EP1136822A3

Method for identifying cell specific target structures

Abstract

The present invention relates to a method for identifying cell-specific target structures, comprising the following steps:a) automated application of a reagent solution Y1 having at least one marker molecule to an object X1 having cells and / or cell membranes derived from a cell or tissue sample;b) acting on the reagent solution Y1 and automated detection of at least one marking pattern of the object X1 labeled with the reagent solution Y1;c) removing the reagent solution Y1 before or after the detection of the marking pattern and repeating the steps a) and b) with further reagent solutions Yn (n = 2, 3... N), each of the at least one marker molecule and / or at least one other Having marker molecule;d) combining the marking patterns respectively detected in step b) into a complex molecular combination pattern of the object X1;e) repeating steps a) to d) with at least one further object Xn (n = 2, 3... N) having other cells and / or other cell membranes derived from another cell or tissue sample;f) determining at least one difference between the combination pattern of the object X1 and the combination pattern of the object Xn;g) identifying at least one reagent solution Y1 or Yn whose labeling pattern causes the difference determined in step f);h) selection of molecules or molecular complexes which are bound by the at least one labeling molecule of the reagent solution Y1 or Yn identified in step g), from a homogenate of cells and / or cell membranes which are the cell or tissue sample of the one differing according to step f) Object Xn come from; andi) biochemical characterization of the selected according to step h) molecules or molecular complexes.

Term

Term ended

Projected expiry passed 15 March 2021, 5.5 years ago.

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Every citation, both waysCites: the store holds 5 of 6

Citations
DocumentRelationOfficeCategoryCited duringRelevant claims
EP0701130A2CitesEuropean Patent Office (EPO)ASearch report1-14
DE19709348A1CitesGermanyXSearch report1-14
US5437987ACitesUnited States of AmericaASearch report1-14
WO9318068A1CitesWorld Intellectual Property Organization (WIPO)ASearch report1-14
WO9534817A1CitesWorld Intellectual Property Organization (WIPO)ASearch report1-14