Animal feeds comprising yeast glucan and feed process for growth of animals based on feeds comprising yeast glucan
Abstract
A composition and a process for enhancing animal growth by orally administering the animal with the composition are provided. The composition comprises an animal feed and a yeast glucan wherein the animal feed can contain at least one starch-bearing substance such as, for example, grain meal; at least one protein-bearing substance such as, for example, fish meal; a fat-containing substance such as, for example, soybean oil; and the yeast glucan can be obtained from a yeast such as, for example, Saccharomyces cerevisiae.

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3 claims: 3 independent, 0 dependent
- 1Eljárás állatok növekedésének gyorsítására, azzal jellemezve , hogy az állatokat olyan kompozíciókkal etetjük, amelyek takarmány mellett az állatok növekedési ütemének legalább mintegy 2 %-os fokozásához elegendő mennyiségben valamilyen élesztőglükánt tartalmaznak. First A method of accelerating the growth of an animal comprising feeding the animal with a composition comprising an amount of a yeast glucan in an amount sufficient to increase the growth rate of the animal by at least about 2%. 2. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy az állatok növekedési ütemét legalább mintegy 4 %-kal fokozzuk. Second The method of claim 1, wherein the growth rate of the animals is increased by at least about 4%. 3. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy az állatok növekedési ütemét legalább mintegy 6 %-kal fokozzuk. Third The method of claim 1, wherein the growth rate of the animals is increased by at least about 6%. 4. Az 1. igénypont szerinti eljárás, azzal jelleme zve , hogy az állatokat olyan kompozíciókkal etetjük, (mintegy amelyek az élesztőglükánt!5θ-2500 g/t mennyiségben tartalmazzák. 4th The method of claim 1, wherein the animals are fed with compositions (about 5g to 2500g / t of yeast glucan). 5. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy az állatokat olyan kompozíciókkal etetjük, amelyek az élesztőglükánt mintegy 100-2000 g/t mennyiségben tartalmazzák. 5th The method of claim 1, wherein the animals are fed compositions containing yeast glucan in an amount of about 100 to about 2000 g / t. 6. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy az állatokat olyan kompozíciókkal etetjük, amelyek az élesztőglükánt 200-1500 g/t mennyiségben tartalmazzák. 6th The method of claim 1, wherein the animals are fed with compositions containing 200-1500 g / t of yeast glucan. • · ···· ·· ···· • · * · « · · • · · · · · • ····· · · · • · · · ·· ·· · • · ···· ·· ···· • · * · « · · • · · · · · • ····· · · · • · · · ·· ·· · 7. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy az élesztőglükánt tartalmazó kompozíciókkal csirkéket, pulykákat, sertéseket vagy borjakat etetünk. 7th The method of claim 1, wherein the yeast glucan-containing compositions are used to feed chickens, turkeys, pigs or calves. 8. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy az élesztőglükánt tartalmazó kompozíciókkal választási malacokat etetünk. 8th The method of claim 1, wherein the yeast glucan containing compositions are fed to piglets of choice. 9. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy olyan élesztőglükánt alkalmazunk, amely mintegy 40-90 m^-ban ^>(1-3) és (3(1-6) kötéseket tartalmazó glükánokból áll. 9th The process of claim 1, wherein the yeast glucan is composed of glucans having from about 40 to about 90 (1-3) and (3 (1-6)) bonds. 10. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy olyan élesztőglükánt alkalmazunk, amely Saccharomyces cerevisiae, Saccharomyces uvarum, Candida utilis, Kluyveromyces fragilis és/vagy Pichia pastoris élesztőgombákból származik. 10th The method of claim 1, wherein the yeast glucan is derived from yeasts of Saccharomyces cerevisiae, Saccharomyces uvarum, Candida utilis, Kluyveromyces fragilis and / or Pichia pastoris. 11. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy olyan élesztőglükánt alkalmazunk, amely Saccharomyces cerevisiae élesztőgombákból származik. 11th A process according to claim 1 wherein the yeast glucan is derived from yeasts of Saccharomyces cerevisiae. 12. Az 1. igénypont szerinti eljárás, azzal jelleme zve , hogy olyan élesztőglükánt alkalmazunk, amelyet úgy állítunk elő, hogy (1) lúgos oldatban élesztőt szuszpendálva olyan elegyet készítünk, amely élesztőgombák sejtfalaiból álló, oldhatatlan frakciót tartalmaz;12th The process of claim 1, wherein the yeast glucan is prepared by (1) suspending the yeast in an alkaline solution to form a mixture comprising an insoluble fraction of yeast cell walls;
- 2(2) az elegyben levő, élesztőgombák sejtfalaiból álló oldhatatlan frakciót elkülönítjük; (2) separating the insoluble fraction of the yeast cell walls in the mixture; • · · · • · · · - 54 (5) az elválasztott, élesztőgombák sejtfalaiból álló, oldhatatlan frakciót savas oldatban szuszpendálva élesztőglükánt képezünk; és (4) az élesztőglükánt a savas oldattól elkülönítjük. - 54 (5) suspending the insoluble fraction of separated yeast cell walls in an acidic solution to form a yeast glucan; and (4) separating the yeast glucan from the acidic solution. 15. A 12. igénypont szerinti eljárás, azzal jellemezve , hogy lúgos oldatként nátrium-hidroxid-oldatot alkalmazunk. 15th The process according to claim 12, wherein the alkaline solution is sodium hydroxide solution. 14. A 12. igénypont szerinti eljárás, azzal jellemezve , hogy az élesztőszuszpenziót Saccharomyces cerevisiae élesztőgombákból állítjuk elő. 14th The process according to claim 12, wherein the yeast suspension is prepared from yeasts of Saccharomyces cerevisiae. 15. Az 1. igénypont szerinti eljárás, azzal jellemezve , hogy az állatokat olyan kompozícióval etetjük, amely a megadott komponensek mellett még viz- és zsiroldható vitaminokból, valamint nyomelemekből álló vitamin/ásványi anyag elegyet is tartalmaz. 15th The method of claim 1, wherein the animals are fed with a composition comprising, in addition to the components indicated, a vitamin / mineral mixture of water and fat soluble vitamins and trace elements. 16. A 15. igénypont szerinti eljárás, azzal jellemezve , hogy olyan vitamin/ásványi anyag elegyet alkalmazunk, amely A-vitaminból, C-vitaminból, D-vitaminból, E-vitaminból, K-vitaminból, B-^-vitaminból, riboflavinból, pantoténsavból, nikotinsavból, fólsavból, biotinból, rézből, cinkből, jódból, szelénből, mangánból, vasból és/vagy a felsorolt elemek sóiból áll. 16th The method of claim 15, wherein the vitamin / mineral mixture is selected from the group consisting of vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, vitamin B-4, riboflavin, pantothenic acid, nicotinic acid, folic acid, biotin, copper, zinc, iodine, selenium, manganese, iron and / or salts of the listed elements. 17. Eljárás állatok takarmányozására takarmányt és élesztőglükánt tartalmazó kompozíciókkal, azzal jellemezve , hogy olyan kompozíciókat alkalmazunk, amelyekben a takarmány kukoricaőrleményből, kukoricalisztből, zablisztből, szójalisztből, egész kukoricaszemekből és/vagy búzalisztből álló keményi- :17th A method for feeding animals with compositions comprising feed and yeast glucan, wherein the feed comprises compositions comprising starch consisting of cornmeal, cornmeal, oatmeal, soybean meal, whole corn kernels and / or wheat flour: - 35 carrier materials consisting of whey powder, fishmeal, soybean meal, soybean protein concentrate, soybean meal, blood protein, plasma milk, skimmed milk powder, whey protein yeast, canola flour, corn flour and cornmeal, cornflour and cornflour fat, fat, pig fat, tallow, soya oil, lecithin, coconut fat and / or whey fat, contains a mixture of antibiotics and a mixture of vitamins and minerals, and the yeast glucan is derived from the yeasts Saccharomyces cerevisiae, Saccharomyces uvarum, Candida utilis, Kluyveromyces fragilis and / or Pichia pastoris. - 35 tőhordozó anyagot, savóporból, hallisztből, szójalisztből, szójafehérje-koncentrátumból, szójabablisztből, vérlisztből, vérplazmafehérjéből, fölözött tejből készített tejporból, savófehér je- konc ént r át umból, kanolalisztből, kukoricasikér-lisztből, buzasikérlisztből, élesztőből és/vagy napraforgólisztből álló fehérjehordozó anyagot, disznózsirból, faggyúból, szójaolajból, lecitinből, kókuszzsirból és/vagy savózsiradék-elégyből álló zsírtartalmú anyagot, antibiotikumelegyet és vitaminokból, valamint ásványi anyagokból álló elegyet tartalmaz, az élesztőglükán pedig Saccharomyces cerevisiae, Saccharomyces uvarum, Candida utilis, Kluyveromyces fragilis és/vagy Pichia pastoris élesztőgombákból származik. 18. A 17. igénypont szerinti eljárás, azzal jellemezve , hogy olyan kompozíciókat alkalmazunk, amelyekben a keményítőhordozó anyag kukoricaőrlemény, zabliszt, zabdara és/vagy szójababliszt;a fehérjehordozó anyag halliszt és/vagy szójababliszt;a zsiradéktartalmu anyag savózsiradék-elegy és az élesztőglükán Saccharomyces cerevisiae élesztőgombákból származik. 18th The method of claim 17 wherein the starch carrier is cornmeal, oatmeal, oatmeal and / or soybean flour;the protein carrier is fish meal and / or soybean meal;the fat-containing material is a mixture of whey fat and yeast glucan is derived from yeasts of Saccharomyces cerevisiae. 19. A 17· igénypont szerinti eljárás, azzal jellemezve , hogy olyan kompozíciókat alkalmazunk, amelyek takarmányként 0,001-30 zablisztet, 0,001-30 m% zabdarát, 0,001-70 m% kukoricaőrleményt, 0,001-15 hallisztet, 0,001-40 m% szójabablisztet, 0,001-8 n$> szójahüvely-arleményt, 0,001-40 savóport, 0,1-40 búzadarát, 0,001-30 m% savózsiradék-elegyet, 0,001-5 dikalcium-foszf átot, 0,001-4 kalcium-karbonátot, 0,001-4 fumársavat, 0,001-3 mennyiségű, 1 tonna kompozícióra vonatkoztatva 100 g klór-tetraciklinből, 100 g szulfáté• · • ····· · « · ··· · ·· ·· · 19th The process according to claim 17, wherein the compositions are selected from the group consisting of 0.001 to 30% oat flour, 0.001 to 30% oatmeal, 0.001 to 70% corn flour, 0.001 to 15 fish meal, 0.001 to 40% soybean flour, 0.001 to 40% feed. Soybean pods, 0.001-40 whey powder, 0.1-40 wheat flour, 0.001-30 m% whey fat mixture, 0.001-5 dicalcium phosphate, 0.001-4 calcium carbonate, 0.001-4 fumaric acid, 0.001 -3 amount, 1 per tonne of composition per 100 g of chloro-tetracycline, 100 g of sulfate · · ············· - 36 tazinból és 50 g penicillinből álló antibiotikumélegyet, 0,001-1 vajat, 0,001-1 m% mennyiségű, juharfa besűrített nedvéből álló izanyagot, 0,001-1 lizin-hidrokloridot és 0,001-2 m% mennyiségű, réz-szulfátból, kolinból, szelénből, E-vitaminból, biotinból, fólsavból, glicinből és etoxikinből álló vitamin/ásványi anyag elegyet, továbbá tonnánként 200-1500 g élesztőglükánt tartalmaznak. - a mixture of antibiotics consisting of 36 tazine and 50 g penicillin, 0.001 to 1 butter, 0.001 to 1% by weight of maple tree sap, 0.001 to 1% lysine hydrochloride and 0.001 to 2% by weight of copper sulphate, choline, selenium, They contain a vitamin / mineral mixture of vitamin E, biotin, folic acid, glycine and ethoxyquin and 200-1500 g per tonne of yeast glucan. 20. A 17. igénypont szerinti eljárás, azzal jellemezve , hogy olyan kompozíciókat alkalmazunk, amelyek mintegy 10-80 keményít őhordozó anyagot, mintegy 10-50 uffa fehérjehordozó anyagot, mintegy 2-15 mennyiségű zsiradéktartalmu anyagot és tonnánként mintegy 50-2500 g élesztőglükánt tartalmaznak. 20th The method of claim 17, wherein the compositions comprise from about 10 to about 80 starch carriers, from about 10 to about 50 uffa protein carriers, from about 2 to about 15 fats, and from about 50 to about 2500 g of yeast glucan per tonne. 21. A 17. igénypont szerinti eljárás, azzal jellemezve , hogy olyan kompozíciókat alkalmazunk, amelyek mintegy 20-40 m% keményitőhordozó anyagot, mintegy 18-30 m% fehérjehordozó anyagot, mintegy 6-12 m% mennyiségű zsiradéktartalmu anyagot és tonnánként 200-1500 g élesztőglükánt tartalmaznak. 21st The method of claim 17, wherein the compositions comprise about 20-40% m / m starch carrier material, about 18-30% m / m proteinaceous material, about 6-12% m / m fat content and 200-1500 g / yg glucan. They contain. 22. Eljárás választási malacok takarmányozására, azzal jellemezve , hogy a választási malacokat olyan kompozíciókkal etetjük, amelyek takarmányként 0,001-30 zablisztet, 0,001-30 zabdarát, 0,001-70 m% kukoricaőrleményt, 0,001-15 hallisztet, 0,001-40 m% szójabablisztet, 0,001-8 szójahüvely-őrleményt, 0,001-40 savóport, 0,1-40 m% búzadarát, 0,001-30 m% savózsiradék-elegyet, 0,001-5 m% dikalcium-foszfátot, 0,001-4 m% kalcium-karbonátot, 0,001-4 m% fumársavat, 0,001-3 mennyiségű, 1 tonna kompozícióra vonatkoztatva • · ··· · 22nd A method for feeding weaning piglets, comprising feeding the weaning piglets with compositions comprising 0.001 to 30 oat flour, 0.001 to 30 oat flour, 0.001 to 70% corn meal, 0.001 to 15 fish meal, 0.001 to 40% soybean meal, 0.001 to 8%. soybean meal, 0.001 to 40% whey powder, 0.1 to 40% wheat flour, 0.001 to 30% whey fat mixture, 0.001 to 5% dicalcium phosphate, 0.001 to 4% calcium carbonate, 0.001 to 4% fumaric acid, from 0.001 to 3, 1 per tonne of composition • · ··· · - 57 100 g klór-tetraciklinből, 100 g szulfametazinból és 50 g penicillinből álló antibiotikumelegyet, 0,001-1 m% vajat, 0,001-1 m% mennyiségű, juharfa besűrített nedvéből álló izanyagot, 0,001íhidro - 57 antibiotic mixtures of 100 g of chlorotetracycline, 100 g of sulfamethazine and 50 g of penicillin, 0,001 to 1% butter, 0,001 to 1% by weight of concentrated maple juice, -1 m% liziíAkloridot és 0,001-2 m% mennyiségű, réz-szulfátból, kolinból, szelénből, E-vitaminból, biotinból, fólsavból, glicinből, valamint etoxikinből álló vitamin/ásványi anyag elegyet, továbbá tonnánként 200-1500 g élesztőglükánt tartalmaznak· Contains -1% w / w Lysyl chloride and 0.001% to 2% w / v of a mixture of copper sulfate, choline, selenium, vitamin E, biotin, folic acid, glycine and ethoxyquin and 200-1500 g / yeast glucan. 25. Takarmányt és élesztőglükánt tartalmazó kompozíciók, azzal jellemezve , hogy annyi élesztőglükánt foglalnak magukban, amennyi elegendő ahhoz, hogy a kompozíciókkal etetett állatok fejlődési ütemét legalább mintegy 2 %-kal növelje. 25th Compositions containing feed and yeast glucan, characterized in that they contain enough yeast glucan to increase the rate of development of the animals fed with the compositions by at least about 2%. 24· A 25. igénypont szerinti kompozíciók, azzal jellemezve , hogy annyi élesztőglükánt tartalmaznak, amennyi elegendő a kompozíciókkal etetett állatok fejlődési ütemének legalább mintegy 4 %-os növeléséhez. Compositions according to claim 25, characterized in that they contain enough yeast glucans sufficient to increase the rate of development of the animals fed with the compositions by at least about 4%. 25. A 25. igénypont szerinti kompozíciók, azzal jellemezve , hogy annyi élesztőglükánt tartalmaznak, amennyi elegendő a kompozíciókkal etetett állatok fejlődési ütemének legalább mintegy 6 %-os növeléséhez. 25th Compositions according to claim 25, characterized in that they contain enough yeast glucans sufficient to increase the rate of development of the animals fed with the compositions by at least about 6%. 26. A 25· igénypont szerinti kompozíciók, azzal jellemezve , hogy tonnánként mintegy 50-2500 g élesztőglükánt tartalmaznak· 26th Compositions according to claim 25, characterized in that they contain from about 50 g to about 2500 g per ton of yeast glucan. 27. A 25· igénypont szerinti kompozíciók, azzal jellemezve , hogy tonnánként mintegy 100-2000 g élesztőglükánt tartalmaznak. 27th Compositions according to claim 25, characterized in that they contain from about 100 to about 2000 g of yeast glucan per tonne. 28. A 25. igénypont szerinti kompozíciók, azzal j e 1 1 e - • · » · · · • ····· · · · «·· · ·· · · · 28th Compositions as claimed in claim 25, wherein: -38m e z v e , hogy tonnánként 200-1500 g élesztőglükánt tartalmaznak. -38m, which contains 200-1500 g of yeast glucan per tonne. 29. A 23· igénypont szerinti kompozíciók, azzal jellemezve , hogy csirkék, pulykák, sertések és borjak takarmányozáséira alkalmazhatók. 29th Compositions according to claim 23, characterized in that they are used in the feeding of chickens, turkeys, pigs and calves. 30. A 23. igénypont szerinti kompozíciók, azzal jellemezve , hogy választási malacok takarmányozására alkalmazhatók. 30th Compositions according to claim 23, characterized in that they are used for feeding piglets. 31. A 23. igénypont szerinti kompozíciók, azzal jellemezve , hogy élesztőglükánként mintegy 40-90 m%-ban p(l-3) és ^(1-6) kötéseket tartalmazó glükánokból álló elegyet foglalnak magukban. 31st Compositions according to claim 23, characterized in that they comprise a mixture of glucans containing from about 40% to about 90% by weight of yeast glucans having p (1-3) and (1-6) linkages. 32. A 23· igénypont szerinti kompozíciók, azzal jellemezve , hogy Saccharomyces cerevisiae, Saccharomyces uvarum, Candida utilis, Kluyveromyces fragilis és/vagy Pichia pastoris élesztőgombákból származó élesztőglükánt tartalmaznak. 32nd Compositions according to claim 23, characterized in that they contain yeast glucan from the yeasts Saccharomyces cerevisiae, Saccharomyces uvarum, Candida utilis, Kluyveromyces fragilis and / or Pichia pastoris. 33. A 23. igénypont szerinti kompozíciók, azzal jellemezve , hogy Saccharomyces cerevisiae élesztőgombákból származó élesztőglükánt tartalmaznak. 33rd Compositions according to claim 23, characterized in that they contain yeast glucan from yeasts of Saccharomyces cerevisiae. 34. A 23. igénypont szerinti kompozíciók, azzal jellemezve, hogy víz- és zsirolclható vitaminokból és nyomelemekből álló vitamin/ásványi anyag elegyet is tartalmaznak. 34th Compositions according to claim 23, characterized in that they also contain a vitamin / mineral mixture of water and fat-soluble vitamins and trace elements. 35. A 34. igénypont szerinti kompozíciók, azzal jellemezve , hogy olyan vitamin/ásványi anyag elegyet tartalmaznak, amely A—vitaminból, C—vitaminból, D—vitaminból, E—vitamin— • · ··«· ·· · ··· • · · « · · · • · · · · · * ·«··· · · · «·· · ·« ·· · 35th Compositions according to claim 34, characterized in that they contain a vitamin / mineral mixture consisting of Vitamin A, Vitamin C, Vitamin D, Vitamin E. «· · · *« «« · · · · · · - 39 ból, K-vitaminból, B-^-vitaminból, riboflavinból, pántoténsavból, nikotinsavból, fólsavból, biotinból, rézből, cinkből, jódból, szelénből, mangánból, vasból és/vagy a felsorolt elemek sóiból áll. - 39, Vitamin K, Vitamin B - riboflavin, ribotenic acid, nicotinic acid, folic acid, biotin, copper, zinc, iodine, selenium, manganese, iron and / or salts of the listed elements. 36. A 23. igénypont szerinti kompozíciók, azzal jellemezve, hogy olyan élesztőglükánt tartalmaznak, amelyet úgy állítottunk elő, hogy (1) lúgos oldatban élesztőt szuszpendálva olyan elegyet készítettünk, amely élesztőgombák sejtfalaiból álló, oldhatatlan frakciót tartalmazott;36th Compositions according to claim 23, characterized in that they contain a yeast glucan prepared by (1) suspending the yeast in an alkaline solution to form a mixture containing an insoluble fraction of yeast cell walls;(2) az elegyben levő, élesztőgombák sejtfalaiból álló oldhatatlan frakciót elkülönítettük;(2) isolating the insoluble fraction of the yeast cell walls in the mixture;
- 3(3) az elválasztott, élesztőgombák sejtfalaiból álló, oldhatatlan frakciót savas oldatban szuszpendálva élesztőglükánt képeztünk; és (4) az élesztőglükánt a savas oldattól elkülönítettük. (3) suspending the insoluble fraction of separated yeast cell walls in an acidic solution to form a yeast glucan; and (4) isolating the yeast glucan from the acidic solution. 37. A 3θ· igénypont szerinti kompozíciók, azzal jellemezve, hogy olyan élesztőglükánt tartalmaznak, amelynek előállításához lúgos oldatként vizes nátrium-hidroxid-oldatot használtunk fel. 37th Compositions according to claim 3θ, characterized in that they contain a yeast glucan prepared as an aqueous solution of sodium hydroxide as an alkaline solution. 38. A 36. igénypont szerinti kompozíciók, azzal jellemezve, hogy olyan élesztőglükánt tartalmaznak, amelynek előállításához Saccharomyces cerevisiae élesztőgombákat használtunk fel. 38th Compositions according to claim 36, characterized in that they contain a yeast glucan prepared from yeasts of Saccharomyces cerevisiae. 39 «Compositions consisting of fodder and yeast glucan, characterized in that, as fodder from corn flour, corn flour, oat flour, soybean flour, 39« Kompozíciók, amelyek takarmányból és élesztőglükánból állnak, azzal jellemezve , hogy takarmányként kukoricaőrleményből, kukoricalisztből, zablisztből, szójabablisztből, i • ·· · • ·· · - 40 - ......... - 40 - ......... egész kukoricaszemekből és/vagy búzalisztből álló keményitőhordozó anyagot, savóporból, hallisztből, szójalisztbol, szójafehérje-koncentrátumból, szójabablisztből, vérlisztből, vérplazmafehérjéből, fölözött tejből készített tejporból, savófehérjekoncentrátumból, kanolalisztből, kukoricasikér-lisztből, buzasikérlisztből, élesztőből és/vagy napraforgólisztből álló fehérjehordozó anyagot, disznózsírból, faggyúból, szójaolajból, lecitinből, kókuszzsirból és/vagy savózsiradék-elégyből álló zsírtartalmú anyagot, antibiotikumelegyet és vitaminokból, valamint ásványi anyagokból álló elegyet tartalmaznak olyan élesztőglükán mellett, amely Saccharomyces cerevisiae, Saccharomyces uvarum, Gandida utilis, Kluyveromyces fragilis és/vagy Pichia pastoris élesztőgombákból származik· wholemeal starch, whey powder, whey powder, whey powder, whey powder, whey powder, whey powder, whey powder, whey powder, lard, tallow, soybean oil, lecithin, containing coconut fat and / or serum fat, a mixture of antibiotics and a mixture of vitamins and minerals in addition to yeast glucan from Saccharomyces cerevisiae, Saccharomyces uvarum, Gandida utilis, Kluyveromyces or Plu 40. A 59. igénypont szerinti kompozíciók, azzal jellemezve , hogy keményít őhordozó anyagként kukoricaőrleményt, zablisztet, zabdarát és/vagy szójabablisztet, fehérjehordozó anyagként hallisztet és/vagy szójabablisztet, zsiradéktartalmu anyagként savózsiradék-elegyet tartalmaznak Saccharomyces cerevisiae élesztőgombákból származó élesztőglükán mellett. 40th Compositions according to Claim 59, characterized in that the starch carrier is corn flour, oat flour, oat flour and / or soybean flour, the protein carrier is fish flour and / or soybean flour, and the fat-containing substance is a mixture of wheat grease and saccharomyces. 41. A 59· igénypont szerinti kompozíciók, azzal jellemezve , hogy takarmányként 0,001-50 m% zablisztet, 0,001-50 zabdarát, 0,001-70 kukoricaőrleményt, 0,001-15 ni% hallisztet, 0,001-40 m% szójabablisztet, 0,001-8 m% szójahüvely-őrleményt, 0,001-40 m% savóport, 0,1-40 búzadarát, 0,001-50 m% savózsiradék-elegyet, 0,001-5 dikalcium-foszfátot, 0,001-4 kalcium-karbonátot, 0,001-4 m% fumársavat, 0,001-5 mennyiségű, 1 tonna kompozícióra vonatkoztatva 100 g klór-tetraciklinből, 100 g szulfametazinból, valamint 50 g penicillinből álló antibiotikumelegyet, 0,001—1 m% vajat, 0,001—1 m/S mennyiségű, 41st Compositions according to claim 59, characterized in that the feed comprises 0.001-50% by weight of oat flour, 0.001-50% by weight of oatmeal, 0.001-70% by weight of corn flour, 0.001- 15% by weight of soybean flour, 0.001-8% by weight of soybean meal. millet, 0.001 to 40% whey powder, 0.1 to 40% wheat flour, 0.001 to 50% whey fat mixture, 0.001 to 5 dicalcium phosphate, 0.001 to 4 calcium carbonate, 0.001 to 4% fumaric acid, 0.001 to 5 , Per tonne of composition, 100 g of chlorotetracycline, 100 g of sulfamethazine, and a mixture of 50 g of penicillin antibiotic, 0.001-1 m% butter, 0.001-1 m / S, - 41 • · ···· * * · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · lysine hydrochloride and 0.001 to 2% m / m of a mixture of copper sulfate, choline, selenium, vitamin E, biotin, folic acid, glycine and ethoxyquin, and 200- They contain 1500 g of yeast glucan. - 41 • · ···· *· ···· * · · · · * · « · t · · · ·«····«« * ··· ···«·» * r juharfa besűrített nedvéből álló izanyagot, 0,001-1 m% lizin-hidrokloridot, valamint 0,001-2 m% mennyiségű, réz-szulfátból, kolinból, szelénből, E-vitaminból, biotinból, fólsavból, glicinből és etoxikinből álló vitamin/ásványi anyag elegyet, továbbá tonnánként 200-1500 g élesztőglükánt tartalmaznak. 42. A 39. igénypont szerinti kompozíciók, azzal jellemezve , hogy mintegy 10-80 keményítőhordozó anyagot, mintegy 10-50 m% fehérjehordozó anyagot, mintegy 2-15 mennyiségű zsiradéktartalmu anyagot és tonnánként mintegy 50-2500 g élesztőglükánt tartalmaznak. 42nd Compositions according to claim 39, characterized in that they contain from about 10 to about 80 starch carriers, from about 10 to about 50 wt% protein carriers, from about 2 to about 15 greases and from about 50 to about 2500 g of yeast glucan per ton. 43· A 39· igénypont szerinti kompozíciók, azzal jellemezve , hogy mintegy 20-40 m% keményítőhordozó anyagot, mintegy 18-30 vfá fehérjehordozó anyagot, mintegy 6-12 m% mennyiségű zsiradéktartalmu anyagot és tonnánként 200-1500 g élesztőglükánt tartalmaznak· Compositions according to claim 39, characterized in that they contain from about 20% to about 40% starch carrier material, from about 18% to about 30% proteinaceous material, from about 6% to about 12% fat content and from about 200% to about 1500g / ton yeast glucan. 44. Kompozíciók, azzal jellemezve , hogy 0,001-30 m% zablisztet, 0,001-30 m% zabdarát, 0,001-70 m% kukoricaőrleményt, 0,001-15 m% hallisztet, 0,001-40 m% szójabablisztet, 0,001-8 m% szójahüvely-őrleményt, 0,001-40 m% savóport, 0,1-40 m% búzadarát, 0,001-30 m% savózsiradék-elegyet, 0,001-5 dikalcium-foszfátot, 0,001-4 m% kalcium-karbonátot, 0,001-4 m% fumársavat, 0,001-3 vfá mennyiségű, 1 tonna kompozícióra vonatkoztatva 100 g klór-tetraciklinből, 100 g szulfametazinból, valamint 50 g penicillinből álló antibiotikumélegyet, 0,001-1 m% vajat, 0,001-1 m% mennyiségű, juharfa besűrített nedvéből álló izanyagot, \hidro 44th Compositions characterized in that 0.001 to 30% by weight of oatmeal, 0.001 to 30% by weight of oatmeal, 0.001 to 70% by weight of corn flour, 0.001 to 15% by weight of fishmeal, 0.001 to 40% by weight of soybean meal, 0.001 to 8% by weight of soybean meal, 0.001 to 40% whey powder, 0.1 to 40% wheat grout, 0.001 to 30% whey fat mixture, 0.001 to 5% dicalcium phosphate, 0.001 to 4% calcium carbonate, 0.001 to 4% fumaric acid, 0.001 to 3 vfa of 100 g of chlorotetracycline, 100 g of sulfamethazine per tonne of composition, and a mixture of 50 g of penicillin antibiotic, 0.001 to 1% butter, 0.001 to 1% by weight of maple tree sap, 0,001-1 m% lizin-^kloridot és 0,001-2 m% mennyiségű, réz-szulfátból, kolinból, szelénből, B-vitaminból, biotinból, fólsavból, glicinből, valamint etoxikinből álló vitamin/ásványi anyag ele- 0.001 to 1% by weight of lysine chloride and 0.001 to 2% by weight of a vitamin / mineral element consisting of copper sulfate, choline, selenium, vitamin B, biotin, folic acid, glycine and ethoxyquin. They contain about 42 to 15 grams of yeast glucan per tonne. Λ · r*i r- 42 gyet, továbbá tonnánként 200-1500 g élesztőglükánt tartalmaznak. 45. Kompozíciók, azzal jellemezve, hogy 0,001-50 zablisztet, 0,001-30 zabdarát, 0,001-70 kukoricaőrleményt, 0,001-15 hallisztet, 0,001-40 m% szójabablisztet, 0,001-8 n$ szó jahüvely-őrleményt, 0,001-40 m% savóport, 0,1-40 m% búzadarát, 0,001-30 m% savózsiradék-elegyet, 0,001-5 dikalcium-f oszf át ot, 0,001-4 kalcium-karbonátot, 0,001-4 fumársavat, 0,001-3 mennyiségű, 1 tonna kompozícióra vonatkoztatva 100 g klór-tetraciklinből, 100 g szulfametazinból, valamint 50 g penicillinből álló antibiotikumélegyet, 0,001-1 m% vajat, 0,001-1 mennyiségű, juharfa besűrített nedvéből álló izanyagot, 0,001-1 m% lizin-hidrokloridot és 0,001-2 mennyiségű, réz-szulfátból, kolinból, szelénből, E-vitaminból, biotinból, fólsavból, glicinből, valamint etoxikinből álló vitamin/ ásványi anyag elegyet, továbbá tonnánként 200-1500 g mennyiségben Saccharomyces cerevisiae élesztőgombákból előállított élesztőglükánt tartalmaznak. 45th Compositions characterized in that 0.001 to 50% oatmeal, 0.001 to 30 oatmeal, 0.001 to 70 cornmeal , 0.001 to 15 fishmeal, 0.001 to 40% soybean flour, 0.001 to 8nm flour , 0.001 to 40% whey powder , 0.1-40 wt% wheat grout, 0.001-30 wt% whey fat mixture, 0.001-5 dicalcium phosphate, 0.001-4 calcium carbonate, 0.001-4 fumaric acid, 0.001-3 per composition 100 g of chlorotetracycline, 100 g of sulfamethazine, as well as a mixture of 50 g of penicillin antibiotic, 0.001 to 1% butter, 0.001 to 1 maple juice concentrate, 0.001 to 1% lysine hydrochloride and 0.001 to 2 copper sulfate, choline, selenium, vitamin E , a vitamin / mineral mixture consisting of biotin, folic acid, glycine and ethoxyquin, and yeast glucan produced from yeasts of Saccharomyces cerevisiae (200-1500 g / tonne). A / S BIOTECMACKZYMAL and CONSOLIDATED A/S BIOTECMACKZYMAL és CONSOLIDATED Instead of NUTRITION LC, the agent:NUTRITION L.C.helyett a meghatalmazott:
Independent claims3
814 paragraphs in 18 sections, as filed
The method of feeding according to the invention is characterized in that the animals are fed with mixtures of feeds which contain enough yeast glucan to increase the growth rate of the animals by at least about 2%.
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• · ·
Representative:
DANUBIA Patent and Trademark Office Ltd.
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minimize potential
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FEED MIXTURES CONTAINING YOUR GLAZED FLUIDS AND ANIMAL FEEDINGSTUFFS BASED ON THEIR APPLICATION
PROCEDURE
Consolidated Nutrition LC, Omaha, USA and A / S BIOTEC-MACKZYMAL, Tromso, Norway
inventors:
POLLMANN D. Steven, Fórt Wayne, USA
HAYEN D. Gary, Bartlesville, USA
Date of filing: 20.07.1994
<td>priority:</td><td>08.08.1993 (102,935), United States States</td>
International Application Number: PCT / US94 / 08152
International Publication Number: WO 95/04467
Our file number is 81271-3030A-tm
Our agent: dr. Palágyi T.
• · • ·
FIELD OF THE INVENTION The present invention relates to compound feed containing yeast glucan, i.e., glucose-only polysaccharides derived from cells of yeast cells, and to a method of feed utilizing the compound feed of the present invention to accelerate the growth of animals.
Given that humanity has only a limited supply of traditional dietary proteins, it is difficult to cope with the rapid growth of the world's population. Of particular importance is the production of animal proteins, which contain the so-called essential amino acids essential for the functioning of the human body. It is a matter of fact that the increase in animal protein production cannot keep pace with the increase in the world population because of limited production capacity and no better production technology.
This is the reason why there is a great need to increase productivity in animal protein production, one of the ways of which is to develop feed mixers that accelerate animal development ·
The present invention has as its object the development of compound feeds, namely compound feeds which accelerate the growth of animals. We also had another goal: to develop a method to increase the growth rate of the animals we feed with the feed compound we are developing. The features and advantages of the invention, as well as further objects which may be achieved by the practice of the invention, will be described in more detail in the following section.
Four embodiments of the invention provide for the preparation of the following compositions or processes:
First Animal growth-promoting compound feed containing at least one starch carrier, at least one protein carrier, at least one fat-containing substance and yeast glucan ·
A method for stimulating animal growth, comprising feeding the animals with compound feed comprising at least one starch carrier, at least one protein carrier, at least one fat-containing material, and yeast glucan.
5th Oral growth stimulating compositions comprising an animal feed and a sufficient amount of yeast glucan to stimulate growth.
4th A method of stimulating the growth of an animal by administering orally compositions of an animal to stimulate the growth of an animal comprising a feed and a yeast glucan.
Group 1 compound feeds can be used to accelerate the development of a wide variety of animals within the scope of the invention, but above all include warm-blooded animals, such as, but not limited to, chickens, pigs, turkeys, and calves. At present, the said compound feed is used mainly in two animals which are just weaned or hatched, such as weaners.
The time needed to stimulate growth can vary widely depending on the type of animal we feed, for example, for weaning pigs, for about 7 to 40 days · · · · · · · · · · · · · · · · ··· · ··· ··· ··· ···
It is preferable to use the compound feeds according to the invention.
Suitable starch carriers are those conventionally used as feed components, generally derived from grain crops such as corn, soybeans, wheat, sorghum, barley and / or oats. Examples of suitable starch carriers include, but are not limited to, corn meal, oatmeal, cornmeal, soybean meal, wheat flour, oatmeal, soybean meal, cornmeal, and the like. Of the suitable starch carriers, oat flour, corn meal, oatmeal, wheat groats, soybean meal and mixtures thereof are the most commonly used. These starch carrier materials are commercially available.
A wide variety of protein carriers can be used as components in the feed mixers of the present invention as long as they are capable of promoting the development of an animal. Protein carriers have a protein content of about 10-90% by weight. For reasons of economy, it is advisable to use proteinaceous raw materials such as fishmeal, dried whey (powdered whey), soy meal or mixtures thereof. In addition, the following protein carriers, which are listed without limitation, are suitable: soy protein concentrate, soybean meal, blood meal, plasma protein, bleached milk powder, whey protein concentrate, cornflour, wheatgrass, Ground and mixtures thereof. Among the appropriate protein carriers are currently fish meal, whey powder, blood meal, plasma protein and ···· · · ·.
We use soy flour most often. These protein carriers are commercially available.
Any fat-containing material capable of promoting the development of an animal can be used in the feed mixers of the present invention. Examples of suitable fat-containing substances include, but are not limited to, lard, tallow, soybean oil, lecithin, coconut fat, whey fat, and mixtures thereof. Soya oil, coconut fat and lard are the most commonly used fatty substances at present.
The term "yeast glucan" as used in the application, unless otherwise indicated, is substantially free of mannose (i.e., glucose, mannose and other hexoses with glycosidic linkages) and phosphomannones or mannoprotins, which is essentially the same as the when you consider your concentration. The backbone of the yeast glucan molecule is mainly composed of β (1-5) -linked glucose units, with a small proportion of intermolecular and extramolecular branches formed by (1-6) linkages. The low-concentration glucan component, which is mainly branched by (1 -6) linkages, is closely associated with the main component. Both components contain glucan-insoluble fractions of alkali.
The glucan content of the yeast glucan useful in the compound feeds of the invention, as measured by the amount of β (1-5) and? (1-6) -linked glucose molecules, is about 40-99 · ♦ · · · · · · · · · · ··· ··: · · .. ·:. :
6% by weight, protein content from about 0.01% to about 50%, lipid content from about 0.01% to about 50%, ash content from about 0.01% to about 12%, and dry matter content from about 10% to about 100%. Preferably, the glucan content is about 40-90 m%, the protein content is about 0.05-50 m%, the lipid content is about 0.05-45, the ash content is about 0.05-10 m% and the dry matter content is about 20-99. more preferably, the glucan content is about 50-90<sup>the</sup> a protein content of about 0.1 to about 10%, a lipid content of about 0.1 to about 40%, an ash content of about 0.5 to about 8 and a solids content of about 70 to about 98%. Most preferably, the glucan content is 60-85 protein
1-8 m%, lipid content 1-55, ash content 1-5 m% and solids content 90-99
The yeast glucan suitable for preparing the compound feeds of the present invention may be derived from any yeast species, but preferably the glucan source is Saccharomyces cerevisiae, Saccharomyces uvarum, Candida utilis, Kluyvecomyces fragilis, Pichia pastoris or a combination thereof. At present, Saccharomyces Cerevisiae or Candida utilis are most often used as a source of yeast glucan, as these yeast species are traditionally used in the edible miscellaneous industry and as feed additives.
Yeast glucan may be prepared from the yeasts referred to in the preceding paragraph by any method known in the art, such as heat treatment, the use of bases, acids, enzymes or solvents, or any combination thereof. Choosing the right method usually depends on which topic experts prefer.
At present, we consider the following process to be useful for the production of yeast glucan:
Suspending the yeast in an alkaline solution (1) to obtain a mixture comprising an insoluble fraction of the yeast cell wall;
(2) separating the insoluble fraction of the yeast cell wall from the mixture;
(5) suspending the insoluble fraction of the yeast cell wall to form a yeast glucan in an acidic solution; and (4) isolating the yeast glucan.
The term "yeast suspension" is used in the application
unless otherwise indicated, refers to a liquid suspension of live yeasts, inactive yeasts, dried yeasts, or any combination thereof.
The yeast glucan useful in the compound feeds of the present invention can be prepared from freshly cultured live yeast culture, a portion of a yeast culture that has already lost some of its viability (i.e., inactive), dried yeast, and any combination thereof. Given that methods for cultivating yeast and producing dried yeast are well known to those of skill in the art, these procedures are omitted to preserve the briefness of the application. The crude yeast glucan may also be prepared by any of the methods known to those skilled in the art, such as by heat treatment, or by treatment with an alkaline, acidic, enzymatic or solvent, or any combination thereof. The choice of the appropriate method for making crude yeast glucan usually depends on the preference of the subject matter expert.
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The yeast suspension mentioned above is generally prepared by adding water to the yeast at a concentration of about 20-200 g / l, preferably about 80-180 g / l, more preferably about 100-160 g / l. Then, in the dissolved state, an alkaline compound is added to the suspension, which is then moved by any suitable means, such as a mechanical stirrer of the mixer, to ensure an even distribution of the alkaline compound in the suspension. Alternatively, the yeast suspension may be added to the alkaline compound.
The alkaline compounds useful in the present invention may be organic bases or inorganic bases in aqueous solution or anhydrous form. The alkaline compound used is usually very soluble in the suspension described in the preceding paragraph. Examples of suitable alkaline compounds include, but are not limited to, tetramethylammonium hydroxide, potassium hydroxide, sodium hydroxide, lithium hydroxide, magnesium hydroxide, calcium hydroxide, lithium carbonate, sodium carbonate, potassium carbonate, magnesium carbonate, and mixtures of two or more of the listed compounds. Sodium hydroxide is the most commonly used alkaline compound as it is easy to obtain and handle.
The amount of alkaline compound required may vary within wide limits, depending on the type of yeast or the crude yeast glucan used. Usually, the above-described suspension is added with enough alkaline compound to provide * · • ·· «
Its concentration in the suspension should be about 0.01 to 6 M, preferably about 0.1 to 5 M, more preferably 0.1 to 2 M.
The mixture thus obtained containing the suspension and the alkaline compound is then heated to a temperature of about 20-120 ° C, preferably about 20-100 ° C. The time required to complete the process is generally about 0.25 to 24 hours, and usually 0.5 to 20 hours. The treatment can be performed over a wide pressure range:
6 4 5 about 10 to 10 Pa, preferably about 5 x 10 and 5 x 10 Pa,
5 more preferably, pressures between 10 and 2 x 10 Pa are used.
After treatment according to the preceding paragraph, an insoluble fraction of the yeast cell wall is formed, which can be separated from the soluble fraction by methods well known to those skilled in the art, such as centrifugation and, if necessary, washing with water.
The insoluble fraction of cell walls is then acidified with an acid. For acidification it is advantageous to use a weak acid such as acetic acid. The insoluble fraction of cell walls is then suspended in water and acid is added to the resulting suspension. Alternatively, said fraction may be directly suspended in an acid. The amount of acid used is sufficient to maintain the cell wall fraction at a pH of about 7 or less. The acidic treatment can be carried out under the same conditions as the alkaline treatment already described. After acid treatment, yeast glucan is prepared.
Yeast glucan may be prepared by any of the methods well known to those skilled in the art, such as centrifugation, filtration, but canterization, or a combination thereof. The recovered yeast glucan can be further washed. Detergents may be water, acetone, methanol, ethanol, aqueous sodium chloride solution, dilute acetic acid, diethyl ether, hexane, or any combination thereof. The recovered yeast glucan may be resuspended in water or dried, depending on various application purposes. Any conventional drying method may be used to dry the yeast glucan product: for example, spray drying, rotary drying, freeze drying, air drying, or any combination thereof.
The feed compositions of the present invention may also contain water-soluble vitamins as well as trace elements. Suitable vitamins include, but are not limited to, vitamin A, vitamin D, vitamin E, vitamin K, riboflavin, stric acid, niacin, vitamin B4, folic acid, biotin, vitamin C, and any mixture thereof. Suitable trace elements include, but are not limited to, copper, zinc, iodine, selenium, manganese, iron, cobalt, and any combination of these elements, and may be mentioned compounds of these elements. The word "trace" in the word "trace element" refers to the amount of elements used in the compound feed, which is much lower than the other components. These vitamins and trace elements are generally present in an amount of about 0.0001-5%, based on the total weight of the compound feed.
The feed compositions of the present invention may also contain antioxidant additives such as ethoxycin, BHT, BHA, vitamin E, ascorbic acid, and mixtures of these components.
• 4»*· ·* '<sup>w</sup> · · ♦ · 'I • · · 6 · · * ·: ···: ·· .. ♦ :. :
The feed compositions of the present invention may also contain antibiotics: for example, chlorotetracycline, glutamethazine, penicillin and mixtures of these antibiotics.
Antioxidants and antibiotics are generally present in very small amounts, for example about 0.0001 to 1% by weight, in the feed compositions of the invention.
The starch carrier material may be present in the feed mixture in any concentration, with the restriction that it must effectively promote the growth of the animals. The starch carrier material in the feed compositions of the invention is usually present in an amount of from about 10 to about 80% by weight, preferably from about 15 to about 50% by weight, more preferably from about 20 to about 40%.
The protein carrier may be present in the feed mixture in any concentration effective to promote the growth of the animals. The protein carrier in the compound feed compositions of the present invention is generally present in an amount of about 10-50 µm, preferably about 15-40 µm, more preferably about 18-50 µm.
As with the previous components, the fat-containing material may be present in the feed compositions of the present invention at any concentration that facilitates the growth of the animals. The fat-containing materials in the feed compositions of the invention are usually present in an amount of from about 2% to about 20%, preferably from about 4% to about 15%, more preferably from about 6% to about 12%.
The amount of yeast glucan present in the compound feed according to the invention should be such that the growth rate of the animals is at least about 2, preferably 4% and more preferably 6%. According to the invention,
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• · · ·
Feed mixtures 12 generally contain from about 0.001% to about 10% by weight of yeast glucan, preferably from about 0.01% to about 5% by weight, and more preferably from about 0.02% to about 2% by weight. The mixture of vitamins and minerals already described is present in the feed compositions of the present invention in an amount of about 0.0001-5 n%, preferably about 0.001-5 m%, more preferably 0.01-2 m%.
All percentages given are based on the total weight of the compound feed at 100%.
An example of a preferred compound feed composition of the present invention is the following with reference to the quantitative range of each component:
<td>- ground oatmeal</td><td> 0,001-50</td><td>m%</td>
<td>- oatmeal</td><td> 0,001-50</td><td>m%</td>
<td>- cornmeal</td><td> 0,001-70</td><td>m%</td>
<td>- fishmeal</td><td> 0,001-15</td><td>m%</td>
<td>- soy meal</td><td> 0,001-40</td><td>m%</td>
<td>- soybean meal</td><td> 0,001-8</td><td>m%</td>
<td>- whey powder</td><td> 0,001-40</td><td>m%</td>
<td>- semolina</td><td> 0,001-50</td><td>m%</td>
<td>- whey fat</td><td> 0,001-50</td><td>m%</td>
<td>- dicalcium phosphate</td><td> 0,001-5</td><td>m%</td>
<td>- calcium carbonate</td><td> 0,001-4</td><td>m%</td>
<td>- fumaric acid</td><td> 0,001-4</td><td>m%</td>
<td>- be on antibiotics</td><td> 0,001-5</td><td>m%</td>
<td>- butter</td><td> 0,001-1</td><td>m%</td>
<td>- sheep's juice</td><td> 0,001-1</td><td>m%</td>
<td>- lysine hydrochloride</td><td> 0,001-1</td><td>m%</td>
<td>- vitamin / mineral mixture</td><td> 0,001-2</td><td>m%</td>
Again, the percentages are based on the total weight of the compound feed according to the invention, corresponding to 100 n $. The antibiotic fly contains 100 g of chlorotetracycline, 100 g of sulfamethazine and 50 g of penicillin per liter of feed mixture. The vitamin / mineral mixture contains copper sulfide, choline, selenium, vitamin E, biotin, folic acid, glycine and ethoxyquin.
Further examples of the composition of the compound feeds of the present invention are provided in the feed examples.
Feed mixtures according to Embodiment 1 of the present invention may be prepared by any mixing method known to those skilled in the art, such as mechanical mixing, extrusion, pelletization and spray drying. The nutritional value and physical properties of the feed are usually independent of the order in which the individual components are mixed. However, in this connection it is noted that it is preferable to first mix the yeast glucan with a carrier such as rice kernels, maize bran, wheat bran, calcium carbonate or a mixture thereof to facilitate uniform distribution during blending.
The compound feeds of the present invention may be used in any form, such as powder, pelletized, cube-shaped, semi-solid or any combination thereof.
An animal growth stimulating method of Embodiment 2 of the present invention comprises feeding the animals with a feed composition of the present invention which has a composition of Embodiment 1 which is a composition of Embodiment 1 of the present invention. · · · · · ······ · ····· ·
14 and are prepared by methods. Methods of feeding animals with compound feeds are well known to those of ordinary skill in the art, and therefore, for the sake of completeness, the disclosure of specific feeding methods is omitted. The choice of specific feeding methods will generally depend on the particular method preferred by the practitioner.
Compositions of Embodiment 3 may be used to stimulate growth of animals by oral administration. All animals that respond to the compositions of the invention will exhibit a growth rate of at least about 2%, preferably about 4%, more preferably about 6%, when fed with any of said compositions. Such animals are listed, for example, in the description of embodiment 1 of the invention.
According to Embodiment 3 of the present invention, any animal feed commercially available or prepared by those skilled in the art may be used. For example, suitable animal feeds usually include a carbohydrate, a protein, and a lipid. These materials may be the same as those described in Figure 1 of the present invention. and are also used in the percentages given therein. Suitable animal feeds may also contain water and fat soluble vitamins, as well as trace elements and antioxidant additives, which may be the same as those described in the embodiment of the present invention and may also be used in the percentages specified therein * • * · · · 9 9 9 ···· • · * · ««.
• · · · · · • ····· · · ·
The same definition as used in connection with embodiment 1 of the invention applies to the yeast glucan used in connection with embodiment 5.
The yeast glucan should be used in the composition in such concentration or amount that it is sufficient to increase the rate of development of the animals fed with the compositions by at least about 2%, preferably about 4%, more preferably about 6%. About 50-2500 g, preferably about 100-2000 g, more preferably 200-1500 g / yeast glucan are used per tonne of the composition. The amount of yeast glucan required to achieve the above mentioned developmental rates can also be expressed in units of weight per animal per day. For weaning piglets, for example, the daily requirement of yeast glucan is, for example, about 100-450 mg, preferably about 150-400 mg, more preferably 200-550 mg.
Compositions according to Embodiment 5 may be prepared in the same manner as compositions according to Embodiment 1.
The method of Embodiment 4 of the present invention can be used to stimulate the rate of development of an animal by feeding the animals with compositions containing an animal feed and yeast glucan. The compositions as well as the animals fed may be the same as those described in connection with the embodiment of the invention.
The following specific examples are provided to further illustrate the invention. The examples are not intended to serve as a basis for any unlawful limitation of the invention in any way.
• « ·
<img file="HUT70567A_D0009.tif" />
Example I
This example is a general description of how to make a yeast glucan that can be used in the compound feeds and compositions of the present invention.
500 g of dry Saccharomyces cerevisiae was suspended in 3 L of 6% aqueous sodium hydroxide. The resulting slurry was stirred overnight at ambient temperature and centrifuged at 2000 g for 25 minutes. The supernatant was removed and the insoluble residue was resuspended in 5 L of 5% sodium hydroxide solution. The resulting suspension was incubated at 75 ° C for 5 hours and then cooled overnight. Subsequently, the suspension was centrifuged at 2000 g for 25 minutes and the supernatant was decanted.
The residue containing the insoluble material was adjusted to pH 4.5 with acetic acid and the insoluble residue was washed three times with 2 liters of water. After each wash, the insoluble residue was recovered by centrifuging the aqueous slurry for 25 minutes at 2000 rpm and discarding the supernatant. The residue was then suspended in 5 L of 0.5 M aqueous acetic acid. The resulting suspension was kept at 90 ° C for 3 hours and then cooled to ambient temperature. The insoluble residue was recovered by centrifugation for 25 minutes at 2000 rpm.
The insoluble residue was then suspended in 3 L of distilled water and the resulting slurry was stirred at 100 ° C for 3θ minutes. After centrifugation, the supernatant was discarded, and the insoluble residue was spray-dried and the dried material was used to carry out the procedures described in the following examples.
II. example
The following example illustrates the effect of yeast glucose with bonds (1-3) and (1-6) on the growth of weaning piglets.
A randomized full block experiment was designed in which piglets were fed with the compositions of the invention. The yeast glucan in the Example, prepared as described in Example I, contained approximately 60% glucan, approximately 6 uf protein, approximately 14% lipid and typhoid moisture as measured by glucose units, and had an ash content of approximately 9%. .
I and II. at 6 different concentrations, i.e. 0.0 per tonne; 0.227; 0,454; 0.681; 0.908 and 1.135 kg of yeast glucan were used. (Of these, 0.0 kg represents the amount of yeast glucan used in the control diet.) Four or five 19-21 day-old weaning piglets were housed in a cage and fed in a pre-planned manner. (In the following, feeding with a defined amount and composition of feed is referred to as dietary feeding.) Each yeast diet was fed with 6 different amounts of yeast glucan. The composition of the diet was determined according to current feed standards (Table I), which promote rapid animal development.
··· ·
- 18 Feeding weaning pigs were fed with the feed composition for the first period from day 0 to day 15. This feed, based on 1.25 m% assimilable lysine and containing 5500 kcal / kg of energy, contained 16 m% whey powder, 5 assorted menhaden fish meal, 10 oat flour and 10 m% oatmeal,
For the second period, feed was selected between 14 days and 55 days, which is the last day of the experiment, with feed composition. This feed, based on 1.15 assimilable lysine and containing 5200 kcal / kg of energy, contained 6% whey powder, 5 assorted menhaden fishmeal and 10% oatmeal,
Feeding results were determined using the least squares method. The linear, quadratic and cubic differences were examined by statistical modeling. The piglets were weighed 7 and 15 days after the start of the experiment to determine more precisely the effect of the amounts of yeast glucan added during growth phase I,
The composition of the diets is given in Table I ·
Table I: Dietary feed for choice piglets
<td>Identification of the components</td><td>HSP 121-126<sup>the</sup>. m%</td><td>HSP 127-152%</td>
<td>corn flour</td><td> 27,86</td><td> 45,10</td>
<td>Oatmeal</td><td> 10,00</td><td> 10,00</td>
<td>Oatmeal</td><td> 10,00</td><td> -</td>
<td>Whey meal</td><td> 11,55</td><td> 6,00</td>
* ··· · ······ * · · · · • · · · ···· · ·· • ··«·
- Table 19 (continued)
<td>Identification of the components</td><td>HSP 121-126 *,% m</td><td>HSP 127-152<sup>b</sup>.</td>
<td>soy flour</td><td> 21,87</td><td> 21,99</td>
<td>Made of assorted quality menhaden</td><td></td><td></td>
<td>fish meal</td><td> 5,00</td><td> 5,00</td>
<td>7/40 whey / fat mixture</td><td> 7,79</td><td> -</td>
<td>soybean pod</td><td> 1,68</td><td> -</td>
<td>V--</td><td> -</td><td> 10,00</td>
<td>Good quality white fat</td><td> -</td><td> 2,55</td>
<td>Dicalcium phosphate (18.5%)</td><td> 0,85</td><td> 1,08</td>
<td>Calcium carbonate</td><td> 0,65</td><td> 0,72</td>
<td>Salt</td><td> -</td><td> 0,19</td>
<td>Grass price acid</td><td> 1,25</td><td> -</td>
<td>L-Li zin--hid.ro ki orid</td><td> 0,11</td><td> 0,19</td>
<td>ASP-250 premix<sup>0</sup></td><td> 0,50</td><td> 0,50</td>
<td>CS-butter</td><td> 0,50</td><td> 0,20</td>
<td>Corn dextrose</td><td> 0,25</td><td> 0,25</td>
<td>θ Other components</td><td> 0,60</td><td> 0,45</td>
<td>Calculated amount of nutrients</td><td></td><td></td>
<td>crude protein</td><td> 21,00</td><td> 19,85</td>
<td>grease</td><td> 6,00</td><td> 5,51</td>
<td>fiber</td><td> 2,47</td><td> 2,96</td>
<td>Calcium</td><td> 0,88</td><td> 0,80</td>
<td>Phosphorus</td><td> 0,70</td><td> 0,70</td>
<td>Energy content, kcal / kg</td><td> 5500</td><td> 5200</td>
<td>Built-in amino acids</td><td></td><td></td>
<td>- lysine</td><td> 1,25</td><td> 1,15</td>
<td>- methionine</td><td> 0,57</td><td> 0,54</td>
<td>- threonine</td><td> 0,78</td><td> 0,69</td>
<td>- tryptophan</td><td> 0,25</td><td> 0,25</td>
• · · · · · · · · · · · · · · · · · · · · · · · · · · ···
- 20 <sup>the</sup>HSP 121-126: dietary feed used in the first phase of the experiment · ^ HSP 127-152: dietary feed used in the second phase of the experiment.
<sup>C</sup>ASP 25O premix: Provides 100 grams of chlorotetracycline, 100 grams of sulfamethazine and 5θ grams of penicillin per tonne of feed · Corn Dextrose 0.025? 0,050? It was replaced by θ, 10075 »0.100 and 0.125 glucan if it contained yeast glucan among the components of the diet.
®Small ingredients present in small amounts, in the form of premixtures of vitamins and trace elements commonly used in pigs. The premix contains the following ingredients: copper sulfate, choline, selenium, vitamin E, biotin, folic acid, glycine and / or ethoxyquin.
The effect of yeast glucan is shown in Table II. are shown in Table. During the first seven days of phase I of the experiment, the increase in yeast glucan concentration resulted in a quadratic increase in food intake (P <0.05). Piglets consumed more dietary feed while the yeast glucan concentration of the feed increased from 0 kg / t to 0.453 kg / t, but further increase in concentration had little effect on nutrient uptake. The daily growth of piglets developed in the same way as food intake. Adding more yeast glucans to the Stage I diet during the last six days of the first period of the experiment results in a linear decrease in daily gain (P <0.02) and daily food intake (P <0.05). II. Table 3 shows optimum growth and food intake when the piglets were fed with
··· · • · · · · · • ····· · · · ··· · ·· ·· ·
- Contains 0.227 kg and 0.455 kg of yeast glucan per 21 tonnes, respectively.
II. It also appears from the data in Table 3 that weaners fed with feed containing 0.227 kg of yeast glucan increased 14.3% faster and made 8.7% better use of feed than non-yeast glucan fed animals.
It can also be seen in the II. Based on the results in Table II, daily weight gain (P <0.07) and daily diet (P <0.05) increased in a square manner over the last 20 days of the experiment, as the yeast glucan content of the diet increased. In the final phase of the experiment, optimum weight gain and food intake were determined for piglets fed with feed containing 0.227 kg of yeast glucan per tonne. For the entire duration of the experiment, it was found that with the increase in the amount of yeast glucan added to the feed, the weight gain of the piglets was squared. II. It can be seen from Table 1 to 4 that the piglets' growth performance was highest when fed with the lowest feed containing yeast glucan at a concentration of 0.227 kg / t. Thus, as the yeast glucan content of the feed increases, this power is reduced. Thus, the results of this experiment show that yeast glucan accelerates the development of animals and improves food intake when used in low concentrations in feed.
- 22 II. Effect of yeast glucose levels added to feed on growth of weaning pigs
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- 25 II. Table (continued)
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<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>the</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>CO</td>
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<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>the</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td> 0</td>
<td>K</td><td></td><td></td><td></td><td></td><td>X</td><td>K</td><td>LfA</td><td>ex</td><td></td><td></td><td>IX</td><td>X</td><td>CM</td><td>X</td><td>r4</td>
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<td>4b</td><td></td><td>X</td><td></td><td></td><td>4b</td><td>4b</td><td>4b</td><td>4b</td><td></td><td></td><td>4b</td><td>4b</td><td>4b</td><td> ·»</td><td>P</td>
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<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td> 94</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>t4 a</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>4-r Φ</td>
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<td>cu</td><td></td><td>ua</td><td></td><td></td><td>4-r</td><td>K</td><td>X</td><td>UA</td><td></td><td></td><td></td><td>CM</td><td></td><td>K</td><td>Λ1</td>
<td>4b</td><td></td><td>K</td><td></td><td></td><td> *</td><td>4b</td><td>4b</td><td>4b</td><td></td><td></td><td>4b</td><td> *</td><td>4b</td><td> *</td><td>CD</td>
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<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>P</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>Φ</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>Ü</td>
<td></td><td></td><td></td><td></td><td></td><td>cu</td><td>K</td><td>ex</td><td>LfA</td><td></td><td></td><td>SHE</td><td>ex</td><td> 00</td><td>ex</td><td>4b</td>
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<td>4b</td><td></td><td></td><td></td><td></td><td> 4»</td><td>4b</td><td>4b</td><td>4b</td><td></td><td></td><td>4b</td><td> ·»</td><td>4b</td><td></td><td>Φ</td>
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<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td> •14</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>tq</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>CD</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td> 1</td><td></td><td></td><td></td><td></td><td></td><td>ND</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td colspan="2"> 94</td><td></td><td> 0</td><td></td><td></td><td colspan="2">Ό</td><td></td><td>ü</td>
<td></td><td></td><td></td><td></td><td></td><td></td><td>needle</td><td>P</td><td></td><td>qr</td><td></td><td colspan="2">T3</td><td>P</td><td></td><td></td>
<td></td><td></td><td></td><td> 1</td><td></td><td> 94</td><td>P</td><td>P</td><td>Φ</td><td>CO</td><td colspan="2">'SHE</td><td>P</td><td>P</td><td></td><td>r4</td>
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<td></td><td></td><td></td><td> ></td><td></td><td>P</td><td>:She</td><td>tq</td><td>'CD</td><td>CO</td><td></td><td>P</td><td>:She</td><td>tq</td><td>WOMAN</td><td>P</td>
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<td>g</td><td></td><td></td><td>Φ</td><td></td><td>tq</td><td>:She</td><td>Λ1</td><td>SHE</td><td>needle</td><td></td><td>N</td><td>:She</td><td>ü</td><td> 0</td><td>φ</td>
<td>'CD</td><td></td><td></td><td> 94</td><td></td><td>:She</td><td>ü</td><td></td><td>CD</td><td></td><td></td><td>:She</td><td></td><td></td><td>CD</td><td>CO</td>
<td>-ld</td><td></td><td> ·.</td><td> 3</td><td></td><td>Λ4</td><td></td><td>ft</td><td></td><td>CO</td><td></td><td>P</td><td></td><td>Ά</td><td></td><td> •14</td>
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<td> 50</td><td></td><td>-Φ</td><td>4-r</td><td></td><td>the</td><td></td><td> •</td><td>P</td><td></td><td></td><td>Ű</td><td></td><td> •</td><td>P</td><td>Φ</td>
<td>P</td><td></td><td> ></td><td>Λ</td><td></td><td></td><td> •</td><td>K</td><td>Ό</td><td>4-r</td><td></td><td></td><td> •</td><td>K</td><td>Ό</td><td>P</td>
<td>N</td><td></td><td>r-I</td><td>'CO</td><td></td><td> •</td><td>SO</td><td>SO</td><td>CD</td><td>Φ</td><td></td><td> •</td><td>SO</td><td>K</td><td>CD</td><td>Φ</td>
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<td colspan="2">Φ CÖ</td><td> 94</td><td></td><td></td><td></td><td></td><td>CO</td><td>jd</td><td>Ό</td><td></td><td></td><td></td><td>CO</td><td></td><td></td>
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<td>Ό</td><td> \</td><td>g</td><td>Ά</td><td></td><td></td><td></td><td>CD</td><td>N</td><td>1 l</td><td>ai</td><td></td><td></td><td>CD</td><td>tq</td><td>Φ</td>
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<td> >3</td><td>λϊ</td><td></td><td>the</td><td>CO</td><td>ό</td><td>-Φ</td><td></td><td>'Φ</td><td>Ή</td><td>SHE</td><td>'Φ</td><td>'Φ</td><td></td><td>-Φ</td><td> >></td>
<td>g</td><td></td><td></td><td></td><td>Ű</td><td></td><td></td><td> •</td><td>needle</td><td> 3</td><td>Ό</td><td></td><td></td><td> •</td><td>DD</td><td>the</td>
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<td></td><td>CO</td><td>i-4 00</td><td></td><td>Φ</td><td></td><td></td><td></td><td></td><td></td><td>'CO</td><td></td><td></td><td></td><td></td><td>co</td>
<td> <4</td><td>P</td><td>Ή S</td><td></td><td>-P</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td>CO</td>
Yeast glucan uptake was calculated by multiplying the concentration of yeast glucan in the feed in kg / t by the total feed intake.
•·<sup>3</sup>» .·· • · · · · · • ···· · · · · ♦·· · ·· ·· ·
- 24 <sup>c</sup>Linear glucan effect (P <0.02). Glucan effect (P <0.07) · Glucan effect (P <0.01). Squared glucan effect (P <0.05). ^ Linear glucan effect (P <0.05) ·
III. example
The effect of adding yeast glucan to the feed was also tested in six additional randomized whole block experiments as described above. The same feed mixtures of the composition described in Table I were used with 0.05 µl sto-glucan in art litter which were mixed with the feed mixtures in Table II. The pigs were fed in stages 1 and 2 of the experiment of Example 1-3. In III. The results reported in Table I show that when yeast glucan is added to the feed, the weaning piglets develop significantly better (P <0.05). The average of the results from the six studies shows that yeast glucan increased the daily growth of piglets by 10.5%, and at the end of the experiment the average weight of the piglets fed the yeast glucan feed was 1.5 kg higher than the piglets not receiving yeast glucan. Particularly interesting was the experience that - in II. In accordance with the results of Table 1, there was no difference in the efficiency of feed utilization for weight gain. This suggests that the rapid development of piglets is due solely to the presence of yeast glucan in the compound feed.
- 25 III. Table 1: Effects of yeast glucan on the development of weaning piglets
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···· 4
- 26 ει<sup>y</sup>
Animals fed with feed containing no yeast glucan.
<sup>b</sup>Animals fed with feed containing 0.05% w / w yeast glucan.
ARC. example
This example demonstrates that the acceleration in the development of the weaning piglets fed with yeast glucan is not due to the antibacterial or antifungal activity of the yeast glucan.
The assay was performed in the same manner as in Example II. Example IV, antibiotics IV. except when leaving table.
ARC. Table 1: Effect of drug-containing feed additive on yeast glucan on development of weaning piglets
<td>Feed yeast glucan-</td><td> 0</td><td> 0,10</td><td> 0,10</td>
<td>content, m%</td><td></td><td></td><td></td>
<td>Pharmacy litter<sup>D</sup></td><td> +</td><td> -</td><td> +</td>
<td>Piglets are the weight of the test</td><td> 18,42</td><td> 17,85</td><td> 19,25</td>
<td>end, kg<sup>G</sup></td><td></td><td></td><td></td>
<td>Daily gain, kg / day</td><td> 0,54</td><td> 0,55</td><td> 0,57</td>
<td>Daily feed intake, kg / day<sup>C</sup></td><td> 0,48</td><td> 0,46</td><td> 0,52</td>
<td>Daily feed intake and</td><td> 1,59</td><td> 1,40</td><td> 1,58</td>
<td>daily growth rate</td><td></td><td></td><td></td>
Six cages of piglets per treatment were included in the experiment.
The drug was 110 ppm sulfamethazine, 110 ppm chlorotetracycline and 55 PP<sup>m</sup> penicillin was added to the weight of the feed between trains.
<sup>c</sup>P <0.05.
... J · ♦ ·· ♦ »· • · · ···· * · • ti {· ·«
- 27 A IV. Table 1 shows that the weight gain of piglets fed with non-drug feed slightly decreased from 0.34 kg / day to 0.33 kg / day, indicating that the drug content plays an important role in anti-bacterial or antifungal activity. . In the case of feed mixes containing both yeast glucan and drug, the weight gain increased significantly - from 0.34 kg / day to 0.37 kg / day - indicating that yeast glucan increased animal development rate. The IV. The data in Table II also justify - Table II. and III. Like the data in Example 1 - that yeast glucan does not improve feed utilization.
Example V
This comparative example is provided to illustrate that feed mixtures containing autolysed yeast or intact yeast cells do not accelerate the development of weaning piglets.
The experiments were carried out as described in Example I, but with the formulations of formulas V and VI in the feed compositions of Table I. Yeast products according to Table II are added.
The data in Table V demonstrate that yeast cell walls or yeast cells are not capable of accelerating the developmental rate of animals. We do not intend to delve into theoretical considerations, just to note that, in our opinion, the upper part of the digestive tract of animals, i.e., the stomach and small intestine, is not capable of releasing protein, fat, and other components from yeast cell walls or cells. thus, it cannot have a beneficial effect on animals.
<img file="HUT70567A_D0015.tif" />
Table II: Autumn comparisons of yeast glucan and alternative yeast products as feed additives
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<img file="HUT70567A_D0016.tif" />
VI. Table 1: Effect of the addition of feed compound with yeasts on the development of weaning piglets
<img file="HUT70567A_D0017.tif" />
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II. Section 7.7 5.5 14.9 -10.4 -5.0 -5.5 for the entire duration of the experiment 6.5 6.5 11.9 -11.8 -15.5 -4.8
- 50 • · ·
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- 31 As stated in Section VI. Table 1 shows that daily weight gain, daily food intake and nutritional efficiency were reduced when autolysed yeast or intact yeast cells were added to the feed mixture. It can also be stated that daily gain and daily food intake were lower when II. In Section III, the piglets were fed with autolysed yeast or intact yeast cells, although a feed with 5.0 autolysed yeast or intact yeast cells achieved better results than 2.5% autolysed yeast, which is a higher nutritional value of the additive. In addition, the provisions of Annex VI. The data in Table I also show that, compared to control data, yeast glucan increases the daily gain and the utilization rate of the feed supplemented with it.
It is clear from the results presented in the examples of the specification that the invention can be effectively used to achieve the objects mentioned at the beginning of the specification. By using the invention, the advantages described above as well as the implementation of the invention can be provided. Those skilled in the art can modify the disclosed solutions, but such modifications are within the scope of the invention as defined in the specification and claims.
Contents18
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25 members in 13 offices
Priority claims1
| Document | Office | Kind | Date |
|---|---|---|---|
| 10293593 | United States of America | A |
Members25
| Document | Office | Kind | |
|---|---|---|---|
| CA2145858A1 | Canada | A1 | |
| WO9504467A1 | World Intellectual Property Organization (WIPO) | A1 | |
| FI951615A | Finland | A | |
| NO951316D0 | Norway | D0 | |
| NO951316L | Norway | L | |
| HU9500996D0 | Hungary | D0 | |
| PL308293A1 | Poland | A1 | |
| EP0664671A1 | European Patent Office (EPO) | A1 | |
| EP0664671A4 | European Patent Office (EPO) | A4 | |
| HUT70567AThis record | Hungary | A | |
| JPH08504600A | Japan | A | |
| PL177628B1 | Poland | B1 | |
| NO313172B1 | Norway | B1 | |
| EP0664671B1 | European Patent Office (EPO) | B1 | |
| AT223655T | Austria | T | |
| ATE223655T1 | Austria | T1 | |
| DE69431342D1 | Germany | D1 | |
| DK0664671T3 | Denmark | T3 | |
| PT664671E | Portugal | E | |
| ES2182845T3 | Spain | T3 | |
| DE69431342T2 | Germany | T2 | |
| CA2145858C | Canada | C | |
| JP2008029361A | Japan | A | |
| JP4074659B2 | Japan | B2 | |
| FI120859B | Finland | B |
1 legal event, as the office reported them to INPADOC
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Numbers
- Application
- 9500996
Titles
- English
- ANIMAL FEEDS COMPRISING YEAST GLUCAN AND FEED PROCESS FOR GROWTH OF ANIMALS BASED ON FEEDS COMPRISING YEAST GLUCAN
Classification
- CPC, 3
- A23K50/60
- A23K10/12
- A23K20/163
- IPC, 4
- A23K1 00
- A23K1 16
- A23K1 18
- A23L1 28