Accelerated nucleic acid reassociation method
Abstract
A method for the formation of double stranded nucleic acid molecules from separate single stranded nucleic acid molecules in a single phase reaction solution is disclosed wherein the rate of reaction is greatly increased over the rate of reaction at standard reference conditions. The greatly accelerated reaction rate is accomplished through the use of known concentrations of nucleic acid precipitating agents which are added to the reaction solution. Nucleic acid denaturing agents may also be added. The solution so formed is incubated and then assayed for the presence of double stranded nucleic acid molecules.
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Projected expiry passed 28 June 2005, 21.2 years ago.
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Every citation, both waysCites: the store holds 2 of 3
| Document | Relation | Office | Category | Cited during |
|---|---|---|---|---|
| EP0127327A1 | Cites | European Patent Office (EPO) | AP | Search report |
| WO8402721A1 | Cites | World Intellectual Property Organization (WIPO) | XP | Search report |
| CHEMICAL ABSTRACTS, vol. 87, no. 25, December 1977, page 331, abstract no. 196819n, Columbus, Ohio, US; D.E. KOHNE et al.: "Room temperature method for increasing the rate of DNA reassociation by many thousandfold: the phenol emulsion reassociation technique", & BIOCHEMISTRY 1977, 16(24), 5329-41 | Non-patent | – | – | Search report |
27 members in 9 offices; this record represents the family
Priority claims5
| Document | Office | Kind | Date |
|---|---|---|---|
| 627795 | United States of America | – | |
| 62779584 | United States of America | A | |
| 62779584 | United States of America | A | |
| 627795 | – | – | – |
| US19840627795 | – | – | – |
Members27
| Document | Office | Kind | |
|---|---|---|---|
| FI852625A0 | Finland | A0 | |
| IL75648A0 | Israel | A0 | |
| IL75648D0 | Israel | D0 | |
| FI852625L | Finland | L | |
| EP0167366A2 | European Patent Office (EPO) | A2 | |
| AU4457385A | Australia | A | |
| JPS6181800A | Japan | A | |
| EP0167366A3This record | European Patent Office (EPO) | A3 | |
| AU5879986A | Australia | A | |
| JPS62158500A | Japan | A | |
| EP0229442A1 | European Patent Office (EPO) | A1 | |
| AU603767B2 | Australia | B2 | |
| US5132207A | United States of America | A | |
| EP0229442B1 | European Patent Office (EPO) | B1 | |
| AT85088T | Austria | T | |
| ATE85088T1 | Austria | T1 | |
| DE3687645D1 | Germany | D1 | |
| DE3687645T2 | Germany | T2 | |
| JPH0789955B2 | Japan | B2 | |
| CA1337551C | Canada | C | |
| CA1337753C | Canada | C | |
| JPH082319B2 | Japan | B2 | |
| EP0167366B1 | European Patent Office (EPO) | B1 | |
| AT152116T | Austria | T | |
| ATE152116T1 | Austria | T1 | |
| DE3588154D1 | Germany | D1 | |
| DE3588154T2 | Germany | T2 |
43 legal events, as 3 offices reported them to INPADOC
Over the term
Point at a mark for the eventEvents
| Event | Code | Office | |
|---|---|---|---|
| Appeal reference modifiedAppealORIGINAL CODE: EPIDOSCREFNOAPAH | APAH | EP | |
| Nl: ceased due to reaching the maximum lifetime of a patentCeasedNLV7 | NLV7 | EP | |
| Se: european patent has lapsedLapsedEUG | EUG | EP | |
| Patent ceasedCeasedPL | PL | CH | |
| Patent expired after termination of 20 yearsExpiredPE20 | PE20 | GB | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| European patent in force as of 2002-01-01IF02 | IF02 | GB | |
| Be: lapsedLapsedBERE | BERE | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Lapsed in a contracting state [announced via postgrant information from national office to epo]LapsedPG25 | PG25 | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| Annual fee paid to national office [announced via postgrant information from national office to epo]GrantedPGFP | PGFP | EP | |
| No opposition filedOpposition26N | 26N | EP | |
| No opposition filed within time limitOppositionORIGINAL CODE: 0009261PLBE | PLBE | EP | |
| Information on the status of an ep patent application or granted ep patentGrantedSTATUS: NO OPPOSITION FILED WITHIN TIME LIMITSTAA | STAA | EP | |
| Fr: translation filedET | ET | EP | |
| Corresponds to:REF | REF | EP | |
| It: translation for a ep patent filedITF | ITF | EP | |
| It: translation for a ep patent filedITF | ITF | EP | |
| European patent takes effect as a national patent in ch/liEP | EP | CH | |
| New agentNV | NV | CH | |
| Designated contracting statesAK | AK | EP | |
| Corresponds to:REF | REF | EP | |
| (expected) grantORIGINAL CODE: 0009210GRAA | GRAA | EP | |
| Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOS IGRAGRAH | GRAH | EP | |
| Despatch of communication of intention to grant a patentORIGINAL CODE: EPIDOS IGRAGRAH | GRAH | EP | |
| Despatch of communication of intention to grantORIGINAL CODE: EPIDOS AGRAGRAG | GRAG | EP | |
| First examination report despatched17Q | 17Q | EP | |
| Party data changed (applicant data changed or rights of an application transferred)RAP1 | RAP1 | EP | |
| Request for examination filed17P | 17P | EP | |
| Designated contracting statesAK | AK | EP | |
| Search report despatchedORIGINAL CODE: 0009013PUAL | PUAL | EP | |
| Designated contracting statesAK | AK | EP | |
| Public reference made under article 153(3) epc to a published international application that has entered the european phaseORIGINAL CODE: 0009012PUAI | PUAI | EP |
Numbers
- Publication
- 0167366
- Publication, DOCDB
- 0167366
- Publication, EPODOC
- EP0167366
- Application
- 85304635
- Application, DOCDB
- 85304635
- Application, EPODOC
- EP19850304635
Titles3
- English
- Accelerated nucleic acid reassociation method
- French
- Méthode accélérée pour la réassociation d'acides nucléiques
- German
- Schnellere Methode für die Reassoziation von Nukleinsäure
Classification
- CPC, 2
- C07H21/00
- C12Q1/6832
- IPC, 6
- G01N33 50
- C07H21 00
- C12N15 00
- C12N15 09
- C12Q1 68
- G01N33 53
Designated states1
- Contracting states, 1
- Sweden