Nova Patents
EP2025762A3

Heteroduplex tracking assay

Abstract

A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus may be shifted back to CCR5-mediated entry soon after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment and clinical management of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CCR5- or CXCR4-specific strains. The diagnostic methods may be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. The methods of the invention include cell-based methods, including cell fusion assays, and molecular-based methods, including heteroduplex tracking assay, to both quantitatively and qualitatively analyze patient-derived HIV for coreceptor usage.

Term

Projected expiry 17 January 2027.

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Every citation, both ways

Citations
DocumentRelationOfficeCategoryCited duringRelevant claims
US6727060B2CitesUnited States of AmericaYSearch report1-18
NELSON J A E ET AL: "Patterns of changes in human immunodeficiency virus type 1 V3 sequence populations late in infection", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 74, no. 18, 1 September 2000 (2000-09-01), pages 8494 - 8501, XP002490002, ISSN: 0022-538XNon-patent––Search report–
SCHROEDER TAMARA L ET AL: "Characterization of intersubtype recombinant HIV type 1 genomes using a nonradioactive heteroduplex tracking assay.", AIDS RESEARCH AND HUMAN RETROVIRUSES APR 2005, vol. 21, no. 4, April 2005 (2005-04-01), pages 314 - 318, XP002540202, ISSN: 0889-2229Non-patent––Search report–
FREEL S A ET AL: "Characterization of human immunodeficiency virus type 1 in saliva and blood plasma by V3-specific heteroduplex tracking assay and genotype analyses.", JOURNAL OF VIROLOGY MAY 2001, vol. 75, no. 10, May 2001 (2001-05-01), pages 4936 - 4940, XP002540351, ISSN: 0022-538XNon-patent––Search report–
PHILPOTT SEAN M: "HIV-1 coreceptor usage, transmission, and disease progression", CURRENT HIV RESEARCH, BENTHAM SCIENCE PUBLISHERS, HILVERSUM, NL, vol. 1, no. 2, 1 April 2003 (2003-04-01), pages 217 - 227, XP009118392, ISSN: 1570-162XNon-patent––Search report–
WOLINSKY S M ET AL: "Effect of a CCR5 inhibitor on viral loads in macaques dual-infected with R5 and X4 primate immunodeficiency viruses", VIROLOGY, ACADEMIC PRESS,ORLANDO, US, vol. 328, no. 1, 15 October 2004 (2004-10-15), pages 19 - 29, XP004563061, ISSN: 0042-6822Non-patent––Search report–
WEISER BARBARA ET AL: "HIV-1 coreceptor usage and CXCR4-specific viral load predict clinical disease progression during combination antiretroviral therapy", AIDS, LONDON, GB, vol. 22, no. 4, 19 February 2008 (2008-02-19), pages 469 - 479, XP002490004, ISSN: 0269-9370Non-patent––Search report–