Flocculant composition comprising ligand functionalized polymers

<u>Technical Field</u>

0001The present disclosure relates to ligand-functionalized polymers, and methods for preparing the same. The functionalized polymers are useful in selectively binding and removing biological materials, such as viruses, from biological samples.

<u>Background</u>

0002Detection, quantification, isolation and purification of target biomaterials, such as viruses and biomacromolecules (including constituents or products of living cells, for example, proteins, carbohydrates, lipids, and nucleic acids) have long been objectives of investigators. Detection and quantification are important diagnostically, for example, as indicators of various physiological conditions such as diseases. Isolation and purification of biomacromolecules are important for therapeutic uses and in biomedical research. Biomacromolecules such as enzymes which are a special class of proteins capable of catalyzing chemical reactions are also useful industrially; enzymes have been isolated, purified, and then utilized for the production of sweeteners, antibiotics, and a variety of organic compounds such as ethanol, acetic acid, lysine, aspartic acid, and biologically useful products such as antibodies and steroids.

0003In their native state in vivo, structures and corresponding biological activities of these biomacromolecules are maintained generally within fairly narrow ranges of pH and ionic strength. Consequently, any separation and purification operation must take such factors into account in order for the resultant, processed biomacromolecule to have potency.

0004The use of certain ionic polymers, especially cationic polymers, for the flocculation of cell and/or cell debris, as well as for the precipitation of proteins, is known. Similarly, ionic polymers have been used to modify filtration media to enhance the removal of impurities from process streams in depth filtration or membrane absorber type applications. The effectiveness of these flocculants is typically reduced as the conductivity of the media being processed increases, i.e. as the salt content increases. There is a need in the art for polymeric materials with increased affinity for biological species under high ionic strength conditions.

0005Chromatographic separation and purification operations can be performed on biological product mixtures, based on the interchange of a solute between a moving phase, which can be a gas or liquid, and a stationary phase. Separation of various solutes of the solution mixture is accomplished because of varying binding interactions of each solute with the stationary phase; stronger binding interactions generally result in longer retention times when subjected to the dissociation or displacement effects of a mobile phase compared to solutes which interact less strongly and, in this fashion, separation and purification can be effected.

0006Most current capture or purification chromatography is done via conventional column techniques. These techniques have severe bottlenecking issues in downstream purification, as the throughput using this technology is low. Attempts to alleviate these issues include increasing the diameter of the chromatography column, but this in turn creates challenges due to difficulties of packing the columns effectively and reproducibly. Larger column diameters also increase the occurrence of problematic channeling. Also, in a conventional chromatographic column, the absorption operation is shut down when a breakthrough of the desired product above a specific level is detected. This causes the dynamic or effective capacity of the adsorption media to be significantly less than the overall or static capacity. This reduction in effectiveness has severe economic consequences, given the high cost of some chromatographic resins.

0007Polymeric resins are widely used for the separation and purification of various target compounds. For example, polymeric resins can be used to purify or separate a target compound based on the presence of an ionic group, based on the size of the target compound, based on a hydrophobic interaction, based on an affinity interaction, or based on the formation of a covalent bond. There is a need in the art for polymeric substrates having enhanced affinity for viruses to allow selective removal from a biological sample. There is further need in the art for ligand functionalized membranes that overcome limitations in diffusion and binding, and that may be operated at high throughput and at lower pressure drops.

0008<patcit id="pcit0001" dnum="WO2009146321A"><text>WO 2009/146321</text></patcit> is directed to an article for selectively binding biological materials, the article comprising a base substrate and extending from the surfaces thereof grafted ligand groups of the formula: <chemistry id="chem0001" num="0001"><img file="EP3070472B1_D0001.tif" /></chemistry>

<u>Summary of the Invention</u>

0009The present invention is directed to a polymer flocculant comprising ligand-functionalized polymer having a carbonyl-functional (co)polymer functionalized with pendent guanidinyl groups. Such grafted ligand groups favorably have an affinity for binding neutral or negatively charged biomaterials, such as cells, cell debris, bacteria, spores, viruses, nucleic acids, and proteins.

0010In some embodiments, the ligand-functionalized polymer as a flocculant may be used whereby a biological sample, such as a cell culture fluid, is contacted causing negative and/or neutral species to bind to the polymer and precipitate from the solution or suspension.

0011The ligand functionalized polymer may be described as the reaction product of a carbonyl-functional polymer, such as diacetone (meth)acrylate (co)polymer, and a ligand compound of Formula I: <chemistry id="chem0002" num="0002"><img file="EP3070472B1_D0002.tif" /></chemistry> wherein <ul id="ul0001" list-style="none" compact="compact"><li>R² is a covalent bond, a C₂ to C₁₂ alkylene, a C₅-C₁₂ (hetero)arylene, a <chemistry id="chem0003" num="0003"><img file="EP3070472B1_D0003.tif" /></chemistry> or a <chemistry id="chem0004" num="0004"><img file="EP3070472B1_D0004.tif" /></chemistry></li><li>R⁹ is C₂ to C₁₂ alkylene or C₅-C₁₂ (hetero)arylene,</li><li>each R³ is independently H, -OH, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably H or C₁-C₄ alkyl,</li><li>R⁴ is H, C₁-C₁₂ alkyl, C₅-C₁₂ (hetero)aryl, or -N(R³)₂, preferably H, or C₁-C₄ alkyl.</li></ul>

0012A functionalized polymer is provided having grafted pendent ligand groups, said ligand groups of Formula II: <chemistry id="chem0005" num="0005"><img file="EP3070472B1_D0005.tif" /></chemistry> wherein <ul id="ul0002" list-style="none" compact="compact"><li>R¹ is H , C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably C₁-C₁₂ alkyl;</li><li>R² is a covalent bond, a C₂ to C₁₂ alkylene, or a C₅-C₁₂ (hetero)arylene, a <chemistry id="chem0006" num="0006"><img file="EP3070472B1_D0006.tif" /></chemistry> or a <chemistry id="chem0007" num="0007"><img file="EP3070472B1_D0007.tif" /></chemistry></li><li>R⁹ is C₂ to C₁₂ alkylene or C₅-C₁₂ (hetero)arylene,</li><li>each R³ is independently H, -OH, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably H or C₁-C₄ alkyl,</li><li>R⁴ is H, C₁-C₁₂ alkyl, C₅-C₁₂ (hetero)aryl, or -N(R³)₂, preferably H, or C₁-C₄ alkyl.</li></ul>

0013It will be recognized that the <chemistry id="chem0008" num="0008"><img file="EP3070472B1_D0008.tif" /></chemistry> group of Formula II is the linkage formed between the terminal amine of the ligand compound of Formula I and the carbonyl group of the carbonyl-functional polymer. With respect to the above Formula II, the "∼" represents a covalent bond or an organic linking group interposed between the ligand group and polymer chain.

0014In other embodiments, ligand functional polymer may be prepared in which the imine linking group (∼∼C(R¹)=N- is reduced to an amine linking group (∼∼CH(R¹)-NH-. This may be effected by treating the extant ligand functional polymer with a reducing agent, such as sodium cyanoborohydride, or the reduction may be effected <i>in situ</i> by added the reducing agent to the reaction mixture of the carbonyl functional polymer and the compound of Formula I.

0015In this application, (meth)acrylic is inclusive of both methacrylic and acrylic.

0016As used herein, "alkyl" or "alkylene" includes straight-chained, branched, and cyclic alkyl groups and includes both unsubstituted and substituted alkyl groups. Unless otherwise indicated, the alkyl groups typically contain from 1 to 20 carbon atoms. Examples of "alkyl" as used herein include, but are not limited to, methyl, ethyl, n-propyl, n-butyl, n-pentyl, isobutyl, t-butyl, isopropyl, n-octyl, n-heptyl, ethylhexyl, cyclopentyl, cyclohexyl, cycloheptyl, adamantyl, and norbornyl, and the like. Unless otherwise noted, alkyl groups may be mono- or polyvalent.

0017As used herein, "aryl" or "arylene" is an aromatic group containing 5-12 ring atoms and can contain optional fused rings, which may be saturated, unsaturated, or aromatic. Examples of an aryl groups include phenyl, naphthyl, biphenyl, phenanthryl, and anthracyl. Heteroaryl is aryl containing 1-3 heteroatoms such as nitrogen, oxygen, or sulfur and can contain fused rings. Some examples of heteroaryl groups are pyridyl, furanyl, pyrrolyl, thienyl, thiazolyl, oxazolyl, imidazolyl, indolyl, benzofuranyl, and benzthiazolyl. Unless otherwise noted, aryl and heteroaryl groups may be mono- or polyvalent.

<u>Brief Description of the Drawings</u>

0018<figref idref="f0001">Figure 1</figref> is a plot of the bovine serum albumin precipitation data of Example 1-5.

<u>Detailed Description of the Invention</u>

0019Polymeric flocculants comprising ligand-functionalized polymers are provided which have enhanced affinity, especially in high ionic strength media, for neutral or negatively charged biological materials such as host cell proteins, DNA, RNA, viruses, and other microorganisms. The affinity for such biomaterials allows positively charged materials, such as antibodies, to be purified, as they are not bound to the ligand functional groups. The ligand functionalized substrate allows the selective capture or binding of target biomaterials by the ligand groups, while other materials, lacking the affinity for the ligand groups are passed. The ligand functionalized polymer as a flocculant can be used to selectively bind target biomaterials, precipitate them from solution, and the precipitated adduct subsequently separated.

0020The base polymer comprises a carbonyl-functional (co)polymer; i.e. a polymer having aldehyde or ketone groups, typically pendent from the polymer chain. The polymers comprise polymerized monomer units of an ethylenically unsaturated monomer having a carbonyl group, preferably a ketone group, or copolymerized monomer units of an ethylenically unsaturated monomer and carbon monoxide (Carbon monoxide copolymers).

0021Generally, the carbonyl-functional (co)polymer is selected from the group consisting of; carbon monoxide, acrolein, vinyl methyl ketone, vinyl ethyl ketone, vinyl isobutyl ketone, isopropenyl methyl ketone, vinyl phenyl ketone, diacetone (meth)acrylamide, acetonyl acrylate, acetoacetoxyethyl (meth)acrylate, and diacetone (meth)acrylate (co)polymers. An example of a carbon monoxide containing copolymer is ELVALOY™ 741, a terpolymer of ethylene/vinyl acetate/carbon monoxide.

0022The polymer may be a copolymer of the carbonyl-functional monomer units. In particular, the carbonyl functional polymer may further comprise ethylenically unsaturated hydrophilic monomer units, and/or hydrophobic monomer units. The copolymer, in some embodiments, may be a crosslinked copolymer. In particular, the carbonyl functional copolymer may further comprise comonomer units having more than one ethylenically unsaturated groups.

0023As used herein "hydrophilic monomers" are those polymerizable monomers having a water miscibility (water in monomer) of at least 1 wt.%, preferably at least 5 weight % without reaching a cloud point, and contain no functional groups that would interfere with the binding of biological substances to the ligand group. The copolymer may comprise 0 to 90 wt.% of such monomer units in the monomer solution. When present, the polymer generally comprises 1 to 90 wt.% of such of such monomer units based on 100 wt.% total monomer.

0024The hydrophilic groups of the hydrophilic monomers may be neutral, have a positive charge, a negative charge, or a combination thereof. With some suitable ionic monomers, the ionic group can be neutral or charged depending on the pH conditions. This class of monomers is typically used to impart a desired hydrophilicity, i.e. water solubility or dispersibility to the copolymer. These comonomers are typically used to impart a desired water solubility/dispersibility of the ligand functionalized copolymer. A negatively charged comonomer may be included as long as it is in small enough amounts that it doesn't interfere with the ligand binding interaction. In applications for viral capture, the addition of a hydrophilic monomer having a positive charge at the selected pH may allow selective binding and flocculation of viruses while repelling positively charged biological materials such as antibodies.

0025Some exemplary ionic monomers that are capable of providing a positive charge are amino (meth)acrylates or amino (meth)acrylamides of Formula IV or quaternary ammonium salts thereof. The counter ions of the quaternary ammonium salts are often halides, sulfates, phosphates, nitrates, and the like. <chemistry id="chem0009" num="0009"><img file="EP3070472B1_D0009.tif" /></chemistry><ul id="ul0003" list-style="none" compact="compact"><li>where X is -O- or -NR³-;</li><li>R⁷ is independently H or CH₃,</li><li>R⁶ is a C₂ to C₁₀ alkylene, preferably C₂-C₆.</li><li>Each R⁸ is independently hydrogen, alkyl, hydroxyalkyl (i.e. an alkyl substituted with a hydroxy), or aminoalkyl (i.e. an alkyl substituted with an amino). Alternatively, the two R⁸ groups taken together with the nitrogen atom to which they are attached can form a heterocyclic group that is aromatic, partially unsaturated (i.e. unsaturated but not aromatic), or saturated, wherein the heterocyclic group can optionally be fused to a second ring that is aromatic (e.g. benzene), partially unsaturated (e.g. cyclohexene), or saturated (e.g. cyclohexane).</li></ul>

0026It will be understood with respect to Formula IV that the depicted (meth)acrylate group may be replaced by another ethylenically unsaturated group of reduced reactivity, such as methacrylate, (meth)acrylamide, vinyl, vinyloxy, allyl, allyloxy, and acetylenyl.

0027In some embodiments of Formula IV, both R⁸ groups are hydrogen. In other embodiments, one R⁸ group is hydrogen and the other is an alkyl having 1 to 10, 1 to 6, or 1 to 4 carbon atoms. In still other embodiments, at least one of R⁸ groups is a hydroxy alkyl or an amino alkyl that have 1 to 10, 1 to 6, or 1 to 4 carbon atoms with the hydroxy or amino group being positioned on any of the carbon atoms of the alkyl group. In yet other embodiments, the R⁸ groups combine with the nitrogen atom to which they are attached to form a heterocyclic group. The heterocyclic group includes at least one nitrogen atom and can contain other heteroatoms such as oxygen or sulfur. Exemplary heterocyclic groups include, but are not limited to imidazolyl. The heterocyclic group can be fused to an additional ring such as a benzene, cyclohexene, or cyclohexane. Exemplary heterocyclic groups fused to an additional ring include, but are not limited to, benzoimidazolyl.

0028Exemplary amino acrylates (i.e. X in Formula IV is oxy) include N,N-dialkylaminoalkyl (meth)acrylates such as, for example, N,N-dimethylaminoethyl(meth)acrylate, N,N-dimethylaminoethylacrylate, N,N-diethylaminoethylacrylate, N,N-dimethylaminopropyl(meth)acrylate, N-tert-butylaminopropyl(meth)acrylate, and the like.

0029Exemplary amino (meth)acrylamides (i.e. X in Formula IV is -NR³-) include, for example, N-(3-aminopropyl)methacrylamide, N-(3-aminopropyl)acrylamide, N-[3-(dimethylamino)propyl]methacrylamide, N-[3-(dimethylamino)propyl]acrylamide, N-(3-imidazolylpropyl)methacrylamide, N-(3-imidazolylpropyl)acrylamide, N-(2-imidazolylethyl)methacrylamide, N-(1,1-dimethyl-3-imidazolylpropyl)methacrylamide, N-(1,1-dimethyl-3-imidazolylpropyl)acrylamide, N-(3-benzimidazolylpropyl)acrylamide, and N-(3-benzimidazolylpropyl)methacrylamide.

0030Exemplary quaternary salts of the monomers of Formula IV include, but are not limited to, (meth)acrylamidoalkyltrimethylammonium salts (e.g. 3-methacrylamidopropyltrimethylammonium chloride and 3-acrylamidopropyltrimethylammonium chloride) and (meth)acryloxyalkyltrimethylammonium salts (e.g. 2-acryloxyethyltrimethylammonium chloride, 2-methacryloxyethyltrimethylammonium chloride, 3-methacryloxy-2-hydroxypropyltrimethylammonium chloride, 3-acryloxy-2-hydroxypropyltrimethylammonium chloride, and 2-acryloxyethyltrimethylammonium methyl sulfate).

0031Other monomers that can provide positively charged groups to the polymer include the dialkylaminoalkylamine adducts of alkenylazlactones (e.g. 2-(diethylamino)ethylamine, (2-aminoethyl)trimethylammonium chloride, and 3-(dimethylamino)propylamine adducts of vinyldimethylazlactone) and diallylamine monomers (e.g. diallylammonium chloride and diallyldimethylammonium chloride).

0032In some preferred embodiments, the second hydrophilic monomer may have an acrylate group, or other ethylenically unsaturated groups, and a poly(alkylene oxide) group; e.g. monoacrylated poly(alkylene oxide compounds, where the terminus is a hydroxy group, or an alkyl ether group. Such monomers are of the general formula: R³-O-(CH(R³)-CH₂-O)_n-C(O)-C(R³)=CH₂, V, wherein each R³ is independently H or C₁-C₄ alkyl, and n is at least 2.

0033In one embodiment, the poly(alkylene oxide) group (depicted as -(CH(R³)-CH₂-O)_n-) is a poly(ethylene oxide) (co)polymer. In another embodiment, the poly(alkylene oxide) group is a poly(ethylene oxide-co-propylene oxide) copolymer. Such copolymers may be block copolymers, random copolymers, or gradient copolymers.

0034Other representative examples of suitable hydrophilic monomers include but are not limited to acrylic acid; methacrylic acid; 2-acrylamido-2-methyl-1-propanesulfonic acid; 2-hydroxyethyl (meth)acrylate; N-vinylpyrrolidone; N-vinylcaprolactam; acrylamide; mono- or di-N-alkyl substituted acrylamide; t-butyl acrylamide; dimethylacrylamide; N-octyl acrylamide; poly(alkoxyalkyl) (meth)acrylates including 2-(2-ethoxyethoxy)ethyl (meth)acrylate, 2-ethoxyethyl (meth)acrylate, 2-methoxyethoxyethyl (meth)acrylate, 2-methoxyethyl methacrylate, polyethylene glycol mono(meth)acrylates; alkyl vinyl ethers, including vinyl methyl ether; and mixtures thereof. Preferred hydrophilic monomers include those selected from the group consisting of dimethylacrylamide, 2-hydroxyethyl (meth)acrylate and N-vinylpyrrolidinone.

0035The copolymer may further comprise hydrophobic monomer units, in amounts that do not deleteriously affect the binding performance of the ligand polymer, and the water dispersibility thereof. When present, the polymer generally comprises 1 to 20 wt.% of such monomer units based on 100 wt.% total monomer.

0036Useful classes of hydrophobic monomers include alkyl acrylate esters and amides, exemplified by straight-chain, cyclic, and branched-chain isomers of alkyl esters containing C₁ - C₃₀ alkyl groups and mono- or dialkyl acrylamides containing C₁ - C₃₀ alkyl groups. Useful specific examples of alkyl acrylate esters include: methyl acrylate, ethyl acrylate, n-propyl acrylate, n-butyl acrylate, iso-amyl acrylate, n-hexyl acrylate, n-heptyl acrylate, isobornyl acrylate, n-octyl acrylate, iso-octyl acrylate, 2-ethylhexyl acrylate, iso-nonyl acrylate, decyl acrylate, undecyl acrylate, dodecyl acrylate, lauryl acrylate, tridecyl acrylate, and tetradecyl acrylate. Useful specific examples of alkyl acrylamides include mono- and diacrylamides having pentyl, hexyl, heptyl, isobornyl, octyl, 2-ethylhexyl, iso-nonyl, decyl, undecyl, dodecyl, tridecyl, and tetradecyl groups may be used. The corresponding methacrylate esters may be used.

0037Useful classes of hydrophobic monomers further include vinyl monomers such as vinyl acetate, styrenes, and alkyl vinyl ethers, maleic anhydride and polyfunctional monomers.

0038The ligand functional polymer may be prepared by condensation of the carbonyl functional (co)polymer with a ligand compound of Formula I: <chemistry id="chem0010" num="0010"><img file="EP3070472B1_D0010.tif" /></chemistry> Wherein <ul id="ul0004" list-style="none" compact="compact"><li>R² is a covalent bond, a C₂ to C₁₂ alkylene, a C₅-C₁₂ (hetero)arylene, a <chemistry id="chem0011" num="0011"><img file="EP3070472B1_D0011.tif" /></chemistry> or a <chemistry id="chem0012" num="0012"><img file="EP3070472B1_D0012.tif" /></chemistry></li><li>R⁹ is C₂ to C₁₂ alkylene or C₅-C₁₂ (hetero)arylene,</li><li>each R³ is independently H, -OH, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably H or C₁-C₄ alkyl,</li><li>R⁴ is H, C₁-C₁₂ alkyl, C₅-C₁₂ (hetero)aryl, or -N(R³)₂, preferably H, or C₁-C₄ alkyl.</li></ul>

0039The resulting polymer will have pendent guanidinyl groups of the formula: <chemistry id="chem0013" num="0013"><img file="EP3070472B1_D0013.tif" /></chemistry> wherein <ul id="ul0005" list-style="none" compact="compact"><li>R¹ is H, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl,</li><li>R² is a covalent bond, a C₂ to C₁₂ alkylene, a C₅-C₁₂ (hetero)arylene, a <chemistry id="chem0014" num="0014"><img file="EP3070472B1_D0014.tif" /></chemistry> or a <chemistry id="chem0015" num="0015"><img file="EP3070472B1_D0015.tif" /></chemistry></li><li>R⁹ is C₂ to C₁₂ alkylene or C₅-C₁₂ (hetero)arylene,</li><li>each R³ is independently H, -OH, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably H or C₁-C₄ alkyl,</li><li>R⁴ is H, C₁-C₁₂ alkyl, C₅-C₁₂ (hetero)aryl, or -N(R³)₂, preferably H, or C₁-C₄ alkyl.</li></ul>

0040More particularly, the pendent ligand groups will be of the formula: <tables id="tabl0001" num="0001"><img file="EP3070472B1_D0016.tif" /></tables><ul id="ul0006" list-style="none" compact="compact"><li>R⁹ is C₂ to C₁₂ alkylene or C₅-C₁₂ (hetero)arylene,</li><li>each R³ is independently H, -OH, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably H or C₁-C₄ alkyl,</li><li>R⁴ is H, C₁-C₁₂ alkyl, C₅-C₁₂ (hetero)aryl, or -N(R³)₂, preferably H, or C₁-C₄ alkyl.</li></ul>

0041The reaction may be illustrated as follows:         -(M^CO)_w-(M^Hydrophil)_x-(M^hydrophob)_z- → -(M^Lig)_y-(M^Hydrophil)_x--(M^hydrophob)_z-(M^CO)_w*-, where <ul id="ul0007" list-style="none" compact="compact"><li>-(M^CO)_w are carbonyl functional monomer units having "w" polymerized monomer units,</li><li>-(M^Hydrophil)_x- are hydrophilic monomer units having "x" polymerized monomer units,</li><li>-(M^hydrophob)_z- are hydrophobic monomer units having "z" polymerized monomer units (M^Lig)_y are ligand functional monomer units having "y" polymerized monomer units, where y is less than or equal to w; i.e. all or a portion of the carbonyl groups are functionalized by the ligand compound of Formula I. The w, x and z subscripts correspond to the weight ranges of the monomers used: w may comprise 10 to 100 wt.% of the monomer mixture, x may comprise 0 to 90 wt.% of the monomer mixture, and z may comprise 0 to 20 wt. % of the monomer mixture, "y" indicates the number of carbonyl functional groups functionalized with the ligand groups, and w* indicates the number of unfunctionalized carbonyl groups.</li></ul>

0042Alternatively to functionalizing the carbonyl functional polymer with a ligand compound of formula I, the ligand functional polymer may be prepared by polymerizing a monomer of the formula: <chemistry id="chem0016" num="0016"><img file="EP3070472B1_D0017.tif" /></chemistry> wherein <ul id="ul0008" list-style="none" compact="compact"><li>R¹ is H, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl,</li><li>R² is a covalent bond, a C₂ to C₁₂ alkylene, a C₅-C₁₂ (hetero)arylene, a <chemistry id="chem0017" num="0017"><img file="EP3070472B1_D0018.tif" /></chemistry> or a <chemistry id="chem0018" num="0018"><img file="EP3070472B1_D0019.tif" /></chemistry></li><li>R⁹ is C₂ to C₁₂ alkylene or C₅-C₁₂ (hetero)arylene,</li><li>each R³ is independently H, -OH, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably H or C₁-C₄ alkyl,</li><li>R⁴ is H, C₁-C₁₂ alkyl, C₅-C₁₂ (hetero)aryl, or -N(R³)₂, preferably H, or C₁-C₄ alkyl.</li><li>X is -O- or -NR³-</li><li>R⁶ is a C₂ to C₁₂ alkylene, and</li><li>R⁷ is H or CH₃.</li></ul>

0043The monomer of Formula VI may be copolymerized with the hydrophilic monomers previously described.

0044Alternatively, the ligand functional polymer may be prepared by polymerizing a monomer of Formula VII. The monomer of Formula VII may be copolymerized with the hydrophilic monomers previously described. <chemistry id="chem0019" num="0019"><img file="EP3070472B1_D0020.tif" /></chemistry> wherein <ul id="ul0009" list-style="none" compact="compact"><li>R¹ is H, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl,</li><li>R² is a covalent bond, a C₂ to C₁₂ alkylene, a C₅-C₁₂ (hetero)arylene, a <chemistry id="chem0020" num="0020"><img file="EP3070472B1_D0021.tif" /></chemistry> or a <chemistry id="chem0021" num="0021"><img file="EP3070472B1_D0022.tif" /></chemistry></li><li>R⁹ is C₂ to C₁₂ alkylene or C₅-C₁₂ (hetero)arylene,</li><li>each R³ is independently H, -OH, C₁-C₁₂ alkyl, or C₅-C₁₂ (hetero)aryl, preferably H or C₁-C₄ alkyl,</li><li>R⁴ is H, C₁-C₁₂ alkyl, C₅-C₁₂ (hetero)aryl, or -N(R³)₂, preferably H, or C₁-C₄ alkyl, and R⁷ is H or CH₃.</li></ul>

0045The disclosure further provides a functionalized substrate comprising a base substrate and a grafted or ungrafted coating of the ligand functionalized polymer thereon. Preferably the base substrate is a porous base substrate having interstitial and outer surfaces.

0046The base substrate may be formed from any suitable metallic, thermoplastic, or thermoset material. The material may be an organic or inorganic polymeric material. Suitable organic polymeric materials include, but are not limited to, poly(meth)acrylates, poly(meth)acrylamides, polyolefins, poly(isoprenes), poly(butadienes), fluorinated polymers, chlorinated polymers, polyamides, polyimides, polyethers, poly(ether sulfones), poly(sulfones), poly(vinyl acetates), copolymers of vinyl acetate, such as poly(ethylene)-co-poly(vinyl alcohol), poly(phosphazenes), poly(vinyl esters), poly(vinyl ethers), poly(vinyl alcohols), and poly(carbonates). Suitable inorganic polymeric materials include, but are not limited to, quartz, silica, glass, diatomaceous earth, and ceramic materials.

0047Suitable polyolefins include, but are not limited to, poly(ethylene), poly(propylene), poly(1-butene), copolymers of ethylene and propylene, alpha olefin copolymers (such as copolymers of ethylene or propylene with 1-butene, 1-hexene, 1-octene, and 1-decene), poly(ethylene-<i>co</i>-1-butene) and poly(ethylene-<i>co</i>-1-butene-co-1-hexene).

0048Suitable fluorinated polymers include, but are not limited to, poly(vinyl fluoride), poly(vinylidene fluoride), copolymers of vinylidene fluoride (such as poly(vinylidene fluoride-<i>co</i>-hexafluoropropylene), and copolymers of chlorotrifluoroethylene (such as poly(ethylene-<i>co</i>-chlorotrifluoroethylene).

0049Suitable polyamides include, but are not limited to, poly(iminoadipoyliminohexamethylene), poly(iminoadipoyliminodecamethylene), and polycaprolactam. Suitable polyimides include, but are not limited to, poly(pyromellitimide).

0050Suitable poly(ether sulfones) include, but are not limited to, poly(diphenylether sulfone) and poly(diphenylsulfone-<i>co</i>-diphenylene oxide sulfone).

0051Suitable copolymers of vinyl acetate include, but are not limited to, poly(ethylene-<i>co</i>-vinyl acetate) and such copolymers in which at least some of the acetate groups have been hydrolyzed to afford various poly(vinyl alcohols).

0052The base substrate may be in any form such as particles or fibers. Suitable particles include, but are not limited to, magnetic particles, organic particles, inorganic particles, and porous and nonporous particles. Preferably the base substrate is porous. Suitable porous base substrates include, but are not limited to, porous particles and porous fibers.

0053In some embodiments, the porous base substrate is formed from propylene homo- or copolymers, most preferably propylene homopolymers. Polypropylene polymers are often a material of choice for porous articles due to properties such as non-toxicity, inertness, low cost, and the ease with which it can be extruded, molded, and formed into articles.

0054In many embodiments, the porous base substrate has an average pore size that is typically greater than about 0.2 micrometers in order to minimize size exclusion separations, minimize diffusion constraints and maximize surface area and separation based on binding of a target molecule. Generally, the pore size is in the range of 0.1 to 10 micrometers, preferably 0.5 to 3 micrometers and most preferably 0.8 to 2 micrometers when used for binding of viruses. The efficiency of binding other target molecules may confer different optimal ranges.

0055Suitable porous base substrates include, but are not limited to, porous and microporous particles and fibers.

0056In one embodiment the base substrate may have a coating of the ligand functional (co)polymer on a surface thereon. Useful coating techniques include applying a solution or dispersion of the (co)polymer, optionally including a crosslinker, onto the base substrate. Polymer application is generally followed by evaporating the solvent to form the polymer coating. Coating methods include the techniques commonly known as dip, spray, knife, bar, slot, slide, die, roll, or gravure coating. Coating quality generally depends on mixture uniformity, the quality of the deposited liquid layer, and the process used to dry or cure the liquid layer.

0057In some embodiments, the carbonyl functional (co)polymer is first coated on the base substrate and subsequently reacted with the ligand compound of Formula I. Useful crosslinkers for the coating operation in these instances include carbonyl reactive compounds such as polyamines, polyhydrazines, and polyhydrazides.

0058In other embodiments, the ligand functional (co)polymer itself is coated on the base substrate. Useful crosslinkers in these instances include amine reactive compounds such as bis- and polyepoxides, polycarboxylic acids and their derivatives (e.g., acid chlorides), polyisocyanates, and formaldehyde-based crosslinkers such as hydroxymethyl and alkoxymethyl functional crosslinkers, such as those dervided from urea or melamine.

0059In other embodiments, the ligand functional copolymer is coated on the base substrate by polyelectrolyte layer-by-layer coating techniques, such as those described in <patcit id="pcit0002" dnum="EP472990A"><text>EP 472,990</text></patcit>.

0060In another embodiment, the ligand-functional polymer may be grafted to the surface of the base substrate; i.e. a covalent bond is formed between the ligand functional polymer and the polymer base substrate. The covalent bond may be formed by displacement, condensation or free radical methods. The nature of the grafting depends on the type of polymer used for the base substrate.

0061In some embodiments, the base polymer has carbonyl-reactive functional groups, such as amines on the surface thereof. These surface functional groups may react with extant carbonyl functional groups on the ligand functional polymer. In another embodiment, the surface of the substrate may be provided with amine-reactive functional groups, such as halide, epoxy, ester, isocyanate groups, that can react with the guanidino groups of the ligand functionalized polymer.

0062In some embodiments the polymer may be grafted to the surface of a substrate by ionizing radiation-initiated graft polymerization of a monomer such as those of Formulas VI or VII, optionally with other hydrophilic or hydrophobic monomers previously described. Alternatively, a carbonyl functional monomer may be grafted to the surface of a substrate by ionizing radiation-initiated graft polymerization, followed by functionalization by reaction with a ligand compound of Formula I.

0063In some embodiments, the surface of the substrate may be free radically functionalized with a grafting monomer having a free-radically polymerizable group and a second functional group reactive with the ligand functional polymer. Such monomers may include isocyanatoethyl (meth)acrylate or glycidyl (meth)acrylate.

0064The grafting monomers can graft (i.e. forming a covalent bond) to the surface of the base substrate when exposed to an ionizing radiation preferably e-beam or gamma radiation. That is, reaction of (meth)acryloyl groups of the grafting monomers with the surface of the porous base substrate in the presence of the ionizing radiation results in the reaction of ethylenically unsaturated free-radically polymerizable groups being directly grafted to the base substrate via the acrylate group, and further provides the surface of the substrate with a reactive functional groups that may be subsequently reacted with the ligand functional polymer.

0065The methods of the present disclosure involve the irradiation of porous or nonporous substrate surfaces with ionizing radiation to prepare free radical reaction sites on such surfaces upon which the functional monomers are grafted. The functional groups of the functional monomers then allow the ligand functional polymer to be grafted to the surface of the substrate. "Ionizing radiation" means radiation of a sufficient dosage and energy to cause the formation of free radical reaction sites on the surface(s) of the base substrate. Ionizing radiation may include beta, gamma, electron-beam, x-ray and other electromagnetic radiation. In some instances, corona radiation can be sufficiently high energy radiation. The radiation is sufficiently high energy, that when absorbed by the surfaces of the base substrate, sufficient energy is transferred to that support to result in the cleavage of chemical bonds in that support and the resultant formation of a free radical site on the support.

0066The method of grafting (or coating) a ligand functionalized polymer to the surface of the substrate alters the original nature of the base substrate, as the substrate bears a grafted or ungrafted coating of the ligand functional polymer. The present invention enables the formation of ligand functionalized polymer substrates having many of the advantages of a base substrate (e.g., mechanical and thermal stability, porosity), but with enhanced affinity for biological species such as viruses, resulting from the monomers and steps used to form a given functionalized substrate.

0067The porous substrates having a coating of ligand-functionalized polymer are particularly suited as filter media, for the selective binding and removal of contaminating proteins, cells, cell debris, microbes, nucleic acids, and/or viruses from biological samples. The present disclosure further provides a method for the removal of target biological species from a biological sample by contacting the sample with the ligand polymer functionalized substrate as described herein.

0068The ligand functionalized (co)polymer (either the polymer per se, or a substrate having a coating thereof) is useful for the purification of biological or other fluid samples comprising biologically derived species (biological species). Biological species include, but are not limited to, cells, cell debris, proteins, nucleic acids, endotoxins, and viruses. Cells and cell debris include those derived from archaea, bacteria, and eucaryota. Bacteria include, but are not limited to, Gram-negatives such as <i>Pseudomonas</i> species, <i>Escherichia coli</i>, <i>Helicobacter pylori</i>, and <i>Serratia marcesens</i>; Gram-positives such as <i>Staphylococcus</i> species, <i>Enterococcus</i> species, <i>Clostridium</i> species, <i>Bacillus</i> species, and <i>Lactobacillus</i> species; bacteria that do not stain traditionally by Gram's method such as <i>Mycobacterium</i> species, and non-vegetative forms of bacteria such as spores. Eucaryota include, but are not limited to, animal cells, algae, hybridoma cells, stem cells, cancer cells, plant cells, fungal hyphae, fungal spores, yeast cells, parasites, parasitic oocysts, insect cells, and helminthes. Proteins, include, but are not limited to, natural proteins, recombinant proteins, enzymes, and host cell proteins. Viruses include, but are not limited to, enveloped species such as Herpesviruses, Poxviruses, Adenoviruses, Papovaviruses, Coronaviruses, retroviruses such as HIV, and Plasmaviridae; and non-enveloped species such as Caliciviridae, Corticoviridae, Myoviridae, and Picornaviridae.

0069In some embodiments, the biological species being removed from the fluid is the object of the purification. For example, a recombinant protein or enzyme may be prepared in cell culture, the (co)polymer can be added to flocculate the protein or enzyme, and the precipitate can be separated as the first step in the purification process for the protein or enzyme. In another example, the (co)polymer or a substrate with a coating thereof, may be used to capture microorganisms from a fluid as the first step in a process of concentrating, enumerating, and/or identifying the microorganisms.

0070In other embodiments, the biological species being removed from the fluid is a contaminant that must be removed prior to additional processing steps for the fluid. The polymer as a flocculant can be used to facilitate the removal of cells and cell debris from a cell culture or fermentation broth prior to, subsequent to, or in place of a centrifuge or depth filtration operation. For example, the (co)polymer can be used to flocculate cells in a cell culture broth prior to centrifugation, and thereby improve the efficiency with which the centrifugation process separates the cell mass from the liquid centrate. Alternatively, it can be added to the liquid centrate after a centrifugation step to flocculate suspended cell debris and dissolved host cell proteins and nucleic acids, thereby increasing the efficiency of a subsequent depth filtration step. It can be used to flocculate or precipitate suspended bacteria, viruses, or other microorganisms. It can be used to precipitate either desired or contaminating proteins or nucleic acids from solution. Significantly, the ligand functional (co)polymers, or substrates having a coating thereof, are useful under conditions of high salt concentration or high ionic strength , i.e., they are "salt tolerant". The term "salt" is meant to include all low molecular weight ionic species which contribute to the conductivity of the solution. The importance of utility of the ligand functional (co)polymers in the presence of salt is that many process solutions used in biopharmaceutical or enzyme manufacture have conductivities in the range of 15-30 mS/cm (approximately 150-300 mM salt) or more. Salt tolerance can be measured in comparison to that of the conventional quaternary amine or Q ligand (e.g. trimethylammonium ligand), whose primarily electrostatic interactions with many biological species rapidly deteriorates at conductivities three- to six-fold less than the target range. For example, membranes derivatized with the conventional Q ligand exhibit a drop in ϕX174 viral clearance from a six log-reduction value (LRV) to a one (1) LRV in going from 0 to 50 mM NaCl (ca. 5-6 mS/cm conductivity). Viruses such as ϕX174 which have pIs close to 7 (are neutral or near-neutral) are extremely difficult to remove from process streams. Similar problems are observed when attempting to remove other biological species from process fluids. For example, when attempting to remove positively charged proteins such as host cell proteins through the use of filtration devices functionalized with conventional Q ligands, the process fluid may have to be diluted two-fold or more in order to reduce the conductivity to an acceptable range. This is expensive and dramatically increases the overall processing time.

0071Used as a flocculant, the amount of ligand functional (co)polymer that is added relative to the amount of sample can vary over a wide range. Generally, the amount added will produce a final concentration of (co)polymer in the mixture of from about 0.01 micrograms/mL to about 5000 micrograms/mL. The optimal amount of (co)polymer added will depend upon the concentration of the species one desires to flocculate. Typically, the amount of polymer relative to the amount of species being flocculated will be in the range of 0.01% to 100% by weight, preferably 0.05%-30% by weight, more preferably about 0.1%-10% by weight. The optimal amount is readily assessed by challenging the sample with a series of polymer concentrations as is well known in the art. While the above concentration ranges are typical, one skilled in the art will realize that other ranges may work in some instances. Flocculation efficiency also depends upon the physical and chemical characteristics of the species being flocculated. For example, we have found that optimal flocculation of the near neutral virus ϕX174 from aqueous suspension occurs at a polymer to virus weight ratio of about 800-1000%.

0072The biological sample is contacted with the ligand functionalized polymer (either the polymer per se, or a substrate having a coating thereof) for a time sufficient to interact and form a complex with the target biological species disposed (dissolved or suspended) in the solution when the solution comprises from 0 to about 50 mM salt, preferably when the solution comprises from 0 to about 150 mM salt, more preferably when the solution comprises from 0 to about 300 mM salt or higher, such that the concentration of the target biological species remaining disposed in the solution is less than 50% of its original concentration. It is more preferred that the solution is contacted with the ligand functionalized polymer for a time sufficient to interact and form a complex with the target biological species disposed in the solution when the solution comprises from 0 to about 50 mM salt, preferably when the solution comprises from 0 to about 150 mM salt, more preferably when the solution comprises from 0 to about 300 mM salt or higher, such that the concentration of the target biological species remaining disposed in the solution is less than 10% of its original concentration. It is still more preferred that the solution is contacted with the ligand functionalized polymer for a time sufficient to interact and form a complex with the target biological species disposed in the solution when the solution comprises from 0 to about 50 mM salt, preferably when the solution comprises from 0 to about 150 mM salt, more preferably when the solution comprises from 0 to about 300 mM salt or higher, such that the concentration of the target biological species remaining disposed in the solution is less than 1% of its original concentration.

0073In many embodiments the ligand functionalized (co)polymer, being positively charged in aqueous media, will bind near neutral or negatively charged species to the ligand functional group of Formula II while other species (e.g., positively charged proteins such as monoclonal antibodies) will be excluded or repelled from the ligand functionalized substrate. In addition, as previously described, the substrate may be directly or indirectly grafted with one or more ionic monomers. In particular, the ligand functionalized polymer may comprise grafted ionic groups that are positively charged at the selected pH of the biological sample solution to enhance electrostatic charge repulsion of proteins, such as monoclonal antibodies, many of which are charged positive at neutral pH, and ligand functional groups of Formula II to provide salt tolerance.

0074In some embodiments the ligand functionalized polymer and coated substrate containing the bound biological species are disposable. In such embodiments, the binding of the biological species to the ligand functionalized polymer is preferably essentially irreversible because there is no need to recover the bound biological species. Nonetheless, if desired, one can reverse the binding of biological species by increasing the ionic strength or changing the pH of an eluting solution.

<u>Examples</u>

Examples 1-5.

0075Diacetone acrylamide (40 grams), ethanol (60 grams) and VAZO 67 (0.2 gram) were charged to a 236.6 ml (8 ounce) glass bottle. The mixture was purged with a slow stream of nitrogen gas for 5 minutes, sealed, and then tumbled in a water bath equilibrated to 55°C for 24 hours to convert the monomer to polymer. A portion of this polymer solution (4.07 grams) was diluted with methanol (16.48 grams) and mixed with aminoguanidine hydrochloride (1.08 grams, TCI America, Portland, OR). Trifluoroacetic acid (4 drops) was added as catalyst, and the solution was mixed for 2 hours at ambient temperature. IR and ¹H-NMR analyses confirmed the formation of poly(diacetoneacrylamide guanylhydrazone) (polymer 1).

0076By similar procedures, polymers <u>2-5</u> were prepared by converting 20, 40, 60, and 80% of the repeat units to guanylhydrazone functionality.

Examples 6-10.

0077Copolymers of dimethylacrylamide and diacetoneacrylamide were prepared in methanol solution by the procedure described in Example 1. The copolymers, containing 20, 40, 60, 80 and 0% by weight dimethylacrylamide, were reacted with aminoguanidine by the procedure described in Example 1 except that concentrated hydrochloric acid was used as catalyst instead of trifluoroacetic acid, to produce the corresponding guanylhydrazone copolymers <u>6-10.</u>

Example 11.

0078Guanylhydrazone polymers 1-5 were assayed for BSA precipitation according to the following procedure. Results are shown in <figref idref="f0001">Figure 1</figref>.

0079A solution of bovine serum albumin (BSA, Sigma-Aldrich) was prepared in 10 mM MOPS buffer (4-morpholinepropanesulfonic acid), pH 7.5, and determined to have a concentration of BSA of 4.0 mg/mL. A series of BSA solutions were prepared containing various concentrations of sodium chloride according to the following Table 1: <tables id="tabl0002" num="0002"><table frame="all"><title>Table 1</title><tgroup cols="4"><colspec colnum="1" colname="col1" colwidth="29mm" /><colspec colnum="2" colname="col2" colwidth="31mm" /><colspec colnum="3" colname="col3" colwidth="25mm" /><colspec colnum="4" colname="col4" colwidth="29mm" /><thead><row><entry align="center" valign="top">[NaCl] (mM, final)</entry><entry align="center" valign="top">BSA solution (mL)</entry><entry align="center" valign="top">5 M NaCl (µL)</entry><entry align="center" valign="top">MOPS buffer (µL)</entry></row></thead><tbody><row rowsep="0"><entry colsep="0" align="center">0</entry><entry colsep="0" align="center">10</entry><entry colsep="0" align="center">0</entry><entry align="center">500</entry></row><row rowsep="0"><entry colsep="0" align="center">50</entry><entry colsep="0" align="center">10</entry><entry colsep="0" align="center">100</entry><entry align="center">400</entry></row><row rowsep="0"><entry colsep="0" align="center">100</entry><entry colsep="0" align="center">10</entry><entry colsep="0" align="center">200</entry><entry align="center">300</entry></row><row rowsep="0"><entry colsep="0" align="center">150</entry><entry colsep="0" align="center">10</entry><entry colsep="0" align="center">300</entry><entry align="center">200</entry></row><row rowsep="0"><entry colsep="0" align="center">200</entry><entry colsep="0" align="center">10</entry><entry colsep="0" align="center">400</entry><entry align="center">100</entry></row><row><entry colsep="0" align="center">250</entry><entry colsep="0" align="center">10</entry><entry colsep="0" align="center">500</entry><entry align="center">0</entry></row></tbody></tgroup></table></tables>

0080Solutions of the polymers from Examples 1-5 were diluted with deionized water to 1% solids by weight and adjusted to pH 7.

0081A 5 mL polypropylene centrifuge tube was charged with 2.0 mL of BSA solution, followed by 125 µL of diluted polymer solution. The centrifuge tube was sealed and tumbled end over end for 30 minutes, then centrifuged at 2000 rcf for 10 minutes. A BSA standard solution was prepared by mixing 2 mL of original BSA solution with 125 µL of MOPS buffer. A serial 1:1 dilution was performed to provide a total of 7 BSA standards. These seven standards were pipetted (200 µL) in triplicate into wells of a 96-well microtitration plate, along with triplicate samples of the supernates from each polymeric flocculant being evaluated. Three wells containing DI water as a blank were also included. The plate was analyzed using a SpectraMAX 250 Microplate Spectrophotometer System (Molecular Devices Corp, Sunnyvale, CA) using a wavelength of 293 nm. Comparison of the absorptions of the flocculant solutions to those of the standards provided a measure of the percentage of BSA precipitated.

Comparative Example 1. Poly(Methacrylamidopropyltrimethylammonium Chloride) (pMAPTAC)

0082MAPTAC (160 grams of a 50% by weight solution in water, from Aldrich, Milwaukee, WI), ethanol (40 grams) and sodium persulfate (0.4 gram) were charged to a 473.2 ml (16 ounce) glass bottle. The mixture was purged with a slow stream of nitrogen gas for 10 minutes, sealed, and then tumbled in a water bath equilibrated to 55°C for 24 hours to convert the monomer to polymer. This polymer solution was diluted with deionized water (80 grams) and ethanol (40 grams) and mixed well. A sample for evaluation as a flocculant was prepared by dilution of a portion of this polymer to 1% solids by weight with deionized water, pH 7. A BSA precipitation test was conducted as described in Example 11, and the pMAPTAC was found to be a good flocculator at 0 mM NaCl, but it left 42%, 73%, and 100% of the BSA in solution at 50 mM, 100 mM, and 150 mM NaCl, respectively.

Example 12. Virus Flocculation

0083Aqueous suspensions of ϕX174 bacteriophage (ca. 10⁹ pfu/mL) were prepared in 10 mM TRIS((hydroxymethyl)aminomethane) pH 8.0 containing 0, 50 mM, and 150 mM NaCl. Aqueous solutions of flocculator polymers were prepared in DI water, pH 7, at 0.001% polymer by weight. 16 µL of polymer solution were added to a 2mL sample of bacteriophage suspension in a centrifuge tube. The tube was sealed, vortexed, and rotated end-over-end for 2 hours. The tubes were then centrifuged at 3000 rcf for 10 minutes, and the resultant suspensions were filtered through a 0.45 µm sterile syringe filter (GHP Acrodisc, Pall Life Sciences). A 10-fold dilution series was prepared.

0084One mL of each dilution was mixed with 1 mL E. coli culture (grown to an optical density of 0.3-0.6 when measured at 550 nm). After waiting 5 minutes, a sterile pipet was used to mix 4.5 mL TSA Top agar with the dilution/E. coli mixture and plated on TSB plates. After the top agar had solidified, the plates were inverted and placed in a 37°C incubator overnight. Plates were then removed from the incubator and ϕX174 plaques were counted and recorded. A dilution series of the original virus suspension was also evaluated in a similar manner. Comparison of the results allowed estimation of the LRV (log reduction in viral load) as a result of the flocculant treatment. Results for several polymers are listed in the following Table 2: <tables id="tabl0003" num="0003"><table frame="topbot"><title>Table 2</title><tgroup cols="2"><colspec colnum="1" colname="col1" colwidth="61mm" /><colspec colnum="2" colname="col2" colwidth="23mm" colsep="0" /><thead><row><entry valign="top"><b>Polymer</b></entry><entry align="center" valign="top"><b>ϕX174 LRV</b></entry></row></thead><tbody><row><entry>Example 1 (0 NaCl)</entry><entry align="center">4.9</entry></row><row><entry>Example 1 (50mM NaCl)</entry><entry align="center">2.5</entry></row><row><entry>Example1 (150mM NaCl)</entry><entry align="center">2.9</entry></row><row><entry>Comparative Example 1 (0 NaCl)</entry><entry align="center">6.3</entry></row><row><entry>Comparative Example 1 (50mM NaCl)</entry><entry align="center">0.1</entry></row><row><entry>Comparative Example 1 (150mM NaCl)</entry><entry align="center">0.5</entry></row></tbody></tgroup></table></tables>

Example 13.

0085Poly(vinylmethylketone) (1.0 gram, from Scientific Polymer Products, Ontario, New York) was dissolved in 4 grams of ethyl acetate. To this solution was added methanol (5 grams), aminoguanidine hydrochloride (1.58 grams), and concentrated hydrochloric acid (1 drop). The mixture was mixed for 1 hour, followed by the addition of methanol (5 grams) and deionized water (5 grams) to produce an orange-colored solution. Infrared and NMR analysis confirmed the conversion to the guanylhydrazone polymer. The polymer was diluted to 1% solids by weight with deionized water, pH 7. for evaluation as a BSA precipitant. This polymer showed >50% BSA precipitation up to 250 mM NaCl concentration.

Comparative Example 2. Poly(diallyldimethylammonium chloride) (pDADMAC)

0086To a 1 liter, 3-necked round bottom flask equipped with an overhead stirrer, condenser, heating mantle, thermocouple, and nitrogen inlet was charged DADMAC (105.6 grams of a 65% solids aqueous solution, from Aldrich, Milwaukee, WI), tetrasodium ethylenediaminetetraacetic acid (6 µL of a 20% solution by weight in deionized water), deionized water (121.8 grams), and 2,2'-azobis(2-amidinopropane) dihydrochloride (1.74 grams). Stirring was begun and the mixture purged with a slow stream of nitrogen gas for 15 minutes. The temperature of the stirring mixture was then slowly raised to 60°C over one hour, then maintained at that temperature for an additional 24 hours. NMR analysis confirmed the formation of the expected polymer.

Examples 14-16.

0087Copolymers of DADMAC with diacetoneacrylamide by procedures similar to that described in Comparative Example 2. The copolymers, containing 20, 40, and 80% by weight diacetoneacrylamide, were reacted with aminoguanidine by the procedure described in Example 1, to produce the corresponding 20, 40, and 80% guanylhydrazone copolymers <u>14-16,</u><u>respectively</u>. Solutions of these copolymers and of pDADMAC were prepared at 1% by weight in deionized water (pH 7) and evaluated in the BSA precipitation test. These flocculants displayed 0%, 30%, 62%, and 92% BSA precipitation at 100 mM NaCl for Comparative Example 2, Ex. 14, Ex. 15, and Ex. 16, respectively.

Example 17.

0088A <i>Geobacillus stearothermophilus</i> purified spore suspension was provided by 3M consisting of approximately 1.4% by weight cell debris and spores. Test samples of broth containing 0, 100, 200, and 300 mM NaCl were prepared by a procedure similar to that described in Example 11. Solutions of polymers were prepared at 1.0% solids in DI water, pH 7, from the modified polymers of Examples 1, 6, and 8. A 1:4dilution series of each of these polymers was prepared to provide a total of 6 polymer concentrations. Then 2 mL of broth sample was mixed with 0.5 mL of polymer solution, and the mixture was tumbled for 30 minutes, then centrifuged at 200 rcf for 5 minutes. Standards were prepared by mixing 2 mL of broth with 0.5 mL of DI water, carrying the mixture through the same mixing/centrifugation procedure, then preparing a 2-fold serial dilution (6 samples) from the supernate. Supernatants from the test solutions and from the standards were pipetted into a 96-well microtitration plate and assayed by absorbance measurement at 650 nm. Comparison of the absorptions of the flocculant solutions to those of the standards provided a measure of the flocculation efficiencies. Results are presented in the following Table 3: <tables id="tabl0004" num="0004"><table frame="all"><title>Table 3</title><tgroup cols="7"><colspec colnum="1" colname="col1" colwidth="20mm" /><colspec colnum="2" colname="col2" colwidth="19mm" /><colspec colnum="3" colname="col3" colwidth="17mm" /><colspec colnum="4" colname="col4" colwidth="21mm" /><colspec colnum="5" colname="col5" colwidth="23mm" /><colspec colnum="6" colname="col6" colwidth="23mm" /><colspec colnum="7" colname="col7" colwidth="23mm" /><thead><row><entry valign="top" /><entry /><entry valign="top" /><entry namest="col4" nameend="col7" align="left" valign="top">% Turbidity Removed</entry></row><row><entry valign="top">Polymer</entry><entry valign="top">(w/v %)</entry><entry align="center">(Mg/ml)</entry><entry align="center">0 mM NaCl</entry><entry align="center">100 mM NaCl</entry><entry align="center">200 mM NaCl</entry><entry align="center">300 mM NaCl</entry></row></thead><tbody><row><entry>Example 1</entry><entry align="center" valign="bottom">0.2</entry><entry align="center" valign="bottom">2000</entry><entry align="center" valign="bottom">54.7</entry><entry align="center" valign="bottom">95.9</entry><entry align="center" valign="bottom">98.9</entry><entry align="center" valign="bottom">98.7</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.05</entry><entry align="center" valign="bottom">500</entry><entry align="center" valign="bottom">99.5</entry><entry align="center" valign="bottom">97.8</entry><entry align="center" valign="bottom">98.1</entry><entry align="center" valign="bottom">98.6</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.0125</entry><entry align="center" valign="bottom">125</entry><entry align="center" valign="bottom">95.7</entry><entry align="center" valign="bottom">97.0</entry><entry align="center" valign="bottom">95.0</entry><entry align="center" valign="bottom">92.1</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.003125</entry><entry align="center" valign="bottom">31.25</entry><entry align="center" valign="bottom">82.2</entry><entry align="center" valign="bottom">83.9</entry><entry align="center" valign="bottom">81.5</entry><entry align="center" valign="bottom">78.6</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.000781</entry><entry align="center" valign="bottom">7.81</entry><entry align="center" valign="bottom">76.6</entry><entry align="center" valign="bottom">73.5</entry><entry align="center" valign="bottom">71.5</entry><entry align="center" valign="bottom">68.0</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.000195</entry><entry align="center" valign="bottom">1.95</entry><entry align="center" valign="bottom">34</entry><entry align="center" valign="bottom">22.4</entry><entry align="center" valign="bottom">17.9</entry><entry align="center" valign="bottom">15.3</entry></row><row><entry>Example 6</entry><entry align="center" valign="bottom">0.2</entry><entry align="center" valign="bottom">2000</entry><entry align="center" valign="bottom">0.0</entry><entry align="center" valign="bottom">0.0</entry><entry align="center" valign="bottom">0.0</entry><entry align="center" valign="bottom">0.0</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.05</entry><entry align="center" valign="bottom">500</entry><entry align="center" valign="bottom">101.3</entry><entry align="center" valign="bottom">45.5</entry><entry align="center" valign="bottom">21.9</entry><entry align="center" valign="bottom">24.8</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.0125</entry><entry align="center" valign="bottom">125</entry><entry align="center" valign="bottom">96.8</entry><entry align="center" valign="bottom">97.7</entry><entry align="center" valign="bottom">97.8</entry><entry align="center" valign="bottom">98.5</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.003125</entry><entry align="center" valign="bottom">31.25</entry><entry align="center" valign="bottom">83.5</entry><entry align="center" valign="bottom">82.1</entry><entry align="center" valign="bottom">81.6</entry><entry align="center" valign="bottom">78.5</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.000781</entry><entry align="center" valign="bottom">7.81</entry><entry align="center" valign="bottom">70.9</entry><entry align="center" valign="bottom">76.5</entry><entry align="center" valign="bottom">66.4</entry><entry align="center" valign="bottom">52.2</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.000195</entry><entry align="center" valign="bottom">1.95</entry><entry align="center" valign="bottom">25.8</entry><entry align="center" valign="bottom">28</entry><entry align="center" valign="bottom">24.4</entry><entry align="center" valign="bottom">17.5</entry></row><row><entry>Example 8</entry><entry align="center" valign="bottom">0.2</entry><entry align="center" valign="bottom">2000</entry><entry align="center" valign="bottom">0.0</entry><entry align="center" valign="bottom">0.0</entry><entry align="center" valign="bottom">0.0</entry><entry align="center" valign="bottom">0.0</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.05</entry><entry align="center" valign="bottom">500</entry><entry align="center" valign="bottom">53.4</entry><entry align="center" valign="bottom">42.2</entry><entry align="center" valign="bottom">9.8</entry><entry align="center" valign="bottom">10.5</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.0125</entry><entry align="center" valign="bottom">125</entry><entry align="center" valign="bottom">90.0</entry><entry align="center" valign="bottom">74.2</entry><entry align="center" valign="bottom">14.6</entry><entry align="center" valign="bottom">37.9</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.003125</entry><entry align="center" valign="bottom">31.25</entry><entry align="center" valign="bottom">98.6</entry><entry align="center" valign="bottom">88.5</entry><entry align="center" valign="bottom">58.7</entry><entry align="center" valign="bottom">55.1</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.000781</entry><entry align="center" valign="bottom">7.81</entry><entry align="center" valign="bottom">80.0</entry><entry align="center" valign="bottom">75.1</entry><entry align="center" valign="bottom">67.7</entry><entry align="center" valign="bottom">56.3</entry></row><row><entry>"</entry><entry align="center" valign="bottom">0.000195</entry><entry align="center" valign="bottom">1.95</entry><entry align="center" valign="bottom">70.4</entry><entry align="center" valign="bottom">68.2</entry><entry align="center" valign="bottom">64.5</entry><entry align="center" valign="bottom">40.8</entry></row></tbody></tgroup></table></tables>

Examples 18-20 and Comparative Examples 3-4

0089A 20:80 w/w copolymer of N,N-dimethylaminopropylacrylamide (DMAPAm) and diacetoneacrylamide (Comparative Example 3) was prepared in methanol solution by the procedure described in Example 1.

0090A sample of this polymer solution was diluted to 20% solids by adding more methanol, and enough butyl bromide was added to alkylate 50% of the DMAPAm units of the copolymer (Comparative Example 4).

0091Samples of Comparative Example 3 polymer solution were reacted with aminoguanidine hydrochloride by a procedure similar to that of Example 6 to produce copolymers in which 50% and 100% of the diacetoneacrylamide units were converted to guanylhydrazone units, respectively (Examples 18 and 19).

0092A sample of Comparative Example 4 was reacted with aminoguanidine hydrochloride by a procedure similar to that of Example 6 to convert all of the diacetoneacrylamide units to guanylhydrazone units (Example 20).

0093Samples of each of the above examples were diluted to 1% solids with deionized water, adjusted to pH 7, and evaluated for BSA precipitation (Table 4) <tables id="tabl0005" num="0005"><table frame="all"><title>Table 4</title><tgroup cols="6"><colspec colnum="1" colname="col1" colwidth="15mm" /><colspec colnum="2" colname="col2" colwidth="17mm" /><colspec colnum="3" colname="col3" colwidth="17mm" /><colspec colnum="4" colname="col4" colwidth="14mm" /><colspec colnum="5" colname="col5" colwidth="14mm" /><colspec colnum="6" colname="col6" colwidth="14mm" /><thead><row><entry align="center" valign="top">[NaCl]</entry><entry namest="col2" nameend="col6" align="center" valign="top">% BSA Precipitated</entry></row><row><entry align="center" valign="top">(mM)</entry><entry align="center" valign="top">Comp. 3</entry><entry align="center" valign="top">Comp. 4</entry><entry align="center" valign="top">Ex. 18</entry><entry align="center" valign="top">Ex. 19</entry><entry align="center" valign="top">Ex.20</entry></row></thead><tbody><row rowsep="0"><entry align="center">50</entry><entry align="center">0</entry><entry align="center">0</entry><entry align="center">62</entry><entry align="center">89</entry><entry align="center">93</entry></row><row rowsep="0"><entry align="center">150</entry><entry align="center">0</entry><entry align="center">0</entry><entry align="center">28</entry><entry align="center">47</entry><entry align="center">86</entry></row><row><entry align="center">250</entry><entry align="center">0</entry><entry align="center">1</entry><entry align="center">14</entry><entry align="center">18</entry><entry align="center">75</entry></row></tbody></tgroup></table></tables>

0094Similar copolymers were prepared by alkylation with dimethylsulfate, benzyl bromide and dodecylbromide, respectively, followed by conversion of the keto groups to guanylhydrazones. When tested for BSA precipitation, a synergistic effect was again observed for alkylation plus guanylation.

Example 21.

0095A 50:50 w/w copolymer of DADMAC with diacetoneacrylamide was prepared by a procedure similar to that described in Comparative Example 2. A solution of this copolymer in methanol (1.32 grams of 30% solids solution) was diluted to 2% solids with deionized water, mixed with diaminoguanidine hydrochloride (74 milligrams) and concentrated hydrochloric acid (1 drop), and allowed to react at room temperature overnight to form the bis-guanylhydrazone copolymer. The solution was adjusted to pH 7 and diluted to 1% solids with DI water. This polymer exhibited good flocculation of BSA in solutions containing up to 250 mM sodium chloride.

Example 22.

0096Diacetoneacrylamide (33.8 grams), methanol (400 mL), and aminoguanidine hydrochloride (22.1 grams) were placed in a 1 L round bottom flask and stirred magnetically until a homogeneous solution had formed. Trifluoroacetic acid (0.5 mL) was added and the mixture was stirred overnight at ambient temperature. The reaction mixture was concentrated on a rotary evaporator to produce a light yellow oil which crystallized on standing. Diethyl ether (250 mL) was added and the crystalline mass was broken up, filtered, washed with additional ether, and dried under vacuum for 3 hours to give the corresponding guanylhydrazone hydrochloride monomer (53.5 grams, 95.7% yield). This monomer (10 grams) was mixed with deionized water (23 grams), methanol (7 grams), and 2,2'-azobis(2-imidazolin-2-yl)propane (0.2 grams, from WAKO, Osaka, Japan). The mixture was purged with nitrogen gas for 15 minutes, then polymerized at 60°C for 24 hours as described in Example 1. This polymer displayed flocculation properties identical to those of Example 1.

Reference Example 23.

0097A polypropylene spun bond/melt blown/spun bond (SMS) nonwoven (50 gsm basis weight) was coated with the polymer of Example 1 in a layer-by-layer process. Three coating baths were prepared: <tables id="tabl0006" num="0006"><table frame="none"><tgroup cols="2" colsep="0" rowsep="0"><colspec colnum="1" colname="col1" colwidth="29mm" /><colspec colnum="2" colname="col2" colwidth="135mm" /><tbody><row><entry>Coating Bath #1:</entry><entry>1% wt/wt polyethylenimine (70,000 M_n) in isopropyl alcohol;</entry></row><row><entry>Coating Bath #2:</entry><entry>0.5% wt/wt poly(2-acrylamido-2-methyl-1-propanesulfonic acid, sodium salt) in deionized water;</entry></row><row><entry>Coating Bath #3:</entry><entry>1% wt/wt polymer of Example 1 in deionized water.</entry></row></tbody></tgroup></table></tables> The following coating sequence was conducted (5 minute residence time in each bath) with a 5 minute rinse with deionized water after each coating layer was applied: #1, #2, #1, #2, #3, #2, #3, #2, and #3. After drying at room temperature overnight, a 16 mm diameter disk was cut from the coated web. This disk was placed in a 5 mL centrifuge tube, 2 mL of a <i>Clostridium sporogenes</i> spore suspension (with and without added sodium chloride) was added, and the mixture tumbled at room temperature for 30 minutes. The UV absorbance of the supernatant solution was measured at 640 nm and compared to the absorbance prior to contact with the disk, and indicated 46% and 58% removal of spores at 0 and 250 mM sodium chloride concentrations, respectively.

Reference Example 24.

0098Aminosilane coated glass microscope slides (obtained from Newcomer Supply, Middleton,WI) were dip coated, in sequence, with coating solution #2 from Example 23, rinsed with deionized water, coating solution #3 from Example 23, rinsed with deionized water, and dried at room temperature. The coated slides could then be used to capture bacteria or spores from aqueous media for enumeration or for identification. A control aminosilane slide coated with only coating solution #2 was found to bind almost no bacteria or spores.

Reference Examples 25-28.

0099A diacetoneacrylamide solution was prepared at 10% by weight in isopropanol. Several sheet substrates (ca. 10.16 cm by 15.24 cm (4 inches by 6 inches)), both membrane and non-woven, as listed in the table below, were placed in polyethylene bags (Ziploc™, S C Johnson, Racine, WI). Using a disposable pipette, the substrates were completely wetted with the diacetoneacrylamide solution. The excess solution was removed by zipping the bags shut and running over the bags with a rubber roller. The pooled excess solutions were removed with a paper towel. The bags were then vacuum-packed into Foodsaver™ bags. The bags were deliberately not purged of oxygen so as to minimize solvent evaporation. The samples were placed in an aluminum tote and exposed for 1 hour to Co-60 y radiation. The dose exposure corresponded to 5 kGy. The samples were taken out of the bags and washed with copious amounts of water to remove any traces of homopolymer. The completely washed samples were then air dried.

0100The diacetoneacrylamide grafted membranes were then converted to guanylhydrazone functional membranes. An aminoguanidine solution was prepared by dissolving 65 grams in 1 liter of methanol, and adding trifluoroacetic acid (2.25 mL). Each membrane was placed in a 500 mL polyethylene bottle, 100 mL of the aminoguanidine solution was added, the bottles were sealed, and the mixtures were placed on a roller at room temperature overnight. Excess solution was removed, and the membranes were washed extensively with deionized water and dried.

010124 mm disks were punched out of the sheets and placed in a centrifuge tube. Bovine serum albumin solution (BSA, Sigma Aldrich) was prepared to a concentration of 0.75 mg/ml in 25 mM TRIS buffer, pH 8.0 (tris(hydroxymethyl)aminomethane, Sigma). 4.5 ml of the BSA solution was pipetted into each centrifuge tube, the tubes were capped, and the tubes were tumbled overnight. The supernatant solutions were analyzed by a UV-VIS spectrometer at 279 nm with background correction applied at 325 nm. Static binding capacities for the samples were found to be between 1 and 4 mg/mL. Analyses of the ungrafted substrates displayed binding capacities of &lt; 0.2 mg/mL. <tables id="tabl0007" num="0007"><table frame="all"><tgroup cols="2"><colspec colnum="1" colname="col1" colwidth="24mm" /><colspec colnum="2" colname="col2" colwidth="140mm" /><thead><row><entry valign="top"><b><u>Table</u></b></entry><entry align="center" valign="top"><b>Substrate</b></entry></row></thead><tbody><row><entry valign="bottom">Example 25</entry><entry valign="bottom">Polyvinylidene fluoride membrane, 2 µm nominal pore size</entry></row><row><entry valign="bottom">Example 26</entry><entry valign="bottom">Nylon 6 melt-blown non-woven web</entry></row><row><entry valign="bottom">Example 27</entry><entry valign="bottom">Nylon 66 membrane, single reinforced layer nylon three zone membrane, nominal pore size 1.8 µm</entry></row><row><entry valign="bottom">Example 28</entry><entry valign="bottom">Polypropylene non-woven web, sold as 3M Doodleduster ™ cloth</entry></row></tbody></tgroup></table></tables>

Reference Example 29

0102A 60 gsm nylon 6 meltblown nonwoven substrate with a 4.3 um effective fiber diameter and sample size of about 30 cm X 43 cm (1290 cm² or 200 square inches) was purged of air and inserted into Ziploc bags in a glove box under nitrogen atmosphere (less than 20 ppm oxygen) and sealed. The Ziploc bag was then removed from the glove box, and irradiated to a dose level of 60 kGy (kilogray) by passing it through the electron beam. The bag was returned to the N₂ atmosphere-controlled glove box.

0103The Ziplock bag was opened and the nonwoven substrate was imbibed by pouring 150 grams of a nitrogen purged imbibing solution comprising; 17% diacetone acrylamide monomer, 10% polyethylene glycol with hydroxy end groups, average molecular weight 4,600 g/mole (PEG 4600), 1.0% Sartomer SR-550 PEG-acrylate and 72% water by weight inside the Ziploc bag with the nonwoven substrate. The nonwoven is kept flat and is completely and evenly saturated with a slight excess of grafting solution. The Ziploc bag is sealed. The Ziploc bag remains inside of the nitrogen filled glove box to allow sufficient time for all of the grafting monomer to graft-polymerize by free radical reaction with the nonwoven substrate. After a minimum of 6 hrs or as long as about 18 hrs (overnight), the grafted nylon nonwoven sample was removed from the glovebox and the Ziploc bag and the grafted nonwoven was washed three times by soaking in 2 liters of fresh DI water (to remove the PEG and any unreacted monomer) and then air dried to ambient conditions.

0104A solution of aminoguanidine (92 grams) and concentrated hydrochloric acid (3.45 mL) in deionized water (1500 mL total solution volume) was prepared. A sheet of the grafted nonwoven (ca. 12 cm x 40 cm) was placed in a 60.6 liter (1 gallon) plastic jar. A portion (500 mL) of aminoguanidine solution was added to the jar, which was then sealed and placed on a roller for overnight reaction at room temperature. The excess aminoguanidine solution was decanted, the derivatized nonwoven sheet was rinsed under a stream or deionized water for 15 minutes, and the sheet then allowed to air dry at room temperature. A 24 mm diameter disk was cut from the coated web. This disk was placed in a 5 mL centrifuge tube, 4.5 mL of a Bovine serum albumin (BSA) solution (ca. 3 mg/mL in 25 mM TRIS, pH 8.0) was added, and the mixture tumbled at room temperature overnight. The UV absorbance of the supernatant solution was measured at 279 nm and compared to the absorbance prior to contact with the disk to determine the static binding capacity for BSA. The functionalized nonwoven was found to absorb 152 mg of BSA per gram of web.

Example 30.

0105Using standard microbiological procedures, cultures of the following were prepared: <ol id="ol0001" compact="compact" ol-style=""><li>a) <i>Bacillus atrophaeous</i> (spores and cell debris)</li><li>b) <i>Clostridium sporogenes</i> (spores)</li><li>c) <i>Escherichia coli</i> (cells and cell debris)</li><li>d) Chinese hamster ovary (CHO) cells</li><li>e) Baker's yeast</li></ol>

0106When flocculation experiments were conducted similarly to those described in Example 17 on these mixtures, the ligand functional polymers of the invention consistently displayed good flocculating ability in the presence of sodium chloride concentrations in excess of 50 mM.

Example 31.

0107Aminoguanidine hydrochloride (1.11 grams), methanol (15 grams), and acetoacetoxyethyl methacrylate (2.2 grams) were stirred at room temperature and monitored periodically by NMR. After stirring for 21 hours, NMR confirmed complete conversion of the methacrylate monomer to the corresponding guanylhydrazone hydrochloride