent apoptotic activity. Furthermore, complexes of sFasL produced by recombinant expression in human 293 cells require cross-linking for lysis of Fas-sensitive cells. U.S. Pat. No. 5,830,469 discloses monoclonal antibodies and binding proteins that specifically bind to human Fas antigen; some of the antigens and antibodies are reported as stimulating T cell proliferation, inhibiting of anti-Fas CH-11 monoclonal antibody-mediated lysis of cells, and blocking Fas ligand mediated lysis of cells. Fas-Fe fusion proteins are also disclosed. U.S. Pat. Nos. 5,242,687; 5,601,828; and 5,623,056 disclose various fusion proteins containing a CD8 component that bind to a cell but do not mask a signal produced by the cell. U.S. Pat. No. 5,359,046 discloses chimeric proteins comprised of an extracellular domain capable of binding to a ligand in a non-MHC restricted manner, a transmembrane domain and a cytoplasmic domain capable of activating a signaling pathway. Similar technology is disclosed in U.S. Pat. No. 5,686,281. US 2003/216546 discloses chimeric proteins, comprising at least two portions, wherein the first portion binds to a first cell and decreases the cell's ability to send a trans signal to a second cell; the second portion sends its own trans signal to the second cell. US 2003/216546 further discloses in which two molecular components result in decrement of a first trans signal from a first cell and the conferring of a second trans signal to a second cell.

Shi W. et al., (Graefe's Archive for Clinical and Experimental Ophthalmology, 2007, 245(11), 1691-1697) discloses investigation of the 3 225488/3 therapeutic effect of CTLA4-FasL-B7 costimulatory pathway blockage on graft survival in a murine model of corneal transportation.

Dranitzki-Elhalel, M. et al., (Cellular immunology, 2006, 239(2), 129135) discloses that CTl.A-44;as ligand (CTl.A-44;asl.), a paradigmatic '“trans 5 signal converter protein (TSCP)”, can attach to APC (via CTLA-4 binding to B7) and direct intercellular inhibitory signals to responding T cells (via Fasl binding to Fas receptor), converting an activating APC-to-T cell signal into an inhibitory one.

Orbach, A. et al., (The Journal of Immunology, 2007, 179(11), 728710 7294) investigates CTlA-4 Fasl's mechanism of action and suggest a model for CTlA-4 Fasl action wherein there is coordinate triggering of a death receptor and suppression of a proapoptotic protein. 3a 225488/3

SUMMARY OF THE INVENTION

The background art does not teach or suggest methods of treatment of lymphoma and multiple myeloma through the administration of chimeric proteins which are useful for both blocking and signaling. 5 The present invention, in at least some embodiments, overcomes these drawbacks of the background art by providing methods of treatment of lymphoma and/or multiple myeloma through the administration of chimeric proteins which are useful for both blocking and signaling.

In at least some embodiments, there is provided pharmaceutical 10 compositions containing such chimeric proteins, adapted for treatment of lymphoma and/or multiple myeloma.

By "lymphoma" it is meant a malignant growth of B or T cells in the lymphatic system, optionally including Hodgkin's lymphoma or non-Hodgkin's lymphoma (NHL). 15 According to at least some embodiments, the non-Hodgkin's Lymphoma is a selected from the group consisting of aggressive NHL, transformed NHL, indolent NHL, relapsed NHL, refractory NHL, low grade non-Hodgkin's Lymphoma, follicular lymphoma, large cell lymphoma, B-cell lymphoma, T-cell lymphoma, Mantle cell lymphoma, Burkitt's lymphoma, NK cell 20 lymphoma, diffuse large B-cell lymphoma, acute lymphoblastic lymphoma, and cutaneous T cell cancer, including mycosos fungoides/Sezry syndrome.

An "indolent" non-Hodgkin's Lymphoma is a classification that includes slow growing forms of lymphoma. They encompass what are called low grade and some categories of intermediate grade NHL in the Working Formulation. 25 Indolent NHLs are sometimes not responsive to conventional cancer therapies such as chemotherapy and radiation therapy. Indolent NHL and other premalignant forms of NHL may also proceed to NHL. With regard to premalignant or benign forms of the disease, optionally the compositions and 4 225488/3 methods thereof may be applied for prevention, in addition to or in place of treatment, for example optionally to halt the progression of the disease to a malignant form of NHL. A "transformed" non-Hodgkin's Lymphoma is a classification sometimes 5 employed to describe an indolent NHL which acquires an aggressive aspect and becomes more responsive to standard chemotherapies.

By "multiple myeloma" it is meant any type of B-cell malignancy characterized by the accumulation of terminally differentiated B-cells (plasma cells) in the bone marrow. 10 According to at least some embodiments, the multiple myeloma is

selected from the group consisting of multiple myeloma cancers which produce light chains of kappa-type and/or light chains of lambda-type; and/or aggressive multiple myeloma, including primary plasma cell leukemia (PCL); and/or optionally including benign plasma cell disorders such as MGUS 15 (monoclonal gammopathy of undetermined significance) and/or Waldenstrom's macroglobulinemia (WM, also known as lymphoplasmacytic lymphoma) which may proceed to multiple myeloma; and/or smoldering multiple myeloma (SMM), and/or indolent multiple myeloma, premalignant forms of multiple myeloma which may also proceed to multiple myeloma; and/or primary 20 amyloidosis. With regard to premalignant or benign forms of the disease, optionally the compositions and methods thereof may be applied for prevention, in addition to or in place of treatment, for example optionally to halt the progression of the disease to a malignant form of multiple myeloma.

According to at least some embodiments of the present invention, there is 25 provided a method of treatment of a leukemia selected from the group consisting of acute nonlymphocytic leukemia, chronic lymphocytic leukemia, acute granulocytic leukemia, chronic granulocytic leukemia, acute promyelocytic leukemia, adult T-cell leukemia, aleukemic leukemia, a leukocythemic leukemia, basophylic leukemia, blast cell leukemia, bovine 5 225488/3 leukemia, chronic myelocytic leukemia, leukemia cutis, embryonal leukemia, eosinophilic leukemia, Gross' leukemia, hairy-cell leukemia, hemoblastic leukemia, hemocytoblastic leukemia, histiocytic leukemia, stem cell leukemia, acute monocytic leukemia, leukopenic leukemia, lymphatic leukemia, 5 lymphoblastic leukemia, lymphocytic leukemia, lymphogenous leukemia, lymphoid leukemia, lymphosarcoma cell leukemia, mast cell leukemia, megakaryocytic leukemia, micromyeloblastic leukemia, monocytic leukemia, myeloblastic leukemia, myelocytic leukemia, myeloid granulocytic leukemia, myelomonocytic leukemia, Naegeli leukemia, plasma cell leukemia, 10 plasmacytic leukemia, promyelocytic leukemia, Rieder cell leukemia,

Schilling's leukemia, stem cell leukemia, subleukemic leukemia, and undifferentiated cell leukemia.

In at least some embodiments, there is provided pharmaceutical compositions containing such chimeric proteins, adapted for treatment of a 15 leukemia selected from the above group.

According to at least some embodiments of the present invention, the chimeric protein is selected from the group consisting of CTLA4-FasL and CD40-FasL proteins. US Patent No. 7,569,663 to Tykocinski, et al., issued on August 4 2009, 20 describes chimeric proteins that function as both a blocking protein and a signaling protein. Such chimeric proteins include CTLA4-FasL and CD40-FasL proteins. Data was also provided in this patent that demonstrates the efficacy of the CTLA-4-FasL chimeric protein to inhibit the polyclonal proliferation of human peripheral blood T cells to mitogenic anti-CD3 antibody. Methods of use 25 and of production are also described.

As used herein the term "treatment" refers to care provided to relieve illness and refers to both a therapeutic treatment or prophylactic/preventative measures, wherein the objective is to prevent or slow down (lessen) the 6 225488/3 targeted pathologic condition or disorder. Those in need of treatment include those already with the disorder as well as those prone to have the disorder or those in whom the disorder is to be prevented. The term treatment as used herein refers also to “maintenance therapy”, which is a treatment that is given 5 to keep a pathologic condition or disorder from coming back after it has disappeared following the initial therapy.

The term "therapeutically effective amount" refers to an amount of agent according to the present invention that is effective to treat a disease or disorder in a mammal. 10 According to any of the above described embodiments of chimeric proteins and/or pharmaceutical compositions thereof, alone or in combination with other therapeutics or drugs (for combination therapy), there is provided, according to at least some embodiments of the present invention, compositions and methods of treatment therefore for treatment of a hematological 15 malignancy as described herein. Such one or more additional therapeutics or drugs could easily be selected by one of ordinary skill in the art.

As used herein the term "combination therapy" refers to the simultaneous or consecutive administration of two or more medications or types of therapy to treat a single disease, preferably with a synergistic effect. In particular, the term 20 refers to the use of any of the chimeric proteins or pharmaceutical compositions according to at least some embodiments of the invention in combination with at least one additional medication or therapy. Thus, treatment of a disease using the agents according to at least some embodiments of the present invention may be combined with therapies well known in the art that include, but are not 25 limited to, radiation therapy, antibody therapy, chemotherapy or surgery or in combination therapy with other biological agents, conventional drugs, anticancer agents, immunosuppressants, cytotoxic drugs for cancer, chemotherapeutic agents. 7 225488/3

According to at least some embodiments, treatment of Multiple Myeloma using the agents according to at least some embodiments of the present invention may be combined with an agent including but not limited to Melphalan, thalidomide (MPT), or combination Bortezomib (Velcade), 5 melphalan, prednisone (VMP) or a combination of Lenalidomide plus low-dose dexamethasone; and/or biophosphonates; chemotherapy (e.g., alkylating agents, vincristine, doxorubicin); autologous stem cell transplantation; and corticosteroids (e.g., prednisone and dexamethasone).

According to at least some embodiments, treatment of leukemia using the 10 agents according to at least some embodiments of the present invention may be combined with an agent including but not limited to alpha-interferon; interleukin-2; cytarabine and mitoxantrone; cytarabine and daunorubicin and 6-thioguanine; cyclophosphamide and 2-chloro-2'-deoxyadenosine; VP-16 and cytarabine and idorubicin or mitoxantrone; fludarabine and cytarabine and 15 .gamma.-CSF; chlorambucil; cyclophosphamide and vincristine and (prednisolone or prednisone) and optionally doxorubicin; tyrosine kinase inhibitor; and antibody; glutamine; clofibric acid; all-trans retinoic acid; ginseng diyne analog; KRN8602 (anthracycline drug); temozolomide and poly(ADP-ribose) polymerase inhibitors; lysofylline; cytosine arabinoside; 20 chlythorax and elemental enteral diet enriched with medium-chain triglycerides; amifostine; and gilvusmycin.

According to at least some embodiments, treatment of lymphoma using the agents according to at least some embodiments of the present invention may be combined with an agent including but not limited to a vinca alkaloid, 25 such as vincristine, vinblastine, vindesine, or vinorelbine; an anthracycline such as doxorubicin; combinations such as CHOP (vincristine, cyclophosphamide, doxorubicin and prednisone); and other suitable alkaloids including, but not limited to, the podophyllins, podophyllotoxins, and derivatives thereof (e.g., etoposide, etoposide phosphate, teniposide, etc.), the camptothecins (e.g., 30 irinotecan, topotecan, etc.) the taxanes (taxol, etc.), and derivatives thereof.. 8 225488/3

As used herein, the term "synergistic effect" or "synergism" refers to a greater effect seen with a combination of a plurality of therapeutic agents, including at least one therapeutic agent according to any embodiment of the present invention, in which the therapeutic effect is greater than the additive 5 effects of the plurality of agents when administered singly. By "greater therapeutic effect", it is meant a greater cancer effect and/or a reduction in one or more side effects.

As used herein, the term "subject" includes any human or nonhuman animal. The term "nonhuman animal" includes all vertebrates, e.g., mammals 10 and non-mammals, such as nonhuman primates, sheep, dogs, cats, horses, cows, chickens, amphibians, reptiles, etc.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1. Expression of fusion protein counter-receptors on T and B 15 malignant cell lines. CD40L-expressing (J-CD40L+) and non-expressing (JCD40L-) malignant T cell lines, as well as Raji and Daudi malignant B cell lines, were immuno-stained with FITC-conjugated anti-CD95, anti-CD80, anti-CD86 or anti-CD40L mAb (in black), or with isotype control Ab (white), and then analyzed by flow cytometry, as described in Methods. 20 Figure 2. Susceptibility to J-CD40L+ and J-CD40L- cells to CTLA- 4-FasL and CD40-FasL is dependent on expression of the relevant counterreceptors. A. CD40L-expressing (J-CD40L+) and non-expressing (J-CD40L-) malignant T cells (upper two panels), and Raji and Daudi malignant B cells (lower two panels), were plated (0.5 x 105) in round-bottom, 96-well 25 plates in the presence or absence of CTLA-4-FasL, CD40-FasL, or sFas (30 ng/ml each), or CTLA-4-Ig or CD40-Fc (100 ng/ml each, or indicated combinations. Cells were pulsed with [3H]thymidine and incubated for 18h. Assays were performed in triplicate. Data is presented as percentage of [3H]thymidine incorporation of cells in incubated in growth medium. The 9 225488/3 results shown summarize 3 independent experiments for each cell line. *P < 0.05 versus control, **P < 0.01 versus control. B. 0.5 x 105 Raji cells were incubated with (black bars) or without (white bars) CTLA-4-FasL (30 ng/ml) in the presence of either anti-CD80 or anti-CD86 Ab (B7 blockers) or their 5 combination for 18h. Proliferation was determined and presented as in A.

These results summarize three independent experiments. p< 0.05, * * p< 0.01 vs. medium.

Figure 3. Susceptibility of J-CD40L+ and J-CD40L- cells to apoptosis induction by CTLA-4-FasL and CD40· FasL is dependent on their io expression of relevant counter-receptors. J-CD40L+, CD40L (J-CD40L-), Raji and Daudi cells were incubated in the presence or absence of either CTLA-4-FasL, CD40-FasL, or sFas (30 ng/ml each), or CTLA-4Tg or CD40-Fc (100 ng/ml each, or indicated combinations, for 4h (T cells) or 16h (B cells). 26 Cells were harvested, co-stained with Annexin V and PI, and analyzed by 15 flow cytometry. A. A representative dot plot analysis of the cell lines in the presence or in the absence of CTLA-4-FasL or CD40-FasL. B-C. Percentage of dead cells (Annexin V+/PI- + Annexin V+/PI+) obtained from FACS analysis of Jurkat malignant T cell lines (B), Raji and Daudi malignant B cell lines (C). The summary of three independent experiments is shown. Data are presented as 20 mean + SD. ** p< 0.01 vs. medium.

Figure 4. CTLA-4-FasL and CD40-FasL each affects both apoptotic and anti-apoptotic signaling pathway elements. J-CD40L+ J-CD40L-, Raji and Daudi cells were incubated in the presence or absence of either CTLA-4· FasL, CD40-FasL, sFas, CTLA-4-Ig, CD40-Fc or their combinations as 25 indicated, for 90 min. Cells were collected, whole cell lysates were fractionated on 10% SDS-PAGE, and immunoblotted with the indicated Ab. A. Representative immunoblots for Jurkat malignant T cell lines and Raji and Daudi malignant B cell lines. Data shown is a representative experiment of at least three independent experiments for each cell line. B-C. Summary of three 30 independent experiments with the indicated Ab for Raji (B) and Jurkat (C) cell 10 225488/3 lines. The active forms of caspases 3, 9 and 8 are shown. Data were normalized against GAPDH. Expression of proteins in cells incubated in medium (control) were considered as 100%. Data are presented as mean + SD. * p < 0.05 vs. medium, ** p< 0.01 vs. medium. 5 Figure 5. CTLA4-FasL increases apoptotic signals and decrease the anti-apoptotic protein cFLIP in JY malignant B cells. JY cells were incubated in the presence or absence of either CTLA-4-FasL, CD40-FasL or anti-Fas Ab (CH11) for 90 min. Cells were collected, whole cell lysates were fractionated on 10% SDS-PAGE, and immunoblotted with the indicated Ab. A. 10 Representative immunoblots of three independent experiments for each cell line. B Summary of three independent experiments of JY malignant B cell lines with cFFIPs and anti-caspase 8 Ab C Summary of three independent experiments of JY malignant B cell lines with anti-caspase 9 and 3 Ab. Expression of proteins in cells incubated in medium (control) were considered 15 as 100%. Data are presented as mean + SD. * p < 0.05 vs. medium, ** p<0.01 vs. medium.

Figure 6. CTLA-4-Ig reduces proliferation of B7-expressing cells and affects their expression of caspases and cFLIP. A. Raji malignant B cells were incubated in the presence or absence of CTFA-4-FasF on flat-bottom 96 20 well plates that were pre-incubated with CTFA-4-Ig overnight. Assays were performed in triplicate. Cells were pulsed with [3H]thymidine, incubated for 24h and then evaluated for [3H]thymidine incorporation. [3H]thymidine incorporation of cells in medium (control) was designated as 100%, and the rest are presented as % of control, mean + SD. *P < 0.05 versus control, **P < 0.01 25 versus control. Data shown are a summary of three independent experiments. B-C. Raji cells were incubated on 24-well plates pre-coated with CTFA-4-Ig for 90 min. Cells were collected, whole cell lysates were fractionated on 10% SDS-PAGE, and immunoblotted with the indicated Ab. B. Representative immunoblots with the indicated Ab. C. Summary of three independent 30 experiments. The active forms of caspases 3, 9 and 8 are shown. Data were 11 225488/3 normalized against GAPDH. Expression of proteins in cells incubated in medium (control) were considered as 100%. Data are presented as mean + SD. * p < 0.05 vs. medium.

Figures 7 and 8 show that CTLA4-FasL induces death of both Raji and JY, B-5 cell lymphatic cancer cell lines, in a dose-dependent fashion.

Figure 9 shows the effect of CTLA4-FasL on RPMI 8226.

Figure 10 shows the effect of CTFA4-FasF on promyelocytic leukemia cells.

Figures 11-14 relate to the surface expression of CD80 and CD86, which bind CTAF4, and the expression of CD95 (Fas receptor, which binds FasF), in 10 various cell lines.

Figure 15 shows that CTFA4-FasF exhibited a cytotoxic effect against SK-Hepl hepatoma cells.

Figure 16 shows that the pan-caspase inhibitor, zVAD, completely abolished CTFA4-FasF effect. 15

DESCRIPTION OF EXEMPLARY EMBODIMENTS

According to at least some embodiments of the present invention, there is provided methods of treatment of lymphoma and/or multiple myeloma through the administration of chimeric proteins which are useful for both blocking and 20 signaling.

According to at least some embodiments, the chimeric protein is provided in a pharmaceutical composition, comprising the protein and a pharmaceutically suitable carrier. A pharmaceutical composition according to at least some embodiments of 25 the present invention can be administered via one or more routes of administration using one or more of a variety of methods known in the art. As 12 225488/3 will be appreciated by the skilled artisan, the route and/or mode of administration will vary depending upon the desired results. Preferred routes of administration for therapeutic agents of the invention include intravascular delivery (e.g. injection or infusion), intravenous, intramuscular, intradermal, 5 intraperitoneal, subcutaneous, spinal, oral, enteral, rectal, pulmonary (e.g. inhalation), nasal, topical (including transdermal, buccal and sublingual), intravesical, intravitreal, intraperitoneal, vaginal, brain delivery (e.g. intra-cerebroventricular, intra-cerebral, and convection enhanced diffusion), CNS delivery (e.g. intrathecal, perispinal, and intra-spinal) or parenteral (including 10 subcutaneous, intramuscular, intravenous and intradermal), transmucosal (e.g., sublingual administration), administration or administration via an implant, or other parenteral routes of administration, for example by injection or infusion, or other delivery routes and/or forms of administration known in the art. The phrase "parenteral administration" as used herein means modes of 15 administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural and intrasternal injection and 20 infusion.

As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible. Preferably, the carrier is suitable for intravenous, intramuscular, 25 subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the chimeric protein may be coated in a material to protect the protein from the action of acids and other natural conditions that may inactivate the protein. A pharmaceutical composition according to at least some embodiments of 30 the invention also may include a pharmaceutically acceptable anti-oxidant. 13 225488/3

Examples of pharmaceutically acceptable antioxidants include: (1) water soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfite, sodium sulfite and the like; (2) oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), 5 butylated hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol, and the like; and (3) metal chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like. Examples of suitable aqueous and nonaqueous carriers that may be employed in the pharmaceutical compositions according to at least some embodiments of 10 the invention include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in 15 the case of dispersions, and by the use of surfactants.

These compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents. Prevention of presence of microorganisms may be ensured both by sterilization procedures, supra, and by the inclusion of various antibacterial and antifungal agents, for 20 example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents which delay absorption such as aluminum monostearate and gelatin. 25 Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. The use of such media and agents for pharmaceutically active substances is known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use 30 thereof in the pharmaceutical compositions according to at least some 14 225488/3 embodiments of the invention is contemplated. Supplementary active compounds can also be incorporated into the compositions.

Therapeutic compositions typically must be sterile and stable under the conditions of manufacture and storage. The composition can be formulated as a 5 solution, microemulsion, liposome, or other ordered structure suitable to high drug concentration. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the 10 use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, or sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in 15 the composition an agent that delays absorption, for example, monostearate salts and gelatin. Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by sterilization microfiltration. Generally, dispersions are prepared by 20 incorporating the active compound into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freezedrying (lyophilization) that yield a powder of the active ingredient plus any 25 additional desired ingredient from a previously sterile-filtered solution thereof.

Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by sterilization microfiltration. Generally, dispersions are prepared by 30 incorporating the active compound into a sterile vehicle that contains a basic 15 225488/3 dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freezedrying (lyophilization) that yield a powder of the active ingredient plus any 5 additional desired ingredient from a previously sterile-filtered solution thereof.

The amount of active ingredient which can be combined with a carrier material to produce a single dosage form will vary depending upon the subject being treated, and the particular mode of administration. The amount of active ingredient which can be combined with a carrier material to produce a single 10 dosage form will generally be that amount of the composition which produces a therapeutic effect. Generally, out of one hundred per cent, this amount will range from about 0.01 per cent to about ninety-nine percent of active ingredient, optionally from about 0.1 per cent to about 70 per cent, optionally from about 1 per cent to about 30 per cent of active ingredient in combination 15 with a pharmaceutically acceptable carrier.

Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the 20 therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired 25 therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms according to at least some embodiments of the invention are dictated by and directly dependent on (a) the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding such an 30 active compound for the treatment of sensitivity in individuals. 16 225488/3

For administration of the chimeric protein, the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 5 mg/kg, of the host body weight. For example dosages can be 0.3 mg/kg body weight, 1 mg/kg body weight, 3 mg/kg body weight, 5 mg/kg body weight or 10 mg/kg body weight 5 or within the range of 1-10 mg/kg. An exemplary treatment regime entails administration once per week, once every two weeks, once every three weeks, once every four weeks, once a month, once every 3 months or once every three to 6 months.

Alternatively, the chimeric protein can be administered as a sustained 10 release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the chimeric protein in the patient. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic. In prophylactic applications, a relatively low dosage is administered at relatively infrequent 15 intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives. In therapeutic applications, a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete amelioration of symptoms of disease. Thereafter, the patient 20 can be administered a prophylactic regime.

Actual dosage levels of the active ingredients in the pharmaceutical compositions according to at least some embodiments of the present invention may be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, 25 composition, and mode of administration, without being toxic to the patient.

The selected dosage level will depend upon a variety of pharmacokinetic factors including the activity of the particular compositions according to at least some embodiments of the present invention employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate 30 of excretion of the particular compound being employed, the duration of the 17 225488/3 treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts. 5 A "therapeutically effective dosage" of a chimeric protein according to at least some embodiments of the invention preferably results in a decrease in severity of disease symptoms, an increase in frequency and duration of disease symptom-free periods, an increase in lifespan, disease remission, or a prevention of impairment or disability due to the disease affliction. For 10 example, for the treatment of Multiple Myeloma, lymphoma and/or leukemia, a "therapeutically effective dosage" optionally inhibits cell growth or tumor growth by at least about 20%, 40%, 60%, 80% relative to untreated subjects. The ability of a compound to inhibit tumor growth can be evaluated in an animal model system predictive of efficacy in human tumors. Alternatively, 15 this property of a composition can be evaluated by examining the ability of the compound to inhibit, such inhibition in vitro by assays known to the skilled practitioner. Alternatively or additionally, the suitable amount or dosage may also optionally be at least partially selected according to the administration of one or more additional treatments for multiple myeloma, which may optionally 20 and preferably have a synergistic effect and so which may optionally cause the dosage amount to be adjusted. Such one or more additional treatments could easily be selected by one of ordinary skill in the art.

Alternatively or additionally, a "therapeutically effective dosage" preferably results in at least stable disease, preferably partial response, more 25 preferably complete response, as assessed by the WHO or RECIST criteria for tumor response (Natl Cancer Inst 1999;91:523-8 and Cancer 1981;47:207-14). A therapeutically effective amount of a therapeutic compound can decrease tumor size, or otherwise ameliorate symptoms in a subject, or otherwise support partial or complete stable disease and/or partial or complete 30 response as determined above. One of ordinary skill in the art would be able to 18 225488/3 determine such amounts based on such factors as the subject's size, the severity of the subject's symptoms, and the particular composition or route of administration selected.

Therapeutic compositions can be administered with medical devices known in the art. For example, in a preferred embodiment, a therapeutic composition according to at least some embodiments of the invention can be administered with a needles hypodermic injection device, such as the devices disclosed in U.S. Pat. Nos. 5,399,163; 5,383,851; 5,312,335; 5,064,413; 4,941,880; 4,790,824; or 4,596,556. Examples of well-known implants and modules useful in the present invention include: U.S. Pat. No. 4,487,603, which discloses an implantable micro-infusion pump for dispensing medication at a controlled rate; U.S. Pat. No. 4,486,194, which discloses a therapeutic device for administering medicaments through the skin; U.S. Pat. No. 4,447,233, which discloses a medication infusion pump for delivering medication at a precise infusion rate; U.S. Pat. No. 4,447,224, which discloses a variable flow implantable infusion apparatus for continuous drug delivery; U.S. Pat. No. 4,439,196, which discloses an osmotic drug delivery system having multichamber compartments; and U.S. Pat. No. 4,475,196, which discloses an osmotic drug delivery system.

Many other such implants, delivery systems, and modules are known to those skilled in the art.

In certain embodiments, the chimeric protein according to at least some embodiments of the invention can be formulated to ensure proper distribution in vivo. For example, the blood-brain barrier (BBB) excludes many highly hydrophilic compounds. To ensure that the therapeutic compounds according to at least some embodiments of the invention cross the BBB (if desired), they can be formulated, for example, in liposomes. For methods of manufacturing liposomes, see, e.g., U.S. Pat. Nos. 4,522,811; 5,374,548; and 5,399,331. The liposomes may comprise one or more moieties which are selectively transported into specific cells or organs, thus enhance targeted drug delivery 19 225488/3 (see, e.g., V. V. Ranade (1989) J. Clin. Pharmacol. 29:685). Exemplary targeting moieties include folate or biotin (see, e.g., U.S. Pat. No. 5,416,016 to Low et al.); mannosides (Umezawa et al., (1988) Biochem. Biophys. Res.

Commun. 153:1038); antibodies (P. G. Bloeman et al. (1995) FEBS Lett. 5 357:140; M. Owais et al. (1995) Antimicrob. Agents Chemother. 39:180); surfactant protein A receptor (Briscoe et al. (1995) Am. J Physiol. 1233:134); p120 (Schreier et al. (1994) J. Biol. Chem. 269:9090); see also K. Keinanen; M. L. Laukkanen (1994) FEBS Lett. 346:123; J. J. Killion; I. J. Fidler (1994)

Immunomethods 4:273. 10 The following examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way. 15 EXAMPLE 1 - EFFICACY OF CHIMERIC PROTEIN FOR TREATMENT OF HEMATOLOGICAL MALIGNANCIES Materials and Methods

Cells, Abs, Reagents, and Fusion Proteins

Daudi, Raji, and JY and 293 human kidney cell lines were originally 20 obtained from the American Type Culture Collection (ATCC) (Bethesda, MD, USA). Two Jurkat sublines (J-CD40L+ and J-CD40L-) were kindly provided by Dr. John Fayen (CWRU, Cleveland, OH, USA). All cell lines were followed weekly to verify that they retained their original appearance and growing rates, and remained free of contamination. Also, cells were repeatedly tested to verify 25 continued expression of expected surface molecules. If any change was suspected, new lots were thawed. Medium was tested for mycoplasma contamination using a commercial PCR kit (Biological Industries, Israel). 20 225488/3 FITC-conjugated fluorescent Ab specific for CD40L, CD80, CD86 and CD95, along with their matched FITC-conjugated IgG isotypes, were purchased from PharMingen (San Diego, CA, USA). Recombinant human CTLA-4· Ig (CTLA-4/Fc) and sFasL were purchased from R&D Systems (MN, 5 USA) and Alexis Biochemicals (San Diego, CA), respectively. CD40-Fc fusion protein was purchased from Calbiochem ( Darmstadt, Germany). Anti-human B7-1 & B7-2 (CD80 and 86) Ab were purchased from R&D Systems. For Western blot analysis, anti-β actin Ab and anti-mouse GAPDH Ab were purchased from Sigma-Aldrich and Chemicon International, respectively. Anti- 10 FLIPS/L and anti-Caspase 8 pAb were purchased from Santa Cruz

Biotechnology (Santa Cruz, CA. USA) and MBL (Medical & Biological Laboratories Co, USA), respectively. Anti-Caspase 3 & 9 pAb were purchased from Cell Signaling Technology (Danvers, MA).

Culture medium for all experiments was RPMI 1640 (Biological 15 Industries, Israel), supplemented with 10% FBS (Invitrogen Life Technologies, CA), 2 mM L-glutamine, and 100 U/ml penicillin/streptomycin (Biological Industries). A hexahistidine-tagged derivative of CTLA-4· FasL (his6CTLA-4-FasL), with the tag appended to the amino terminus, and CD40-FasL were prepared as 20 previously described in Huang JH and Tykocinski ML (CTLA-4-Fas ligand functions as a trans-signal converter protein in bridging antigen-presenting cells and T cells. Int Immunol 2001, 13:529-539); Elhalel MD et al (CTLA-4. FasL induces alloantigen-specific hyporesponsiveness. J Immunol 2003, 170:58425850); and Dranitzki-Elhalel M et al (CD40.FasL inhibits human T cells: 25 evidence for an auto-inhibitory loop-back mechanism. Int Immunol 2007, 19:355-363), respectively.

Proliferation assays

Jurkat, Daudi or Raji cells in exponential growth phase were washed twice and re-suspended in medium at 1x106 cells/ml. 50 μΐ of cell suspension 21 225488/3 was added to individual wells of round-bottom 96-well tissue culture plates. CTLA- 4-FasL, CD40-FasL, sFasL, CTLA-4-Ig or CD40-Fc, or combinations of the latter three were added at different concentrations. Total culture volume was 200 μΐ/well. In some experiments, anti-CD80 or anti-CD86 Ab were added 5 20 min prior to the addition of CTLA-4-FasL. In other experiments, CTLA4-Ig in PBS was added to flat-bottom 96-well plates and incubated for 1 h at 370C and then overnight at 40C, in order to pre-coat the plates with CTLA4-Ig. The next day, plates were washed 4 times with PBS, and 5 x 104 Raji cells were added to each well and incubated for 24 h. Cultures were then pulsed with 0.5 10 pCi of [3H]thymidine (PerkinElmer, Waltham, Massachusetts, USA) and incubated at 37oC, 6% CO2 and 95% humidity for 18-24 hours. Cells were subsequently harvested onto glass fiber filters for scintillation counting. All proliferation assays were performed in triplicate. 15 Flow Cytometry

Cells were washed twice with FACS buffer (0.5% BSA/0.02% sodium azide in lx PBS) and incubated on ice for 30-45 min with one of the following; FITCconjugated anti-CD40L Ab, anti-CD80 Ab, anti-CD86 Ab, anti-CD95 Ab, 20 or their matching isotype controls, all purchased from PharMingen (San Diego, CA, USA). Flow cytometry was performed using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA, USA), and data were analyzed using CellQuest software (Becton Dickinson). A total of 1 x 105 events were collected for each sample. 25 To track cells undergoing apoptosis, 1 x 106 Jurkat T cells or 1.5 x 106

Raji or Daudi B cells were incubated in 24-well plates in a total volume of 1 ml, in the presence or absence of one of the following: CTLA-4-FasL, CD40-FasL, sFasL, CTLA4-Ig or CD40-Fc or combinations of the latter three. After 4 h (T cells) or 16 h (B cells), cells were collected and washed twice with 22 225488/3 cold FACS buffer (0.5% BSA/0.02% sodium azide in lx PBS). For detection of apoptosis and necrosis, cells were co-stained with propidium iodide (PI) and annexin-VFITC using a kit (MBL, Medical & Biological Laboratories Co, USA), according to the manufacturer’s protocol. Flow cytometry was 5 performed using a FACSCalibur flow cytometer, and data were analyzed using CellQuest software. A total of 1 x 105 events were collected for each sample. Whole cell lysates and Western blotting analysis

Jurkat, Raji, JY or Daudi cells in exponential growth phase were washed twice, resuspended in medium at 5 x 106 cells/ml, and plated in 24-well plates 10 in a total volume of 1 ml. CTLA-4-FasL, CD40-FasL, sFasL, CTLA4-Ig or CD40-Fc, or combinations of the latter three were added at different concentrations. After 90', cells were collected, washed twice with ice-cold PBS, and lysed in lysis buffer (0.5% Nonidet P-40, 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM PMSF, 1 mM sodium orthovanadate, 10 pg/ml leupeptin, and 15 10 pg/ml aprotinin) for 20-30 min on ice. The protein concentration of whole cell lysates was determined using the Bio-Rad Protein Assay Kit (Bio- Rad, Richmond, CA), according to the manufacturer’s protocol. Whole cell lysates were mixed with Laemmli sample buffer (Bio-Rad) at 1:1 ratio, heated for 10 min at 95°C, and equal amounts of protein were loaded onto 10% SDSPAGE. 20 Following electrophoresis, gels were blotted onto nitrocellulose membranes (Schleicher & Schuell), blocked with 5% milk/PBS, and probed overnight with primary Ab. After extensive washing, blots were incubated with HRP-conjugated matching secondary Ab (Bio-Rad), and developed with enhanced chemiluminescent substrate (Sigma-Aldrich) before exposure to Xray 25 film. Films were scanned and quantified by ImageMaster VDS-CL (Amersham Pharmacia Biotech). All membranes were re-blotted with either anti-β actin mAb or anti-GAPDH mAb to verify that similar quantities of protein were loaded on the gel.

30 RESULTS 23 225488/3

Fusion protein-mediated inhibition of cell proliferation is dependent on the surface expression of cognate receptors

As a first step, the functionality of the component parts of the CTLA-4-FasL

and CD40-FasL fusion proteins was established. To this end, malignant B 5 (Raji, JY and Daudi) and T (Jurkat) cell lines that differ in the expression of counter-receptors for these fusion protein elements - namely B7 (CD80 and CD86), CD40 ligand (CD40L) and Fas receptor (CD95) - were used. In the case of Jurkat, two sublines that differ in their expression of CD40L (J-CD40L+ versus J-CD40L-) 26 were paired. To start, surface expression of the 10 molecules on these various cell lines was verified by immunofluorescence and flow cytometry (Figure 1). As expected, the B and T cell lines were positive and negative for B7 molecules, respectively, and Daudi cells expressed negligible levels of Fas receptor. The difference in CD40L expression between the J-CD40L+ and J-CD40L- T cell sublines was also confirmed. 15 Next, it was determined whether the differences in cognate surface receptor expression correlate with the abilities of CTLA-4-FasL and CD40-FasL to inhibit the proliferation of the tumor lines (Figure 2A). Specifically, the different cell lines were pulsed with [3H]thymidine for 16-20 h in the presence or absence of CTLA-4-FasL, CD40-FasL, CTLA4-Ig, CD40-Fc, sFasL, or 20 different combinations of the latter three. As expected, the proliferation of

Daudi cells, which express minimal Fas receptor, was not inhibited by either of the FasL-containing fusion proteins, CTLA-4-FasL and CD40-FasL, nor by sFasL (Figure 2A, lowest panel), highlighting the Fas-dependence of their inhibitory activity. In contrast, these fusion proteins significantly inhibited 25 proliferation of the other two malignant B cell lines, Raji (Figure 2A) and JY (not shown), which do express the Fas death receptor. CTLA-4-FasL was substantially more potent than sFasL and CTLA4-Ig, alone or in combination, 24 225488/3 in inhibiting proliferation of the B7-expressing malignant B cells. Indeed, CTLA4Ig had no effect on proliferation at concentrations as high as lOOng/ml.

Of note, CTLA-4 FasL was significantly more effective than CD40 FasL against the B cell lines (which are negative for CD40L). In fact, CD40 FasL, at 5 concentrations up to 30 ng/ml, had no inhibitory effect on the B cell lines.

To further explore the requirement for CD40L expression on target cells for CD40*FasL's activity, advantage was taken of the Jurkat T cell derivative lines that differ in CD40L expression (J-CD40L+ versus J-CD40L-). Both of these T cell lines were inhibited by sFasL and FasL-containing fusion proteins 10 to a greater extent than Raji B cells (Figure 2A, upper two panels), with the JCD40L- line being the most sensitive. Interestingly, as shown previously, only cells that express CD40L (J-CD40L+) were affected by low concentrations of CD40*FasL, though at higher concentrations of CD40*FasL (e.g. >100 ng/ml), all Fas-expressing cells were inhibited to some degree (not shown). 15 Given that T cells were highly sensitive to all forms of sFasL, it was not possible to use them as B7-negative controls for establishing the requirement for B7 surface expression in CTLA-4*FasL's activity. Consequently, an Ab blocking experiment was performed using B7-positive B cells as targets. In particular, CTLA-4·FasL’s inhibitory effect in the absence or presence of 20 antagonistic Ab against CD80 and CD86 (Figure 2B) was compared. Anti-CD80 blocking Ab (0.5 or 1 pg/ml) significantly attenuated the inhibitory effect of CTLA-4-FasL (30ng/ml). In contrast, anti-CD86 Ab, at the same concentrations, had no effect on CTLA-4-FasL inhibition of Raji cell proliferation. The addition of the two blocking Ab in combination completely 25 abolished CTLA-4-FasL's inhibitory effect. Taken together, these data indicate that CTLA-4-FasL and CD40-FasL exhibit maximal inhibitory potency when the target malignant lymphoid lines express cognate counter-receptors for both domains of each fusion protein. 25 225488/3

Fusion protein-mediated induction of apoptosis is dependent on the surface expression of cognate receptors

Previous studies have established that both CTLA-4*FasL and CD40*FasL induce their inhibitory effect via Fas-mediated apoptosis. It was 5 then considered whether apoptosis induction by these fusion proteins parallels proliferative inhibition in being more efficient when target cells co-express surface molecules that can bind both ends of the respective proteins. To this end, the same set of malignant cell lines were incubated for 4 (T cells) or 16 (B cells) hours in the presence or absence of CTLA-4-FasL , CD40-FasL , 10 CTLA4-Ig, CD40-Fc, sFasL, or different combinations of latter three. At the end of the treatment period, cellular apoptosis and necrosis were assessed using Annexine/PI staining and flow cytometry. Again as expected, FasLcontaining fusion proteins or sFasL had no effect on Daudi B cells lacking Fas receptor, and neither CTLA-4-Ig or CD40-Fc induced apoptosis in the Daudi cells 15 (Figure 3A, B). By contrast, and as was the case in the proliferation assay, CTLA-4-FasL significantly increased the percentage of apoptotic or necrotic Raji cells at 16 h (Figure 3A, B). Again, more apoptosis was detected when Raji cells were incubated with CTLA-4-FasL , as opposed to sFasL, CTLA-4-Ig, or a combination of the latter two. No significant apoptosis of Raji cells was 20 induced by CD40-FasL (Figure 3A, C).

When Jurkat T cells (either J-CD40L+ or J-CD40L-) were incubated with CTLA-4-FasL , significant apoptosis ensued, though J-CD40L+ were less affected than J-CD40L- (Figure 3A, C). The same difference in susceptibility to apoptosis induction between these paired cells lines was evident when sFasL or 25 the anti-Fas Ab CHI 1 (not shown) was used. However, when the Jurkat cell lines were incubated in the presence of CD40-FasL , significant apoptosis could be detected only in J-CD40L+ cells, despite the fact that sFasL was more potent in inducing apoptosis in J-CD40L- cells (Figure 3A, C). This set of experiments highlights the functional importance of both ends of each of the 30 respective fusion proteins for efficient apoptosis induction. 26 225488/3

Both pro-apoptotic and anti-apoptotic pathways are affected by CTLA-

4-FasL and CD40-FasL

The possibility that the apoptosis-inducing activity of CTLA-4 FasL and 5 CD40 Fas may not be solely reliant on triggering of the Fas death receptor was then considered. To test this hypothesis, the T and B cell lines were incubated for 90-180 min with CTLA-4· FasL , CD40-FasL , sFasL, CTLA-4· Ig, CD40 or combinations of the latter three. At the end of the incubation period, whole cell lysates were evaluated by immunoblotting for expression of the anti-apoptotic 10 protein cFLIP, caspase 8 (as a marker of the extrinsic pathway), caspase 9 (as a marker of the intrinsic, mitochondrial pathway), and caspase 3. Of note, both the pro-caspase forms (not shown) and the cleaved, active caspase forms were examined. A representative experiment is shown in Figure 4A, and the summary of 3 independent experiments performed with the different cell lines 15 is shown in Figures 4 B-D.

The expression of the different caspases in the Daudi B cell line (with negligible Fas receptor) did not change after incubation with the FasLcontaining proteins (Figure 4A). Furthermore, no change was found when Daudi cells were incubated with CTLA-4· Ig, CD40-Fc or a combination of the 20 latter with sFasL (Figure 4A). In contrast, B cell lines co-expressing B7 molecules and Fas receptors yielded an entirely different picture. After 90 or 180 minutes of incubation in the presence of CTLA-4· FasL , cFLIPL expression was abrogated, and a clear increase in the activated (cleaved) forms of two of the caspases (9 and 3) was observed. Since caspase 8 is found mainly 25 in its active, cleaved form in these cells, even when Fas receptor is not triggered and cFLIP base levels are very low, another malignant B cells line, JY, also co-expressing B7 molecules and Fas receptor, was considered.

When incubated with CTLA-4· FasL, there was a clear decrease in cFLIP abundance and a significant increase in the activated forms of caspases 3, 9, 30 and 8 (Figures 5A-C). In contrast, CD40-FasL , sFasL, CTLA-4· Ig, or CD40- 27 225488/3

Fc had no effect on cFLIP expression or caspase activation in either of the malignant B cell lines (Figures 4 and 5).

The next focus was on CD40 FasL with an appropriate cell target, J-CD40L+. When the J-CD40L+ and J-CD40L- T cell lines were incubated with 5 CTLA-4-FasL or CD40-FasL, the pattern expression of apoptotic and anti-apoptotic protein expression was distinctly different from that seen with the B cell lines (Figure 4A, upper two panels; Figure 4C). In both Fas-expressing Jurkat cell lines, CTLA-4-FasL and CD40-FasL each activated the caspase cascade, showing increased abundance of the activated forms of caspase 9 and 10 3.

Caspase 8 appears in its activated form in these cell lines. No change in cFLIP abundance was noted. However, J-CD40L+ cells, though less sensitive to CTLA-4-FasL than J-CD40L- (as one reflected in the lesser increase in the activated forms of caspase 9 and 3), responded to CD40-FasL treatment with a 15 significant decrease in cFLIP abundance, and additional activation of caspases 8, 3 and 9. CD40-FasL was more potent in doing so than CD40-Fc, sFasL or a combination of the two. Taken together, these experiments establish that CTLA-4-FasL and CD40-FasL each has dual and reinforcing effects on both apoptotic (caspase) and anti-apoptotic (cFLIP) pathways.

20 CTLA-4Tg inhibits proliferation of a B cell line and decreases cFLIP levels. One possible explanation for the special properties of the fusion proteins, with unique impact on anti-apoptotic signaling, is back-signaling through the non-Fas surface counter-receptors for the fusion protein components. Indeed back-signaling through the ‘costimulatory ligands’ B7 (on 25 antigen-presenting cells) and CD40L (on T cells) has been documented by others. It was therefore considered whether the exceptional potency of the CTLA-4-FasL and CD40-FasL fusion proteins might be explained, at least in part, by backsignaling through B7 and CD40L, respectively, occurring simultaneously with Fas receptor triggering. For that purpose, Raji B cells were 30 treated with CTLA-4-FasL for 24h, in the presence or absence of plate-bound 28 225488/3 CTLA-4-Ig, and proliferation was assessed. As shown in Figure 6A, plate-bound CTLA-4-Ig alone significantly inhibited Raji cell proliferation. CTLA-4-FasL-mediated inhibition of Raji cell proliferation was diminished in the presence of plate bound CTLA-4-Ig, suggesting interference with CTLA- 5 4-FasL binding to B7 (Figure 6B). Significantly, plate-bound CTLA-4-Ig, like CTLA-4-FasL, reduced cFLIP expression in Raji cells and increased the expression of caspases 9 and 3 in them (Figure 6C). These findings suggest that CTLA-4-Ig can deliver an inhibitory back-signal to malignant B cells and reduce anti-apoptotic cFLIP in them. 10 It was considered whether CD40-FasL can mediate similar back-signaling through CD40L. In order to test this possibility, J-CD40L+ cells were incubated with CD40-Fc. CD40-Fc increased J-CD40L+ proliferation by -20% (not shown). However, no effect on the expression of the apoptotic and antiapoptotic proteins was observed when CD40-Fc was used at concentrations 15 up to 1200 ng/ml (data not shown).

DISCUSSION

In this study, the unique functional properties of two fusion proteins, CTLA-4-FasL and CD40-FasL, as inducers of apoptosis in malignant 20 lymphoid cell lines have been considered, focusing on relevant intracellular signaling cascades. Without wishing to be limited by a single hypothesis or a closed list, these findings include: 1) CTLA-4-FasL and CD40-FasL induce death in Fas receptor-expressing malignant lymphoid lines of both B and T lineages; 2) CTLA-4-FasL induces apoptosis in B7-expressing B cell lines 25 more potently than does CD40-FasL; 3) CD40-FasL induces apoptosis in CD40L-expressing J-CD40L+ T cells in a CD40L-dependent fashion; 4) CTLA-4-FasL lowers cFLIP expression and activates the caspase cascade in cells co-expressing both B7 and Fas receptor at their surfaces; 5) CD40-FasL lowers cFLIP expression and activates the caspase cascade in cells co 29 225488/3 expressing both CD40L and Fas receptor; and 6) CTLA-4-FasL and CD40-FasL are each more effective in inducing apoptosis than either of their component parts, alone or in combination.

Taken together these findings affirm the higher order functionality of 5 these unique fusion proteins, and suggest that they may have special advantages for inducing apoptosis in malignant lymphoid cells, by coordinately affecting apoptotic and anti-apoptotic pathways. One of the mechanisms enabling malignant cells to escape apoptosis, and thereby remain refractory to treatment, is up-regulation of anti-apoptotic proteins. FLIP, expressed either 10 intrinsically (as cFLIP) or extrinsically (as vFLIP), has been implicated as a key anti-apoptotic protein in several malignant lymphomas. It has been previously shown that CTLA-4-FasL prevents the up-regulation of cFLIP expression that normally accompanies T cell activation. By abrogating upregulation of this anti-apoptotic protein, CTLA-4-FasL is able to induce 15 apoptosis in activated T cells at an earlier phase than does sFasL. The present study extends this functional feature of CTLA-4-FasL to transformed cells.

For most of the cell lines studied here (that is, J-CD40L+ , J-CD40L- and JY cells), reduction in cFLIP expression was correlated with activation of caspases 3, 8, and 9. Caspase 8 activation is dependent on its binding to the 20 death complex FADD. This binding leads to activation of caspase 3, and Bid, that in turn results in cytochrome c and caspase 9 activation. Activation of caspase 9 then leads to more caspase 3 activation and effective apoptosis. The ability of cFLIP proteins to inhibit caspase 8 activation, by competing with caspase 8 for binding to the death complex FADD, is well-established. 25 There are contradictory data in the literature concerning the role of the two splice variants of cFLIP: cFLIP short (cFLIPs) and cFLIP long (cFLIPL). cFLIPL’s role in the system appears to be more complicated, with data indicating that high levels of expression lead to apoptosis, whereas moderate levels result in the opposite, that is, inhibition of Fas-mediated apoptosis in 30 vitro and in vivo. However, data are clearer in the case of cFLIPL under- 30 225488/3 expression, in that selective silencing of cFLIPL mRNA augments caspase 8 recruitment, activation, processing and release from the death complex and hence enhanced apoptosis. Only the cFLIPL splice variant was detected in the Raji B cell line, and CTLA-4-FasL, able to bind to both B7-1 (CD80) and Fas 5 receptor (CD95) on these cells, decreased expression of this cFLIP isoform. Significantly, despite the constitutive expression of the activated form of caspase 8 in these Raji cells, CTLA-4· FasL-driven cFLIPL reduction was correlated with activation of caspases 9 and 3 and effective induction of apoptosis. Without wishing to be limited by a single hypothesis, reduction of c- 10 FLIP by CTLA-4· FasL and CD40-FasL may lead to both pro-apoptotic and anti-proliferative effects in transformed lymphoid cells.

By chimerizing FasL to CTLA-4 and CD40, one creates intriguing molecular bridging possibilities. Transformed B cells introduce a new scenario wherein the same fusion protein can potentially bridge the counter-receptors 15 being co-expressed on the same cell. Intercellular and intracellular bridging by both CTLA-4· FasL and CD40-FasL are certainly not mutually exclusive, and one can envision both taking place in the context of local tumor growth.

The cis loop-back auto-signaling mechanism may lead to more effective inhibition on more than one basis. First, the fusion proteins serve to tether FasL 20 to membranes, via either CTLA-4:B7 binding on B cells or CD40:CD40L binding on T cells. The potency of surface-anchored FasL has been clearly established in the context of exogenously-introducing it onto APC surfaces to generate deletional APC 56-59. One would thus expect fusion protein-tethered FasL to be highly functional in an auto-signaling mode as well. Second, both 25 CTLA-4· FasL and CD40-FasL have the potential to act as dual-signaling agents by triggering neighboring counter-receptors on the same cell surface.

Back-signaling through the B7 molecules CD80 and CD86 has been described for B cells 23, leading to proliferative inhibition of B cells in the case of CD80 and the opposite for CD86. Since CTLA-4 has higher avidity for 30 CD80, one might expect the CD80-related effect to dominate for CTLA- 31 225488/3 4-FasL. Data here showing that CTLA-4-Ig inhibits proliferation of CD80-expressing Raji cells is consistent with this back-signaling function.

Back-signaling has also been demonstrated for CD40L on T cells, and thus could also contribute to CD40-FasL’s observed efficacy. In the case of 5 CD40-FasL, but not CD40-Fc, the CD40 moiety is being presented in a cellbound mode (via FasL:Fas anchoring), and with the likelihood of being in a trimer or two-trimer configuration (versus the presumed CD40-Fc dimer).

Indeed, it has been shown that CD40-Fc has lower affinity for CD40L than do oligomers of CD40, and this higher affinity is accompanied by stronger 10 biological activity. Taken together, but without wishing to be limited by a single hypothesis, these various data suggest that not only can each of CTLA-4· FasL and CD40-FasL deliver dual signals on the same cell, but in both instances, the Fas signaling can be potentiated by backsignaling through the other counter-receptor (B7-1 or CD40L). 15 Of note, CTLA-4-FasL was found to be a potent inducer of apoptosis in

Jurkat T cells, even though these cells are negative for B7 proteins. This likely reflects their overall higher susceptibility to soluble FasL-mediated apoptosis, as evidenced by their high sensitivity (especially the J-CD40L- subline) to sFasL and agonistic anti-Fas Ab (CH11; data not shown). 20 The present study highlights the functional richness of appropriately configured fusion proteins. The CTLA-4-FasL and CD40-FasL fusion proteins modulate both normal and transformed lymphoid cells, serve to bridge molecules intercellularly and intracellularly, set up artificial cis loop-back autosignaling loops, and deliver dual-signals that are reinforcing. Both of these 25 proteins can down-modulate anti-apoptotic cFLIP isoforms, an effect that serves to potentiate the FasL pro-apoptotic signal that these same proteins deliver. Thus, one can leverage fusion proteins to simultaneously deliver a death signal and sensitize the cells to it. 32 225488/3

EXAMPLE 2 - CLINICAL TRIAL OF A CHIMERIC PROTEIN FOR TREATMENT OF A HEMATOLOGICAL MALIGNANCY A phase I trial of a chimeric protein (therapeutic agent) as described herein to subjects with a hematological malignancy selected from the group 5 consisting of lymphoma, multiple myeloma and a leukemia as recited herein, may be designed to evaluate both effect on disease progression and possible toxicity. Subjects with a suspected hematological malignancy may be enrolled after positive diagnosis of the hematological malignancy as is well known in the art. 10 Treatment may be provided as a single therapeutic or in combination with accepted treatments. For example, treatment strategies for multiple myeloma are reviewed in Rajkumar et al., 2002, Mayo Clin. Proc. 77:814. Recognition of acute or unusual progression of the disease may halt administration of therapeutic agent. 15 Initial subjects receive a suitable dosage of therapeutic agent, administered for example as a 1 hour intravenous infusion or as appropriate, alone or in combination with one or more other known agents as could be selected by one of ordinary skill in the art. Given adequate tolerance, the dose will be increased stepwise in subsequent subjects. Additionally, the method for 20 20 administration may be changed to bolus injection.

Toxicity of therapeutic agent is evaluated in subjects according to the World Health Organization Toxicity Criteria: blood pressure, temperature and heart rate are monitored every 10 minutes during infusion, then every hour for 3 hours and finally every 3 hours for 24 hours. Hematologic, renal and liver 25 function tests are conducted every other day for one week and on day 15, 30, 60 and 120 post injection.

Serum and/or tissue samples are obtained once a week for two months so that the effects of the therapeutic agent may be determined by methods known in the art, e.g., change serum concentration of M protein, change in hemoglobin 33 225488/3 value, presence/regression of lytic bone lesions etc. Pathologic studies will assess treatment effect on tissue damage associated with the hematological malignancy. 5 Example 3- Effect on Tumor Cell Lines

Materials and methods.

Unless otherwise stated, all chemicals were obtained from SIGMA (Israel). DMEM medium, FBS, PBS, Trypsin-EDTA, penicillin, streptomycin and L-Glutamine were obtained from Biological Industries (Beit Haemek, 10 Israel).

Cell lines. Raji (EBV transformed B cell lymphoma line), JY (EBV transformed B cell lymphoma line), Daudi (EBV transformed B cell lymphoma line) RPMI 8226 (human multiple myeloma cell line), HL60 (Human promyelocytic leukemia cells) were purchased from ATCC (USA). SK-HEP-1 15 (HTB-52; liver adenocarcinoma cell line) was purchased from ATCC (USA).

HepG2, Huh7 hepatocellular carcinoma cell lines, originally from the ATCC, were kindly provided by the Hepatology Unit, Hadassah Hebrew University Medical Center in Jerusalem, Israel. Unless otherwise stated, all cell lines were grown in 10% FBS DMEM supplemented with lOU/ml penicillin, O.lmg/ml 20 streptomycin and 292 pg/ml L-Glutamine. All cell lines were cultured at 37°C in 6% CO2 and cultures were tested periodically for mycoplasma contamination using EZ-PCR mycoplasma test kit (Biological Industries).

Activity assay. To assess KAHR-102 (CTLA4-FasL) activity, 0.2 x 106 cells/ml were seeded as triplicates in 96-well plates (NUNC, Roskilde, 25 Denmark) and cultured with or without varying concentrations of the histidine tagged version of KAHR-102 (his6CTLA4-FasL), Cells were incubated with the indicated protein concentration for 24h at 37°C in 6% CO2, and cell viability was evaluated using MTS assay (Promega, Madison, USA). 34 225488/3

Flow Cytometry. To assess apoptosis, 0.2 x 106 cells/ml were seeded as duplicates in 24-well plates (NUNC) and incubated with or without varying concentrations of his6CTLA4-FasL (KAHR-102), soluble FasL, CTLA4-Fc or the latter in combination for 24 h. Cells were then harvested, and apoptotic 5 cells were detected by flow cytometric analysis, using the AnnexinV/PI MEBCYTO Apoptosis Kit (MBL, Nagoya Japan), according to the manufacturer’s protocol. 20,000 events per sample were counted using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA, USA), and data were analyzed using CellQuest software (Becton Dickinson). 10 To examine expression of Fas receptor and the B7 molecules, CD80 and CD86, in the different cell lines, cells were retrieved, washed in staining buffer (PBS containing 1% BSA and 0.1% sodium azide), and stained with phycoerythrin-labeled mAb with specificity for the above mentioned molecules or the relevant control Abs, at concentrations recommended by the 15 manufacturer (eBioscience). Flow cytometry was performed using a FACSCalibur flow cytometer (Becton Dickinson), and data were analyzed using CellQuest software (Becton Dickinson). A total of 20,000 events were collected for each sample.

Statistical analysis. Data are presented as means + SD. Statistical 20 comparison of means was performed by a two-tailed unpaired Student's t test. Differences with a p<0.05 were considered statistically significant.

Establishment of Tumor Xenografts. Athymic BALB/c nu/nu nude male mice (Harlan, Israel), 4-6 weeks of age, maintained under defined flora conditions at the Hebrew University Pathogen-Free Animal Facility will be 25 used. All experiments were approved by the Animal Care Committee of the Hebrew University. Raji, JY or RPMI 8226 grown to 80% confluence, harvested, washed with PBS, and injected subcutaneously (2 x 107/mouse) into the right flanks of mice or intraperitoneal. Once palpable, tumors will be measured for their widths and lengths using a micro-caliper for the sub- 30 cutaneous tumors, and tumor volumes were calculated (w2 x length /2), or, for 35 225488/3 the intraperitoneal tumors, abdominal diameter will be measured, and mice will be weighted. Mice will treated daily with subcutaneous injections of his6CTLA4-FasL (KAHR-102), (200 pg) for 8 days. If tumor will re-grow or re-appear, another treatment will follow in two weeks. Control groups will be 5 injected with similar volumes of the his6CTLA4-FasL (KAHR-102) dilution buffer. Tumor volumes will monitored for approximately a 3 month, or until tumor size exceeded the threshold requiring sacrifice of the animal. At the end of experiments, mice will be sacrificed, and tumors will be harvested, measured and weighed, and analyzed. io Results CTLA4-FasL's cytotoxic activity was evaluated against different lymphoma cells. As shown in Figures 7 and 8, CTLA4-FasL induces death of both Raji and JY, both of which are B-cell lymphatic cancer cell lines, in a dose-dependent fashion. Of note, significant cell death was detected at a 15 concentration as low as 0.1 ng/ml, corresponding to an EC50 of 0.4 nmol/1.

Cell death was detected at 24h, and was more robust at 48h, with almost no live cells detectable at 3 ng/ml CTLA4-FasL. Importantly, primary B cells taken from healthy volunteers were resistant to CTLA4-FasL's toxic activity (not shown). 20 The human myeloma cell line, RPMI 8226, was then tested and as seen in

Figure 9 found that though their sensitivity to CTLA4-FasL is lower than that of the B cell lymphoma cell line, they do respond to higher concentrations of CTLA4-FasL. In contrast, promyelocytic leukemia cells were resistant to CTLA4-FasL's cytotoxic effect (Figure 10). 25 The difference in susceptibility to CTLA4-FasL's action can be explained by the different expression of the surface molecules capable of binding the fusion protein. The expression of CD80 and CD86, which bind CTAL4, and the expression of CD95 (Fas receptor, which binds FasL) were then tested on the four cell lines. As can be seen in Figures 11-14, the two highly sensitive cell 36 225488/3 lines, namely Raji and JY, express all three surface molecules. The RPMI 8226, human multiple myeloma cell line, that exhibited intermediate sensitivity to CTLA4-FasL express both the Fas receptor and CD86, however in lower levels than the B cell lines, and do not express CD80 at all. The promyelocitic 5 leukemia cells, that are not affected by CTLA4-FasL show low level of expression of CD86, and very low levels of Fas receptor.

The analysis was extended to other cancer cell lines, namely, SK-Hepl hepatoma cells. As can be seen in Figure 15, CTLA4-FasL exhibited a cytotoxic effect against this tumor line, albeit with somewhat different kinetics; 10 CTLA4-FasL was by far more potent than CTAL4-Fc, soluble FasL or the combination of the latter. Using this cell line, the effect of inhibition of apoptosis mediated by caspases on CTLA4-FasL's effect was tested. As shown in Figure 16, the pan-caspase inhibitor, zVAD completely abolished CTLA4-FasL effect, indicating that its cytotoxic effect is apoptosis based.

Whereas particular embodiments of this invention have been described above for purposes of illustration, it will be evident to those skilled in the art that numerous variations of the details of the present invention may be made 20 without departing from the invention as defined in the appended claims.

It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the 25 invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination. 37 225488/3

Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall 5 within the spirit and broad scope of the appended claims. 38