4-amino-4-oxobutanoyl peptides as inhibitors of viral replication

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority from U.S. provisional patent application nos. 60/830,488, filed July 13, 2006 and 60/945,786, filed June 22, 2007, both of which are hereby incorporated by reference in their entirety.

FIELD OF THE INVENTION

[0002] The present invention provides 4-amino-4-oxobutanoyl peptides, useful as antiviral agents. Certain 4-amino-4-oxobutanoyl peptides disclosed herein are potent and/ or selective inhibitors of viral replication, particularly Hepatitis C virus replication. The invention also provides pharmaceutical compositions containing one or more 4-amino-4-oxobutanoyl peptides and one or more pharmaceutically acceptable carriers, excipients, or diluents. Such pharmaceutical compositions may contain 4-amino-4-oxobutanoyl peptide as the only active agent or may contain a combination of a 4-amino-4-oxobutanoyl peptide or related compound and one or more other pharmaceutically active agents. The invention also provides methods for treating viral infections, including Hepatitis C infections, in mammals.

BACKGROUND

[0002] An estimated 3% of the world’s population is infected with the hepatitis C virus. Of those exposed to HCV, 80% become chronically infected, at least 30 % develop cirrhosis of the liver and 1-4% develop hepatocellular carcinoma. Hepatitis C Virus (HCV) is one of the most prevalent causes of chronic liver disease in the United States, reportedly accounting for about 15 percent of acute viral hepatitis, 60 to 70 percent of chronic hepatitis, and up to 50 percent of cirrhosis, end-stage liver disease, and liver cancer. Chronic HCV infection is the most common cause of liver transplantation in the U.S., Australia, and most of Europe. Hepatitis C causes an estimated 10,000 to 12,000 deaths annually in the United States. While the acute phase of HCV infection is usually associated with mild symptoms, some evidence suggests that only about 15% to 20% of infected people will clear HCV.

[0003] HCV is an enveloped, single-stranded RNA virus that contains a positive-stranded genome of about 9.6 kb. HCV is classified as a member of the Hepacivirus genus of the family Flaviviridae. At least 4 strains of HCV, GT-1 - GT-4, have been characterized.

[0004] The HCV lifecycle includes entry into host cells; translation of the HCV genome, polyprotein processing, and replicase complex assembly; RNA replication, and virion assembly and release. Translation of the HCV RNA genome yields a more than 3000 amino acid long polyprotein that is processed by at least two cellular and two viral proteases. The HCV polyprotein is:

NH2-C-El-E2-p7-NS2-NS3-NS4A-NS4B-NS5A-NS5B-COOH.

[0005] The cellular signal peptidase and signal peptide peptidase have been reported to be responsible for cleavage of the N-tenninal third of the polyprotein (C-El-E2-p7) from the nonstructural proteins (NS2-NS3-NS4A-NS4B-NS5A-NS5B). The NS2-NS3 protease mediates a first cis cleavage at the NS2-NS3 site. The NS3-NS4A protease then mediates a second ciscleavage at the NS3-NS4 A junction. The NS3-NS4A complex then cleaves at three downstream sites to separate tire remaining nonstructural proteins. Accurate processing of the polyprotein is asserted to be essential for forming an active HCV replicase complex.

[0006] Once the polyprotein has been cleaved, the replicase complex comprising at least the NS3-NS5B nonstructural proteins assembles. The replicase complex is cytoplasmic and membrane-associated. Major enzymatic activities in the replicase complex include serine protease activity andNTPase helicase activity in NS3, and RNA-dependent RNA polymerase activity of NS5B. In the RNA replication process, a complementary negative strand copy of the genomic RNA is produced. The negative strand copy is used as a template to synthesize additional positive strand genomic RNAs that may participate in translation, replication, packaging, or any combination thereof to produce progeny virus. Assembly of a functional replicase complex has been described as a component of the HCV replication mechanism. Provisional application 60/669,872 “Pharmaceutical Compositions and Methods of Inhibiting HCV Replication” filed April 11,2005, is hereby incoiporated by reference in its entirety for its disclosure related to assembly of the replicase complex.

[0007] Current treatment of hepatitis C infection typically includes administration of an interferon, such as pegylated interferon (TEN), in combination with ribavirin. The success of current therapies as measured by sustained virologic response (SVR.) depends on the strain of HCV with which the patient is infected and the patient’s adherence to the treatment regimen. Only 50% of patients infected with HCV strain GT-1 exhibit a sustained virological response. Direct anting antiviral agents such as ACH-806, VX-950 and NM 283 (prodrug of NM 107) are in clinical development for treatment of chronic HCV. Due to lack of effective therapies for treatment for certain HCV strains and the high mutation rate of HCV, new therapies are needed. The present invention fulfills this need and provides additional advantages, which are described herein.

SUMMARY OF THE INVENTION

[0008] The invention provides compounds of Formula I (shown below) and includes 4amino-4-oxobutanoyl peptides and related compounds. Certain 4-amino-4-oxobutanoyl peptides disclosed herein possess antiviral activity. The invention provides compounds of Formula I that are potent and/ or selective inhibitors of Hepatitis C virus replication. The invention also provides pharmaceutical compositions containing one or more compound of Formula I, or a salt, solvate, or acylated prodrug of such compounds, and one or more pharmaceutically acceptable carriers, excipients, or diluents.

[0009] The invention further comprises methods of treating patients suffering from certain infectious diseases by providing to such patients an amount of a compound of Formula I effective to reduce signs or symptoms of the disease or disorder. These infectious diseases include viral infections, particularly HCV infections. ׳The invention particularly includes methods of treating human patients suffering from an infectious disease, but also encompasses methods »f .treating other animals, including livestock and domesticated companion animals, suffering from an infectious disease.

[0010] Methods of treatment include providing a compound of Formula I as a single active agent or providing a compound of Formula I in combination, with one or more other therapeutic agents.

[0011] Thus in a first aspect the invention includes compounds of Formula I pharmaceutically acceptable salts thereof:

<img file="IL196256A_D0001.tif" />

[0012] Within Formula I the variables, e.g. R!-R₉, R16, R!8, R!9, Μ, Y, Z, and n, cany the definitions set forth below.

[0013]------represents a double or single covalent bond, and the group

<img file="IL196256A_D0002.tif" />

'*Y'' contains 0 or 1 double bonds.

[0014] R! is -NRioRn, -(OOjNRjoRii, -(C=S)NR1qRh, -(C=O)Ri2, -SO2Rj2, ־ (C=O)OR12, -O(C=O)R!2,-OR12. or-N(C=O)R12, andR2 is hydrogen, C!-C6alkyl, C₃C7cycloalkyl, heterocycloalkyl, (aryl)C0־C4alkyl.

[0015] Or, R! and R2 are taken together to form an optionally substituted 5- to 7membered heterocyclic ring containing 0 or 1 additional N, S, or O atoms, or an optionally substituted 5- to 7-membered heterocyclic ring containing 0 or 1 additional N, S, or O atoms fused to an optionally substituted 5- to 7-membered carbocyclic or heterocyclic ring.

[0016] R3, R4, R5, R6. R7, and R8 are independently (a) hydrogen, halogen, or amino, or (b) C1-C₆alkyl, C₂-C₆alkenyl, (C₃־C₇cycloalkyl)C(rC4aIkyl, (C₃-C₇cycloalkenyl)C₀C4alkyl, (hcterocycloalkyl)C0-C4alkyl, C₂-C6alkanoyl, or mono- or di- C1-C6alkylamino, each of which is optionally substituted.

[0017] R3 and R4 may be joined to form an optionally substituted 3- to 7־ membered cycloalkyl ring or an optionally substituted 3— to 7-membered heterocycloalkyl ring containing 1 or 2 heteroatoms independently chosen from N, S, and O.

[0018] Rs and K5 may be joined to form an optionally substituted 3- to 7- membered cycloalkyl ring or an optionally substituted 3— to 7-membered heterocycloalkyl ring containing 1 or 2 heteroatoms independently chosen from N, S, and O.

[0019] R? and Re may be joined to form an optionally substituted 3- to 7- membered cycloalkyl ring or an optionally substituted 3- to 7-membered heterocycloalkyl ring containing 1 or 2 heteroatoms independently chosen from N, S, and O.

[0020] Rs is a C7-C״ saturated or unsaturated hydrocarbon chain that is (i) covalently bound to R7, where R7 is a methylene group or Rs is a C7-C1! saturated or unsaturated hydrocarbon chain that is (ii) covalently bound to an optionally substituted cycloalkyl ring formed by R7 and Re being joined to from a 3- to 7- membered optionally substituted cycloalkyl ring; and R« is hydrogen, C]־C6alkyl, or (C3-C7cycloalkyl)C0-C2alkyl;.

[0021] T is a tetrazole group attached via its carbon atom, or

T is a group of the formula:

O

[0022] Where, R9 is hydroxyl, amino, -COOH, -NR!0Rn, -OR12, -SR12, -NR10(S=O)Rn, -NR10SO2R11, -NR10SONR11R12, *NR10SO2NR11R12> -(C^OJORiq, -NRjofC^OjORn, orCONR10R11, orR9 is C1־C6alkyl, C2-C₆alkenyl, C₂-C6alkanoyl, (C₃-C7cycloalkyl)C0-C4alkyl, (Cj־C7cycloalkenyl)C0־C4alkyl, (C3-C7cycloalkyl)CH2SO2־, (C3-C7cycloalkyl)CH2SO2NR!0-, (heterocycloalkyl)C0-C4alkyl, (aryl)C₀-C2alkyl, or (5- to 10-membered heteroaryl)C0-C2alkyl, each of which is optionally substituted.

[0023] Or, R9 is a phosphonate of the formula

R13 ־־IVpA⁵

II ^R14

O where p is 0,1, or 2.

[0024] Or, R9 is Rj£XC0-C4alkyl־, where X is -(C=O)NH-, -NH(C=O). and Rx is aryl or heteroaryl, orRpis ~CH(RY)(C₃-C7cycloalkyl), -SO2CH(RY)(C3-C7cycloalkyl), orNR10SQ2CH(R.Y)(C3-C7cycloalkyl), where Ry is halogen or Ry is C!-C6alkyl, C2-C6alkanoyl, (C3-C7cycloalkyl)C0־C4alkyl, (C4-C7cydoalkenyl)C0-C4alkyl, (aryl)C0-C4alkyl, (aryl)CoWO 2008/008502

C₄alkoxy, (heterocycloalkyl)C0־C2alkyl, or (5- to 10-membered heteroaryl)Cb-C4alkyl, each of which is optionally substituted.

[0025] R_l0, R!1, and R₁₂ are independently at each occurrence hydrogen, or Ci-Ccalkyl, CrQalkenyt C₂-C6alkynyl, (aryl)C0־C₂alkyl, (C₃-C₇cycloalkyl)C₀־C₂alkyl, (C₃C₇cycloalkenyl)C0-C_2alkyl, (heterocycloalkyl)C0-C₂alkyl, or (5- to 10-membered heteroaryl)CoC₂alkyl, each of which is optionally substituted.

[0026] R13 is hydrogen or C!-C₂alkyl.

[0027] R!4 and R!_s are independently hydrogen, hydroxyl, or C!-C₂ alkyl.

[0028] n is 0,1, or 2.

[0029] M is hydrogen, halogen, hydroxyl, Ci-Cjalkyl, or C!-C₂alkoxy.

[0030] Y is absent, CR18&19, NR20, S, O, -O(C=O)(NR20)־־ NH(C=0)(NR.₂o)-, NH(S>=O)(NR20)־, or -O(C=O)-; or Y is taken together with one of J, L, or M to form a ring.

[0031 ] J is CH₂ or J is taken together with Y to form a 3- to 7- membered carbocyclic or heterocyclic ring, which ring is substituted with 0 or 1 or more substituents independently chosen from halogen, hydroxy, amino, cyano, Q-Cialkyl, C!-C₂alkoxy, C.-^alkoxy, trifluoromethyl, and trifluoromethoxy; when J is taken together with Y to form a ring Z may be absent.

[0032] L is CH2 or L is taken together with Y to form a 3- to 7- membered carbocyclic or heterocyclic ring, which ring is substituted with 0 or 1 or more substituents independently chosen from halogen, hydroxy, amino, cyano, Chalky!, C,-C₂alkoxy, C^alkoxy, trifluoromethyl, and trifluoromethoxy, when L is taken together with Ύ to form a ring Z may be absent

[0033] Z is (mono- or bicyclic aryl)Co־C₂a!kyl or (mono- or bicyclic heteroaryl)CoCialkyl, each of which Z is substituted with 0 or 1 or more substituents independently chosen from halogen, hydroxyl, amino, cyano, -CONH₂, -COOH, -SO^nR«, -(00)NR״R₁₂, NR״(C=־O)R1₂, C1-C4alkyl, C₂־C₄alkanoyl, C!-C4alkoxy, C_rC4alkylthio, mono- and di-C1C4alkylamino, C!־C4 alkyl ester, C1-C₂haloalkyl, and C1-C₂haloalkoxy, and 0 or 1 (C3C7cycloalkyl)C₀C₂alkyl, (phenyl)C₀-C₂alkyl, (phenyl)Co-C₂alkoxy, (5- or 6-membered heteroaryl)C&lt;j-C₂alkyl, ¢5- or 6-membered heteroaryl)C0-C₂alkoxy, naphthyl, indanyl, (5- or 6membered heterocycloalkyl)C₀־C₂alkyl, or 9- or 10-membered bicyclic heteroaryl, each of which is substituted with 0,1, or 2 substituents independently chosen from:

(c) halogen, hydroxyl, amino, cyano, nitro, -COOH, -CONH2, CHj(C=O)NH-, «NOH, C!-C4alkyl, C!-C4alkoxy, C1-C4hydroxyalkyl, mono- and di-C]-C4alkylamino, -NR8SO2R11, C(O)OR״, -NRgCORib -NR8C(O)OR״, trifluoromethyl, and trifluoromefhoxy, and (d) phenyl and 5- or 6-membered heteroaryl, each of which is substituted with 0 or 1 or more of halogen, hydroxyl, C!-C4alkyl, and C!-C₂alkoxy.

[0034] R!6 represents 0 to 4 substituents is independently chosen at from halogen, C!C₂alkyl, and C!-C2alkoxy.

[0035] Rib and R!9 are independently hydrogen, hydroxyl, halogen, C!-C2alkyl, C!C2alkoxy, CrCihaloalkyl, orC1-C2haloalkoxy.

[0036] Rao is hydrogen, Cj-C2alkyl, C1-C2haloalkyl, or C]-C₂haloalkoxy.

[0037] Certain compounds of Formula I disclosed herein exhibit good activity in an HCV replication assay, such as the HCV replicon assay set forth in Example 3, which follows. Preferred compounds of Formula I exhibit an EC5q of about 40 micromolar or less, or more preferably an EC50 of about 10 micromolar or less; or still more preferably an ECjq of about 5 nanomolar or less in an HCV replicon replication assay

DETAILED DESCRIPTION OF THE INVENTION Chemical Description and Terminology

[0038] Prior to setting forth the invention in detail, it may be helpful to provide definitions of certain terms to be used herein. Compounds of the present invention are described using standard nomenclature. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs. Unless clearly contraindicated by the context each compound name includes the free acid or free base form of the compound as well hydrates of the compound and all pharmaceutically acceptable salts of the compound. ^y

[0039] The term 4-amino-4-oxobutanoyl peptides encompasses all compounds that satisfy Formula I, including any enantiomers, racemates and stereoisomers, as well as all pharmaceutically acceptable salts of such compounds. The phrase a compound of Formula Γ’ includes all forms of such compounds, including salts and hydrates, unless clearly contraindicated by the context in which this phrase is used.

[0040] The terms “a” and “an” do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced item. The term “or” means “and/or. The terms “comprising”, “having, “including”, and “containing” are to be construed as open-ended terms (i.e., meaning “including, but not limited to). Recitation of ranges of values are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. The endpoints of all ranges are included within the range and independently combinable. AU methods described herein can be performed in a suitable order unless otherwise indicated herein or otherwise clearly contradicted by context The use of any and all examples, or exemplary language (e.g., “such as”), is intended merely to better illustrate the invention and does not pose a limitation on the scope of the invention unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the invention as used herein. Unless defined otherwise, technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs.

[0041] An “active agent” means a compound (including a compound of the invention), element, or mixture that when administered to a patient, alone or in combination with another compound, element, or mixture, confers, directly or indirectly, a physiological effect on the patient. The indirect physiological effect may occur via a metabolite or other indirect mechanism. When the active agent is a compound, then salts, solvates (including hydrates) of the free compound, crystalline forms, non-crystalline forms, and any polymorphs of the compound are included. Compounds may contain one or more asymmetric elements such as stereogenic centers, stereogenic axes and the like, e.g., asymmetric carbon atoms, so that the compounds can exist in different stereoisomeric forms. These compounds can be, for example, racemates or optically active forms. For compounds with two or more asymmetric elements, these compounds can additionally be mixtures of diastereomers. For compounds having asymmetric centers, all optical isomers in pure form and mixtures thereof are encompassed. In addition, compounds with carbon-carbon double bonds may occur in Z- and E-forms, with all ׳ isomeric forms of the compounds. In these situations, the single enantiomers, i.e., optically active forms can be obtained by asymmetric synthesis, synthesis from optically pure precursors, or by resolution of the racemates. Resolution of the racemates can also be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example a chiral HPLC column. All forms are contemplated herein regardless of the methods used to obtain them.

[0042] A dash that is not between two letters or symbols is used to indicate a point of attachment for a substituent. For example, -(CH₂)C₃-C₈cycloalkyl is attached through carbon of the methylene (CHj) group.

[0043] “Alkanoyl” indicates an alkyl group as defined herein, attached through a keto ((00)-) bridge. Alkanoyl groups have the indicated number of carbon atoms, with tire carbon of the keto group being included in the numbered carbon atoms. For example a C₂alkanoyl group is an acetyl group having the formula CH₃ (00)-.

[0044] A bond represented by a combination of a solid and dashed line, ie. , may be either a single or double bond.

[0045] “Alkyl” is a branched or straight chain saturated aliphatic hydrocarbon group, having the specified number of carbon atoms, generally from 1 to about 12 carbon atoms. The term C1-C₆alkyl as used herein indicates an alkyl group having from 1 to about 6 carbon atoms. Other embodiments include alkyl groups having from 1 to 8 carbon atoms, 1 to 4 carbon atoms or from 1 to 2 carbon atoms, e.g. C1־C8alkyl, C1-C4alkyl, and C!-C₂alkyl. When Co־Cn alkyl is used herein in conjunction with another group, for example, (aryl)C0-C4 alkyl, the indicated group, in this case aryl, is either directly bound by a single covalent bond (Co), or attached by an alkyl .chain having the specified number of carbon atoms, in this case from 1 to about 4 carbon atoms. Co-C_nalkyl is used in conjunction with heteroaryl, aryl, phenyl, cycloalkyl, and heterocycloalkyl, e.g5) ״- to 10-membered heteroaryl)C0-C2alkyl, (aryl)C₀-C2alkyl, (phenyl)C0C₂alkyl, (Cj-C7cycloalkyl)C0-C4alkyl, and (heterocycloalkyl)C0-C4alkyl. Examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, 3-methylbutyl, t-butyl, n-pentyl, and sec-pentyl.

[0046] “Alkenyl” indicates a straight or branched hydrocarbon chain comprising one or more unsaturated carbon- carbon double bonds, which may occur in any stable point along the chain. Alkenyl groups described herein have the indicated number of carbon atoms. E.g. C2C₆alkenyl indicates an alkenyl group of from 2 to about 6 carbon atoms. When no number of carbon atoms is indicated, alkenyl groups described herein typically have from 2 to about 12 carbon atoms, though lower alkenyl groups, having 8 or fewer carbon atoms, are preferred. Examples of alkenyl groups include ethenyl, propenyl, and butenyl groups.

[0047] “Alkynyl” indicates a straight or branched hydrocarbon chain comprising one or more carbon-carbon triple bonds, which may occur in any stable point along the chain. Alkenyl groups described herein have the indicated number of carbon atoms. E.g. C!-C6alkynyl indicates an alkynyl group of from 2 to about 6 carbon atoms. When no number of carbon atoms is indicated, alkynyl groups described herein typically have from 2 to about 12 carbon atoms, though lower alkynyl groups, having 8 or fewer carbon atoms, are preferred.

[0048] “Alkoxy” indicates ah alkyl group as defined above with the indicated number of carbon atoms attached through an oxygen bridge (-O-). Examples of alkoxy include, but are not limited to, methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, 2-butoxy, t-butoxy, n־pentoxy, 2pentoxy, 3- pentoxy, isopentoxy, neopentoxy, n-hexoxy, 2-hexoxy, 3-hexoxy, and 3methylpentoxy. When “Co-Cnalkoxy” is used in with another group, for example, (heteroaryl)C0־C4 alkoxy, the indicated group, in this case heteroaryl, is either attached via a covalently bound oxygen bridge (Coalkoxy), or attached by an alkoxy group having the specified number of carbon atoms, in thia case from 1 to about 4 carbon atoms, that is covalently bound to the group it substitutes via the alkoxy oxygen atom.

[0049] The term “alkylester” indicates an alkyl group as defined herein attached through an ester linkage. The ester linkage may be in either orientation, e.g. a group of the formula —O(C=O)alkyl or a group of the formula -(C“O)Oalkyl.

[0050] “Alkylthio” means alkyl-S-, where the alkyl group is an alkyl group as defined herein having the indicated number of carbon atoms and the point of attachment of the alkythio substituent is on the sulfur atom. An exemplary alkylthio group is methylthio.

[0051] “Aryl” indicates an aromatic group containing only carbon in the aromatic ring or rings. Such aromatic groups maybe further substituted with carbon or non-cafbon atoms or groups. Typical aryl groups contain 1 or 2 separate, fused, or pendant rings and from 6 to about 12 ring atoms, without heteroatoms as ring members. Where indicated aryl groups may be substituted. Such substitution may include fusion to a 5 to 7-membered saturated cyclic group that optionally contains 1 or 2 heteroatoms independently chosen from N, O, and S, to form, for example, a 3,4-m ethylenedioxy-phenyl group. Aryl groups include, for example, phenyl, naphthyl, including 1-naphthyl and 2-naphthyl, and bi-phenyl.

[0052] In the term “(aryl)alkyl,” aryl and alkyl are as defined above, and the point of attachment is on the alkyl group. “(Aryl)C0-C4alkyl” indicates an aryl group that is directly attached via a single covalent bond (aryl)Coalkyl or attached through an alkyl group having from 1 to about 4 carbon atoms. Examples of (aryl)alkyl groups include piperonyl and (phenyl)alkyl groups such as benzyl and phenylethyl. Similarly, the term “(a1yl)C0־C₄alkoxy” indicates an aryl group that is directly attached to the molecule it substitutes via an oxygen bridge, e.g. (aryl)C<>aIkoxy, or covalently bound to an alkoxy group having from 1 to 4 carbon atoms.

[0053] A “carbocyclic ring is a saturated, partially unsaturated, or aromatic cyclic group containing only carbon ring atoms. A “5- to 7-membered carbocyclic ring” has from 5 to 7 carbon ring atoms. Unless otherwise indicated, the carbocyclic ring may be attached to its pendant group at any carbon atom that results in a stable structure. When indicated the carbocyclic rings described herein may be substituted on any available ring carbon if the resulting compound is stable. Carbocyclic rings include, cycloalkyl groups, such as cyclopropyl and cyclohexyl; cycloalkenyl groups, such as cyclohexenyl, bridged cycloalkyl groups; and aryl groups, such as phenyl.

[0054] “Cycloalkyl” saturated hydrocarbon ring group, having the specified number of cartion atoms. Monocyclic cycloalkyl groups typically have from 3 to about 8 carbon ring atoms or from 3 to about 7 carbon ring atoms. Cycloalkyl substituents may be pendant from a substituted nitrogen or carbon atom, or a substituted carbon atom that may have two substituents may have a cycloalkyl group, which is attached as a spiro group. Examples of cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl as well as bridged or caged saturated ring groups such as norbomane or adamantane. Likewise “cycloalkenyl is a hydrocarbon ring group having the indicated number of carbon atoms and at least carbon-carbon double between ring carbon atoms.

[0055] The terms “(cycloalkyl)Co-Cnalkyl and “(cycloalkenyl) Co-Cnalkyl” indicate a substituent in which the cycloalkyl or cycloalkenyl and alkyl are as defined herein, and the point of attachment of the (cycloalkyl)alkyl group or (cycloalkenyl)alkyl group to the molecule it substitutes is either a single covalent bond, (Coalkyl) or on the alkyl group. (Cycloalkyl)alkyl encompasses, but is not limited to, cyclopropylmethyl, cyclohexylmethyl, and cyclohexylmethyl.

[0056] “Haloalkyl” indicates both branched and straight-chain alkyl groups having the specified number of carbon atoms, substituted with 1 or more halogen atoms, up to the maximum allowable number of halogen atoms. Exempt of haloalkyl include, but are not limited to, trifluoromethyl, difluoromethyl, 2-fluoroethyl, and penta-fluoroethyl.

[0057] ״Haloalkoxy” indicates a haloalkyl group as defined herein attached through an oxygen bridge (oxygen of an alcohol radical).

[0058] *Ή31ο” or “halogen” indicates any of fluoro, chloro, bromo, and iodo.

[0059] “Heteroaryl” indicates a stable monocyclic aromatic ring having the indicated number of ring atoms which contains from 1 to 3, or in some embodiments from 1 to 2, heteroatoms chosen from N, O, and S, with remaining ring atoms being carbon, or a stable bicyclic or tricyclic system containing at least one 5- to 7-membered aromatic ring which contains from 1 to 3, or in some embodiments from 1 to 2, heteroatoms chosen fromN, O, and S, with remaining ring atoms being carbon. Monocyclic heteroaryl groups typically have from 5 to 7 ring atoms. In some embodiments bicyclic heteroaryl groups are 9- to 10-membered heteroaryl groups, that is, groups containing 9 or 10 ring atoms in which one 5- to 7-member aromatic nng is fused to a second aromatic or non-aromatic ring. ‘״Tricyclic heteroaryl” groups contain three fused rings, at least one of which is a heteroaryl ring. When the total number of S and O atoms in the heteroaryl group exceeds 1, these heteroatoms are not adjacent to one another. It is preferred that the total number of S and O atoms in the hetcroaryl group is not more than 2. It is particularly preferred that the total number of S and O atoms in the aromatic heterocycle is not more than 1. Examples of heteroaryl groups include, but are not limited to, oxazolyl, pyranyl, pyrazinyl, pyrazolopyrimidinyl, pyrazolyl, pyridizinyl, pyridyl, pyrimidinyl, pynolyl, quinolinyl, tetrazolyl, thiazolyl, thienylpyrazolyl, thiophenyl, triazolyl, benzo[^oxazolyl, benzofhranyl, benzothiazolyl, benzothiophenyl, benzoxadiazolyl, dihydrobenzodioxynyl, furanyl, imidazolyl, indolyl, and isoxazolyl.

[0060] The term “heterocycloalkyl” indicates a saturated monocyclic group having the indicated number of ring atoms and containing from 1 to about 3 heteroatoms chosen from N, O, and S, with remaining ring atoms being carbon, or a saturated bicyclic ring system having at least one N, O, or S ring atom with the remaining atoms being carbon. Monocyclic heterocycloalkyl groups usually have from 4 to about 8 ring atoms. In some embodiments monocyclic heterocyloalkyl groups have from 5 to 7 ring atoms. Bicyclic heterocycloalkyl groups typically have from about five to about 12 ring atoms. Examples of heterocycloalkyl groups include morpholinyl, piperazinyl, piperidinyi, and pyrrolidinyl groups.

[0061] The term “(heterocycloalkyl)alkyl” indicates a saturated substituent in which the heterocycloalkyl and alkyl are as defined herein, and the point of attachment of the (heterocycloalkyl)alkyl group to the molecule it substitutes is on the alkyl group. This term encompasses, but is not limited to, piperidylmethyl, piperazinylmethyl, and pyrrolidinylmethyl.

[0062] The term “heterocyclic ring” indicates a saturated, partially unsaturated, or aromatic cyclic group having the indicated number of ring atoms, typically from 5 to 8 ring atoms, and containing from 1 to about 4 heteroatoms chosen from N, O, and S, with remaining ring atoms being carbon, or bicyclic saturated, partially unsaturated, or aromatic heterocylic or tricyclic ring system, containing at least 1 heteroatom in the multiple ring system chosen from N, O, and S and containing up' to about 4 heteroatoms independently chosen from N, O, and S in each ring of the multiple ring system. Bicyclic and tricyclic rings have the indicated number of ring atoms with bicyclic heterocyclic ring systems typically having from 7 to 11 ring atoms and tricyclic systems from 10 to 15 ring atoms. Examples of heterocyclic groups include, pyridyl, indolyl, pyrimidinyl, pyridizinyl, pyrazinyl, imidazolyl, oxazolyl, furanyl, thiophenyl, thiazolyl, triazolyl, tetrazolyl, isoxazolyl, quinolinyl, pyrrolyl, pyrazolyl, benz[&amp;]thiophenyl, isoquinolinyl, quinazolinyl, quinoxalinyl, thienyl, isoindolyl, dihydroisoindolyl, 5,6,7,8-tetrahydroisoquinoline, pyridinyl, pyrimidinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, pyrrolidinyl, moipholinyl, piperazin yl, piperidinyl, and pyrrolidinyl.

[0063] Additional examples of heterocyclic groups include, but are not limited to 1,1dioxo-thieno-tetrahydrothiopyranyl, 1,1-dioxothiochromanyl, 1,4-dioxanyl, 5-pteridinyl, tetrahydroindazolyl, azetidinyl, benzimidazolyl, benzisoxazinyl, benzodioxanyl, benzodioxolyl, benzofurazanyl, benzoisoxolyl, benzopyranyl, benzqpyrazolyl, benzotetrahydrofuranyl, benzotetrahydrothienyl, benzothiopyranyl, benzotriazolyl, benzoxazinyl, benzoxazolinonyl, benzoxazolyl, beta-carbolinyl, carbazolyl, carbolinyl, chromanonyl, chromanyl, cinnolinyl, coumarinyl, dihydroazetidinyl, dihydrobenzisothiazinyl, dihydrobenzisoxazmyl, dihydrobenzodioxinyl, dihydrobenzofuranyl, dihydrobenzoimidazolyl, dihydrobenzothiophenyl, dihydrobenzoxazolyl, dihydrocoumarinyl, dihydroindoljd, dihydroisocoumarinyl, dihydroisooxazolyl, dihydroisoquinolinonyl, dihydroisothiazolyl, dihydrooxadiazolyl, dihydropyrazinyl, dihydropyrazolyl, dihydropyridinyl, dihydropyrimidinyl, dihydropyrrolyl, dihydroquinolinonyl, dihydroquinolinyl, dihydrotetrazolyl, dihydrothiadiazolyl, dihydrothiazolyl, dihydrothienyl, dihydrotriazolyl, hexahydroazepinyl, imidazopyrazinyl, imidazopyridazinyl, imidazopyridinyl, imidazopyridyl, imidazopyrimidinyl, imidazothiadiazolyl, imidazothiazolyl, imidazothiophenyl, indolinyl, indoltzinyl, isobenzotetrahydrofuranyl, isobenzotetrahydrothienyl, isobenzothienyl, isochromanyl, isocoumarinyl, isoindolinonyl, isbindolmyl, isoquinolyl, isothiazolyl, isoxazolyl, methylenedioxybenzyl, naphthyridinyl, oxadiazolyl, oxazolopyridinyl, oxazolyl, oxetanyl, oxopiperidinyl, oxopyrazolyl, oxopyridinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, purinyl, pyrazinyl, pyrazolopyrazinyl, pyrazolopyridazinyl, pyrazolopyridyl, pyrazolopyrimidinyl, pyrazolothiophenyl, pyrazolotriazinyl, pyridazinyl, pyridopyridinyl, quinazolinjd, quinolinyl, quinoxalinyl, tetrahydrofuranyl, tetrahydroimidazopyrazinyl, tetrahydroimidazopyridazinyl, tetrahydroimidazopyridinyl, tetrahydroimidazopyrimidyl, tetrahydroisoquinolinyl, tetrahydropyranyl, tetrahydropyrazolopyrazinyl, tetrahydropyrazolopyridinyl, tetrahydropyrazolopyrimidyl, tetrahydro quinolinyl, tetrahydrothienyl, tetrahydrotriazolopyrimidyl, tetrahydrotriazolopyrazinyl, tetrahydrotriazolopyridazinyl, tetrahydrotriazopyridinyl, tetrazolopyridyl, tetrazolyl, thiadiazolyl, thieno-tetrahydrothiopyranyl, thienyl, thiochromanyl, triazinyl, triazolopyrazinyl, triazolopyridazinyl, triazolopyridyl, triazolopyrimidinyl, triazolothiophenyl, and where possible, N-oxides thereof.

[0064] “Hydroxyalkyi is an alkyl group having the indicated number of carbon atoms and substituted with at least one hydroxyl substituent Where indicated, the hydroxyalkyl group may be further substituted.

[0065] The term “mono- and/ or di-alkylamino” indicates secondary or tertiary alkyl amino groups, wherein the alkyl groups are independently chosen alkyl groups, as defined herein, having the indicated number of carbon atoms. The point of attachment of the alkylamino group is on the nitrogen. Examples of mono- and di-alkylamino groups include ethyl amino, Him ethylamino, and methyl-propyl-amino.

[0066] “Mono- and/ or di-alkylcarboxamide” indicates a mono-alkylcarboxamide group of formula (alkyl l)-NH-(OO)- or a dialkylcarboxamide group of the formula (alkyl l)(alky!2)N-(C=O)- in whioh the point of attachment of the mono- or dialkylcarboxamide substituent to the molecule it substitutes is on the carbon of the carbonyl group. The term “mono and/ or dialkylcarboxamide״ also includes groups of the formula (alkyl 1)(C=O)NH- and (alkyll)(C=O) (alkyl2)N- in which the point of attachment is the nitrogen atom. The groups alkyl 1 and alkyl2 are independently chosen alkyl groups having the indicated number of carbon atoms.

[0067] *Oxo, means a keto group (C=O). An oxo group that is a substituent of a nonaromatic carbon atom results in a conversion of -CH2— to -C(=O)-. An oxo group that is a substituent of an aromatic carbon atom results in a conversion of —CH— to -C(=O)- and a loss of aromaticity.

[0068] The term ^,,substituted”, as used herein, means that any one or more hydrogens on the designated atom or group is replaced with a selection from tire indicated group, provided that the designated atom's normal valence is not exceeded. When the substituent is oxo (i.e., “O) then 2 hydrogens on the atom are replaced. When an oxo group substitutes aromatic moieties, the corresponding partially unsaturated ring replaces the aromatic ring. For example a pyridyl group substituted by oxo is a pyndone. Combinations of substituents and/or variables are permissible only if such combinations result in stable compounds or useful synthetic intermediates. A «table compound or stable structure is meant to imply a compound that is sufficiently robust to survive isolation from a reaction mixture, and subsequent formulation into an effective therapeutic agent Unless otherwise specified substituents are named into the core structure. For example, it is to be understood that when (cycloalkyl)alkyl is listed as a possible substituent the point of attachment of this substituent to the core structure is in the alkyl portion.

[0069] Suitable groups that may be present on a “substituted” position include, but are not limited to, e.g., halogen; cyano; hydroxyl; nitro; azido; alkanoyl (such as a C2-C6 alkanoyl group such as acyl or the like); carboxamido; alkyl groups (including cycloalkyl groups, having 1 to about 8 carbon atoms, or 1 to about 6 carbon atoms); alkenyl and alkynyl groups (including groups having one or more unsaturated linkages and from 2 to about 8, or 2 to about 6 carbon atoms); alkoxy groups having one or more oxygen linkages and from 1 to about 8, or from 1 to about 6 carbon atoms; aryloxy such as phenoxy־, alkylthio groups including those having one or more thioether linkages and from 1 to about 8 carbon atoms; or from 1 to about 6 carbon atoms; alkylsulfinyl groups including those having one or more sulfinyl linkages and from 1 to about 8 carbon atoms, or from 1 to about 6 carbon atoms; alkylsulfonyl groups including those having one or more sulfonyl linkages and from 1 to about 8 carbon atoms, or from 1 to about 6 carbon atoms; aminoalkyl groups including groups having one or more N atoms and from 1 to about 8, or from 1 to about 6 carbon atoms; aryl having 6 or more carbons and one or more rings, (e.g., phenyl, biphenyl, naphthyl, or the like, each ring either substituted or unsubstituted aromatic); arylalkyl having 1 to 3 separate or fused rings and from 6 to about 18 ring carbon atoms, with benzyl being an exemplary arylalkyl group; arylalkoxy having 1 to 3 separate or fused rings and from 6 to about 18 ring carbon atoms, with benzyloxy being an exemplary arylalkoxy group; or a saturated, unsaturated, or aromatic heterocyclic group having 1 to 3 separate or fused rings with 3 to about 8 members per ring and one or more N, O or S atoms, e.g. coumarinyl, quinolinyl, isoquinolinyl, quinazolinyl, pyridyl, pyrazinyl, pyrimidinyl, furanyl, pyrrolyl, thienyl, thiazolyl, tnazinyl, oxazolyl, isoxazolyl, imidazolyl, indolyl, benzofuranyl, benzothiazolyl, tetrahydrofuranyl, tetrahydropyranyl, piperidinyl, morpholinyl, piperazinyl, and pyrrolidinyl. Such heterocyclic groups may be further substituted, e.g. with hydroxy, alkyl, alkoxy, halogen and amino

[0070] A “vinyl” group is a substituent of the formula *HO ^:.

[0071] A “dosage form” means a unit of administration of an active agent. Examples of dosage forms include tablets, capsules, injections, suspensions, liquids, emulsions, creams, ointments, suppositories, inhalable forms, transdermal forms, and the like.

[0072] “Pharmaceutical compositions” are compositions comprising at least one active agent, such as a compound or salt of Formula I, and at least one other substance, such as a carrier, excipient, or diluent Pharmaceutical compositions meet the U.S. FDA’s GMP (good manufacturing practice) standards for human or non-human drugs.

[0073] “Pharmaceutically acceptable’salts” includes derivatives of the disclosed compounds in which the parent compound is modified by making inorganic and organic, nontoxic, acid or base addition salts thereof. The salts of the present compounds can be synthesized from a parent compound that contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting free acid forms of these compounds with a stoichiometric amount of the appropriate base (such as Na, Ca, Mg, or K hydroxide, carbonate, bicarbonate, or the like), or by reacting free base forms of these compounds with a stoichiometric amnunt of the appropriate acid. Such reactions are typically earned out in water or in an organic solvent, or in a mixture of the two. Generally, non-aqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred, where practicable. Salts of the present compounds further include solvates of the compounds and of the compound salts.

[0074] Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. The pharmaceutically acceptable salts include the conventional non-toxic salts and the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. For example, conventional nontoxic acid salts include those derived from inorganic acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like; and the salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, mesylic, esylic, besylic, sulfanilic, 2-acctoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, HOOC-(CH2)n-COOH where n is 0-4, and the like. Lists of additional suitable salts may be found, e.g., in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., p. 1418 (1985).

[0075] The term “carrier” applied to pharmaceutical compositions of the invention refers to a diluent, excipient, or vehicle with which an active compound is provided.

[0076] A “pharmaceutically acceptable excipient” means an excipient that is useful in preparing a pharmaceutical composition that is generally safe, non-toxic and neither biologically nor otherwise undesirable, and includes an excipient that is acceptable for veterinary use as well as human pharmaceutical use. A “pharmaceutically acceptable excipient” as used in the present application includes both one and more than one such excipient.

[0077] A “patient” is a human ornon-human animal in need of medical treatment. Medical treatment can include treatment of an existing condition, such as a disease or disorder, prophylactic or preventative treatment, or diagnostic treatment. In some embodiments the patient is a human patient.

[0078] “Prodrug” means any compound that becomes compound of the invention when administered to a mammalian subject, e.g., upon metabolic processing of the prodrug. Examples of prodrugs include, but are not limited to, acetate, formate and benzoate and like derivatives of functional groups (such as alcohol or amine groups) in the compounds of the invention.

[0079] “Providing” means giving, administering, selling, distributing, transferring (for profit or not), manufacturing, compounding, or dispensing.

[0080] “Providing a compound of Formula I with at least one additional active agent” means the compound of Formula I and the additional active agent(s) are provided simultaneously in a single dosage form, provided concomitantly in separate dosage forms, or provided in separate dosage forms for ariminisfratinn separated by some amount of time that is within the fimpt in which both the compound of Formula I and the at least one additional active agent are within the blood stream of a patient The compound of Formula I and the additional active agent need not be prescribed for a patient by the same medical care worker. The additional active agent or agents need not require a prescription. Administration of the compound of Formula I or the at least one additional active agent can occur via any appropriate route, for example, oral tablets, oral capsules, oral liquids, inhalation, injection, suppositories or topical contact.

[0081] ‘Treatment,” as used herein includes providing a compound of Formula I and at least one additional active agent sufficient to: (a) prevent a disease or a symptom of a disease from occurring in a patient who may be predisposed to the disease but has not yet been diagnosed as having it (e.g. including diseases that may be associated with or caused by a primary disease (as in liver fibrosis that can result in the context of chronic HCV infection); (b) inhibiting die disease, i.e. arresting its development; and (c) relieving the disease, i.e., causing regression of the disease. *Treating” and “treatment” also means providing a therapeutically effective amount of a compound of Formula I and at least one additional active agent to a patient having or susceptible to a hepatitis C infection.

[0082] A “therapeutically effective amount” of a pharmaceutical combination of this invention means an amount effective, when administered to a patient, to provide a therapeutic benefit such as an amelioration of symptoms, e.g., an amount effective to decrease the symptoms of a hepatitis C infection. For example a patient infected with a hepatitis C virus may present elevated levels of certain liver enzymes, including AST and ALT. Normal levels of AST are from 5 to 40 units per liter of serum (the liquid part of the blood) and normal levels of ALT are from 7 to 56 units per liter of serum. A therapeutically effect amount is thus an amount sufficient to provide a significant reduction in elevated AST and ALT levels or an amount sufficient to provide a return of AST and ALT levels to the normal range. A therapeutically effective amount is also an amount sufficient to prevent a significant increase or significantly reduce the detectable level of virus or viral antibodies in the patient’s blood, serum, or tissues. One method of determining treatment efficacy includes measuring HCV RNA levels by a convention method for determining viral RNA levels such as the Roch TaqMan assay. In certain preferred embodiments treatment reduces HCV RNA levels below the

[0083] the limit of quantitation (30 lU/niL, as measured by the Roche TaqMan(R) assay) or more preferably below the limit of detection (10 lU/mL, Roche TaqMan).

[0084] A significant increase or reduction in the detectable level of virus or viral antibodies is any detectable change that is statistically significant in a standard parametric test of statistical significance such as Student’s T-test, where p &lt; 0.05.

[0085] Chemical Description

[0086] Formula I includes all subfonnulae thereof. In certain situations, the compounds of Formula I may contain one or more asymmetric elements such as stereogemc centers, stereogenic axes and the like, e.g. asymmetric carbon atoms, so that the compounds can exist in different stereoisomeric forms. These compounds can be, for example, racemates or optically active forms. For compounds with two or more asymmetric elements, these compounds can additionally be mixtures of diastereomers. For compounds having asymmetric centers, it should be understood that all of the optical isomers and mixtures thereof are encompassed. In addition, compotrnrig with carbon-carbon double bonds may occur in Z- and E-forms, with all isomeric forms of the compounds being included in the present invention. In these situations, single enantiomers, i.e., optically active forms, can be obtained by asymmetric synthesis, synthesis from optically pure precursors, or by resolution of the racemates. Resolution of the racemates can also be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example using a chiral HPLC column.

[0087] Where a compound exists in various tautomeric forms, the invention is not limited to any one of the specific tautomers, but rather includes all tautomeric forms.

[0088] The present invention is intended to include all isotopes of atoms occurring in the present compounds. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example, and without limitation, isotopes of hydrogen include tritium and deuterium and isotopes of carbon include ¹¹C, ¹³C, and ^״C.

[0089] Certain compounds are described herein using a general formula that includes variables, e.g. R!-R₉, R16, R18, R19, π» M. ^Y> ^{acd z}- Unless otherwise specified, each variable within such a formula is defined independently of other variables. Thus, if a group is said to be substituted, e.g. with 0-2 R*, then the group may be substituted with up to two R* groups and R* at each occurrence is selected independently from the definition of R*. Also, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds.

[0090] In addition to compounds of Formula I as described above, the invention also includes compounds of Formula I in which one or more of the following conditions is met forth© variables R!-R9, R!6, R18, R״, η. Μ, Y, and Z. The invention includes compounds of Formula I that carry any combination of the variable definitions set forth below that results in a stable compound.

<img file="IL196256A_D0003.tif" />

The R! and R2 variables

[0091] In certain embodiments the variables R! and R2 cany one of the definitions set forth below.

[0092] (1) (2) R] and Ri are joined to form a 5- to 7- membered heterocyloalkyJ ring containing 1 or 2 heteroatoms independently chosen from N, O, and which ring is optionally fused to a 5־ or 6-membered heteroaryl group (e. g. phenyl or pyridyl) to form a bicyclic ring system, each of which 5- to 7- membered heterocycloalkyl ring is optionally substituted.

[0093] (2) R! and Ra are joined to fonn a pyrrolidine, piperidine, or piperazine ring or a piperazine ring fused to a phenyl, each of which is optionally substituted with 0 to 3 substituents independently chosen from A- and AB- where A is halogen, hydroxyl, amino, cyano, -CONH2, COOH, C!-C4alkyl, C₂־C4alkanoyl, C!-C4alkoxy, Ct-Ctalkylthio, mono- or di-C₁-C4alkylamino, C1-C4alkylester, Ci-C^alkyiesteramine, mono- or di-C1־C4alkylcarboxamide, C!-C2haloalkyl, or Ci-Cjhaloalkoxy; and B is C!-C4alkyl.

[0094] (2) R! and R2 are joined to form a pyrrohdine, piperidine, or piperazine ring or a pipemrina ring fused to a phenyl, each of which is optionally substituted with 0 to 2 substituents independently chosen from halogen, hydroxyl, amino, CONH2, -COOH, C!-C2alkyl, and C!C₂alkoxy.

[0095] (3) R! is C!-C₄O(C=O)-, Cj-C4(O=O)WO 2008/008502

<img file="IL196256A_D0004.tif" />

[0096] (4) R2 is C!-C6alkyl or C3־C7cycloallcyl.

[0097] (5) R2 is hydrogen.

The variables R3 to Rg

[0098] In certain embodiments the variables R3 to Rg cany one of the definitions set forth below.

[0099] (1) R3, R4, R5, Ri, R7, and R8 are independently (a) hydrogen, or (b) Cj־C₄alkyl or (C3-C7cycloalkyl)C0-C4aUcyl, each of which is substituted with 0 to 3 substituents independently chosen from halogen, hydroxyl, amino, cyano, -CONH2, -COOH, C1-C4aikyl, C2WO 2008/008502

C₄alkaD0yl, C!-C₄alkoxy, C;-C4alkylthio, mono and di-C1-C4alkylamino, C!-C2haloalkyl, and C]-C2haloalkoxy.

[00100] (2) R3, R4, Rs, R6, R7, and R8 are independently hydrogen, C!-C4alkyl, or (C3-C7cycloalkyl)C0-C4alkyl.

[0100] (3) R3, R4, R5, R6, R7. and R« are independently hydrogen or methyl.

[0101] (4) Any of R3 and R4 and/ or Rs and Re, and/ or R? and Rs are joined to form a 3to 7- membered cycloalkyl ring or 3- to 7-membered heterocycloalkyl ring containing 1 or 2 heteroatoms independently chosen from N, S, and O, each of which ring, is substituted with 0 to 2 substituents independently chosen from halogen, hydroxyl, amino, cyano, vinyl, C1-C₂alkyl, C!C2alkoxy, trifluoromethyl, and trifluoromethoxy.

[0102] (5) Any of R₃ and R4 and/ or R₅ and R6, and/ or R7 and Rs are joined to form a 3to 7 membered cycloalkyl ring or 5- to 6-membered heterocycloalkyl ring containing 1 or 2 heteroatoms independently chosen fromN, S, and O, each of which ring is substituted with 0 to 2 substituents independently chosen from halogen, hydroxyl, C!-C2alkyl, and C!-C2alkoxy.

[0103] (6) R3, R4, and R&amp; are independently hydrogen or methyl;

Rs is C1-C₆alkyl, C2-C₆alkenyl, (C₃-C₇cycloalkyl)C0-C4alkyl, (C₃-C7cycloaIkenyl)C₀C4alkyl, (heterocycloalkyl)Co-C₄alkyl, C₂-C₆alkanoyl, or mono- or di- C1-C₆alkylamino; and

R₇ and R8 are joined to form an optionally substituted 3- to 7- membered cycloalkyl ring or an optionally substituted 3- to 7-membered heterocycloalkyl ring containing 1 or 2 heteroatoms independently chosen from N, S, and O.

[0104] (7) Rs is C1-C₆alkyl or (C₃-C7cycloalkyl)C0-C4alkyl; and R7 and Rg are joined to form a cyclopropyl ring, which is unsubstituted or substituted with 1 or 2 C!-C6alkyl or C2Cgalkenyl.

[0105] (8) The invention includes compounds in which R3 and R4 are hydrogen, Rs is hydrogen, R$ is t-butyl, and R7 and R8 are joined to form a cyclopropyl group substituted by a vinyl group. For example the invention includes compounds of Formula Π

<img file="IL196256A_D0005.tif" />

<img file="IL196256A_D0006.tif" />

(Formula Π)

[0106] (9) R.3, R4, R6, and Re are independently hydrogen or methyl; and Rs is a C7-C״ saturated or unsaturated hydrocarbon chain that is covalently bound to R7, where R7 ,is a methylene group. For example the invention includes compound of Formula ΙΠ

<img file="IL196256A_D0007.tif" />

(Formula ΓΠ) where D is an alkyl or alkenyl group having 6 to 10 carbon atoms.

[0107] (10) The invention also includes compounds in which R₃ and R4 are both hydrogen, and R5 is covalently bound to R₇ via an 8-membered mono-unsaturated hydrocarbon chain. For Example in the invention includes compounds of Formula (IV)

<img file="IL196256A_D0008.tif" />

O ^r6 (Formula IV).

[0108] (11) R3> R4> and ^are independently hydrogen or methyl; and Rs is a C7-C11 saturated or unsaturated hydrocarbon chain that is covalently bound to an optionally substituted cycloalkyl ring formed by R?and Rg being joined to from a 3- to 7 membered optionally substituted cycloalkyl ring. For example the invention includes compounds and salts of Formula V

<img file="IL196256A_D0009.tif" />

(Formula V) where D is an alkyl or alkenyl group having 6 to 10 carbon atoms.

[0109] (12) The invention also includes compounds and salts in which R3 and R4 are both hydrogen and Rs is a 7-carbon mono-unsaturated hydrocarbon chain that is covalently bound to a substituted cycloalkyl ring formed by R? anti Rg. For example the invention includes compounds and salts of Formula VI and VI-A:

<img file="IL196256A_D0010.tif" />

O ^rB (Formula VI)

<img file="IL196256A_D0011.tif" />

(Formula VI-A).

The R9 variable

[0110] In certain embodiments the R9 variable carries one of the definitions set forth below.

O &lt;A

[0111 ] (1) T is a group of the formula “ and R9 is hydroxyl, amino, -COOH, NR!0R!1, -OR12. -SR!2, -NR10(S=O)Rh,

-NRjoSChRu, -NR10SONR11R12, -NR10SO2NR11R12, -(C־=O)OR10, -NR!0(C=O)OR11, or

-CONR10R11[0112] (2) R9 is hydroxyl, amino, -COOH, -NR10R11,-OR12, -NR10SO2R11» ־ (C-OJORio, or-CONRioRu[0113] (3) R₉ is C1-C₆alkyl, C₂-C₆alkenyl, C₂-C6alkanoyl, (C3־C7cycloalkyl)C₀-C₄alkyl, (C₃-C7cycloalkenyl)C0-C4alkyl, (heterocycloalkyI)C0-C₄alkyl, (aryl)C0-C2alkyl, or (5־ to 10mf-mbpred heteroaryl)Co-C₂alkyl, each of which is substituted with 0 or 1 or more substituents independently chosen from halogen, hydroxyl, amino, cyano, oxo -COOH, .CONH2, oxo, Ci-Cialkyl, C!-C₄alkoxy, C₂-C₄alkanoyi, C!-C4alkylthio, mono- and di- C!C₄alkylamino, C1־C4alkylester, mono- and di- C₁-C₄alkylcarboxamide, C1-C₂haloalkyl, and C!Czhaloalkoxy.

[0114] (4) R9 is C1-C6alkyl, C2־C6alkenyl, C2-C_fialkanoyl, (C₃-C₇cycioalkyl)Co־C₄alkyl, (C3־C7cycloaLkenyl)C0־C4alkyl, (heterocycloalkyl)C0־C4alkyl, (aryl)C0־C2alkyl, or (5- to 10membered heteroaryl)C0-C₂alkyl each of which is substituted with 0 or 1 or more substituents independently chosen from halogen, hydroxyl, amino, cyano, oxo -COOH, -CONH2, C1-C4alkyl, Cj-C-jalkoxy, mono- and di-C1־C₄alkyIamino, Cj-C₂haloalkyl, and C!C2haloalkoxy.

[0115] (5) Rj is a phosphonate of the formula

R13 “ י P“^r14

O where p is 1 or 2 and Rn-Ris carry the values set forth in the “Summary of the Invention” section.

[0116] (6) R₉ is RxXC1-C4alkyl-, where X is -(OO)NH-, -NH(C=O). and Rx is phenyl or pyridyl.

[0117] (7) R9 is -CH(RY)(C3-C₇cycloalkyl), -SO₂CH(R_Y)(C₃-C₇cycloalkyl), or -NR10SO2CH(RY)(C₃-C7cycloalky)), where Ry is halogen, or Ry is C1-C₂alkyl, C₂-C₆alkanoyl, (C3-C7cycloalkyl)C0-C2alkyl, (phenyl)C0-C4alkyl, (phenyl)Co-C₄alkoxy, (heterocycloalkyl)C₀C₂alkyl, or (5- to 10-membered heteroaryl)C0-C4alkyl, each of which is substituted with 0 or 1 or more substituents independently chosen from halogen; hydroxyl, amino, cyano, oxo -COOH, -CONH2, oxo, C!-C4alkyl, C1-C4alkoxy, mono- and di-Ci-C4alkylamin□, C1־C2haloalkyl, and CiC₂haloalkoxy.

[0118] (8) R9 is -NR10SO2R11. In some embodiments R!o is hydrogen or methyl and R״ is cyclopropyl.

The Rio, Rh, and Rjj variables

[0119] In any of the above definitions of R9 the variables R!o, R11> and R!2 may cany the definition set forth for Formula I in the “Summary of the Invention” section. Rio, Rn, and R״ may also carry any of definitions that follow.

[0120] (1) Rio, Rn! and R!2 arc independently C!-C6alkyl, C2-C6alkenyl, C₂-C6alkynyl, (C₃-C₇cycIoalkyl)C0-C4alkyl, (heterocycloalkyl)C0-C4alkyl, (phenyl)C₀־C₂alkyl, (naphthyl)C₀C₂alkyl, or (5- to 10-membered heteroaryl)Co-C₂alkyl, each of which is substituted with 0 to 3 substituents independently chosen from halogen, hydroxyl, amino, cyano, oxo -COOH, -CONH₂, oxo, C!-C4alkyl, C!-C4alkoxy, C₂-C4alkanoyl, C1-C₄alkylthio, mono- and di- C1-C4alkylamino, C ] -(־!^ alkyl ester, mono- and di- C1-C4alkylcarboxem1de, C!-C₂haloalkyl, and C1-C₂haloalkoxy.

[0121] (2) Rio, Rn, and Rj2 are independently hydrogen, or C1-C6alkyl, C₂-C6alkenyl, C₂-C₆alkynyl, (C₃-C₇cycloalkyl)Co-C₂alkyl, (heterocycloalkyl)C0-C₂alkyl, (phenyi)Co-C₂alkyl, or (5- to 6-membered monocyclic heteroaryl)C0־C₂alkyl, each of which is substituted with 0 to 3 substituents independently chosen from halogen, hydroxyl, oxo, C]-C₂alkyl, C!-C2alkoxy, trifluoromethyl, and trifluoromethoxy.

[0122] (3) R!o, R11, and R!₂ are independently hydrogen or C]-C6alkyl.

The Y,M,J,L, and Z variables

[0121] The invention includes compounds and salts of Formulae I to VI in which Y, M, and Z cany the variables set forth below. Y, M, and Z include the variables n, R!e, end R19.

[0122] (1) Y is absent, CH₂, O, or -O(C=O>־ and n is 0.

[0123] (2) Y is O and n is 0.

[0124] (3) Y is absent, CH!, O, or -O(C=O)-, and R!_a and R!9 are independently hydrogen or methyl, and n is 1.

[0125] (4) The invention includes compounds and salts in which n is 1.

[0126] (5) M is hydrogen.

[0127] (6) J and L are both CH₂.

[0128] (7) Z is 1-naphthyl, 2-napthyl,

<img file="IL196256A_D0012.tif" />

<img file="IL196256A_D0013.tif" />

Br

<img file="IL196256A_D0014.tif" />

<img file="IL196256A_D0015.tif" />

<img file="IL196256A_D0016.tif" />

<img file="IL196256A_D0017.tif" />

F

<img file="IL196256A_D0018.tif" />

<img file="IL196256A_D0019.tif" />

<img file="IL196256A_D0020.tif" />

<img file="IL196256A_D0021.tif" />

<img file="IL196256A_D0022.tif" />

<img file="IL196256A_D0023.tif" />

<img file="IL196256A_D0024.tif" />

<img file="IL196256A_D0025.tif" />

<img file="IL196256A_D0026.tif" />

<img file="IL196256A_D0027.tif" />

<img file="IL196256A_D0028.tif" />

<img file="IL196256A_D0029.tif" />

<img file="IL196256A_D0030.tif" />

<img file="IL196256A_D0031.tif" />

σνν

<img file="IL196256A_D0032.tif" />

<img file="IL196256A_D0033.tif" />

[0129] (8) Z is a group of the formula

R23

<img file="IL196256A_D0034.tif" />

[0130] Within the above chemical formula X1-X5, G1-G4, and R21-R24 carry the following definitions.

[0131] Xi, X2, X3, X4, and Xj are independently N or CH and no more than two of X! X5 areN.

[0132] G], G_2j G3, and G₄ are independently CH₂, O, S, or NK24> wherein no more than two of G! to G4 are other than hydrogen.

[0133] R21 represents from 0 to 3 groups independently chosen from halogen, hydroxyl, amino, cyano, -CONH₂, -COOH, C_I־C₄alkyl, C₂-C4alkanoyl, C!-C4alkoxy, C!-C4alkylthio, mono- and di-C1-C4alkylamino, C!-C₂haloalkyl, and C!-C₂haloalkoxy.

[0134] R22 is hydrogen, halogen, hydroxyl, amino, cyano, -CONH2, -COOH, C!־C4alkyl, Cj-r^llfanoy], C1-C4alkoxy, C1-C4alkylthio, mono- and di-C1-C₄alkylamino, C1-C4alkylester, C1-C₂haloalkyl, and C_rC₂haloalkoxy, or

[0135] R32 is (C3-C7cycloalkyl)C0-C₂alkyl, (phenyl)C₀־C2alkyl, (phenyl)Co-C₂alkoxy, (5or 6-membered heteroaryl)C0־C2alkyl, (5- or 6-membered heteroaryl)Co־C₂alkoxy_> naphthyl, indanyl, (5- or 6-membered heterocycloalkyl)Co-C₂alkyl, or 9- or 10 membered bicyclic heteroaryl, each of which is substituted with 0,1, or 2 substituents independently chosen from (c) halogen, hydroxyh amino, cyano, nitro, -COOH, -CONH₂, CH3(C=O)NH-, C!C4alkyl, C!־C4alkoxy, Cj-C4hydroxyalkyl, mono- and di-C1-C4alkylamino, -NR8SO2R11, -C(O)OR11, -NReCORji, -NRjfC(O)OR!1, trifluoromethyl, trifluoromethoxy, and (d) phenyl and 5- or 6-membcred heteroaiyl, each of which is substituted with 0 or 1 or more of halogen, hydroxyl, Ci-C^alkyl, C!-C₂alkoxy.

[0136] R23 is 0 to 2 substituents independently chosen from halogen, hydroxyl, C!C₂alkyl, and C!-C₂alkoxy.

[0137] R24 is independently chosen at each occurrence from hydrogen and C!-C₂alkyl.

[0138] In certain embodiments R22 is

<img file="IL196256A_D0035.tif" />

<img file="IL196256A_D0036.tif" />

<img file="IL196256A_D0037.tif" />

[0139] (9) Z is a group of the formula

<img file="IL196256A_D0038.tif" />

<img file="IL196256A_D0039.tif" />

[0140] Within this embodiment X1-X5 and R21-R23 cany the following definitions.

[0141] X|, X2, X3, and X4, are independently N or CH and no more than two of X!-X4 areN.

[0142] Rai represents from 0 to 3 groups independently chosen from halogen, hydroxyl, amino, cyano, -CONH2, -COOH, C1-C4alkyl,־C2*C4alkanoyl, C!-C4alkoxy, C]-C4alkylthio, mono- and di-C|-C4alkylamino, C!-C2haloalkyl, and C!-C2haloalkoxy.

[0143] R22 is hydrogen, halogen, hydroxyl, amino, cyano, -CONHz, -COOH, C!-C4alkyl, C2־C4alkanoyl, C!-C4alkoxy, C1-C4alkylthio, mono- and di-C_rC4alkylamino, C1־C4alkylester, C!-C2haloalkyl, and C1-C2haloalkoxy.

[0144] Or, R22 is (C3-C7cyeloalkyl)C0C2alkyl, (phenyl) C0־C2alkyl, (phenyl)C0-C2alkoxy, (5- or 6-membered heteroaryl)C0-C2alkyl, (5- or 6-membered heteroaryl)C0-C2alkoxy, naphthyl, indanyl, (5- or 6-membered heterocycloalkyl)CcC₂alkyl, or 9- or 10 membered bicyclic heteroaryl, each of which is substituted with 0, 1, or 2 substituents independently chosen from (c) halogen, hydroxyl, amino, cyano, nitro, -COOH, -CONH2, CH3(C=־O)NH־, C!־ r.^UryL C|-C4alkoxy, C1-C4hydroxyalkyl, mono- and di-C1-C4alkylamino, -NR8SO2R11, ־ C(O)ORj1, -NRgCORu, -NRsC(O)OR״, trifluoromethyl, and trifluoromethoxy, and (d) phenyl and 5- or 6-membered heteroaryl, each of which is substituted with 0 or 1 or more of halogen, hydroxyl, C!-C4alkyl, C!־C2 alkoxy.

[0145] (10) Z is a group of the formula ¾1

<img file="IL196256A_D0040.tif" />

«/yv wherein X!-X₅ and RarRa carry the definitions set forth for embodiment (7), immediately above.

[0146] (9) Z is a quinoline of the formula

<img file="IL196256A_D0041.tif" />

wherein X!-X_s and R21-R22 cany the definitions set forth for embodiment (7).

[0147] Or in certain embodiments, R2j represents a substituent at the 7-position of the quinoline, and 0 to 2 additional substituents independently chosen from halogen, hydroxyl, amino, cyano, -CONH₂, -COOH, C!-C4alkyl, C₂-C4alkanoyl, C1-C₄alkoxy, mono- and di-C!C4alkylamino, Cj-C^haloalkyl, and C!-C₂haloalkoxy; and Ra is (phenyl)C₀ -C₂alkyl or (pyridyl)C0-C₂aikyl, each of which is substituted with 0,1, or 2 substituents independently chosen from halogen, hydroxyl, amino, cyano, -COOH, -CONH₂, CrC4alkyl, Cj-C₄alkoxy, mono- and di-C1־C4alkylamino, trifluoromethyl, and trifluoromethoxy

[0148] (10) The invention includes compounds and salts wherein n is 0, Z is absent, and Y and M are taken together to form a ring, so that

Z

I .

<img file="IL196256A_D0042.tif" />

is a group for the formula:

<img file="IL196256A_D0043.tif" />

Within this embodiment G1, G2, G3, G4, and G5 are independently CH2, O, S, or NR22; wherein no more than two of G!, G2, G3, G4, and G5 are other than CH2.

[0149] R2j is 0 to 2 substituents independently chosen from halogen, hydroxy, C!Cjalkyl, and C1-C₂alkoxy;

[0150] R-22 is independently chosen at each occurrence from hydrogen and methyl; and [0151] R23 is 0 to 2 substituents independently chosen from halogen, hydroxy, C!C2alkyl, and C!-C2alkoxy.

(11) ^,The invention includes compounds and salts in which n is 0, M is hydrogen, Z is absent, and Y and J are taken together to form a ring, so that

Z

<img file="IL196256A_D0044.tif" />

is a group for the formula:

or

<img file="IL196256A_D0045.tif" />

<img file="IL196256A_D0046.tif" />

<img file="IL196256A_D0047.tif" />

<img file="IL196256A_D0048.tif" />

<img file="IL196256A_D0049.tif" />

within this embodiment G_b G2, G3, and G&lt; are independently CH₂,0, S, orNRzf, wherein no more than two of G1, G₂, G3, and G4 are other than CH!.

[0152] Ra! is 0 to 2 substituents independently chosen from halogen, hydroxy, C!C₂alkyl, and C!-C₂alkoxy;

[0153] R22 is 0 to 2 substituents independently chosen from halogen, hydroxy, C!C₂alkyl, and C_I-C₂alkoxy; and

[0154] R23 is 0 to 2 substituents independently chosen from halogen, hydroxy, CiC₂alkyl, and C!-C₂alkoxy.

[0155] (12) The invention further includes compounds and salts of wherein

<img file="IL196256A_D0050.tif" />

is a group for the formula:

<img file="IL196256A_D0051.tif" />

<img file="IL196256A_D0052.tif" />

<img file="IL196256A_D0053.tif" />

[0156] (13) The invention includes compounds and n is 0, Z is absent, and Y and L are taken together to form a ring, so that

Z

<img file="IL196256A_D0054.tif" />

is a group for the formula:

<img file="IL196256A_D0055.tif" />

<img file="IL196256A_D0056.tif" />

[0157] Within this embodiment G_b G₂, G₃, and G₄ are independently CH₂, O, S, orNR22; wherein no more than two of G!, G_2> G3, and G₄ are other than CH₂.

[0158] Qi and Q2 are independently CH orN.

[0159] Rai is 0 to 2 substituents independently chosen from halogen, hydroxy, CiC₂alkyl, and C_rC₂alkoxy;

[0160] R22 is 0 to 2 substituents independently chosen from halogen, hydroxy, CjC₂alkyl, and C!-C2alkoxy; and

[0161] R23 is 0 to 2 substituents independently chosen from halogen, hydroxy, CiC₂alkyl, and C1-C₂alkoxy.

PC17US2007/016018

[0162] (14) The invention includes compounds and salts wherein

Z

<img file="IL196256A_D0057.tif" />

is a group for the formula:

<td> \ / N—&lt; c Kr</td><td> -J׳^NHJ*? ־K n־ 0 0</td><td> 1 1 /~z o־־־־Z^\ ¹¹ \׳״^zηλ/' 1</td><td> 1 n/ν' O^&lt;stf^z’־־^t־k 1 z*^znn/» 1</td><td> ' / N—&lt; c Kr</td>

<td> 0(^0</td><td> HN ήγ!κ</td><td> N—ך nLA. 4+</td><td> 0 0</td><td> Pl Ή o o</td>

<td> -O o o</td><td> ε—\ ^׳ר׳ג \ / N—&lt; &lt; -K n־ o o</td><td> /-0 j^Y \ / N—&lt; c HF</td><td> y—NH t / N—&lt; r H r־ 0 o</td><td> 1—S t /</td>

<td> HN-n -K n־ OO</td><td> -O o o</td><td> -o-</td><td></td><td> -ס-</td>

<td> ־KTr</td><td> N—Γ ς</td><td> ολ ^o;־S5rx J *vv* 1</td><td> 0 0</td><td> -Q</td>

<td> xT-</td><td> kX</td><td> ־kX</td><td> מ ^*ר׳ג \ / N—&lt;e סס?</td><td> o</td>

<td> HN׳־^S</td><td> X</td><td> &lt;°N \ / N—&lt; c K r</td><td> jA^NH KT</td><td> V / N—k c Hr</td>

<td> MN^X_UX$&lt;-</td><td> XT</td><td> jA&lt;^o\ / N-\ c -K H o o</td><td> r ^,*s ^ר׳ג ־o-</td><td> γ^νη ^J'7^^&gt; ־K־r־</td>

<td> r^° \ / N—k c -K H 0 o</td><td> -o-</td><td> -x&gt;</td><td>^s&gt;&lt;^s TT-</td><td> -TT-</td>

<td> X^s\ / N“־k r Ar</td><td> HN-NH \ / N—&lt; r ־Hr־ ο o</td><td> /—NH Λ \ / N—&lt; c Kr_</td><td> /י^׳׳ג Hr</td><td> XT</td>

<td>⁸'''׳'־I</td><td> HN—1 HN 1 -o 0 o</td><td> HN-NH -xl.-</td><td> -ס-</td><td> 4t</td>

<img file="IL196256A_D0058.tif" />

[0163] (15) The invention includes compounds and salts of Formula VH and Formula

VIH in which Y is oxygen.

<img file="IL196256A_D0059.tif" />

<img file="IL196256A_D0060.tif" />

Formula VII

[0164] Within this embodiment:

[0165] R! and R2 are joined to form a pyrrolidine, piperidine, or piperazine ring or a pippra-zinn ring fused to a phenyl, each of which is optionally substituted with 0 to 2 substituents independently chosen from halogen, hydroxyl, amino, CONH2, -COOH, C!-C2alkyl, and C!~ Cialkoxy.

[0166] Rs, R4, R«, and Rs are independently chosen from hydrogen, C!-C₄alkyl, and (C3C7cycloalkyl) C0-C2alkyl.

[0167] R9 is hydroxyl, amino, -COOH, -NR10R11, -OR!2, ־NR!0SO2Rn, -(C=O)OR10, or -CONRjoR״.

[0168] Rjq.R״, and R!2 are independently hydrogen, or C!־C6alkyl, C₂-C6alkenyl, C₂C6alkynyl, (C3-C7cycloalkyl)C0־C2alkyl, (heterocycloalkyl)C₀-C2alkyl, (phenyl)C0־C2alkyl, or (5* to 6-membered monocyclic heteroaryl)C0-C2alkyl, each of which is substituted with 0 to 3 substituents independently chosen from halogen, hydroxyl, oxo, C_t־C2aUcyl, C!-C2alkoxy, trifluoromethyl, and trifluoromethoxy.

[0169] R16 is 0 to 2 substituents independently chosen from halogen, C!-C2alkyl, and C!C2alkoxy.

[0170] M is hydrogen or methyl.

[0171} (11) Z is a quinoline of the formula

<img file="IL196256A_D0061.tif" />

'Ύ'.׳ in which,

Ra! represents a substituent at the 7-position of the quinoline, and 0 to 2 additional substituents independently chosen from halogen, hydroxyl, amino, cyano, -CONHj, -COOH, C1-C₄alkyl, C₂-C₄alkanoyl, C1-C₄alkoxy, mono- and di-Ci-Qjalkylamino, C!C₂haloalkyl, and C!-C₂haloalkoxy; and

[0172] R32 is (phenyl)Co -C₂alkyl or (pyridyl)C0־C₂alkyl, each of which is substituted with 0,1, or 2.substituents independently chosen from halogen, hydroxyl, amino, cyano, COOH, -CONHj, Cj-C4alkyl, C!-C4alkoxy, mono- and di-C1-C₄alkylamino, trifluoromethyl, and trifluoromethoxy.

Pharmaceutical Preparations

[0173] Compounds of the invention can be administered as the neat chemical, but are preferably administered as a pharmaceutical composition. Accordingly, the invention provides pharmaceutical formulations comprising a compound or pharmaceutically acceptable salt of the invention, together with at least one pharmaceutically acceptable earner.

[0174] Compounds of the invention may be administered orally, topically, parenterally, by inhalation or spray, sublingually, transdermally, via buccal administration, rectally, as an ophthalmic solution, or by other means, in dosage unit formulations containing conventional pharmaceutically acceptable carriers. The pharmaceutical composition may be formulated as any pharmaceutically useful form, e.g., as an aerosol, a cream, a gel, a pill, a capsule, a tablet, a syrup, a transdermal patch, or an ophthalmic solution. Some dosage forms, such as tablets and capsules, are subdivided into suitably sized unit doses containing appropriate quantities of the active components, e.g., an effective amount to achieve the desired purpose.

[0175] Carriers include excipients and diluents and must be of sufficiently high purity and sufficiently low toxicity to render them suitable for administration to the patient being treated. The carrier can be inert or it can possess pharmaceutical benefits of its own. The amount of carrier employed in conjunction with the compound is sufficient to provide a practical quantity of material for administration per unit dose of the compound.

[0176] Classes of carriers include, but are not limited to binders, buffering agents, coloring agents, diluents, disintegrants, emulsifiers, flavorants, glidents, lubricants, preservatives, gtahiligers, surfatitant’s, tableting agents, and wetting agents. Some earners may be listed in more than one class, for example vegetable oil may be used as a lubricant in some formulations and a diluent in others. Exemplary pharmaceutically acceptable earners include sugars, starches, celluloses, powdered tragacanth, malt, gelatin; talc, and vegetable oils. Optional active agents may be included in a pharmaceutical composition, which do not substantially interfere with the activity of the compound of the present invention.

[0177] Binders are substances that bind or glue powders together and make them cohesive by forming grannies, thus serving as the adhesive in the formulation. Binders add cohesive strength to that already available in the diluent or bulking agent. Examples of binders include starch, gelatin, natural sugars, com sweeteners, natural and synthetic gums such as acacia, sodium alginate, carboxymethylcellulose, polyethylene glycol and waxes. The amount of binder in the composition can range, for example, from about 2 to about 20% by weight of the composition, or from about 3 to about 10% by weight, even more preferably from about 3 to about 6% by weight.

[0178] Diluents include sugars such as lactose, sucrose, mannitol and sorbitol; starches derived from wheat, com, rice and potato; and celluloses such as !merocrystalline cellulose. The amountofdiluent in the composition may be, for example, about 10 to about 90% by weight of the total composition, about 25 to about 75%, about 30 to about 60% by weight, or about 12 to about 60%.

[0179] Disintegrants are materials added to a pharmaceutical composition to help it break apart (disintegrate) and release the active agent Suitable disintegrants include starches; including cold water soluble modified starches such as sodium carboxymethyl starch; natural and synthetic gams such as locust bean, karaya, guar, and tragacanth gum and agar; cellulose derivatives such as methylcellulose and sodium carboxymethylcellulose; microcrystallme celluloses and cross-linked microcrystalline celluloses such as sodium croscarmellose; alginates such as alginic acid and sodium alginate; clays such as bentonites; and effervescent mixtures.

The amount of disintegrant in the composition can range, for example, from about 2 to about 15% by weight of the composition ar from about 4 to about 10% by weight

[0180] Lubricants are substances added to a pharmaceutical formulation to enable the tablet granules, etc. after it has been compressed, to release from the; mold or die by reducing friction or wear. Examples of lubricants useful in pharmaceutical dosage forms include boric acid, sodium benzoate, sodium acetate, sodium chloride, and the like. Lubricants are usually added at the very last step before tablet compression, since they must be present on the surfaces of the granules and in between them and the parts of the tablet press. The amount of lubricant in the composition can range, for example, from about 0.1 to about 5% by weight of the composition, from about 0.5 to about 2%, or from about 0.3 to about 1.5% by weight. The amount of compound or salt of the invention in a unit dose may be generally varied or adjusted from about 1.0 milligram to about 1,000 milligrams, from about 1.0 to about 900 milligrams, from about 1.0 to about 500 milligrams, or from about 1 to about 250 milligrams, according to the particular application and the potency of the compound. The actual dosage employed may be varied depending upon the patient's age, sex, weight and severity of the condition being treated.

[0181] Pharmaceutical compositions formulated for oral administration are often preferred. These compositions contain between 0.1 and 99% of a compound of the invention and usually at least about 5% (weight %) of a compound of the invention. Some embodiments contain from about 25% to about 50% or from 5% to 75 % of a compound of invention.

Liquids formulations

[0182] Compounds of the invention can be incorporated into oral liquid preparations such as aqueous or oily suspensions, solutions, emulsions, syrups, tinctures, syrups, or elixirs, for example. Moreover, formulations containing these compounds can be presented as a dry product, e.g. as granules or powders, for constitution with water or other suitable vehicle before use. Typical components of carriers for syrups, elixirs, emulsions and suspensions include ethanol, glycerol, propylene glycol, polyethylene glycol, liquid sucrose, sorbitol and water. Liquid preparations can contain conventional additives, such as suspending agents (e.g., sorbitol syrup, methyl cellulose, glucose/sugar, syrup, gelatin, hydroxyethyl cellulose, carboxymethyl cellulose, aluminum stearate gel, and hydrogenated edible fats), emulsifying agents (e.g., lecithin, sorbitan monsoleate, or acacia), non-aqueous vehicles, which can include edible oils (e.g., almond oil, fractionated coconut oil, silyl esters, propylene glycol and ethyl alcohol), and preservatives (e.g., methyl or propyl p-hydroxybenzoate and sorbic acid). . Oral formulations may contain demulcent, flavoring agents, sweetening agents, such as sucrose or saccharin, tastemasking agents, and coloring agents.

Suspensions

[0183] Aqueous suspensions contain the active material(s) in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example AVICEL RC-591, sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents, for example lecithin and polysorbate 80. The aqueous suspensions may also contain one or more preservatives, for example ethyl, n- propyl phydroxybenzoate, methyl parabens, propyl parabens, and sodium benzoate.

[0184] Oily suspensions may be formulated by suspending the active ingredients in a vegetable oil, for example peanut oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.

Emulsions

[0185] Pharmaceutical compositions of the invention may also be in the form of oil-inwater emulsions- The oily phase may be a vegetable oil, for example olive oil or peanut oil, or a mineral oil, for example liquid paraffin or mixtures of these. Suitable emulsifying agents may be naturally-occurring gums, for example gum. acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol, anhydrides, for example sorbitan monoleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monoleate.

Tablets and Capsules

[0186] Tablets typically comprise conventional pharmaceutically compatible adjuvants as inert diluents, such as calcium carbonate, sodium carbonate, mannitol, lactose and cellulose; binders such as starch, gelatin and sucrose; disintegrants such as starch, alginic acid and croscarmelose; lubricants such as magnesium stearate, stearic acid and talc. Glidants such as silicon dioxide can be used to improve flow characteristics of the powder mixture. Coloring agents, such as the FD&amp;C dyes, can be added for appearance. Sweeteners and flavoring agents, such as aspartame, saccharin, menthol, peppermint, and fruit flavors, are useful adjuvants for chewable tablets. Capsules (including time release and sustained release formulations) typically comprise one or more solid diluents disclosed above. The selection of earner components often depends an secondary considerations like taste, cost, and shelf stability.

[0187] Such compositions may also be coated by conventional methods, typically with pH or time-dependent coatings, such that the subject compound is released in the gastrointestinal tract in the vicinity of die desired topical application, or at various times to extend the desired action. Such dosage forms typically include, but are not limited to, one or more of cellulose acetate phthalate, polyvinylacetate phthalate, hydroxypropyl methylcellulose phthalate, ethyl cellulose, Eudragit coatings, waxes and shellac.

[0188] Formulations for oral use may also be presented as hard or soft shell capsules. A capsule is a dosage form provided in a special container or enclosure containing an active agent. The active agent may be present in solid, liquid, gel, or powder form, or any other pharmaceutically acceptable form. A capsule shell may be made of methyl cellulose, polyvinyl alcohols, or denatured gelatins or starch or other material. Hard shell capsules are typically made of blends of relatively high gel strength bone and pork skill gelatins. Soft shell capsule shells are often made of animal or plant gelatins. The capsule itself may contain small amounts of dyes, opaquing agents, plasticizers and preservatives.

[0189] The active agent in a capsule may be mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or in the case of soft gelatin capsules the active ingredient may be mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.

Injectable and Parenteral formulations

[0190] Pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleaginous suspension- This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents that have been mentioned above. The sterile injectable preparation may also be sterile injectable solution or suspension in a non-toxic parentally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer’s solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are usefill in the preparation of injectables.

[0191] Compounds of the invention may be provided parenterally in a sterile medium. Parenteral administration includes subcutaneous injections, intravenous, intramuscular, intrathfrad injection or infusion techniques. The drug, depending on the vehicle and concentration used, can either be suspended or dissolved in the vehicle. Advantageously, adjuvants such as local anesthetics, preservatives and buffering agents can be dissolved in the vehicle. Tn compositions for parenteral administration the carrier typically comprises least about 90% by weight of the total composition.

Packaged Formulations

[0192] The invention includes packaged pharmaceutical combinations. Such packaged combinations include a compound of Formula I in a container. The container may additionally include instructions for using the combination to treat or prevent a viral infection, such as a hepatitis C infection, in a patient.

[0193] The packaged pharmaceutical combination may include one or more additional active agents.

Methods of Treatment

[0194] The invention includes methods of preventing and treating hepatitis C infections, by providing an effective amount of a compound of the invention to patient at risk for hepatitis C infection or infected with a hepatitis C virus.

[0195] The pharmaceutical combinations disclosed herein are useful for preventing and treating hepatitis C infections in patients. An effective amount of a pharmaceutical combination of the invention may be an amount sufficient to (a) prevent hepatitis C or a symptom of a hepatitis C from occurring in a patient who may be predisposed to hepatitis C but has not yet been diagnosed as having it or prevent diseases that may be associated with or caused by a primary hepatitis C infection (such as liver fibrosis that can result in the context of chronic HCV infection); (b) inhibit the progression of hepatitis C; and (c) cause a regression of the hepatitis C infection. An amount of a pharmaceutical composition effect to inhibit the progress or cause a regression of hepatitis C includes an amount effective to stop the worsening of symptoms of hepatitis C or reduce the symptoms experienced by a patient infected with the hepatitis C virus.

Alternatively a halt in progression or regression of hepatitis C may be indicated by any of several markers for the disease. For example, a lack of increase or reduction in the hepatitis C viral load or a lack of increase or reduction in the number of circulating HCV antibodies in a patient s blood are markers of a halt in progression or regression of hepatitis C infection. Other hepatitis C disease markers include aminotransferase levels, particularly levels of the liver enzymes AST and ALT. Normal levels of AST are from 5 to 40 units per liter of serum (the liquid part of the blood) and normal levels of ALT are from 7 to 56 units per liter of serum. These levels will typically be elevated in a HCV infected patient Disease regression is usually marked by the return of AST and ALT levels to the normal range.

[0196] Symptoms of hepatitis C that may be affected by an effective amount of a pharmaceutical combination of the invention include decreased liver function, fatigue, flu-like symptoms: fever, chills, muscle aches, joint pain, and headaches, nausea, aversion to certain foods, unexplained weight loss, psychological disorders including depression, tenderness in the abdomen, and jaundice.

[0197] “Liver function” refers to a normal function of the liver, including, but not limited to, a synthetic function including synthesis of proteins such as serum proteins (e.g., albumin, slotting factors, alkaline phosphatase, aminotransferases (e.g., alanine transaminase, aspartate transaminase), 5'־nucleosidase, y glutaminyltranspeptidase, etc.), synthesis of bilirubin, synthesis of cholesterol, and synthesis of bile acids; a liver metabolic function, including carbohydrate metabolism, amino acid and ammonia metabolism, hormone metabolism, and lipid metabolism; detoxification of exogenous drugs; and a hemodynamic function, including splanchnic and portal hemodynamics.

[0198] An effective amount of a combination described herein will also provide a sufficient concentration of the active agents in the concentration when administered to a patient. A sufficient concentration of an active agent is a concentration of the agent in the patient's body necessary to prevent or combat the infection. Such an amount may be ascertained experimentally, for example by assaying blood concentration of the agent, or .theoretically, by calculating bioavailability. The amount of an active agent sufficient to inhibit viral infection in vitro may be determined with a conventional assay for viral infectivity such as a replicon based assay, which has been described in the literature.

[0199] The invention also includes using pharmaceutical combinations comprising a compound of the invention and at least one additional active agent in prophylactic therapies. In the context of prophylactic or preventative treatment an effective amount of a compound of the invention is an amount sufficient to significantly decrease the patient’s risk of contracting a hepatitis C infection.

[0200] Methods of treatment include providing certain dosage amounts of a compound of the invention ahd the at least one additional active agent to a patient. Dosage levels of each active agent of from about 0.1 mg to about 140 mg per kilogram of body weight per day are useful in the treatment of the above-indicated conditions (about 0.5 mg to about 7 g per patient per day). The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the patient treated and the particular mode of administration. Dosage unit forms will generally contain between from about 1 mg to about 500 mg of each active agent. In certain embodiments 25 mg to 500 mg, or 25 mg to 200 mg of a compound of the invention are provided daily to a patient. When the additional active agent is NM 283 (valopicitabine), 100 mg to 1000 mg/ day, or 200 mg to 800 mg/day, or 200 to . 400 mg/ day of either of those agents are typically provided to the patient. When the additional active agent is VX-950,1000 mg to 3750 mg/ day, or 1200 mg to 1800 mg/day are administered to the patient. Treatment regiments in which VX-950 is an additional active agent and about 350 to about 450 mg or about 700 to about 800 mg of VX-950 are administered to a patient three ti-mas per day or about 350 to about 450 mg or about 700 to about 800 mg is administered every 12 hours are particularly included in the invention.

[0201] Frequency of dosage may also vary depending on the compound used and the particular disease treated. However, for treatment of most infectious disorders, a dosage regimen of 4 times daily or less is preferred and a dosage regimen of 1 or 2 times daily is particularly prefened.

[0202] It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drag combination and die severity of the particular disease undergoing therapy.

־ 48

Combination Methods

[0203] The invention includes methods of treatment in which a compound or salt of the invention is provided together with one or more additional active agents. In certain embodiments the active agent (or agents) is an HCV protease inhibitor or HCV polymerase inhibitor. Far example the protease inhibitor may be telaprevir (VX-950) and the polymerase inhibitor may be valcpicitabine, or NM 107, the active agent which valopicitabine is converted into in vivo. In certain embodiments the second active agent is ribavirin, interferon, or Peginterferon alpha conjugate

[0204] According to the methods of the invention, the compound of the invention and an additional active agent may be: (1) co-fonnulated and administered or delivered simultaneously in a combined formulation; (2) delivered by alternation or in parallel as separate formulations; or (3) by any other combination therapy regimen known in the art When delivered in alternation therapy, the methods of the invention may comprise administering or delivering the compound of The invention and an additional active agent sequentially, e.g., in separate solution, emulsion, suspension, tablets, pills or capsules, or by different injections in separate syringes. In general, during alternation therapy, an effective dosage of each active ingredient is administered sequentially, i.e., serially, whereas in simultaneous therapy, effective dosages of two or more active ingredients are administered together. Various sequences of intermittent combination therapy may also be used.

[0205] In certain embodiments method of treatment includes providing a patient with a compound of Formula I and an interferon such as a pegylated interferon or interferon gamma. The interferon may be the only compound provided with the compound of the invention or may be provided with an additional active agent that is not an interferon.

[0206] The invention methods of treatment and pharmaceutical combinations including compounds of the invention any one or combination of the following compounds and substances as an additional active agent:

Caspase inhibitors: EDN 6556 (Idun Pharmaceuticals)

Cvclophilin Inhibitors: NIM811 (Novartis) andDEBIO-025 (Debiqphann)

Cytochrome P450 monooxygenase inhibitors: ritonavir (WO 94/14436), ketoconazole, troleandomycin, 4-methyl pyrazole, cyclosporin, cl om ethiazole, cimetidine, itraconazole, fluconazole, miconazole, fluvoxamine, fluoxetine, nefezodone, sertraline, indinavir, nelfinavir, amprenavir, fosamprenavir, saquinavir, lopinavir, delavirdine, erythromycin, VX-944, and VX497. Preferred CYP inhibitors include ritonavir, ketoconazole, troleandomycin, 4-methyl pyrazoTe, cyclosporin, and clomethiazole

Glucocorticoids: hydrocortisone, cortisone, prednisone, prednisolone, methylprednisolone, triamcinolone, paramethasone, betamethasone, and dexamethasone

Hematopoietins: hematopoietin-1 and hematopoietin-2. Other members of the hematopoietin superfamily such as the various colony stimulating factors (e.g. (e.g. G-CSF, GMCSF, M-CSF), Epo, and SCF (stem cell factor)

Homeopathic Therapies: Milk Thistle, silymarin, ginseng, glycyrrhizm, liconceroot, schisandra, vitamin C, vitamin E, beta carotene, and selenium trnmunnmodulatorv compounds: thalidomide, IL-2, hematopoietins, IMPDH inhibitors, for example Merimepodib (Vertex Pharmaceuticals Inc.), interferon, including natural interferon (such as OMNIFERON, Viragen and SUMIFERON, Sumitomo, a blend of natural interferons), natural interferon alpha (ALFERON, Hemisphere Biopharma, Inc.), interferon alpha nl from lymphblastoid cells (WELLFERON, Glaxo Wellcome), oral alpha interferon, Peg-interferon, Peg-interferon alia 2a (PEGASYS, Roche), recombinant interferon alfa 2a (ROFERON, Roche), inhaled interferon alpha 2b (AERX, Aradigm), Peg-interferon alpha 2b (ALBUFERON, Human Genome Sciences/Novartis, PEGINTRON, Schering), recombinant interferon alfa 2b (INTRON A, Schering), pegylated interferon alfa 2b (PEG-INTRON, Schering, VIRAFERONPEG, Scaring) ,interferon beta-la (REBIF, Serono, Inc. and Pfizer), consensus interferon alpha (ENFERGEN, Valeant Pharmaceutical), interferon gamma-lb (ACTIMMUNE, Intermune, Inc.), un-pegylated interferon alpha, alpha interferon, and its analogs, and synthetic thymosin alpha 1 (ZADAXIN, SciClone Pharmaceuticals Inc.)

Immunosuores sants: sirolimus (RAPAMUNE, Wyeth)

Interleukins: (IL-1, IL-3, IL-4, IL-5, IL-6, IL-10, IL-11, IL-12), LIF, TGF-beta, TNFalpha) and other low molecular weight factors (e.g. AcSDKP, pEEDCK, thymic hormones, and minicytokines)

Interferon Enhancers: EMZ702 (Transition Therapeutics)

TRES inhibitors: VGX-410C (VGX Pharma)

Monoclonal and Polyclonal antibodies: XTL-6865 (XTL), HuMax-HepC (Genmab), Hepatitis C Immune Globin (human) (CIVACIR, Nabi Biophannceuticals)

Nucleoside analogues: Lamivudine (EPTVIR, 3TC, GlaxoSmithKline), MK-0608 (Merck), zalcitahine (HTVID, Roche US Pharmaceuticals), ribavirin (including COPEGUS (Roche), REBETOL (Schering), VILONA (ICN Pharmaceuticals, and VIRAZOLE (ICN Pharmaceuticals), and viramidme (Valeant Pharmaceuticals), an amidine prodrug of ribavirin. Combinations .of nucleoside analogues may also be employed.

Non-nucleoside inhibitors: PSI-6130 (Roche/ Phannasset), delaviridine (RESCRIPTOR, Pfizer), and HCV-796 (Virophann)

P7 protein inhibitor: amantadine (SYMMETREL, Endo Pharmaceuticals, Inc.) Polymerase inhibitors: NM283 (valqpicitabine) (Idenix) andNM 107 (Idenix).

Protease inhibitors: BILN-2061 (Boehringer Ingelheim), GW-433908 (prodrug of Amprenavir, Glaxo/ Vertex), indinavir (CRIXIVAN, Merck), ΓΓΜΝ-191 (Intermune/ Array Biophanna), VX950 (Vertex) and combinations comprising one or more of the foregoing protease inhibitors

RNA interference: SIRNA-034 RNAi (Sima Therapeutics)

Therapeutic Vaccines: IC41 (Intercell), IMN-0101 (Imnogenetics), GI5005 (Globeimmune), Chronvac-C (Tripcp/Inovio), ED-002 (Imnogenetics), Hepavaxx C (ViRex Medical)

TNF agonists: adalimumab (HUMIRA, Abbott), entanercept (ENBREL, Amgen and Wyeth), infliximab (REMICADE, Centocor, Inc.)

Tubulin inhibitors: Colchicine

Snhingosine-1-phosphate receptor modulators: FTY720 (Novartis)

TLR agonists: ANA-975 (Anadys Pharmaceuticals), TLR7 agonist (Anadys Pharmaceuticals), CPG10101 (Coley), andTLR9 agonists including CPG 7909 (Coley)

Cvclophilin Inhibitors: NIM811 (Novartis) and DEBIO-025 (Debiqpharm)

[0207] Patients receiving hepatitis C medications are typically given interferon together with another active agent. Thus methods of treatment and pharmaceutical combinations in which a compound of The invention is provided together with an interferon, such as pegylated interferon alfa 2a, as the additional active agents are included as embodiments. Similarly methods and pharmaceutical combinations in which ribavirin is an additional active agent are provided herein.

PCT/US2007/0160I8

EXAMPLES

Abbreviations

[0208] The following chemical abbreviations are used in Example 1. Additional abbreviations used in these examples will be familiar to those of skill in the art of organic chemical synthesis.

<td> CDI</td><td> 1,1 ^,-Carbonyldiimidazole</td>

<td> DBU</td><td> Diazabicyclo[5.4.0]undec-7-ene</td>

<td> DCM DIEA DMF</td><td> Dichloromethane N, N-Diisopropylethyl amine Dimethyl formamide</td>

<td> HATU HBTU NMM</td><td> O-(7-azabenotriazol-l~yl)-l, 1,3,3-tetramethyluronium O-(12T-Benzotriazol-l-yI) N, Ν,Ν’,Ν -tetramethyluronium hexafluorophosphate N-methylmorpholine</td>

<td> RCM</td><td> Ring-closing metathesis</td>

<td> TEA TFA</td><td> Triethylacetate Trifluoroacetic acid</td>

EXAMPLE 1. SYNTHESIS OF 1-((2S,4R)-1-((S>2-TERT־BUTYL-4-OXO-4-(PIPER1DIN-1yl)butanoyl)-4-(7-methoxy-2-phenylquinolin-4־yloxy)pyrroudine-2-carboxam1do)-2VINYLCYCLOPROPANECARBOXYL1C ACID

Step 1. Preparation of N-(cyclopropylsulfonyl)-l-(BOC-amino)*2vinylcyclopropanecarboxamide

<img file="IL196256A_D0062.tif" />

[0209] CDI (2.98 g, 18.4 mm, 1.1 eq) is dissolved in ethyl acetate. N-Boccyclopropylvinyl acid (3.8 g, 16.7 mm, 1.0 eq), prepared via the procedure given by Beaulieu,

P.L. et al. (J. Org. Chem. 70; 5869-79 (2005)) is added to the CDI/ ethyl acetate mixture and stirred at RT until the starting material is consumed. Cyclopropyl sulfonamine (2.2 g, 18.4 mm,

1.1 eq) is added to this mixture followed by DBU (2.1 ml, 20.5 mm, 1 23 eq) and the mixture is stirred at RT for 2 h. Workup and purification by silica gel chromatography provides 2g of compound 2.

Step 2. Preparation of (2S,4R)-tert-butyl 2-(l-(cyclopropylsulfonylcarbamoyl)-2vinylcyclopropylcarbamoyl)-4-(7-methoxy-2-phenylquinolin-4-yloxy)pyrrotidine-l-carboxylate and(2S,4R)*N-(l-(cyclopropylsulfonylca1bamoyi)-2-vinylcyclopropyl)-4-(7-methoxy2־pheriylquinolin-4-yloxy)pyrTolidine-2-carboxan1ide

<img file="IL196256A_D0063.tif" />

<img file="IL196256A_D0064.tif" />

[0210] Compound 1 (4.3g, 9.3mmol, 1-1 eq), prepared according to the method given 1ns WO 02/060926, in DMF is stirred with O-(Benzotriazol-lyl)-N,N,N’,N’-Tetramethyluromum hexafluorophosphate (4.1g,10.5mmol, 1.3eq) for 30 minutes, followed by addition of cyclopropylamine 2 (1.92g, 8.3mmol, 1.0eq)andN-mcthylmorpholine (2.52g, 25.0mmol,3.0eq). The mixture is stirred over night and the solvent removed under reduced pressure. The resulting residue is diluted with ethyl acetate and washed with saturated aqueous NaHCO₃. The organic solvent is dried over MgSO₄ and concentrated under reduced pressure to afford crude 3, which is used for next step without further purification.

[0211] Compound 3 in 10 ml dry CH2C12 is treated with 5 mL TFA and stiired over night. The solvent is removed and the residue recrystallized from ethyl acetate to afford 4.12 g Compound 4 (61% yield two steps).

PCT7US2007/016018

Step 3. Preparation of (3S)-3-((2S,4R)-2-(l~(cycIopropylsulfonylcarbamoyI)-2vmylcyclopropylcarbamoyl)-4־7)־methoxy-2-phenylquinolin-4-yloxy)pyrrolidine-l-carbonyl)-

4,4-dimethylpentanoic acid

<img file="IL196256A_D0065.tif" />

[0212] The Acid 5 (58mg, 0.25mmol, 1.2eq), prepared via the procedure given by Evans, D.A., et al. (J. Org. Chem. 64: 6411- 6417 (1999)) in 1.2 mL DMF is stirred with 4 (138mg, 0/21 mmol), HATU (160mg, 0.42mmol, 2.0eq), and DEEA (0.63mmol, 3.0 eq) overnight The mixture is subjected to HPLC purification to afford 121 mg 6 (77% yield), which is further treated with 0.5 mL TFA in 1.0 mL DCM overnight. The solvent was removed to provide Compound 7 in 100% yield.

Step 4. Preparation of (2S,4R)-l-((S)־2־tert־butyl-4-oxo-4-(piperidin-l-yl)butanoyl)-N-(l(cyclopropylsulfonylcarbamoyl)-2-vinylcyciopropyl)-4-(7-methoxy-2-phenylquinolm*4yloxy)pyrrolidine-2-carboxamide

<img file="IL196256A_D0066.tif" />

[0213] The Acid 7 (0.15mmol) in 1.0 mL DMF is stirred with pepridine (excess, 0.6mmol, 4eq), HATU (115mg, 0.3mmol, 2.0eq), and DIEA (0.45mmol, 3.0eq) for 4 hrs. The mixture is subjected to HPLC purification to afford 77.1mg 8.

Step 5. Preparation of (3S)-3-((2S,4R)-2-(l-(ethoxycafbonyl)*2-vinylcyclopropylcarbamoyl)-4(7-methcxy-2-phenylquinolin-4-yloxy)pyrrolidine-1 -carbonyl)-4,4-dimethylpentanoic acid

<img file="IL196256A_D0067.tif" />

Step 5. Preparation of (3S)-3-((2S,4R)-2-(l-(ethoxycarbonyl)-2-vinylcyclopropylcarbamoyl)-4(7-methoxy-2-phenylquinolin-4-yloxy)py1rolidine-1 -carbonyl)-4,4-dimethylpentanoic acid

[0214] The Acid 5 (105mg, 0.46mmol, 1.2eq) in 12 mL DMF is stirred with 9 (202mg, O.38rmnol), HATU (290mg, 0.76mmol, 2.0eq), and DIEA (1.2mmol, 3.0eq) overnight The mixture is subjected to HPLC purification to afford 204.3mg 10 (75% yield), which is further treated with 0.5 mL TFA in 1.0 mL DCM overnight The solvent is removed to provide 11 in

<img file="IL196256A_D0068.tif" />

<img file="IL196256A_D0069.tif" />

Step 6. Preparation of final product

[0215] The Acid 11 (30mg, 0.045mmol) in 1.0 mL DMF is stirred with pepridine (0.27mmol, 6eq), HATU (34mg, 0.09mmol, 2.0eq), and DIEA (0.14mmol, 3.0eq) for 2 hrs. The mixture is subjected to HPLC purification to afford 21.2 mg 12 (65% yield), which is hydrolyzed in methanol with 2N NaOH for 6 hrs. The mixture is acidified with 6N HC1 and subjected to HPLC purification to afford 7.6 mg 13.

Example 2. Synthesis of Macrocyclic Compounds

General Procedures

Procedure 1. DeProtection Reaction (Conversion of N-Boc to amine or t-butyl ester to carboxylic acid)

[0216] TFA (3-4ml) is added to a solution of Boc protected starting material (lmmol) or t-butyl ester in anhydrous DCM (7ml) at room temperature. ^,The reaction is monitored with LC/MS and TLC. After 1-3 hrs, the reaction mixture is evaporated reduced pressure to dryness. The crude product is used for next step reaction without further purification.

Procedure 2. Amide formation

[0217] N-methylmoipholine (2mmol) and HBTU (l.Zmmol) are added in one portion at room temperature to a solution of acid (lmmol) in anhydrous DMF (10ml). After stirring at room temperature 10 minutes, amine (1 mmol) is added in one portion and then stirred overnight. The reaction mixture is poured into ice-water and extracted with ethyl acetate (100ml). The organic layer is washed with H2O, brine, and dried over anhydrous MgSO4- The residue is filtered and evaporated in vacuum to dryness. The crude product is purified by flash chromatography on silica gel (hexane-ethyl acetate 100:0 to 50:50) to give the desired product

Procedure 3. RCM catalyzed by Grubbs 2^nd (CAS Reg. No. 246047-72-3) or HoveydaGrubbs 2^nd catalyst (CAS Reg. No. 301224-40-8)

[0218] A mixture of starting material (di-olefin, lmmol), catalyst (5~30mol%) in 1,2dichloroethane is degassed and heated to 110 °C for 12—24hrs under atmosphere of argon. The reaction is monitored by LC/MS and TLC. ”The reaction mixture is evaporated to dryness under reduced pressure. The crude product is purified by flash chromatography on silica gel (hexaneethyl acetate 100:0 to 50:50) to give the desired product.

Procedure 4. Hydrolysis of esters

[0219] LiOH hydrate (6 equiv.) is added in one portion at room temperature to the solution of ester (lmmol) in THF (5ml), methanol (2.5ml), and water (2.5ml), and then the reaction mixture is stirred overnight. After the reaction is complete (by LC/MS), it is cooled to 0°C and acidified to pH~2 and extracted with DCM (20ml x 2). The reaction is dried over MgSO4, filtered, and evaporated to dryness under reduced pressure. The crude product is purified by flash chromatography on silica gel (hexane-ethyl acetate 100:0 to 20:80) to give the desired product

<img file="IL196256A_D0070.tif" />

<img file="IL196256A_D0071.tif" />

<img file="IL196256A_D0072.tif" />

Compound 21 is prepared by procedures 1 and 2 from starting material 14 and amino acid 20.

MS (Nf+1) 754.

Compound 22 is prepared by procedure step 3. MS (Μ*+1) 726.

Compound 23 is prepared by procedures 1 and 2. MS (M^+l) 737.

Compound 24 is prepared by procedure 4. MS (Μ*+1) 709.

Scheme 2. Synthesis of Compounds 34 and 35

<img file="IL196256A_D0073.tif" />

Compound 29 is prepared by procedure 4 from starting material 28.

Compound 30 is prepared by procedure set forth in step 2 with starting material 29.

Compound 31 is prepared by procedure set forth in steps 1 and 2 with starting material 30.

Compound 33 is prepared by procedure set forth in step 4 with starting material 28 and 32.

Compounds 34 and 35 are prepared by toe procedure set forth in step 3. After RCM, toe reaction mixture is separated by prep-TLC (hexane -ethyl acetate 1:1) to afford compounds 34 and 35.

MS(M*812 = (1+־

Preparation of intermediates 20 and 32

<img file="IL196256A_D0074.tif" />

Step 4

<img file="IL196256A_D0075.tif" />

<img file="IL196256A_D0076.tif" />

<img file="IL196256A_D0077.tif" />

Step 1. 8-nonenoic acid (1.56g, lOmmol) is placed in a 100ml flask, anhydrous ether (35ml) is added under N2, cooled to 0°C, TEA (1.6g, 16mxnol) is added, followed by pivaloyl chloride (1.26g, 10.5mmol) dropwise. The ice-bath is removed and the reaction mixture is stirred at room temperature for Ihr. The resulting suspension is cooled to 0°C and filtered into a 250 ml flask under N; (washed twice with anhydrous ether 10ml x 2). The filtrate is cooled to -78°C and diluted with anhydrous THF (25ml).

[0220] A few crystals of 1,10-phenathrolme are added o a solution of (S)(-)-4-benzyl-2oxazolidinone in THF (25ml). The solution is cooled to -78°C and a solution of n-BuLi (1.6M in hexane, 6.5ml, 10.4mmol) is added dropwise until the red color persists for 10 min. This solution is added to the above cooled mixed anhydride solution at -78°C over 20 min via cannula. The resulting mixture is stirred at -78°C for additional 30min, then poured into sat NH4CI, the organic layer is separated, the aqueous layer is extracted with ether (50 ml, 3X). The combined organic layers are washed with brine and dried over MgSO4, filtered, concentrated in reduced pressure to dryness. The crude product is purified by flash chromatography (hexane-ethyl acetate 100:0-100:20) to give 3.01g of26 (95%).

Step 2. A solution of 26 (3.01g, 9.6rnmol) in anhydrous THF is cooled to -78°C, then 2.0m solution of NaN(TMS)2 in hexane (5.76ml) was added dropwise over lOmin. After 30min, t-Butyl bromoacetate was added dropwise at -78°C. The reaction mixture was stirred at-78 °C for 2 hrs. LC/MS and TLC monitored the reaction. The reaction was quenched with 10% KHSO4 to pH—4—6, extracted with ethyl acetate, organic layer was washed with H2O, brine, dried over MgSO4, filtered, evaporated in reduced pressure to dryness. The crude product was purified by chromatography on silica gel (hexane—ethyl acetate 100:0—100:20) to give compound 14.

Step 3. H2O2 (50%, 0.9 ml) is added dropwise over 5 min. at 0 °C, followed by a solution of LiOH (0.2g in 2ml of HjO). The reaction mixture is added to a solution of 27 (1.05g, 2.44 mmol) in ΤΉΕΉ2Ο (5:1,24 ml). The mixture is stirred at 0 °C for Ih and then quenched by dropwise addition .of an aqueous solution of sodium thiosulphate (10 ml) while keeping the temperature below 20°C. The mixture is extracted with ethyl acetate (discarded) and the aqueous phase was acidified to pH—2 with solid citric acid and extracted with ethyl acetate. The combined organic phase is washed with brine, dried over anhydrous sodium sulphate and the solvent t removed under reduced pressure. The residue is purified by column chromatography on silica gel, eluting with a gradient system of hexane: ethyl acetate (100:0) to 50:50 to afford the desired compound 20 (503 mg).

Step 4. Compound 36 is prepared by procedure 1.

Step 5. Compound 37 is prepared by procedure 2.

Step 6. Compound 32 is prepared from 37 by the procedure given in step 3 for the preparation of compound 20.

Example 3. Additional tertiary Amine substituted peptides

<td> No.</td><td> STRUCTURE</td><td> Name</td><td> EC5&lt;</td>

<td> 38</td><td> Q 0 / 4־0 ² 4—0. / \ χ f /־'Τ ² J t \־' /Γ o 'f \ /( ° °χ^ΖΙ °^/ /-ג-- ס 1</td><td> 1 -((2S,4R)-1 -((S)-2-tert-butyl-4-oxo-4(pyrrolidin-1 -yl)butanoyl)-4-(7-methoxy* 2־phenylquinolin-4־yloxy)py1Tolidine-2carboxamido)-2- . vinylcyclopropanecatboxylic acid</td><td> *</td>

<td> 39</td><td> C ΪΓ \ P z y-o / \ / / ° Ο ό</td><td> ethyl 1 -((28,4R)-1 -((S)-2-tert־butyl-4-oxo4-(pyrrolidin-1 -yl)butanoyl)-4-(7methoxy-2-phenylquinolin-4yloxy)pyrrolidi11e-2־carboxan1ido)*2vinylcycloprppanecarboxylate</td><td> * 1</td>

<td> 40</td><td> / Ο \ \ /=° o z A— σ / \ ס=ץ / •</td><td> ethyl 1 -((2S.4R)-1 -((S)-2-tert-butyl-4-oxo4־(piperidin-1 -yl)butanoyl)7)-4־ -methoxy2־phenylquinolm-4-yloxy)pyrrolidine-2carboxamido)-2vinylcyclopropan ecarboxylate</td><td> *</td>

<td> No.</td><td> STRUCTURE</td><td> Name</td><td> EC5</td>

<td> 41</td><td> X o o' \_ *=o /\ -־־^Z w Γ)</td><td> 1 - ((2S,4R)-1 -((S)-2-tert-butyl-4-oxo-4(piperidin-l -yl)butanoyl)7)־4־-mcthoxy-2phenylquinolin-4-yloxy)pyrrolidn1e-2cafboxamido)-2vinylcyclopropanecarboxylic acid</td><td></td>

<td> 42</td><td> X o o=/ Z⁵®' o' ¹²V b ,ch⁰/ o X</td><td> l-((2S,4R)-l-((2S)-2-tert-butyl3)-4־hydroxypyrrolidin-1 -yl)4־-oxobutanoyl)-4(7-methoxy-2־phenylquinolin-4yloxy)pyrrolidine-2-carboxamido)-2vmylcyclopropanecarboxylic acid</td><td></td>

<td> 43</td><td> X o Q־s/ / ^XZλ__ Ϊ=־Ο ΖΆ . y &lt;&gt; / \=o o 0 /</td><td> l-((2S,4R)-l-((S)-2-tert-butyl-4-(4methylpiperazin1 ־ -yl)4־-oxobutanoyl)4־(7-methoxy-2-phenylquinolin-4yloxy)pynrolidine-2-carboxamido)2־vmylcyclopropanecarboxylic acid</td><td></td>

<td> No.</td><td> STRUCTURE</td><td> Name</td><td> EC5(</td>

<td> 44</td><td> I p Q Λ O V—/ /—2 Ο \ / 'ζ £</td><td> l-((2S,4R)-l-((2S)-2-tert-butyl-4-(2carbamoylpiperidin-1 -yl)-4OX0butaD0yl)־ 4-(7־meti1oxy2־-phenylquinoli11-4yloxy)pyrrolidine-2־carboxamido)-2vinylcyclopropanecarboxylic acid</td><td></td>

<td> 45</td><td> 1 9 Λ&gt; ^¹¹״2 ο \--.'Ζ £</td><td> ethyl 1-((2S,4R)2))- 1־S)-2-tert-butyl-4-(2c»rhamoylpiperidin-l-yl)-4-oxobutanoyl)4-(7-methoxy2־-phenylquinolin-4yloxy)pyrroli&lt;ime-2-ca1boxamido)-2vinylcyclopropanecarboxylate</td><td> **</td>

<td> 46</td><td> X ο ־'89 0^09^</td><td> l-((2S,4R)-l-((S)-4-(4-((tertbutoxycarbonylamino)methyl)piperidm-1 yl)-2-tert-butyl-4-oxobutanoyl)-4-(7methoxy2־-phenylquinolin4־yloxy)pyrrolidine-2-carboxamido)-2vinylcyclopropanecarboxylic acid</td><td> *</td>

<td> 47</td><td> 9 ΛΒ=־\ V-Z 1 / \ ע χ \ W ρ 1 \ 1 ^ζ \ J— ΖγΓ⁷־ Ρ ο</td><td> (2R,6S, 16aS,Z)־ethyl 2-(7-mcthoxy2־phenylquinolin-4־yloxy)-5,16-dioxo-6-(2oxo-2-(piperidin-l־yl)ethyl)- 1,2,3,5,6,7,8,9,10,11,13a&gt;14,143,15,16,16ahexadecahydrocyclopropa[e]pyrrolo[l ,2a][ 1,4]diazacyclopentadecine' 14acarboxylate</td><td></td>

<td> No.</td><td> STRUCTURE</td><td> Name</td><td> EC51</td>

<td> 48</td><td> - X O o S3 ο Λ ° r O א</td><td> (2R.6S, 16aS,Z&gt;2-(7-methoxy-2pbenylquinolin-4-yloxy)5,16־-dioxo2)-6־oxo-2-(piperidin־l-yl)ethyl)־ 1,2,3,5,6,7,8,9,10,11,13a,14,14a,15,16,16ahexadecahydrocyclopropa[e]pyrrolo[l ,2a][l ,4]diaz^acyclopentade^cme-l 4acarboxylic acid</td><td></td>

<td> 49</td><td> ¾. f~~\ 0 o^⁰ 'X7 Γ] ( HN—jf μ V ΧζΝγ''—/⁵O U\ יל/ ס s</td><td> [0221] (2S,4R)-1-((2)-2-tert-butyl4-oxo-4־(piperidin-l-yl)butanoyl)-N-(l־ (cyclopropylsulfonylcarbamoyl)-2vmylcyclopTopyl)7)-4־-methoxy-2phenylquinolin-4-yloxy)pyrrolidine2־carboxamide</td><td></td>

<td> 50</td><td> ^0 0 S^-Y</td><td> ethyl l-((2S,4R)-l-((S)-2-tert-butyl-4-(3,4dihydroquinolin-1 (2H)-yl)4־oxobutanoyI)-4-(7-methoxy-2phenylquinolin-4-yloxy)pyrrolidine-2ca1boxamido)-2vinylcyclopropanecarboxylate</td><td> * *</td>

<td> 51</td><td> ¾. ^y~\ Υ^-Λ^ο-^~ €®^νΌ/° 7 O Zp.</td><td> ethyl l-((2S,4R)-l-((S)-2-tert~butyl-4-(3,4dihydroisoquinolin-2( 1 H)-yl)-4oxobutanoyi)-47)־-methoxy-2phenylquinolin-4-yloxy)pyrTOlidine-2carboxamido)-2vinylcyclopropanecarboxylate</td><td> *</td>

<td> No.</td><td> STRUCTURE</td><td> Name</td><td> EC5C</td>

<td> 52</td><td> O/,. ΐ ^HN־ ^׳iC⁰״^H^/Νγ^Αο 0 a. ff</td><td> (2R.6R, 13aS, 14aR, 16aS,Z)-2־7)־methoxy2-phenylquinolin-4-yloxy)-5,16-dioxo-6(2-oxo2־-(piperidm~ l-yl)ethyl)- 1 ^,3,5,6,7,8,9,10,11.13a,14,14a,l 5,16,16ahexadecahydrocyclopropa[e]pyrrolo[ 1,2a][l ,4]diazacyclopentadecme-14acarboxylic acid</td><td></td>

<td> 53</td><td> o_/׳. /~~y ,p q Z\ X-_O^Z I 1 L HNI1.^ ° k/Ny-X-Ao f&gt; O *&gt; ff</td><td> (2R,6R,13aS,14aR,16aS,Z)-ethyl 2-(7methoxy-2-phenylquinolin-4-yloxy)-5,16dioxo-6-(2-oxo2־-(piperidin-l-yl)ethyl)- 3,5,6,7,8,9,10,11,13a,14,140,15,16,16ahexadec ahydrocyclopropa[e]pyrrolo[l ,2a][l ,4]diazacyclopentadecine-l 4acarboxylate</td><td></td>

<td> 54</td><td> O/_ C3^° ° ' 0 V. &gt;</td><td> (2R,6R, 13 aS, 14aR, 16aS,Z)-ethyl 6-(2-tertbutoxy-2-oxoethyl)-2-(7-methoxy-2phenylquinolm4־-yIoxy)-5,16-dioxoⁱ1,2,3,5,6,7,8,9,10,11,13a,14,14a,15,16,16ahexadecahydrocyclopropa[e]pyrrolo[ 1,2a] [ 1,4]diazacyclopentadeci11e-14acarboxylate</td><td> *</td>

<td> No.</td><td> STRUCTURE</td><td> Name</td><td> EC5</td>

<td> 55</td><td> 0/__ Cw° ° X /׳-- C 0 Ί&gt;</td><td> (2R,6R, 13aR, 14aS, 16aS,Z)-ethyI 6-(2-tertbutoxy'2-oxoethyl)-27)־-methoxy-2phenyiquinolin-4-yloxy)-5_t 16-dioxo1Λ3.5,6,7.8,9,10,11,130,14,14a,15,16,16ahexadecahydrocyclopropa[e]pyrrolo[ 1,2a][ 1,4]diazacyclopentadecme-14acarboxylate</td><td> *</td>

<td> 56</td><td> °&lt; /~~\ן. -^O Q JL^7־׳\ ^OH 0 ־ח^/ס</td><td> 1 -((2S.4R)־ I-((S)-2־tert-butyl-4-(3,4dihydroquinolin-1 (2H)-yl)-4oxobutanoyl)-4-(7־methoxy-2phenylquinolin-4-yloxy)pyrrolidine-2carboxamido)-2vinylcyclqpropanecaiboxylic acid</td><td> **</td>

<td> 57</td><td> o O ^XO ^o־S 0 54 O o □:</td><td> l-((2S,4R)-l-((S)2־-tert-butyl-4-(3,4dihydroisoquinolin-2(l H)-yl)-4oxobutanoyl)-4-(7 -methoxy-2phenylquinolin-4-yloxy)pyrrolidine-2carboxamido)-2vinylcyclopropanecaiboxylic acid</td><td> «</td>

<td> No.</td><td> STRUCTURE</td><td> Name</td><td> EC5C</td>

<td> 58</td><td> ״y f-^o Q W 0 O o X</td><td> (2R,6R,13aS,14aR,16aS,Z)־N(cyclopropylsulfonyI)-2-(7-methoxy-2phenylquinolin .4-yloxy)-5,16-dioxo2)-6־oxo-2-(piperidm- l-yl)ethyl)1,2,3,5,6,7,8,9,10,11,138,14,14a,15,16,16ahexadecahydrocyclopropa[e]py1T010[l ,2a][l ,4]diazacyclopentadecine-14acarboxamide</td><td> ***</td>

<td> 59</td><td> ״J χ.^ω-ο r° o X__________________</td><td> (2R,6R,13aR,14aS,16aS,Z)-N(cyclopropylsulfonyl)-2-(7-methoxy-2phenylquinolin-4-yloxy)-5,16-dioxo-6-(2oxo-2-(piperidin-1 -yl)ethyl)1,2,3,5,6,7,8,9,10,11,13a,14,14a,15,16,16ahexadecahydrocyclopropa[e]pyrrolo[ 1,2a][l,4]diazacyclqpentadecme-l 4acarboxamide</td><td> *Φ *</td>

<td> 60</td><td> °1 °;s?׳\C , O</td><td> (2S,4R)-1 -((S)-2-tert-butyl-4-oxo-4(piperidin-1 -yl)butanoyl)-N-(( 1R,2S)-1(cyc!opropylsulfonylcarbamoyl)-2vinylcyclqpropyl)-4-(7 -methoxy-2phenylquinolin-4-yloxy)pynolidme-2carboxamide</td><td></td>

Example 4. Assay for Identifying compounds which Inhibit HCV Replication

[0272] Compounds claimed herein are tested for the ability to inhibit viral replication of the Hepatitis C replicon in cultured cells in which the HCV replicon construct has been incorporated. The HCV replicon system was described by Bartenschlager, et. al (Science, 285, pp. 110-113 (1999)). The replicon system is predictive of in vivo anti-HCV activity; compounds that are active in humans uniformly evidence activity in the replicon assay.

[0223] In this assay HCV replicon containing cells are treated with different concentrations of the test compound to ascertain the ability of the test compound to suppress replication of the HCV replicon. As a positive control, HCV replicon&lt;-containing cells are treated with different concentrations of interferon alpha, a known inhibitor of HCV replication. The replicon assay system includes Neomycin Phosphotransferase (NPT) as a component of the replicon itself in order to detect the transcription of replicon gene products in the host cell. Cells in which the HCV replicon is actively replicating have high levels of NPT; the level of NPT is proportional to HCV replication. Cells in which the HCV replicon is not replicating also have low levels of NPT and thus do not survive when treated with Neomycin. The NPT level of each sample is measured using a captured ELISA.

[0224] A protocol for testing compounds for the ability to inhibit viral replication of the Hepatitis C replicon cultured cells in which the replicon construct has been incorporated, follows.

4A. HCV Replicon andRepIicon Expression

[0225] The HCV genome consists of a single ORF that encodes a 3000 amino acid polyprotein. The ORF is flanked on the 5' side by an untranslated region that serves as an internal ribosome entry site (IRES) and at the 3' side by a highly conserved sequence necessary for viral replication (3'-NTR). The structural proteins, necessary for viral infection, are located near the 5’ end of the ORF. The non-structural proteins, designated NS 2 to NS5B comprise the remainder of the ORF.

[0226] The HCV replicon contains, 5’-3׳, the HCV-IRES, the neomycin phosphotransferase (neo) gene, the IRES of encephalomyocarditis virus, which direct«? translation of HCV sequences NS3 to NS5B, and the 3’-NTR‘ ,The sequence of the HCV replicon has been deposited in GenBank (Accession no. AJ242652).

[0227] The replicon is transfected into Huh-7 cells using standard methods such as electroporation.

4B. Cell Maintenance

[0228] The equipment and materials include, but are not limited to, Huh-7 HCV repliconcontaining cells, maintenance media (DMEM (Dulbecco's modified Eagle media) supplemented with 10% FBS, L-glutamine, non-essential amino acids, penicillin (100 units/ml), streptomycin (100 micrograms/ml), and 500 micrograms/ml of Geneticin (G418), screening media (DMEM

PCT/US2007/0J6018 supplemented with 10% FBS, L-glutamine, non-essential amino acids, penicillin (100 units/ml) and streptomycin (100 micrograms/ml)), 96 well tissue culture plates (flat bottom), 96 well plates (U bottom for drug dilution), Interferon alpha for positive control, fixation reagent (such as methanol: acetone), primary antibody (rabbit anti-ΝΡΤΠ), secondary antibody: Eu-Nl 1, and enhancement solution.

[0229] HCV replicon-containing cells support high levels of viral RNA replicon replication when their density is suitable. Over-confluency causes decreased viral RNA replication. ׳Therefore, cells must be kept growing in log phase in the presence of 500 micrograms/ml of G418. Generally, cells should be passed twice a week at 1: 4-6 dilution. Cell maintenance is conducted aS follows:

[0230] HCV repl icon-confining cells are examined under a microscope to ensure that cells growing well. Cells are rinsed once with PBS and 2 ml trypsin is added. The cell/ trypsin mixture is incubated at 37 °C in a CO2 incubator for 3-5 minutes. After incubation 10 ml of complete media is added to stop the trypsinization reaction. Cells are blown gently, put into a 15 ml tube, and spun at 1200 rpm for 4 minutes. The trypsin/ medium solution is removed. Medium (5 ml) is added and the cells are mixed carefully. The cells are counted.

[0231] The cells are then seeded onto 96-well plates at a density of 60007500־ cells/100 microliters/ well (6-7.5 x 10^s cells/10 ml/plate). The plates are then incubated at 37 °C in a 5% CO2 incubator.

[0232] Cells are examined under a microscope approximated 24 hours after seeding and prior to »4ding drugs. If counting and dilution were performed correctly, cells are 6070%־ confluent and nearly all cells should attach and spread evenly in the well.

4C. Treatment ofHCV-replicon containing cells ־with Test Compound

[0233] HCV replicon-containing cells are rinsed with once PBS once; 2 mis of trypsin are then added. Cells are incubated at 37’C in a 5% CO2 incubator for 3-5 minutes. 10 mis of complete medium is added to stop the reaction. Cells are blown gently, put into a 15 ml tube, and spun at 1200 rpm for four minutes. The trypsin/medium solution is removed and 5 mis of medium (500 ml DMEM (high glucose)) from BRL catalog #12430-054; 50 mis 10% FBS, 5% Geneticin G418 (50 mg/ml, BRL catalog #10131-035), 5 ml MEM non-essential amino acids (lOOx BRL #11140-050) and 5 ml pen-strep (BRL #15140-148) is added. The cells and media are mixed carefully

[0234] Cells are plated with screening medium (500 ml DMEM (BRL #21063-029), 50 ml FBS (BRL #10082-147) and 5 ml MEM non-essential amino acid (BRL #11140-050) at 6000-7500 fcells/100 μΙ/well of 96 well plate (6-7.5x105 cells/10 ml/plate). Plates are placed into 37’C 5% CO3 incubator overnight

4D. Assay

[0235] The following morning, drugs (test compounds or interferon alpha) are diluted in 96 well U bottom plates with media or DMSO/media, depending on the final concentration chosen for screening. Generally for 6 concentrations of each test compounds ranging from 10 micromolar to 0.03 micromoiar are applied. 100 pl of the test compound dilution is placed in wells of the 96 well plate containing the HCV replicon cells. Media without drug is added to some wells as a negative controls. DMSO is known to affect cell growth. Therefore, if drugs diluted in DMSO are used, all wells, including negative control (media only) and positive control (interferon alpha) wells, must contain the same concentration of DMSO, for single dose screening. The plates are incubated at 37*C in a humidified 5% COj environment for three days.

[0236] On day four, the ΝΤΡΠ assay is quantitated. The medium is poured from the plates and the plates are washed once in 200 μΐ of PBS. The PBS is then decanted and the plates tapped in a paper towel to remove any remaining PBS. Cells are fixed in situ with 100 μΙ/well of pre-cooled (-20*C) methanol: acetone (1:1) and the plates are placed at -20'C for 30 minutes.

[0237] The fixing solution is poured from the plates and the plates allowed to air-dry completely (approximately one hour). The appearance of the dried cell layer is recorded and the density of the cells in the toxic wells is scored with the naked eye. Alternatively cell viability may be assessed using the MTS assay described below.

[0238] The wells are blocked with 200 μΐ of blocking solution (10% FBS; 3% NGS in PBS) for 30 minutes at room temperature. The blocking solution is removed and 100 μΐ of rabbit anti-ΝΡΤΠ diluted 1:1000 in blocking solution is added to each well. The plates are then incubated 45-60 minutes at room temperature. After incubation, wells are washed six times with PBS-0.05% Tween-20 solution. 100 gl of 1:15,000 diluted Europium (EU)-conjugated goat antirabbit in blocking buffer is added to each well and incubated at room temperature for 30-45 minutes. The plates are washed again and 100 μΐ of enhancement solution (Perkin Elmer #4001 0010) is added to each well. Each plate is shaken (approx. 30 rpm) in a plate shaker for three minutes. 95 pl is transferred from each well to a black plate; the EU signal is quantitated in a Perkin-Elmer VICTOR plate reader (EU-Lance).

[0239] Compounds shown in Example 3 have been tested in this assay. Those with one ״*” in the EC50 column have an EC50 greater than 10 micromolar, those with two^,s (**) have an EC50 of greater than 1 micromolar but less than 10 micromolar, and compounds with three’s (*.») have EC50 values of 100 nanomolar or less.

Example 5. Cytotoxicity Assays

[0240] To insure that the decrease in replicon replication is due to compound activity »gainst the HCV replicon rather than nonspecific toxicity assays are used to quantitate compound cytotoxicity.

5j4. Cellular protein albumin assay for cytotoxicity

[0241] Cellular protein albumin measurements provide one marker of cytotoxicity. The protein levels obtained from cellular albumin assays may also be used to provide a normalization reference for antiviral activity of compounds. In the protein albumin assay HCV repliconcontaining cells are treated for three days with different concentrations of helioxanthin; a compound that is known to be cytotoxic at high concentrations. The cells are lysed and the cell lysate used to bind plate-bound goat anti-albumin antibody at room temperature (25 °C to 28 °C) for 3 hours. The plate is then washed 6 times with IX PBS. After washing away the unbound proteins, mouse monoclonal anti-human serum albumin is applied to bind the albumin on the plate. The complex is then detected using phosphatase-labeled anti-mouse IgG as a second antibody.

5B. MTS Assay for Cytotoxicity

[0242] Cell viability may also be detennined by CELLTITER 96 AQUEOUS ONE Solution Cell Proliferation Assay (Promega, Madison WI), a colorimetric assay for determining the number of viable cells. In this method, before fixing the cells, 10-20 pl MTS reagent is added to each well acconiing to manufacturer's instructions, plates are incubated at 37 C and read at OD 490 תזיי. During the incubation period living cells covert the MTS reagent to a formazan product which absorbs at 490 nm. Thus the 490 nm absorbance is directly proportional to the number of living cells in culture.

[0243] A direct comparison of the Cellular Albumin and MTS methods for determining cytotoxicity may be obtained as follows: Cells are treated with different concentrations of test compound or Helioxanthin for a three day-period. Prior to lysis for detection albumin as described above, the MTS reagent is added according to manufacturer's instruction to each well and incubate at 37 °C and read at OD 490 run. The cellular albumin quantitation is then performed as described above.