Monocyclic l-nucleosides, analogs and uses thereof

FIELD OF THE INVENTION

The present invention relates to the field of L-nucleosides.

BACKGROUND OF THE INVENTION

The last few decades have seen significant efforts expended in exploring possible uses of D-nucleoside analogs as antiviral agents. Some of this work has borne fruit, and a number of nucleoside analogs are currently being marketed as antiviral drugs, including the HIV reverse transcriptase inhibitors (AZT, ddI, ddC, d4T, and 3TC).

Nucleoside analogs have also been investigated for use as immune system modulators, (Bennet, P. A et al., <i>J. Med. Chem.,</i><u>36</u>, 635, <b>1993</b>), but again with less than completely satisfactory results. For example, guanosine analogs such as 8-bromo-, 8-mercapto-, 7-methyl-8-oxoguanosine (Goodman, M. G. <i>Immunopharmacology</i>, <u>21</u>, 51-68, <b>1991</b>) and 7-thia-8-oxoguanosine (Nagahara, K. <i>J. Med. Chem</i>., <u>33</u>, 407-415, <b>1990;</b> U.S, Pat. No. 5,041,426) have been studied over the years for their ability to activate the immune system. These guanosine derivatives show excellent antiviral and/or antitumor activity <i>in vivo</i>. But, these C₈-substituted guanosines were unable to activate T-cells (Sharma, B. S. et al., <i>Clin. Exp. Metastasis</i>, <u>9</u>, 429-439, <b>1991</b>). The same was found to be true with 6-arylpyrimidinones (Wierenga, W. <i>Ann. N. Y. Acad</i>. <i>Sci</i>., <u>685</u>, 296-300, <b>1993</b>). In other research, a series of 3-deazapurine nucleosides were synthesized and evaluated as immunomodulating agents. U.S. Patent No. 4,309,419 describes the use of 3-deazaadenosine as being an inhibitor of the immune system. The β-D-nucleoside, β-2'-deoxy-3-deazaguanosine (U.S. Pat. No. 4,950,647) displayed the most potent immunoenhancing potency on activated T-cell response. Antiinflamatory and immunosuppressant activity has also been disclosed for certain 2'-deoxynucleosides (EPO Application 0 038 569). However, these compounds undergo facile in vivo metabolic cleavage of their glycosyl bond, which effectively inactivates their biological potency. Adenosine derivatives disclosed in U.S. Pat. No. 4,148,888 are also catabolized in vivo by deaminase enzymes. In still other research, Levamisole, a thymomimetic immunostimulant (Hadden et al, <i>Immunol. Today</i>, <u>14</u>, 275-280, <b>1993),</b> appears to act on the T-cell lineage in a manner similar to thymic hormones. Tucaresol (Reitz et al, Nature, 377, 71-75,1995), another T-cell stimulant, is now undergoing clinical trials. More recently, 6-substituted purine linker amino acid (Zacharie et al, J. Med. Che., 40, 2883-2894, 1997) has been described as a promising immunostimulant which may be targeted for those disease states which require an increased CTL or Th1 type response.

One possible target of immunomodulation involves stimulation or suppression of Th1 ahd Th2 lymphokines. Type I (Th1) cells produce interleukin 2 (IL-2), tumor necrosis factor (TNFα) and interferon gamma (IFNγ) and they are responsible primarily for cell-mediated immunity such as delayed type hypersensitivity and antiviral immunity. Type 2 (Th2) cells produce interleukins, IL4, IL-5, IL-6, IL-9, IL-10 and IL-13 and are primarily involved in assisting humoral immune responses such as those seen in response to allergens, e.g. IgE and lgG4 antibody isotype switching (Mosmann, 1989, <i>Annu Rev Immunol</i>, 7:145-173). D-guanosine analogs have been shown to elicit various effects on lymphokines IL-1, IL-6, IFNα and TNFα (indirectly) <i>in vitro</i> (Goodman, 1988, <i>Int J Immunopharmacol</i>, <b>10</b>, 579-88) and <i>in vivo</i> (Smee et al., 1991, <i>Antiviral Res</i> 15: <i>229)</i>. However, the ability of the D-guanosine analogs such as 7- thio-8-oxoguanosine to modulate Type 1 or Type 2 cytokines directly in T cells was ineffective or has not been described.

Significantly, most of the small molecule research has focused on the synthesis and evaluation of D-nucleosides. This includes Ribavirin (Witkowski, J. T. et al., <i>J. Med. Chem.</i>, <u>15,</u> 1150, <b>1972</b>), AZT (De Clercq, E. <i>Adv. Drug Res.</i>, <u>17</u>, 1, <b>1988</b>), DDI (Yarchoan, R. et al., <i>Science (Washington, D. C.)</i>, <u>245</u>, 412, <b>1989</b>), DDC (Mitsuya, H. et aL, <i>Proc. Natl. Acad. Sci. U. S. A</i>., <u>83</u>, 1911, <b>1986</b>), d4T (Mansuri, M. M. et aL, <i>J. Med. Chem</i>., <u>32</u>, 461, <b>1989</b>) and 3TC (Doong, S. L. et al., <i>Proc. Natl</i>. <i>Acad. Sci. U.S.A</i>., <u>88</u>, 8495-8599, 1991). In this handful of therapeutic agents, only 3TC which contains an unnatural modified L-ribose moiety, the enantiomer of natural D-ribose.

After the approval of 3TC by the FDA, a number of nucleosides with the unnatural L-configuration were reported as having potent chemotherapeutic agents against immunodeficiency virus (HIV), hepatitis B virus (HBV), and certain forms of cancer. These include (-)-β-L-1-[2-(hydroxymethyl)-1,3-oxathiolan-4-yl]-5-fluorocytosine (FTC; Furman, P. A, et al, <i>Antimicrob. Agents Chemother.</i>, <u>36</u>, 2686-2692, 1992), (-)-β-L-2',3'-dideoxypentofuranosyl-5-flurocytosine (L-FddC; Gosselin, G., et al, <i>Antimicrob. Agents Chemother</i>., <u>38</u>, 1292-1297, <b>1994),</b> (-)-β-L-1-[2-(hydroxymethyl)-1,3-oxaihiolan-4-yl]cytosine [(-)-OddC; Grove, K. L., et al, <i>Cancer Res</i>., <u>55</u>, 3008-3011, <b>1995],</b> 2',3'-dideoxy-β-L-cystidine (β-L-ddC; Lin, T.S., et al, <i>J. Med</i>. <i>Chem.,</i><u>37</u>, 798-803, <b>1994),</b> 2'fluoro-5-methyl-β-L-arabinofuranosyluracil (L-FMAU; U.S. Pat. No. 5,567,688), 2',3'-dideoxy-2',3'-didehydro-β-L-cystidine (β-L-d4C; Lin, T.S., et al, <i>J. Med. Chem.</i>, <u>39</u>, 1757-1759, <b>1996</b>), 2',3'-dideoxy-2',3'-didehydro-β-L-5-fluorocystidine (β-L-Fd4C; Lin, T.S., et al, <i>J. Med. Chem</i>., <u>39</u>, 1757-1759, <b>1996),</b> L-cyclopentyl carbocyclic nucleosides (Wang, P. et al, <i>Tetrahedron Letts.</i>, <u>38</u>, 4207-4210, <b>1997</b>) and variety of 9-(2'-deoxy-2'-fluoro-β-L-arabinofuranosyl)purine nucleosides (Ma, T.' et al, <i>J. Med. Chem.,</i><u>40</u>, 2750-2754, <b>1997).</b>

Other research <i>on</i> L-nucleosides has <i>also</i> been reported. U.S. Pat. No. 5,009,698, for example, describes the synthesis and use of L-adenosine to stimulate the growth of a plant. WO 92/08727 describes certain L-2'-deoxyuridines and their use for treating viruses. Spadari, S., et al, <i>J. Med. Chem.,</i><u>35</u>, 4214-4220, <b>1992,</b> describes the synthesis of certain L-β-nucleosides useful for treating viral infections including Herpes Simplex Virus Type I. U.S. Pat. No. 5,559,101 describes the synthesis of α- and β-L-ribofuranosyl nucleosides, processes for their preparation, pharmaceutical composition containing them, and method of using them to treat various diseases in mammals. A German patent (De 195 18 216) describes the synthesis of 2'-fluoro-2'-deoxy-L-β-arabinofuranosyl pyrimidine nucleosides. U.S. Pat. Nos. 5,565,438 and 5,567,688 describe the synthesis and utility of L-FMAU. WO Patent 95/20595 describes the synthesis of 2'-deoxy-2'-fluoro-L-β-arabinofuranosyl purine and pyrimidine nucleosides and method of treating HBV or EBV. U.S. Pat. No. 5,567,689 describes methods for increasing uridine levels with L-nucleosides. WO patent 96/28170 describes a method of reducing the toxicity of D-nucleosides by co-administering an effective amount of L-nucleoside compounds.

Finally, Nucleotides and Nucleosides, 12 (2), 215-224 (1993) describes the synthesis of 5-formamido-1-(α-L-arabinofuranosyl)imidazole-4-carboxamide and 4-formamido-1-(α-L-arabinopyranosyl)imidazole-5-carboxamide.

Significantly, while some of the known L-nucleosides have shown potent antiviral activity with lower toxicity profiles than their D-counterparts, none of these L-nucleoside compounds have been shown to posses immunomodulatory properties. Moreover, at present there is no effective treatment for the modulation of the immune system where lymphokine profiles (Th1 and Th2 subsets) have been implicated. Thus, there remains a need for novel L-nucleoside analogs, especially a need for L-nucleoside analogs which modulate the immune system, and most especially L-nucleoside analogs which specifically modulate Th1 and Th2.

BRIEF DESCRIPTION OF THE INVENTION

The present invention is directed to novel L-nucleoside compounds, their therapeutic uses and synthesis.

The novel L-nucleoside compounds are defined in claim 1. Their formmla is depicted in claim 1 as formula III.

In one class of preferred embodiments of the invention, the compound of Formula III comprises a ribofuranosyl moiety, and in a particularly preferred embodiment the compound comprises L-Ribavirin.

In another aspect of the invention, a pharmaceutical composition comprises a therapeutically effective amount of a compound of Formula III or a pharmaceutically acceptable ester or salt thereof admixed with at least one pharmaceutically acceptable carrier.

In yet another aspect of the invention, a compound according to Formula III is used in the treatment of any condition which responds positively to administration of the compound, and according to any formulation and protocol which achieves the positive response. Among other things it is contemplated that compounds of Formula III maybe used to treat an infection, an infestation, a cancer or tumor or an autoimmune disease.

BRIEF DESCRIPTION OF THE FIGURES

<ul id="ul0001" list-style="none" compact="compact"><li>Figure 1 is a schematic representation of synthetic chemical steps which may be used to prepare compounds in the examples section below.</li><li>Figures 2 and 3 are graphical representations of the effect of D-Ribavirin and L-Ribavirin on IL-2 TNFα, IFN-γ, IL-4 and IL-5 levels of activated T-cells.</li><li>Figure 4 is a graphical representation of depicts In another set of experiments the effects of L-Ribavirin on the inflammatory ear response to dinitrofluorobenzene were determined.</li></ul>

DETAILED DESCRIPTION

Where the following terms are used in this specification, they are used as defined below.

The term "nucleoside" refers to a compound composed of any pentose or modified pentose moiety attached to a specific position of a heterocycle or to the natural position of a purine (9-position) or pyrimidine (1-position) or to the equivalent position in an analog.

The term "nucleotide" refers to a phosphate ester substituted on the 5'-position of a nucleoside.

The term "heterocycle" refers to a monovalent saturated or unsaturated carbocyclic radical having at least one hetero atom, such as N, O or S, within the ring each available position of which can be optionally substituted, independently, with, e.g., hydroxy, oxo, amino, imino, lower alkyl, bromo, chloro and/or cyano. Included within this class of substituents are purines, pyrimidines.

The term "purine" refers to nitrogenous bicyclic heterocycles.

The term "pyrimidine" refers to nitrogenous monocyclic heterocycles.

The term "D-nucleosides" that is used in the present invention describes to the nucleoside compounds that have a D-ribose sugar moiety (e.g., Adenosine).

The term "L-nucleosides" that is used in the present invention describes to the nucleoside compounds that have an L-ribose sugar moiety.

The term "L-configuration" is used throughout the present invention to describe the chemical configuration of the ribofwanosyl moiety of the compounds that is linked to the nucleobases. The L-configuration of the sugar moiety of compounds of the present invention contrasts with the D-configuration of ribose sugar moieties of the naturally occurring nucleosides such as cytidine, adenosine, thymidine, guanosine and uridine.

The term "C-nucleosides" is used throughout the specification to describe the linkage type that formed between the ribose sugar moiety and the heterocyclic base. In C-nucleosides, the linkage originates from the C-1 position of the ribose sugar moiety and joins the carbon of the heterocyclic base. The linkage that forms in C-nucleosides are carbon to carbon type.

The term "N-nucleosides" is used throughout the specification to describe the linkage type that formed between the ribose sugar moiety and the heterocyclic base. In N-nucleosides, the linkage originates from the C-1 position of the ribose sugar moiety and joins the nitrogen of the heterocyclic base. The linkage that forms in N-nucleosides are carbon to nitrogen type.

The term "protecting group" refers to a chemical group that is added to, oxygen or nitrogen atom to prevent its further reaction during the course of derivatization of other moieties in the molecule in which the oxygen or nitrogen is located. A wide variety of oxygen and nitrogen protecting groups are known to those skilled in the art of organic synthesis.

The term "lower alkyl" refers to methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, i-butyl or n-hexyl. This term is further exemplified to a cyclic, branched or straight chain from one to six carbon atoms.

The term "aryl" refers to a monovalent unsaturated aromatic carbocyclic radical having a single ring (e.g., phenyl) or two condensed rings (e.g., naphthyl), which can optionally be substituted with hydroxyl, lower alky, chloro, and/or cyano.

The term "heterocycle" refers to a monovalent saturated or unsaturated carbocyclic radical having at least one hetero atom, such as N, O, S, Se or P, within the ring, each available position of which can be optionally substituted or unsubstituted, independently, with e.g., hydroxy, oxo, amino, imino, lower alkyl, bromo, chloro, and/or cyano.

The term "monocyclic" refers to a monovalent saturated carbocyclic radical having at least one hetero atom, such as O, N, S, Se or P, within the ring, each available position of which can be optionally substituted, independently, with a sugar moiety or any other groups like bromo, chloro and/or cyano so that the monocyclic ring system eventually aromatized [e.g., Thymidine; 1-(2'-deoxy-β-D-erythro-pentofuranosyl)thymine].

The term "immunomodulators" refers to natural or synthetic products <i>capable</i> of modifying the normal or aberrant immune system through stimulation or suppression.

The term "effective amount" refers to the amount of a compound of formula (III) which will restore immune function to normal levels, or increase immune function above normal levels in order to eliminate infection.

The compounds of Formula III may have multiple asymmetric centers. Accordingly, they may be prepared in either optically active form or as a racemic mixture. The scope of the invention as described and claimed encompasses the individual optical isomers and non-racemic mixtures thereof as well as the racemic forms of the compounds of Formula III.

The terms "α" and "β" indicate the specific stereochemical configuration of a substituent at an asymmetric carbon atom in a chemical structure as drawn. The compounds described herein are all in the L-furanosyl configuration.

The term "enantiomers".refers to a pair of stereoisomers that are non-superimposable mirror images of each other. A mixture of a pair of enantiomers, in a 1:1 ratio, is a "racemic" mixture.

The term "isomers" refers to different compounds that have the same formula. "Stereoisomers" are isomers that differ only in the way the atoms are arranged in space.

A "pharmaceutically acceptable salts" may be any salts derived from inorganic and organic acids or bases.

<u>Compounds</u>

Compounds according to Formula III have the following structure; <chemistry id="chem0001" num="0001"><img file="EP1027359B9_D0001.tif" /></chemistry> wherein: <ul id="ul0002" list-style="none" compact="compact"><li>X is independently O, S, CH₂ and NR, where R is COCH₃;</li><li>R' and R" are independently selected from H, CN, C(=O)NH₂, NH₂, C(=S)NH₂, C(=NH)NH₂.HCl, C(=NOH)NH₂, C(=NH)OMe, heterocycles, halogens, lower alkyl or lower alkyl aryl;</li><li>R₁ and R₄ are independently selected from H, CN, N₃, CH₂OH, lower alkyl or lower alkyl amines; and</li><li>R₂, R₃, R₅, R₆, R₇ and R₈ are independently selected from H, OH, CN, N₃, halogens, CH₂OH, NH₂, OCH₃, NHCH₃, ONHCH₃, SCH₃, SPh, alkenyl, lower alkyl, lower alkyl amines or substituted heterocycles; such that</li></ul> when R₂ = R₃ = H, then R₇ and R₈ are hydrogens or nothing and wherein lower alkyl is a cyclic, branched or straight claim will one to six carbon atoms.

In compounds of Formula III, R' is preferably carboxamide or CN and R" is hydrogen or halogens; R₁ = R₄ = R₅ = R₇ = R₈ = H and R₂ = R₃ = OH, and preferably X is oxygen.

A particular class of compounds of the invention contemplated herein includes nucleoside analogs having a ribofuranosyl moiety where the sugar has an L-configuration rather than the natural D-configuration. This class includes compounds which contain modified natural nucleic acid bases and/or synthetic nucleoside bases like triazole, 3-cyano-1,2,4-triazole, methyl 1,2,4-triazole-3-carboxylate, or 3-bromo-5-nitro-1,2,4-triazole, and other substituted derivatives of these bases. Compounds of this class may also contain independently certain modifications of the ribofuranosyl moiety.

Especially preferred compounds in this class include L-Ribavirin, 1-β-L-ribofuranosyl-1,2,4-triazole-3-carboxamide, L-Ribavirin is described by Figure I.

Ribavirin (1-β-D-ribafuranosyl-1,2,4-triazole-3-carboxamide) is a monocyclic synthetic D-nucleoside that has been demonstrated activity against variety of viral diseases (Huffman et al, <i>Antimicrob. Agents Chemother</i>., <u>3</u>, 235, 1975; Sidwell et al, <i>Science</i>, <u>177</u>, 705, 1972) and currently undergoing clinical trials in combination with γ-interferon for the treatment of Hepatitis C virus. In the past two decades, a variety of Ribavirin D-nucleoside analogs have been explored and many of them exhibit the exceptional antiviral and antitumor activities. However, no work has been reported on the synthesis of L-isomer of Ribavirin analogs and their biological activity. In single crystal X-ray analysis Ribavirin resemble structurally to guanosine (Prusiner et al., <i>Nature</i>, <u>244</u>, 116, <b>1973</b>). Because of the resemblance of Ribavirin to guanosine, we expected that Ribavirin nucleoside analogs should show similar or superior immune-modulating activity than guanosine analogs (Robins et al, US 5,041,426) in addition to the antiviral activity.

<u>Uses</u>

If is contemplated that the compounds of the present invention will used to treat a wide variety of conditions, and in fact any condition which responds positively to administration of one or more of the compounds. Among other things it is specifically contemplated that compounds of the invention may be used to treat an infection, an infestation, a cancer or tumor or an autoimmune disease.

Infections contemplated to be treated with the compounds of the present invention include respiratory syncytial virus (RSV), hepatitis B virus (HBV), hepatitis C virus (HCV), herpes simplex type 1 and 2, herpes genitalis, herpes keratitis, herpes encephalitis, herpes zoster, human immunodeficiency virus (HTV), influenza A virus, hantann virus (hemorrhagic fever), human papilloma virus (HPV), measles and fungus.

Infestations contemplated to be treated with the compounds of the present invention include protozoan infestations, as well as helminth and other parasitic infestations.

Cancers or tumors contemplated to be treated include those caused by a virus, and the effect may involve inhibiting the transformation of virus-infected cells to a neoplastic state, inhibiting the spread of viruses from transformed cells to other normal cells and/or arresting the growth of virus-transformed cells.

Autoimmune and other diseases contemplated to be treated include arthritis, psoriasis, bowel disease, juvenile diabetes, lupus, multiple sclerosis, gout and gouty arthritis), rheumatoid arthritis, rejection of transplantation, allergy and asthma.

Still other contemplated uses of the compounds according to the present invention include use as intermediates in the chemical synthesis of other nucleoside or nucleotide analogs which are, in turn, useful as therapeutic agents or for other purposes.

In yet another aspect, a method of treating a mammal comprises administering a therapeutically and/or prophylactically effective amount of a pharmaceutical containing a compound of the present invention. In this aspect the effect may relate to modulation of some portion of the mammal's immune system, especially modulation of lymphokines profiles of Th1 and Th2. Where modulation of Th1 and Th2 lymphokines occurs, it is contemplated that . the modulation may include stimulation of both Th1 and Th2, suppression of both Th1 and Th2, stimulation of either Th1 or Th2 and suppression of the other, or a bimodal modulation in which one effect on Th1/Th2 levels (such as generalized suppression) occurs at a low concentration, while another effect (such as stimulation of either Th1 or Th2 and suppression of the other) occurs at a higher concentration.

In general, the most preferred uses according to the present invention are those in which the active compounds are relatively less cytotoxic to the non-target host cells and relatively more active against the target. In this respect, it may also be advantageous that L-nucleosides may have increased stability over D-nucleosides, which could lead to better pharmacokinetics. This result may attain because L-nucleosides may not be recognized by enzymes, and therefore may have longer half-lives.

It is contemplated that compounds according to the present invention will be administered in any appropriate pharmaceutical formulation, and under any appropriate protocol. Thus, administration may take place orally, parenterally (including subcutaneous injections, intravenous, intramuscularly, by intrsternal injection or infusion techniques), by inhalation spray, or rectally, topically and so forth, and in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles.

By way of example, it is contemplated that compounds according to the present invention can be formulated in admixture with a pharmaceutically acceptable carrier. For example, the compounds of the present invention can be administered orally as pharmacologically acceptable salts. Because the compounds of the present invention are mostly water soluble, they can be administered intravenously in physiological saline solution (e.g., buffered to a pH of about 7.2 to 7.5). Conventional buffers such as phosphates, bicarbonates or citrates can be used for this purpose. Of course, one of ordinary skill in the art may modify the formulations within the teachings of the specification to provide numerous formulations for a particular route of administration without rendering the compositions of the present invention unstable or compromising their therapeutic activity. In particular, the modification of the present compounds to render them more soluble in water or other vehicle, for example, may be easily accomplished by minor modifications (salt formulation, esterification, <i>etc.)</i> which are well within the ordinary skill in the art. It is also well within the ordinary skill of the art to modify the route of administration and dosage regimen of a particular compound in order to manage the pharmacokinetics of the present compounds for maximum beneficial effect in patients.

In certain pharmaceutical dosage forms, the pro-drug form of the compounds, especially including acylated (acetylated or other) derivatives, pyridine esters and various salt forms of the present compounds are preferred. One of ordinary skill in the art will recognize how to readily modify the present compounds to pro-drug forms to facilitate delivery of active compounds to a target site within the host organism or patient. One of ordinary skill in the art will also take advantage of favorable pharmacokinetic parameters of the pro-drug forms, where applicable, in delivering the present compounds to a targeted site within the host organism or patient to maximize the intended effect of the compound.

In addition, compounds according to the present invention may be administered alone or in combination with other agents for the treatment of the above infections or conditions. Combination therapies according to the present invention comprise, the administration of at least one compound of the present invention, or a functional derivative thereof and at least one other pharmaceutically active ingredient. The active ingredient(s) and pharmaceutically active agents may be administered separately or together and when administered separately this may occur simultaneously of separately in any order. The amounts of the active ingredient(s) and pharmaceutically active agent(s) and the relative timings of administration will be selected in order to achieve the desired combined therapeutic effect. Preferably the combination therapy involves the administration of one compound of the present invention or a physiologically functional derivative thereof and one of the agents mentioned herein below.

Examples of such further therapeutic agents include agents that are effective for the modulation of immune system or associated conditions such as AZT, 3TC, 8-substituted guanosine analogs, 2',3'-dideoxynucleosides, interleukin II, interferons such as γ-interferon, tucaresol, levamisole, isoprinosine and cyclolignans. Certain compounds according to the present invention may be effective for enhancing the biological activity of certain agents according to the present invention by reducing the metabolism or inactivation of other compounds and as such, are co-administered for this intended effect.

With respect to dosage, one of ordinary skill in the art will recognize that a therapeutically effective amount will vary with the infection or condition to be treated, its severity, the treatment regimen to be employed, the pharmacokinetics of the agent used, as well as the patient (animal or human) treated. Effective dosages may range from 1 mg/kg of body weight, or less, to 25 mg/kg of body weight or more. In general a therapeutically effective amount of the present compound in dosage form usually ranges from slightly less than about 1 mg./kg. to about 25 mg./kg. of the patient, depending upon the compound used, the condition or infection treated and the route of administration. This dosage range generally produces effective blood level concentrations of active compound ranging from about 0.04 to about 100 micrograms/cc of blood in the patient. It is contemplated, however, that an appropriate regimen will be developed by administering a small amount, and then increasing the amount until either the side effects become unduly adverse, or the intended effect is achieved.

Administration of the active compound may range from continuous (intravenous drip) to several oral administrations per day (for example, Q.I.D.) and may include oral, topical, parenteral, intramuscular, intravenous, sub-cutaneous, transdermal (which may include a penetration enhancement agent), buccal and suppository administration, among other routes of administration.

To prepare the pharmaceutical compositions according to the present invention, a therapeutically effective amount of one or more of the compounds according to the present invention is preferably intimately admixed with a pharmaceutically acceptable carrier according to conventional pharmaceutical compounding techniques to produce a dose. A carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral. In preparing pharmaceutical compositions in oral dosage form, any of the usual pharmaceutical media may be used. Thus, for liquid oral preparations such as suspensions, elixirs and solutions, suitable carriers and additives including water, glycols, oils, alcohols, flavouring agents, preservatives, colouring agents and the like may be used. For solid oral preparations such as powders, tablets, capsules, and for solid preparations such as suppositories, suitable carriers and additives including starches, sugar carrier, such as dextrose, mannitol, lactose and related carriers, diluents, granulating agents, lubricants, binders, disintegrating agents and the like may be used. If desired, the tablets or capsules may be enteric-coated or sustained release by standard techniques.

For parenteral formulations, the carrier will usually comprise sterile water or aqueous sodium chloride solution, though other ingredients including those which aid dispersion may be included. Of course, where sterile water is to be used and maintained as sterile, the compositions and carriers must also be sterilized. Injectable suspensions may also be prepared, in which case appropriate liquid carriers, suspending agents and the like may be employed.

<u>Test Results</u>

<i>In vitro and in vivo</i> tests on a compound of Formula III, L-Ribavirin, were performed, and the results are described below.

In a first series of experiments, peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coat following Ficoll-Hypaque density gradient centrifugation of 60 ml blood from healthy donors. T-cells were then purified from the PBMCs using Lymphokwik lymphocyte isolation reagent specific for T-cells (LK-25T, One Lambda, Canoga Park CA). An average yield of 40 - 60 x 10⁶ T-cells were then incubated overnight at 37 °C in 20 - 30 ml RPMI-AP5 (RPMI-1640 medium (ICN, Costa Mesa, CA) containing 20 mM HEPES buffer, pH 7.4, 5 % autologous plasma, 1 % L-ghitamine, 1 % penicillin/streptomycin and 0.05 % 2-mercaptoethanol) to remove any contaminating adherent cells. In all experiments, T-cells were washed with RPMI-AP5 and then plated on 96-well microtitre plates at a cell concentration of 1 x 10⁶ cells/ml.

The T-cells were activated by the addition of 500 ng ionomycin and 10 ng phorbol 12-myristate 13-acetate (PMA) (Calbiochem, La Jolla, CA) and incubated for 48 - 72h at 37 °C. PMA/ionomycin-activated T-cells were treated with 0.5 - 50 µM of either Ribavirin (D-Ribavirin) or L-Ribavirin, or with 250 - 10000 U/ml of a control antiviral, interferon-alpha (Accurate, Westbury, NY) immediately following activation and re-treated 24 h later. T-cells from each plate were used for immunofluorescence analysis and the supernatants used for extracellular cytokine measurements. Following activation, 900 µl cell supernatant from each microplate was transferred to another microplate for analysis of cell-derived cytokine production. The cells are then used in immunofluorescence analyses for intracellular cytokine levels and cytokine receptor expression.

Cell-derived human cytokine concentrations were determined in cell supernatants from each microplate. Activation-induced changes in interleukin-2 (IL-2) levels were determined using a commercially available ELISA kit (R &amp; D systems Quantikine kit, Minneapolis, MN) or by bioassay using the IL-2-dependent cell line, CTLL-2 (ATCC, Rockville, MD). Activation -induced changes in interleukin-4 (IL-4), tumor necrosis factor (TNFα) interleukin-8 (LL-8) (R &amp; D systems (Quantikine kit, Minneapolis, MN) and interferon-gamma (IFN-γ) (Endogen (Cambridge, MA) levels were determined using ELISA kits. All ELISA results were expressed as pg/ml and the CTLL-2 bioassay as counts per minute representing the IL-2-dependent cellular incorporation of ³H-thymidine (ICN, Costa Mesa, CA) by CTLL-2 cells.

Comparison of the effects of D-Ribavirin and L-Ribavirin (expressed as a percentage of activated control) on IL-2 TNFα, IFN-γ, IL-4 and IL-5 levels are presented in Figures 2 and 3.

In another set of experiments the effects of L-Ribavirin on the inflammatory ear response to dinitrofluorobenzene were determined. The results of those experiments are shown in Figure 4.

<u>Synthesis</u>

The compounds according to the present invention may be produced according to synthetic methods which are individually readily known to those of ordinary skill in the art. In general, compounds according to the present invention are synthesized by condensing appropriate nucleoside base with the necessary sugar synthon to give the protected L-nucleoside which on further manipulation and deprotection of the sugar hydroxyl protecting groups will ultimately give rise to nucleoside analog having the desired ribofuranosyl moiety of the L-configuration.

During chemical synthesis of the various compositions according to the present . invention, one of ordinary skill in the art will be able to practice the present invention without undue experimentation. In particular, one of ordinary skill in the art will recognize the various steps that should be performed to introduce a particular substituent at the desired position of the base or a substituent at the desired position on the sugar moiety. In addition, chemical steps which are taken to protect functional groups such as hydroxyl or amino groups, among others, as well as de-protected these same functional groups, will be recognized as appropriate within the circumstances of the syntheses.

The invention is further defined by reference to the following examples, which are intended to be illustrative and not limiting. It will be understood by one of ordinary skill in the art that these examples are in no way limiting and that variations of detail can be made without departing from the spirit and scope of the present invention.

Compounds of the present invention may be prepared in accordance with well known procedures in the art. Particularly useful are the following synthetic schemes.

Scheme 1: Synthesis of ribofuranosyl nucleosides of formula (III): Triazole L-ribofuranosyl nucleosides were prepared by the acid catalyzed fusion procedure (Sato, T., et al, Nippon Kagaku Zasshi, 81, 1440, 1960). Accordingly, the triazoles (<b><u>1</u></b>) were mixed with 1,2,3,5-terra-<u>O</u>-acetyl-L-ribose (<b><u>2</u></b>) and a catalytic amount of bis(p-nitrophenyl)phosphate and heated at 160-165 C for 30 min under reduced pressure to provide the required nucleosides which on further deprotection furnished the triazole L-ribonucleosides (<b><u>3</u></b>) of formula (III). <chemistry id="chem0002" num="0002"><img file="EP1027359B9_D0002.tif" /></chemistry>

Scheme 2: Synthesis of L-ribofuranosyl (R1, R4, R5, R7 and R8, are hydrogens; R2, R3 and R6 are hydroxyl) nucleosides of formula (III): Triazole, L-ribofuranosyl nucleosides of the present invention were prepared by using Vorbruggen procedure involves the treatment of the base (<b><u>4</u></b>) with chlorotrimethylsilane to provide the silyl intermediate which on condensation with the protected ribose (<b><u>5</u></b>) in the presence of stannic chloride in an inert solvent affords the required nucleosides (<b><u>6</u></b>). After condensation the products are deprotected by conventional methods known to those skilled in the art, into compounds of the formula (III). <chemistry id="chem0003" num="0003"><img file="EP1027359B9_D0003.tif" /></chemistry>

Most of compounds of the formula (III) can be prepared by using the above condensation procedure. The required 1,2,3,5-tetra-O-acetyl-L-ribose and 1-O-acetyl-2,3,5-tri-O-benzoyl-Lribose were prepared as shown in Example 2 and Example 6 respectively. The hetero monocyclic bases are commercially available from Aldrich, Fluka, ICN, Acros, Alfa, Lancaster and TCI America or were prepared by following the reported procedure that are available in the literature articles (Robins, R. K., et al, Nucleosides &amp; Nucleotides, 13, 17-76, 1994).

Scheme 4: Preparation of L-arabinofuranosyl nucleosides (R₁, R₂, R₄, R₅ and R₈ are hydrogens; R₃, R₆ and R₇ are hydroxyl): The b-anomers of the arabinosyl L-nucleosides of formulae (I - III) may be prepared by reacting 2,3,5-tri-O-benzyl-L-arabinofuranosyl bromide (<b><u>9</u></b>; Baker, R, et al., <i>J. Org. Chem</i>., <u>26</u>, 4605-4609, <b>1961</b>) and the trimethylsilyl derivative of the base to give the intermediate L-nucleoside (<b><u>10</u></b>). Removal of the blocking groups of <b><u>10</u></b> should afford the required b-L-arabinofuranosyl nucleosides. In the case of pyrrole b-L-arabinonucleosides the sodium salt glycosylation procedure (Revankar, G. R, et al, <i>Nucleosides &amp; Nucleotides</i>, <u>6</u>, 261-264, <b>1987</b>) was followed. <chemistry id="chem0004" num="0004"><img file="EP1027359B9_D0004.tif" /></chemistry>

Scheme 5: Preparation of L-xylofuranosyl nucleosides (R₁, R₃, R₄, R₅ and R₇ are hydrogens; R₂, R₆ and R₈ are hydroxyl): The b-anomers of the xylofuranosyl L-nucleosides of formulae (I-III) may be prepared from 1,2-di-<u>O</u>-acetyl-3,5-di-<u>O</u>-benzyl-L-xylofuranose (<b><u>11</u></b>; Gosselin, G., et aL, <i>J. Heterocyclic Chem.,</i><u>30</u>, 1229-1233, <b>1993</b>) and the appropriate base, by following the method analogous to that described in scheme 4. <chemistry id="chem0005" num="0005"><img file="EP1027359B9_D0005.tif" /></chemistry>

Scheme 6: Preparation of L-2'-deoxyribofuranosyl nucleosides (R₁, R₂, R₄, R₅, R₇ and R₈ are hydrogens; R₃ and R₆ are hydroxyl): The b-anomers of the 2'-deoxyribofuranosyl L-nucleosides of formulae (I - III) may be prepared by reacting 3',5'-Di-<u>O</u>-<i>p</i>-toluyl-2'-deoxyerythro-b-L-pentofuranosyl chloride (<b><u>13</u></b>) (Smejkal, J., et al, <i>Collect. Czec. Chem. Commun</i>. <u>29</u>, 2809-2813, <b>1964</b>) with the silyl derivative of the heterocycles in the presence of Bronsted acid to give exclusively the b-isomers (<b><u>14</u></b>) in good yield (Fujimori, S., et al, <i>Nucleosides &amp; Nucleotides</i>, <u>11</u>, 341-349, <b>1992</b>; Aoyama, H., <i>Bull. Chem. Soc</i>., <u>60</u>, 2073, <b>1987</b>). The same b-L-2'-deoxyribofuranosyl nucleosides were also prepared by the reacting the chloro sugar (<b><u>13</u></b>) with sodium salt of the base (Kazimierczuk, Z., et al, <i>J. Amer. Chem. Soc</i>., <u>106</u>, 6379-6382, <b>1984</b>) in dry acetonitrile. The intermediate (14) on treatment with methanolic ammonia provided the required b-L-2'-deoxyerythro-pertofuranosyl nucleosides. <chemistry id="chem0006" num="0006"><img file="EP1027359B9_D0006.tif" /></chemistry>

Scheme 7: Preparation of L-3'-deoxyribofuranosyl nucleosides (R₁, R₃, R₄, R₅, R₆, R₇ and R₈ are hydrogens; R₂ and R₆ are hydroxyl): The b-anomers of the 3'-deoxyribofuranosyl L-nucleosides of formulae (I - III) may be prepared by reacting 1,2-di-<u>O</u>-acetyl-5-<u>O</u>-benzoyl-3-deoxy-L-erythro-pentose (<b><u>15</u></b>) with the silyl derivative of the heterocycles in the presence of Lewis acid to give the b-isomers (<b><u>16</u></b>), which on deblocking with methanolic ammonia should give b-L-3'-deoxyerythro-pentofuranosyl nucleosides. The same compounds could also be prepared by reacting the corresponding 1-chloro derivative of (<b><u>15</u></b>) with sodium salt of the heterocyclic base, as in the case of 2'-deoxy L-nucleosides described in scheme 6. <chemistry id="chem0007" num="0007"><img file="EP1027359B9_D0007.tif" /></chemistry>

Scheme 8: Preparation of L-2',3'-dideoxyribofuranosyl nucleosides (R₁, R₂, R₃, R₄, R₅, R₇ and R₈ are hydrogens; R₆ is hydroxyl): The b-anomers of the 2',3'-dideoxyribofuranosyl L-nucleosides of formulae (I -III) may be prepared by the treatment of their corresponding 5'-<u>O</u>-triphenylmethyl-2',3'-bis(methanesulfonate)-b-L-ribofuranosyl nucleosides (<b><u>17</u></b>) with sodium hydrogentelluride (Clive, D. L., et al, <i>J. Org. Chem</i>., <u>61</u>, 7426-7437, 1996) in CH₃CN at room temperature as shown below. Finally the trityl group will be removed from (<b><u>18</u></b>) under mild condition to provide the 2',3'-dideoxyribofuranosyl b-L-nucleosides. <chemistry id="chem0008" num="0008"><img file="EP1027359B9_D0008.tif" /></chemistry>

Furthermore, substituted sugars such as 1-bromo-2-deoxy-2-fluoro-3,6-O-benzoyl-L-arabinofuranose (Ma, T., et al, <i>J. Med. Chem</i>., <u>39</u>, 2835-2843, <b>1996</b>) and other modified sugars of L-configuration are known in U.S. Pat. No. 5,473,063; WO 96/13512; WO 96/13498; WO 96/22778; WO 95/20595; U.S. 5,473,063; U.S. 5,567,688; WalczaK, K., et al, <i>Monatsh. fur Chemie</i>, <u>123</u>, 349-354(1992); Wengel, J., et al, J. <i>Org. Chem</i>., <u>56</u>, 3591-3594(1991); Genu-Dellac, C., et al, <i>Tetrahedron Letts</i>., <u>32</u>, 79-82(1991) and Czernecki, S., et al, <i>Synthesis</i>, 783(1991). In addition, preparation of modified sugars and nucleosides of D-configuration are described in U.S. Pat. No. 5,192,749; WO 94/22890; Uteza, V., et al, <i>Tetrahedron</i>, <u>49</u>, 8579-8588(1993); Thrane, H., et al, <i>Tetrahedron</i>, <u>51</u>, 10389-10403(1995); Yoshimura, Y., et al, <i>Nucleosides &amp; Nucleotides</i>, <u>14</u>, 427429 (1993; Lawrence, A. J., et al, <i>J. Org. Chem</i>., <u>61</u>, 9213-9222(1996); Ichikawa, S., et al, <i>J. Org. Chem</i>., <u>62</u>, 1368-1375(1997); EP 0 457 326 A1; U.S. Pat. No. 3,910,885; WO 96/13498 and Karpeisky, M, Y., et al, <i>Nucleic Acids Res. Symposium Series</i>, <u>9</u>, 157 (<b>1981</b>). By applying the synthetic procedures (schemes) that has been described in these articles for the preparation of D-nucleosides, the corresponding modified L-nucleosides could also be achieved.

Other compounds within the scope of the invention can be synthetized using the teachings of the schematics provided herein, as well as the specific examples and other schemes set forth below. In addition to the teachings provided herein, the skilled artisan will readily understand how to make compounds within the scope of the present invention by applying well known techniques such as those described in Nucleic Acid Chemistry, Improved and New Synthetic Procedures, Methods and Techniques, Edited by Leroy B. Townsend and R. Stuart Tipson, John Wiley &amp; Sons, New York (1978 - 1991); Chemistry of Nucleosides and Nucleotides, Edited by Leroy B. Townsend, New York, Plenum Press (1988 -1994) and Nucleosides and Nucleotides as Antitumor and Antiviral Agents, Etited by Chung K. Chu and David C. Baker, New York, Plenum Press (1993). Suitable methods for making substitution within the sugar moiety of the presently claimed compounds are known to those skilled in the art and are described in various publications including: U.S. Pat. No. 5,559,101; U.S. Pat. No. 5,192,749; U.S. Pat. No. 5,473,063; U.S. Pat. No. 5,565,438. Suitable methods for making various heterocyclic compounds and substitution on them are provided in Chemistry of Nucleosides and Nucleotides, Edited by Leroy B. Townsend, New York, Plenum Press, 2, 161-398 (1991) and Chemistry of Nucleosides and in Nucleotides, Edited by Leroy B. Townsend, New York, Plenum Press, 3, 1-535 (1994).

EXAMPLES

The invention can be further understood by referring to the following examples below, wherein the compounds numerals in bold correspond to like numbrered numerals in Figures 1.

EXAMPLE 1

1-

O

-Methyl-2,3,5-Tri-

O

-acetyl-β-L-ribofuranose (

<u>19</u>

)

L-Ribose (15.0 g, 100 mmol) was dissolved in dry methanol (200 mL) and cooled to 0°C. To this cold stirred solution H₂SO₄ (2mL) was added slowly and the reaction mixture stirred at below 20°C for 12 h under argon atmosphere. Dry pyridine (75 mL) was added and evaporated to dryness. Dry pyridine (100 mL) was added and evaporated under reduced pressure an oily residue. This residue was dissolved in dry pyridine (150 mL) and treated with acetic anhydride (50 mL) at 0°C under argon atmosphere. TEA (41 mL) was added, the reaction stirred at 0 °C for I h and at room temperature for 36 h, evaporated to dryness. The residue was dissolved in water (200 mL), solid NaHCO₃ was added slowly to adjust the pH of the solution to 7. The aqueous mixture was extracted in CH₂Cl₂ (250 mL), washed with water (150 mL) and brine (100 mL), dried and concentrated. The oily residue was filtered on a bed of silica gel (200 g), washed with CH₂Cl₂:EtOAc (8:2, 1000 mL). The filtrate was evaporated and the oil was used as such for the next reaction.

EXAMPLE 2

1,2,3,5-Tetra-

O

-acetyl-β-L-ribofuranose (

<u>2</u>

)

The syrup (<b><u>19</u></b>) (29.0 g, 100 mmol) from the above reaction was co-evaporated with dry toluene (2x100 mL) and dried overnight under solid NaOH at room temperature <i>in vacuo</i>. The dried syrup was dissolved in glacial acetic acid (150 mL) and cooled to 0 °C under argon atmosphere. To this cold solution was added acetic anhydride (35 mL) followed by H₂SO₄ (10 mL) very slowly during 15 minute period. The reaction mixture was stirred at room temperature overnight and poured into ice (200 g) with stirring. The mixture was extracted with CHCl₃ (2 X 200 mL) and the organic extract was washed with water (200 mL), sat. NaHCO₃ (200 mL) and brine (150 mL), dried over anhydrous Na₂SO₄ and evaporated to dryness. The syrup 30 g (94%) that obtained was found to be pure enough for glycosylation reactions.

EXAMPLE 3A

Methyl 1-(2,3,5-Tri-

O

-acetyl-β-L-ribofuranosyl)-1,2,4-triazole-3-carboxylate

(<u>20</u>)

A mixture of methyl 1,2,4-triazole-3-carboxylate (0.64 g, 5 mmol), 1,2,3,5-tetra-<u>O</u>-acetyl-β-L-ribofuranose <b>(<u>2</u>)</b> (1.5 g, 4.72 mmol) and bis(p-nitrophenyl)-phosphate (20 mg) were placed in a pear shaped flask and placed in a preheated oil bath at (160-165 °C). The flask was connected to a water aspirator and kept at 160-165 °C (oil bath temperature) under reduced pressure with stirring for 25 min. The reaction mixture was removed, cooled and diluted with EtOAc (150 mL) and sat. NaHCO₃ (100 mL). The product was extracted in EtOAc. The organic extract was washed with water (100 mL) and brine (50 mL), dried and evaporated to dryness. The residue that obtained was purified by flash column of silica gel using CHCl₃→EtOAc as the eluent. The pure fractions were collected and evaporated to dryness to give 1.2 g (66%) of pure product: ¹H NMR (CDCl₃) δ 2.10 (3s, 9H, 3 COC<i>H</i>₃), 3.98 (s, 3H, OC<i>H</i>₃), 4.22 (m, 1H), 4.46 (m, 2H), 5.54 (t, 1H), 5.76 (m, 1H), 6.04 (d, 1H, C_1'<i>H</i>), and 8.38 (s, 1H, C₃<i>H</i>). Anal. Calc. for C₁₅H₁₉N₃O₉ (385.22): C, 46.75; H, 4.97; N,10.91. Found: C, 46.82; H, 4.57; N=10.71.

EXAMPLE 3B

1-β-L-Ribofuranosyl-1,2,4-triazole-3-carboxamide (

<u>21</u>

)

The substrate (<b>20</b>) (1.1 g) was dissolved in CH₃OH/NH₃ at 0 °C and placed in a steel bomb. The bomb was closed and stirred at room temperature for 18 h. The steel bomb was cooled, opened and evaporated to dryness. The residue was tried to crystallization with little ethanol. The product crystallized, but on filtration, the crystals re-absorbed water and became a paste. The crystallization repeated several times. Finally it crystallized from Methanol/ Ethanol mixture. The colorless crystals was filtered, washed with methanol and dried in <i>vacuo</i>. The filtrate was evaporated again which on standing gave further crystals. Total yield 0.5 g (72%); mp: 177-179 °C; [a]_D = +38.33 (c 3 mg/mL H₂O); D form of Ribavirin [a]o = -36.0 (c 3.0 mg/mL H₂O); ¹H NMR (Me₂SO-<i>d</i>₆) δ 3.46 (m, 1H, C_5'<i>H</i>), 3.60 (m, 1H, C_5'<i>H</i>), 3.92 (q, 1H, C_4'<i>H</i>), 4.12 (q, 1H), 4.34 (q, 1H), 4.88 (t, 1H, C₅,O<i>H</i>), 5.20 (d, 1H), 5.58 (d, 1H), 5.80 (d, 1H, C_1'<i>H</i>), 7.60 (bs, 1H, N<i>H</i>), 7.82 (bs, 1H, N<i>H</i>), and 8.82 (s, 1H, C₃<i>H</i>). Anal. Calc. for C₈H₁₂N₄O₅ (244.20): C, 39.34; H, 4.95; N, 22.94. Found: C, 39.23; H, 4.97; N, 22.91.

EXAMPLE 4

2,3-

O

-Isopropylidene-L-ribose (

<u>22</u>

)

To a stirred suspension of L-ribose (30.0 g, 260 mmol) in dry acetone (200 mL) was added iodine (1.27 g, 10 mmol) at room temperature under argon atmosphere. The reaction mixture was stirred for I h ( the solution becomes homogeneous during this period) and quenched with sodium thiosulfate solution (1 M). The solution was evaporated to dryness. The residue was dissolved in CH₂Cl₂ (250 mL), dried over anhydrous MgSO₄, filtered and the solid was washed with CH₂Cl₂ (150 mL). The combined filtrate was evaporated to dryness. The residue was placed on top of silica column (8 x 116 cm) packed in CHCl₃. The column was eluted with CHCl₃ (500 mL), CHCl₃:EtOAc (9:1, 1000 mL) and CHCl₃: EtOAc (7.3. 1500 mL). The pure product eluted in CHCl₃: EtOAc (7:3) was collected and evaporated to give an oily residue 34.5 g (90%). The oily product used as such for the next reaction. ¹H NMR (CDCl₃) δ 1.30 and 1.38 (2s, 6H, isopropylidene CH3), 3.70 (m, 3H), 4.08 (m, 1H), 4.38 (m, 1H), 4.55 (d, 1H), 4.81 (d, 1H) and 5.38 (m, 1H).

EXAMPLE 5

1-Deoxy-1-hydrazinyl-2,3-

O

-isopropylidene-L-ribose (

<u>23</u>

)

A solution of 2,3-<u>O</u>-isopropylidene-L-ribose <b><u>22</u></b> (34.5 g, 182 mmol) in absolute methanol (200 mL) was treated with a solution of anhydrous hydrazine (42.0 g, 1313 mmol) in absolute methanol (100 mL) drop-wise over a period of 30 min and at room temperature under argon atmosphere. The nearly colorless solution was stirred at room temperature and under anhydrous condition for 18 h. The solution was evaporated in <i>vacuo</i> to afford a colorless syrup. The syrup was repeatedly co-evaporated with absolute methanol (5 X 100 m). The resulting syrup was momentarily warmed (70 °C) under vacuum pump pressure (0.1 torr) and then kept at this pressure for drying for 12 h. The yield was 35.0 g (95%). This material was used as such without farther purification for the next step.

EXAMPLE 6

1-

O

-Acetyl-2,3,5-tri-

O

-benzoyl-β-L-ribofuranose (

<u>5</u>

)

To a solution of L-ribose (25.0 g, 166.66 mural) in MeOH (300 mL), was added 25 mL of sat, methanolic hydrogen chloride and stirred at room temperature for 6 h. The reaction was complete after 6 h as indicated by TLC using CH₂Cl₂/MeOH 9:1. After completion of the reaction, dry pyridine (30 mL) was added and the solvents were evaporated. To the residue another 30 mL of pyridine was added and evaporated to dryness. The residue was dissolved in dry pyridine (200 mL) and CH₂Cl₂ (150 mL) then cooled to 0 °C. Benzoyl chloride (96.26 mL, 830.12 mmol) was added drop-wise and stirred at room temperature overnight. TLC using hexane/ethyl acetate (7:3), indicated completion of the reaction. The solvents were evaporated and the residue dissolved in CHCl₃ (300 mL), and washed with H₂O (200 mL) and sat. NaHCO₃ (200 mL), and dried over anhydrous Na₂SO₄. After evaporating the CHCl₃, the residue was co-evaporated with toluene to give an oily residue. The residue was dissolved in AcOH (200 mL), acetic anhydride (85.0 mL; 770.9 mural) and sulfuric acid (4.46 mL; 83.29 mmol). The reaction mixture was stirred at room temperature overnight, after which time TLC (hexane/ethyl acetate 7:3) indicated completion of the reacxion. The solvents were evaporated in <i>vacuo</i> and the residue that obtained was co-evaporated with toluene. The brown residue was triturated with EtOH to give light brown crystals. Filtration of the solid and recrystallization from EtOH gave 1-<u>O</u>-acetyl-2,3,5-tri-<u>O</u>-benzoyl-L(+)-glucofuranose 40.5 g (48.0%) as white crystals: mp 125-125 °C; ¹H NMR (CDCl₃) δ 4.49 (m, 1H, C_5'<i>H</i>), 4.77 (m, 2H, C_4'<i>H</i> and C_5'<i>H</i>), 5.80 (d, 1H), 5.93 (m, 1H, C_2'<i>H</i>), 6.43 (d, 1H, C_1'<i>H</i>, J_1,2=1.5 Hz) and 7.30 - 8.09 (m, 15H, Ph<i>H</i>).

EXAMPLE 7

1-Azido-2,3-isopropylidine-b-L-ribofuranose (

<u>51</u>

)

To a solution 2,3,5-tri-<u>O</u>-benzoyl-1-azido-b-L-ribofuranose (9.0 g, 18.48 mmol) in absolute methanol (60 mL) was added 0.5 M solution of sodium methoxide (10.0 mL, 5.0 mmol). The reaction mixture was stirred at room temperature overnight. TLC of the reaction (hexane/ethyl acetate; 7:3) indicated complete conversion of the starting material to a more polar compound. The reaction mixture was neutralized with dry Dowex 50 H⁺ resin and the resin was removed by filtration. The filtrate was evaporated to dryness and dissolved in water (50 mL). The aqueous layer was extracted with dichloromethane (2x100 mL) to remove methyl benzoate and then the aqueous layer was concentrated in <i>vacuo</i>. The residue was further dried over phosphorous pentoxide and used as such for the next step of the synthesis without further characterization.

The above crude product (3.0 g, 17.14 mmol) was suspended in dry acetone (200 mL) and treated with 1,1-dimethoxypropane (50 mL) and vacuum dried Dowex 50 H⁺ (5.0 g) resin. The reaction mixture was stirred at room temperature for 2 h and filtered and the resin was washed with dry acetone (100 mL). The filtrate was evaporated to dryness. The residue was purified by flash chromatography over silica gel using CH₂Cl₂ → EtOAc as the eluent. The pure fractions were pooled and concentrated to give 3.60 g (97%) of product as oil: ¹H NMR (CDCl₃) d 1.44 and 1.27 (2s, 6H, isopropylidene C<i>H</i>₃), 2.70 (br s, 1H, C_5'O<i>H</i>, exchangeable), 3.66 (m, 2H, C_5'<i>H</i>), 4.34 (m, 1H, C_4'<i>H</i>), 4.46 (d, 1H, C_3'<i>H</i>), 4.72 (d, 1H, C_2'<i>H</i>) and 5.50 (s, 1H, C_1'<i>H</i>).

EXAMPLE 8

1-Azido-2,3-

O

-isopropylidine-5-

O

-tert-butyldimethylsilyl-b-L-ribofuranose (

<u>52</u>

)

To a solution of 1-azido-2,3-<u>O</u>-isopropylidine-b-L-ribofuranose (4.20 g, 20 mmol) in dry DMF (25 mL) was added imidazole (2.38 g, 35.0 mmol) and    <i>tert</i>-butyldimethylsilyl chloride (4.50 g, 30.0 mmol). The reaction mixture was stirred at room temperature under argon atmosphere overnight. TLC of the reaction mixture after 16 h indicated complete conversion of the starting material to the product. The solvent was removed in <i>vacuo</i> and the residue dissolved in dichloromethane (200 mL). The organic layer is washed with water (100 mL), satd. sodium bicarbonate (100 mL) and brine (100 mL), dried over sodium sulfate and concentrated to an oily product. Further purification by silica gel flash column chromatography using hexane/ethyl acetate (9:1) gave 6.22 g (94%) of the titled compound as oil: ¹H NMR (CDCl₃) d 0.07 (s, 6H), 0.9 (s, 9H), 1.27 and 1.47 (2s, 6H, isopropylidene C<i>H</i>₃), 3.66 (m, 2H, C_5'<i>H</i>), 4.34 (m, 1H, C_4'<i>H</i>), 4.46 (d, 1H, C_3'<i>H</i>), 4.72 (d, 1H, C_2'<i>H</i>) and 5.50 (s, 1H, C_1'<i>H</i>).

EXAMPLE 9

1-Amino-2,3-

O

-isopropylidine-5-

O

-tert-butyldimethylsilyl-β-L-ribofuranose

(<u>53</u>)

To a mixture of 1-azido-2,3-<u>O</u>-isopropylidine-β-L-ribofuranose (6.0 g, 18 mmol) and Pd/C (0.25 g) in MeOH (50 mL) was hydrogenated at 50 psi on a parr hydrogenator overnight. The reaction mixture was filtered and the catalyst washed with methanol(20 mL). The combined filtrate was evaporated to dryness and dried over P205 at vacuo overnight and used as such for the next reaction without characterization. Yield 5.0 g (90%).