Nova Patents
EP2876166B1

New compound for sequencing by synthesis

Abstract

This record has no abstract on file.

EP2876166B1, drawing sheet 1
Sheet 1 of 3

Term

7.2 yearsleft in the term

Expires 20 November 2033.

  1. Priority and filed
  2. Granted
  3. Today
  4. Expires

13 claims: 6 independent, 7 dependent

  1. 1
    Use of a deoxynucleoside tri- or tetraphosphate comprising a 3'-nitrate and a detectable label covalently bound to the oxygen atom of a oxymethyl or oxyallyl or oxypropargyl substitution of a nucleobase within a polymerase catalyzed DNA synthesis reaction, wherein said DNA synthesis reaction is performed in the presence of pyrophosphatase.
  2. 4
    A kit comprising - a DNA polymerase, - a pyrophosphatase, and - at least one deoxynucleoside tri- or tetraphosphate comprising a 3'-nitrate and a detectable label covalently bound to the oxygen atom of a oxymethyl or oxyallyl or oxypropargyl substitution of a nucleobase
  3. 5
    A reaction mixture comprising - a nucleic acid template with a primer hybridized to said template, - a DNA polymerase, - a pyrophosphatase, and - at least one deoxynucleoside tri- or tetraphosphate comprising a 3'-nitrate and a detectable label covalently bound to the oxygen atom of a oxymethyl or oxyallyl or oxypropargyl substitution of a nucleobase.
  4. 6
    Use of pyrophosphatase for stabilizing deoxynucleotide 3'-nitrate ester during and subsequent to a polymerase catalyzed DNA synthesis reaction.
  5. 7
    A method of performing a DNA synthesis reaction comprising the steps of - preparing a reaction mixture by means of providing a nucleic acid template with a primer hybridized to said template, a DNA polymerase, at least one deoxynucleoside tri- or tetraphosphate comprising a 3'-nitrate and a detectable label covalently bound to the oxygen atom of a oxymethyl or oxyallyl or oxypropargyl substitution of a nucleobase, a pyrophosphatase, and - subjecting said reaction mixture to conditions which enable a DNA polymerase catalyzed primer extension reaction.
  6. 9
    A method for analyzing a DNA sequence comprising the steps of a) providing a nucleic acid template with a primer hybridized to said template forming a primer/template hybridization complex, b) adding DNA polymerase, and at least one labeled deoxynucleoside tri- or tetraphosphate comprising a 3'-nitrate and a detectable label covalently bound to the oxygen atom of a oxymethyl or oxyallyl or oxypropargyl substitution of a nucleobase, and a pyrophosphatase, c) subjecting said reaction mixture to conditions which enable a DNA polymerase catalyzed primer extension reaction, and d) determining, whether said compound has been incorporated into said primer/template hybridization complex.
Independent claims6