Small molecule trail gene induction by normal and tumor cells as an anticancer therapy

FIELD OF THE INVENTION

0001The present invention relates generally to compositions for use in treating cancer.

BACKGROUND OF THE INVENTION

0002TNF-related apoptosis-inducing ligand (TRAIL; Apo2L) is an endogenous protein that selectively induces apoptosis in cancer cells.

0003TRAIL is a powerful inducer of apoptosis in a wide range of human cancer cell lines via pro-apoptotic death receptor 4 (DR4; TRAIL-R1) and death receptor 5 (DR5; TRAII,-R2) at the cell surface through engagement of the extrinsic or intrinsic apoptotic pathways. TRAIL plays a direct role in tumor suppression during immune surveillance but this anti-tumor mechanism is lost during the disease progression. The ability of TRAIL to initiate apoptosis selectively in cancer cells has led to ongoing clinical trials with administration of recombinant TRAIL and the longer-lived TRAIL-agonist antibodies targeting either of its two pro-apoptotic death receptors.

0004Despite its potency, recombinant TRAIL has efficacy-limiting properties such as short serum half-life, stability, cost, and delivery. Delivery of recombinant TRAIL or TRAIL-agonist antibodies to the brain is limited by inability of recombinant TRAIL and TRAIL-agonist antibodies to cross the blood-brain barrier.

0005<nplcit id="ncit0001" npl-type="s"><text>Allen et al., (2011) Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; Apr 2-6, 2011; Orlando, FL. Cancer Res; 71(8 Suppl): Abstract 4502. doi:10.1158/1538-7445.AM2011-4502</text></nplcit>, discloses that the small molecule TIC10 has potent anticancer efficacy mediated by induction of TRAIL production in normal and tumor cells.

0006<nplcit id="ncit0002" npl-type="s"><text> Allen et al., (2012) Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; March 31-Apr 4, 2012; Chicago, IL. Cancer Res. 72(8), Abstract 1935, XP055125782</text></nplcit>, discloses that the potent anti-tumor effects of TIC10 require Foxo3a and TRAIL gene upregulation.

0007DTP data warehouse index results for NSC350625, and in particular the US National Institute developmental therapeutics program dose response curves for NSC350625, discloses in vitro results for cell lines incubated with NSC350625.

0008There is a continuing need for anti-cancer compositions and methods.

SUMMARY OF THE INVENTION

0009The present invention provides a pharmaceutical composition, comprising the compound NSC350625, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, for use in a method of treating CNS cancer, non-small cell lung cancer or lymphoma in a subject. In an embodiment, the method is a treatment of a CNS cancer selected from the group consisting of gliomas, meningiomas, pituitary adenomas, nerve sheath tumors, and oligodendrogliomas

0010In an embodiment, the method further comprises administering a second therapeutic agent to the subject, optionally the second therapeutic agent comprises an anti-cancer agent. In an embodiment, the anti-cancer agent is a mitotic inhibitor. In an embodiment, the anti-cancer agent is paclitaxel, docetaxel or a combination thereof.

0011In an embodiment, the method further comprises administering a second therapeutic agent to the subject, wherein the second therapeutic agent comprises an anti-angiogenic agent. In an embodiment, the anti-angiogenic agent is bevacizumab.

0012In an embodiment, the composition is administered orally. In other embodiments, the composition is administered via rectal, nasal, pulmonary, epidural, ocular, otic, intraarterial, intracardiac, intracerebroventricular, intradermal, intravenous, intramuscular, intraperitoneal, intraosseous, intrathecal, intravesical, subcutaneous, topical, transdermal, transmucosal, sublingual, buccal, vaginal or inhalational routes of administration.

0013In an embodiment, the method further comprises assessing effectiveness of the treatment, wherein assessing effectiveness of the treatment comprises assaying TNF-related apoptosis-inducing ligand in a biological sample obtained from the subject. In an embodiment, the biological sample is blood, serum, plasma or cerebrospinal fluid.

BRIEF DESCRIPTION OF THE DRAWINGS

0014<ul id="ul0001" list-style="none" compact="compact"><li><figref idref="f0001">Figure 1</figref> is a graph showing activity of luciferase reporter in HCT116 Bax^-/- cells under transcriptional control of the first 504 base pairs of the human TRAIL gene promoter upstream of the start of transcription;</li><li><figref idref="f0001">Figure 2</figref> is a graph showing RT-qPCR analysis of TRAIL mRNA levels in HCT116 p53^-/- cells;</li><li><figref idref="f0002">Figure 3</figref> is a graph showing surface TRAIL levels induced by NSC350625 in a panel of cancer cells;</li><li><figref idref="f0002">Figure 4</figref> is a graph showing surface TRAIL levels in HCT116 p53^-/- cells following NSC350625 treatment at indicated conditions and time points;</li><li><figref idref="f0002">Figure 5</figref> is a graph showing HCT116 p53^-/- TRAIL surface levels by flow cytometry at 72 hr following NSC350625 treatment initiation;</li><li><figref idref="f0003">Figure 6</figref> shows cell cycle profiles of HCT116 p53^-/- and human foreskin fibroblast (HFF) cells treated with NSC350625;</li><li><figref idref="f0003">Figure 7</figref> is a graph showing quantification of colony formation assays of cancer cells treated with NSC350625;</li><li><figref idref="f0004">Figure 8</figref> is a graph showing parallel experiments as in <figref idref="f0003">Figure 7</figref> but with HFF cells that were enumerated at endpoint;</li><li><figref idref="f0005">Figure 9</figref> is a graph showing sub-G1 analysis of HCT116 WT, p53^-/-, and Bax^-/- cells following treatment with DMSO, NSC350625 or rhTRAIL (25 ng/mL);</li><li><figref idref="f0005">Figure 10</figref> is an image showing Western blot analysis results;</li><li><figref idref="f0005">Figure 11</figref> is a graph showing sub-G1 analysis of NSC350625-treated cancer cells pre-incubated with or without zVAD-fmk;</li><li><figref idref="f0006">Figure 12</figref> is a graph showing sub-G1 analysis of MDA-MB-231 cells with stable knockdown of TRAIL by short hairpin RNA;</li><li><figref idref="f0006">Figure 13</figref> is a graph showing verification of MDA-MB-231 shTRAII, knockdown by flow cytometry analysis of NSC350625-treated cells;</li><li><figref idref="f0006">Figure 14</figref> is a graph showing sub-G1 analysis of NSC350625-induced cell death in H460 cells with endogenous DR5 or overexpression of a DR5 construct with its death domain replaced by EGFP;</li><li><figref idref="f0007">Figure 15</figref> is a graph showing sub-G1 analysis of HCT116 cells treated with DMSO, NSC350625, or rhTRAIL in the presence or absence of a TRAIL-sequestering antibody, RIK-2;</li><li><figref idref="f0007">Figure 16</figref> is a graph showing NSC350625-induced surface TRAIL with freshly resected human colon cancer cells;</li><li><figref idref="f0007">Figure 17</figref> is a graph showing results of a cell viability assay of primary colon cancer cells from <figref idref="f0007">Figure 16</figref> treated with DMSO, NSC350625 or 5-FU;</li><li><figref idref="f0008">Figure 18</figref> is a graph showing ability of NSC350625 or rhTRAIL to reduce cell viability in HCT116 cells following a 1 hr pre-incubation at the indicated temperatures;</li><li><figref idref="f0008">Figure 19</figref> is a graph showing HCT116 p53^-/- xenograft treated with NSC350625, TRAIL, or vehicle;</li><li><figref idref="f0008">Figure 20</figref> is a graph showing results of bioluminescent imaging of luciferase-infected HCT116 p53^-/- xenografts treated with NSC350625 or vehicle;</li><li><figref idref="f0009">Figure 21</figref> is a graph showing RKO xenograft treated with NSC350625, TRAIL or vehicle;</li><li><figref idref="f0009">Figure 22</figref> is a box and whisker plot of tumor volume on day 9 following treatment initiation in MDA-MB-231 vector or shTRAIL xenografts with NSC350625, TRAIL or vehicle;</li><li><figref idref="f0009">Figure 23</figref> is a graph showing relative tumor volume of DLD-1 xenografts treated with TRAIL, NSC350625 or DMSO;</li><li><figref idref="f0010">Figure 24</figref> is a graph showing comparison of i.p. versus oral administration of NSC350625 in SW480 xenografts;</li><li><figref idref="f0010">Figure 25</figref> is a graph showing NSC350625 or vehicle administered as a single oral dose in the HCT116 xenograft;</li><li><figref idref="f0010">Figure 26</figref> is a graph showing body weight of athymic, female nude mice treated with a single dose of NSC350625;</li><li><figref idref="f0011">Figure 27</figref> is a graph showing body weight of C57B6 female mice at the end of week 4 of treatment with oral NSC350625;</li><li><figref idref="f0011">Figure 28</figref> is a graph showing overall survival of Eµ-myc treated during weeks 9-12 with weekly oral NSC350625;</li><li><figref idref="f0011">Figure 29</figref> is a graph showing cell viability of DLD-1 cells treated with NSC350625 in combination with paclitaxel;</li><li><figref idref="f0012">Figure 30</figref> is a graph showing cell viability of SW620 cells treated with NSC350625 in combination with paclitaxel;</li><li><figref idref="f0012">Figure 31</figref> is a graph showing cell viability of DLD-1 cells treated with NSC350625 in combination with taxotere;</li><li><figref idref="f0012">Figure 32</figref> is a graph showing cell viability of SW620 cells treated with NSC350625 in combination with taxotere;</li><li><figref idref="f0013">Figure 33</figref> is a graph showing percent of cohorts in H460 xenograft that retain tumor burden following treatment with NSC350625 or taxotere alone, in combination, or with vehicle;</li><li><figref idref="f0013">Figure 34</figref> is a graph showing a relative tumor volume plot for <figref idref="f0013">Figure 33</figref>;</li><li><figref idref="f0013">Figure 35</figref> is a graph showing percent of cohorts in H460 xenograft that retain tumor burden following treatment with NSC350625 or paclitaxel alone, in combination, or with vehicle;</li><li><figref idref="f0014">Figure 36</figref> is a graph showing a relative tumor volume plot for <figref idref="f0013">Figure 35</figref>;</li><li><figref idref="f0014">Figure 37</figref> is a graph showing percent of cohorts with implanted with intracecal HCT116 p53^-/- tumors with evident tumors at the primary and distal sites at endpoint, treated with NSC350625, bevacizumab or a combination of NSC350625 and bevacizumab;</li><li><figref idref="f0015">Figure 38</figref> is a graph showing body weight of mice implanted with intracecal HCT116 p53^-/- tumors treated with vehicle, NSC350625, bevacizumab or a combination of NSC350625 and bevacizumab;</li><li><figref idref="f0015">Figure 39</figref> is a graph showing TRAIL serum levels in tumor-free mice following NSC350625 or doxorubicin;</li><li><figref idref="f0015">Figure 40</figref> is a graph showing the absorbance profile of NSC350625 with a peak absorbance at 239nm;</li><li><figref idref="f0016">Figure 41</figref> is a graph showing a calibration curve for NSC350625 spiked into mouse plasma and quantitated by HPLC analysis using area under curve (AUC);</li><li><figref idref="f0016">Figure 42</figref> is a graph showing plasma concentrations of NSC350625 following intravenous administration in C57B6 female mice;</li><li><figref idref="f0016">Figure 43</figref> is a graph showing surface TRAIL analysis of HFF cells following NSC350625 treatment, 0, 2.5, 5, or 10 µM from left to right;</li><li><figref idref="f0016">Figure 44</figref> is a graph showing sub-G1 analysis of a co-culture of HCT116 p53^-/- cells and pretreated HFFs;</li><li><figref idref="f0017">Figure 45</figref> is a graph showing surface TRAIL in GBM cell lines following incubation with NSC350625;</li><li><figref idref="f0017">Figure 46</figref> is a graph showing GI50 values extrapolated from cell viability assays of indicated GBM cell lines at 72 hr post-treatment with NSC350625 or DMSO;</li><li><figref idref="f0017">Figure 47</figref> shows results of a cell viability assay of freshly resected human glioblastoma tissue treated with DMSO, NSC350625, or temozolomide;</li><li><figref idref="f0018">Figure 48</figref> is a graph showing subcutaneous xenograft of T98G with mice receiving a single dose of vehicle, NSC350625 or bevacizumab;</li><li><figref idref="f0018">Figure 49</figref> is a graph showing overall survival of mice harboring SF767 intracranial tumors treated with a single oral dose of vehicle, NSC350625, bevacizumab, or NSC350625 and bevacizumab;</li><li><figref idref="f0019">Figure 50</figref> is a graph showing transcriptional changes associated with FOXO signaling from gene expression profiling of HCT116 p53^-/- cells at 48 hr post-NSC350625 treatment versus DMSO;</li><li><figref idref="f0019">Figure 51</figref> is an image of Western blot analysis of DR5 in HCT116 cells treated with NSC350625 or DMSO;</li><li><figref idref="f0019">Figure 52</figref> is a graph showing flow cytometry analysis of surface DR5 levels in cancer and normal cells treated with NSC350625;</li><li><figref idref="f0020">Figure 53</figref> is an image of Western blot analysis of whole cell lysates (W) and cytoplasmic (C) and nuclear (N) extracts from HCT116 cells treated with DMSO or NSC350625;</li><li><figref idref="f0020">Figure 54</figref> is an image of results of a chromatin immunoprecipitation assay for NSC350625-induced translocation of Foxo3a to the TRAIL promoter at 48 hr post-NSC350625 treatment in HCT116 p53^-/- cells, 0, 2.5, 5, or 10 µM from left to right;</li><li><figref idref="f0020">Figure 55</figref> is a graph showing results of flow cytometry analysis of cell surface TRAIL levels induced by NSC350625 with or without transient knockdown of Foxo1 and/or Foxo3a in HCT116 p53^-/- cells using siRNA;</li><li><figref idref="f0020">Figure 56</figref> is a graph showing sub-G1 analysis of NSC350625-induced cell death with or without stable knockdown of Foxo3a in HCT116 cells;</li><li><figref idref="f0021">Figure 57</figref> is a graph showing flow cytometry analysis of NSC350625-induced surface TRAIL with or without stable knockdown of Foxo3a in HCT116 cells;</li><li><figref idref="f0021">Figure 58</figref> is a graph showing tumor volume of HCT116 xenograft with or without stable knockdown of Foxo3a following a single oral dose of vehicle or NSC350625;</li><li><figref idref="f0022">Figure 59</figref> is an image of Western blot analysis of HCT116 p53^-/- cells treated with NSC350625, 2.5, 5, 10 µM for 72 hr;</li><li><figref idref="f0022">Figure 60</figref> is an image of Western blot analysis of HCT116 p53^-/- cells treated with NSC350625;</li><li><figref idref="f0023">Figure 61</figref> is a graph showing time course of protein expression levels of NSC350625-induced effects determined by densitometry of Western blots from replicate experiments as in <figref idref="f0022">Figure 60</figref>;</li><li><figref idref="f0023">Figure 62</figref> is an image of Western blot analysis of NSC350625-induced effects on Foxo3a in DLD1 human colon cancer cells, MDA-MB-468 human breast cancer cells, and T98G human glioblastoma multiforme cell lines;</li><li><figref idref="f0023">Figure 63</figref> is an image of Western blot analysis showing overexpression of myr-Akt;</li><li><figref idref="f0024">Figure 64</figref> is a graph showing flow cytometry analysis of surface TRAIL in HCT116 cells overexpressing an empty vector or myristilated Akt (myr-Akt) with NSC350625 treatment;</li><li><figref idref="f0024">Figure 65</figref> is a graph showing sub-G1 content of HCT116 cells overexpressing an empty vector or myr-Akt with NSC350625 treatment;</li><li><figref idref="f0025">Figure 66</figref> is a graph showing RT-qPCR analysis of TRAIL mRNA in HCT116 p53^-/- cells following incubation with A6730 (Akt inh), U0126 monoethanolate (MEK inh), or both;</li><li><figref idref="f0025">Figure 67</figref> is a graph showing surface TRAIL induction as in <figref idref="f0025">Figure 66</figref> with or without stable knockdown of Foxo3a;</li><li><figref idref="f0026">Figure 68</figref> is a graph showing sub-G1 analysis of MDA-MB-231 with or without TRAIL knockdown by shRNA following incubation with Akt inh, MEK inh, or both;</li><li><figref idref="f0026">Figure 69</figref> is a graph showing surface TRAIL analysis of HCT116 p53^-/- cells following incubation with A6730 (Akt inh), U0126 monoethanolate (MEK inh), or both;</li><li><figref idref="f0027">Figure 70</figref> is a graph showing RT-qPCR analysis of TRAIL mRNA levels following transient knockdown of Akt and/or ERK in HCT116 p53^-/- cells;</li><li><figref idref="f0027">Figure 71</figref> is an image showing confirmation of Akt and ERK knockdown by Western blot analysis; and</li><li><figref idref="f0027">Figure 72</figref> is a graph showing surface TRAIL analysis following transient knockdown of Akt and/or ERK in HCT116 cells.</li></ul>

DETAILED DESCRIPTION OF THE INVENTION

0015Scientific and technical terms used herein are intended to have the meanings commonly understood by those of ordinary skill in the art. Such terms are found defined and used in context in various standard references illustratively including <nplcit id="ncit0003" npl-type="b"><text>J. Sambrook and D.W. Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press; 3rd Ed., 2001</text></nplcit>; <nplcit id="ncit0004" npl-type="b"><text>F.M. Ausubel, Ed., Short Protocols in Molecular Biology, Current Protocols; 5th Ed., 2002</text></nplcit>;<nplcit id="ncit0005" npl-type="b"><text> B. Alberts et al., Molecular Biology of the Cell, 4th Ed., Garland, 2002</text></nplcit>; <nplcit id="ncit0006" npl-type="b"><text>D.L. Nelson and M.M. Cox, Lehninger Principles of Biochemistry, 4th Ed., W.H. Freeman &amp; Company, 2004</text></nplcit>; <nplcit id="ncit0007" npl-type="b"><text>Engelke, D.R., RNA Interference (RNAi): Nuts and Bolts of RNAi Technology, DNA Press LLC, Eagleville, PA, 2003</text></nplcit>; <nplcit id="ncit0008" npl-type="b"><text>Herdewijn, P. (Ed.), Oligonucleotide Synthesis: Methods and Applications, Methods in Molecular Biology, Humana Press, 2004</text></nplcit>; <nplcit id="ncit0009" npl-type="b"><text>A. Nagy, M. Gertsenstein, K. Vintersten, R. Behringer, Manipulating the Mouse Embryo: A Laboratory Manual, 3rd edition, Cold Spring Harbor Laboratory Press; December 15, 2002, ISBN-10: 0879695919</text></nplcit>; <nplcit id="ncit0010" npl-type="b"><text>Kursad Turksen (Ed.), Embryonic stem cells: methods and protocols in Methods Mol Biol. 2002;185, Humana Press; Current Protocols in Stem Cell Biology, ISBN: 9780470151808</text></nplcit>.

0016The singular terms "a," "an," and "the" are not intended to be limiting and include plural referents unless explicitly state or the context clearly indicates otherwise.

0017Compositions according to aspects of the present invention relate to TRAIL-inducing compound 10 (NSC350625), identified by the present inventors as a small molecule transcriptional inducer of the TRAIL gene by a screen for TRAIL-inducing compounds that upregulate the TRAIL gene by a mechanism that does not rely on p53 since p53 is frequently inactivated in late stage cancers, which causes resistance to many standard-of-care therapies such as 5-FU and doxorubicin.

0018NSC350625 induces TRAIL expression in both normal and cancer cells. The terms "induces TRAIL expression," "NSC350625-induced TRAIL" and grammatical equivalents thereof, used herein to describe an effect of NSC350625 or a pharmaceutically acceptable salt thereof, refers to production of a detectable increase of TRAIL by cells contacted with NSC350625 or a pharmaceutically acceptable salt thereof. A detectable increase of TRAIL can be determined by assays for TRAIL protein or TRAIL nucleic acids using well-known protein or nucleic acid assay methodology.

0019NSC350625-induced TRAIL is sustained in cancer cells as well as normal cells and serum, allowing for a TRAIL-mediated bystander effect on cancer cells and tumors. NSC350625 inactivates Akt and ERK leading to the nuclear translocation of Foxo3a and induction of TRAIL transcription.

0020NSC350625-induced TRAIL is dependent on Foxo3a, which also upregulates TRAIL death receptor DR5 among other targets, allowing for sensitization of some TRAIL-resistant tumor cells. The induction of TRAIL caused by NSC350625 is sustained in tumor, stromal, and host cells.

0021The present invention thus provides a pharmaceutical composition comprising the compound NSC350625, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, for use in a method of treating CNS cancer, non-small cell lung cancer or lymphoma in a subject.

0022The compound NSC350625 can be obtained commercially or synthesized using standard chemical synthetic methodology.

0023Pharmaceutically acceptable salts of NSC350625 can be obtained commercially or synthesized using standard chemical synthetic methodology.

0024A "pharmaceutically acceptable" salt is suitable for use in a subject without undue toxicity or irritation to the subject and is effective for the intended use.

0025Pharmaceutically acceptable salts include pharmaceutically acceptable acid addition salts and base addition salts. Pharmaceutically acceptable salts are well-known in the art, such as those detailed in <nplcit id="ncit0011" npl-type="s"><text>S. M. Berge et al., J. Pharm. Sci., 66: 1-19, 1977</text></nplcit>. Exemplary pharmaceutically acceptable salts are those suitable for use in a subject without undue toxicity or irritation to the subject and which are effective for their intended use which are formed with inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, nitric acid, phosphoric acid, sulfuric acid and sulfamic acid; organic acids such as acetic acid, adipic acid, alginic acid, ascorbic acid, aspartic acid, benzenesulfonic acid, benzoic acid, 2-acetoxybenzoic acid, butyric acid, camphoric acid, camphorsulfonic acid, cinnamic acid, citric acid, digluconic acid, ethanesulfonic acid, formic acid, fumaric acid, glutamic acid, glycolic acid, glycerophosphoric acid, hemisulfic acid, heptanoic acid, hexanoic acid, 2-hydroxyethanesulfonic acid (isethionic acid), lactic acid, maleic acid, hydroxymaleic acid, malic acid, malonic acid, mandelic acid, mesitylenesulfonic acid, methanesulfonic acid, naphthalenesulfonic acid, nicotinic acid, 2-naphthalenesulfonic acid, oxalic acid, pamoic acid, pectinic acid, phenylacetic acid, 3- phenylpropionic acid, picric acid, pivalic acid, propionic acid, pyruvic acid, pyruvic acid, salicylic acid, stearic acid, succinic acid, sulfanilic acid, tartaric acid, p-toluenesulfonic acid, trichloroacetic acid, trifluoroacetic acid and undecanoic acid; inorganic bases such as ammonia, hydroxide, carbonate, and bicarbonate of ammonium; organic bases such as primary, secondary, tertiary and quaternary amine compounds ammonium, arginine, betaine, choline, caffeine, diolamine, diethylamine, diethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, dicyclohexylamine, dibenzylamine, N, N-dibenzylphenethylamine, 1-ephenamine, N, N'-dibenzylethylenediamine, ethanolamine, ethylamine, ethylenediamine, glucosamine, histidine, hydrabamine, isopropylamine, 1h-imidazole, lysine, methylamine, N-ethylpiperidine, N-methylpiperidine, N-methylmorpholine, N, N-dimethylaniline, piperazine, trolamine, methylglucamine, purines, piperidine, pyridine, theobromine, tetramethylammonium compounds, tetraethylammonium compounds, trimethylamine, triethylamine, tripropylamine and tributylamine and metal cations such as aluminum, calcium, copper, iron, lithium, magnesium, manganese, potassium, sodium, and zinc.

0026Pharmaceutical compositions for use in the invention may include NSC350625 or a pharmaceutically acceptable salt thereof as a first therapeutic agent; a pharmaceutically acceptable carrier; and a second therapeutic agent, such as an anti-cancer agent.

0027TRAIL protein can be assayed in a test sample obtained from a subject to detect NSC350625-induced TRAIL expression.

0028Immunoassay methods can be used to assay TRAIL in a sample, including enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunofiltration assay (ELIFA), flow cytometry, immunoblot, immunoprecipitation, immunohistochemistry, immunocytochemistry, luminescent immunoassay (LIA), fluorescent immunoassay (FIA), and radioimmunoassay. Assay methods may be used to obtain qualitative and/or quantitative results. Specific details of suitable assay methods for both qualitative and quantitative assay of a sample are described in standard references, illustratively including <nplcit id="ncit0012" npl-type="b"><text>E.Harlow and D. Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1988</text></nplcit>; <nplcit id="ncit0013" npl-type="b"><text>F. Breitling and S. Dübel, Recombinant Antibodies, John Wiley &amp; Sons, New York, 1999</text></nplcit>; <nplcit id="ncit0014" npl-type="b"><text>H. Zola, Monoclonal Antibodies: Preparation and Use of Monoclonal Antibodies and Engineered Antibody Derivatives, Basics: From Background to Bench, BIOS Scientific Publishers, 2000</text></nplcit>; <nplcit id="ncit0015" npl-type="b"><text>B.K.C. Lo, Antibody Engineering: Methods and Protocols, Methods in Molecular Biology, Humana Press, 2003</text></nplcit>; <nplcit id="ncit0016" npl-type="b"><text>F. M. Ausubel et al., Eds., Short Protocols in Molecular Biology, Current Protocols, Wiley, 2002</text></nplcit>; <nplcit id="ncit0017" npl-type="b"><text>S. Klussman, Ed., The Aptamer Handbook: Functional Oligonucleotides and Their Applications, Wiley, 2006</text></nplcit>;<nplcit id="ncit0018" npl-type="b"><text> Ormerod, M. G., Flow Cytometry: a practical approach, Oxford University Press, 2000</text></nplcit>; <nplcit id="ncit0019" npl-type="b"><text>Givan, A. L., Flow Cytometry: first principles, Wiley, New York, 2001</text></nplcit>; <nplcit id="ncit0020" npl-type="b"><text>Gorczyca, W., Flow Cytometry in Neoplastic Hematology: morphologic-immunophenotypic correlation, Taylor &amp; Francis, 2006</text></nplcit>; <nplcit id="ncit0021" npl-type="b"><text>Crowther, J. R., The ELISA Guidebook (Methods in Molecular Biology), Humana Press, 2000</text></nplcit><nplcit id="ncit0022" npl-type="b"><text>; Wild, D., The Immunoassay Handbook, 3rd Edition, Elsevier Science, 2005</text></nplcit>; and<nplcit id="ncit0023" npl-type="b"><text> J. Sambrook and D.W. Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 3rd Ed., 2001</text></nplcit>.

0029Aptamers can be used to assay a sample for TRAIL. The term "aptamer" refers to a peptide and/or nucleic acid that substantially specifically binds to a specified substance. In the case of a nucleic acid aptamer, the aptamer is characterized by binding interaction with a target other than Watson/Crick base pairing or triple helix binding with a second and/or third nucleic acid. Such binding interaction may include Van der Waals interaction, hydrophobic interaction, hydrogen bonding and/or electrostatic interactions, for example. Similarly, peptide-based aptamers are characterized by specific binding to a target wherein the aptamer is not a naturally occurring ligand for the target. Techniques for identification and generation of peptide and nucleic acid aptamers and their use are known in the art as described, for example, in <nplcit id="ncit0024" npl-type="b"><text>F. M. Ausubel et al., Eds., Short Protocols in Molecular Biology, Current Protocols, Wiley, 2002</text></nplcit>; <nplcit id="ncit0025" npl-type="b"><text>S. Klussman, Ed., The Aptamer Handbook: Functional Oligonucleotides and Their Applications, Wiley, 2006</text></nplcit>; and <nplcit id="ncit0026" npl-type="b"><text>J. Sambrook and D.W. Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 3rd Ed., 2001</text></nplcit>.

0030Spectrometric analysis is used to assay a sample for TRAIL. For example mass analysis can be used in an assay according to aspects of the present invention. Mass analysis is conducted using, for example, time-of-flight (TOF) mass spectrometry or Fourier transform ion cyclotron resonance mass spectrometry. Mass spectrometry techniques are known in the art and exemplary detailed descriptions of methods for protein and/or peptide assay are found in <nplcit id="ncit0027" npl-type="s"><text>Li J., et al., Clin Chem., 48(8): 1296-304, 2002</text></nplcit>; <nplcit id="ncit0028" npl-type="s"><text>Hortin, G.L., Clinical Chemistry 52: 1223-1237, 2006</text></nplcit>;<nplcit id="ncit0029" npl-type="s"><text> Hortin, G.L., Clinical Chemistry 52: 1223-1237, 2006</text></nplcit>; <nplcit id="ncit0030" npl-type="b"><text>A.L. Burlingame, et al. (Eds.), Mass Spectrometry in Biology and Medicine, Humana Press, 2000</text></nplcit>; and<nplcit id="ncit0031" npl-type="b"><text> D.M. Desiderio, Mass Spectrometry of Peptides, CRC Press, 1990</text></nplcit>.

0031Localization of TRAIL at the surface of cells can be assayed to detect an effect of a pharmaceutical composition of the present invention. Detection of TRAIL localization can be performed by immunoassay, such as flow cytometry, as well as by immunohistochemistry.

0032A test sample can be any biological fluid, cell or tissue of a subject, illustratively including blood, plasma, serum, urine, saliva, ascites, cerebrospinal fluid, cerebroventricular fluid, pleural fluids, pulmonary and bronchial lavage samples, mucous, sweat, tears, semen, bladder wash samples, amniotic fluid, lymph, peritoneal fluid, synovial fluid, bone marrow aspirate, tumor cells or tissue, organ cells or tissue, such as biopsy material. In preferred aspects, a test sample is blood, plasma or serum.

0033A test sample from a subject is optionally purified for TRAIL or other biomarker assay. The term "purified" in the context of a test sample refers to separation of TRAIL or another biomarker from at least one other component present in the test sample. Test sample purification is achieved by techniques illustratively including electrophoretic methods such as gel electrophoresis and 2-D gel electrophoresis; chromatography methods such as HPLC, ion exchange chromatography, affinity chromatography, size exclusion chromatography, thin layer and paper chromatography.

0034Assay of TRAIL can be performed on cells and tissues. For example, immunohistochemical methods and in situ hybridization can be used to assay TRAIL protein and/or nucleic acid in a cell or tissue test sample.

0035One or more standards can be used to allow quantitative determination of TRAIL in a sample.

0036Assay of TRAIL in a test sample may be compared to assay of TRAIL in a control sample. Control samples may be obtained from one or more normal subjects, for example.

0037According to aspects of the present invention, assays for TRAIL are used to monitor a subject. Thus, for example, a test sample is obtained from the subject before treatment with a pharmaceutical composition of the present invention and at one or more times during and/or following treatment in order to assess effectiveness of the treatment. In a further example, a test sample is obtained from the subject at various times in order to assess the course or progress of disease or healing.

0038In particular aspects, one or more additional biomarkers are assayed in a test sample obtained from a subject to aid in monitoring treatment with a pharmaceutical composition of the present invention. For example, one or more of phospho-ERK, phospho-Akt, Foxo3a localization and/or phosphorylation is assayed in a test sample obtained from a subject to aid in monitoring treatment with a pharmaceutical composition of the present invention. Such additional biomarkers are assayed by immunoassay methods such those described herein.

0039TRAIL nucleic acid can be assayed in a test sample obtained from a subject to detect NSC350625-induced TRAIL expression. Assays for detecting TRAIL nucleic acids, particularly mRNA or cDNA, include polymerase chain reactions (PCR) such as RT-PCR, dot blot, in situ hybridization, Northern blot and RNase protection.

0040It is disclosed herein that treatment of a subject having, or at risk of having, cancer, by administration of a pharmaceutically effective amount of NSC350625 or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier; is effective to induce expression of TRAIL in the subject.

0041CNS cancer, non-small cell lung cancer or lymphoma treated using compositions described herein are characterized by abnormal cell proliferation including pre-neoplastic hyperproliferation, cancer in-situ, neoplasms and metastasis. Compositions of the present invention can be used for prophylaxis as well as amelioration of signs and/or symptoms of cancer. The terms "treating" and "treatment" used to refer to treatment of a cancer in a subject include: preventing, inhibiting or ameliorating the cancer in the subject, such as slowing progression of the cancer and/or reducing or ameliorating a sign or symptom of the cancer.

0042A pharmaceutically effective amount of a composition for use in the present invention is an amount which has a beneficial effect in a subject being treated. In subjects having CNS cancer, non-small cell lung cancer or lymphoma cancer, a pharmaceutically effective amount of a composition of the present invention is effective to ameliorate or prevent one or more signs and/or symptoms of the condition, or is effective to detectably increase apoptosis and/or decrease proliferation of CNS cancer, non-small cell lung cancer or lymphoma cells.

0043NSC350625 possesses broad-spectrum activity described herein in primary patient samples and cell lines that are resistant to conventional therapies, indicative that the therapeutic action of NSC350625 does not rely exclusively on commonly altered molecules in cancer such as EGFR, Her2, KRAS, or PTEN. The elucidation of the therapeutic cellular mechanism of NSC350625 allows for the identification of resistance mechanisms such as over-activated Akt, described herein, and provides phospho-ERK, phospho-Akt, Foxo3a localization and phosphorylation, and surface and serum TRAIL as correlative biomarkers of NSC350625 therapeutic activity in cancer.

0044Thus, according to aspects of the present invention, one or more correlative biomarkers of NSC350625 therapeutic activity in cancer may be assayed to assess treatment with the pharmaceutical composition.

0045A subject treated using compositions of the present invention can be mammalian or non-mammalian. A mammalian subject can be any mammal including a human; a non-human primate; a rodent such as a mouse, rat, or guinea pig; a domesticated pet such as a cat or dog; a horse, cow, pig, sheep, goat, or rabbit. A non-mammalian subject can be any non-mammal including a duck, goose, chicken, or turkey. Subjects can be either gender and can be any age. In aspects of the invention, the subject is human. The terms "subject" and "patient" are used interchangeably herein.

0046A pharmaceutical composition for use according to the invention generally includes about 0.1-99% of NSC350625, a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier. Combinations of NSC350625 and at least one pharmaceutically acceptable salt thereof in a pharmaceutical composition are also considered within the scope of the present invention.

0047Combinations of therapeutic agents may be administered according to aspects of the present invention. According to aspects of the present invention, treatment of CNS cancer, non-small cell lung cancer or lymphoma in a subject may include administration of a pharmaceutical composition of NSC350625, a pharmaceutically acceptable salt thereof; and at least one additional therapeutic agent. According to aspects of the present invention, the treatment may include administration of a pharmaceutical composition of NSC350625, a pharmaceutically acceptable salt thereof; and at least two additional therapeutic agents.

0048The term "additional therapeutic agent" is used herein to refer to a chemical compound, a mixture of chemical compounds, a biological macromolecule (such as a nucleic acid, an antibody, a protein or portion thereof, e.g., a peptide), or an extract made from biological materials such as bacteria, plants, fungi, or animal (particularly mammalian) cells or tissues which is a biologically, physiologically, or pharmacologically active substance (or substances) that acts locally or systemically in a subject.

0049Additional therapeutic agents may include antibiotics, antivirals, antineoplastic agents, analgesics, antipyretics, antidepressants, antipsychotics, anti-cancer agents, antihistamines, anti-osteoporosis agents, anti-osteonecrosis agents, antiinflammatory agents, anxiolytics, chemotherapeutic agents, diuretics, growth factors, hormones, non-steroidal antiinflammatory agents, steroids and vasoactive agents.

0050Combination therapies utilizing NSC350625, or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents may show synergistic effects, e.g., a greater therapeutic effect than would be observed using a pharmaceutical composition of the present invention including NSC350625, or a pharmaceutically acceptable salt thereof, or one or more additional therapeutic agents alone as a monotherapy.

0051According to aspects, combination therapies include: (1) pharmaceutical compositions that include a pharmaceutical composition including NSC350625, or a pharmaceutically acceptable salt thereof formulated together in a single composition with one or more additional therapeutic agents; and (2) co-administration of a pharmaceutical composition including NSC350625, or a pharmaceutically acceptable salt thereof, with one or more additional therapeutic agents wherein the pharmaceutical composition including NSC350625 or a pharmaceutically acceptable salt thereof and the one or more additional therapeutic agents have not been formulated in the same composition. When using separate formulations, the pharmaceutical composition including NSC350625 or pharmaceutically acceptable salt thereof may be administered at the same time, intermittent times, staggered times, prior to, subsequent to, or combinations thereof, with reference to the administration of the one or more additional therapeutic agents.

0052Combination treatments can allow for reduced effective dosage and increased therapeutic index of the pharmaceutical composition including NSC350625, a pharmaceutically acceptable salt thereof of the present invention and the one or more additional therapeutic agents used in methods of the present invention.

0053According to aspects, combination therapies include: (1) pharmaceutical compositions that include a pharmaceutical composition including NSC350625, or a pharmaceutically acceptable salt thereof formulated together in a single composition with one or more additional anti-cancer agents; and (2) co-administration of a pharmaceutical composition including NSC350625, or a pharmaceutically acceptable salt thereof with one or more additional anti-cancer agents wherein the pharmaceutical composition including NSC350625, or pharmaceutically acceptable salt thereof and the one or more additional therapeutic agents have not been formulated in the same composition. When using separate formulations, the pharmaceutical composition including NSC350625, or pharmaceutically acceptable salt thereof may be administered at the same time, intermittent times, staggered times, prior to, subsequent to, or combinations thereof, with reference to the administration of the one or more additional anti-cancer agents.

0054Anti-cancer agents are described, for example, in<nplcit id="ncit0032" npl-type="b"><text> Goodman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th Ed., Macmillan Publishing Co., 1990</text></nplcit>.

0055Anti-cancer agents illustratively include acivicin, aclarubicin, acodazole, acronine, adozelesin, aldesleukin, alitretinoin, allopurinol, altretamine, ambomycin, ametantrone, amifostine, aminoglutethimide, amsacrine, anastrozole, anthramycin, arsenic trioxide, asparaginase, asperlin, azacitidine, azetepa, azotomycin, batimastat, benzodepa, bevacizumab, bicalutamide, bisantrene, bisnafide dimesylate, bizelesin, bleomycin, brequinar, bropirimine, busulfan, cactinomycin, calusterone, capecitabine, caracemide, carbetimer, carboplatin, carmustine, carubicin, carzelesin, cedefingol, celecoxib, chlorambucil, cirolemycin, cisplatin, cladribine, crisnatol mesylate, cyclophosphamide, cytarabine, dacarbazine, dactinomycin, daunorubicin, decitabine, dexormaplatin, dezaguanine, dezaguanine mesylate, diaziquone, docetaxel, doxorubicin, droloxifene, dromostanolone, duazomycin, edatrexate, eflomithine, elsamitrucin, enloplatin, enpromate, epipropidine, epirubicin, erbulozole, esorubicin, estramustine, etanidazole, etoposide, etoprine, fadrozole, fazarabine, fenretinide, floxuridine, fludarabine, fluorouracil, flurocitabine, fosquidone, fostriecin, fulvestrant, gemcitabine, hydroxyurea, idarubicin, ifosfamide, ilmofosine, interleukin II (IL-2, including recombinant interleukin II or rIL2), interferon alfa-2a, interferon alfa-2b, interferon alfa-n1, interferon alfa-n3, interferon beta-Ia, interferon gamma-Ib, iproplatin, irinotecan, lanreotide, letrozole, leuprolide, liarozole, lometrexol, lomustine, losoxantrone, masoprocol, maytansine, mechlorethamine hydrochlride, megestrol, melengestrol acetate, melphalan, menogaril, mercaptopurine, methotrexate, metoprine, meturedepa, mitindomide, mitocarcin, mitocromin, mitogillin, mitomalcin, mitomycin, mitosper, mitotane, mitoxantrone, mycophenolic acid, nelarabine, nocodazole, nogalamycin, ormnaplatin, oxisuran, paclitaxel, pegaspargase, peliomycin, pentamustine, peplomycin, perfosfamide, pipobroman, piposulfan, piroxantrone hydrochloride, plicamycin, plomestane, porfimer, porfiromycin, prednimustine, procarbazine, puromycin, pyrazofurin, riboprine, rogletimide, safingol, semustine, simtrazene, sparfosate, sparsomycin, spirogermanium, spiromustine, spiroplatin, streptonigrin, streptozocin, sulofenur, talisomycin, tamoxifen, tecogalan, tegafur, teloxantrone, temoporfin, teniposide, teroxirone, testolactone, thiamiprine, thioguanine, thiotepa, tiazofurin, tirapazamine, topotecan, toremifene, trestolone, triciribine, trimetrexate, triptorelin, tubulozole, uracil mustard, uredepa, vapreotide, verteporfin, vinblastine, vincristine sulfate, vindesine, vinepidine, vinglycinate, vinleurosine, vinorelbine, vinrosidine, vinzolidine, vorozole, zeniplatin, zinostatin, zoledronate, and zorubicin.

0056Synergistic effects of combination treatment with a pharmaceutical composition including NSC350625 with one or more additional anti-cancer agents such as one or more mitotic inhibitors and/or one or more anti-angiogenic agents is unexpectedly found as described herein.

0057According to aspects of the present invention, treating a subject having CNS cancer, non-small cell lung cancer or lymphoma includes administration of a therapeutically effective amount of NSC350625, or a pharmaceutically acceptable salt thereof; and a mitotic inhibitor.

0058According to aspects of the present invention, treating a subject having CNS cancer, non-small cell lung cancer or lymphoma includes administration of a therapeutically effective amount of NSC350625, or a pharmaceutically acceptable salt thereof; and a taxane mitotic inhibitor, such as paclitaxel and docetaxel.

0059According to aspects of the present invention, treating a subject having CNS cancer, non-small cell lung cancer or lymphoma includes administration of a therapeutically effective amount of NSC350625, or a pharmaceutically acceptable salt thereof; and an anti-angiogenic agent.

0060According to aspects of the present invention, treating a subject having CNS cancer, non-small cell lung cancer or lymphoma includes administration of a therapeutically effective amount of NSC350625, or a pharmaceutically acceptable salt thereof; and an anti-angiogenic agent, such as bevacizumab.

0061In particular aspects of inventive compositions, the amount of the adjunct anti-cancer agent administered is less than an amount of the adjunct anti-cancer agent necessary to achieve a therapeutic effect if administered without administration of a therapeutically effective amount of NSC350625 or pharmaceutically acceptable salt thereof. Thus, in particular aspects of compositions for use in the present invention, the amount of the adjunct anti-cancer agent in a unit dose of the composition is at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, or at least 90%, less than an amount of the adjunct anti-cancer agent necessary to achieve a therapeutic effect if administered without the therapeutically effective amount of NSC350625 or pharmaceutically acceptable salt thereof.

0062It is also disclosed that TRAIL can be induced or provided by other methods or compositions to administration of NSC350625, such as by administration of one or more histone deacetylase (HDAC) inhibitors such as vorinostat, described in <nplcit id="ncit0033" npl-type="s"><text>Nebbioso, A. et al, 2005, Nat Med 11, 77-84</text></nplcit>; one or more TRAIL-agonist antibodies such as lexatumumab and mapatumumab; and/or recombinant TRAIL such as adenoviral TRAIL as described in <nplcit id="ncit0034" npl-type="s"><text>Abdulghani, J. et al., 2010, Exp. Opin. Ther. Targets 14:1091-1108</text></nplcit>.

0063Optionally, treating a subject having CNS cancer, non-small cell lung cancer or lymphoma further includes an adjunct anti-cancer treatment. An adjunct anti-cancer treatment can be a radiation treatment of a subject or an affected area of a subject's body.

0064TRAIL expression induced in the subject by administration of a pharmaceutical composition in accordance with the present invention is detectable in a sample obtained from the subject, such as a blood sample obtained from the subject.

0065Aspects of the present invention may result in upregulation of the TRAIL gene by normal and tumor tissues with sustained serum levels of secreted TRAIL, after a single dose of NSC350625, for 3-4 days. Normally the serum half-life of the TRAIL protein is 20-30 minutes.

0066NSC350625 has a calculated mass of 387.21 and crosses the blood-brain barrier. Administration of NSC350625 allows for induction of TRAIL in cells of the central nervous system, illustratively including glial cells and neurons of the brain and spinal cord. Further, administration of a pharmaceutically acceptable derivative, salt, ester, amide, hydrate, solvate or prodrug of NSC350625 which crosses the blood-brain barrier allows for induction of TRAIL in cells of the central nervous system.

0067The present invention includes compositions for use in methods of treating a subject having a central nervous system (CNS) cancer by a route of administration other than by direct administration to the CNS.

0068Primary CNS cancers and CNS metastases of non-CNS cancers, also called brain cancer herein, are treated according to aspects of the present invention. Primary CNS cancers treated according to aspects of the present invention include gliomas, meningiomas, pituitary adenomas and nerve sheath tumors. Glioblastoma multiforme is a primary CNS cancer treated according to aspects of the present invention. Oligodendrogliomas are primary CNS cancers treated according to aspects of the present invention.

0069Administration of a pharmaceutical composition for use in the present invention may be by a route of administration including oral, rectal, nasal, pulmonary, epidural, ocular, otic, intraarterial, intracardiac, intracerebroventricular, intradermal, intravenous, intramuscular, intraperitoneal, intraosseous, intrathecal, intravesical, subcutaneous, topical, transdermal, and transmucosal, such as by sublingual, buccal, vaginal, and inhalational, routes of administration.

0070A pharmaceutical composition for use in the present invention may be in any dosage form suitable for administration to a subject, including solid, semi-solid and liquid dosage forms such as tablets, capsules, powders, granules, suppositories, pills, solutions, suspensions, ointments, lotions, creams, gels, pastes, sprays and aerosols. Liposomes and emulsions are well-known types of pharmaceutical formulations that can be used to deliver a pharmaceutical agent, particularly a hydrophobic pharmaceutical agent. Pharmaceutical compositions for use in the present invention generally include a pharmaceutically acceptable carrier such as an excipient, diluent and/or vehicle. Delayed release formulations of compositions and delayed release systems, such as semipermeable matrices of solid hydrophobic polymers can be used.

0071A pharmaceutical formulation of a composition for use in the present invention can include a pharmaceutically acceptable carrier. The term "pharmaceutically acceptable carrier" refers to a carrier which is suitable for use in a subject without undue toxicity or irritation to the subject and which is compatible with other ingredients included in a pharmaceutical composition.

0072Pharmaceutically acceptable carriers, methods for making pharmaceutical compositions and various dosage forms, as well as modes of administration are well-known in the art, for example as detailed in <nplcit id="ncit0035" npl-type="b"><text>Pharmaceutical Dosage Forms: Tablets, eds. H. A. Lieberman et al., New York: Marcel Dekker, Inc., 1989</text></nplcit>; and in<nplcit id="ncit0036" npl-type="b"><text> L.V. Allen, Jr. et al., Ansel's Pharmaceutical Dosage Forms and Drug Delivery Systems, 8th Ed., Philadelphia, PA: Lippincott, Williams &amp; Wilkins, 2004</text></nplcit>; <nplcit id="ncit0037" npl-type="b"><text>A. R. Gennaro, Remington: The Science and Practice of Pharmacy, Lippincott Williams &amp; Wilkins, 21st ed., 2005, particularly chapter 89</text></nplcit>; and <nplcit id="ncit0038" npl-type="b"><text>J. G. Hardman et al., Goodman &amp; Gilman's The Pharmacological Basis of Therapeutics, McGraw-Hill Professional, 10th ed., 2001</text></nplcit>.

0073Pharmaceutical compositions for use according to aspects of the present invention are formulated for oral administration.

0074A solid dosage form for administration or for suspension in a liquid prior to administration illustratively includes capsules, tablets, powders, and granules. In such solid dosage forms, one or more active agents, is admixed with at least one carrier illustratively including a buffer such as, for example, sodium citrate or an alkali metal phosphate illustratively including sodium phosphates, potassium phosphates and calcium phosphates; a filler such as, for example, starch, lactose, sucrose, glucose, mannitol, and silicic acid; a binder such as, for example, carboxymethylcellulose, alignates, gelatin, polyvinylpyrrolidone, sucrose, and acacia; a humectant such as, for example, glycerol; a disintegrating agent such as, for example, agar-agar, calcium carbonate, plant starches such as potato or tapioca starch, alginic acid, certain complex silicates, and sodium carbonate; a solution retarder such as, for example, paraffin; an absorption accelerator such as, for example, a quaternary ammonium compound; a wetting agent such as, for example, cetyl alcohol, glycerol monostearate, and a glycol; an adsorbent such as, for example, kaolin and bentonite; a lubricant such as, for example, talc, calcium stearate, magnesium stearate, a solid polyethylene glycol or sodium lauryl sulfate; a preservative such as an antibacterial agent and an antifungal agent, including for example, sorbic acid, gentamycin and phenol; and a stabilizer such as, for example, sucrose, EDTA, EGTA, and an antioxidant.

0075Solid dosage forms optionally include a coating such as an enteric coating. The enteric coating is typically a polymeric material. Preferred enteric coating materials have the characteristics of being bioerodible, gradually hydrolyzable and/or gradually water-soluble polymers. The amount of coating material applied to a solid dosage generally dictates the time interval between ingestion and drug release. A coating is applied having a thickness such that the entire coating does not dissolve in the gastrointestinal fluids at pH below 3 associated with stomach acids, yet dissolves above pH 3 in the small intestine environment. It is expected that any anionic polymer exhibiting a pH-dependent solubility profile is readily used as an enteric coating in the practice of the present invention to achieve delivery of the active agent to the lower gastrointestinal tract. The selection of the specific enteric coating material depends on properties such as resistance to disintegration in the stomach; impermeability to gastric fluids and active agent diffusion while in the stomach; ability to dissipate at the target intestine site; physical and chemical stability during storage; non-toxicity; and ease of application.

0076Suitable enteric coating materials illustratively include cellulosic polymers such as hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, ethyl cellulose, cellulose acetate, cellulose acetate phthalate, cellulose acetate trimellitate, hydroxypropylmethyl cellulose phthalate, hydroxypropylmethyl cellulose succinate and carboxymethylcellulose sodium; acrylic acid polymers and copolymers, preferably formed from acrylic acid, methacrylic acid, methyl acrylate, ammonium methylacrylate, ethyl acrylate, methyl methacrylate and/or ethyl; vinyl polymers and copolymers such as polyvinyl pyrrolidone, polyvinyl acetate, polyvinylacetate phthalate, vinylacetate crotonic acid copolymer, and ethylene-vinyl acetate copolymers; shellac; and combinations thereof. A particular enteric coating material includes acrylic acid polymers and copolymers described for example <patcit id="pcit0001" dnum="US6136345A"><text>U.S. Patent No. 6,136,345</text></patcit>.

0077The enteric coating optionally contains a plasticizer to prevent the formation of pores and cracks that allow the penetration of the gastric fluids into the solid dosage form. Suitable plasticizers illustratively include, triethyl citrate (Citroflex 2), triacetin (glyceryl triacetate), acetyl triethyl citrate (Citroflec A2), Carbowax 400 (polyethylene glycol 400), diethyl phthalate, tributyl citrate, acetylated monoglycerides, glycerol, fatty acid esters, propylene glycol, and dibutyl phthalate. In particular, a coating composed of an anionic carboxylic acrylic polymer typically contains approximately 10% to 25% by weight of a plasticizer, particularly dibutyl phthalate, polyethylene glycol, triethyl citrate and triacetin. The coating can also contain other coating excipients such as detackifiers, antifoaming agents, lubricants (e.g., magnesium stearate), and stabilizers (e.g. hydroxypropylcellulose, acids or bases) to solubilize or disperse the coating material, and to improve coating performance and the coated product.

0078Liquid dosage forms for oral administration include one or more active agents and a pharmaceutically acceptable carrier formulated as an emulsion, solution, suspension, syrup, or elixir. A liquid dosage form of a composition of the present invention may include a colorant, a stabilizer, a wetting agent, an emulsifying agent, a suspending agent, a sweetener, a flavoring, or a perfuming agent.

0079For example, a composition for parenteral administration may be formulated as an injectable liquid. Examples of suitable aqueous and nonaqueous carriers include water, ethanol, polyols such as propylene glycol, polyethylene glycol, glycerol, and the like, suitable mixtures thereof; vegetable oils such as olive oil; and injectable organic esters such as ethyloleate. Proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of a desirable particle size in the case of dispersions, and/or by the use of a surfactant, such as sodium lauryl sulfate. A stabilizer is optionally included such as, for example, sucrose, EDTA, EGTA, and an antioxidant.

0080For topical administration, a composition can be formulated for administration to the skin such as for local effect, and/or as a "patch" formulation for transdermal delivery. Pharmaceutical formulations suitable for topical administration include, for example, ointments, lotions, creams, gels, pastes, sprays and powders. Ointments, lotions, creams, gels and pastes can include, in addition to one or more active agents, a base such as an absorption base, water-removable base, water-soluble base or oleaginous base and excipients such as a thickening agent, a gelling agent, a colorant, a stabilizer, an emulsifying agent, a suspending agent, a sweetener, a flavoring, or a perfuming agent.

0081Transdermal formulations can include percutaneous absorption enhancers such as acetone, azone, dimethyl acetamide, dimethyl formamide, dimethyl sulfoxide, ethanol, oleic acid, polyethylene glycol, propylene glycol and sodium lauryl sulfate. Iontophoresis and/or sonophoresis can be used to enhance transdermal delivery.

0082Powders and sprays for topical administration of one or more active agents can include excipients such as talc, lactose and one or more silicic acids. Sprays can include a pharmaceutical propellant such as a fluorinated hydrocarbon propellant, carbon dioxide, or a suitable gas. Alternatively, a spray can be delivered from a pump-style spray device which does not require a propellant. A spray device delivers a metered dose of a composition contained therein, for example, using a valve for regulation of a delivered amount.

0083Ophthalmic formulations of one or more active agents can include ingredients such as a preservative, a buffer and a thickening agent.

0084Suitable surface-active agents useful as a pharmaceutically acceptable carrier or excipient in the pharmaceutical compositions of the present invention include non-ionic, cationic and/or anionic surfactants having good emulsifying, dispersing and/or wetting properties. Suitable anionic surfactants include both water-soluble soaps and water-soluble synthetic surface-active agents. Suitable soaps are alkaline or alkaline-earth metal salts, non-substituted or substituted ammonium salts of higher fatty acids (C10-C22), e.g. the sodium or potassium salts of oleic or stearic acid, or of natural fatty acid mixtures obtainable form coconut oil or tallow oil. Synthetic surfactants include sodium or calcium salts of polyacrylic acids; fatty sulphonates and sulphates; sulphonated benzimidazole derivatives and alkylarylsulphonates. Fatty sulphonates or sulphates are usually in the form of alkaline or alkaline-earth metal salts, non-substituted ammonium salts or ammonium salts substituted with an alkyl or acyl radical having from 8 to 22 carbon atoms, e.g. the sodium or calcium salt of lignosulphonic acid or dodecylsulphonic acid or a mixture of fatty alcohol sulphates obtained from natural fatty acids, alkaline or alkaline-earth metal salts of sulphuric or sulphonic acid esters (such as sodium lauryl sulphate) and sulphonic acids of fatty alcohol/ethylene oxide adducts. Suitable sulphonated benzimidazole derivatives preferably contain 8 to 22 carbon atoms. Examples of alkylarylsulphonates are the sodium, calcium or alcanolamine salts of dodecylbenzene sulphonic acid or dibutyl-naphtalenesulphonic acid or a naphtalene-sulphonic acid/formaldehyde condensation product. Also suitable are the corresponding phosphates, e.g. salts of phosphoric acid ester and an adduct of p-nonylphenol with ethylene and/or propylene oxide, or phospholipids. Suitable phospholipids for this purpose are the natural (originating from animal or plant cells) or synthetic phospholipids of the cephalin or lecithin type such as e.g. phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerine, lysolecithin, cardiolipin, dioctanylphosphatidylcholine, dipalmitoylphoshatidyl-choline and their mixtures.

0085Suitable non-ionic surfactants useful as pharmaceutically acceptable carriers or excipients in the pharmaceutical compositions of the present invention include polyethoxylated and polypropoxylated derivatives of alkylphenols, fatty alcohols, fatty acids, aliphatic amines or amides containing at least 12 carbon atoms in the molecule, alkylarenesulphonates and dialkylsulphosuccinates, such as polyglycol ether derivatives of aliphatic and cycloaliphatic alcohols, saturated and unsaturated fatty acids and alkylphenols, said derivatives preferably containing 3 to 10 glycol ether groups and 8 to 20 carbon atoms in the (aliphatic) hydrocarbon moiety and 6 to 18 carbon atoms in the alkyl moiety of the alkylphenol. Further suitable non-ionic surfactants are water-soluble adducts of polyethylene oxide with polypropylene glycol, ethylenediaminopolypropylene glycol containing 1 to 10 carbon atoms in the alkyl chain, which adducts contain 20 to 250 ethyleneglycol ether groups and/or 10 to 100 propyleneglycol ether groups. Such compounds usually contain from 1 to 5 ethyleneglycol units per propyleneglycol unit. Representative examples of non-ionic surfactants are nonylphenol-polyethoxyethanol, castor oil polyglycolic ethers, polypropylene/ polyethylene oxide adducts, tributylphenoxypolyethoxyethanol, polyethyleneglycol and octylphenoxypolyethoxyethanol. Fatty acid esters of polyethylene sorbitan (such as polyoxyethylene sorbitan trioleate), glycerol, sorbitan, sucrose and pentaerythritol are also suitable non-ionic surfactants.

0086Suitable cationic surfactants useful as pharmaceutically acceptable carriers or excipients in the pharmaceutical compositions of the present invention include quaternary ammonium salts, preferably halides, having 4 hydrocarbon radicals optionally substituted with halo, phenyl, substituted phenyl or hydroxy; for instance quaternary ammonium salts containing as N-substituent at least one C8-C22 alkyl radical (e.g. cetyl, lauryl, palmityl, myristyl, oleyl and the like) and, as further sub-stituents, unsubstituted or halogenated lower alkyl, benzyl and/or hydroxy-lower alkyl radicals.

0087A more detailed description of surface-active agents suitable for this purpose may be found for instance in "<nplcit id="ncit0039" npl-type="b"><text>McCutcheon's Detergents and Emulsifiers Annual " (MC Publishing Crop., Ridgewood, New Jersey, 1981</text></nplcit>), "<nplcit id="ncit0040" npl-type="b"><text>Tensid-Taschenbuch", 2nd ed. (Hanser Verlag, Vienna, 1981</text></nplcit>) and "<nplcit id="ncit0041" npl-type="b"><text>Encyclopaedia of Surfactants (Chemical Publishing Co., New York, 1981</text></nplcit>).

0088Structure-forming, thickening or gel-forming agents may be included into the pharmaceutical compositions and combined preparations of the invention. Suitable such agents are in particular highly dispersed silicic acid, such as the product commercially available under the trade name Aerosil; bentonites; tetraalkyl ammonium salts of montmorillonites (e.g., products commercially available under the trade name Bentone), wherein each of the alkyl groups may contain from 1 to 20 carbon atoms; cetostearyl alcohol and modified castor oil products (e.g. the product commercially available under the trade name Antisettle).

0089In particular aspects, a pharmaceutically acceptable carrier is a particulate carrier such as lipid particles including liposomes, micelles, unilamellar or mulitlamellar vesicles; polymer particles such as hydrogel particles, polyglycolic acid particles or polylactic acid particles; inorganic particles such as calcium phosphate particles such as described in for example <patcit id="pcit0002" dnum="US5648097A"><text>U.S. Patent No. 5,648,097</text></patcit>; and inorganic/organic particulate carriers such as described for example in <patcit id="pcit0003" dnum="US6630486B"><text>U.S. Patent No. 6,630,486</text></patcit>.

0090A particulate pharmaceutically acceptable carrier can be selected from among a lipid particle; a polymer particle; an inorganic particle; and an inorganic/organic particle. A mixture of particle types can also be included as a particulate pharmaceutically acceptable carrier.

0091A particulate carrier is typically formulated such that particles have an average particle size in the range of about 1 nm - 10 microns. In particular aspects, a particulate carrier is formulated such that particles have an average particle size in the range of about 1 nm - 100 nm.

0092The dosage of an inventive pharmaceutical composition for its use will vary based on factors such as the route of administration; the age, health, sex, and weight of the subject to whom the composition is to be administered; the nature and extent of the subject's symptoms, if any, and the effect desired. Dosage may be adjusted depending on whether treatment is to be acute or continuing. One of skill in the art can determine a pharmaceutically effective amount in view of these and other considerations typical in medical practice.

0093In general it is contemplated that a daily dosage of an inventive pharmaceutical composition is in the range of about 0.001 to 100 milligrams per kilogram of a subject's body weight. A daily dose may be administered as two or more divided doses to obtain the desired effect. An inventive pharmaceutical composition may also be formulated for sustained release to obtain desired results.

0094Detailed information concerning customary ingredients, equipment and processes for preparing dosage forms is found in<nplcit id="ncit0042" npl-type="b"><text> Pharmaceutical Dosage Forms: Tablets, eds. H. A. Lieberman et al., New York: Marcel Dekker, Inc., 1989</text></nplcit>; and in<nplcit id="ncit0043" npl-type="b"><text> L.V. Allen, Jr. et al., Ansel's Pharmaceutical Dosage Forms and Drug Delivery Systems, 8th Ed., Philadelphia, PA: Lippincott, Williams &amp; Wilkins, 2004</text></nplcit>; <nplcit id="ncit0044" npl-type="b"><text>A. R. Gennaro, Remington: The Science and Practice of Pharmacy, Lippincott Williams &amp; Wilkins, 21st ed., 2005, particularly chapter 89</text></nplcit>; and <nplcit id="ncit0045" npl-type="b"><text>J. G. Hardman et al., Goodman &amp; Gilman's The Pharmacological Basis of Therapeutics, McGraw-Hill Professional, 10th ed., 2001</text></nplcit>.

0095Commercial packages include a pharmaceutical composition described herein. Instructions for administering the pharmaceutical composition may be included.

0096A commercial package is disclosed including NSC350625 or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier.

0097One or more ancillary components is optionally included in commercial packages, such as a buffer or diluent.

0098Aspects of inventive compositions and methods are illustrated in the following examples.

Examples

Reagents and Cell-based Assays

0099All cell lines were obtained from ATCC except HCT116 Bax^-/- and HCT116 p53^-/- cells obtained from Bert Vogelstein (Johns Hopkins University, Baltimore, MA) and GBM cell lines obtained from Akiva Mintz (Wake Forrest University, Winston-Salem, NC). Lentiviral infection was carried out with MDA-MB-231 cells using TRAIL shRNA or vector and HCT116 using Foxo3a shRNA or vector purchased from Sigma-Aldrich (St. Louis, MO). H460 DR5ΔDD-EGFP cells were constructed using cDNA coding for a DR5 fragment without death domain by inserting amino acids 1 to 298 of the human DR5 gene into the pEGFP-N1 vector to express a DR5(1-298)-fusion protein. The fusion construct was transfected into H460 cells with Lipofectamine 2000 (Invitrogen) and selected with G418. Positive clones were verified by florescence microscopy and Western blot analysis. Bioluminescent high-throughput screening using the NCI diversity set II was carried out in HCT116 Bax^-/- cells that were stably cotransfected to express a firefly luciferase construct under transcriptional control of the first 504 base pairs of the TRAIL promoter upstream of the start of transcription of the human TRAIL gene. Compounds were tested at a working concentration of 20 nM, 200 nM, 500 nM, and 1 µM with bioluminescent assessment of transcriptional activity at 12, 24, 36, and 48 hours post-treatment. Details of the screening methodology are described in<nplcit id="ncit0046" npl-type="s"><text> Wang et al., 2006, PNAS 103:11003-11008</text></nplcit>. NSC350625 was obtained from the NCI DTP, reconstituted in DMSO at 20 mM, aliquoted and stored at -20°C. A6730 and U0126 monoethanolate were obtained from Sigma. Purified, recombinant TRAIL was produced as described in<nplcit id="ncit0047" npl-type="s"><text> Kim et al., 2004, J. of Biol.Chem. 279:40044-40052</text></nplcit>. The RIK-2 antibody (Santa-Cruz Biotechnology) was used at 1µg/mL and zVAD-fmk (Promega) was used at 20 µM.

Primary specimens from human patients

0100All primary specimens from human patients were received immediately following resection, manually digested in complete DMEM, filtered with a 100-µm nylon mesh, and plated at 2×10⁵ cells/mL in complete DMEM for use in Examples described herein.

Mice

0101For subcutaneous xenografts, 4-6 week old female, athymic nu/nu mice (Charles River Laboratories) were inoculated with 1×10⁶ cells (2.5×10⁶ for T98G) of indicated cell lines in each rear flank as a 200 µL suspension of 1:1 Matrigel (BD):PBS. All intraperitoneal and intravenous injections were given at a total volume of 200 µL. Oral formulations of NSC350625 were administered using an oral gavage and given as a 200 µL suspension containing 20% Cremophor EL^® (Sigma), 10% DMSO, and 70% PBS. Tumors were monitored using digital calipers at indicated time points. All subcutaneous tumors were allowed to establish for 1-4 weeks post-injection until reaching a volume of ∼125 mm³ before treatment initiation. Relief of tumor burden was monitored for 3 weeks following disappearance of the tumor and confirmed by visual inspection after euthanasia.

0102Intracecal implantation was performed as described in<nplcit id="ncit0048" npl-type="s"><text> Cespedes, M.V., et al., Am J Pathol, 2007, 170(3): p. 1077-1085</text></nplcit>.

0103For intracranial xenografts, anesthetized athymic nude mice were implanted with 2×10⁵ SF767 cells in a 25µL suspension of serum- and antibiotic-free RPMI. The site of injection was a burr hole created 1mm lateral to the midline of the skull and 1mm anterior to the coronal suture. The injection was gradually administered over 5 minutes with a Hamilton syringe and the burr hole was sealed using bone wax. Tumor take was assessed by bioluminescent imaging 2 weeks following implantation. Bioluminescent imaging of tumors was carried out on an IVIS imaging system as described in<nplcit id="ncit0049" npl-type="s"><text> Wang et al., 2003, PNAS 100:15095-15100</text></nplcit>.

0104Near-infrared imaging of mice was carried out on a Pearl Impulse imaging system (LI-COR) following tail-vein injection of AngioSense^® 680 (VisEn Medical, Woburn, MA) according to the manufacturer's protocols. 6 week old Eµ-myc mice were obtained from The Jackson Laboratory (B6.Cg-Tg(IghMyc)22Bri/J).

0105For CBC/differential and serum chemistry assays, 1mL of blood was harvested from anesthetized mice by terminal cardiac puncture of the left ventricle. For serum chemistry, 500µL was placed into a microfuge tube and allowed to clot for 30 minutes at room temperature followed by centrifugation. Serum was removed, centrifuged again to remove any further clots, and serum was submitted for analysis. For CBC/differentials, 500µL of blood was collected into EDTA tubes and analyzed.

0106<b>Statistical Analyses.</b> For pair-wise comparisons, data were analyzed by the Student's two-tailed <i>t</i> test using Excel (Microsoft). Log-rank statistical analysis was performed using a web-based script that interfaces with the statistical package R.

RT-qPCR

0107Total RNA was extracted using RNeasy Minikit (Qiagen) by following the manufacturer's instructions. cDNA was generated using SuperScript II (Invitrogen) with 1 µg of RNA and oligodT. Primers were: TRAIL forward (CAGAGGAAGAAGCAACACATT, SEQ ID NO:1), TRAIL reverse (GGTTGATGATTCCCAGGAGTTTATTTTG, SEQ ID NO:2), GAPDH forward (CCACATCGCTCAGACACCAT, SEQ ID NO:3), GAPDH reverse (GGCAACAATATCCACTTTACCAGAGT, SEQ ID NO:4). PCR amplification was performed with the Applied Biosystems 7900HT Fast Real-time Detection System. Samples were standardized to 10 ng/µl and twenty ng of cDNA per sample was then utilized as a template for real-time PCR using a SYBR Green Master Mix (Qiagen Corp, USA). Samples were normalized to GAPDH used under identical conditions. Quantitation used the 2ΔΔCt method of crossing thresholds described in <nplcit id="ncit0050" npl-type="s"><text>Livak et al., 2001, Methods. 2001 Dec;25(4):402-8</text></nplcit>, with GAPDH as the endogenous control for normalization. Reactions were performed in 384 well optical plates in a 7900HT instrument (Applied Biosystems), with 10ul reaction volumes. Data analysis used the ABI PRISM 7900 Sequence Detection System 2.2 software. To exclude the possibility of genomic DNA contamination, control PCR reactions with no cDNA template and No-RT control samples were also performed for each gene-specific primer set. Quadruplicates of each PCR reaction were performed and the resultant data was averaged.

Immunofluorescence

0108Indicated cell lines were propagated in log-phase growth in six-well plates in the presence of absence of NSC350625 at indicated working concentrations for 72 hr. Cells were fixed and permeabilized using Cytofix/Cytoperm solution (BD Biosciences, San Jose, CA) solution. Cells were incubated with anti-TRAIL (ab2435, Abcam, Cambridge, MA) at 1:100 or anti-active caspase-3 (559565, BD Pharmingen, San Diego, CA) at 1:250 in Perm/Wash solution (BD Biosciences) for 1 hr in the absence of light. Anti-rabbit Alexa Fluor 488 was incubated at 1:200 in Perm/Wash solution for 20 min at room temperature and rinsed in PBS. Hoechst 33342 (Invitrogen) was used as a nuclear counterstain according to the manufacturer's protocol. Fluorescence imaging was performed on an Axiovert inverted microscope (Carl Zeiss MicroImaging) using an iVision imaging system (Biovision).

Flow cytometry and cell death assays

0109For all flow cytometry analyses, floating and adherent cells were analyzed on a Coulter-Beckman Elite Epics cytometer. For surface TRAIL experiments, adherent cells were harvested by brief trypsinization, fixed in 4% paraformaldehyde in PBS for 20 min, incubated with an anti-TRAIL antibody for 2 hr (Abcam), washed and incubated with anti-rabbit Alexafluor 488 (Invitrogen) for 30 min, and analyzed. Cells were gated on forward and side scatter to eliminate debris and dead cells from the analysis. Surface TRAIL data is expressed as median fluorescence intensity relative to that of control samples unless indicated as otherwise. For Sub-G1 and cell cycle profile experiments, all cells were pelleted and ethanol fixed followed by staining with propidium iodide (Sigma) in the presence of RNAse. Cell viability assays were carried out in 96-well black-walled clear-bottom plates using CellTiter-Glo^® (Promega) according to the manufacturer's protocols. Imaging and quantification of these assays were performed on an IVIS imaging system (Xenogen).

Colony formation assays

0110Indicated cell lines were plated at 500 cells per well and treated the following day in fresh complete media after adherence. At 3 days post-treatment, the media was replaced with drug-free media and cells were propagated for 10 days with fresh media given once every 3 days. At the end of the 10 day period, cells were washed in PBS, fixed with methanol, and stained with Coomassie blue, rinsed, and dried for quantification.

0111<b>Tissue analyses.</b> Mice were humanely sacrificed at indicated time points and excised normal tissue or tumors were fixed in 4% paraformaldehyde/PBS overnight at 4°C. If plasma samples were desired, 500 µL of blood was collected by terminal cardiac puncture under anesthesia in EDTA-Vacutainer tubes (BD). Serum samples were collected in a similar fashion but in microcentrifuge tubes followed by a 30 minute incubation at room temperature to allow for clotting. Serum was then removed following centrifugation for 5 minutes. Paraffin-embedded blocks, serial section slides, and hematoxylin and eosin staining were prepared according to standard procedures. TUNEL staining was performed using the ApopTag^® Peroxidase In Situ Apoptosis Detection Kit (Millipore). For IHC analysis, slides were dewaxed in xylene and hydrated in a decreasing gradient of ethanol. Antigen retrieval was carried out by boiling in 10 mM citric acid (pH 6.0) for 6 min. Samples were blocked with streptavidin and biotin blocking solutions and goat serum (Vector Laboratories). Primary antibodies were incubated overnight at 4°C in a humidity chamber. Incubation with biotinylated secondary antibody and DAB deposition was carried out according to the manufacturer's protocol (Vector Laboratories DAB Substrate Kit for Peroxidase). Samples were counterstained with hematoxylin (DAKO) for 6 min, rinsed in dH₂O for 5 min, rinsed with PBS, and dehydrated and sealed under cover slips. Images were recorded on an Axioskop microscope using QCapture software (QImaging).

Co-cultures

0112Co-cultures of HCT116 p53^-/- and HFF cells were performed in a 1:1 mixture of complete DMEM and McCoy's 5A medium. For fluorescence images, the two cells were separately labeled using the Fluorescent Cell Linkers Kits for gene cell membrane labeling (Sigma) according to the manufacturer's protocols. Cells were counterstained with Hoechst 33342 as described in the immunofluorescence section. For flow cytometry analysis of cell death, the two populations of cells were determined by differential light scattering and analyzed as described for sub-G1 analysis in the cell death assays section.

ELISA

0113ELISA for TRAIL was carried out using the Quantikine^® TRAIL/TNFSF10 kit according to the manufacturer's protocol (DTRL00, R&amp;D systems, Minneapolis, MN). Optical correction was performed as suggested by the manufacturer with absorbance at 540nm. Absorbances were measured with a DTX 880 plate reader (Beckman Coulter).

Pharmacokinetic analysis of NSC350625

0114The absorbance profile of NSC350625 was determined on a Gene Spec III spectrometer (Hitachi Solutions American, South San Francisco, CA). HPLC analysis was performed by absorbance detection at 239nm on an Agilent 1200 series system (Agilent, Santa Clara, CA) using an Eclipse XDB -C18 column (Agilent) and a 100µL injection loop. Isocratic elution at 1mL/minute was carried out in .1% trifluoroacetic acid in dH₂O. An acetonitrile (ACN) gradient was carried out for elution as 15-20% ACN at 0-5 minutes, 20-23% for 5-12 minutes, 25% for 12-18 minutes. The standard curve was generated by spiking concentrations of NSC350625 into plasma harvested from athymic nude mice from unrelated experiments. For all plasma samples, blood was obtained by terminal cardiac puncture of the left ventricle and collected into EDTA tubes (BD). Samples were centrifuged at 500g for 10 minutes. Plasma was deproteinated by the adding 30µL of perchloric acid to 100µL of samples, vortexed for 15 seconds, centrifuged for 2 minutes, and the supernatant was immediately injected into the HPLC. AUC was normalized to an internal serum peak with a retention time of 8.1 minutes. AUC data versus time was fit with a two-compartment open model with first order elimination from central compartment using the equation AUC = Ae^-αt + Be^-βt where t = time and A and B are the extrapolated concentrations at the initiation of the two phases (distribution and elimination). Half-lives calculated as t_1/2α=.693/α and t_1/2β=.693/β. Other equations used for calculation include CL=dose/AUC_0-∞ and V_d=dose/(AUC_0-∞Xβ).

Gene expression analysis

0115HCT116 p53^-/- cells were grown in log-phase and treated with DMSO or NSC350625 (10µM). At 48 hr, RNA was isolated using the RNeasy Mini Kit (Qiagen). Microarray analysis was performed using the Illumina HT-12 Beadchip (Illumina). RNA quality and concentration was assessed using an Agilent 2100 Bioanalyzer with RNA Nano LabChip^® (Agilent). cRNA was synthesized by TotalPrep^™ Amplification (Ambion) from 500 ng of RNA according to manufacturer's instructions. T7 oligo (dT) primed reverse transcription was used to produce first strand cDNA. cDNA then underwent second strand synthesis and RNA degradation by DNA Polymerase and RNase H, followed by filtration clean up. <i>In vitro</i> transcription (IVT) was employed to generate multiple copies of biotinylated cRNA. The labeled cRNA was purified using filtration, quantified by NanoDrop, and volume-adjusted for a total of 750 ng/sample. Samples were fragmented, and denatured before hybridization for 18 hr at 58°C. Following hybridization, beadchips were washed and fluorescently labeled. Beadchips were scanned with a BeadArray Reader (Illumina). A project was created with the resultant scan data imported into GenomeStudio 1.0 (Illumina). Results were exported to GeneSpring Gx11 (Agilent Technologies). Measurements less than 0.01 were then set to 0.01, arrays normalized to the 50^th percentile, and individual genes normalized to the median of controls. For network analysis of transcriptional changes induced by NSC350625, the dataset was analyzed using the Ingenuity Pathway Analysis software (Ingenuity Systems).

Western blot analysis

0116Western blot analysis was conducted as described in <nplcit id="ncit0051" npl-type="s"><text>Wang, W. et al., PNAS 103, 11003-11008, 2006</text></nplcit>, using NuPAGE 4-12% Bis-Tris and visualized using Supersignal West Femto (Thermo Scientific) and X-ray film. Nuclear and cytoplasmic extracts were prepared using a cytoplasmic lysis buffer (10 mM HEPES, 10 mM KCl, and 2 mM MgCl₂, 1 mM DTT) followed by a nuclear lysis buffer (20 mM HEPES, 420 mM NaCl, 1.5 mM MgCl₂, 250 µM EDTA, 25% glycerol). For all lysis buffers, fresh protease inhibitor (Roche) and 1 mM sodium orthovanadate was added immediately prior to use.

Chromatin immunoprecipitation assays

0117Chromatin immunoprecipitation (ChiP) assays were carried out as described for the TRAIL promoter in<nplcit id="ncit0052" npl-type="s"><text> Nebbioso, A., et al., Nat Med, 11(1), 77-84, 2005</text></nplcit> using a ChIP grade antibody for Foxo3a (Abcam) or an equivalent concentration of rabbit IgG (SouthernBiotech) as a nonspecific control.

NSC350625 causes p53-independent transcriptional induction of the TRAIL gene.

0118A cell-based bioluminescence reporter screen conducted in TRAIL-resistant Bax-null HCT116 human colon cancer cells using the TRAIL gene promoter yielded the small molecule NSC350625 as a TRAIL-inducing compound.

0119NSC350625 induced TRAIL promoter-dependent transcriptional activity of a luciferase reporter construct under regulatory control of the first 504 base pairs of the TRAIL promoter which excludes the p53 DNA-binding response element identified in<nplcit id="ncit0053" npl-type="s"><text> Takimoto et al., 2000, Oncogene 19, 1735-1743</text></nplcit>. <figref idref="f0001">Figure 1</figref> is a graph showing activity of luciferase reporter in HCT116 Bax^-/- cells under transcriptional control of the first 504 base pairs of the human TRAIL gene promoter upstream of the start of transcription (n=3). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and controls.

0120NSC350625 caused a dose-dependent increase in TRAIL messenger-RNA. <figref idref="f0001">Figure 2</figref> is a graph showing RT-qPCR analysis of TRAIL mRNA levels in HCT116 p53^-/- cells (48 hr, n=4). Error bars indicate s.d. of replicates.) NSC350625 caused a dose-dependent increase in TRAIL protein localized to the cell surface of several cancer cell lines in a p53-independent manner. <figref idref="f0002">Figure 3</figref> is a graph showing surface TRAIL levels induced by NSC350625 in a panel of cancer cells (10 µM, 72 hr, n=3). Error bars indicate s.d. of replicates. *P &lt; 0.05 between the indicated condition and controls.

0121NSC350625 exposure leads to a significant and sustained presence of TRAIL on the cell surface of cancer cells. A time course analysis found that TRAIL was localized to the cell surface as a late event but that this induction could be temporally sustained even after removal of NSC350625 from the media. <figref idref="f0002">Figure 4</figref> is a graph showing surface TRAIL levels in HCT116 p53^-/- cells following NSC350625 treatment at indicated conditions and time points (n=3). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and controls. <figref idref="f0002">Figure 5</figref> is a graph showing HCT116 p53^-/- TRAIL surface levels by flow cytometry at 72 hr following NSC350625 treatment initiation (5 µM, n=3). Cells were treated for the indicated time of pre-incubation and then drug-free media was exchanged for the remaining time period until analysis at 72 hr. Error bars indicate s.d. of replicates. *P &lt; 0.05 between the indicated condition and controls.

NSC350625 induces TRAIL-mediated apoptosis

0122NSC350625 induced sub-G1 DNA content that was suggestive of cell death in TRAIL-sensitive HCT116 p53^-/- cells without altering the cell cycle profiles of normal fibroblasts at equivalent doses. <figref idref="f0003">Figure 6</figref> shows cell cycle profiles of HCT116 p53^-/- and HFF cells treated with NSC350625 (5 µM, 72 hr, n=3).

0123NSC350625 decreased the clonogenic survival of cancer cell lines while sparing normal fibroblasts. <figref idref="f0003">Figure 7</figref> is a graph showing quantification of colony formation assays of cancer cells treated with NSC350625 (10 µM, 72 hr, n=3). Error bars indicate standard deviation (s.d.) of replicates. <figref idref="f0004">Figure 8</figref> is a graph showing parallel experiments as in <figref idref="f0003">Figure 7</figref> but with HFF cells that were enumerated at endpoint (n=3). Error bars indicate standard deviation (s.d.) of replicates.

0124NSC350625 induced sub-G1 content in a p53-independent and Bax-dependent manner. <figref idref="f0005">Figure 9</figref> is a graph showing sub-G1 analysis of HCT116 WT, p53^-/-, and Bax^-/- cells following treatment with DMSO, NSC350625 (1, 5, or 10 µM), or rhTRAIL (25 ng/mL) for 72 hr (n=3). Error bars indicate standard deviation (s.d.) of replicates. *P &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0125In accordance with apoptotic cell death, NSC350625 increased active caspase-3 levels as indicated by immunofluorescence assay in HCT116 p53^-/- cells treated with 5 µM NSC350625 for 72 hr and by Western blot analysis in HCT116 p53^-/- cells treated with 1 µM, 2.5 µM, 5 µM or 10 µM NSC350625 for 72 hr. <figref idref="f0005">Figure 10</figref> is an image showing Western blot analysis results. NSC350625-induced sub-G1 content was significantly inhibited by co-incubation with the pan-caspase apoptosis inhibitor zVAD-fmk. <figref idref="f0005">Figure 11</figref> is a graph showing sub-G1 analysis of NSC350625-treated cancer cells pre-incubated with or without zVAD-fmk (10 µM, 72 hr, n=3). Error bars indicate standard deviation (s.d.) of replicates. *P &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0126NSC350625-induced apoptosis appears to be specifically mediated by TRAIL, as indicated by inhibition of NSC350625-induced cytotoxicity following stable knockdown of TRAIL by shRNA. <figref idref="f0006">Figure 12</figref> is a graph showing sub-G1 analysis of MDA-MB-231 cells with stable knockdown of TRAIL by short hairpin RNA (72 hr, n=3). Error bars indicate standard deviation (s.d.) of replicates. *P &lt; 0.05 between the indicated condition and control unless otherwise indicated. <figref idref="f0006">Figure 13</figref> is a graph showing verification of MDA-MB-231 shTRAII, knockdown by flow cytometry analysis of NSC350625-treated cells (5 µM, 72 hr, n=3). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0127Additional evidence for the requirement of TRAIL in NSC350625-induced tumor cell death was observed following disruption of the DR5 death-domain that modulates proapoptotic TRAIL signaling. <figref idref="f0006">Figure 14</figref> is a graph showing sub-G1 analysis of NSC350625-induced cell death in H460 cells with endogenous DR5 or overexpression of a DR5 construct with its death domain replaced by EGFP (10 µM, 72 hr, n=3). Error bars indicate standard deviation (s.d.) of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0128Experimental sequestration of TRAIL by use of a blocking antibody showed the requirement of TRAIL in NSC350625-induced tumor cell death. <figref idref="f0007">Figure 15</figref> is a graph showing sub-G1 analysis of HCT116 cells treated with DMSO, NSC350625 (10 µM), or rhTRAIL (25 ng/mL) in the presence or absence of a TRAIL-sequestering antibody, RIK-2 (72 hr, n=3). Error bars indicate s.d. of replicates. *P &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0129The activity of NSC350625 on freshly resected colon tumor cells from a human patient was examined and it was found that NSC350625 induced TRAIL and potent cytotoxic effects unlike 5-FU. <figref idref="f0007">Figure 16</figref> is a graph showing NSC350625-induced surface TRAIL with freshly resected colon cancer cells (10 µM, 72 hr). Tissue was a mucinous adenocarcinoma resected from an 85 year-old female patient. Data is expressed as median fluorescence intensity. <figref idref="f0007">Figure 17</figref> is a graph showing results of a cell viability assay of primary colon cancer cells from <figref idref="f0007">Figure 16</figref> treated with DMSO, NSC350625 (.6, 1.25, 2.5, 5, 10, 20 µM), or 5-FU (5 µM) (n=3). Error bars indicate s.d. of replicates.

0130The cytotoxic activity of NSC350625 is thermally stable unlike TRAIL. <figref idref="f0008">Figure 18</figref> is a graph showing ability of NSC350625 (5 µM) or rhTRAIL (25 ng/mL) to reduce cell viability in HCT116 cells following a 1 hr pre-incubation at the indicated temperatures (72 hr, n=3). Error bars indicate standard deviation (s.d.) of replicates.

NSC350625 is a potent TRAIL-mediated antitumor agent <i>in vivo</i>

0131NSC350625 caused tumor regression in the HCT116 p53^-/- xenograft to a comparable extent to that observed with TRAIL when both were administered as multiple doses. <figref idref="f0008">Figure 19</figref> is a graph showing HCT116 p53^-/- xenograft treated with 3 doses of NSC350625 (i.p.), TRAIL (i.v.), or vehicle (i.p.) administered on day 0, 3, and 6 as indicated by gray vertical bars (n=10). Error bars indicate standard deviation (s.d.) of replicates. *P <i>&lt;</i> 0.05 and **P&lt;.005 between the indicated condition and control unless otherwise indicated.

0132<b>Single dose experiments in HCT116 WT and RKO human colon cancer xenograft-</b>bearing mice corroborated the potent anti-tumor activity of NSC350625, and clearly demonstrated superiority TRAIL in the RKO xenograft under the given conditions. <figref idref="f0008">Figure 20</figref> is a graph showing results of bioluminescent imaging of luciferase-infected HCT116 p53^-/- xenografts that received a single i.p. injection of NSC350625 or vehicle (n=6). Error bars indicate standard deviation (s.d.) of replicates. *<i>P</i> &lt; 0.05 and **P&lt;.005 between the indicated condition and control unless otherwise indicated.

0133<figref idref="f0009">Figure 21</figref> is a graph showing RKO xenograft with a single dose of NSC350625 (i.p.), TRAIL (i.v.), or vehicle (i.p., n=10). Error bars indicate standard deviation (s.d.) of replicates. *<i>P</i> &lt; 0.05 and **P&lt;.005 between the indicated condition and control unless otherwise indicated.

0134NSC350625 induced regression of MDA-MB-231 human breast cancer xenografts, an effect that was significantly inhibited by stable knockdown of TRAIL whereas TRAIL-treated tumors progressed. <figref idref="f0009">Figure 22</figref> is a box and whisker plot of tumor volume on day 9 following treatment initiation in MDA-MB-231 vector or shTRAIL xenografts with single doses of NSC350625 (i.p.), TRAIL (i.v.), or vehicle (DMSO, i.p.) (n=8). Error bars indicate standard deviation (s.d.) of replicates. *<i>P</i> &lt; 0.05 and **P&lt;.005 between the indicated condition and control unless otherwise indicated. TUNEL staining of tumors from the MDA-MB-231 vector and shTRAIL xenografts 2 days post-treatment with 50 mg/kg or 100 mg/kg NSC350625 show increased TUNEL staining in vector-treated no shTRAIL-treated cells.

0135This directly demonstrates that the anti-tumor activity of NSC350625 is superior to that of TRAIL when administered as single doses under these conditions and is modulated at least in part by TRAIL produced by tumor cells. In DLD-1 xenografts, NSC350625 induced tumor stasis at 1 week post-treatment while TRAIL-treated tumors progressed after a single dose. <figref idref="f0009">Figure 23</figref> is a graph showing relative tumor volume of DLD-1 xenografts treated with TRAIL (i.v.), NSC350625 (i.p.), or DMSO (i.p.) as a single dose at day 0 at indicated concentrations (n=8).

0136NSC350625 also induced a sustained regression of the SW480 xenograft as a single dose by intraperitoneal or oral delivery, suggesting favorable bioavailability. <figref idref="f0010">Figure 24</figref> is a graph showing comparison of i.p. versus oral administration of a single dose of NSC350625 at 30 mg/kg in SW480 xenografts treated on day 0 (n=6).

0137Titration of a single dose of orally administered NSC350625 in the HCT116 xenograft model revealed sustained anti-tumor efficacy at 25 mg/kg. <figref idref="f0010">Figure 25</figref> is a graph showing NSC350625 or vehicle administered as a single oral dose in the HCT116 xenograft (n=6). Error bars indicate standard deviation (s.d.) of replicates. *<i>P</i> &lt; 0.05 and **P&lt;.005 between the indicated condition and control unless otherwise indicated.

0138The lack of apparent toxicity at multiple doses delivered at 4-fold above this therapeutic dose in a previous xenograft along with no adverse effects on body weight or liver histology suggests that NSC350625 has a wide therapeutic window. <figref idref="f0010">Figure 26</figref> is a graph showing body weight of athymic, female nude mice treated with a single dose of NSC350625 (100mg/kg, i.p.). <figref idref="f0011">Figure 27</figref> is a graph showing body weight of C57B6 female mice at the end of week 4 of treatment with a single weekly dose of oral NSC350625 (25mg/kg) for 4 weeks. Histologic analysis by H&amp;E staining of liver from athymic, female nude mice harvested 3 days post-treatment with NSC350625 (100mg/kg, i.p.) showed no apparent toxicity of NSC350625.

0139Chronic exposure to oral NSC350625 at 25 mg/kg weekly for 4 weeks in immuno-competent mice did not cause any changes in a panel of serum chemistry markers as shown in Tables IA and IB. <tables id="tabl0001" num="0001"><table frame="all"><title>Tables IA and IB show serum chemistry of C57B6 mice treated with vehicle or NSC350625 (25mg/kg) weekly for 4 weeks.</title><tgroup cols="6"><colspec colnum="1" colname="col1" colwidth="30mm" align="center" /><colspec colnum="2" colname="col2" colwidth="27mm" align="center" /><colspec colnum="3" colname="col3" colwidth="27mm" align="center" /><colspec colnum="4" colname="col4" colwidth="29mm" align="center" /><colspec colnum="5" colname="col5" colwidth="23mm" align="center" /><colspec colnum="6" colname="col6" colwidth="32mm" align="center" /><thead valign="middle"><row><entry namest="col1" nameend="col6">Table IA</entry></row><row><entry>Cohort</entry><entry>Sodium (mM)</entry><entry>Potassium (mM)</entry><entry>Chloride (mM)</entry><entry>Total bilirubin (mg/dl)</entry><entry>Blood urea nitrogen (mg/dl)</entry></row></thead><tbody valign="middle"><row><entry>Control</entry><entry>151.5±4.2</entry><entry>9.025±2.2</entry><entry>106.75±1.7</entry><entry>3.075±1.6</entry><entry>26±1.6</entry></row><row><entry>NSC350625</entry><entry>154.5±5.2</entry><entry>7.325±3.2</entry><entry>104</entry><entry>2.725±2.4</entry><entry>33.75±7.3</entry></row></tbody></tgroup></table></tables><tables id="tabl0002" num="0002"><table frame="all"><tgroup cols="6"><colspec colnum="1" colname="col1" colwidth="23mm" align="center" /><colspec colnum="2" colname="col2" colwidth="25mm" align="center" /><colspec colnum="3" colname="col3" colwidth="27mm" align="center" /><colspec colnum="4" colname="col4" colwidth="18mm" align="center" /><colspec colnum="5" colname="col5" colwidth="37mm" align="center" /><colspec colnum="6" colname="col6" colwidth="38mm" align="center" /><thead valign="middle"><row><entry namest="col1" nameend="col6">Table IB</entry></row><row><entry>Cohort</entry><entry>Creatinine (mg/dl)</entry><entry>Total Protein (g/dl)</entry><entry>Albumin (g/dl)</entry><entry>Alkaline phosphate (U/L)</entry><entry>Lactate dehyrogenase (U/L)</entry></row></thead><tbody valign="middle"><row><entry>Control</entry><entry>0.25±.06</entry><entry>4.9±.36</entry><entry>3±.08</entry><entry>104.5</entry><entry>265</entry></row><row><entry>NSC350625</entry><entry>0.15±.06</entry><entry>4.97±.61</entry><entry>2.9</entry><entry>112±12</entry><entry>287.5±125</entry></row></tbody></tgroup></table></tables>

0140To test the efficacy of NSC350625 in an immuno-competent preclinical cancer model, Eµ-Myc transgenic mice that spontaneously develop lymphoma were used. The same oral dosing schedule as above that was demonstrated to be safe from weeks 9-12 of age was used. NSC350625 significantly prolonged the survival of these mice by 4 weeks. <figref idref="f0011">Figure 28</figref> is a graph showing overall survival of Eµ-myc treated during weeks 9-12 with weekly oral NSC350625 (25 mg/kg). <i>P</i> value determined by log-rank test. For relative tumor volume plots, tumor size is expressed relative to the tumor size on day 0, which is defined as the day of treatment initiation. Histologic analysis by H&amp;E staining of Eµ-myc and WT C57B6 axillary lymph nodes at 14 weeks of age showed no apparent toxicity of NSC350625.

<u>Synergistic combinations of NSC350625 and chemotherapeutic agents</u>

0141Surprisingly, <i>in vitro</i> synergy between NSC350625 and the taxanes paclitaxel and docetaxel (trade name Taxotere) is observed. <figref idref="f0011">Figure 29</figref> is a graph showing cell viability of DLD-1 treated with NSC350625 in combination with paclitaxel in at indicated conditions (72 hr, n=3). Error bars indicate s.d. of replicates. <figref idref="f0012">Figure 30</figref> is a graph showing cell viability of SW620 cells treated with NSC350625 in combination with paclitaxel in at indicated conditions (72 hr, n=3). Error bars indicate s.d. of replicates. <figref idref="f0012">Figure 31</figref> is a graph showing cell viability of DLD-1 cells treated with NSC350625 in combination with taxotere in at indicated conditions (72 hr, n=3). Error bars indicate s.d. of replicates. <figref idref="f0012">Figure 32</figref> is a graph showing cell viability of SW620 cells treated with NSC350625 in combination with taxotere in at indicated conditions (72 hr, n=3). Error bars indicate s.d. of replicates.

0142The combination of NSC350625 and either taxane paclitaxel or docetaxel cooperated to yield sustained cures in the H460 non-small cell lung cancer xenograft. <figref idref="f0013">Figure 33</figref> is a graph showing percent of cohorts in H460 xenograft that retain tumor burden following treatment with NSC350625 (30 mg/kg, i.p.) or taxotere (20 mg/kg, i.v.) alone, in combination, or with vehicle (DMSO, i.p.) (n=8) as single doses. <figref idref="f0013">Figure 34</figref> is a graph showing a relative tumor volume plot for <figref idref="f0013">Figure 33</figref>. Error bars indicate s.d. of replicates.

0143<figref idref="f0013">Figure 35</figref> is a graph showing percent of cohorts in H460 xenograft that retain tumor burden following treatment with NSC350625 (30 mg/kg, i.p.) or paclitaxel (20 mg/kg, i.v.) alone, in combination, or with vehicle (DMSO, i.p.) (n=8) as single doses. <figref idref="f0014">Figure 36</figref> is a graph showing a relative tumor volume plot for <figref idref="f0013">Figure 35</figref>. Error bars indicate s.d. of replicates.

0144NSC350625 was found in this example to cooperate with bevacizumab when both were given once a week in a metastatic orthotopic mouse model of p53-deficient colorectal cancer to reduce tumor incidence at the primary cecal tumor and distal metastatic sites including the lung, liver, lymph nodes and peritoneum. <figref idref="f0014">Figure 37</figref> is a graph showing percent of cohorts with implanted with intracecal HCT116 p53^-/- tumors with evident tumors at the primary and distal sites at endpoint (n=5). As indicated by time line, treatment was administered once a week starting at 2 weeks post-implantation with cohorts receiving vehicle, NSC350625 (25mg/kg, oral), bevacizumab (bev, 10mg/kg, i.v.), or the combination ofNSC350625 and bevacizumab.

0145NSC350625 alone and in combination with bevacizumab was well tolerated and caused no significant changes in body weight at endpoint with this multi-dose regimen. <figref idref="f0015">Figure 38</figref> is a graph showing body weight of mice at endpoint. Error bars indicate s.d. of replicates.

NSC350625 causes tumor-specific cell death by TRAIL-mediated direct and bystander effects

0146Immunohistochemical (IHC) analysis of HCT116 p53^-/- xenograft tumors following a single dose of NSC350625 on day 0 (100 mg/kg, i.p.). revealed increased protein levels of TRAIL and cleaved caspase-8, the initiator caspase involved in TRAIL-mediated apoptosis.

0147Fragmented nuclei observed by histology and increased TUNEL (TdT-mediated dUTP Nick-End Labeling) staining further confirmed that NSC350625 induced apoptosis in the treated tumors. Furthermore, NSC350625 not only induced TRAIL in the tumor but also in stromal fibroblasts bordering the tumor as shown by H&amp;E and IHC analysis for TRAIL at the border of tumor and stromal fibroblasts from HCT116 p53^-/- xenograft tumors following treatment with NSC350625 (100 mg/kg, i.p.) or vehicle on day 2 post-treatment.

0148Noting the NSC350625-induced TRAIL expression in fibroblasts, soluble TRAIL in NSC350625-treated non-tumor-bearing mice was assayed in to determine if normal cells secrete TRAIL in response to NSC350625. NSC350625 rapidly elevates serum levels of TRAIL in a manner that last for greater than 72 hours, longer than the serum half-life of recombinant TRAIL (~30 minutes). <figref idref="f0015">Figure 39</figref> is a graph showing TRAIL serum levels in tumor-free mice following NSC350625 (100 mg/kg, i.v.) or doxorubicin (30 mg/kg, i.p.) (n=2). Error bars indicate s.d. of replicates.

0149Serum TRAIL induced by NSC350625 was detected as soon as 2 hours following administration, which is more rapid that the kinetics observed in vitro in examples described herein. Pharmacokinetic analysis revealed that NSC350625 is quickly distributed and has a plasma half-life of ~6.5 hours. Table II shows results of pharmacokinetic analysis of NSC350625 in plasma of C57B6 mice. <figref idref="f0015">Figure 40</figref> is a graph showing the absorbance profile of NSC350625 with a peak absorbance at 239nm. <figref idref="f0016">Figure 41</figref> is a graph showing a calibration curve for NSC350625 spiked into mouse plasma and quantitated by HPLC analysis using area under curve (AUC). <figref idref="f0016">Figure 42</figref> is a graph showing plasma concentrations of NSC350625 following intravenous administration at 25mg/kg in C57B6 female mice (n=3). Error bars represent standard error mean of replicates. <tables id="tabl0003" num="0003"><table frame="all"><tgroup cols="12"><colspec colnum="1" colname="col1" colwidth="17mm" align="center" /><colspec colnum="2" colname="col2" colwidth="12mm" align="center" /><colspec colnum="3" colname="col3" colwidth="15mm" align="center" /><colspec colnum="4" colname="col4" colwidth="12mm" align="center" /><colspec colnum="5" colname="col5" colwidth="12mm" align="center" /><colspec colnum="6" colname="col6" colwidth="13mm" align="center" /><colspec colnum="7" colname="col7" colwidth="14mm" align="center" /><colspec colnum="8" colname="col8" colwidth="14mm" align="center" /><colspec colnum="9" colname="col9" colwidth="12mm" align="center" /><colspec colnum="10" colname="col10" colwidth="20mm" align="center" /><colspec colnum="11" colname="col11" colwidth="17mm" align="center" /><colspec colnum="12" colname="col12" colwidth="14mm" align="center" /><thead valign="middle"><row><entry namest="col1" nameend="col12">Table II</entry></row><row><entry>Dose (mg/kg)</entry><entry>t_max (h)</entry><entry>C_max (µM)</entry><entry>A (h)</entry><entry>B (h)</entry><entry>α (1/h)</entry><entry>β (1/h)</entry><entry>t_1/2α(h)</entry><entry>t_1/2β (h)</entry><entry>AUC_0-∞ (µM•h)</entry><entry>CL (L/h/kg)</entry><entry>Vd (L/kg)</entry></row></thead><tbody valign="middle"><row><entry>25</entry><entry>0.02</entry><entry>44.2</entry><entry>44.6</entry><entry>7.67</entry><entry>14.9</entry><entry>0.108</entry><entry>0.047</entry><entry>6.42</entry><entry>63.9</entry><entry>1.01</entry><entry>9.39</entry></row></tbody></tgroup></table></tables>

0150NSC350625 has a longer half-life than recombinant TRAIL and that the effects of NSC350625, i.e. TRAIL induction, are temporally sustained for days <i>in vivo</i> as seen <i>in vitro.</i>

0151IHC analysis of normal tissues in athymic, nude non-tumor bearing mice following NSC350625 administration on day 0 (100 mg/kg, i.v.) revealed that TRAIL is upregulated at the protein level in the brain, kidney, and spleen of mice without apparent toxicity as determined by histology and TUNEL staining. TRAIL upregulation in response to NSC350625 was not noted in other tissues including the liver at any time point.

0152The effects of NSC350625 on normal fibroblasts and its selectivity for normal cells was tested in this example. NSC350625 selectively induced apoptosis in p53-deficient tumor cells but not normal fibroblasts in co-culture experiments, using HCT116 p53^-/- and HFF cells treated with NSC350625 (10 µM) or DMSO for 3 days.

0153NSC350625 induces a significant though modest amount of TRAIL on the surface of normal fibroblasts. <figref idref="f0016">Figure 43</figref> is a graph showing surface TRAIL analysis of HFF cells following NSC350625 treatment (0, 2.5, 5, or 10 µM from left to right) (72 hr, n=3). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0154To test whether normal cells contribute to the anti-tumor efficacy of NSC350625 through a TRAII,-mediated bystander effect normal fibroblasts preincubated with NSC350625 were transplanted into co-culture with p53-deficient colon cancer cells. This resulted in a modest but significant increase in TRAIL-specific cell death of the cancer cell sub-population. <figref idref="f0016">Figure 44</figref> is a graph showing sub-G1 analysis of a co-culture of HCT116 p53^{- /-} cells and pretreated HFFs (24 hr, n=3). HFF pretreatment consisted of 72 hr incubation with NSC350625 (10 µM) or DMSO. These experiments were performed in the presence or absence of a TRAIL sequestering antibody (RIK-2). Scale bars are 100 µm. Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0155Thus as demonstrated herein, NSC350625 has a favorable therapeutic index and induces TRAIL in tumor, stromal, and normal cells that may contribute to the anti-tumor efficacy of NSC350625 through direct as well as bystander mechanisms.

NSC350625 is an effective antitumor agent in glioblastoma multiforme (GBM)

0156NSC350625 induces TRAIL in the brain and is useful as an anti-tumor agent against brain tumors. The activity of NSC350625 in GBM cell lines was tested in this example and it was found that NSC350625 induced TRAIL and had a p53-independent GI50 in the low micromolar range that is comparable with other cancer cell lines. <figref idref="f0017">Figure 45</figref> is a graph showing surface TRAIL in GBM cell lines following incubation with NSC350625 (5 µM, 72 hr, n=3). <i>*P</i> &lt; 0.05 between the indicated condition and control. <figref idref="f0017">Figure 46</figref> is a graph showing GI50 values extrapolated from cell viability assays of indicated GBM cell lines at 72 hr post-treatment with NSC350625 or DMSO (n=3).

0157NSC350625 has cytotoxic effects on freshly isolated GBM cells that were temozolomide-resistant and previously irradiated in this example. <figref idref="f0017">Figure 47</figref> shows results of a cell viability assay of freshly resected glioblastoma tissue treated with DMSO, NSC350625, or temozolomide (TMZ, 10 µM) (72 hr, n=3). Tissue was a grade IV glioblastoma with oligodendroglial component taken from a 38 year-old female patient who had undergone prior cytoreductive surgery and radiation.

0158NSC350625 was tested in preclinical models of GBM as a monoagent and in combination with bevacizumab. NSC350625 exerted p53-independent cytotoxicity against a panel of GBM cell lines, including temozolomide-resistant GBM cell lines such as T98G, and induced a sustained regression of subcutaneous T98G xenografts to an extent similar to bevacizumab when given as a single oral dose. <figref idref="f0018">Figure 48</figref> is a graph showing subcutaneous xenograft of T98G with mice receiving a single dose of vehicle, NSC350625 (30 mg/kg, PO), or bevacizumab (10 mg/kg, i.v.) on day 0 (n=8). *<i>P</i> &lt; 0.05 between the indicated condition and control.

0159A single dose of NSC350625 significantly doubled the overall survival of mice as a monoagent in an aggressive intracranial xenograft of human GBM using the SF767 cell line and cooperated with bevacizumab to triple the duration of survival of such brain tumor-bearing mice.

0160<figref idref="f0018">Figure 49</figref> is a graph showing overall survival of mice harboring SF767 intracranial tumors treated with a single oral dose of vehicle (n=8), NSC350625 (25 mg/kg, n=7), bevacizumab (10 mg/kg, i.v., n=6), or NSC350625 and bevacizumab (n=7) at 2 weeks post-implantation. <tables id="tabl0004" num="0004"><table frame="all"><title>Table III shows the change in overall survival of mouse cohorts with SF767 intracranial tumors.</title><tgroup cols="5"><colspec colnum="1" colname="col1" colwidth="34mm" align="center" /><colspec colnum="2" colname="col2" colwidth="19mm" align="center" /><colspec colnum="3" colname="col3" colwidth="36mm" align="center" /><colspec colnum="4" colname="col4" colwidth="40mm" align="center" /><colspec colnum="5" colname="col5" colwidth="26mm" align="center" /><thead valign="middle"><row><entry namest="col1" nameend="col5">Table III</entry></row><row><entry>Cohort</entry><entry>n</entry><entry>Median Survival (days)</entry><entry>ΔMedian Survival (days)</entry><entry><i>P</i></entry></row></thead><tbody valign="middle"><row><entry>Control</entry><entry>8</entry><entry>28</entry><entry>-</entry><entry>-</entry></row><row><entry>NSC350625</entry><entry>7</entry><entry>74</entry><entry>46</entry><entry>0.038</entry></row><row><entry>bev</entry><entry>6</entry><entry>70</entry><entry>42</entry><entry>0.119</entry></row><row><entry>NSC350625 + bev</entry><entry>7</entry><entry>96.5</entry><entry>68.5</entry><entry>0.0308</entry></row></tbody></tgroup></table></tables>

NSC350625-induced TRAIL upregulation is Foxo3a-dependent

0161To identify the molecular events underpinning NSC350625-induced upregulation of TRAIL, gene expression profiles in NSC350625-treated HCT116 p53^-/- cells were determined. Transcriptional changes in target genes of the FOXO family of transcription factors were observed, which includes Foxo3a that has been previously shown to regulate the TRAIL gene promoter at a binding site contained within the region selected for as described in <nplcit id="ncit0054" npl-type="s"><text>Modur, V. et al., 2002, J. Biol. Chem. 277:47928-47937</text></nplcit>. <figref idref="f0019">Figure 50</figref> is a graph showing transcriptional changes associated with FOXO signaling from gene expression profiling of HCT116 p53^-/- cells at 48 hr post-NSC350625 treatment (10 µM) versus DMSO (n=3). All of these changes were <i>P</i>&lt;.05 between DMSO and NSC350625 treatment groups. Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0162The FOXO-target gene DR5 was upregulated by NSC350625 in several cancer cell lines and to a much lesser extent in normal cells and this was also observed in NSC350625-treated tumors. <figref idref="f0019">Figure 51</figref> is an image of Western blot analysis of DR5 in HCT116 cells treated with NSC350625 or DMSO at indicated concentrations for 72 hr. Ran is shown as a loading control. <figref idref="f0019">Figure 52</figref> is a graph showing flow cytometry analysis of surface DR5 levels in cancer and normal cells treated with NSC350625 (72 hr, n=3). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0163IHC analysis of DR5 in HCT116 xenograft tumors treated with vehicle (i.p.) or NSC350625 (100 mg/kg, i.p.) shows, in agreement with in vitro observations that elevated DR5 expression was evident in NSC350625-treated xenograft tumors.

0164FOXO family members, Foxo3a (but not Foxo1a) underwent a nuclear translocation in response to NSC350625 as determined by immunofluorescence and Western blot analysis of Foxo3a in HCT116 cells and immunofluorescence analysis of Foxo3a in H460 and SW480 cells treated with DMSO or NSC350625, 10 µM, 48 hr.

0165<figref idref="f0020">Figure 53</figref> is an image of Western blot analysis of whole cell lysates (W) and cytoplasmic (C) and nuclear (N) extracts from HCT116 cells treated with DMSO or NSC350625 (48 hr, 10 µM). β-actin and lamin B1 are shown as cytoplasmic and nuclear loading controls, respectively.

0166A NSC350625 dose-dependent increase in the amount of Foxo3a localized to the TRAIL promoter was found as shown by chromatin immunoprecipitation assay. <figref idref="f0020">Figure 54</figref> is an image of results of a chromatin immunoprecipitation assay for NSC350625-induced translocation of Foxo3a to the TRAIL promoter at 48 hr post-NSC350625 treatment in HCT116 p53^-/- cells (0, 2.5, 5, or 10 µM from left to right).

0167Transient knockdown of Foxo3a and Foxo1 revealed that Foxo3a specifically mediated NSC350625-induced TRAIL upregulation. <figref idref="f0020">Figure 55</figref> is a graph showing results of flow cytometry analysis of cell surface TRAIL levels induced by NSC350625 (10 µM) with or without transient knockdown of Foxo1 and/or Foxo3a in HCT116 p53^-/- cells using siRNA (72 hr, n=3). Knockdown is confirmed by Western blot analysis. Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0168Stable knockdown of Foxo3a significantly inhibited NSC350625-induced upregulation of TRAIL production and subsequent tumor cell death. <figref idref="f0020">Figure 56</figref> is a graph showing sub-G1 analysis of NSC350625-induced cell death with or without stable knockdown of Foxo3a in HCT116 cells (10 µM, 72 hr, n=3). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated. <figref idref="f0021">Figure 57</figref> is a graph showing flow cytometry analysis of NSC350625-induced surface TRAIL with or without stable knockdown of Foxo3a in HCT116 cells (10 µM, 72 hr, n=3). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated. Results of Foxo3a stable knockdown were confirmed by Western blot analysis.

0169Stable knockdown of Foxo3a in tumor cells also significantly inhibited the anti-tumor activity of NSC350625 and NSC350625-induced hallmarks of TRAIL-mediated apoptosis in tumors <i>in vivo.</i><figref idref="f0021">Figure 58</figref> is a graph showing tumor volume of HCT116 xenograft with or without stable knockdown of Foxo3a following a single oral dose of vehicle or NSC350625 (25 mg/kg) on day 0 (n=10). Error bars indicate s.d. of replicates. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0170IHC analysis and TUNEL staining of HCT116 tumors with or without stable knockdown of Foxo3a 3 days after a single dose of NSC350625 (25 mg/kg, oral) was performed and showed that stable knockdown of Foxo3a in tumor cells also significantly inhibited the anti-tumor activity of NSC350625 and NSC350625-induced hallmarks of TRAIL-mediated apoptosis in tumors <i>in vivo.</i>

Dual inactivation of Akt and ERK by NSC350625 cooperatively induces TRAIL

0171NSC350625-induced changes in regulators of Foxo3a such as IKK, Akt, and ERK were determined. <figref idref="f0022">Figure 59</figref> is an image of Western blot analysis of HCT116 p53^-/- cells treated with NSC350625 (2.5, 5, 10 µM) for 72 hr.

0172Both pAkt and pERK levels were found to be abolished with NSC350625 treatment in a dose-dependent manner that was accompanied by dephosphorylation of their respective phosphorylation sites on Foxo3a. A time course analysis revealed that NSC350625-induced inactivation of Akt and ERK occurred after 48 hours, kinetics that were concerted with the dephosphorylation of Foxo3a and TRAIL upregulation. <figref idref="f0022">Figure 60</figref> is an image of Western blot analysis of HCT116 p53^-/- cells treated with NSC350625 (10 µM) for indicated time periods. <figref idref="f0023">Figure 61</figref> is a graph showing time course of protein expression levels of NSC350625-induced effects determined by densitometry of Western blots from replicate experiments as in <figref idref="f0022">Figure 60</figref> (n=3). Data is express relative to the control sample for each time point and normalized to Ran. TRAIL was quantified by flow cytometry as a parallel experiment (n=3).

0173These NSC350625-induced effects on Foxo3a were evident in several cancer cell lines of different tumor types, which include human cancer cell lines with diverse genetic backgrounds that harbor oncogenic alterations in p53, KRAS, PTEN and others. <figref idref="f0023">Figure 62</figref> is an image of Western blot analysis of NSC350625-induced effects on Foxo3a in DLD1 human colon cancer cells, MDA-MB-468 human breast cancer cells, and T98G human glioblastoma multiforme cell lines (10 µM, 72 hr).

0174Akt is found to be a determinant of cytotoxic sensitivity to NSC350625 and its TRAIL upregulation, and overactivating Akt can suppress even basal levels of TRAIL as shown by immunofluorescence analysis of Foxo3a in HCT116 cells overexpressing an empty vector or myristilated Akt (myr-Akt) with NSC350625 treatment (10 µM, 48 hr). Confirmation of overexpression of myr-Akt by Western blot analysis is shown in <figref idref="f0023">Figure 63</figref>. <figref idref="f0024">Figure 64</figref> is a graph showing flow cytometry analysis of surface TRAIL in HCT116 cells overexpressing an empty vector or myristilated Akt (myr-Akt) with NSC350625 treatment (10 µM, 48 hr). <figref idref="f0024">Figure 65</figref> is a graph showing sub-G1 content of HCT116 cells overexpressing an empty vector or myr-Akt with NSC350625 treatment (10 µM, 72 hr, n=3).

0175Dual inhibition of the Akt and the MAPK pathways will cooperatively lead to the nuclear translocation of Foxo3a and ensuing TRAIL upregulation. A6730 and U0126 monoethanolate are commercially available and previously described inhibitors of Akt1/2, <nplcit id="ncit0055" npl-type="s"><text>Desplat, V. et al., 2008, J. Enz. Inhib. Med.Chem., 23: 648-658</text></nplcit>, and MEK, <nplcit id="ncit0056" npl-type="s"><text>Favata, M. F., et al., 1998, J. Biol. Chem., 273:18623-18632</text></nplcit>, respectively used in this example to determine if dual inhibition of the Akt and the MAPK pathways will cooperatively lead to the nuclear translocation of Foxo3a and ensuing TRAIL upregulation. The combination of MEK and Akt inhibitors was found to cooperatively induce Foxo3a-dependent TRAIL upregulation and synergistically TRAIL-mediated cell death. <figref idref="f0025">Figure 66</figref> is a graph showing RT-qPCR analysis of TRAIL mRNA in HCT116 p53^-/- cells following incubation with 10 µM A6730 (Akt inh), U0126 monoethanolate (MEK inh), or both (48 hr, n=3). For Akt + MEK inh, P&lt;.05 compared to all other conditions. *<i>P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0176<figref idref="f0025">Figure 67</figref> is a graph showing surface TRAIL induction as in <figref idref="f0025">Figure 66</figref> with or without stable knockdown of Foxo3a (n=3). <i>*P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated.

0177<figref idref="f0026">Figure 68</figref> is a graph showing sub-G1 analysis of MDA-MB-231 with or without TRAIL knockdown by shRNA following incubation with 10µM Akt inh, MEK inh, or both for 48 hr (n=3). <i>*P</i> &lt; 0.05 between the indicated condition and control unless otherwise indicated. <figref idref="f0026">Figure 69</figref> is a graph showing surface TRAIL analysis of HCT116 p53^-/- cells following incubation with 10 µM A6730 (Akt inh), U0126 monoethanolate (MEK inh), or both (48 hr, n=3).

0178siRNA experiments in this example show that ERK and Akt can be inhibited to cooperatively upregulate TRAIL. <figref idref="f0027">Figure 70</figref> is a graph showing RT-qPCR analysis of TRAIL mRNA levels following transient knockdown of Akt and/or ERK in HCT116 p53^-/- cells at 48hr post-knockdown (n=3). For siERK and siAkt combination, P&lt;.05 compared to all other conditions.

0179<figref idref="f0027">Figure 71</figref> is an image showing confirmation of Akt and ERK knockdown by Western blot analysis. Error bars indicate s.d. of replicates. <figref idref="f0027">Figure 72</figref> is a graph showing surface TRAIL analysis following transient knockdown of Akt and/or ERK in HCT116 cells at 48hr post-knockdown (n=3).

0180NSC350625 causes a dual inactivation of Akt and ERK, which cooperatively leads to the nuclear translocation of their mutual substrate Foxo3a that transcriptionally induces the TRAIL gene as a unique target gene to potentiate cell death and potent anti-tumor effects in vivo.