Quantification of polynucleotide analytes from dried samples

There is described a method of quantifying a polynucleotide analyte present in a bodily fluid sample that dried to produce a dried bodily fluid sample, the method comprising the steps of: (a) performing a nucleic acid amplification reaction using the dried bodily fluid sample as a source of templates to produce amplification products and obtain a measured result, the measured result indicating a concentration or an amount of the polynucleotide analyte; and (b) multiplying the measured result by a correction factor to obtain a corrected result, wherein the correction factor is the solution to an equation that specifies the correction factor as a function of the measured result, and wherein the equation comprises a non-linear equation, thereby quantifying the polynucleotide analyte present in the bodily fluid sample.