EP0246824A3

Biological cryoprotection

Abstract

Biological samples, especially mammalian cells, can be frozen, stored and thawed with increased percentage viability by freezing in an aqueous medium in the presence specifically of organic solids, desirably cholesterol, which cause nucleation at or near the freezing point of the medium thereby diminishing damage. The medium desirably also contains a cryoprotectant eg DMSO or glycerol.

Term

Term ended

Projected expiry passed 15 May 2007, 19.4 years ago.

16 members in 9 offices

This recordMember, by publication datePriority claim

Priority claims4

Priority claims
Document	Office	Kind	Date
8611894	United Kingdom	A	1986-05-15
8611894	United Kingdom	–	1986-05-15
8611894	–	–	–
GB19860011894	–	–	–

Members16

Family members, oldest first
Document	Office	Kind
GB8611894D0	United Kingdom	D0	1986-06-25
DK252087D0	Denmark	D0	1987-05-18
DK252087A	Denmark	A	1987-11-16
AU7299087A	Australia	A	1987-11-19
EP0246824A2	European Patent Office (EPO)	A2	1987-11-25
CN87104158A	China	A	1988-02-17
JPS63287479A	Japan	A	1988-11-24
NZ220324A	New Zealand	A	1990-03-27
EP0246824A3This record	European Patent Office (EPO)	A3	1990-06-06
AU598260B2	Australia	B2	1990-06-21
CN1019815B	China	B	1992-12-30
EP0246824B1	European Patent Office (EPO)	B1	1994-03-23
AT103137T	Austria	T	1994-04-15
ATE103137T1	Austria	T1	1994-04-15
DE3789401D1	Germany	D1	1994-04-28
DE3789401T2	Germany	T2	1994-08-18

42 legal events, as 3 offices reported them to INPADOC

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Events

Legal events, newest first
	Event	Code	Office
2005-05-15	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1997-04-04	Notification of lapseLapsedST	ST	FR
1997-02-03	Nl: lapsed or anulled due to non-payment of the annual feeLapsedNLV4	NLV4	EP
1997-02-01	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1997-01-31	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1997-01-08	Gb: european patent ceased through non-payment of renewal feeCeasedGBPC	GBPC	EP
1996-12-01	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1996-05-15	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1995-05-31	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1995-05-15	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1995-05-11	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1995-05-10	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1995-05-04	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1995-03-15	No opposition filedOpposition26N	26N	EP
1995-01-26	No opposition filed within time limitOppositionORIGINAL CODE: 0009261PLBE	PLBE	EP
1995-01-26	Information on the status of an ep patent application or granted ep patentGrantedSTATUS: NO OPPOSITION FILED WITHIN TIME LIMITSTAA	STAA	EP
1994-10-03	Lu: last paid annual feeEPTA	EPTA	EP
1994-08-05	Fr: translation filedET	ET	EP
1994-07-31	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1994-07-04	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1994-06-30	Patent ceasedCeasedPL	PL	CH
1994-06-22	It: translation for a ep patent filedITF	ITF	EP
1994-06-22	It: translation for a ep patent filedITF	ITF	EP
1994-05-31	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1994-05-17	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1994-05-16	Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP	PGFP	EP
1994-04-28	Corresponds to:REF	REF	EP
1994-03-23	Designated contracting statesAK	AK	EP
1994-03-23	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1994-03-23	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1994-03-23	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1994-03-23	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1994-03-23	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1994-03-23	Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25	PG25	EP
1994-03-23	Corresponds to:REF	REF	EP
1994-02-04	(expected) grantORIGINAL CODE: 0009210GRAA	GRAA	EP
1991-10-16	First examination report despatched17Q	17Q	EP
1991-01-30	Request for examination filed17P	17P	EP
1990-06-06	Designated contracting statesAK	AK	EP
1990-04-17	Search report despatchedORIGINAL CODE: 0009013PUAL	PUAL	EP
1987-11-25	Designated contracting statesAK	AK	EP
1987-10-10	Public reference made under article 153(3) epc to a published international application that has entered the european phaseORIGINAL CODE: 0009012PUAI	PUAI	EP

Biological cryoprotection

Abstract

Term

Priority claims4

Members16

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Events

Numbers

Titles3

Classification

Designated states13