EP0167366A3

Accelerated nucleic acid reassociation method

Abstract

A method for the formation of double stranded nucleic acid molecules from separate single stranded nucleic acid molecules in a single phase reaction solution is disclosed wherein the rate of reaction is greatly increased over the rate of reaction at standard reference conditions. The greatly accelerated reaction rate is accomplished through the use of known concentrations of nucleic acid precipitating agents which are added to the reaction solution. Nucleic acid denaturing agents may also be added. The solution so formed is incubated and then assayed for the presence of double stranded nucleic acid molecules.

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Projected expiry passed 28 June 2005, 21.2 years ago.

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Every citation, both waysCites: the store holds 2 of 3

Citations
DocumentRelationOfficeCategoryCited during
EP0127327A1CitesEuropean Patent Office (EPO)APSearch report
WO8402721A1CitesWorld Intellectual Property Organization (WIPO)XPSearch report
CHEMICAL ABSTRACTS, vol. 87, no. 25, December 1977, page 331, abstract no. 196819n, Columbus, Ohio, US; D.E. KOHNE et al.: "Room temperature method for increasing the rate of DNA reassociation by many thousandfold: the phenol emulsion reassociation technique", & BIOCHEMISTRY 1977, 16(24), 5329-41Non-patentSearch report