Labelled nucleotides

FIELD OF THE INVENTION

0001This invention relates to labelled nucleotides. In particular, this invention discloses nucleotides having a removable detectable label and their use in polynucleotide sequencing methods.

BACKGROUND

0002Advances in the study of molecules have been led, in part, by improvement in technologies used to characterise the molecules or their biological reactions. In particular, the study of the nucleic acids DNA and RNA has benefited from developing technologies used for sequence analysis and the study of hybridisation events.

0003An example of the technologies that have improved the study of nucleic acids, is the development of fabricated arrays of immobilised nucleic acids. These arrays consist typically of a high-density matrix of polynucleotides immobilised onto a solid support material. See, <i>e.g.,</i><nplcit id="ncit0001" npl-type="s"><text>Fodor et al., Trends Biotech. 12:19-26, 1994</text></nplcit>, which describes ways of assembling the nucleic acids using a chemically sensitized glass surface protected by a mask, but exposed at defined areas to allow attachment of suitably modified nucleotide phosphoramidites. Fabricated arrays can also be manufactured by the technique of "spotting" known polynucleotides onto a solid support at predetermined positions (<i>e.g</i>., <nplcit id="ncit0002" npl-type="s"><text>Stimpson et al., Proc. Natl. Acad. Sci. USA 92:6379-6383, 1995</text></nplcit>).

0004A further development in array technology is the attachment of the polynucleotides to the solid support material to form single molecule arrays. Arrays of this type are disclosed in International Patent App. <patcit id="pcit0001" dnum="WO0006770A"><text>WO 00/06770</text></patcit>. The advantage of these arrays is that reactions can be monitored at the single molecule level and information on large numbers of single molecules can be collated from a single reaction.

0005For DNA arrays to be useful, the sequences of the molecules must be determined. <patcit id="pcit0002" dnum="US5302509A"><text>U.S. Pat. No. 5,302,509</text></patcit> discloses a method to sequence polynucleotides immobilised on a solid support. The method relies on the incorporation of 3'-blocked bases A, G, C and T, each of which has a distinct fluorescent label, into the immobilised polynucleotide, in the presence of DNA polymerase. The polymerase incorporates a base complementary to the target polynucleotide, but is prevented from further addition by the 3'-blocking group. The label of the incorporated base can then be determined and the blocking group removed by chemical cleavage to allow further polymerisation to occur.

0006<nplcit id="ncit0003" npl-type="s"><text>Welch et al. (Chem. Eur. J. 5 (3) :951-960, 1999</text></nplcit>) describes the synthesis of nucleotide triphosphates modified with a 3'-O-blocking group that is photolabile and fluorescent. The modified nucleotides are intended for use in DNA sequencing experiments. However, these nucleotides proved to be difficult to incorporate onto an existing polynucleotide, due to an inability to fit into the polymerase enzyme active site.

0007<nplcit id="ncit0004" npl-type="s"><text>Zhu et al. (Cytometry 28:206-211, 1997</text></nplcit>) also discloses the use of fluorescent labels attached to a nucleotide via the base group. The labelled nucleotides are intended for use in fluorescence <i>in situ</i> hybridisation (FISH) experiments, where a series of incorporated labelled nucleotides is required to produce a fluorescent "bar code".

0008<patcit id="pcit0003" dnum="WO995732A"><text>WO99/5732</text></patcit> describes the use of nucleotides comprising fluorophores linked to the nucleotide by chemically or photochemically cleavable linker moieties.

0009<patcit id="pcit0004" dnum="WO0053812A"><text>WO00/53812</text></patcit> and <patcit id="pcit0005" dnum="EP1291354A2"><text>EP-A2-1 291 354</text></patcit> disclose nucleotide compounds of general structure FluorophoreS-S-Linker-Nucleotide and their use in nucleic acid assay methods. <patcit id="pcit0006" dnum="WO0053812A"><text>WO00/53812</text></patcit> also makes reference to periodate cleavage of a cis-glycol linkage between nucleotide and fluorophore.

0010<patcit id="pcit0007" dnum="WO0192284A"><text>WO 01/92284</text></patcit> discloses the use of enzyme-cleavable groups linking blocking and reporting moieties to nucleotides. It is preferred that these enzyme-cleavable groups are the same, i.e. that both the blocking and reporter moieties are attached to the nucleotide by a chain comprising a group cleavable by a common enzyme. Cleavable groups described in <patcit id="pcit0008" dnum="WO0192284A"><text>WO 01/92284</text></patcit> are esters and amides, cleavable by esterases and amidases respectively.

0011<patcit id="pcit0009" dnum="WO02290A"><text>WO02/290</text></patcit> describes nucleotides analogues that contain a label linked through cleavable linkers to the nucleotide base, or an analogue of the base. Photocleavable linkers comprising 2-nitrobenzyl moieties are described.

SUMMARY OF THE INVENTION

0012The present inventors have surprisingly found that certain linkers which connect the bases of nucleotides to detachable labels, e.g. fluorophores, may be cleaved using water-soluble phosphines or water-soluble transition metal catalysts formed from a transition metal and at least partially water-soluble ligands. In aqueous solution the latter form at least partially water-soluble transition metal complexes.

0013Labelled nucleotides comprising such linkers an methods for using them are clearly advantageous in the context of techniques carried out in aqueous media such as sequencing reactions, polynucleotide synthesis, nucleic acid amplification, nucleic acid hybridization assays, single nucleotide polymorphism studies, and other techniques using enzymes such as polymerases, reverse transcriptases, terminal transferases, or other DNA modifying enzymes. The invention is especially useful in techniques that use labelled dNTPs, such as nick translation, random primer labeling, end-labeling (<i>e.g</i>., with terminal deoxynucleotidyltransferase), reverse transcription, or nucleic acid amplification.

0014According to a first aspect of the invention, there is provided a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: <chemistry id="chem0001" num="0001"><img file="EP1560838B1_D0001.tif" /></chemistry><chemistry id="chem0002" num="0002"><img file="EP1560838B1_D0002.tif" /></chemistry> (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C_1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C_1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).

0015According to a second aspect of the invention, there is provided a method of cleaving a linker that contains a moiety selected from the groups comprising: <chemistry id="chem0003" num="0003"><img file="EP1560838B1_D0003.tif" /></chemistry> (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C_1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C_1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of a nucleotide or nucleoside), said linker being present in the nucleotide or nucleoside and connecting the base thereof to a detectable label, said method comprising contacting the nucleotide or nucleoside with a water-soluble phosphine-based transition metal catalyst.

0016According to a third aspect of the invention, there is provided a method of cleaving a linker that contains a moiety selected from the groups comprising: <chemistry id="chem0004" num="0004"><img file="EP1560838B1_D0004.tif" /></chemistry> (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C_1-10 substituted or unsubstituted alkyl group, T is hydrogen or a C_1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of a nucleotide or nucleoside), said linker being present in the nucleotide or nucleoside and connecting the base thereof to a detectable label, said method comprising contacting the nucleotide or nucleoside with a water-soluble phosphine.

0017The method according to the second and third aspects of the invention are particularly useful in sequencing reactions. Such reactions constitute a further aspect of the invention. Viewed from this aspect, the invention provides a method for determining an identity of a nucleotide in a target single-stranded polynucleotide, comprising: <ol id="ol0001" compact="compact"><li>(a) providing one or more of the nucleotides A, G, C and T or U in which each of said nucleotides has a base that is attached to a distinct detectable label via a linker according to the first aspect of the invention, said linker being cleavable with a water-soluble phosphine; and a nascent polynucleotide complementary to the target polynucleotide, one of said provided nucleotides being suitable for incorporation into said nascent polynucleotide;</li><li>(b) incorporating the nucleotide suitable for incorporation into said nascent polynucleotide; and</li><li>(c) carrying out a method according to the second or third aspect of the invention.</li></ol>

DESCRIPTION OF THE DRAWINGS

0018<ul id="ul0001" list-style="none" compact="compact"><li><figref idref="f0001">Fig. 1</figref> shows exemplary nucleotide structures useful in the invention. For each structure, X can be H, phosphate, diphosphate or triphosphate. R₁ and R₂ can be the same or different, and can be selected from H, OH, or any group which can be transformed into an OH.</li><li><figref idref="f0002">Fig. 2</figref> shows some functional molecules useful in the invention, which include some cleavable linkers. In these structures, R₁ and R₂ may be the same or different, and can be H, OH, or any group which can be transformed into an OH group, including a carbonyl. R₃ represents one or more substituents independently selected from alkyl, alkoxyl, amino or halogen groups. Alternatively, cleavable linkers may be constructed from any labile functionality used on the 3'-block.</li><li><figref idref="f0003">Fig. 3</figref> is a schematic illustration of some of the 2' or 3' OH blocking groups which can be present in the nucleotides or nucleosides according to the invention.</li><li><figref idref="f0004">Fig. 4</figref> shows two cycles of incorporation of a fully functional T nucleoside triphosphate against a poly A template</li></ul>

DETAILED DESCRIPTION

0019The nucleotide or nucleoside molecules of the invention each have a base that is linked to a detectable label via linkers that may be cleaved by contact with water-soluble phosphines or water-soluble transition metal-containing catalysts described in greater detail hereinafter. Preferably moiety "T" is hydrogen. The base can be a purine, or a pyrimidine. The base can be a deazapurine. The molecule can have a ribose or deoxyribose sugar moiety. The ribose or deoxyribose sugar can include a protecting group attached via the 2' or 3' oxygen atom. The protecting group can be removed to expose a 3'-OH. The molecule can be a deoxyribonucleotide triphosphate. The detectable label can be a fluorophore.

0020The invention also embraces oligonucleotides which comprise one or more nucleotides of the invention. Preferably, at least one nucleotide of the invention is present at a terminal position in such aoligonucleotide.

0021The linker may be attached to the 5-position in pyrimidines or the 7-position in purines or deazapurines. The characteristic feature of the nucleotides and nucleosides of the present invention is the amenability of the linkage to cleavage by certain water-soluble phosphines or phosphine-based transition metal catalysts. Since oligonucleotides are manipulated in aqueous solution, the advantages of this water-solubility are evident.

0022The cleavable linkages present in the nucleosides and nucleotides of the invention each comprise an allyl or azido group.

0023Where the linkers comprise an azide-containing group the linkers may contain a moiety of the formula: <chemistry id="chem0005" num="0005"><img file="EP1560838B1_D0005.tif" /></chemistry>

0024These moieties may be present in either orientation in the linker connecting the base of the nucleotide/nucleoside with the detectable label, that is to say either of the bonds shown terminating in asterisks in each moiety may be closer to the base (or the label) in each nucleotide or nucleoside than the other asterisk shown in each structure. Thus the invention embraces nucleotides and nucleoside having schematically the following structures (shown on the left-hand side) which may be reacted with the water-soluble phosphines (described in greater detail hereinafter) to generate the products shown on the right-hand side in which the azido-containing linker has been cleaved: <chemistry id="chem0006" num="0006"><img file="EP1560838B1_D0006.tif" /></chemistry><chemistry id="chem0007" num="0007"><img file="EP1560838B1_D0007.tif" /></chemistry><chemistry id="chem0008" num="0008"><img file="EP1560838B1_D0008.tif" /></chemistry><chemistry id="chem0009" num="0009"><img file="EP1560838B1_D0009.tif" /></chemistry>

0025Whilst the connecting points * indicate the points at which the moieties are connected to the nucleotide or nucleoside, it will be appreciated that these points are generally the points at which the moiety is connected to the remainder of the linker. In other words, the moieties described herein that contain allyl or azido groups are generally part of, rather than exclusively constitute, the cleavable linker.

0026Where the moiety is of formula -X-CH(N₃)-, the nature of the substituents to either side of the moiety -X-CH(N3)- affects the stability of the moiety and thus the rate at which it is cleaved. For example, where the linkage contains a substituted aryl group attached to the moiety in which the substituents (indicated as "R" in the structures immediately below) are electron-withdrawing, this is manifested in the way in which the moiety cleaves. For example, electron-withdrawing groups, serve to stabilise the linkage particularly where X is O or S. This makes cleavage occur more slowly.

0027Shown below schematically are the outcomes of four cleavages of schematic nucleotide constructs and a further schematic representation of a preferred construct. The dotted lines connecting the "fluor", "dNTP" or R group to the benzene ring or the cleavable moieties indicate that substitution may be at any free position on the benzene ring and that further atoms (not shown) in the linker may be present between the cleavable motif shown and the nucleotide and detectable label which the linker connects: <chemistry id="chem0010" num="0010"><img file="EP1560838B1_D0010.tif" /></chemistry><chemistry id="chem0011" num="0011"><img file="EP1560838B1_D0011.tif" /></chemistry><chemistry id="chem0012" num="0012"><img file="EP1560838B1_D0012.tif" /></chemistry><chemistry id="chem0013" num="0013"><img file="EP1560838B1_D0013.tif" /></chemistry><chemistry id="chem0014" num="0014"><img file="EP1560838B1_D0014.tif" /></chemistry>

0028Where the azide-containing moieties are Sieber linkers, i.e. are of the fomula <chemistry id="chem0015" num="0015"><img file="EP1560838B1_D0015.tif" /></chemistry> cleavage of the moiety takes place across the bond linking the central 6-membered ring of the tricycle to the amide such that a terminal amide group is left pendant to either the base or the fluorophore after cleavage.

0029It will be appreciated that the azide-containing Sieber linker moieties may contain one or more substituents which may be either electron-donating (examples include alkyl or alkoxy, e.g. C_1-6 alkyl or C_1-6 alkoxy groups) or electron-withdrawing groups (e.g. nitro, cyano, fluoro etc). Introduction of such substituents enables the skilled person to tailor the conditions under which which cleavage may be effected.

0030Where the nucleotides comprise an azide group in the linker, this may be converted to the primary amine group with a thiol (in place of the phosphines), preferably a water-soluble thiol such as dithiothreitol (DTT).

0031Where the linkers comprise an allyl group, these may be of the formulae: <chemistry id="chem0016" num="0016"><img file="EP1560838B1_D0016.tif" /></chemistry>

0032As with the azido-containing moieties discussed above, these linking moieties may be present in either orientation in the linker connecting the base of the nucleotide/nucleoside with the detectable label.

0033Where the linkages comprise allyl-containing moieties, these linkers may be cleaved with water-soluble transition metal catalysts formed from a transition metal and at least partially water-soluble ligands. In aqueous solution these form at least partially water-soluble transition metal complexes.

0034The transition metal species serves to remove the allyl group. Where the allyl group is part of carbamate, deallylation affords the corresponding carbamic acid. This spontaneously decarboxylates to afford a hemiaminal which hydrolyses so as to effect cleavage of the linker. Corresponding chemistries operate with the analogous thiocarbamate and carbonates so as to generate compounds containing moieties of structure *-C(NH₂)-X-*. These collapse by hydrolysing, again cleaving the linker.

0035By aqueous solution herein is meant a liquid comprising at least 20 vol%, preferably at least 50%, for example at least 75 vol%, particularly at least 95 vol% and especially greater than above 98 vol%, ideally 100 vol% of water as the continuous phase.

0036Transition metals of use in forming the catalysts described herein are preferably platinum, palladium, rhodium, ruthenium, osmium and iridium. Palladium is particularly preferred.

0037The transition metal, e.g. palladium, is conveniently introduced as a salt, e.g. as a halide. Mixed salts such as Na₂PdCl₄ may also be used. Other appropriate salts and compounds will be readily determined by the skilled person and are commercially available, e.g. from Aldrich Chemical Company.

0038Suitable phosphines are any phosphine or mixed nitrogen-phosphine ligands known to those skilled in the art, characterised in that the ligands are derivatised so as to render them water-soluble, e.g. by introducing one or more sulfonate, amine, hydroxyl (preferably a plurality of hydroxyl) or carboxylate residues. Where amine residues are present, formation of amine salts may assist the solublisation of the ligand and thus the metal-allyl complex. Examples of appropriate ligands are triaryl phosphines, e.g. triphenyl phosphine, derivatised so as to make them water-soluble. Also preferred are trialkyl phosphines, e.g. tri-C_1-6-alkyl phosphines such as triethyl phosphines; such trialkyl phosphines are likewise derivatised so as to make them water-soluble. Sulfonate-containing and carboxylate-containing phosphines are particularly preferred; an example of the former 3,3',3"-phosphinidynetris (benzenesulfonic acid) which is commercially available from Aldrich Chemical Company as the trisodium salt; and a preferred example of the latter is <i>tris</i>(2-carboxyethyl)phosphine which is available from Aldrich as the hydrochloride salt. The derivatised water-soluble phosphines and nitrogen-containing phosphines described herein may be used as their salts (e.g. as the hydrochloride or sodium salts) or, for example, in the case of the sulfonic and carboxylic acid-containing phosphines described herein, as the free acids. Thus 3,3',3"-phosphinidynetris (benzenesulfonic acid) and <i>tris</i>(2-carboxyethyl)phosphines may be introduced either as the triacids or the trisodium salts. Other appropriate salts will be evident to those skilled in the art. The existence in salt form is not particularly important provided the phosphines are soluble in aqueous solution.

0039Other phosphines which may be used include the following: <chemistry id="chem0017" num="0017"><img file="EP1560838B1_D0017.tif" /></chemistry><chemistry id="chem0018" num="0018"><img file="EP1560838B1_D0018.tif" /></chemistry><chemistry id="chem0019" num="0019"><img file="EP1560838B1_D0019.tif" /></chemistry><chemistry id="chem0020" num="0020"><img file="EP1560838B1_D0020.tif" /></chemistry><chemistry id="chem0021" num="0021"><img file="EP1560838B1_D0021.tif" /></chemistry>

0040The skilled person will be aware that the atoms chelated to the transition metal in the water soluble complex may be part of mono- or polydentate ligands. Some such polydentate ligands are shown above. Whilst monodentate ligands are preferred, the invention thus also embraces methods which use water-soluble bi-, tri-, tetra-, penta- and hexadentate water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands.

0041As noted earlier, the aqueous solution in which deprotection is effected need not be 100% (as the continuous phase). However, substantially pure water (e.g. at least 98 vol% and preferably about 100 vol%) is preferred. Cosolvents are generally not required. Generally, nucleotides and nucleosides are readily soluble in water (e.g. pure water) in which the linkage cleavage described herein may be effected. If desirable, one or more water-miscible cosolvents may be employed. Appropriate solvents include acetonitrile or dimethylsulfoxide, methanol, ethanol and acetone, methanol being preferred. Less preferred solvents include tetrahydrofuran (THF) and dioxane.

0042In the methods of cleaving allyl-containing moieties according to the invention, a soluble metal complex is formed comprising a transition metal and one or more water-soluble phosphine ligands (including water-soluble nitrogen-containing phosphine ligands). More than one type of water-soluble phosphine/nitrogen-containing phosphine ligand may be used in any given reaction although generally only one type of these classes of ligand will be used in any given catalyst. The quantity of transition metal, e.g. palladium, may be less than 1 mol% (calculated relative to the number of moles of linkage to be cleaved). Advantageously the amount of catalyst may be much less than 1 mol%, e.g. &lt;0.50 mol%, preferably &lt;0.10 mol%, particularly &lt;0.05mol%. Even lower quantities of metal may be used, for example &lt;0.03 or even &lt;0.01 mol%. As those skilled in the art will be aware, however, as quantity of catalyst is reduced, so too is the speed of the reaction. The skilled person will be able to judge, in any instance, an appropriate quantity of transition metal and thus catalyst suitable for any particular reaction.

0043In contrast to the amount of metal required in forming the active catalyst, the quantity of water-soluble phosphine-containing ligand(s) used is preferably be greater than 1 molar equivalent (again calculated relative to the number of moles of linkage to be cleaved). Preferably greater than 4, e.g. greater than 6, for example 8-12 molar equivalents of ligand may be used. Even higher quantities of ligand e.g. >20 mole equivalents may be used if desired.

0044The skilled person will be able to determine the quantity of ligand best suited to any individual reaction.

0045Where the linkage contains an azide group, the presence of a transition metal is not necessary to effect cleavage. Thus cleavage of such linkers may be effected in the presence only of the phosphines discussed herein; these may be present in the methods of the invention as water-soluble salts, such as those discussed herein.

0046As is known in the art, a "nucleotide" consists of a nitrogenous base, a sugar, and one or more phosphate groups. In RNA, the sugar is a ribose, and in DNA is a deoxyribose, <i>i.e.,</i> a sugar lacking a hydroxyl group that is present in ribose. The nitrogenous base is a derivative of purine or pyrimidine. The purines are adenosine (A) and guanidine (G), and the pyrimidines are cytidine (C) and thymidine (T) (or in the context of RNA, uracil (U)). The C-1 atom of deoxyribose is bonded to N-1 of a pyrimidine or N-9 of a purine. A nucleotide is also a phosphate ester of a nucleoside, with esterification occurring on the hydroxyl group attached to C-5 of the sugar. Nucleotides are usually mono, di- or triphosphates.

0047A "nucleoside" is structurally similar to a nucleotide, but is missing the phosphate moieties. An example of a nucleoside analog would be one in which the label is linked to the base and there is no phosphate group attached to the sugar molecule.

0048Although the base is usually referred to as a purine or pyrimidine, the skilled person will appreciate that derivatives and analogs are available which do not alter the capability of the nucleotide or nucleoside to undergo Watson-Crick base pairing. "Derivative" or "analog" means a compound or molecule whose core structure is the same as, or closely resembles that of, a parent compound, but which has a chemical or physical modification, such as a different or additional side group, which allows the derivative nucleotide or nucleoside to be linked to another molecule. For example, the base can be a deazapurine.

0049The modified nucleotides or nucleotides disclosed and claimed herein are examples of such derivatives or analogues with the addition of detectable labels connected to the bases by the cleavable allyl- or azido-containing linkers described herein. Thus the terms nucleotides and nucleosides as used herein will be understood to embrace such modified nucleosides and nucleotides.

0050The derivatives should be capable of undergoing Watson-Crick pairing. "Derivative" and "analogue" also mean a synthetic nucleotide or nucleoside derivative having modified base moieties and/or modified sugar moieties. Such derivatives and analogs are discussed in, e.g., <nplcit id="ncit0005" npl-type="b"><text>Scheit, Nucleotide Analogs (John Wiley &amp; Son, 1980</text></nplcit>) and <nplcit id="ncit0006" npl-type="s"><text>Uhlman et al., Chemical Reviews 90:543-584, 1990</text></nplcit>. Nucleotide analogs can also comprise modified phosphodiester linkages, including phosphorothioate, phosphorodithioate, alkyl-phosphonate, phosphoranilidate and phosphoramidate linkages. The analogs should be capable of undergoing Watson-Crick base pairing. "Derivative", "analog" and "modified" as used herein, may be used interchangeably, and are encompassed by the terms "nucleotide" and "nucleoside" defined herein.

0051The methods of the present invention make use of conventional detectable labels. Detection can be carried out by any suitable method, including fluorescence spectroscopy or by other optical means. The preferred label is a fluorophore, which, after absorption of energy, emits radiation at a defined wavelength. Many suitable fluorescent labels are known. For example, <nplcit id="ncit0007" npl-type="s"><text>Welch et al. (Chem. Eur. J. 5(3):951-960, 1999</text></nplcit>) discloses dansyl-functionalised fluorescent moieties that can be used in the present invention. <nplcit id="ncit0008" npl-type="s"><text>Zhu et al. (Cytometry 28:206-211, 1997</text></nplcit>) describes the use of the fluorescent labels Cy3 and Cy5, which can also be used in the present invention. Labels suitable for use are also disclosed in <nplcit id="ncit0009" npl-type="s"><text>Prober et al. (Science 238:336-341, 1987</text></nplcit>); <nplcit id="ncit0010" npl-type="s"><text>Connell et al. (BioTechniques 5(4):342-384, 1987</text></nplcit>), <nplcit id="ncit0011" npl-type="s"><text>Ansorge et al. (Nucl. Acids Res. 15(11):4593-4602, 1987</text></nplcit>) and <nplcit id="ncit0012" npl-type="s"><text>Smith et al. (Nature 321:674, 1986</text></nplcit>). Other commercially available fluorescent labels include, but are not limited to, fluorescein, rhodamine (including TMR, Texas red and Rox), alexa, bodipy, acridine, coumarin, pyrene, benzanthracene and the cyanins.

0052Multiple labels can also be used in the invention. For example, bi-fluorophore FRET cassettes (<nplcit id="ncit0013" npl-type="s"><text>Tet. Letts. 46:8867-8871, 2000</text></nplcit>) are well known in the art and can be utilised in the present invention. Multi-fluor dendrimeric systems (<nplcit id="ncit0014" npl-type="s"><text>J. Amer. Chem. Soc. 123:8101-8108, 2001</text></nplcit>) can also be used.

0053Although fluorescent labels are preferred, other forms of detectable labels will be apparent as useful to those of ordinary skill. For example, microparticles, including quantum dots (<nplcit id="ncit0015" npl-type="s"><text>Empodocles, et al., Nature 399:126-130, 1999</text></nplcit>), gold nanoparticles (<nplcit id="ncit0016" npl-type="s"><text>Reichert et al., Anal. Chem. 72:6025-6029, 2000</text></nplcit>), microbeads (<nplcit id="ncit0017" npl-type="s"><text>Lacoste et al., Proc. Natl. Acad. Sci USA 97(17):9461-9466, 2000</text></nplcit>), and tags detectable by mass spectrometry can all be used.

0054Multi-component labels can also be used in the invention. A multi-component label is one which is dependent on the interaction with a further compound for detection. The most common multi-component label used in biology is the biotin-streptavidin system. Biotin is used as the label attached to the nucleotide base. Streptavidin is then added separately to enable detection to occur. Other multi-component systems are available. For example, dinitrophenol has a commercially available fluorescent antibody that can be used for detection.

0055The label (or label and linker construct) can be of a size or structure sufficient to act as a block to the incorporation of a further nucleotide onto the nucleotide of the invention. This permits controlled polymerization to be carried out. The block can be due to steric hindrance, or can be due to a combination of size, charge and structure.

0056The invention will be further described primarily with reference to nucleotides. However, unless indicated otherwise, the references herein to nucleotides are also intended to be applicable to nucleosides. The invention will also be further described with reference to DNA, although the description will also be applicable to RNA, PNA, and other nucleic acids, unless otherwise indicated.

0057The modified nucleotides of the invention use a cleavable linker to attach the label to the nucleotide. The use of a cleavable linker ensures that the label can, if required, be removed after detection, avoiding any interfering signal with any labelled nucleotide incorporated subsequently.

0058The use of the term "cleavable linker" is not meant to imply that the whole linker is required to be removed from the nucleotide base. The cleavage site can be located at a position on the linker that ensures that part of the linker remains attached to the nucleotide base after cleavage.

0059The linker can be attached at any position on the nucleotide base provided that Watson-Crick base pairing can still be carried out. In the context of purine bases, it is preferred if the linker is attached via the 7-position of the purine or the preferred deazapurine analogue, via an 8-modified purine, via an N-6 modified adenosine or an N-2 modified guanine. For pyrimidines, attachment is preferably via the 5-position on cytidine, thymidine or uracil and the N-4 position on cytosine. Suitable nucleotide structures are shown in <figref idref="f0001">Fig. 1</figref>. For each structure in <figref idref="f0001">Fig. 1</figref>, X can be H, phosphate, diphosphate or triphosphate. R₁ and R₂ can be the same or different, and can be selected from H, OH, or any group which can be transformed into an OH, including, but not limited to, a carbonyl.

0060Suitable linkers comprise the azide- and allyl-containing moieties discussed earlier. However, in addition to these cleavable moieties, other cleavable motifs may of course also be present in the linkers. Referring to <figref idref="f0002">Fig.2</figref>, examples of these include, but are not limited to, disulfide linkers (1), acid labile moieties (2, 3, 4 and 5; including dialkoxybenzyl moieties (<i>e.g</i>., 2), Sieber linkers (<i>e.g</i>., 3), indole moieties (<i>e.g</i>., 4), t-butyl Sieber moieties (<i>e.g</i>., 5)), electrophilically cleavable moieties, nucleophilically cleavable moieties, photocleavable moieties, cleavage under reductive conditions, oxidative conditions, cleavage via use of safety-catch moieties, and cleavage by elimination mechanisms. Examples of such moieties are described in <patcit id="pcit0010" dnum="WO03048387A"><text>WO03/048387</text></patcit>.

0061As well as the moiety cleavable by water-soluble phosphines or transition metal-based catalysts described herein, the cleavable linkages may also comprise a spacer unit. The spacer distances the nucleotide base from the cleavage site or label. The length of the moiety is unimportant provided that the label is held a sufficient distance from the nucleotide so as not to interfere with any interaction between the nucleotide and an enzyme.

0062Such spacing groups may contain one or more arylene, e.g. phenylene, groups in the chain (i.e. a moiety -Ar- where the phenyl ring is part of the linker by way of its 1,4, 1,3 or 1,2-disposed carbon atoms). The phenyl ring may be substituted at its non-bonded position with one or more substituents such as alkyl, hydroxyl, alkyloxy, halide, nitro, carboxyl or cyano and the like, particularly electron-withdrawing groups, which electron-withdrawing is either by induction or resonance. An example of an electron-withdrawing group by resonance is nitro; a group which acts through induction is fluoro. The skilled person will be aware of other appropriate electron-withdrawing groups. The linkage in the R' group may also include moieties such a -O-, -S(O)_q, wherein q is 0, 1 or 2 or NH or Nalkyl.

0063The modified nucleotides can also comprise additional groups or modifications to the sugar group. For example, a dideoxyribose derivative, lacking both hydroxyl groups on the ribose ring structure (at the 2' and 3' positions), can be prepared and used as a block to further nucleotide incorporation on a growing oligonucleotide strand. The protecting group is intended to prevent nucleotide incorporation onto a nascent polynucleotide strand, and can be removed under defined conditions to allow polymerisation to occur. In contrast to the prior art, there need be no detectable label attached at the ribose 3' position. This allows that steric hindrance with the polymerase enzyme to be reduced, while still allowing control of incorporation using the protecting group.

0064The skilled person will appreciate how to attach a suitable protecting group to the ribose ring to block interactions with the 3'-OH. The protecting group can be attached directly at the 3' position, or can be attached at the 2' position (the protecting group being of sufficient size or charge to block interactions at the 3' position). Alternatively, the protecting group can be attached at both the 3' and 2' positions, and can be cleaved to expose the 3'OH group.

0065Suitable protecting groups will be apparent to the skilled person, and can be formed from any suitable protecting group disclosed in "<nplcit id="ncit0018" npl-type="b"><text>Protective Groups in Organic Synthesis", T.W.Greene and P.G.M. Wuts, 3rd Ed., Wiley Interscience, New York</text></nplcit>. The protecting group should be removable (or modifiable) to produce a 3' OH group. The process used to obtain the 3' OH group can be any suitable chemical or enzymic reaction.

0066Preferably, the blocking, or protecting group is an allyl group or a group of the structure         -O-Z wherein Z is any of -C(R')₂-O-R", -C(R')₂-N(R")₂,-C(R')₂-N(H)R", -C(R')₂-S-R" and -C(R')₂-F, wherein each R" is or is part of a removable protecting group; each R' is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group; or (R')₂ represents an alkylidene group of formula =C(R''')₂ wherein each R''' may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R" is exchanged for H or, where Z is -C(R')₂-F, the F is exchanged for OH, SH or NH₂, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3'OH; with the proviso that where Z is -C(R')₂-S-R", both R' groups are not H.

0067Where the blocking group is an allyl group, it may be introduced into the 3'-position using standard literature procedures such as that used by <nplcit id="ncit0019" npl-type="s"><text>Metzker (Nucleic Acids Research, 22(20):4259-4267, 1994</text></nplcit>).

0068The intermediates produced advantageously spontaneously dissociate under aqueous conditions back to the natural 3' hydroxy structure, which permits further incorporation of another nucleotide. Any appropriate protecting group may be used. Preferably, Z is of formula -C(R')₂-O-R", -C(R')₂-N(R")₂, -C(R')₂-N(H)R" and -C(R')₂-SR". Particularly preferably, Z is of the formula -C(R')₂-O-R", -C(R')₂-N(R")₂, and-C(R')₂-SR". R" may be a benzyl group or a substituted benzyl group.

0069One example of groups of structure -O-Z wherein Z is -C(R')₂-N(R")₂ are those in which -N(R")₂ is azido (-N₃). One preferred such example is azidomethyl wherein each R' is H. Alternatively, R' in Z groups of formula -C(R')₂-N₃ and other Z groups may be any of the other groups discussed herein. Examples of typical R' groups include C_1-6 alkyl, particularly methyl and ethyl, and the following (in which each structure shows the bond which connects the R' moiety to the carbon atom to which it is attached in the Z groups; the asterisks (*) indicate the points of attachment): <chemistry id="chem0022" num="0022"><img file="EP1560838B1_D0022.tif" /></chemistry><chemistry id="chem0023" num="0023"><img file="EP1560838B1_D0023.tif" /></chemistry><chemistry id="chem0024" num="0024"><img file="EP1560838B1_D0024.tif" /></chemistry> (wherein each R is an optionally substituted C_1-10 alkyl group, an optionally substituted alkoxy group, a halogen atom or functional group such as hydroxyl, amino, cyano, nitro, carboxyl and the like) and "Het" is a heterocyclic (which may for example be a heteroaryl group). These R' groups shown above are preferred where the other R' group is the same as the first or is hydrogen. Preferred Z groups are of formula C(R')₂N₃ in which the R' groups are selected from the structures given above and hydrogen; or in which (R')₂ represents an alkylidene group of formula =C(R'")₂, e.g. =C(Me)₂.

0070Where molecules contain Z groups of formula C(R')₂N₃, the azido group may be converted to amino by contacting such molecules with the phosphine or nitrogen-containing phosphines ligands described in detail in connection with the transition metal complexes which serve to cleave the allyl groups from compounds of formula PN-O-allyl, formula R-O-allyl, R₂N(allyl), RNH(allyl), RN(allyl)₂ and R-S-allyl. When transforming azido to amino, however, no transition metal is necessary. Alternatively, the azido group in Z groups of formula C(R')₂N₃ may be converted to amino by contacting such molecules with the thiols, in particular water-soluble thiols such as dithiothreitol (DTT).

0071The labile linker may, and preferably does, consist of functionality cleavable under identical conditions to the block. This makes the deprotection process more efficient since only a single treatment will be required to cleave both the label and the block. For example, where the linkage contains an allyl moiety as discussed and claimed herein and the blocking group is an allyl group, both linkage and blocking group will be cleavable under identical conditions. Similarly, if the linkage contains an azido moiety as discussed and claimed herein and the blocking group comprises an azido moiety, e.g. is of formula Z wherein R" is N₃ as discussed hereinbefore, both linkage and blocking group will be cleavable under identical conditions. The blocking group may of course be deprotected under entirely different chemical conditions to those required to cleave the linker.

0072The term "alkyl" covers both straight chain and branched chain alkyl groups. Unless the context indicates otherwise, the term "alkyl" refers to groups having 1 to 10 carbon atoms, for example 1 to 8 carbon atoms, and typically from 1 to 6 carbon atoms, for example from 1 to 4 carbon atoms. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, n-pentyl, 2-pentyl, 3-pentyl, 2-methyl butyl, 3-methyl butyl, and n-hexyl and its isomers.

0073Examples of cycloalkyl groups are those having from 3 to 10 ring atoms, particular examples including those derived from cyclopropane, cyclobutane, cyclopentane, cyclohexane and cycloheptane, bicycloheptane and decalin.

0074Where alkyl (including cycloalkyl) groups are substituted, particularly where these form either both of the R' groups of the molecules of the invention, examples of appropriate substituents include halogen substituents or functional groups such as hydroxyl, amino, cyano, nitro, carboxyl and the like. Such groups may also be substituents, where appropriate, of the other R' groups in the molecules of the invention.

0075Examples of alkenyl groups include, but are not limited to, ethenyl (vinyl), 1-propenyl, 2-propenyl (allyl), isopropenyl, butenyl, buta-1,4-dienyl, pentenyl, and hexenyl .

0076Examples of cycloalkenyl groups include, but are not limited to, cyclopropenyl, cyclobutenyl, cyclopentehyl, cyclopentadienyl and cyclohexenyl.

0077The term alkoxy refers to C_1-6 alkoxy unless otherwise indicated: -OR, wherein R is a C_1-6alkyl group. Examples of C_1-6 alkoxy groups include, but are not limited to, -OMe (methoxy), -OEt (ethoxy), -O(nPr) (n-propoxy), -O(iPr) (isopropoxy), -O(nBu) (n-butoxy), -O(sHu) (sec-butoxy), -O(iBu) (isobutoxy), and -O(tBu) (tert-butoxy).

0078The term "halogen" as used herein includes fluorine, chlorine, bromine and iodine.

0079The nucleotide molecules of the present invention are suitable for use in many different methods where the detection of nucleotides is required.

0080DNA sequencing methods, such as those outlined in <patcit id="pcit0011" dnum="US5302509A"><text>U.S. Pat. No. 5,302,509</text></patcit> can be carried out using the nucleotide.

0081Preferably the blocked and labelled modified nucleotide constructs of the nucleotide bases A, T, C and G are recognised as substrates by the same polymerase enzyme. In the methods described herein, each of the nucleotides can be brought into contact with the target sequentially, with removal of non-incorporated nucleotides prior to addition of the next nucleotide, where detection and removal of the label and the blocking group, if present is carried out either after addition of each nucleotide, or after addition of all four nucleotides.

0082In the methods, all of the nucleotides can be brought into contact with the target simultaneously, i.e., a composition comprising all of the different nucleotides is brought into contact with the target, and non-incorporated nucleotides are removed prior to detection and subsequent to removal of the label and the blocking group, if present.

0083The four nucleotides, one of which will be complementary to the first unpaired base in the target polynucleotide, may be brought into contact with the target sequentially, optionally with removal of non-incorporated nucleotides prior to addition of the next nucleotide. Determination of the success of the incorporation may be carried out either after provision of each nucleotide, or after the addition of all of the nucleotides added. If it is determined after addition of fewer than four nucleotides that one has been incorporated, it is not necessary to provide further nucleotides in order to detect the nucleotides complementary to the incorporated nucleotide.

0084Alternatively, all of the nucleotides can be brought into contact with the target simultaneously, <i>i.e</i>., a composition comprising all of the different nucleotide (i.e. A, T, C and G or A, U, C and G) is brought into contact with the target, and non-incorporated nucleotides removed prior to detection and removal of the label(s). The'methods involving sequential addition of nucleotides may comprise a first substep and optionally one or more subsequent substeps. In the first substep a composition comprising one, two or three of the four possible nucleotides is provided, i.e. brought into contact with, the target. Thereafter any unincorporated nucleotides may be removed and a detecting step may be conducted to determine whether one of the nucleotides has been incorporated. If one has been incorporated, the cleavage of the linker may be effected and, if necessary, a terminal amide functionality introduced thereafter to the pendant arm. In this way the identity of a nucleotide in the target polynucleotide may be determined. The nascent polynucleotide may then be extended to determine the identity of the next unpaired nucleotide in the target oligonucleotide.

0085If the first substep above does not lead to incorporation of a nucleotide, or if this is not known, since the presence of incorporated nucleotides is not sought immediately after the first substep, one or more subsequent substeps may be conducted in which some or all of those nucleotides not provided in the first substep are provided either, as appropriate, simultaneously or subsequently. Thereafter any unincorporated nucleotides may be removed and a detecting step conducted to determine whether one of the classes of nucleotide has been incorporated. If one has been incorporated, cleavage of the linker may be effected, and if necessary as an additional step or steps, terminal amide functionality introduced to the pendant arm. In this way the identity of a nucleotide in the target polynucleotide may be determined. The nascent polynucleotide may then be extended to determine the identity of the next unpaired nucleotide in the target oligonucleotide. If necessary, a third and optionally a fourth substep may be effected in a similar manner to the second substep. Obviously, once four substeps have been effected, all four possible nucleotides will have been provided and one will have been incorporated.

0086It is desirable to determine whether a type or class of nucleotide has been incorporated after any particular combination comprising one, two or three nucleotides has been provided. In this way the unnecessary cost and time expended in providing the other nucleotide(s) is obviated. This is not a required feature of the invention, however.

0087It is also desirable, where the method for sequencing comprises one or more substeps, to remove any unincorporated nucleotides before further nucleotide are provided. Again, this is not a required feature of the invention. Obviously, it is necessary that at least some and preferably as many as practicable of the unincorporated nucleotides are removed prior to the detection of the incorporated nucleotide.

0088A method for determining the sequence of a target polynucleotide can be carried out by contacting the target polynucleotide separately with the different nucleotides to form the complement to that of the target polynucleotide, and detecting the incorporation of the nucleotides. Such a method makes use of polymerisation, whereby a polymerase enzyme extends the complementary strand by incorporating the correct nucleotide complementary to that on the target. The polymerisation reaction also requires a specific primer to initiate polymerisation.

0089For each cycle, the incorporation of the modified nucleotide is carried out by the polymerase enzyme, and the incorporation event is then determined. Many different polymerase enzymes exist, and it will be evident to the person of ordinary skill which is most appropriate to use. Preferred enzymes include DNA polymerase I, the Klenow fragment, DNA polymerase III, T4 or T7 DNA polymerase, Taq polymerase or Vent polymerase. Polymerases engineered to have specific properties can also be used. Preferably, the molecule is incorporated by a polymerase and particularly from <i>Thermococcus sp.,</i> such as 9°N. Even more preferably, the polymerase is a mutant 9°N A485L and even more preferably is a double mutant Y409V and A485L. An example of one such preferred enzyme is <i>Thermococcus</i> sp. 9°N exo -Y409V A485L available from New England Biolabs. Examples of such appropriate polymerases are disclosed in <nplcit id="ncit0020" npl-type="s"><text>Proc. Natl. Acad. Sci. USA, 1996(93), pp 5281-5285</text></nplcit>, <nplcit id="ncit0021" npl-type="s"><text>Nucleic Acids Research, 1999(27), pp 2454-2553</text></nplcit> and <nplcit id="ncit0022" npl-type="s"><text>Acids Research, 2002(30), pp 605-613</text></nplcit>.

0090Those skilled in the art will be aware of the utility of dideoxynucleoside triphosphates in socalled Sanger sequencing methods, and related protocols (Sanger-type), which rely upon randomised chain-termination at a particular type of nucleotide. An example of a Sanger-type sequencing protocol is the BASS method described by Metzker <i>(infra).</i> Other Sanger-type sequencing methods will be known to those skilled in the art.

0091Sanger and Sanger-type methods generally operate by the conducting of an experiment in which eight types of nucleotides are provided, four of which contain a 3'OH group; and four of which omit the OH group and which are labeled differently from each other. The nucleotides used which omit the 3'OH group - dideoxy nucleotides - are conventially abbreviated to ddNTPs. As is known by the skilled person, since the ddNTPs are labeled differently, by determining the positions of the terminal nucleotides incorporated, and combining this information, the sequence of the target oligonucleotide may be determined.

0092It will be recognized that the nucleotides of the present invention in which the 3'OH group is either absent or blocked may be of utility in Sanger methods and related protocols since the same effect achieved by using ddNTPs may be achieved by using 3'-OH blocking groups: both prevent incorporation of subsequent nucleotides.

0093The use of the nucleotides according to the present invention in Sanger and Sanger-type sequencing methods form a still further aspect of this invention. Viewed from this aspect, the invention provides the use of such nucleotides in a Sanger or a Sanger-type sequencing method.

0094The sequencing methods are preferably carried out with the target polynucleotide arrayed on a solid support. Multiple target polynucleotides can be immobilised on the solid support through linker molecules, or can be attached to particles, <i>e.g</i>., microspheres, which can also be attached to a solid support material.

0095The polynucleotides can be attached to the solid support by a number of means, including the use of biotin-avidin interactions. Methods for immobilizing polynucleotides on a solid support are well known in the art, and include lithographic techniques and "spotting" individual polynucleotides in defined positions on a solid support. Suitable solid supports are known in the art, and include glass slides and beads, ceramic and silicon surfaces and plastic materials. The support is usually a flat surface although microscopic beads (microspheres) can also be used and can in turn be attached to another solid support by known means. The microspheres can be of any suitable size, typically in the range of from 10 nm to 100 nm in diameter. In a preferred embodiment, the polynucleotides are attached directly onto a planar surface, preferably a planar glass surface. Attachment will preferably be by means of a covalent linkage. Preferably, the arrays that are used are single molecule arrays that comprise polynucleotides in distinct optically resolvable areas, <i>e.g</i>., as disclosed in International App. No. <patcit id="pcit0012" dnum="WO0006770A"><text>WO 00/06770</text></patcit>.

0096The sequencing method can be carried out on both single polynucleotide molecule and multi-polynucleotide molecule arrays, <i>i.e</i>., arrays of distinct individual polynucleotide molecules and arrays of distinct regions comprising multiple copies of one individual polynucleotide molecule. Single molecule arrays allow each individual polynucleotide to be resolved separately. The use of single molecule arrays is preferred. Sequencing single molecule arrays non-destructively allows a spatially addressable array to be formed.

0097The method makes use of the polymerisation reaction to generate the complementary sequence of the target. Conditions compatible with polymerization reactions will be apparent to the skilled person.

0098To carry out the polymerase reaction it will usually be necessary to first anneal a primer sequence to the target polynucleotide, the primer sequence being recognised by the polymerase enzyme and acting as an initiation site for the subsequent extension of the complementary strand. The primer sequence may be added as a separate component with respect to the target polynucleotide. Alternatively, the primer and the target polynucleotide may each be part of one single stranded molecule, with the primer portion forming an intramolecular duplex with a part of the target, <i>i.e</i>., a hairpin loop structure. This structure may be immobilised to the solid support at any point on the molecule. Other conditions necessary for carrying out the polymerase reaction, including temperature, pH, buffer compositions etc., will be apparent to those skilled in the art.

0099The modified nucleotides of the invention are then brought into contact with the target polynucleotide, to allow polymerisation to occur. The nucleotides may be added sequentially, <i>i.e</i>., separate addition of each nucleotide type (A, T, G or C), or added together. If they are added together, it is preferable for each nucleotide type to be labelled with a different label.

0100This polymerisation step is allowed to proceed for a time sufficient to allow incorporation of a nucleotide.

0101Nucleotides that are not incorporated are then removed, for example, by subjecting the array to a washing step, and detection of the incorporated labels may then be carried out.

0102Detection may be by conventional means, for example if the label is a fluorescent moiety, detection of an incorporated base may be carried out by using a confocal scanning microscope to scan the surface of the array with a laser, to image a fluorophore bound directly to the incorporated base. Alternatively, a sensitive 2-D detector, such as a charge-coupled detector (CCD), can be used to visualise the individual signals generated. However, other techniques such as scanning near-field optical microscopy (SNOM) are available and may be used when imaging dense arrays. For example, using SNOM, individual polynucleotides may be distinguished when separated by a distance of less than 100 nm, <i>e.g</i>., 10 nm to 10 µm. For a description of scanning near-field optical microscopy, see <nplcit id="ncit0023" npl-type="s"><text>Moyer et al., Laser Focus World 29:10, 1993</text></nplcit>. Suitable apparatus used for imaging polynucleotide arrays are known and the technical set-up will be apparent to the skilled person.

0103After detection, the label may be removed using suitable conditions that cleave the linker.

0104The use of the modified nucleotides is not limited to DNA sequencing techniques, and other techniques, including polynucleotide synthesis, DNA hybridisation assays and single nucleotide polymorphism studies, may also be carried out using nucleotides of the invention. Any technique that involves the interaction between a nucleotide and an enzyme may make use of the molecules of the invention. For example, the molecule may be used as a substrate for a reverse transcriptase or terminal transferase enzyme.

0105Suitable nucleotide structures are described in the following non-limiting Examples and are shown in the accompanying drawings.

3-(2,2-Diethoxy-ethoxy)-benzoic acid ethyl ester

0106<chemistry id="chem0025" num="0025"><img file="EP1560838B1_D0025.tif" /></chemistry> 2-Bromoacetaldehyde diethyl acetal (3 ml, 20 mmol), ethyl-3-hydroxy-benzoate (1.66g, 10mmol), potassium carbonate (2.76 g, 20 mmol) and sodium iodide (0.298 g, 2 mmol) were heated at 120 °C in dimethyl formamide (DMF) (15 ml) for 17hrs. Another batch of 2-bromoacetaldehyde diethyl acetal (3 ml, 20 mmol) was added and the reaction mixture was heated at 120 °C for another 24hrs. The reaction was cooled to room temperature and all the solvents were evaporated under reduced pressure. The residues were partitioned between dichloromethane (DCM) (200 ml) and water (200 ml). The DCM layer was separated and the aqueous layer was back-extracted with DCM (2 x 100 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The residue was purified by a column chromatography (4 x 20 cm). The product was eluted with 100% DCM and the title compound was obtained as a colourless oil (2.21 g, 78.3%). ¹HNMR [CDCl₃] : 7.58 (1H, Ar-H, d <i>J</i> 7.7), 7.51 (1H, Ar-H, dd, <i>J</i> 2.4 and 1.5), 7.27 (1H, Ar-H, t, <i>J</i> 8.0), 7.05 (1H, Ar-H, dd, <i>J</i> 7.9 and 2.3), 4.79 (1H, CH, t, <i>J</i> 5.2), 4.20 (2H, OCH₂, q, <i>J</i> 7.2), 3.99 (2H, ArOCH₂, d, <i>J</i> 5.2), 3.75-3.65 (2H, OCH₂, m), 3.63-3.53 (2H, OCH₂, m), 1.33 (3H, CH₃, t, <i>J</i> 7.2) and 1.19 (6H, CH₃, t, <i>J</i> 7.1) .

3-(2-Azido-2-ethoxy-ethoxy)-benzoic acid ethyl ester

0107<chemistry id="chem0026" num="0026"><img file="EP1560838B1_D0026.tif" /></chemistry> To a mixture of 3-(2,2-diethoxy-ethoxy)-benzoic acid ethyl ester (1.128 g, 4 mmol) and azidotrimethylsilane (0.584 ml, 4.4 mmol) was added SnCl₄ (40 µl) at room temperature. After 1 hr, the precipitates were filtered off and the filtrate was evaporated under reduced pressure. The residue was dissolved in methanol, after 10 minutes, solvent was removed under reduced pressure. The residue was purified by a column chromatography (3 x 20 cm). The product was eluted with 10% petroleum ether (60-80 °C) in DCM. The title compound was obtained as a colourless oil (0.909 g, 81.5%). ¹HNMR [CDCl₃] : 7.51 (1H, Ar-H, d <i>J</i> 7.7), 7.41 (1H, Ar-H, dd, <i>J</i> 2.6 and 1.5), 7.19 (1H, Ar-H, t, <i>J</i> 7.9), 6.96 (1H, Ar-H, ddd, <i>J</i> 8.2, 2.7 and 0.9), 4.63 (1H, CHN₃, t, <i>J</i> 5.1), 4.20 (2H, OCH₂, q, <i>J</i> 7.2), 4.05-3.90 (2H, ArOCH₂, m), 3.80-3.78 (1H, OCH₂, H_a, m), 3.55-3.47 (1H, OCH₂, H_b, m), 1.22(3H, CH₃, t, <i>J</i> 7. 1) and 1.13 (3H, CH₃, t, <i>J</i> 7.1).

3-(2-Azido-2-ethoxy-ethoxy)-benzoic acid

0108<chemistry id="chem0027" num="0027"><img file="EP1560838B1_D0027.tif" /></chemistry> 3-(2,2-Diethoxy-ethoxy)-benzoic acid ethyl ester (0.279 g, 1 mmol) was stirred with 4 M aqueous sodium hydroxide (2.5 ml, 10 mmol) and ethanol (2.5 ml) at room temperature. After 4 hrs, all the solvents were removed under reduced pressure and the residue was dissolved in 50 ml water. The solution was acidified with 1 N HCl to pH 2 and then extracted with DCM (3 x 50 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The title compound was obtained as a colourless solid (0.247 g, 98.4%). ¹HNMR [CDCl₃] : 7.68 (1H, Ar-H, d <i>J</i> 7.7), 7.58 (1H, Ar-H, dd, <i>J</i> 2.5 and 1.5), 7.34 (1H, Ar-H, t, <i>J</i> 8.0), 7.13 (1H, Ar-H, dd, <i>J</i> 7.4 and 2.7), 4.74 (1H, CHN₃, t, <i>J</i> 5.0), 4 .20-4 .02 (2H, ArOCH₂, m), 3.95-3.80 (1H, OCH₂, H_a, m), 3.70-3.55 (1H, OCH₂, H_b, m) and 1.24 (3H, CH₃, t, <i>J</i> 7.1).

Triethyl ammonium salt of phosphoric acid moo-[5-(5-{3-[3-(2-azido-2-ethoxy-ethoxy)-benzoylamino-prop-1-ynyl}-2,4-dioxo-3,4-dihydro-2<i>H</i>-pyrimidin-1-yl)-3-hydroxy-tetrahydro-furan-2-yl] ester

0109<chemistry id="chem0028" num="0028"><img file="EP1560838B1_D0028.tif" /></chemistry> 3-(2,2-Diethoxy-ethoxy).-benzoic acid (3.77 mg, 15, µmol) was stirred with <i>N,N</i>'-disuccinimidyl carbonate (3.84 mg, 15 µmol) and 4-dimethylaminopyridine (DMAP) (1.83 mg, 15 µmol) in dry DMF (1 ml) at room temperature. After 15 minutes, all the reaction mixture was added to a solution of the triethyl ammonium salt of phosphoric acid mono-{5-[5-(3-amino-prop-1-ynyl)-2,4-dioxo-3,4-dihydro-2<i>H</i>-pyrimidin-1-yl]-3-hydroxy-tetrahydro-furan-2-yl} ester (5 µmol) in 0.1 M carbonate buffer (0.1 M NaHCO₃/0.1 M Na₂CO₃, v/v, 1:1) (0.5 ml). After 5 hrs at room temperature, the reaction was diluted with 0.05 M triethylammonium bicarbonate (TEAB) buffer (TEAB, pH 7.5) (10 ml). The resulting solution was applied onto a short column of DEAE A-25 (1 x 5 cm). The column was initially eluted with 0.1 M TEAB buffer (50 ml) and then 0.7 M buffer (50 ml). The 0.7 M TEAB eluents were collected and evaporated under reduced pressure. The residue was co-evaporated with MeOH (2 x 10 ml) and then purified by preparative HPLC [HPLC gradient: A, 100% 0.1 M TEAB; B 100% MeCN; 0-2 min, 5% B (flow 2-10 ml/min); 2-19 min, 5-40% B (flow 10 ml/min); 19-21 min, 40-95% B (flow 10 ml/min); 21-24 min, 95% B (flow 10 ml/min); 24-26 min, 95-5% B (flow 10 ml/min); 26-30 min, 5% B (flow 10-2 ml/min)]. The product with retention time of 19.6 min was collected and evaporated under reduced pressure and the residue was co-evaporated with methanol (3 x 5 ml) to give the title compound as triethyl ammonium salt (3.67 mg, yield 80%). ¹HNMR in D₂O indicated approximately 3.2 triethylammonium count ions. ¹HNMR [D₂O] : 7.89 (1H, H-6, s), 7.44-7.31 (3H, Ar-H, m), 7.28 (1H, Ar-H, m), 7.12 (1H, Ar-H, dt, <i>J</i> 7.1 and 2.3), 6.17 (1H, H-1', t, <i>J</i> 6.9), 4.96 (1H, CHN₃, t, <i>J</i> 4.4), 4.43-4.35 (1H, H-3', m), 4.25 (2H, CHN, s), 4.18-4.06 (2H, H-5', m), 4.05-3.90 (1H, H-4', m), 3.89-3.50 (4H, ArOCH₂, OCH₂, m), 3.03 (19H, CH₂N, q, <i>J</i> 7.3), 2.25-2.15 (2H, CH₂, m), 1.13 (32H, CH₃, t, <i>J</i> 7.3) . ³¹P [D₂O] : 5.17 (s). MS-ES (-), m/z 593 [M-1].

2-(1-Azido-ethoxy)-ethanol

0110<chemistry id="chem0029" num="0029"><img file="EP1560838B1_D0029.tif" /></chemistry> To a mixture of 2-methyl dioxolane (0.897 ml, 10 mmol) and azidotrimethylsilane (1.6 ml, 12 mmol) was added SnCl₄ (40 µl) at -78 °C. After addition, the cooling bath was removed and the reaction was warmed up to room temperature. After 1 hr, the reaction was worked up by partitioning between DCM (50 ml) and saturated aqueous NaHCO₃ (50 ml). The aqueous layer was further extracted with DCM (20 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The residue was dissolved in 10% aqueous methanol. After 2 hr at room temperature, all the solvents were removed under reduced pressure. The title compound was obtained as a colourless oil (224 mg, 17.1%). ¹HNMR [CDCl₃] : 4.46 (1H, CHN₃, q, <i>J</i> 5.7), 3.77-3.68 (1H, OCH₂, H_a, m), 3.63 (2H, OCH₂, t, <i>J</i> 4.3), 3.48-3.40 (1H, OCH₂, H_b, m) and 1.34 (3H, CH₃, d, <i>J</i> 5.7) .

[2-(1-Azido-ethoxy)-ethoxy]-acetic acid ethyl ester

0111<chemistry id="chem0030" num="0030"><img file="EP1560838B1_D0030.tif" /></chemistry> To a solution of 2-(1-azido-ethoxy)-ethanol (0.15 g, 1.14 mmol) in dry tetrahydrofuran (THF) (5 ml) was added NaH (60% dispersion, 0.08 g, 2 mmol) at 0 °C. After 15 minutes, ethyl-2-bromoacetate (0.222 ml, 2 mmol) was added. The reaction was maintained at this temperature for 15 minutes and then warmed up to room temperature. The reaction was quenched by addition of saturated aqueous NaHCO₃ (5 ml) after 1 hr. After a further period of 5 min, the mixture was partitioned between DCM (50 ml) and saturated aqueous NaHCO₃ (50 ml). The aqueous layer was further extracted with DCM (50 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The title compound was obtained as an oil (0.162 g, 65.5%) . ¹HNMR [CDCl₃] : 4.51 (1H, CHN₃, q, <i>J</i> 5.6), 4.10 (2H, OCH₂, q, <i>J</i> 7.1), 4.03 (2H, OCH₂C(O), s), 3.85-3.77 (1H, OCH₂, H_a, m), 3.67-3.57 (3H, OCH₂, H_b, OCH₂, m), 1.37 (3H, CH₃, d, <i>J</i> 5.7) and 1.17 (3H, CH₃, t, <i>J</i> 7.1) .

[2-(1-Azido-ethoxy)-ethoxy]-acetic acid

0112<chemistry id="chem0031" num="0031"><img file="EP1560838B1_D0031.tif" /></chemistry> [2-(1-Azido-ethoxy)-ethoxy]-acetic acid ethyl ester (0.10 g, 0.46 mmol) was stirred with 4 M aqueous sodium hydroxide (1.15 ml, 4.6 mmol) and ethanol (1.15 ml) at room temperature. After 4 hrs, all the solvents were removed under reduced pressure and the residue was dissolved in 5 ml water. The solution was adjusted to pH 5 with 1 N KH₂PO₄ and then extracted with DCM (2 x 15 ml). The aqueous layer was then acidified with 1 N HCl to pH 2 and then extracted with DCM (3 x 25 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The title compound was obtained as a colourless solid (42 mg, 48.3%). ¹HNMR [CDCl₃] : 4.55 (1H, CHN₃, q, <i>J</i> 5.7), 4.15 (2H, OCH₂C(O), s), 3.92-3.84 (1H, OCH₂, H_a, m), 3.74-3.70 (2H, OCH₂, m), 3.68-3.57 (1H, OCH₂, H_b, m) and 1.44 (3H, CH₃, d, <i>J</i> 5.7).

Triethyl ammonium salt of phosphoric acid mono-{5-[5-(3-{2-[2-(1-azido-ethoxy)-ethoxy]-acetylamino}-prop-1-ynyl)-2,4-dioxo-3,4-dihydro-2<i>H</i>-pyrimidin-1-yl]-3-hydroxy-tetrahydro-furan-2-yl} ester

0113<chemistry id="chem0032" num="0032"><img file="EP1560838B1_D0032.tif" /></chemistry> [2-(1-Azido-ethoxy)-ethoxy]-acetic acid (5.7 mg, 30 µmol) was stirred with <i>N,N</i>'-disuccinimidyl carbonate (7.68 mg, 30 µmol) and DMAP (3.7 mg, 30 µmol) in dry DMF (2 ml) at room temperature. After 10 minutes, all the reaction mixture was added to a solution of the triethyl ammonium salt of phosphoric acid mono-{5-[5-(3-amino-prop-1-ynyl)-2,4-dioxo-3,4-dihydro-2<i>H-</i>pyrimidin-1-yl]-3-hydroxy-tetrahydro-furan-2-yl} ester (10 µmol) in 0.1 M TEAB (0.5 ml). After 5 hrs at room temperature, the reaction was diluted with 0.05 M triethylammonium bicarbonate buffer (TEAB, pH 7.5) (10 ml). The resulting solution was applied onto a short column of DEAE A-25 (1 x 10 cm). The column was initially eluted with 0.1 M TEAB buffer (50 ml) and then 0.5 M buffer (50 ml). The 0.5 M TEAB eluents were collected and evaporated under reduced pressure. The residue was co-evaporated with MeOH (2 x 10 ml) and then purified by preparative HPLC [HPLC gradient: A, 100% 0.1 M TEAB; B 100% MeCN; 0-2 min, 5% B (flow 2-10 ml/min); 2-15 min, 5-15% B (flow 10 ml/min); 15-28 min, 15-22% B (flow 10 ml/min); 28-30 min, 22-95% B (flow 10 ml/min); 30-34 min, 95% B (flow 10 ml/min); 34-36 min, 95-5% B (flow 10 ml/min); 36-40 min, 5% B (flow 10-2 ml/min)]. The product with retention time of 21.3 min was collected and evaporated under reduced pressure and the residue was co-evaporated with methanol (3 x 5 ml) to give the title compound as triethyl ammonium salt (9.3 mmol, quantification at λ₂₈₈ in 0.1 M TEAB buffer, 93%). ¹HNMR in D₂O indicated approximately five triethylammonium count ions. ¹HNMR [D₂O]: 7.85 (1H, H-6, s), 6.15 (1H, H-1', t, <i>J</i> 6.8), 4.75 (1H, CHN₃, q, <i>J</i> 5.7), 4.38 (1H, H-3', m), 4.09 (2H, OCH₂C(O), s), 3.99 (2H, CHN, s), 3.95 (1H, H-4', m), 3.86-3.60 (6H, OCH₂CH₂O, H-5', m), 3.03 (30H, CH₂N, q, <i>J</i> 7.2), 2 .22-2. 12 (2H, CH₂, m), 1.31 (3H, CH₃, d, <i>J</i> 5.7) and 1.11 (45H, CH₃, t, <i>J</i> 7.2) . ³¹P [D₂O]: 5.14 (s). MS-ES(-), m/z, 531 [M-1].

3-([1,3]Dioxolan-2-ylmethoxy)-benzoic acid ethyl ester

0114<chemistry id="chem0033" num="0033"><img file="EP1560838B1_D0033.tif" /></chemistry> 2-Bromomethyl-1, 3-dioxolane (8.3 ml, 80 mmol), ethyl-3-hydroxy-benzoate (3.32g, 20mmol), potassium carbonate (5.53 g, 40 mmol) and sodium iodide (1.2 g, 8 mmol) were heated at 120 °C in DMF (8 ml) for 17hrs. The reaction was cooled to room temperature and all the solvents were evaporated under reduced pressure. The residues were partitioned between DCM (250 ml) and water (250 ml). The DCM layer was separated and the aqueous layer was back-extracted with DCM (2 x 100 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The residue was purified by column chromatography (4 x 25 cm). The product was eluted with 20% petroleum ether (60-80 °C) in DCM and the title compound was obtained as a slightly brown oil (4.63g, 91.8%). APCI-MS, m/z 252.95 (M+1). ¹HNMR [CDCl₃] : 1.39 (3H, CH₃, t, <i>J</i> 7.2), 3.96-4.09 (6H, OCH₂CH₂O ArOCH₂, m), 4.36 (2H, OCH₂, q, <i>J</i> 7.2), 5.31 (1H, CH, t, <i>J</i> 4.0), 7.14 (1H, Ar-H, ddd, <i>J</i> 1.6, 2.6 and 8.2), 7.34 (1H, Ar-H, t, <i>J</i> 7.9), 7.59 (1H, Ar-H, dd, <i>J</i> 1.5 and 2.5) and 7.67 (1H, Ar-H, dt, <i>J</i> 1.4 and 7.6).

3-[2-Azido-2-(2-hydroxy-ethoxy)-ethoxy]-benzoic acid ethyl ester

0115<chemistry id="chem0034" num="0034"><img file="EP1560838B1_D0034.tif" /></chemistry> To a mixture of 3-([1, 3]-dioxolan-2-ylmethoxy)-benzoic acid ethyl ester (2.02 g, 8 mmol) and azidotrimethylsilane (1.17 ml, 8.8 mmol) was added SnCl₄ (60 µl) at room temperature under nitrogen. After 2 hr, 2% aqueous methanol (10 ml) was added to the reaction mixture and the reaction was stirred at room temperature for 30 minutes. All the solvents were evaporated under reduced pressure. The residue was co-evaporated with ethanol (2 x 10 ml). The residue was purified by a column chromatography (3 x 20 cm). The product was eluted with 0 to 1% methanol in DCM. The title compound was obtained as a colourless oil (2.01 g, 85.1%). APCI-MS, m/z 267.90 (M-N₂+1). ¹HNMR [CDCl₃]: 1.38 (3H, CH₃, t, <i>J</i> 7.1), 3.73-3.86 (3H, OCH₂, H_a, OCH₂, m), 3.99-4.05 (1H, OCH₂, H_b, m), 4.17 (1H, Ar-OCH₂ , H_a, dd, <i>J</i> 4.9 and 10.1), 4.23 (1H, ArOCH₂, H_b, dd, <i>J</i> 5.2 and 10.1), 4.38 (2H, OCH₂, q, <i>J</i> 7.1), 4.89 (1H, CH-N₃, t, <i>J</i> 5.1), 7.13 (1H, Ar-H, dd, <i>J</i> 2.1 and 8.4), 7.36 (1H, Ar-H, t, <i>J</i> 7.9), 7.60 (1H, Ar-H, dd, <i>J</i> 1.0 and 2.5) and 7.70 (1H, Ar-H, d, <i>J</i> 7.8).

3-[2-Azido-2-(2-hydroxy-ethoxy)-ethoxy]- benzoic acid

0116<chemistry id="chem0035" num="0035"><img file="EP1560838B1_D0035.tif" /></chemistry> 3-[2-Azido-2-(2-hydroxy-ethoxy)-ethoxy]-benzoic acid ethyl ester (1.34 g, 4.55 mmol) was stirred with 4 M aqueous sodium hydroxide (11.4 ml, 45.5 mmol) and ethanol (11.4 ml) at room temperature. After 3 hrs, all the solvents were removed under reduced pressure and the residue was dissolved in 50 ml water. The solution was acidified with 1 N HCl to pH 2 and then extracted with DCM (3 x 50 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The title compound was obtained as a colourless solid (1.2 g, 98.7%). ES-MS, m/z 265.85 (M-1). ¹HNMR [CDCl₃] : 3.75-3.90 (3H, OCH₂, H_a, OCH₂, m), 4. 00-4.08 (1H, OCH₂, H_b, m), 4.17 (1H, Ar-OCH₂, H_a, dd, <i>J</i> 4.8 and 10.1), 4.24 (1H, ArOCH₂, H_b, dd, <i>J</i> 5.1 and 10.1), 4.90 (1H, CH-N₃, t, <i>J</i> 5.1), 7.19 (1H, Ar-H, dd, <i>J</i> 2.5 and 8.2), 7.40 (1H, Ar-H, t, <i>J</i> 8.0), 7.60 (1H, Ar-H, s) and 7.70 (1H, Ar-H, d, <i>J</i> 7.9).

3-[2-Azido-2-(2-ethoxycarbonylmethoxy-ethoxy)-ethoxy]-benzoic acid

0117<chemistry id="chem0036" num="0036"><img file="EP1560838B1_D0036.tif" /></chemistry> To a solution of 3-[2-azido-2-(2-hydroxy-ethoxy)-ethoxy]-benzoic acid (0.535 g, 2 mmol) in dry THF (6 ml) was added NaH (60% dispersion, 0.246 g, 6 mmol) at 0 °C. After 10 minutes, ethyl-2-bromoacetate (0.488 ml, 4.4 mmol) was added. The reaction was then warmed up to room temperature and stirred for 4 hours. The reaction was quenched by pouring it into ice-cold water (50 ml). The mixture was extracted with DCM (2 x 50 ml) and the DCM extracts were discarded. The aqueous layer was then acidified to pH 2 with 1 N HCl, and extracted with DCM (2 x 50 ml). These DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The residue was purified by column chromatography (1 x 20 cm). The title compound, eluted with 2% methanol in DCM, was obtained as an oil (0.223 g, 31.6%). ES-MS, m/z 351.95 (M-1). ¹HNMR [CDCl₃] : 1.29 (3H, CH₃, t, <i>J</i> 7.2), 3.81 (2H, OCH₂, t, <i>J</i> 4.4), 3.90 (1H, OCH₂, H_a, m), 4.04 (1H, OCH , H_b, m), 4.13-4.27 (6H, ArOCH₂ , OCH₂ and OCH₂C(O)), 4.95 (1H, CH-N₃, m), 7.19 (1H, Ar-H, dd, <i>J</i> 1. 7 and 8.3), 7.40 (1H, Ar-H, t, <i>J</i> 7.8), 7.63 (1H, Ar-H, s) and 7.76 (1H, Ar-H, d, <i>J</i> 7.6).

[2-(1-Azido-2-{3-[2-(2,2,2-trifluoroacetylamino)-ethylcarbamoyl]-phenoxy}-ethoxy)-ethoxy]-acetic acid ethyl ester

0118<chemistry id="chem0037" num="0037"><img file="EP1560838B1_D0037.tif" /></chemistry> 3-[2-Azido-2-(2-ethoxycarbonylmethoxy-ethoxy)-ethoxy]-benzoic acid (0.212 g, 0.6 mmol) was stirred with N,N'-disuccinimidyl carbonate (0.184 g, 0.72 mmol) and DMAP (0.088g, 0.72 mmol) in dry DMF (1 ml) at room temperature. After 10 minutes, trifluoroacetic acid salt of N-(2-amino-ethyl)-2,2,2-trifluoro-acetamide (0.194 g, 0.72 mmol) was added followed by diisopropylethylamine (DIPEA) (0.251 ml, 1.44 mmol). The reaction mixture was then stirred at room temperature for 17 hours. All the solvents were evaporated under reduced pressure and the residues were partitioned between DCM (50 ml) and aqueous NaH₂PO₄ (1 N, 50 ml). The aqueous layer was further extracted with DCM (2 x 25 ml). All the DCM extracts were combined, dried over MgSO₄ and evaporated under reduced pressure. The residue was purified by column chromatography (1 x 20 cm). The title compound, eluted with 1 % methanol in DCM, was obtained as an oil (0.255 g, 86.6%). ES-MS, m/z 490.10 (M-1) . ¹HNMR [CDCl₃]: 1.28 (3H, CH₃, t, <i>J</i> 7.1), 3.60 (2H, CH₂N, m), 3.69 (2H, CH₂N, m), 3.80 (2H, OCH₂, t, <i>J</i> 4.3), 3. 86 (1H, OCH₂, H_a, m), 4.04 (1H, OCH₂, H_b, m), 4.10-4.25 (6H, ArOCH₂, OCH₂ and OCH₂C(O), m), 4.92 (1H, CH-N₃, m), 6.85 (1H, NH, br), 7.09 (1H, Ar-H, m), 7.37 (3H, Ar-H, m), and 7.96 (1H, NH, br) .

(2-{2-[3-(2-Amino-ethylcarbamoyl)-phenoxy]-1-azido-ethoxy}-ethoxy)-acetic acid

0119<chemistry id="chem0038" num="0038"><img file="EP1560838B1_D0038.tif" /></chemistry> [2-(1-Azido-2-{3-[2-(2,2,2-trifluoroacetylamino)-ethoxycarbamoyl]-phenoxy}-ethoxy)-ethoxy]-acetic acid ethyl ester (0.196 g, 0.4 mmol) was stirred with 4 M aqueous sodium hydroxide (1 ml, 4 mmol) and ethanol (1 ml) at room temperature. After 2 hrs, all the solvents were removed under reduced pressure and the residue was dissolved in 15 ml water. The solution was extracted with DCM (2 x 15 ml). The DCM extracts were discarded and the aqueous layer was acidified with 1 N HCl to pH 2. Then the solution was extracted again with DCM (3 x 15 ml). The DCM extracts were discarded and the aqueous layer was neutralised with 1 N NaOH to pH 8 and then evaporated under reduced pressure to dryness. The white solids were triturated with DCM/MeOH (v/v; 1:1, 2 x 25 ml). All the solids were filtered off and the filtrates were combined and evaporated under reduced pressure to give a gum. The gum was added in 10% MeOH in DCM (15 ml) and the insoluble, white solids were filtered off. The filtrates were evaporated under reduced pressure to give the mono-sodium salt of the title compound as a colourless powder (0.135 g, 86.6 %). ES-MS, m/z 368.00 (M+1). ¹HNMR [D₂O]: 3.01 (2H, CH₂NH₂, t, <i>J</i> 6.0), 3.51 (2H, CH₂N, t, <i>J</i> 6.0), 3.62 (2H, OCH₂, m), 3.77 (1H, OCH₂, H_a, m), 3.80 (2H, CH₂C(O), s), 3.96 (1H, OCH₂, H_b, m), 4.19 (2H, ArOCH₂, d, <i>J</i> 4.3), 5.01 (1H, CH-N₃, t, <i>J</i> 4.5), 7.13 (1H, Ar-H, d, <i>J</i> 7.9) and 7.25-7.39 (3H, Ar-H, m) .

[2-(1-Azido-2-{3-[2-(6-Cy3-hexanoylamino)-ethylcarbamoyl]-phenoxy}-ethoxy)-ethoxy]-acetic acid

0120<chemistry id="chem0039" num="0039"><img file="EP1560838B1_D0039.tif" /></chemistry> The commercial Cy3 mono <b><i>N</i></b>-hydroxysuccinimide ester (5 mg, 6.53 µmol) and (2-{2-[3-(2-amino-ethylcarbamoyl)-phenoxy]-1-azido-ethoxy}-ethoxy)-acetic acid (7.2 mg, 19.6 µmol) were stirred together in dry DMF. DIPEA (6.82 µl, 39.2 µmol) was added. After 2 hr stirring at room temperature, all the solvent was evaporated under reduced pressure. The residue was purified by preparative HPLC [HPLC gradient: A, 100% 0.1 M TEAB; B 100% MeCN; 0-2 min, 5% B (flow 2-10 ml/min); 2-19 min, 5-45% B (flow 10 ml/min); 19-21 min, 45-95% B (flow 10 ml/min); 21-24 min, 95% B (flow 10 ml/min); 24-26 min, 95-5% B (flow 10 ml/min); 26-30 min, 5% B (flow 10-2 ml/min)]. The title compound with retention time of 17.85 min was obtained as a pink solid (4.93 µmol, 75.5%, quantification at 550 nm in water). ES-MS, m/z 488. 95 [(M/2)-1]. ¹HNMR in D₂O indicated approximately 1.8 triethylammonium count ions. ¹HNMR [D₂O] : 1.15 (16.2H, CH₃ (Et₃N), t, <i>J</i>7.2), 1.21 (3H, CH₃, t, <i>J</i> 7.1), 1.47 (2H, CH₂, m), 1.55 (2H, CH₂, m), 1.58 (6H, 2 x CH₃, s), 1.61 (6H, 2 x CH₃, s), 2.10 (2H, CH₂C (O) , t, <i>J</i> 6.5), 3.06 (10.8 H, CH₂ (Et₃N) , q, <i>J</i> 7.2), 3.23 (2H, CH₂N, t, <i>J</i> 5.5), 3.32 (2H, CH₂N, t, <i>J</i> 5.8), 3.56 (2H, OCH₂, m), 3.67-3.78 (3H, OCH₂, H_a and CH₂N, m), 3.79 (2H, OCH₂C(O), s), 3.85-3.97 (3H, OCH₂, H_b and CH₂N, m), 3.98 (2H, ArOCH₂, d, <i>J</i> 4.4), 4.85 (1H, CH-N₃, t, <i>J</i> 4.3), 6.14 (1H, =CH, d, <i>J</i> 13.4), 6.19 (1H, =CH, d, <i>J</i> 13.4), 6.90 (1H, Ar-H, m), 7.10-7.19 (5H, Ar-H, m), 7.69 (2H, Ar-H, d, <i>J</i> 8.4), 7.73 (1H, Ar-H, s), 7.77 (1H, Ar-H, s) and 8.36 (1H, =CH, t, <i>J</i> 13.4).

5-[3-(-Cy3-azidolinkeracetylamino)-prop-1-ynyl]-3'-azidomethoxy-dUTP

0121<chemistry id="chem0040" num="0040"><img file="EP1560838B1_D0040.tif" /></chemistry> [2-(1-Azido-2-{3-[2-(6-Cy3-hexanoylamino) - ethylcarbamoyl]-phenoxy}-ethoxy)-ethoxy]-acetic acid (2 µmol) was stirred with <i>N,N</i>'-disuccinimidyl carbonate (0.563 mg, 2.2 µmol) and DMAP (0.269 mg, 2.2 µmol) in dry DMF (1 ml) at room temperature. After 10 minutes, all the reaction mixture was added to a solution of the tri-n-butyl ammonium salt of [5-(3-amino-prop-1-ynyl)]-3'-azidomethoxy-dUTP (6 µmol, prepared by evaporating an aqueous solution of [5-(3-amino-prop-1-ynyl)]-3'-azidomethoxy-dUTP with tri-n-butyl amine (72 µl, 300 µmol)). The reaction mixture was sonicated for 5 minutes, then stirred at room temperature for 3hrs. The reaction mixture was diluted with chilled 0.1 M TEAB (10 ml), the resulting solution was applied onto a short column of DEAE A-25 (1 x 10 cm). The column was initially eluted with 0.1 M TEAB buffer (50 ml) and then 1.0 M buffer (50 ml). The 1.0 M TEAB eluates were collected and evaporated under reduced pressure. The residue was co-evaporated with MeOH (2 x 10 ml) and then purified by semi-preparative HPLC [HPLC gradient: A, 100% 0.1 M TEAB; B 100% MeCN; 0-2 min, 5% B (flow 2-5 ml/min); 2-14 min, 5-20% B (flow 5 ml/min); 14-20 min, 20-23% B (flow 5 ml/min); 20-22 min, 23-95% B (flow 5 ml/min); 22-25 min, 95% B (flow 5 ml/min); 25-26 min, 95-5% B (flow 5 ml/min); 26-30 min, 5% B (flow 5-2 ml/min)]. The product with retention time of 19.2 min was collected and evaporated under reduced pressure and the residue was co-evaporated with methanol (3 x 5 ml) to give the title compound as triethyl ammonium salt (1.22 µmol, quantification at λ₅₅₀ in 0.1 M TEAB buffer, 61.1%). ¹HNMR in D₂O indicated approximately seven triethylammonium count ions. ¹HNMR [D₂O]: 1.14 (63H, CH₃ (Et₃N), t, <i>J</i> 7.3), 1.23 (3H, CH₃, t, <i>J</i> 7.1), 1.49 (2H, CH₂, m), 1.55 (2H, CH₂, m), 1.60 (6H, 2 x CH₃, s), 1.62 (6H, 2 x CH₃, s), 1.95-2.07 (1H, H_a-2' , m), 2.13 (2H, CH₂C(O), t, <i>J</i> 6.7), 2.17-2 .27 (1H, H_b-2', m), 3.05 (42 H, CH₂ (Et₃N), q, <i>J</i> 7.3), 3.27 (2H, CH₂N, t, <i>J</i> 5.7), 3.37 (2H, CH₂N, t, <i>J</i> 5.8), 3.55-3.79 (5H, m), 3.80-4.10 (11H, m), 4.15 (1H, H-4', m), 4.38 (1H, H-3', m), 4.80 (2H, OCH2-N₃, s), 4.88 (1H, CH-N₃, m), 5.95 (1H, H-1', q, <i>J</i> 7.6), 6.13 (1H, =CH, d, <i>J</i> 13.4), 6.20 (1H, =CH, d, <i>J</i> 13.4), 6.84 (1H, Ar-H, d, <i>J</i> 7.3), 7.05-7.24 (5H, Ar-H, m), 7.60-7.80 (5H, Ar-H and H-6, m) and 8.35 (1H, =CH, t, <i>J</i> 13.4). ³¹PNMR [D₂O] : -20.82 (^βP, m), -10.07 (^αP, d, J 16.2) and -4.90 (^γP, d, J 18.9).

2 cycles of fully functional nucleoside triphosphate (FFN)

Preparation of beads

0122Take 15 µL of Dynabeads^® M-280 streptavidin coated beads (Dynal Biotech), remove storage buffer and wash 3 times with 150 µL of TE buffer (Tris.HCl pH 8, 10 mM and EDTA, 1 mM). Resuspend in 37.5 µL of B &amp; W buffer(10 mM Tris-HCl pH 7.5, 1 mM EDTA and 2.0 M NaCl), add 10 µL of biotinylated ³²P labelled hairpin DNA and add 27.5 µL of water. Allow to stand at room temperature for 15 minutes. Remove buffer and wash beads 3 times with 100 µL of TE buffer.

Incorporation of the 1^st FFN

012375 µL reaction, Tris.HCl pH 8.8 50 mM, Tween-20, 0.01%, MgSO₄ 4 mM, MnCl₂, 0.2 mM, add 2 µM FFN and 100 nM polymerase (<i>Thermococcus sp.</i> 9°N exo ^-Y409V A485L supplied by New England Biolabs). This solution is then added to the beads and mixed thoroughly and incubated at 65°C taking 5µL samples at 3 and 10 minutes and stopping with 5 µL of gel loading buffer (xylene cyanol - bromophenol blue dye solution, Sigma-Aldrich). The reaction mixture is removed from the remaining beads and the beads washed 3 times with 100 µL of TE buffer.

Chase step

0124A sample was removed from the incorporation reaction and added to 1µM of dNTPs (0.25 µM each). This was stopped after 10 minutes by adding 5 µL of gel loading buffer.

Deblocking step

012550 µL of Tris-(2-carboxyethyl)phosphine,trisodium salt(TCEP) 0.1M is added to the beads and mixed thoroughly. The mixture was then incubated at 65°C for 15 minutes. The deblocking solution is removed and the beads washed 3 times with 100 µL TE buffer. The beads were then resuspended in 50 µL of TE and a 5 µL sample was removed and 5 µL of gel loading buffer)

Incorporation of the 2^nd FFN

012620 µL reaction, Tris.HCl pH 8.8 50 mM, Tween-20, 0.01%, MgSO₄ 4 mM, MnCl₂, 0.4 mM, add 2 µM FFN and 100 nM polymerase (<i>Thermococcus sp.</i> 9°N exo Y409V A485L supplied by New England Biolabs). This solution is then added to the beads and mixed thoroughly and incubate at 65°C taking 5µL samples at 3 and 10 minutes and stopping with 5 µL of gel loading buffer.

Chase step

0127A sample was removed from the incorporation reaction and added to 1µM of dNTPs (0.25 µM each). This was stopped after 10 minutes by adding 5 µL of gel loading buffer.

0128Before loading the samples onto a denaturing 12% acrylamide sequencing gel 0.5 µL of EDTA (0.5 M) was added to each sample and then heated to 95 °C for 10 minutes.