Nova Patents
EP0341444A3

Rapid mutational analysis method

Abstract

A rapid mutational analysis method for mapping protein epitopes is disclosed. This method has been used to identify the binding sites for 16 anti-CD2 and anti-CD4 monoclonal antibodies. The powerful, rapid, and simple method of the present invention allows isolation of a very large number of mutants, and is applicable to any surface protein for which a cDNA and monoclonal antibodies are available. The present method is especially useful in ligand binding site studies for the design of new ligands and drugs.

Term

Term ended

Projected expiry passed 13 April 2009, 17.4 years ago.

  1. Priority
  2. Filed
  3. Published
  4. Projected expiry
  5. Today

Every citation, both waysCites: the store holds 4 of 5

Citations
DocumentRelationOfficeCited during
EP0160486A2CitesEuropean Patent Office (EPO)Search report
US4677056ACitesUnited States of AmericaSearch report
US4677061ACitesUnited States of AmericaSearch report
WO8604921A1CitesWorld Intellectual Property Organization (WIPO)Search report
CHEMICAL ABSTRACTS, Vol. 106, 1986, page 360, Ref. No. 3687x, Columbus, Ohio, US; J.S. McDOUGAL et al.: "Binding of the human retrovirus HTLV-III/LAV/ARV/HIV to the CD4(T4) molecule: conformation dependence, epitope mapping, antibody inhibition and potential for idiotypic mimicry", & JOURNAL OF IMMUNOLOGY, 1986, 137 (9), 2937-44, whole Abstract.Non-patentSearch report
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE OF THE USA, Vol. 75, No. 6, June 1978, pages 2644-2648, Washington, D.C., US; L.J. WYSOCKI et al.: "Planning for lymphocytes: A method for cell selection", page 2645, column 1, lines 10-39.Non-patentSearch report
CELL, Vol. 50, September 11, 1987, pages 975-985, Boston, MA, US; L.A. LASKY et al.: "Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor", whole article.Non-patentSearch report
JOURNAL OF NEUROSCIENCE RESEARCH, Vol. 18, 1987, pages 102-107, Alan R. Liss, Inc., New York, US; P. SACERDOTE et al.: "Vasoactive intestinal peptide 1-12: A ligand for the CD4 (T4)/human immunodeficiency virus receptor", whole article.Non-patentSearch report