Nova Patents
EP0333056A2

Method for inhibiting food-borne human pathogens and preventing microbial spoilage in refrigerated food using a lactobacillus.

Abstract

A method for inhibiting food-borne pathogen and spoilage microorganisms in processed foods using a Lactobacillus sp. which produces an antimicrobial substance at refrigeration temperatures is described. The method is particularly effective in inhibiting gas producing heterofermentative spoilage microorganisms, mold, food-borne pathogenic microorganisms, (Listeria and Salmonella) and psychrotrophic microorganisms which can occur in processed foods.

EP0333056A2, drawing sheet 1
Sheet 1 of 4

Term

Term ended

Projected expiry passed 10 March 2009, 17.5 years ago.

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23 claims: 2 independent, 21 dependent

  1. 1
    -1- A method for inhibition of food-borne human pathogen or spoilage microorganisms in a refrigerated processed food which comprises:(a) inoculating the processed food following any heat treatment of the food which would inactivate the cells with a number of cells of a Lactobacillus which remain viable during refrigerated storage and which produce an antimicrobial substance and to inhibit the microorganisms;and (b) storing the food at refrigeration temperatures which prevent the number of the cells from increasing significantly except during transient temperature abuse to thereby inhibit the microorganisms.
  2. 2
    -2- The method of Claim 1 wherein the Lactobacillus is selected from the group consisting of Lactobacillus casei subspecies alactosus NRRL-B-12344 and Lactobacillus sp. NRRL-B-18261.
  3. 3
    -3- The method of Claim 1 wherein the Lactobacillus is Lactobacillus sp. NRRL-B-18261.
  4. 4
    -4- The method of Claim 1 wherein the refrigeration temperatures are between about 1°C and 12°C.
  5. 5
    -5- A method for inhibition of food-borne pathogen or spoilage microorganisms in refrigerated processed foods which comprises:(a) inoculating the processed food following any heat treatment which would inactivate the cells with a number of cells of a Lactobacillus which remains viable during refrigerated storage and which produce an antimicrobial substance;and (b) storing the food at refrigeration temperatures which prevent the number of the added Lactobacillus cells from increasing significantly except during transient temperature abuse, wherein the spoilage microorganisms are inhibited without fermenting the food for at least twice as long as a control without the cells.
  6. 6
    -6- The method of Claim 5 wherein the Lactobacillus is selected from the group consisting of Lactobacillus casei subspecies alactosus NRRL-B-12344 and Lactobacillus sp. NRRL-B-18261.
  7. 7
    -7- The method of Claim 5 wherein the spoilage microorganisms comprise heterofermentative lactobacilli.
  8. 8
    -8- The method of Claim 5 wherein the heterofermentative spoilage microorganisms are selected from the group consisting of Lactobacillus fermentum or Lactobacillus bifermentans .
  9. 9
    -9- The method of Claim 5 wherein the food is selected from the group consisting of hard or soft cheeses.
  10. 10
    -10- The method of Claim 5 wherein the food is or contains sauce.
  11. 11
    -11- The method of Claim 5 wherein the food is a salad dressing.
  12. 12
    -12- The method of Claim 5 wherein the food contains milk or a milk derivative.
  13. 13
    -13- The method of Claim 5 wherein the food is a prepared salad.
  14. 14
    -14- The method of Claim 5 wherein the food is or contains meat.
  15. 15
    -15- The method of Claim 5 wherein the food is or contains cheese.
  16. 16
    -16- The method of Claim 5 wherein the food is or contains Cottage cheese.
  17. 17
    -17- The method of Claim 5 wherein the food is stored at a temperature of between about 1° and 12°C.
  18. 18
    -18- The method of Claim 5 wherein the food is provided in a sealed package.
  19. 19
    -19- The method of Claim 1 wherein the food is inoculated with between about 10² and 10⁸ cells of the Lactobacillus per gram of the food.
  20. 20
    -20- The method of Claim 5 wherein the food is a cheese which can be cracked by gas production.
  21. 21
    -21- The method of Claim 1 wherein the Lactobacillus is selected from the group consisting of Lactobacillus casei subspecies alactosus NRRL-B-12344 and Lactobacillus sp. NRRL-B-18261 and wherein the food is inoculated with between about 10² and 10⁸ cells of the Lactobacillus per gram of the food.
  22. 22
    -22- The method of Claim 21 wherein the temperature is less than about 12°C.
  23. 23
    -23- The method of Claim 17 wherein the temperature is between about 1°C and 12°C.
Independent claims23