Method for identifying cell specific target structures
Abstract
The present invention relates to a method for identifying cell-specific target structures, comprising the following steps:a) automated application of a reagent solution Y1 having at least one marker molecule to an object X1 having cells and / or cell membranes derived from a cell or tissue sample;b) acting on the reagent solution Y1 and automated detection of at least one marking pattern of the object X1 labeled with the reagent solution Y1;c) removing the reagent solution Y1 before or after the detection of the marking pattern and repeating the steps a) and b) with further reagent solutions Yn (n = 2, 3... N), each of the at least one marker molecule and / or at least one other Having marker molecule;d) combining the marking patterns respectively detected in step b) into a complex molecular combination pattern of the object X1;e) repeating steps a) to d) with at least one further object Xn (n = 2, 3... N) having other cells and / or other cell membranes derived from another cell or tissue sample;f) determining at least one difference between the combination pattern of the object X1 and the combination pattern of the object Xn;g) identifying at least one reagent solution Y1 or Yn whose labeling pattern causes the difference determined in step f);h) selection of molecules or molecular complexes which are bound by the at least one labeling molecule of the reagent solution Y1 or Yn identified in step g), from a homogenate of cells and / or cell membranes which are the cell or tissue sample of the one differing according to step f) Object Xn come from; andi) biochemical characterization of the selected according to step h) molecules or molecular complexes.
Term
Term ended
Projected expiry passed 15 March 2021, 5.5 years ago.
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Every citation, both waysCites: the store holds 5 of 6
| Document | Relation | Office | Category | Cited during | Relevant claims |
|---|---|---|---|---|---|
| EP0701130A2 | Cites | European Patent Office (EPO) | A | Search report | 1-14 |
| DE19709348A1 | Cites | Germany | X | Search report | 1-14 |
| US5437987A | Cites | United States of America | A | Search report | 1-14 |
| WO9318068A1 | Cites | World Intellectual Property Organization (WIPO) | A | Search report | 1-14 |
| WO9534817A1 | Cites | World Intellectual Property Organization (WIPO) | A | Search report | 1-14 |
15 members in 8 offices
Priority claims5
| Document | Office | Kind | Date |
|---|---|---|---|
| 10014685 | Germany | A | |
| 10014685 | Germany | A | |
| 10014685 | Germany | – | |
| 10014685 | – | – | – |
| DE2000114685 | – | – | – |
Members15
| Document | Office | Kind | |
|---|---|---|---|
| EP1136822A2 | European Patent Office (EPO) | A2 | |
| DE10014685A1 | Germany | A1 | |
| JP2001309799A | Japan | A | |
| US2001039023A1 | United States of America | A1 | |
| CN1334463A | China | A | |
| EP1136822A3This record | European Patent Office (EPO) | A3 | |
| DE10014685B4 | Germany | B4 | |
| SG105488A1 | Singapore | A1 | |
| EP1136822B1 | European Patent Office (EPO) | B1 | |
| AT298426T | Austria | T | |
| ATE298426T1 | Austria | T1 | |
| DE50106565D1 | Germany | D1 | |
| US6924115B2 | United States of America | B2 | |
| CN1213300C | China | C | |
| ES2244509T3 | Spain | T3 |
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Numbers
- Publication
- 1136822
- Publication, DOCDB
- 1136822
- Publication, EPODOC
- EP1136822
- Application
- 1106571
- Application, DOCDB
- 01106571
- Application, EPODOC
- EP20010106571
Titles3
- German
- Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
- English
- Method for identifying cell specific target structures
- French
- Méthode pour l'identification des structures cibles spécifiques aux cellules
Classification
- CPC, 2
- G01N1/30
- G01N33/6803
- IPC, 4
- C12Q1 02
- C12R1 91
- G01N1 30
- G01N33 68
Designated states26
- Contracting states, 20
- Austria
- Belgium
- Switzerland
- Cyprus
- Germany
- Denmark
- Spain
- Finland
- France
- United Kingdom
- Greece
- Ireland
- Italy
- Liechtenstein
- Luxembourg
- Monaco
- Netherlands (Kingdom of the)
- Portugal
- Sweden
- Türkiye
- Extension states, 6
- Albania
- Lithuania
- Latvia
- North Macedonia
- Romania
- Slovenia