Pyrazolopyrimidine herbicides.

0001This invention concerns herbicidal pyrazolopyrimidine derivatives, processes for their preparation, and compositions containing them.

0002In one aspect, the invention provides the pyrazolopyrimidine derivatives of the formula: <chemistry id="chem0001" num="0001"><img file="EP0244097A2_D0001.tif" /></chemistry>and salts thereof, where: <ul id="ul0001" list-style="none"><li>R and R², which may be the same or different, each represent hydrogen, hydroxy, halo, substituted or unsubstituted alkyl, alkoxy, alkylthio, alkenyl, alkynyl or phenyl, or heterocyclyl;</li><li>_R⁶ represents hydrogen, halo, cyano, carboxy, alkoxycarbonyl, or sustituted or unsubstituted alkyl or carbamoyl; or R and one of R¹and R² together represent an alkylene chain of 3 or 4 carbon atoms;</li><li>R³ represents hydrogen, alkyl, aryl, aralkyl, carboxy, substituted or unsubstituted alkoxycarbonyl, carbamoyl or thiocarbamoyl, cyano or nitro;</li><li>X represents -NR-SO₂-, -SO₂-O-, -SO₂-NR- or -S(O) -CR'R"-. where n is O. 1 or 2 and R. R' and R" each represent hydrogen, or substituted or unsubstituted alkyl, alkenyl. alkynyl, alkoxycarbonyl, acyl, alkylsulphonyl or arylsulphonyl; and</li><li>_R⁴ represents hydrogen, heterocyclyl, or a substituted or unsubstituted alkyl or aryl group.</li></ul>

0003When R . R or R represents halo, it is preferably chloro or bromo.

0004When R¹ or R² represents or contains an alkyl group. that group is preferably of 1 to 6 carbon atoms, especially of 1 to 4 carbon atoms. Specific preferred unsubstituted alkyl or alkyl-containing groups which R¹, and R² may represent include methyl, ethyl, n-propyl, isopropyl. n-butyl, t-butyl, methoxy, ethoxy, n-propoxy, methylthio and ethylthio. The alkyl group may if desired be substituted, for example by one or more halogen atoms, eg fluorine, chlorine or bromine, or by alkoxy groups of 1 to 4 carbon atoms, eg methoxy or ethoxy. Specific preferred sustituted alkyl-containing groups which _R¹, and _R² may represent include chloromethyl, bromomethyl, dichloromethyl, trifluoromethyl, difluoromethoxy, difluoromethylthio, methoxyethyl and ethoxyethyl.

0005When R¹ or R² represents an alkenyl or alkynyl group, that group is preferably of 2 to 6 carbon atoms, for example allyl, vinyl or propargyl. Any such alkenyl or alkynyl group is preferably unsubstituted.

0006When _R¹ or R² represents a phenyl group, it is preferably substituted, for example by one or more halogen atoms, eg fluorine, chlorine or bromine, nitro groups, substituted or unsubstituted amino groups (eg alkylamino, dialkylamino or acylamino groups, especially where the alkyl moiety has from 1 to 4 carbon atoms), cyano groups, or alkyl or alkoxy groups of 1 to 4 carbon atoms, eg methyl, ethyl, methoxy or ethoxy.

0007When R¹, R or R represents a heterocyclic group, that group is preferably a monocyclic group of ₅ or 6 ring atoms which contains at least one atom of nitrogen, oxygen or sulphur. Preferred heterocyclic groups include pyridinyl, pyrrolyl, piperidinyl, morpholinyl and thienyl groups.

0008_R¹ and _R² independently preferably represent hydrogen, hydroxy, methyl, trifluoromethyl or chloro.

0009When R⁶ represents or contains an alkyl group, that group is preferably of 1 to 6 carbon atoms, especially of 1 to 4 carbon atoms. Specific preferred groups which R⁶ may represent include methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, carboxy, methoxycarbonyl, ethoxycarbonyl, carbamoyl, methylcarbamoyl and dimethylcarbamoyl.

0010_R⁶ preferably represents hydrogen, chloro or methyl, or together with R² represents a trimethylene chain.

0011When R represents or contains an alkyl group, that group is preferably of 1 to 6 carbon atoms, especially of 1 to 4 carbon atoms. Specific preferred groups containing unsubstituted alkyl moieties which R³ may represent include methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, methoxycarbonyl, ethoxycarbonyl, isopropoxycarbonyl, n-butoxycarbonyl, methylcarbamoyl and dimethylcarbamoyl. The alkyl group may if desired be substituted, for example by one or more halogen atoms, eg fluorine, chlorine or bromine, or by alkoxy groups of 1 to 4 carbon atoms, eg methoxy or ethoxy. Specific preferred groups containing substituted alkyl moieties which R³ may represent include chloromethyl, bromomethyl, dichloromethyl, trifluoromethyl and ethoxyethoxycarbonyl.

0012When R³ represents or contains an aryl or aralkyl group, it preferably represents or contains a phenyl or benzyl group, which is desirably substituted by one or more alkyl or alkoxy groups of 1 to 4 carbon atoms, halogen atoms, nitro groups or substituted or unsubstituted amino groups (eg alkylamino or dialkylamino groups, especially where the alkyl moiety is of 1 to 4 carbon atoms).

0013_R³ preferably represents carboxy, substituted or unsubstituted alkoxycarbonyl of 2 to 5 carbon atoms (for example methoxycarbonyl or ethoxycarbonyl), carbamoyl or substituted carbamoyl (for example hydrazinocarbonyl or alkyl-substituted hydrazinocarbonyl).

0014When _R⁴ represents an alkyl group, that group is preferably of 1 to 6 carbon atoms, especially of 1 to ₄ carbon atoms. Specific preferred unsubstituted alkyl groups which R⁴may represent include methyl, ethyl, n-propyl, isopropyl. n-butyl and t-butyl. The alkyl group may if desired be substituted, for example by one or more halogen atoms, eg fluorine, chlorine or bromine, or by alkoxy groups of 1 to 4 carbon atoms, eg methoxy or ethoxy. Specific preferred substituted alkyl groups which _R⁴may represent include chloromethyl, bromomethyl, dichloromethyl, trifluoromethyl, methoxyethyl and ethoxyethyl.

0015_R⁴ preferably represents a phenyl group substituted by one or more chlorine atoms, methyl groups, alkoxycarbonyl groups (especially of 2 to 5 carbon atoms), nitro groups or substituted or unsubstituted amino groups (especially alkylamino or dialkylamino groups in which the alkyl moieties are of 1 to 4 carbon atoms).

0016When R, R' or R" represents or contains alkyl, that moiety is preferably of 1 to 6 carbon atoms, especially of 1 to 4 carbon atoms. Specific preferred groups which R, R'and R" may represent include methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, acetyl, methoxycarbonylmethyl, propanoyl, methoxycarbonyl, ethoxycarbonyl, methylsulphonyl, ethylsulphonyl and benzenesulphonyl.

0017When R, R' or R" represents alkenyl or alkynyl, it is preferably of 2 to 6 carbon atoms, for example vinyl, allyl or propargyl.

0018X preferably represents -S0₂-NH-.

0019Preferred salts of the compounds of formula I are those of the compounds where X represents -SO₂-NR-, particularly alkali-metal salts, eg sodium or potassium salts, ammonium salts, trialkylammonium salts, and salts with heterocyclic bases, eg the pyridinium salts.

0020In a particularly preferred group of compounds of formula I, one of R¹ and R² represents methyl and the other represents hydrogen or methyl, R⁶ is hydrogen. _R³ is methoxycarbonyl, ethoxycarbonyl, carbamoyl, methylcarbamoyl or dimethylcarbamoyl, X is -SO₂NH-, and R⁴ is phenyl substituted by one or more fluorine, chlorine or bromine atoms, alkoxycarbonyl groups of 2 to ₅ carbon atoms, or methyl or nitro groups.

0021Especially preferred compounds according to the invention are those of the Examples provided hereinafter though particular mention may be made of methyl 2-(2,6-dichlorophenylsulphamoyl)-5.7-dimethylpyrazolo-[1,5-a]pyrimidine-3-carboxylate and methyl 2-(2.6-dichloro- phenylsulphamoyl)-5-methyl-7-trifluoromethylpyrazolo-[l,5-a]pyrimidine-3-carboxylate.

0022In another aspect, the invention provides a process for the preparation of a pyrazolopyrimidine derivative of formula I by reacting an aminopyrazole of the formula: <chemistry id="chem0002" num="0002"><img file="EP0244097A2_D0002.tif" /></chemistry>where _R³, _R⁴_{. R}and R' are as defined hereinbefore, in a suitable solvent medium, with a diketone of the formula: <chemistry id="chem0003" num="0003"><img file="EP0244097A2_D0003.tif" /></chemistry>where _R¹. R and R are as defined hereinbefore, to give the desired compound.

0023The solvent medium employed is preferably an alkanol such as ethanol, or acetic acid, and the reaction is desirably carried out with heating, eg to reflux.

0024The compounds of formula I in which X represents -SO₂-NR- or -SO₂-O- may be prepared by a process in which a pyrazolopyrimidine sulphonyl halide of the formula: <chemistry id="chem0004" num="0004"><img file="EP0244097A2_D0004.tif" /></chemistry> where R , R , R and R are as defined hereinbefore, and Hal represents halogen, is reacted in the presence of a base with a compound of the formula _R⁴_NHR or R⁴OH where _Rand R⁴are as defined hereinbefore to give the desired compound where X is respectively -SO₂NR- or -SO₂O-.

0025The reaction is conveniently effected at room temperature or with heating, eg from 10 to 100°C, and in a suitable solvent medium, for example pyridine.

0026The base is preferably an organic base, especially a tertiary orgaic base, for example 4-dimethylaminopyridine.

0027The compounds of formula IV may themselves be prepared by reacting a compound of formula I wherein _X-R⁴ represents benzylthio with the appropriate halogen or sulphuryl halide in a suitable solvent medium, to give the desired compound.

0028The reaction is desirably effected with cooling to less than ambient temperature, eg to a temperature of -10°C to 5°C.

0029The compounds of formula I where X represents -NR-SO₂- may alternatively be prepared by reaction of a pyrazolopyrimidine amine of the formula: <chemistry id="chem0005" num="0005"><img file="EP0244097A2_D0005.tif" /></chemistry> where R¹. R², R , R and R are as defined hereinbefore in the presence of a base with a sulphonyl halide of the formula R⁴SO₂Hal where R⁴ is as defined herinbefore and Hal represents halogen to give the desired compound.

0030In turn, the pyrazolopyrimidine amines of formula V may be prepared by a process in which a diaminopyrazole of the formula: <chemistry id="chem0006" num="0006"><img file="EP0244097A2_D0006.tif" /></chemistry>where each R is the same and is as defined hereinbefore and R³ is as defined hereinbefore is reacted with a diketone of the formula R¹COCHR⁶COR² where R¹. R and R⁶ are as defined hereinbefore to give the desired compound.

0031The reaction is desirably effected in a suitable solvent medium and in the presence of a base catalyst.

0032The solvent employed may for example be an alkanol such as ethanol, and the base catalyst employed is suitably an amine, eg triethylamine.

0033The diamines of formula VI are either known compounds or may be prepared by methods known per se.

0034Salts of the compounds of formula I may be prepared by reaction thereof with a suitable base containing the desired cation, eg the appropriate trialkylamine.

0035The compounds of formula I may also be interconverted by methods known per se. For example, the compounds of formula I in which X represents a group -S0₂NR where R is other than hydrogen, may in general be prepared from the corresponding compounds of formula I where R is hydrogen by reaction thereof in the presence of a strong base, for example sodium hydride, with a halide of formula RHal.

0036The compounds of formula I where X represents -S(O)_n-CRR¹- where n is 1 or 2 may be prepared from the corresponding compounds of formula I where n is 0 by selective oxidation techniques known per se.

0037Carboxy groups present in the compounds of formula I may be esterified, ester groups present may be hydrolysed to give the corresponding acids, and cyano or ester groups may be converted to amido or substituted amido groups. Such transformations, as well as many others, may be effected by techniques well-known to those skilled in the art, and various examples of such transformations are provided hereinafter.

0038The compounds of formula I are herbicidally-active against a wide range of broad-leaved and grassy weeds, but are comparatively safe to certain crop species. They may thus be of use as selective herbicides, particularly in the control of a range of weeds in cereals or other crops, eg wheat, barley, maize, soya beans, oilseed rape, cotton or sugar beet.

0039In another aspect, the invention provides a herbicidal composition which comprises one or more compounds of the invention in association with a suitable carrier and/or surface active agent.

0040The compositions usually contain from 0.01 to 99% by weight of the present compounds, and are normally produced initially as concentrates containing from 0.5 to 99%, preferably from 0.5 to 85%, and especially from 10 to 50% by weight thereof. Such concentrates are diluted if necessary before application to the locus to be treated such that the active ingredient comprises from 0.01 to 5% by weight of the formulation applied.

0041The carrier may be water, in which case an organic solvent may also be present, though this is not usually employed. A flowable suspension concentrate may be formed by grinding the compound with water, a wetting agent and a suspending agent, e.g. xanthan gum.

0042The carrier may alternatively be a water immiscible organic solvent, e.g. a hydrocarbon which boils within the range 130-270°C, e.g. xylene, in which the compound is dissolved or suspended. An emulsifiable concentrate containing a water immiscible solvent may be formed with a surface active agent so that the concentrate acts as a self-emulsifiable oil on admixture with water.

0043The carrier may alternatively be a water-miscible organic solvent e.g. 2-methoxy ethanol, methanol, propylene glycol, diethylene glycol, diethylene glycol monoethyl ether, methylformamide or dimethylformamide.

0044The carrier may alternatively be a solid, which may be finely divided or granular. Examples of suitable solids are limestone, clays, sand, mica, chalk, attapulgite, diatomite, perlite, sepiolite, silicas, silicates, lignosulphonates and solid fertilizers. The carrier can be of natural or synthetic origin or can be modified natural material.

0045Wettable powders soluble or dispersible in water may be formed by admixing the compound in particulate form with a particulate carrier or spraying molten compound on to the particulate carrier, admixing a wetting agent and a dispersing agent and finely grinding the whole powder mixture.

0046An aerosol composition may be formed by admixing the compound with a propellant, e.g. a polyhalogenated alkane such as dichlorofluoromethane, and suitably also with a solvent.

0047The term 'surface active agent' is used in the broad sense to include materials variously called emulsifying agents, dispersing agents and wetting agents. Such agents are well known in the art.

0048The surface active agents used may comprise anionic surface active agents, for example mono- or di-esters of phosphoric acid with a fatty alcohol ethoxylate, or salts of such esters, fatty alcohol sulphates such as sodium dodecyl sulphate, ethoxylated fatty alcohol sulphates, ethoxylated alkylphenol sulphates, lignin sulphates, petroleum sulphonates, alkylaryl sulphonates such as alkyl-benzene sulphonates or lower alkylnaphthalene sulphonates, salts of sulphonated naphthaleneformaldehyde condensates, salts of sulphonated phenolformaldehyde condensates, or more complex sulphonates such as the amide sulphonates, e.g. the sulphonated condensation product of oleic acid and N-methyl taurine or the dialkyl sulphosuccinates e.g. the sodium sulphonate of dioctyl succinate.

0049The surface active agents may also comprise non-ionic agents, for example condensation products or fatty acid esters, fatty alcohols, fatty acid amides or alkyl-substituted phenols with ethylene oxide, fatty esters of polyhydric alcohol ethers e.g. sorbitan fatty acid esters, condensation products of such esters with ethylene oxide e.g. polyoxyethylene sorbitan fatty acid esters, block copolymers of ethylene oxide and propylene oxide, acetylenic glycols such as 2,4,7,9-tetramethyl-5- decyn-4,7-diol, or ethoxylated acetylenic glycols.

0050The surface active agents may also comprise cationic agents, for example alkyl- and/or aryl-substituted quaternary ammonium compounds such as cetyl trimethylammonium bromide, or ethoxylated tertiary fatty amines.

0051Preferred surface active agents include ethoxylated fatty alcohol sulphates, lignin sulphonates, alkyl-aryl sulphonates, salts of sulphonated naphthaleneformaldehyde condensates, salts of sulphonated phenolformaldehyde condensates, sodium oleoyl N-methyltauride, dialkyl sulphosuccinates, alkyl phenol ethoxylates, and fatty alkyl ethoxylates.

0052The present active compounds, especially those of the Examples provided hereinafter, and particularly methyl 2-(2,6-dichlorophenylsulphamoyl)-5,7-dimethylpyrazolo-[l,5-a]pyrimidine-3-carboxylate and methyl 2-(2.6-dichloro- phenylsulphamoyl)-5-methyl-7-trifluoromethylpyrazolo-[1,5-a]pyrimidine-3-carbaxylate, may be admixed with another pesticide, eg a herbicide, fungicide or insecticide, or a plant growth regulator, particularly another herbicide. Suitable further herbicides include trietazine, linuron, MCPA, dichlorprop, isoxaben, diflufenican, metolachlor, fluometuron, oxyfluorfen, fomesafen, bentazone, prometryne, norflurazon, chlomazone, EPTC, imazaquin, and especially isoproturon, methabenzthiazuron, trifluralin, ioxynil, bromoxynil, benazolin, mecoprop, fluroxypyr, alachlor, acifluorfen, lactofen, metribuzin and pendimethalin.

0053The present compounds may be applied to plants, the soil, land or aquatic areas, and particularly to a locus at which a crop is growing or is about to grow. The compounds are active both pre- and post-emergence.

0054The invention is illustrated by the following Examples, in which Me = methyl, Et = ethyl, Ph = phenyl and cyhex = cyclohexyl.

Preparative Example A

Methyl 2-benzylthio-5,7-dimethylpyrazolo[1,5-a]pyrimidine--3-carboxylate (Compound Al)

(a) Methyl 3,3-di(benzylthio)-2-cyanoacrylate

0055Sodium (51.8g) was dissolved in methanol (820 ml), and 375 ml of this solution were treated with methyl cyanoacetate (123.8g). The temperature fell from 20°C to about 15°C during the addition. Carbon disulphide (37.5 ml) was added, the temperature rising to about 40°C. The mixture was cooled to 20°C and was treated with a further 188 ml of the methoxide solution followed by carbon disulphide (19 ml). After cooling again to 20°C, the remaining methoxide solution was added, followed by carbon disulphide (9 ml) at 20°C. The total of carbon disulphide added was 65.5 ml. When the additions were complete, the mixture was stirred at room temperature for 3 hours. Potassium iodide (3g) was added followed dropwise by benzyl chloride (275.8g). The temperature rose to about 55°C during the addition. The mixture was then boiled under reflux for 1 hour. After cooling, the mixture was poured into 5 1 of iced water, and the solid material was filtered off, washed thoroughly with water and dried, to give 320.2g of desired product.

(b) Methyl 3-amino-5-benzylthiopyrazole-4-carboxylate

0056A stirred mixture of the product of stage (a) (87.0g) and hydrazine hydrate (12.3g) in methanol (340 ml) was boiled under reflux for 5 hours. After cooling, the mixture was evaporated to dryness. The residue was stirred with sodium carbonate solution for 15 minutes. The solid was filtered off, washed with water and recrystallised from acetonitrile to give 28.9g of desired product, mp 103-106°C.

(c) Methyl 2-benzylthio-5,7-dimethylpyrazolo[1,5-a] -pyrimidine-3-carboxylate

0057A stirred mixture of the product of stage (b) (54.7g) and pentane-2,4-dione (20.8g) in glacial acetic acid (425 ml) was boiled under reflux for 3 hours. After standing at room temperature overnight, the solid was filtered off and recrystallised from acetonitrile to give 54.4g of desired product, mp 161-164°C.

Examples A2-A28

0058The following compounds of formula I where R⁶ is hydrogen, and X represents -SCH₂- were prepared by methods analogous to that of Example A, but using appropriately protected ketones of formula III where necessary: <tables id="tabl0001" num="0001"><img file="EP0244097A2_D0007.tif" /></tables><tables id="tabl0002" num="0002"><img file="EP0244097A2_D0008.tif" /></tables>

0059The following compounds of formula _I where _R⁴ represents phenyl and X represents -SCH₂- were also prepared by methods analogous to that of Example A: <tables id="tabl0003" num="0003"><img file="EP0244097A2_D0009.tif" /></tables>

Preparative Example B

2-Chlorobenzylsulphinyl)-5,7-dimethylpyrazolo[1.5-a] -pyrimidine-3-carbonitrile (Compound B1)

0060A stirred mixture of the product of Example A3 (20g), dichloromethane (270ml), water (140ml) and concentrated hydrochloric acid (17.7ml) was cooled to about -3°C and treated dropwise with a solution of sodium hypochlorite (₁4₄ ml, 11-12% available chlorine) in water (144 ml) over 20 minutes, keeping the temperature below 0°C. After stirring at 0-5°C for 45 minutes, the mixture was allowed to return to room temperature, and the layers were separated. The aqueous phase was extracted with more dichloromethane. The organic portions were combined , washed with aqueous sodium metabisulphite solution and dried over magnesium sulphate. Filtration and evaporation gave a solid residue which was triturated with hexane and filtered off. The solid material was washed with hot acetone and recrystallised from acetonitrile to give the desired compound, mp 209-2l1°C.

Preparative Example C

Methyl 2-tbenzylsulphonyl)-5,7-dimethylpyrazolo[1,5-a] -pyrimidine-3-carboxylate (Compound Cl)

0061A mixture of the product of Example A(c) (3.9g) and m-chloroperoxybenzoic acid (about 90%, 4.6g) in toluene (50ml) was boiled under reflux for 2 hours. After standing at room temperature overnight, the mixture was added to sodium carbonate solution and extracted with two portions of dichloromethane which were combined, washed with sodium carbonate solution followed by ferrous sulphate solution, and dried over magnesium sulphate. Filtration and evaporation gave a residue which was recrystallised from ethanol to give the desired compound, mp 185-188°C.

Examples C2-C3

0062The following compounds of formula I where R⁶ represents hydrogen, and X represents -S0₂CH₂- were prepared by methods analogous to that of Example C: <tables id="tabl0004" num="0004"><img file="EP0244097A2_D0010.tif" /></tables>

Preparative Example D

Methvl 2-(2,6-dichlorophenylsulphamoyl)-5,7-dimethyl -pyra2oio[1,5-a]pyrimidine-3-carboxylate (Compound D1)

(a) Methyl 2-(chlorosulphonyl)-5,7-dimethylpyrazolo[1,5-a] -pyrimidine-3-carboxylate

0063A vigorously stirred suspension of the product of Example lc (20g) in a 50/50 mixture of glacial acetic acid and water (116 ml) was cooled to below 0°C and was treated over 2 hours with chlorine whilst maintaining the temperature at 0-2°C. The solid material was then filtered off, washed with water followed by petroleum ether (60-80) and dried in vacuo to give 17.6g of desired product.

(b) Methyl 2-(2,6-dichlorophenylsulphamoyl)-5,7-dimethyl -pyrazolo[1,5-a]pyrimidine-3-carboxylate

0064A stirred solution of 2,6-dichloroaniline (10.66g) and 4-(dimethylamino)pyridine (0.4g) in dry pyridine (100 ml) was treated portionwise with the crude product of stage (a) (10.0g). A yellow precipitate appeared immediately following the addition. The suspension was stirred overnight at room temperature, and the precipitate redissolved. The mixture was then evaporated to dryness, the residue being dissolved in dichloromethane, washed with two portions of dilute hydrochloric acid, and dried over magnesium sulphate. Filtration and evaporation gave a residue which was triturated with methanol. The solid product was filtered off and recrystallised from acetonitrile to give 5.5g of the desired compound, mp 250-252°C.

Examples D2-D54

0065The following compounds of formula _I where R⁶ represents hydrogen, and X represents -SO₂NH- were prepared by methods analogous to that of Example D: <tables id="tabl0005" num="0005"><img file="EP0244097A2_D0011.tif" /></tables><tables id="tabl0006" num="0006"><img file="EP0244097A2_D0012.tif" /></tables><tables id="tabl0007" num="0007"><img file="EP0244097A2_D0013.tif" /></tables>

0066The following compound where _R⁴ represents 2-methoxycarbonyl-4-methylthien-3-yl, R⁶ represents hydrogen, and X represents -SO₂NH- was also prepared by a method analogous to that of Example D: <tables id="tabl0008" num="0008"><img file="EP0244097A2_D0014.tif" /></tables>

0067The following compounds where X represents -SO₂NH- were also prepared by methods analogous to that of Example D: <tables id="tabl0009" num="0009"><img file="EP0244097A2_D0015.tif" /></tables>

0068Preparative Example E

Z-Chloro-N-(3-cyano-5,7-dimethylpyrazolo[1,5-a]pyrimidin-2-yl)benzenesulphonamide (Compound El)

(a)N2-(I-amino-2,2-dicyanovinyl)benzohydrazide

0069A stirred mixture of potassium tricyanomethanide (69.7g), benzohydrazide (68.0g) and water (350 ml) was treated with concentrated hydrochloric acid (45 ml) and the mixture was heated to boiling. A very thick precipitate quickly formed, and the mixture was boiled gently for 5 hours. After standing overnight, the solid was filtered off, washed with water and air-dried to give 100.3g of desired product, mp 182-186°C.

(b) 3,5-Diaminopyrazole-4-carbonitrile

0070A mixture of the product of stage (a) and 1M sodium hydroxide solution (750 ml) was stirred at room temperature for 5 hours. It was then filtered and the filtrate was acidified with concentrated hydrochloric acid. The precipitated solid was filtered off and washed with water. The filtrate was washed with three portions of dichloromethane, made alkaline with potassium carbonate, and evaporated to dryness. The residue was extracted thoroughly with acetone, which was evaporated to dryness to give a yellow solid material in a yield of 20.3g, mp 139-142°C.

(c) 2-Amino-5,7-dimethylpyrazolo[1,5-a]pyrimidine-3-carbonitrile

0071A stirred mixture of the product of stage (b) (₂0.2g). 2,4-pentanedione (18.5g) and triethylamine (15 drops) in ethanol (375 ml) was boiled under reflux for ₅ hours. After cooling, the solid material was filtered off and the filtrate was evaporated to dryness. The residue was recrystallised from acetonitrile along with the filtered solid to yield 6.1g of desired product.

(d) 2-Chloro-N-(3-cyano-5,7-dimethylpyrazolo[1,5-a]-pyrimidin-2-vl)benzenesulphonamide

0072A mixture of the amine product of stage (c) (2.81g), 2-chlorobenzenesulphonyl chloride (3.17g) and a catalytic amount of 4-dimethylaminopyridine in dry pyridine (35 ml) was stirred at room temperature for two days. It was then poured into water and extracted with three portions of dichloromethane which were combined and dried over magnesium sulphate. Filtration and evaporation gave a residue which was recrystallised from acetonitrile. It was then recrystallised a second time from methanol to give 1.3g of desired product, mp 205-208°C.

Examples E2-E4

0073The following compound of formula I where R⁶ represents hydrogen was prepared by a method analogous to that of

Example E:

0074<tables id="tabl0010" num="0010"><img file="EP0244097A2_D0016.tif" /></tables>

Preparative Example F

Methyl 5,7-dimethyl-2-(2-methylphenoxysulphonyl)-pyrazolo(1,5-a)pyrimidine-3-carboxylate

0075A stirred solution of the product of Example D(a) (2.0 g) and o-cresol (0.71 g) in dry pyridine (30 ml) was heated at about 60°C for 3 hours. After standing at room temperature for 2 days, the mixture was poured into water and extracted with 3 portions of dichloromethane which were combined, washed twice with dilute hydrochloric acidand dried over magnesium sulphate. Filtration and evaporation gave a residue which was recrystallised from methanol to give 1.1 g of desired product. mp 112-114°C.

Preparative Example G

Methyl 2-(2,6-dichlorophenyl-N-propargylsulphamoyl)-5,7-dimethyl-pyrazolo[1,5-a]pyrimidine-3-carboxviate (Compound G1)

0076Sodium hydride (0.3g) was added to a solution of the product of Example D (Compound Dl) (4.3g) in dimethylformamide (50 ml), and the mixture was stirred at room temperature for 10 minutes. Propargyl bromide (I.lml) was then added, and the mixture was refluxed for 5% hours, then left to cool. It was then poured into 750ml of distilled water, and the precipitate which formed was filtered off and dried. On recrystallisation from ethyl methyl ketone and then from acetonitrile. 1.3g of desired product, mp 225-229°C, was obtained.

Examples G2-G3

0077The N-allyl derivative (Compound G2), mp 157-160°C, and the N-methyl derivative (Compound G3), mp 202-204°C, corresponding to that of Example G were prepared by methods analogous to those described therein.

Preparative Example H

2-(2,6-Dichlorophenylsulphamoyl)-5,7-dimethylpyrazolo-[1,5-a]pyrimidine-3-carboxamide (Compound Hl)

0078The product of Example D9 (4.6g) was treated with concentrated sulphuric acid and was stirred at room temperature overnight. It was then poured into ice-water, and the precipitated solid was filtered off, washed with water and dried. It was then purified by dissolving it in dimethylformamide, filtering whilst hot, and reprecipitating the product from the filtrate by the addition of water, to give 3.0g of desired product, mp>320°C.

Examples H2-H7

0079The following compounds of formula _I where _R⁶ represents hydrogen were prepared by methods analogous to that of Example H: <tables id="tabl0011" num="0011"><img file="EP0244097A2_D0017.tif" /></tables>

0080The following compound of formula _I where R and R⁶ together represent -(CH₂)₃- was prepared by methods analogous to that of Example H: <tables id="tabl0012" num="0012"><img file="EP0244097A2_D0018.tif" /></tables>

Preparative Example I

2-(2,6-Dichlorophenylsulphamovl)-N,5,7-trimethylpyrazolo-[1,5-a]pyrimidine-3-carboxamide (Compound Il)

0081A suspension of the product of Example Dl (4g) in 33% methylamine solution in industrial methylated spirits (65 ml) was heated in a small autoclave at about 100°C overnight. After cooling, the resultant mixture was filtered, the filtered solid being washed with methanol, dried and recrystallised from glacial acetic acid, to give 1.6g of desired product, mp 284-286°C.

Example 12

0082The following compound of formula _I where R⁶ represents hydrogen was prepared by a method analogous to that of Example I: <tables id="tabl0013" num="0013"><img file="EP0244097A2_D0019.tif" /></tables>

Preparative Example J

2-Ethoxyethyl 2-(2,6-dichlorophenylsulphamoyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidine-3-carboxvlate

0083A suspension of the product of Example Dl (1.0g) in 2-ethoxyethanol (25ml) was treated with titanium(IV)-butoxide (0.34g) and was refluxed overnight. After cooling, the mixture was poured into dilute hydrochloric acid and extracted with three portions of dichloromethane. These were combined, washed with sodium hydrogen carbonate solution and dried over magnesium sulphate. Filtration and evaporation gave a residue to which toluene was added. The solid material was filtered off, washed with toluene and dried to give 0.8g of desired product, mp 198-20p°C.

Preparative Example K

2-(2,6-Dichlorophenylsulphamoyl)-5,7-dimethylpyrazolo-[1,5-alpyrimidine-3-carbohydrazide

0084Hydrazine hydrate (0.56ml) was added to a suspension of the product of Example Dl in ethanol (50ml). The mixture was refluxed for 5 days, left to cool to room temperature, and then evaporated to give 4.0g of desired product as a yellow solid, m,p 285-287°C.

Preparative Example L

Isopropyl 2-(2.6-dichlorophenylsulphamoyl)-5,7-dimethyl -pyrazolo[1.5-a]pyrimidine-3-carboxylate

0085The product of Example D7 (0.8g) in trifluoroacetic anhydride (4ml) was treated with trifluoroacetic acid (2ml), and the resultant solution was treated carefully with propan-2-ol (20ml) and refluxed for 1 hour. The mixture was then evaporated to dryness, the residue was mixed with water, and the solid material was filtered off and recrystallised from propan-2-ol to give 0.6g of the desired product, mp 190-192°C, identical to that of

Example D15.

Preparative Example M

Methyl 2-(2.6-dichlorophenylsulphamoyl)-5-hydroxy-7-methyl -pyrazolo[1,5-a]pyrimidine-3-carboxylate (Compound Ml)

(a) Methyl 3-acetamido-5-benzylthiopyrazole-4-carboxylate

0086The product of Example A(b) (50g), acetic anhydride (20g) and acetic acid (100g) were refluxed with stirring for 6 hours. The solvent was then removed, and the residue was recrystallised from toluene to give 49.5g of desired product.

(b) Methyl 5-acetamido-3-chlorasulphonyl-1H-pyrazole-4-carboxylate

0087The product of stage (a) (20g) was suspended in concentrated hydrochloric acid and was cooled to -5°C. Acetic acid (15ml) was then added, and chlorine gas was bubbled through with stirring and cooling to maintain the temperature between -5°C and 0°C. A further 10ml of concentrated hydrochloric acid was then added, and after 70 minutes, the solid was filtered off and washed with water/petrol (60-80) to give 15g of desired product.

(c) Methyl 5-acetamido-3-(2,6-dichlorophenylsulphamoyl)-1H-pyrazole-4-carboxylate

00882,6-dichloroaniline (5g) and N,N-dimethyl-4-aminopyridine (0.3g) were dissolved in pyridine (50ml), and the product of stage (a) (5g) was added in portions. The mixture was then stirred at room temperature for 24 hours, after which the pyridine was removed, and the mixture was taken into dichloromethane. It was then washed with water, dried, and the solvent was removed. The residue was recrystallised from acetonitrile to give 3.6g of desired product.

(d) Methyl 5-amino-3-(2,6-dichlorophenylsulphamoyl)-1H-pyrazole-4-carboxylate

0089The product of stage (c) (lOg) was dissolved in methanol (50ml) and potassium carbonate (2g) was added. The reaction mixture was then refluxed for 6 hours, allowed to cool, and was poured into 1500ml water. The mixture was acidified with hydrochloric acid to precipitate the desired product in a yield of 5.4g.

(e) Methyl 2-(2,6-dichlorophenylsulphamoyl)-5-hydroxy-7-methyl-pyrazolo[1,5-a]pyrimidine-3-carboxglate

0090The product of stage (d) (7g) was refluxed overnight with methyl acetoacetate (3g) in trifluoroacetic acid (25ml). The solvent was then removed and the residue was recrystallised from acetonitrile to give 5.6g of desired product, mp 310-312°C.

Example M2

0091The compound corresponding to that of Example M where the substituent at the 7-position is hydroxy was prepared by an analogous method, mp 264-267°C.

Preparative Example N

Methyl 2-(2,6-dichlorophenylsulphamoyl)-5-chloro-7-methyl- pyrazolo[1,5-a]pyrimidine-3-carboxylate

0092The product of Example M (4g) was dissolved in pyridine (5ml), and thionyl chloride (25ml) was added. The reaction mixture was then heated to reflux for 4 hours, the solvent was removed, and the residue was purified by chromatography to give 3.6g of the desired product. mp 256-257°C.

Formulation Example

0093A 10% suspension concentrate of compound Dl was prepared from the following ingredients: <tables id="tabl0014" num="0014"><img file="EP0244097A2_D0020.tif" /></tables>

0094Analogous suspension concentrate formulations were prepared containing 0.5, 5, 10, 25, 50 and 85% by weight of the compounds of Examples Al-A28, B, Cl-C3, Dl-D54, El-E4, F, G1-G3, Hl-H7, I1-I2, J, K and L.

HERBICIDAL EXAMPLE A (Pre-Emerqence)

0095Seeds of the weed species listed below were sown in anodised aluminium pans 19 cm long x 9.5 cm wide x 6 cm deep, containing sterilized sandy loam. They were watered and then sprayed with the compounds of the Examples listed below formulated as a solution/suspension in 1:1 by volume of acetone and the wetting agent polyoxyethylene (20 mols) monolaurate solution (2 g per litre).

0096The concentration of each test compound and volume of application were calculated to give the desired rate of application of the compound in 450 litres per hectare. After 3 to 4 weeks growth in the controlled environment room (20°C: 75-95% relative humidity; 14 hours per day artificial illumination) the plants were visually assessed for any herbicidal response.

0097All differences from an untreated control were scored accordingly to an index where 0 = no effect. 1 = 1-24% effect, 2 = 25-69% effect, 3 = 70-89% effect and 4 = 90-100% effect. In the table below, the following letters are used to denote the plant species: <tables id="tabl0015" num="0015"><img file="EP0244097A2_D0021.tif" /></tables>

0098The results obtained were as follows: <tables id="tabl0016" num="0016"><img file="EP0244097A2_D0022.tif" /></tables><tables id="tabl0017" num="0017"><img file="EP0244097A2_D0023.tif" /></tables>

HERBICIDAL EXAMPLE B (Post-Emergence)

0099Seeds of the plant species listed below were sown in anodised aluminium pans, 19 cm long x 9.5 cm x 6 cm deep, containing sterilised sandy loam. They were then watered and placed in a controlled environment room (20°C: 75-95% relative humidity; 14 hours per day artificial illumination). Fourteen or twenty one days after sowing (depending on the species but when most plants had 2 to 3 true leaves) the seedlings received a foliar spray of the compounds of the Examples listed below. formulated as a solution/suspension in 1:1 by volume of acetone and the wetting agent polyoxyethylene (20 mols) monolaurate solution (2 g per litre).

0100The concentration of each test compound was calculated to give the desired rate of application of the compound in 450 litres per hectare. After 2 to 3 weeks growth in the controlled environment room the plants were visually assessed for any herbicidal response.

0101All differences from an untreated control were scored according to an index where 0 = no effect, 1 = 1-24% effect, 2 = 25-69% effect. 3 = 70-89% effect and 4 = 90-100% effect. In the table below, the letters used denote the same plant species as in Herbicidal Example A: <ul id="ul0002" list-style="none"><li>The results obtained were as follows:<img file="EP0244097A2_D0024.tif" /><img file="EP0244097A2_D0025.tif" /></li></ul>