Magnolia extract containing compositions

Description of equivalent WO 2008130752 A2

DESCRIPTION

MAGNOLIA EXTRACT CONTAINING COMPOSITIONS

CROSS-REFERENCE TO RELATED APPLICATIONS

0004[0001] This application claims the benefit of U.S. Provisional Application Serial No.

000560/912,793, filed April 19, 2007, the contents of which are incorporated by reference.

BACKGROUND OF THE INVENTION

0007A. Field of the Invention

0008[0002] The present invention relates generally to skin care compositions. In non- limiting aspects, the compositions can be used to treat skin conditions such as telangiectasia, eye circles, and puffy eyes. In certain embodiments, the compositions can include a Magnolia extract and can be incorporated into cosmetic products.

0009B. Background of the Invention

0010[0003] Ageing, chronic exposure to adverse environmental factors, malnutrition, fatigue, etc., can change the visual appearance, physical properties, or physiological functions of skin in ways that are considered visually undesirable. Some notable changes include the appearance spider veins, eye circles (e.g. , dark circles under the eye), and puffy eyes. Other changes include the development of aged or environmentally damaged skin which can include the appearance fine lines and wrinkles, loss of elasticity, increased sagging, loss of firmness, loss of color evenness or tone, coarse surface texture, and mottled pigmentation. Less obvious, but measurable changes which occur as skin ages or endures chronic environmental insult include a general reduction in cellular and tissue vitality, reduction in cell replication rates, reduced cutaneous blood flow, reduced moisture content, accumulated errors in structure and function, alterations in the normal regulation of common biochemical pathways, and a reduction in the skin's ability to remodel and repair itself. Many of the alterations in appearance and function of the skin are caused by changes in the outer epidermal layer of the skin, while others are caused by changes in the lower dermis. 1. Spider Veins

0011[0004] Spider veins (i.e., telangiectasias or sunburst varicosities) are formed by the dilation of a small group of blood vessels located close to the surface of the skin. Although they can appear anywhere on the body, spider veins are most commonly found on the face and legs. They can be visible to the naked eye and typically appear as unsightly clusters of red, blue or purple veins on the thighs, calves, and ankles of people. It is estimated that over half of the adult female population suffers from this cosmetic problem. Factors that can contribute to the development of spider veins include heredity, pregnancy, and other events that cause hormonal shifts, weight gain, occupations or activities that require prolonged sitting or standing, and the use of certain medications.

0012[0005] Typical methods for treating spider veins is through cosmetic surgery (e.g., sclerotherapy, laser surgery, electrodesiccation, surgical ligation, and ambulatory phlebectomy). For instance, sclerotherapy is a surgical procedure where veins are injected with a sclerosing solution, which causes them to collapse and fade from view. Risks associated with sclerotherapy include the formation of blood clots in the veins, severe inflammation, adverse allergic reactions to the sclerosing solution, and skin injury that can lead to permanent scarring. Further, it is common to develop irregular skin pigmentation in the treated areas (e.g., brownish splotches) that can take several months to fade. Another problem associated with sclerotherapy is "telangiectatic matting," where fine reddish blood vessels appear around the treated area, requiring further injections. Other surgical methods can have similar side effects.

00132. Eye Circles and Puffy Eyes

0014[0006] The skin around the periorbital area (i.e., around the eyes) is thin and delicate.

0015Like all skin, the periorbital area is webbed with tiny capillaries. Blood sometimes leaks from these capillaries which can cause the appearance of dark circles under the eye. Other known causes dark under eye circles include UV exposure (e.g., exposure to the sun can increase natural melanin levels and draws the melanin to the surface of the skin, making it darker), ageing (e.g., with age, the skin around the eyes can become even thinner which makes dark under eye circles become more pronounced), fatigue (being tired can make skin paler which makes dark circles look darker), allergies (e.g., allergic reactions can cause smudges in the under eye area and conditions that causes a person to rub their eyes can make dark circles worse because scratching or rubbing can darken the skin), pregnancy or menstruation (e.g., skin becomes pale during pregnancy and menstruation which makes dark circles look darker), and inadequate nutrition (e.g., lack of key nutrients such as iron can cause dark under eye circles).

0016[0007] One method for treating under eye circles includes topical application of a composition having hydroquinone. Hydroquinone, however, can be toxic, and it may actually cause hyper-pigmentation and make the dark circles darker. Cosmetic concealers can be used to hide the dark circles. Unfortunately, the dark circles become visible again once the concealer is removed. Chamomile has also been used, but can cause allergic reactions.

0017[0008] As for puffy eyes, this is a condition where the skin under the eyes swells which can be visually undesirable. Puffy eyes can be caused by several factors including increased vascularization, leaky capillaries, thinning/slackening skin which can fill up with more fluid, loss of the fat pad under the eye which can contribute to under eye bags, and allergies, dusts, and pollutants which can trigger a release of chemicals thereby swelling the tissue around the eyes.

0018[0009] One method of treating puffy eyes includes washing the face with cold water to reduce swelling. Other treatments include dietary restrictions (e.g. , limiting the intake of salt), placing slices of cucumbers on the eyes, or placing tea bags in cold water and subsequently placing the bags on the eyes. These treatment options can be limiting in that the effects can oftentimes be negligible or short-lived.

00193. Aged or Environmentally Damaged Skin

0020[0010] Several different approaches have been used to treat damaged skin caused by aging, environmental factors, chemicals, or malnutrition. One approach involves the use of specific agents to directly stimulate or inhibit selected biochemical targets. Examples include the use of retinoids to stimulate collagen and glycosaminoglycan synthesis by fibroblasts (Schiltz, et al, 1986). Another approach is to use agents or processes that stimulate the rate at which the epidermis replaces itself, a process known as epidermal cell renewal. Increases in epidermal cell renewal rates usually result from a more rapid rate of replication of epidermal basal cells, and can be caused by diverse stimuli such as chemical or physical injury, adverse environmental conditions, or direct stimulators of basal cell division. [0011] Several of the above methods have been shown to have various drawbacks, such as significant irritation to the skin or skin toxicity. In addition, most of these methods involve the invocation of chronic damage to the skin, which sets up repair mechanisms. For most of the existing treatments, there will be a period of time, up to several weeks or months, during which the skin becomes irritated and after which tolerance sets in and the symptoms of irritation may decrease and/or cease.

SUMMARY OF THE INVENTION

0022[0012] The present invention provides compositions and methods that can be used to treat the appearance of a person's skin.

0023[0013] In one embodiment, there is disclosed a topical skin care composition that includes, consists essentially of, or consists of at least one of the following ingredients: Magnolia extract; honokiol, magnolol, humulus lupulus extract, hesperidin methyl chalcone, gotu kola (centella asiatica extract), dipeptide valyl -tryptophane, palmitoyl tetrapeptide-3, corylus avellana bud extract, cucumis sativa extract, morus alba extract, hibiscus sabdariffa flower extract, vitis vinifera extract, ascorbyl glucoside, citrus medica limonum extract, avena sativa kernel extract, hydrolyzed soy protein, aniseed myrtle extract, tasmania lanceolata leaf extract, artemisia abrotanum extract, citrus grandis fruit extract, or apigenin. These ingredients can be isolated or purified prior to their inclusion in a corresponding composition. It is contemplated that compositions of the present invention can include at least 2, 3, 4, 5, 6, 7, 8, 9, or more of these ingredients in any different combination. The combination of ingredients can be formulated into blends and subsequently added to the composition. Non- limiting examples of Magnolia species from which the Magnolia extract can be obtained include Magnolia acuminata, Magnolia ashei, Magnolia biondii, Magnolia cylindrica, Magnolia cambellii, Magnolia denudata, Magnolia fraseri, Magnolia grandiflora, Magnolia hypoleuca, Magnolia kobus, Magnolia hliiflora, Magnolia loegneri, Magnolia macrophylla, Magnolia officinalis, Magnolia pyramidata, Magnolia sargentiana, Magnolia seiboldii, Magnolia soulangiana, Magnolia sprengeri, Magnolia stellata, Magnolia tripetala, Magnolia virginiana, Magnolia zenii, and Michelia figo. In certain embodiments, the composition can include a Magnolia extract and isolated or purified honokiol or magnolol or a combination of all three. The Magnolia extract in particular embodiments is Magnolia biondii extract. In other aspects, the composition can include Magnolia extract and humulus lupulus extract, Magnolia extract and hesperidin methyl chalcone and gotu kola, Magnolia extract and dipeptide valyl-tryptophane, Magnolia extract and palmitoyl tetrapeptide-3, Magnolia extract and corylus avellana bud extract, Magnolia extract and a botanical blend comprising cucumis sativa extract, morus alba extract, hibiscus sabdariffa flower extract, and vitis vinifera extract, Magnolia extract and ascorbyl glucoside, citrus medica limonum extract, and cucumis sativa extract, Magnolia extract and a blend of avena sativa kernel extract, hydrolyzed soy protein, aniseed myrtle extract, tasmania lanceolata leaf extract, and hibiscus sabdariffa flower extract, Magnolia extract and artemisia abrotanum extract, Magnolia extract and citrus grandis fruit extract, or Magnolia extract and isolated or purified apigenin. In certain aspects, the citrus grandis fruit extract includes at least 90% by weight of apigenin. The compositions can also include a cooling agent (e.g., an agent that provides a cooling sensation when applied to the skin. The cooling agent can be those known in the art such as, for example, menthol or menthol derivatives (e.g., menthyl lactate and menthone glycerin acetal).

0024[0014] In certain aspects, the compositions can be formulated to have a pH of about less than 4.0, or 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6,

00255.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7,

00267.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8,

00279.9, 10.0, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11.0, 11.1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9, to about 12.0, or more, or any range or integer derivable therein. In other aspects, the compositions can be formulated into a cosmetic product (e.g., skin cleanser, moisturizer, concealer, etc.). The composition can be comprised in a cosmetic vehicle (e.g., an emulsion, cream, lotion, solution, anhydrous base, gel, ointment, etc.). The composition can be in a dry, powdered, liquid, solid, semi-solid, spray, or aerosol form. It is contemplated that the compositions of the present invention can be used in combination with other cosmetic products (e.g., a composition of the present invention can be formulated into a concealer product which can be used in conjunction with a foundation product). The compositions can be formulated for application to skin at least 1, 2, 3, 4, 5, 6, 7, or more times per day.

0028[0015] As described throughout this specification, the ingredients in compositions of the present invention can be present within the compositions in a variety of amounts. The amounts can be measured by total weight or volume of the composition. By way of example only, an ingredient can be included into the composition at 0.0001, 0.001, 0.01, 0.1, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98, 99%, or more, or any range or integer derivable therein, by weight or volume of the total composition. In certain aspects, Magnolia extract can be in a composition from about 0.1% to about 10.0% by weight. The ratio of any ingredient within the composition when compared to another ingredient can be from about 1 :1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31 :1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, 100:1, or more or any number derivable therein, by weight or volume of the total composition, hi other aspects, the ratio of any ingredient within the composition when compared to another ingredient can be from about 1 :2, 1 :3, 1 :4, 1 :5, 1 :6, 1 :7, 1 :8, 1 :9, 1 :10, 1 :11, 1:12, 1 :13, 1 :14, 1 :15, 1 :16, 1 :17, 1 :18, 1 :19, 1 :20, 1 :21, 1 :22, 1 :23, 1 :24, 1 :25, 1:26, 1 :27, 1 :28, 1 :29, 1:30, 1:31, 1:32, 1:33, 1:34, 1:35, 1:36, 1 :37, 1:38, 1 :39, 1:40, 1 :50, 1 :60, 1 :70, 1 :80, 1:90, 1 :100, or more or any number derivable therein, by weight or volume of the total composition.

0029[0016] It is also contemplated that the viscosity of the composition can be selected to achieve a desired result (e.g., depending on the type of composition desired, the viscosity of such composition can be from about 1 cps to well over 1 million cps or any range or integer derivable therein (e.g., 2 cps, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000, 60000, 70000, 80000, 90000, 100000, 200000, 300000, 400000, 500000, 600000, 700000, 800000, 900000, 1000000 cps, etc.).

0030[0017] The compositions of the present invention can include additional ingredients that can be included into cosmetic or pharmaceutical compositions. As explained throughout this specification, non-limiting examples of additional ingredients can include essential oils, volatile, and non-volatile oils, thickening agents, surfactants, preservatives, silicone containing compounds, absorbents, adsorbents, chelating agents, lubricants, solvents, moisturizers (including, e.g., emollients, humectants, film formers, occlusive agents, and agents that affect the natural moisturization mechanisms of the skin), water repellents, antioxidants, UV absorbers, anti-irritants, anti-microbial agents, dyes and color ingredients, or structuring agents, or any combination thereof.

0031[0018] In other aspects of the present invention, there is disclosed a method of treating a skin condition, comprising topically applying an effective amount of a composition of the present invention to skin. Topical application of the composition can treat or prevent such a skin condition. The effectiveness of the composition can be compared with skin that has not been treated with a composition of the present invention. In certain non-limiting embodiments, the skin treatment can be localized to and/or around an area where the composition is applied to the skin. The skin can be facial, torso, back, neck, ear, pelvic, arms, hands, legs (e.g., ankle, knee, thigh), feet, or buttocks skin. For instance, topical application of a composition to , wherein topical application of the composition treats the skin condition. Non-limiting examples of skin conditions that can be treated or prevented with compositions of the present invention include telangiectasia (i.e., spider veins), eye circles (e.g., dark circles under the eye), puffy eyes, pruritus, lentigo, age spots, senile purpura, keratosis, melasma, blotches, wrinkles, fine lines, nodules, sun damaged skin, dermatitis (including, but not limited to seborrheic dermatitis, nummular dermatitis, contact dermatitis, atopic dermatitis, exfoliative dermatitis, perioral dermatitis, and stasis dermatitis), psoriasis, folliculitis, rosacea, acne, impetigo, erysipelas, erythrasma, eczema, or hyperpigmentation. In certain aspects, the skin condition can be caused by exposure to UV light, age, irradiation, chronic sun exposure, environmental pollutants, air pollution, wind, cold, heat, chemicals, disease pathologies, or smoking. The skin to be treated can be aged, nutritionally compromised, or environmentally damaged skin. In certain aspects, the composition can be topically applied in an amount effective to increase the stratum corneum turnover rate of the skin, collagen synthesis production of the skin, fat production of the skin, firmness of the skin, or elasticity of the skin. In other aspects, the composition can be topically applied in an amount effective to reduce or inhibit new capillary formation in or near the skin, blood flow to the skin, fluid amount in or near the skin, or melanin production in the skin.

0032[0019] Also disclosed are kits that can include a composition of the present invention.

0033In certain non-limiting aspects, the composition is comprised in a container. The container can be a bottle, dispenser, package, etc. The container can be configured to dispense a predetermined amount of the composition. The container can be configured to dispense the composition in a semi-solid, liquid, spray, or an aerosol form. In certain aspects, the kit can include indicial on its surface and/or instructions for using the composition.

0034[0020] In other aspects of the present invention, the composition can be used as part of a regimen to treat a skin condition. For instance, the regimen can include applying a composition of the present invention in a first instance as disclosed throughout this specification. The regimen can then include additional applications that are identical, similar, or different than the first instance application. The additional applications can include, for example, a second, third, fourth, fifth, sixth, seventh, eighth, nine, tenth, or more applications with a composition of the present invention and/or whether another method for treating a particular skin condition (e.g., other compositions, surgeries, etc.).

0035[0021] It is contemplated that any embodiment discussed in this specification can be implemented with respect to any method or composition of the invention, and vice versa. Furthermore, compositions of the invention can be used to achieve methods of the invention.

0036[0022] The terms "inhibiting," "reducing," or "prevention," or any variation of these terms, when used in the claims and/or the specification includes any measurable decrease or complete inhibition to achieve a desired result.

0037[0023] The term "effective," as that term is used in the specification and/or claims, means adequate to accomplish a desired, expected, or intended result.

0038[0024] The use of the word "a" or "an" when used in conjunction with the term

0039"comprising" in the claims and/or the specification may mean "one," but it is also consistent with the meaning of "one or more," "at least one," and "one or more than one."

0040[0025] The terms "about" or "approximately" are defined as being close to as understood by one of ordinary skill in the art, and in one non-limiting embodiment the terms are defined to be within 10%, within 5%, within 1%, and in certain aspects within 0.5%.

0041[0026] The use of the term "or" in the claims is used to mean "and/or" unless explicitly indicated to refer to alternatives only or the alternatives are mutually exclusive, although the disclosure supports a definition that refers to only alternatives and "and/or."

0042[0027] As used in this specification and claim(s), the words "comprising" (and any form of comprising, such as "comprise" and "comprises"), "having" (and any form of having, such as "have" and "has"), "including" (and any form of including, such as "includes" and "include") or "containing" (and any form of containing, such as "contains" and "contain") are inclusive or open-ended and do not exclude additional, unrecited elements or method steps. [0028] Other objects, features and advantages of the present invention will become apparent from the following detailed description. It should be understood, however, that the detailed description and the examples, while indicating specific embodiments of the invention, are given by way of illustration only. Additionally, it is contemplated that changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.

DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

0044[0029] In today's image conscious society, people are continually looking for a product that can improve the visual appearance of their skin. The skin's appearance can be affected in a negative way from skin conditions. For instance, spider veins (i.e., telangiectasias or sunburst varicosities) can appear on a person's skin {e.g., face, thighs, calves, ankles, arms, torso, buttocks, etc.) as unsightly clusters of red, blue or purple veins. Under eye circles and puffy eyes can appear as dark circles and swelling around the periorbital area, respectively. Additionally, aged or environmentally damaged skin which can include the appearance fine lines and wrinkles, loss of elasticity, increased sagging, loss of firmness, loss of color evenness or tone, coarse surface texture, and mottled pigmentation.

0045[0030] In one aspect, the present invention provides compositions and methods for treating skin conditions. In one non-limiting embodiment, the compositions of the present invention can be used to treat spider veins, eye circles, puffy eyes, or environmentally damaged skin by topically applying a composition of the present invention to an area of the skin that needs such treatment. As noted throughout this specification, the compositions can include at least one or any combination of the following ingredients: Magnolia extract; honokiol, magnolol, humulus lupulus extract, hesperidin methyl chalcone, gotu kola, dipeptide valyl -tryptophane, palmitoyl tetrapeptide-3, corylus avellana bud extract, cucumis sativa extract, morus alba extract, hibiscus sabdariffa flower extract, vitis vinifera extract, ascorbyl glucoside, citrus medica limonum extract, avena sativa kernel extract, hydrolyzed soy protein, aniseed myrtle extract, tasmania lanceolata leaf extract, artemisia abrotanum extract, citrus grandis fruit extract, or apigenin. These and other non-limiting aspects of the present invention are described in further detail in the following sections. A. Ingredients

0046[0031] The following subsections provide non-limiting examples of ingredients that can be included into compositions of the present invention. The compositions can include any one of the following ingredients or a combination of such ingredients. It is contemplated that other ingredients can also be incorporated into the compositions. Further, a person of ordinary skill in the art would recognize that the ingredients are commercially available, can be chemically synthesized, or can be isolated or purified by known methods from sources that includes such ingredients.

00471. Magnolia Extract

0048[0032] In certain non-limiting embodiments, the compositions of the present invention can include a Magnolia extract. The Magnolia extract can be obtained or derived from a variety of sources from a Magnolia plant (e.g., flower, bark, seed cone, etc.). In general, Magnolia is a large genus of about 210 flowering plant species in the subfamily Magnoliodieae of the family Magnoliaceae. Magnolia extract can be obtained from the species within the Magnoliaceae family. Non-limiting examples of these species include Magnolia acuminata, Magnolia ashei, Magnolia biondii, Magnolia cylindrica, Magnolia cambellii, Magnolia denudata, Magnolia fraseri, Magnolia grandiflora, Magnolia hypoleuca, Magnolia kobus, Magnolia hliiflora, Magnolia loegneri, Magnolia macrophylla, Magnolia officinalis, Magnolia pyramidata, Magnolia sargentiana, Magnolia seiboldii, Magnolia soulangiana, Magnolia sprengeri, Magnolia stellata, Magnolia tripetala, Magnolia virginiana, Magnolia zenii, and Micheliafigo. A more complete listing of the species within the Magnoliaceae family can be found in Figlar & Nooteboom (2004), which is incorporated by reference.

0049[0033] Magnolia extract can reduce the blood flow near the skin surface though a variety of ways (e.g. , vasoconstriction, inhibition of angiogenesis, endothelial cell migration, or tube formation in or near the skin area that has been contacted with a composition containing Magnolia extract). Active ingredients that have been identified in Magnolia flower, bark, and seed cone extracts include magnolol, dihydroxydihdromagnolol, honokiol, and dihydrohonokiol. These are polyphenols containing compounds in which honokiol is an isomer of magnolol.

0050[0034] Magnolia extracts are commercial available from a variety of different sources. For instance Magnolia extracts can be purchased from Carrubba, Inc. (Milford Connecticut), Arcadia Herbs & Alternatives (Langhorne, Pennsylvania), and Herbal Extracts Plus (Croydon, Pennsylvania). Alternatively, a person of ordinary skill in the art would be able to isolate Magnolia extract from the Magnolia flower, bark, or seed cone by using any suitable isolation and purification methods known in the art {see e.g., Bai et al. (2003).

00512. Honokiol and Magnolol

0052[0035] The compositions of the present invention can include honokiol or magnolol or both. As noted above, these are biphenolic containing compounds in which honokiol is an isomer of magnolol. These compounds can be useful in reducing the blood flow near the skin surface through a variety of mechanisms {e.g., vasoconstriction, inhibition of angiogenesis, endothelial cell migration, or tube formation in or near the skin area that has been contacted with a composition containing honokiol or magnolol). The chemical structures of these compounds are illustrated below:

0053<img file="EP2144673A2_D0001.tif" />

0054Magnolol Honokiol

0055[0036] Honokiol and magnolol can be isolated or purified from Magnolia extracts

0056{e.g., flower, bark, and seed cone extracts) or other extracts that include such compounds by standard techniques. Alternatively, these compounds are commercially available {e.g., Wako Chemical Company (Tokyo, Japan)) or can be synthesized using convention chemical synthesis techniques known to those of ordinary skill in the art {see, e.g., Vollhardt and Schore, 1994).

00573. Chemical Compounds and Extracts

0058[0037] The compositions of the present invention can include humulus lupulus extract, gotu kola {centella asiatica extract), hesperidin methyl chalcone, dipeptide valyl- tryptophane, palmitoyl tetrapeptide-3, corylus avellana bud extract, cucumis sativa extract, morns alba extract, hibiscus sabdariffa flower extract, vitis vinifera extract, ascorbyl glucoside, citrus medica limonum extract, avena sativa kernel extract, hydrolyzed soy protein, aniseed myrtle extract, tasmania lanceolata leaf extract, artemisia abrotanum extract, citrus grandis fruit extract, or apigenin or any combination or mixture of such ingredients.

0059[0038] For instance, Humulus lupulus extract, or hops extract, can be used to reduce blood flow near the skin surface though the vasoconstriction, inhibition of angiogenesis, inhibition of endothelial cell migration or tube formation in or near the skin area that has been contacted with a composition containing hops extract. Hops extract is commercially available through a variety of sources {e.g. , Actives International (Allendale, New Jersey)) and can also be isolated or purified from Humulus lupulus plants by standard isolation and purification techniques. Non-limiting examples of varieties of Humulus lupulus from which hops extract can be obtained include Humulus lupulus var. lupulus, Humulus lupulus var. cordifolius, Humulus lupulus var. lupuloides (syn. H. americanus), Humulus lupulus var. neomexicanus, and Humulus lupulus var. pubescens. Humulus lupulus extract includes active ingredients such as humulene and lupulene.

0060[0039] Gotu kola (Centella asiatica) extract is a vine-like plant that is native to India and Southeast Asia. This ingredient can be used to strengthen capillary micro vessel barrier which can improve the overall function of blood vessels {e.g., efficient or improved blood circulation) in or near the skin area that has been contacted with a composition containing Gotu kola extract. Gotu kola extract is commercially available through a variety of sources {e.g., Naturex (South Hackensack, New Jersey)) and can also be isolated or purified from Gotu kola containing plants by standard isolation and purification techniques. Gotu kola extract contains active ingredients such as asiaticoside (a triterpene glycoside) (triterpenoid), brahmoside and brahminoside (both saponin glycosides), madecassoside (a glycoside with anti-inflammatory properties), madecassic acid, thiamine, riboflavin, pyridoxine, vitamin K, asparate, glutamate, serine, threonine, alanine, lysine, histidine, magnesium, calcium and sodium.

0061[0040] Hesperidin methyl chalcone, dipeptide valyl-tryptophane {i.e., dipeptide-2 which comprises valine and tryptophan), and palmitoyl tetrapeptide-3 (which is the reaction product of palmitic acid and a synthetic peptide containing glycine, glutamine, proline, and arginine) can also be included in compositions of the present invention. Hesperidin, a bioflavonoid which can be found in citrus peel such as the peel of sweet oranges (Citrus aurantium var. sinensis), can be converted into hesperidin methyl chalcone by extracting hesperidin from its source and placing the extract into an alkaline solution. This converts hesperidin into hesperidin chalcone which can subsequently be methylated by any known methylation process to produce hesperidin methyl chalcone. Hesperidin methyl chalcone can strengthen capillary micro vessel barrier in or near skin area that has been contacted with a composition including this ingredient. Dipetide valyl-tryptophane, which is commercially available under the trade name DIPEPTIDE VW™ through Sederma SAS (Cedex, France), can be used to mobilize fluid in skin tissue and drain the fluid from such tissue (which can reduce puffy eyes) when applied to the skin. Palmitoyl tetrapeptide-3, which is commercially available under the trade name N-PALMITOYL RIGIN™ through Sederma SAS (Cedex, France), can reduce local inflammation in skin tissue and restore skin firmness and elasticity when applied to skin. Further, a blend of these three ingredients is also commercially available under the trade name EYELISS™ through Dermaxime (Gauteng, South Africa).

0062[0041] Corylus (Hazel) extract can moisturize skin and can be used to mobilize fluid in skin tissue and drain the fluid from such tissue (which can reduce puffy eyes) when applied to the skin. The extract can be obtained from the bud, flower, leaves, nut, bark, etc. from the Hazel plant. Hazel extract is commercially available through a variety of sources {e.g., Mountain Rose Herbs, Eugene Oregon) and can also be isolated or purified from Hazel plants by standard isolation and purification techniques. Non-limiting examples of species of which Hazel extract can be obtained include Corylus americana, Corylus avellana, Corylus chinensis, Corylus colurna, Corylus cornuta, Corylus ferox, Corylus heterophylla, Corylus jacquemontii, Corylus maxima, Corylus sieboldiana, and Corylus tibetica.

0063[0042] Cucumis sativa (Cucumber) extract, morus alba extract, hibiscus sabdariffa flower extract, and vitis vinifera extract can be used in to brighten or even skin tone by inhibiting tyrosinase activity when applied to the skin. These ingredients are commercially available through a variety of sources and can also be isolated or purified from plants containing these extracts by standard isolation and purification techniques. In certain non limiting aspects, a blend of these ingredients can be used to obtain brighter or more even skin tone. Such a blend is commercially available under the trade name CLERILYS™ through GreenTech SA (Saint Beauzire, France). [0043] Ascorbyl glucoside can also be used to brighten or even skin tone by inhibiting tyrosinase activity when applied to the skin. Ascorbyl glucoside is a derivative of ascorbic acid (vitamin C) that includes an attached glucose sugar. In an ascorbyl glucoside molecule, typically, the glucose is typically attached at an OH group of ascorbic acid. The following is a non-limiting example of one form of ascorbyl glucoside, ascorbic acid-2 glucoside:

0064<img file="EP2144673A2_D0002.tif" />

0065Ascorbic Acid 2-Glucoside

0066Other non-limiting examples of ascorbyl glucoside include ascorbic acid 1 -glucoside (including 1-O-α-D-glucopyranosyl-L-ascorbic acid and 1-O-β-D-glucopyranosyl-L-ascorbic acid), ascorbic acid 2-glucoside (including 2-O-α-D-glucopyranosyl-L-ascorbic acid and 2-O- β-D-glucopyranosyl-L-ascorbic acid), ascorbic acid 3-glucoside (including 3-O-α-D- glucopyranosyl-L-ascorbic acid or 3-O-β-D-glucopyranosyl-L-ascorbic acid), ascorbic acid 5-glucoside (including 5-O-α-D-glucopyranosyl-L-ascorbic acid or 5-O-β-D-glucopyranosyl- L-ascorbic acid), and ascorbic acid 6-glucoside (including 6-O-α-D-glucopyranosyl-L- ascorbic acid or 6-O-β-D-glucopyranosyl-L-ascorbic acid). Ascorbyl glucoside is commercially available (e.g., Hayashibara Biochemical Laboratories, Inc.). The preparation of ascorbyl glucoside is also known in the art (see, e.g. U.S. Pat. Nos. 5,084,563; 5,252,722; 5,272,136; 5,388,420; 5,432,161 ; 5,843,907; and 5,508,391).

0067[0044] The compositions of the present invention can include an extract formulation comprising cucumis sativa (Cucumber) extract and citrus medica limonum (Lemon). Such a formulation can be used to brighten or even skin tone by inhibiting tyrosinase activity when applied to the skin. Such a formulation is commercially available under the trade name UNINONTAN U34™ through Chesham Chemicals, Ltd. (United Kingdom). Ingredients with the UNINONTAN U34™ formulation include cucumber extract (cucumis sativus) (15.0%), lemon extract (citrus medica limonum) (16.0%), sodium citrate (20.0%), propylene glycol (23.5%), and water (25.5%).

0068[0045] Non-limiting examples of anti-irritants and anti-oxidants that can be included in the compositions of the present invention include Avena sativa (Oat) extract, hydrolyzed soy protein, aniseed myrtle extract, tasmania lanceolata leaf extract, and Hibiscus sabdariffa (roselle) flower extract, or any combination or mixture of such ingredients. These ingredients are commercially available through a variety of sources and can also be isolated or purified from plants containing these extracts by standard isolation and purification techniques. For instance, a composition comprising Oat extract is commercially available under the trade name DRAGO CALM™ through Symrise (Holzminden, Germany). Hydrolyzed soy protein is commercially available under the trade name AQUA PRO SP™ through MGP Ingredients, Inc. (Atchison, Kansas). A blend of Anetholea anisata (aniseed myrtle) extract, Tasmania lanceolota (tasmanian mountain pepperberry) leaf extract, and Hibiscus sabdariffa (roselle) flower extract is commercially available under the trade name MOUNTAIN HARVEST™ through Southern Cross Botanicals (Knockrow, Australia).

0069[0046] Artemisia abrotanum (Southernwood) extract can stimulate adipogenesis and aid in the protection of the fat pad under the eye. This can be beneficial in combating fine lines and wrinkles and thinning/slackening skin. Southernwood extract is commercially available under the trade name PULPACTYL™ through Silab (Cedex, France) and can also be isolated or purified from plants containing these extracts by standard isolation and purification techniques.

0070[0047] Citrus grandis (Grapefruit) peel extract has anti-hyaluronidase, anti- angiogenesis, anti-and inflammatory properties when applied to skin. This ingredient can be used as a soothing agent for acute or chronic inflammation and can help repair skin damaged from excessive UV exposure. An active ingredient in Grapefruit extract is Apigenin. Grapefruit extract is commercially available under the trade name VIAPURE CITRUS™ through Actives International (Allendale, New Jersey) and can also be isolated or purified from plants containing these extracts by standard isolation and purification techniques.

00714. Cosmetic Ingredients

0072[0048] Compositions of the present invention can include other ingredients. Non- limiting examples of additional ingredients that can be added to cosmetic formulations can be found in the International Cosmetic Ingredient Dictionary, 10th Ed., 2004, which is incorporated by reference. Such ingredients include surfactants, preservatives, absorbents, adsorbents, chelating agents, lubricants, solvents, moisturizers (including, e.g., emollients, humectants, film formers, occlusive agents, and agents that affect the natural moisturization mechanisms of the skin), water repellents, anti-oxidants, UV absorbers, anti-irritants, vitamins, trace metals, anti-microbial agents, dyes and color ingredients, and/or structuring agents (see, e.g., McCutcheon's Functional Materials North American Edition 2001 and McCutcheon's Emulsifiers &amp; Detergents North American Edition 2001; U.S. Patent 6,290,938).

0073a. Surfactants

0074[0049] The compositions of the present invention can also comprise one or more surfactants. Surfactants can reduce the in interfacial tension between phases and improve the formulation and stability of a formulation. The surfactants can be nonionic, cationic, anionic, cryptoanionic, and zwitterionic emulsifiers (See McCutcheon's Emulsifiers &amp; Detergents (2001); U.S. Pat. Nos. 5,011,681; 4,421,769; 3,755,560, 6,117,915). Non-limiting examples include esters of glycerin, esters of propylene glycol, fatty acid esters of polyethylene glycol, fatty acid esters of polypropylene glycol, esters of sorbitol, esters of sorbitan anhydrides, carboxylic acid copolymers, esters and ethers of glucose, ethoxylated ethers, ethoxylated alcohols, alkyl phosphates, polyoxyethylene fatty ether phosphates, fatty acid amides, acyl lactylates, soaps, TEA stearate, DEA oleth-3 phosphate, polyethylene glycol 20 sorbitan monolaurate (polysorbate 20), polyethylene glycol 5 soya sterol, steareth-2, steareth-20, steareth-21, ceteareth-20, PPG-2 methyl glucose ether distearate, ceteth-10, cetyl phosphate, potassium cetyl phosphate, diethanolamine cetyl phosphate, polysorbate 20, polysorbate 60, polysorbate 80, glyceryl stearate, PEG-100 stearate, tyloxapol, and mixtures thereof.

0075b. Preservatives

0076[0050] Non-limiting examples of preservatives that can be used in the context of the present invention include quaternary ammonium preservatives such as polyquaternium-1 and benzalkonium halides (e.g., benzalkonium chloride ("BAC") and benzalkonium bromide), parabens (e.g., methylparabens and propylparabens), phenoxyethanol, benzyl alcohol, chlorobutanol, phenol, sorbic acid, thimerosal or combinations thereof. c. Moisturizers

0077[0051] Non-limiting examples of moisturizers include amino acids, chondroitin sulfate, diglycerin, erythritol, fructose, glucose, glycerin, glycerol polymers, glycol, 1,2,6- hexanetriol, honey, hyaluronic acid, hydrogenated honey, hydrogenated starch hydrolysate, inositol, lactitol, maltitol, maltose, mannitol, natural moisturizing factor, PEG- 15 butanediol, polyglyceryl sorbitol, salts of pyrollidone carboxylic acid, potassium PCA, propylene glycol, sodium glucuronate, sodium PCA, sorbitol, sucrose, trehalose, urea, and xylitol.

0078[0052] Other examples include acetylated lanolin, acetylated lanolin alcohol, acrylates/C 10-30 alkyl acrylate crosspolymer, acrylates copolymer, alanine, algae extract, aloe barbadensis, dλoe-barbadensis extract, aloe barbadensis gel, althea officinalis extract, aluminum starch octenylsuccinate, aluminum stearate, apricot (prunus armeniaca) kernel oil, arginine, arginine aspartate, arnica montana extract, ascorbic acid, ascorbyl palmitate, aspartic acid, avocado (persea gratissima) oil, barium sulfate, barrier sphingolipids, butyl alcohol, beeswax, behenyl alcohol, beta-sitosterol, BHT, birch {betula alba) bark extract, borage (borago officinalis) extract, 2-bromo-2-nitropropane-l,3-diol, butcherbroom {ruscus aculeatus) extract, butylene glycol, calendula officinalis extract, calendula officinalis oil, candelilla {euphorbia ceriferd) wax, canola oil, caprylic/capric triglyceride, cardamon (elettaria cardamomum) oil, carnauba {copernicia ceriferd) wax, carrageenan {chondrus crispus), carrot (daucus carota sativd) oil, castor (ricinus communis) oil, ceramides, ceresin, ceteareth-5, ceteareth-12, ceteareth-20, cetearyl octanoate, ceteth-20, ceteth-24, cetyl acetate, cetyl octanoate, cetyl palmitate, chamomile (anthemis nobilis) oil, cholesterol, cholesterol esters, cholesteryl hydroxystearate, citric acid, clary {salvia sclarea) oil, cocoa {theobroma cacao) butter, coco-caprylate/caprate, coconut {cocos nucifera) oil, collagen, collagen amino acids, corn (zea mays) oil, fatty acids, decyl oleate, dextrin, diazolidinyl urea, dimethicone copolyol, dimethiconol, dioctyl adipate, dioctyl succinate, dipentaerythrityl hexacaprylate/hexacaprate, DMDM hydantoin, DNA, erythritol, ethoxydiglycol, ethyl linoleate, eucalyptus globulus oil, evening primrose {Oenothera biennis) oil, fatty acids, tructose, gelatin, geranium maculatum oil, glucosamine, glucose glutamate, glutamic acid, glycereth-26, glycerin, glycerol, glyceryl distearate, glyceryl hydroxystearate, glyceryl lauratc, glyceryl linoleate, glyceryl myristate, glyceryl oleate, glyceryl stearate, glyceryl stearate SE, glycine, glycol stearate, glycol stearate SE, glycosaminoglycans, grape {vitis vinifera) seed oil, hazel {corylus americana) nut oil, hazel {corylus avelland) nut oil, hexylene glycol, honey, hyaluronic acid, hybrid safflower {carthamus tinctorius) oil, hydrogenated castor oil, hydrogenated coco-glycerides, hydrogenated coconut oil, hydrogenated lanolin, hydrogenated lecithin, hydrogenated palm glyceride, hydrogenated palm kernel oil, hydrogenated soybean oil, hydrogenated tallow glyceride, hydrogenated vegetable oil, hydrolyzed collagen, hydrolyzed elastin, hydrolyzed glycosaminoglycans, hydrolyzed keratin, hydrolyzed soy protein, hydroxylated lanolin, hydroxyproline, imidazolidinyl urea, iodopropynyl butylcarbamate, isocetyl stearate, isocetyl stearoyl stearate, isodecyl oleate, isopropyl isostearate, isopropyl lanolate, isopropyl myristate, isopropyl palmitate, isopropyl stearate, isostearamide DEA, isostearic acid, isostearyl lactate, isostearyl neopentanoate, jasmine (jasminum officinale) oil, jojoba {buxus chinensis) oil, kelp, kukui {aleurites moluccana) nut oil, lactamide MEA, laneth-16, laneth-10 acetate, lanolin, lanolin acid, lanolin alcohol, lanolin oil, lanolin wax, lavender (Lavandula angustifolia) oil, lecithin, lemon {citrus medica limonum) oil, linoleic acid, linolenic acid, macadamia ternifolia nut oil, magnesium stearate, magnesium sulfate, maltitol, matricaria (chamomilla recutita) oil, methyl glucose sesquistearate, methylsilanol PCA, microcrystalline wax, mineral oil, mink oil, mortierella oil, myristyl lactate, myristyl myristate, myristyl propionate, neopentyl glycol dicaprylate/dicaprate, octyldodecanol, octyldodecyl myristate, octyldodecyl stearoyl stearate, octyl hydroxystearate, octyl palmitate, octyl salicylate, octyl stearate, oleic acid, olive {plea europaea) oil, orange {citrus aurantium dulcis) oil, palm {elaeis guineensis) oil, palmitic acid, pantethine, panthenol, panthenyl ethyl ether, paraffin, PCA, peach {prunus persica) kernel oil, peanut {arachis hypogaea) oil, PEG-8 C12-18 ester, PEG-15 cocamine, PEG-150 distearate, PEG-60 glyceryl isostearate, PEG-5 glyceryl stearate, PEG-30 glyceryl stearate, PEG-7 hydrogenated castor oil, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PEG-20 methyl glucose sesquistearate, PEG40 sorbitan peroleate, PEG-5 soy sterol, PEG-IO soy sterol, PEG-2 stearate, PEG-8 stearate, PEG-20 stearate, PEG-32 stearate, PEG40 stearate, PEG-50 stearate, PEG-100 stearate, PEG-150 stearate, pentadecalactone, peppermint {mentha piperita) oil, petrolatum, phospholipids, polyamino sugar condensate, polyglyceryl-3 diisostearate, polyquaternium-24, polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, polysorbate 85, potassium myristate, potassium palmitate, potassium sorbate, potassium stearate, propylene glycol, propylene glycol dicaprylate/dicaprate, propylene glycol dioctanoate, propylene glycol dipelargonate, propylene glycol laurate, propylene glycol stearate, propylene glycol stearate SE, PVP, pyridoxine dipalmitate, quaternium-15, quaternium-18 hectorite, quaternium-22, retinol, retinyl palmitate, rice {oryza sativa) bran oil, RNA, rosemary Rosmarinus officinalis) oil, rose oil, sage {salvia officinalis) oil, salicylic acid, sandalwood {santalum album) oil, serine, serum protein, sesame {sesamum indicum) oil, shea butter (butyrospermum parkii), silk powder, sodium chondroitin sulfate, sodium hyaluronate, sodium lactate, sodium palmitate, sodium PCA, sodium polyglutamate, sodium stearate, soluble collagen, sorbic acid, sorbitan laurate, sorbitan oleate, sorbitan palmitate, sorbitan sesquioleate, sorbitan stearate, sorbitol, soybean (glycine soja) oil, sphingolipids, squalane, squalene, stearamide MEA-stearate, stearic acid, stearoxy dimethicone, stearoxytrimethylsilane, stearyl alcohol, stearyl glycyrrhetinate, stearyl heptanoate, stearyl stearate, sunflower {helianthus annum) seed oil, sweet almond (prunus amygdalus dulcis) oil, synthetic beeswax, tocopherol, tocopheryl acetate, tocopheryl linoleate, tribehenin, tridecyl neopentanoate, tridecyl stearate, triethanolamine, tristearin, urea, vegetable oil, water, waxes, wheat (triticum vulgare) germ oil, and ylang ylang (cananga odoratd) oil.

0079d. Emollients

0080[0053] Examples of emollients include, but are not limited to, vegetable oils, mineral oils, silicone oils, synthetic and natural waxes, medium chain triglycerides, petrolatum, lanolin, aluminum magnesium hydroxide stearate (which can also function as a water repellent), and fatty acid esters. Non-limiting examples of vegetable oils include safflower oil, corn oil, sunflower seed oil, and olive oil.

0081e. Antioxidants

0082[0054] Examples of antioxidants include, but are not limited to, acetyl cysteine, ascorbic acid, ascorbic acid polypeptide, ascorbyl dipalmitate, ascorbyl methylsilanol pectinate, ascorbyl palmitate, ascorbyl stearate, BHA, BHT, t-butyl hydroquinone, cysteine, cysteine HCl, diamylhydroquinone, di-t-butylhydroquinone, dicetyl thiodipropionate, dioleyl tocopheryl methylsilanol, disodium ascorbyl sulfate, distearyl thiodipropionate, ditridecyl thiodipropionate, dodecyl gallate, erythorbic acid, esters of ascorbic acid, ethyl ferulate, ferulic acid, gallic acid esters, hydroquinone, isooctyl thioglycolate, kojic acid, magnesium ascorbate, magnesium ascorbyl phosphate, methylsilanol ascorbate, natural botanical antioxidants such as green tea or grape seed extracts, nordihydroguaiaretic acid, octyl gallate, phenylthioglycolic acid, potassium ascorbyl tocopheryl phosphate, potassium sulfite, propyl gallate, quinones, rosmarinic acid, sodium ascorbate, sodium bisulfite, sodium erythorbate, sodium metabisulfite, sodium sulfite, superoxide dismutase, sodium thioglycolate, sorbityl furfural, thiodiglycol, thiodiglycolamide, thiodiglycolic acid, thioglycolic acid, thiolactic acid, thiosalicylic acid, tocophereth-5, tocophereth-10, tocophereth-12, tocophereth-18, tocophereth-50, tocopherol, tocophersolan, tocopheryl acetate, tocopheryl linoleate, tocopheryl nicotinate, tocopheryl succinate, and tris(nonylphenyl)phosphite.

0083f. Compounds Having Ultraviolet Light Absorbing Properties

0084[0055] Non-limiting examples of compounds that have ultraviolet light absorbing properties that can be used with the compounds of the present invention include benzophenone, benzophenone-1, benzophenone-2, benzophenone-3, benzophenone-4 benzophenone-5, benzophenone-6, benzophenone-7, benzophenone-8, benzophenone-9, benzophenone- 10, benzophenone-11, benzophenone- 12, benzyl salicylate, butyl PABA, cinnamate esters, cinoxate, DEA-methoxycinnamate, diisopropyl methyl cinnamate, ethyl dihydroxypropyl PABA, ethyl diisopropylcinnamate, ethyl methoxycinnamate, ethyl PABA, ethyl urocanate, glyceryl octanoate dimethoxycinnamate, glyceryl PABA, glycol salicylate, homosalate, isoamyl p-methoxycinnamate, PABA, PABA esters, Parsol 1789, isopropylbenzyl salicylate, and octyl methoxycinnamate.

0085g. Structuring Agents

0086[0056] In other non-limiting aspects, the compositions of the present invention can include a structuring agent. Structuring agent, in certain aspects, assist in providing rheological characteristics to the composition to contribute to the composition's stability. In other aspects, structuring agents can also function as an emulsifϊer or surfactant. Non- limiting examples of structuring agents include stearic acid, palmitic acid, stearyl alcohol, cetyl alcohol, behenyl alcohol, stearic acid, palmitic acid, the polyethylene glycol ether of stearyl alcohol having an average of about 1 to about 21 ethylene oxide units, the polyethylene glycol ether of cetyl alcohol having an average of about 1 to about 5 ethylene oxide units, and mixtures thereof. Other non-limiting examples can be found in International Cosmetic Ingredient Dictionary, 1 Oth edition, 2004, which is incorporated by reference.

0087h. Silicone Containing Compounds

0088[0057] In non-limiting aspects, silicone containing compounds include any member of a family of polymeric products whose molecular backbone is made up of alternating silicon and oxygen atoms with side groups attached to the silicon atoms. By varying the -Si- O- chain lengths, side groups, and cross linking, silicones can be synthesized into a wide variety of materials. They can vary in consistency from liquid to gel to solids. [0058] The silicone containing compounds that can be used in the context of the present invention include those described in this specification or those known to a person of ordinary skill in the art. Non-limiting examples include silicone oils (e.g., volatile and nonvolatile oils), gels, and solids. In certain aspects, the silicon containing compounds includes a silicone oils such as a polyorganosiloxane. Non-limiting examples of polyorganosiloxanes include dimethicone, cyclomethicone, polysilicone-11, phenyl trimethicone, trimethylsilylamodimethicone, stearoxytrimethylsilane, or mixtures of these and other organosiloxane materials in any given ratio in order to achieve the desired consistency and application characteristics depending upon the intended application (e.g., to a particular area such as the skin, hair, or eyes). A "volatile silicone oil" includes a silicone oil have a low heat of vaporization, i.e. normally less than about 50 cal per gram of silicone oil. Non- limiting examples of volatile silicone oils include: cyclomethicones such as Dow Corning 344 Fluid, Dow Corning 345 Fluid, Dow Corning 244 Fluid, and Dow Corning 245 Fluid, Volatile Silicon 7207 (Union Carbide Corp., Danbury, Conn.); low viscosity dimethicones, i.e., dimethicones having a viscosity of about 50 cst or less (e.g., dimethicones such as Dow Corning 200-0.5 cst Fluid). The Dow Corning Fluids are available from Dow Corning Corporation, Midland, Michigan. Cyclomethicone and dimethicone are described in International Cosmetic Ingredient Dictionary, 10th edition, 2004, which is incorporated by reference as cyclic dimethyl polysiloxane compounds and a mixture of fully methylated linear siloxane polymers end-blocked with trimethylsiloxy units, respectively. Other non- limiting volatile silicone oils that can be used in the context of the present invention include those available from General Electric Co., Silicone Products Div., Waterford, N.Y. and SWS Silicones Div. of Stauffer Chemical Co., Adrian, Michigan.

0089i. Essential Oils

0090[0059] Essential oils include oils derived from herbs, flowers, trees, and other plants.

0091Such oils are typically present as tiny droplets between the plant's cells, and can be extracted by several method known to those of skill in the art (e.g., steam distilled, enfleurage (i.e., extraction by using fat), maceration, solvent extraction, or mechanical pressing). When these types of oils are exposed to air they tend to evaporate (i.e., a volatile oil). As a result, many essential oils are colorless, but with age they can oxidize and become darker. Essential oils are insoluble in water and are soluble in alcohol, ether, fixed oils (vegetal), and other organic solvents. Typical physical characteristics found in essential oils include boiling points that vary from about 160° to 240° C and densities ranging from about 0.759 to about 1.096. [0060] Essential oils typically are named by the plant from which the oil is found.

0092For example, rose oil or peppermint oil are derived from rose or peppermint plants, respectively. Non-limiting examples of essential oils that can be used in the context of the present invention include sesame oil, macadamia nut oil, tea tree oil, evening primrose oil, Spanish sage oil, Spanish rosemary oil, coriander oil, thyme oil, pimento berries oil, rose oil, anise oil, balsam oil, bergamot oil, rosewood oil, cedar oil, chamomile oil, sage oil, clary sage oil, clove oil, cypress oil, eucalyptus oil, fennel oil, sea fennel oil, frankincense oil, geranium oil, ginger oil, grapefruit oil, jasmine oil, juniper oil, lavender oil, lemon oil, lemongrass oil, lime oil, mandarin oil, marjoram oil, myrrh oil, neroli oil, orange oil, patchouli oil, pepper oil, black pepper oil, petitgrain oil, pine oil, rose otto oil, rosemary oil, sandalwood oil, spearmint oil, spikenard oil, vetiver oil, wintergreen oil, or ylang ylang. Other essential oils known to those of skill in the art are also contemplated as being useful within the context of the present invention.

0093j. Thickening Agents

0094[0061] Thickening agents, including thickener or gelling agents, include substances which that can increase the viscosity of a composition. Certain thickeners can increase the viscosity of a composition without substantially modifying the efficacy of the active ingredient within the composition. Thickeners can also increase the stability of the compositions of the present invention.

0095[0062] Non-limiting examples of thickening agents that can be used in the context of the present invention include hydrogenated polyisobutene or trihydroxystearin or combination of both. Other examples include carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides, and gums. Examples of carboxylic ac id polymers include crosslinked compounds containing one or more monomers derived from acrylic acid, substituted acrylic acids, and salts and esters of these acrylic acids and the substituted acrylic acids, wherein the crosslinking agent contains two or more carbon- carbon double bonds and is derived from a polyhydric alcohol (see U.S. Pat. Nos. 5,087,445; 4,509,949; 2,798,053; CTFA International Cosmetic Ingredient Dictionary, Tenth Edition, 2004). Examples of commercially available carboxylic acid polymers include carbomers, which are homopolymers of acrylic acid crosslinked with allyl ethers of sucrose or pentaerytritol (e.g., Carbopol™ 900 series from B. F. Goodrich). [0063] Non-limiting examples of crosslinked polyacrylate polymers include cationic and nonionic polymers. Examples are described in U.S. Pat. Nos. 5,100,660; 4,849,484; 4,835,206; 4,628,078; 4,599,379.

0096[0064] Non-limiting examples of polyacrylamide polymers (including nonionic polyacrylamide polymers including substituted branched or unbranched polymers) include polyacrylamide, isoparaffϊn and laureth-7, multi-block copolymers of acrylamides and substituted acrylamides with acrylic acids and substituted acrylic acids.

0097[0065] Non-limiting examples of polysaccharides include cellulose, carboxymethyl hydroxyethylcellulose, cellulose acetate propionate carboxylate, hydroxyethylcellulose, hydroxyethyl ethylcellulose, hydroxypropylcellulose, hydroxypropyl methylcellulose, methyl hydroxyethylcellulose, microcrystalline cellulose, sodium cellulose sulfate, and mixtures thereof. Another example is an alkyl substituted cellulose where the hydroxy groups of the cellulose polymer is hydroxyalkylated (e.g., hydroxy ethylated or hydroxypropylated) to form a hydroxyalkylated cellulose which is then further modified with a C10-C30 straight chain or branched chain alkyl group through an ether linkage. Typically these polymers are ethers of C10-C30 straight or branched chain alcohols with hydroxyalkyl celluloses. Other useful polysaccharides include scleroglucans comprising a linear chain of (1-3) linked glucose units with a (1-6) linked glucose every three unit.

0098[0066] Non-limiting examples of gums that can be used with the present invention include acacia, agar, algin, alginic acid, ammonium alginate, amylopectin, calcium alginate, calcium carrageenan, carnitine, carrageenan, dextrin, gelatin, gellan gum, guar gum, guar hydroxypropyltrimonium chloride, hectorite, hyaluroinic acid, hydrated silica, hydroxypropyl chitosan, hydroxypropyl guar, karaya gum, kelp, locust bean gum, natto gum, potassium alginate, potassium carrageenan, propylene glycol alginate, sclerotium gum, sodium carboyxmethyl dextran, sodium carrageenan, tragacanth gum, xanthan gum, and mixtures thereof.

0099k. Additional Ingredients

0100[0067] Non-limiting examples of additional compounds and agents that can be used with the compositions of the present invention include, vitamins (e.g., D, E, A, K, and C), trace metals (e.g. , zinc, calcium and selenium), anti-irritants (e.g. , steroids and non-steroidal antiinflammatories), botanical extracts (e.g. aloe vera, chamomile, cucumber extract, ginkgo biloba, ginseng, and rosemary), dyes and color ingredients (e.g., D&amp;C blue no. 4, D&amp;C green no. 5, D&amp;C orange no. 4, D&amp;C red no. 17, D&amp;C red no. 33, D&amp;C violet no. 2, D&amp;C yellow no. 10, D&amp;C yellow no. 11 and DEA-cetyl phosphate), emollients (i.e., organic esters, fatty acids, lanolin and its derivatives, plant and animal oils and fats, and di- and triglycerides), antimicrobial agents (e.g., triclosan and ethanol), and fragrances (natural and artificial).

0101B. Source of Ingredients

0102[0068] The ingredients of the compositions of the present invention can be obtained by any means known to a person of ordinary skill in the art. In a non-limiting embodiment, for example, the ingredients can be isolated by obtaining the source of such ingredient. In many instances, the ingredients are also commercially available as explained above. For example, Magnolia extracts can be obtained through any number of companies including Carrubba, Inc. (Milford Connecticut), Arcadia Herbs &amp; Alternatives (Langhorne, Pennsylvania), and Herbal Extracts Plus (Croydon, Pennsylvania). Additionally, the compounds, agents, and active ingredients can be purified by any number of techniques known to a person of ordinary skill in the art. Non-limiting examples of purification techniques include Polyacrylamide Gel Electrophoresis, High Performance Liquid Chromatography (HPLC), Gel chromatography or Molecular Sieve Chromatography, and Affinity Chromatography. In other aspects, the compounds, agents, and active ingredients can be obtained by chemical synthesis or by recombinant means by using conventional techniques. See, for example, Stewart and Young, (1984); Tarn et al, (1983); Merrifield, (1986); and Barany and Merrifield (1979), Houghten (1985).

0103C. Modifications and Derivatives

0104[0069] Modifications or derivatives of the ingredients disclosed throughout this specification are also contemplated as being useful with the methods and compositions of the present invention. Derivatives may be prepared and the properties of such derivatives may be assayed for their desired properties by any method known to those of skill in the art.

0105[0070] In certain aspects, "derivative" refers to a chemically modified compound that still retains the desired effects of the compound prior to the chemical modification. Such derivatives may have the addition, removal, or substitution of one or more chemical moieties on the parent molecule. Non limiting examples of the types modifications that can be made to the compounds and structures disclosed throughout this document include the addition or removal of lower alkanes such as methyl, ethyl, propyl, or substituted lower alkanes such as hydroxymethyl or aminomethyl groups; carboxyl groups and carbonyl groups; hydroxyls; nitro, amino, amide, and azo groups; sulfate, sulfonate, sulfono, sulfhydryl, sulfonyl, sulfoxido, phosphate, phosphono, phosphoryl groups, and halide substituents. Additional modifications can include an addition or a deletion of one or more atoms of the atomic framework, for example, substitution of an ethyl by a propyl; substitution of a phenyl by a larger or smaller aromatic group. Alternatively, in a cyclic or bicyclic structure, hetero atoms such as N, S, or O can be substituted into the structure instead of a carbon atom.

0106D. Equivalents

0107[0071] Known and unknown equivalents to the ingredients discussed throughout this specification and claims can be used with the compositions and methods of the present invention. The equivalents can be used as substitutes for any given ingredient in a composition of the present invention. The equivalents can also be used to add to the methods and compositions of the present invention. A person of ordinary skill in the art would be able to recognize and identify acceptable known and unknown equivalents to the ingredients without undue experimentation.

0108E. Compositions of the Present Invention

0109[0072] A person of ordinary skill would recognize that the compositions of the present invention can include any number of combinations of the ingredients discussed throughout this specification. It is also contemplated that that the concentrations of the ingredients can vary. In non-limiting embodiments, for example, the compositions may include in their final form, for example, at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012%, 0.0013%, 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026%, 0.0027%, 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042%, 0.0043%, 0.0044%, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077%, 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%, 0.0095%, 0.0096%, 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000%, 0.2250%, 0.2500%, 0.2750%, 0.3000%, 0.3250%, 0.3500%, 0.3750%, 0.4000%, 0.4250%, 0.4500%, 0.4750%, 0.5000%, 0.5250%, 0.0550%, 0.5750%, 0.6000%, 0.6250%, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9%, 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% or more, or any range or integer derivable therein, of at least one of the ingredients mentioned throughout the specification and claims. In non-limiting aspects, the percentage can be calculated by weight or volume of the total composition. A person of ordinary skill in the art would understand that the concentrations can vary depending on the desired effect of the composition and/or on the product into which the composition is incorporated into.

0110F. Cosmetic Vehicles

0111[0073] The compositions of the present invention can be incorporated in a variety of different vehicles. Non-limiting examples of suitable vehicles include emulsions (e.g., oil/water emulsion, an oil/water/oil emulsion, a water/oil emulsion, a water/oil/water emulsion, a water/silicone emulsion, a water/silicone/water emulsion, a silicone/water emulsion, a silicone/water/silicone emulsion, a water/wax emulsion, or an oil/water/silicone emulsion), creams, lotions, solutions (both aqueous and hydro-alcoholic), anhydrous bases (e.g., lipsticks and powders), gels, ointments, serums, liquids, fluids, non-aerosol sprays, aerosol sprays, non-aerosol foams, aerosol foams or by other method or any combination of the forgoing as would be known to one of ordinary skill in the art (Remington's, 1990). Variations and other appropriate vehicles will be apparent to the skilled artisan and are appropriate for use in the present invention. In certain aspects, it is important that the concentrations and combinations of ingredients be selected in such a way that the combinations are chemically compatible and do not form complexes which precipitate from the finished product.

0112G. Cosmetic Products

0113[0074] The composition of the present invention can also be used in many cosmetic products including, but not limited to, concealers, foundations, sunscreen products, sunless skin tanning products, moisturizing creams, skin benefit creams and lotions, softeners, day lotions, gels, ointments, night creams, lipsticks, cleansers, toners, masks, hair products, finger nail products, and other known cosmetic products or applications.

0114H. Kits

0115[0075] Kits are also contemplated as being used in certain aspects of the present invention. For instance, compositions of the present invention can be included in a kit. A kit can include a container. Containers can include a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressurized container, a barrier container, a package, a compartment, a lipstick container, a compact container, cosmetic pans that can hold cosmetic compositions (e.g., foundations), or other types of containers such as injection or blow- molded plastic containers into which the dispersions or compositions or desired bottles, dispensers, or packages are retained. The kit and/or container can include indicia on its surface. The indicia, for example, can be a word, a phrase, an abbreviation, a picture, or a symbol.

0116[0076] The containers can dispense a pre-determined amount of the composition. In other embodiments, the container can be squeezed (e.g., metal, laminate, or plastic tube) to dispense a desired amount of the composition. The composition can be dispensed as a spray, an aerosol, a liquid, a fluid, or a semi-solid. The containers can have spray, pump, or squeeze mechanisms. A kit can also include instructions for employing the kit components as well the use of any other dispersions or compositions included in the container. Instructions can include an explanation of how to apply, use, and maintain the products, dispersions, or compositions. EXAMPLES

0117[0077] The following examples are included to demonstrate certain non-limiting aspects of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function well in the practice of the invention. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

EXAMPLE 1

0119[0078] A non-limiting example of a composition of the present invention is described in Table 1. This composition is a cream that can be topically applied to skin.

0120Table 1*

0121<img file="EP2144673A2_D0003.tif" /><img file="EP2144673A2_D0004.tif" />

0122*The Natural extract blend contains 6.3% Citrus Tangerina (tangerine) extract, 63.7% Citrus aurantium dulcis (orange) peel extract, and 30% phenoxyethanol (preservative).

0123[0079] The following non-limiting procedure was used to prepare the composition in

0124Table 1. All Phase A ingredients were added to water in a main vessel and dispersed by propeller and sweep mixing. The mixture was heated to 55-60 ⁰C and while mixing. In a separate vessel, the Phase B ingredients were heated to 55-60 ⁰C or until the solids melted. The Phase B mixture was added to the main vessel and mixed for approximately fifteen (15) minutes. The heat source was removed. The Phase C ingredients were added to the main vessel in the order listed in Table 1 while mixing. Before the Phase D ingredients were added, the mixture resembled a smooth, thick, white cream. The Phase D ingredients were added to the main vessel at approximately 50 ⁰C while mixing (note that the Phase D ingredients can be made into a slurry prior to adding to the main vessel, if needed). As the Phase D ingredients are added, the mixture will thin back down and turn a yellow shade. The Phase E ingredients are then added at 40-45 ⁰C. Mixing is continued as the mixture is cooled to 30-35 ⁰C.

EXAMPLE 2

0126[0080] Testing Parameters of the Table 1 Composition: The efficacy of the composition in Table 1 was tested on human skin. The composition was tested on one- hundred and twenty three (123) women ("panelists") having the following characteristics: (a) 1/2 of the women were between the ages of 32 to 45 and 1/2 were between 46 to 60 years of age; (b) 1/3 of the women had dry/dry to normal skin, 1/3 had normal skin and 1/3 had combination skin/oily skin; (c) 80% of the women had noticeably mild/moderate "under eye puffiness;" and (d) 50% of the women used a facial concealer product 3 or more times a week. A summary of the panelists' skin conditions is in Table 2. The composition was applied to the under eye skin area twice a day (once in the morning and evening) for a total of fourteen (14) days. The panelists filled out questionnaires that inquired into the tactile properties and efficacy of the composition.

0127Table 2 (Panelist Skin Conditions)

0128AGE SKIN TYPE® SKIN TONE

0129Total 35 - 45 46 - 60 Dry Normal Oily Light Medium Dark

0130Base Total Respondents (123) ( 57) ( 66) ( 41) ( 38) ( 44) ( 49)® ( 50) ( 24)^β

0131J-O. _%_ _%. J-S- _%_ _% J-L J&amp;. %.

0132Crow's Feet 55 37 71 59 61 48 53 60 50

0133Dark Circles 100 100 100 100 100 100 100 100 100 Mild 69 83 58 66 66 75 61 74 75 Moderate 31 17 42 34 34 25 39 26 25

0134Undereve Puffiness 85 77 92 85 8Z 84 84 86 88 Mild 64 68 61 63 66 64 55 68 75 Moderate 21 9 31 22 21 20 29 18 13

0135[0081] Results of the Table 1 Composition: The panelists' responses are summarized in the following Tables 3-16. The responses in Table 3 concern the tactile properties and efficacy of the composition immediately after application to the skin.

0136Table 3 (Immediately After Application)

0137<img file="EP2144673A2_D0005.tif" />

0138<img file="EP2144673A2_D0006.tif" />

0139* Excluding "does not apply to me/don't know"

0140[0082] The panelists' responses in Table 4 concern the efficacy of the composition after two weeks of use.

0141Table 4 (After Two Weeks of Use)

0142<img file="EP2144673A2_D0007.tif" /> [0083] The responses in Table 5 concern the tactile properties and efficacy of the composition immediately after application to the skin. The responses are organized by panelist age.

0143Table 5 (Age—Immediately After Application)

0144Immediately After Application - ^■■ % Agree " ^AGE

014532-45 46-60

0146.%. %-

0147Is easy to apply 97 99

0148Is lightweight 96 99

0149Leaves a soft afterfeel 93 99

0150My regular eye cream applies easily over the test product 93 99

0151My regular foundation applies easily over the test product 91 100

0152Does not feel oily/greasy during application 90 96

0153Provides a cooling sensation upon application 91 94

0154Does not leave an oily/greasy afterfeel 90 92

0155Leaves a smooth afterfeel 88 92

0156Is suitable for rny skin type 90 91

0157Has a silky-smooth texture 91 83

0158Absorbs quickly 84 85

0159Provides a cooling sensation that lasts a few minutes 73 89

0160Visibly reduces appearance of undereye puffiness immediately after application 55 57

0161Visibly minimizes appearance of undereye puffiness immediately after application 48 51

0162Visibly minimizes appearance of dark circles immediately after application 45 53

0163Visibly reduces appearance of dark circles immediately after application 38 49

0164[0084] The panelists' responses in Table 6 concern the efficacy of the composition after two weeks of use. The responses are organized by panelist age.

0165Table 6 (Age-After Two Weeks of Use)

0166Two Weeks After Use - -% Agree^"' AGE

016732-45 46-60

0168_%. -%.

0169Dark Circles

0170Visibly minimizes a ppearance of d ark circles 73 65 Improves app earance of da rk circles 67 68 Visibly reduces appearance of dark circles 65 66 Diminishes appe arance of dark circles 71 59 Softe ns a ppearance of d ark circles 80 68

0171Softe ns a ppearance of u ndereye puffiness 83 77 Visibly minimizes appearance of undereye puffiness 73 77 Improves appearance of un dereye puffiness 77 71 Visibly reduces ap pearance of un dereye puffiness 74 72 Diminishes appe arance of unde reye puffiness 71 69

0172Raqqy Rkin

0173Softe ns a ppearance of u ndereye sagginess 71 68 Improves appearance of un dereye sagginess 63 68 Visibly reduces ap pearance of un dereye sagginess 59 66 Visibly minimizes a ppearance of u ndereye sagginess 59 63 Diminishes appe arance of undereye sagginess 52 60

0174General Appearance Of Eye Area Freshens my e ye area 87 89 Rejuvenates eye area 84 72 Leaves eye area with a rested look 76 73 Revitalizes eye area 75 71 Leaves eye area looking younger 75 63 Brightens up eye area 71 65 Leaves eye area lookinq vibrant 68 64

0175[0085] The panelists' responses in Table 7 concern the tactile properties and efficacy of the composition immediately after application to the skin. The responses are organized by panelists who have mild/moderate under eye puffiness and panelists who do not have under eye puffiness.

0176Table 7 (Under Eye Puffiness—Immediately After Application)

0177Immediately After Application - ^■'% Agree ^" UNDEREYE PUFFINESS

0178H ave Mild/ Do Not Moderate Have Undereye Undereye Puffiness Puffiness

0179_%. -%.

0180Is easy to apply 97 100

0181Is lightweight 98 94

0182Leaves a soft afterfeel 96 94

0183My regula r eye cream applies easily over the test product 97 88

0184My regular foundation applies easily overthe test product 97 89

0185Does not feel oily/greasy during app lication 94 89

0186Provides a cooling sensation upon application 92 94

0187Does not leave an oily/greasy afterfeel 92 83

0188Leaves a smooth afterfeel 90 89

0189Is suitable for my skin type 90 89

0190Has a silky- smooth texture 85 100

0191Absorbs quickly 85 83

0192Provides a cooling sensation that lasts a few minutes 85 65

0193Visibly reduces appearance of undereye puffiness immediately after application 58 41

0194Visibly minimizes appearance of undereye puffiness immediately after application 52 33

0195Visibly minimizes appearance of dark circles immediately after application 49 50

0196Visibly reduces appearance of dark circles immediately after application 44 39

0197[0086] The panelists' responses in Table 8 concern the efficacy of the composition after two weeks of use. The responses are organized by panelists who have mild/moderate under eye puffiness and panelists who do not have under eye puffiness.

0198Table 8 (Undereye Puffiness—After Two Weeks of Use)

0199TWo Weeks After Use - ^li% Agree" UNDEREYE PUFFINESS

0200Have M iltf Do Not

0201M oderate Have

0202Undereye Undereye

0203Puffiness Puffiness

JL

0205Dark Circles

0206Visibly minimizes appearance of dark circles 66 83 Improves appearance of dark circles 65 83 Visibly reduc es appearance of dark c irc les 64 78 Diminishes appearance of dark circles 63 72 S oftens appearance of dark circ les 71 89

0207S oftens appearance of under eye puffiness 78 93 Visibly minimizes appearance of undereye puffiness 72 93 Improves appearance of undereye puffiness 72 87 V isibly reduc es appearance of undereye puffiness 69 93 Diminishes appearanc e of undereye puffiness 68 80

0208Sapgv S kin

0209S oftens appearance of undereye sagginess 68 83 Improves appearance of undereye sagginess 63 85 Visibly reduc es appearance of undereye sagginess 60 85 Visibly minimizes appearance of undereye sagginess 57 85 Diminishes appearance of undereye sagginess 54 69

0210General Appearance Of Ev e Area F reshens my eye area 89 88 Rejuvenates eye area 75 94 Leaves eye area with a rested look 74 82 Revitalizes eye area 70 89 Leaves eye area looking younger 66 83 B rightens up eye area 66 78 Leaves eye area looking vibrant 64 78

0211[0087] The panelists' responses in Table 9 concern the tactile properties and efficacy of the composition immediately after application to the skin. The responses are organized by panelists who have mild dark circles under the eye and panelists who do not have mild dark circles under the eye.

0212Table 9 (Dark Circles—Immediately After Application)

0213Immediately After Application - "% Agree"

DARK CIRCLES

0215M ild M oderate

0216% %

0217I s easy to apply 98 97

0218I s lightweight 98 97

0219Leaves a soft afterf eel 97 95

0220My regular eye cream applies easily over the test product 95 97

0221My regular foundation applies easily over the test product 94 100

0222Does not feel oily/greasy during application 93 92

0223Provides a cooling sensation upon application 94 90

0224Does not leave an oily/greasy afterfeel 89 95

0225Leaves a smooth afterfeel 93 84

0226I s suitable for my skin type 91 89

0227Has a silky-smooth texture 91 78

0228Absorbs quickly 86 82

0229Provides a cooling sensation that lasts a few minutes 82 82

0230Visibly reduces appearance of undereye puffiness immediately after application BO 46

0231Visibly minimizes appearance of undereye puffiness immediately after application 54 39

0232Visibly minimizes appearance of dark circles immediately after application 52 43

0233Visibly reduces appearance of dark circles immediately after application 44 42

0234[0088] The panelists' responses in Table 10 concern the efficacy of the composition after two weeks of use. The responses are organized by panelists who have mild dark circles under the eye and panelists who do not have mild dark circles under the eye.

0235Table 10 (Dark Circles-After Two Weeks of Use)

0236Two Weeks After Use - "% Agree" _{mRK C|RCL£S}

0237Mild Moderate

0238Dark Circles

0239Visibly minimizes appearance of dark circles 70 66 Improves appearance of dark circles 69 66 Visibly reduces appearance of dark circles 67 63 Diminishes appearance of dark circles 68 58 Softens appearance of dark circles 76 68

0240Softens appearance of undereye puffiness 80 81 Visibly minimizes appearance of undereye puffiness 74 77 Improves appearance of undereye puffiness 75 71 Visibly reduces appearance of undereye puffiness 73 71 Diminishes appearance of undereye puffiness 74 59

0241Saggy Skin

0242Softens appearance of undereye sagginess 74 60 Improves appearance of undereye sagginess 69 60 Visibly reduces appearance of undereye sagginess 64 60 Visibly rninimixes appearance of undereye sagginess 63 57 Diminishes appearance of undereye sagginess 58 53

0243GenRral Appearance Of Eyp Area Freshens my eye area 88 90 Rejuvenates eye area 77 78 Leaves eye area witti a rested look 72 81 Revitalizes eye area 74 71 Leaves eye area looking younger 68 71 Brightens up eye area 66 71 Leaves eye area looking vibrant 66 65

0244[0089] The panelists' responses in Table 11 concern the tactile properties and efficacy of the composition immediately after application to the skin. The responses are organized by panelists who use a concealer product and panelists who do not use a concealer product.

0245Table 11 (Concealer Use—Immediately After Application)

0246immediately After Application - "% Agree"

USE OF CONCEALER

0248Use Do Not Use

0249Concealer Concealer

0250.%. %. l s easy to apply 97 98

0251I s lightweight 98 100

0252Leaves a soft afterfeel 94 98

0253My regular eye cream applies easily over the test product 99 92

0254My regular foundation applies easily over the test product 99 92

0255Does not feel oily/greasy during application 94 91

0256Provides a cooling sensation upon application 89 96

0257Does not leave an oily/greasy afterfeel 93 89

0258Leaves a smooth afterfeel 85 96

0259I s suitable for rny skin type 93 87

0260Has a silky-smooth texture 88 85

0261Absorbs quickly 84 85

0262Provides a cooling sensation that lasts a few minutes 81 83

0263Visibly reduces appearance of undεreye puffiness immediately after application 58 53

0264Visibly minimizes appearance of undereye puffiness immediately after application 52 47

0265Visibly minimizes appearance of dark circles immediately after application 55 42

0266Visibly reduces appearance of dark circles immediately after application 47 39

0267[0090] The panelists' responses in Table 12 concern the efficacy of the composition after two weeks of use. The responses are organized by panelists who use a concealer product and panelists who do not use a concealer product.

0268Table 12 (Concealer Use-After Two Weeks of Use)

0269Two Weeks After Use - "% Agree' USE QF CONCEALER

0270Use Do Wot Use

0271Concealer Concealer

JL -SL

0273Dark Circles

0274Visibly minimizes appearance of dark circles 72 65 Improv es appearance of dark circles 72 63 Visibly reduces appearance of dark circles 69 62 Diminishes appearance of dark circles 66 63 Softens appearance of dark circles 78 69

0275Softens appearance of undereye puffiness 87 71 Visibly minimizes appearance of un dereye puffiness 81 67 Improv es appearance of undereye puffiness 79 67 Visibly reduces appearance of undereye puffiness 80 63 Diminishes appearance of undereye puffiness 75 62

0276Softens appearance of undereye saggmess 78 59 Improves appearance of undereye saggmess 75 55 Visibly reduces appearance of undereye saggmess 71 52 Visibly minimizes appearance of undereye saggmess 69 50 Diminishes appearance of undereye saggmess 66 43

0277General Appearanπp Of Fye Area Freshens my eye area 91 85 Rejuvenates eye area 82 71 Leaves eye area with a rested look 84 64 Revitalizes eye area 81 62 Leaves eye area looking younger 74 62 Brightens up eye area 75 58 Leaves eye area looking vibrant 72 58

0278[0091] The panelists' responses in Table 13 concern the tactile properties and efficacy of the composition immediately after application to the skin. The responses arc organized by panelists who have dry, normal, and oily skin.

0279Table 13 (Skin Type—Immediately After Application)

0280Immediately After Application ^■ "% Agree"

SKIN TYPE

0282Normal

0283_ΪL

0284I s easy to apply 100 97 96

0285I s lightweight 95 97 100

0286Leaves a soft afterfeel 98 95 96

0287My regular eye cream applies easily over the test product 95 97 95

0288My regular foundation applies easily over the test product 95 97 96

0289Does not feel oily/greasy during application 95 90 93

0290Provides a cooling sensation upon application 98 92 88

0291Does not leave an oily/greasy afterfeel 90 92 91

0292Leaves a smooth afterfeel 88 90 93

0293I s suitable for rny skin type 88 94 89 Has a silky-smooth texture 95 79 86

0294Absorbs quickly 85 82 86

0295Provides a cooling sensation that lasts a few minutes 93 76 77

0296Visibly reduces appearance of undereye puffiness immediately after application 58 44 64

0297Visibly minimizes appearance of undereye puffiness immediately after application 63 39 46

0298Visibly minimizes appearance of dark circles immediately after application 55 35 56

0299Visibly reduces appearance of dark circles immediately after application 51 37 43

0300[0092] The panelists' responses in Table 14 concern the efficacy of the composition after two weeks of use. The responses are organized by panelists who have dry, normal, and oily skin.

0301Table 14 (Skin Type- After Two Weeks of Use)

0302TWo Weeks After Use - "% Agree"

SKIN TYPE

0304Dry Normal Oily

JL JL JL

0306Dark Circles

0307Visibly minimizes appearance of dark circles 63 67 76 Improves appearance of dark circles 68 64 71 Visibly reduces appearance of dark circles 66 58 73 Diminishes appearance of dark circles 66 53 73 Softens appearance of dark circles 68 69 83

0308Softens appearance of undereye puffiness 73 80 87 Visibly minimizes appearance of undereye puffiness 70 77 78 Improves appearance of undereye puffiness 68 80 74 Visibly reduces appearance of undereye puffiness 68 77 73 Diminishes appearance of undereye puffiness 68 71 70

0309Raqqy Skin

0310Softens appearance of undereye sagginess 64 69 11 Improves appearance of undereye sagginess 61 69 69 Visibly reduces appearance of undereye sagginess 58 66 65 Visibly minimizes appearance of undereye sagginess 58 66 58 Diminishes appearance of undereye sagginess 58 51 59

0311General Appearance Of Eye Area Freshens my eye area 85 90 91 Rejuvenates eye area 73 78 81 Leaves eye area with a rested look 73 75 76 Revitalizes eye area 73 74 72 Leaves eye area looking younger 59 68 79 Brightens up eye area 71 61 71 Leaves eye area looking vibrant 63 65 69

0312[0093] The panelists' responses in Table 15 concern the tactile properties and efficacy of the composition immediately after application to the skin. The responses are organized by panelists who have light, medium, and dark skin tones.

0313Table 15 (Skin Tone—Immediately After Application)

0314Immediately After Application - "% Agree"

0315SKIN TONE*

0316Medium Dark

0317I s easy to apply 98 96 100

0318I s lightweight 98 96 100

0319Leaves a soft afterfeel 96 94 100

0320My regular eye cream applies easily over the test product 96 98 91

0321My regular foundation applies easily over the test product 96 98 91

0322Does not feel oily/greasy during application 94 94 88

0323Provides a cooling sensation upon application 96 91 88

0324Does not leave an oily /greasy afterfeel 90 94 88

0325Leaves a smooth afterfeel 88 92 92

0326I s suitable for my skn type 88 94 88

0327Has a silky-smooth texture 85 90 83

0328Absorbs quickly 81 88 83

0329Provides a cooling sensation that lasts a few minutes 86 78 83

0330Visibly reduces appearance of undereye puffiness immediately after application 57 52 61

0331Visibly minimizes appearance of undereye puffiness immediately after application 53 50 42

0332Visibly minimizes appearance of dark circles immediately after application 53 47 46

0333Visibly reduces appearance of dark circles immediately after application 48 41 42

0334'"Light" = Very Light and Light: ''Medium'' = Light to Medium, Medium and Medium to Dark: "Dark" = Dark and Very Dark

0335[0094] The panelists' responses in Table 16 concern the efficacy of the composition after two weeks of use. The responses are organized by panelists who have light, medium, and dark skin tones.

0336Table 16 (Skin Tone- After Two Weeks of Use)

0337Two Weeks After Use - "% Agme' SKIN TONE*

0338Light Medium Dark

0339% %_ %_

0340Dark Circles

0341Visibly minimizes appearance of dark circles 65 67 79 Improves appearance of dark circles 63 71 71 Visibly reduces appearance of dark circles 59 70 71 Diminishes appearance of dark circles 63 63 71 Softens appearance of dark circles 72 73 79

0342Softens appearance of undereye puffiness 71 88 82 Visibly minimizes appearance of undereye puffiness 67 83 73 Improves appearance of undereye puffiness 66 79 77 Visibly reduces appearance of undereye puffiness 66 81 68 Diminishes appearance of undereye puffiness 61 77 71

0343Saggy Skin

0344Softens appearance of undereye sagginεss 67 69 76 Improves appearance of undereye saggmess 67 65 67 Visibly reduces appearance of undereye saggmess 59 62 71 Visibly minimizes appearance of undereye saggmess 62 60 62 Diminishes appearance of undereye saggmess 54 58 57

0345General Appearance Of Eve Area Freshens rny eye area 89 88 88 Rejuvenates eye area 79 78 75 Leaves eye area with a rested look 69 76 83 Revitalizes eye area 71 76 71 Leaves eye area looking younger 60 74 75 Brightens up eye area 60 72 74 Leaves eye area looking vibrant 62 65 75

0346"Liqin" = Very Light ami Liqltt: "Medium^" = Ls'qht to Medium. Medium ami Medium to Dar rkk:: ""DDaarrkk" = Dark ami Very Dark

EXAMPLE 3

0348[0095] Three ingredients that can be used in the compositions of the present invention were tested to determine their effects on melanin production and tyrosinase activity. The three ingredients were CLERILYS™, Ascorbyl Glucoside, and UNINONTAN U34™. The materials and methods used to perform these tests and the corresponding data follows.

0349[0096] Materials and Methods: Human melanocytes from a moderately pigmented donor were purchased from Cascade Biologies (Portland, Oregon). Human dermal fibroblasts were purchased from Cambrex (Rockland, ME). In order to determine the optimal concentrations of the test materials for the whitening experiment, these materials were first examined in a proliferation assay with human dermal fibroblasts. The highest non-interfering concentration was determined for each test material and further 5-fold dilutions were made for use in melanocytes cultures.

0350[0097] The following assay was used to the effects of the materials on melanin production. Melanocytes were seeded in the fully-supplemented 254 medium (Cascade) in 96 well plates and test materials were added 24h later. Cells were grown for 6 days with one growth medium change. At the end of the experiment, cells were lysed with the Cellytic Mammalian Cell Lysis/Extraction Reagent (Sigma, St. Louis, MO) and melanin was solubilized in 2N NaOH. Melanin content was determined spectrophotometrically at 405 nm and standardized to total protein content from the same cultures, using Bradford reagent (Sigma, St. Louis, MO). Kojic acid was used as positive control. Cell cultures were monitored on a Nikon Eclipse inverted microscope.

0351[0098] Tyrosinase activity was measured by the modified Pomerantz method

0352(Biophys Res Commun, 1964). Mushroom Tyrosinase (Sigma #3212816630) stock enzyme was 5 U/well (25 U/ml). The substrate (I-DOPA, Fisher Scientific, Pittsburgh, PA) stock solution was 20 mM. Working solution was prepared in PBS for each assay. The reaction mixture consisted of 50 μl 20 mM I-DOPA and 10 μl undiluted test sample. Reaction was initiated by adding tyrosinase. Assays were preformed in 96 well flat-bottom microtiter plates (Fisher #0720087) and read at 490 nm after 5, 10, and 20 min.

0353[0099] Results: The test results of the melanin production and tyrosinase inhibition assays are summarized in Table 17 below. These results confirm that the tested materials can inhibit melanin production and tyrosinase activity. Table 17 (Melanin Production Assay)

0354<img file="EP2144673A2_D0008.tif" />

0355[00100] All of the compositions and/or methods disclosed and claimed in this specification can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of specific embodiments, it will be apparent to those of skill in the art that variations may be applied to the compositions and/or methods and in the steps or in the sequence of steps of the method described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents which are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.

REFERENCES

0357The following references, to the extent that they provide exemplary procedural or other details supplementary to those set forth herein, are specifically incorporated herein by reference.

0358U.S. Patent 2,798,053 U.S. Patent 3,755,560 U.S. Patent 4,421,769 U.S. Patent 4,509,949 U.S. Patent 4,599,379 U.S. Patent 4,628,078 U.S. Patent 4,835,206 U.S. Patent 4,849,484 U.S. Patent 5,011,681 U.S. Patent 5,084,563 U.S. Patent 5,087,445 U.S. Patent 5,100,660 U.S. Patent 5,252,722 U.S. Patent 5,272,136 U.S. Patent 5,388,420 U.S. Patent 5,432,161 U.S. Patent 5,508,391 U.S. Patent 5,559,146 U.S. Patent 5,720,963 U.S. Patent 5,843,907 U.S. Patent 6,262,541 U.S. Patent 6,290,938 U.S. Patent 6,443,164 U.S. Patent 6,447,760 U.S. Patent 6,482,397 U.S. Patent 6,495,126

0359Bai et al, J. Biol. Chem., 278(37):35501-35507, 2003. Barany and Merrifield, In: The Peptides, Gross and Meienhofer (Eds.), Academic Press, NY,

03601-284, 1979.

0361Biophys. Res. Commun., 16(2): 188-94, 1964. Figlar and Nooteboom, Blumea, 49:87-100, 2004. Houghten et al, Infect. Immun., 48(3):735-740, 1985. International Cosmetic Ingredient Dictionary, lθ' Ed., 2004. McCutcheon's Emulsifiers &amp; Detergents North American Edition, 2001. McCutcheon's Functional Materials North American Edition, 2001. McCutcheon's, Detergents and Emulsifiers, North American Edition, 1986. Merrifield, Science, 232(4748):341-347, 1986. Remington's Pharmaceutical Sciences, 18^th Ed., Mack Printing Company, 1289-1329, 1990.

0362Schiltz et al, J. Investigative Dermatology, 87:663-667, 1986.

0363SStteewwaarrtt aanndd YYoouunngg,, IInn:: SSoolliidd PPhhaassee PPeeppttiiddee SSyynnthesis, 2^nd Ed., Pierce Chemical Co., 1984.

0364Tam et al., J. Am. Chem. Soc, 105:6442, 1983.

0365Vollhardt and Schore, In: Organic Chemistry, 2^nd Ed., W. H. Freeman &amp; Co., 1994.