EP1546316A4

Mutant e. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same

Abstract

Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement.

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Term ended

Projected expiry passed 11 August 2023, 3.1 years ago.

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Citations
DocumentRelationOfficeCategoryCited duringRelevant claims
WO0136607A1CitesWorld Intellectual Property Organization (WIPO)XSearch report1-6,9,10
WO0190333A2CitesWorld Intellectual Property Organization (WIPO)XSearch report1-15,19
WO0248332A2CitesWorld Intellectual Property Organization (WIPO)XSearch report1,2
WO0190333ACitesWorld Intellectual Property Organization (WIPO)
WO0136607ACitesWorld Intellectual Property Organization (WIPO)
RODRIGUEZ E ET AL: "Cloning, sequencing, and expression of an Escherichia coli acid phosphatase/phytase gene (appA2) isolated from pig colon.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2 APR 1999, vol. 257, no. 1, 2 April 1999 (1999-04-02), pages 117 - 123, XP002129675, ISSN: 0006-291XNon-patentSearch report
OSTANIN K ET AL: "Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 15 NOV 1992, vol. 267, no. 32, 15 November 1992 (1992-11-15), pages 22830 - 22836, XP002129674, ISSN: 0021-9258Non-patentSearch report