Process for preparation of composite materials comprising porous, hollow particles.

0001The present invention relates to materials and more particularly to composite materials and the preparation of such composite materials.

0002According to one aspect of the present invention there is provided a composite material comprising porous, hollow particles of inorganic material carrying a selected substance.

0003It will be appreciated that in accordance with the present invention the selected substance is accommodated predominately within porous shells provided by the hollow particles. It will also be-appreciated that there may be a minor proportion of selected substance in the pores of the shells and/or on the surface of the particles.

0004The particles may conveniently be substantially spheroidal or may be substantially spherical.

0005It will be understood that the porous shell of the porous, hollow particles may be permeable such as to allow reagents and reactants to reach the selected substance and to allow elution of species from the selected substance. Preferably a selected substance is introduced or formed in prefabricated porous hollow particles; in these circumstances the porous shells must be permeable such as to allow the introduction of the selected substance or introduction of reagents for forming the selected substance.

0006Thus, the term "porous" as used in this Specification embraces "permeable" as discussed in the immediately preceding paragraph herein.

0007The selected substance may be chosen from a wide range of substances according to the substance it is desired to incorporate in the composite material. For example, the selected substance may be an active organic gel. By way of example, such a gel may be active with regard to chemical species. Thus, for example, a gel may be active with regard to chemical species by predominately chemical interaction (e.g. the gel may have ion-exchange properties, affinity chromatography properties, or ampholytic properties) or may be active with regard to chemical species by predominately physical interaction (such as in gel filtration and molecular sieving processes).

0008By way of further example the selected substance may be a biochemically active substance such as a substance consisting of or containing biological cells or an enzyme. The biologically active substance may be in the form of an organic gel.

0009By way of yet further example the selected substance may be such that the composite material can be used in two-phase separation. Thus, for example, where the composite material is to be used in aqueous media the selected substance may be an organic substance (e.g. a liquid) substantially immiscible with aqueous media.

0010It is to be further understood that in accordance with the present invention the porous hollow particles of inorganic material may be considered to be acting as a "support material" for a selected substance whenever a selected substance is carried by the porous inorganic material. This embraces, for example, where the porous inorganic material adds mechanical stability to deformable substances (e.g. as in the case of a selected substance comprising an organic deformable gel) and also embraces where the porous inorganic material carries the selected substance without necessarily providing it with mechanical stability throughout, for example as in the case when a liquid is retained within a hollow porous particle of inorganic material in accordance with the present invention.

0011According to a further aspect of the present invention there is provided a process for the preparation of a composite material comprising porous hollow particles of inorganic material carrying a selected substance, which comprises coating a fugitive core material with inorganic material or a precursor therefor, removing the fugitive core material to produce porous hollow particles of inorganic material and introducing the selected substance or a precursor therefor to the porous, hollow particles.

0012By way of example the coating of the fugitive core material may be effected by applying inorganic material powder to the fugitive core material in the presence of a small amount of water to make the powder moist and adherent. As an alternative to using a small amount of water an aqueous solution (e.g. an aqueous solution of a metal salt) may be used to moisten the powder and by this means it is possible to introduce further inorganic material to the coating.

0013The addition of inorganic material and water (or aqueous solution) during coating should be controlled so that no excess of either occurs and the coating remains uniform.

0014The coating may be conveniently carried out for example, in a spheroidiser. Thus the powder moistened as hereinbefore disclosed may be applied to the fugitive core material until a sufficiently thick coating has been built up on the core material.

0015Preferably the core material is a fugitive core material which can be decomposed on heating so that after coating core material can be removed by the application of heat.

0016Examples of fugitive core materials are organic resin beads (i.e. synthetic ion-exchange resin beads) and alginate spheres.

0017Where the fugitive core material is to be removed by heating it should be combustible and released in a controlled manner (e.g. as C0₂ finally) through the porosity of the "shell". The fugitive core material and products produced therefrom on heating should not interact adversely with the "shell". (For example, some materials (e.g. polysulphonated resins) decompose giving S0₂ which can react undesirably with some inorganic materials of the shell).

0018Subsequently to the fabrication of the porous hollow particles the selected substance can be introduced as hereinbefore discussed.

0019The hollow porous particles of inorganic material may be arranged to carry a selected material by any convenient method. Where the selected substance is a liquid the liquid may be introduced through the particles and retained therein by surface tension forces or by forming a membrane around each of the particles.

0020A precursor for a selected substance may be introduced to the particles and subsequently treated to form the selected substance. For example, a fluid precursor may be introduced to the particles and subsequently gelled (e.g. by precipitation and/or cross-linking, or by polymerisation).

0021Reference may be made to British Patent Specification No. 1514707 and British Patent Specification No. 1602432 by way of example with regard to examples of suitable methods of forming selected substances in a support material.

0022Any suitable inorganic material may be used to form porous hollow particles for use in accordance with the present invention. Examples are titanium dioxide, calcium phosphate, aluminium oxide, zirconium oxide, barium sulphate, calcium sulphate and natural earths such as bentonite, kieselguhr and the diatomaceous substance available under the Trade Name of "Celite".

0023Mixtures of inorganic materials may be used in the formation of hollow spheres for use in accordance with the present invention. (For example a powder mixture of kieselguhr with the addition of a further inorganic material such as an oxide (e.g. titania) may be used). As hereinbefore mentioned a solution containing a further inorganic material may be used to moisten powders applied to a fugitive core material in the preparation of particles suitable for use in accordance with the present invention so that it is possible to prepare porous hollow particles (e.g. spheres) of inorganic material of a desired composition.

0024According to a further aspect of the present invention there is provided a process for the selective separation of a chosen component from a fluid which comprises contacting the fluid with a composite material comprising porous hollow particles of inorganic material carrying a selected substance capable of retaining the chosen component from the fluid such that the chosen component is retained by the selected substance.

0025The chosen component (e.g. a chosen chemical species) may subsequently be recovered from the composite particles and the particles reused, if desired, in a further separation operation.

0026By way of example the composite material may comprise porous hollow particles of inorganic material containing a selected substance comprising an affinity chromatography reagent. Thus, for example, composite material comprising porous hollow particles of inorganic material (e.g. kieselguhr) containing and carrying an organic affinity chromatography gel (e.g. agarose gel carrying an affinity chromatography agent (such as the dye Cibacron Blue 3G-A ex. Ciba-Geigy)) can be contacted with a biologically produced fluid (e.g. a raw cell culture or fermentation broth) so that the gel removes the selected component into the particles from the fluid by affinity chromatography. Subsequently the selected components may be removed from the particles by suitable elution.

0027The present invention will now be described by way of example only as follows:

Example 1

0028A solution of LB Manucol (sodium alginate ex Alginate Industries Ltd.) (60g), glycerol (100 ml) and demineralised water (1 litre) was added dropwise to a solution of calcium chloride (30g), glycerol (300 ml) and water (3 litres) to produce precipitated alginate spheres.

0029These spheres were dried at room temperature overnight such that they remained moist and their final size of 3 to 4 millimetres diameter. The moist spheres were spheroidised with incremental amounts of kieselguhr and water to produce coatings of approximately 0.5 millimetre thickness on the alginate spheres.

0030The coated alginate spheres were sintered at 600°C for 24 hours and at 850°C for one hour to give robust, symmetrical, porous, hollow spheres of ~4 millimetres outside diameter.

Example 2

0031The procedure of Example 1 was followed with the exception that the alginate spheres were dried at room temperature over a period of several days to a size of 1 to 2 millimetres diameter. These spheres were then sprayed with water and spheroidised with incremental amounts of kieselguhr and water to build up spheres with an approximate coating of .5 millimetre thickness. The coated spheres were sintered at 600°C for 24 hours and at 850°C for one hour. This gave robust, symmetrical, porous, hollow spheres of approximately 3 millimetres diameter.

Example 3

0032A solution containing LM Manucol (sodium alginate ex Alginate Industries Ltd) (5g), glycerol (10m) and demineralised water (1 litre) was added dropwise to a solution of calcium chloride (30g), glycerol (30ml) and water (3 litres) to produce alginate spheres. These were carefully dried first with methanol and then in an oven at 70°C to give dried spheres of -400 microns diameter.

0033These spheres were grown to 800 micron spheres in a spheroidiser with incremental additions of kieselguhr and water. Finally the resulting coated spheres were sintered at 850°C for one hour to give porous, hollow spheres.

Example 4

0034Amberlyst A-26 (macro-reticular resin beads ex BDH) was sieved and a 400 micron fraction was coated with kieselguhr and water by incremental additions in a spheroidiser as in Example 3 to give spheroids of 500 to 1000 microns outside diameter.

0035The spheroids were sintered at 800°C for one hour. This gave porous, hollow spheres.

Example 5

0036Amberlyst A-26 (600 micron diameter fraction), (50g), was well moistened and coated in a spheroidiser with incremental additions of an intimate mixture of titania (ex May and Baker) (80g) and Amberlyst A-26 (ground and sieved to <80 microns) (10g) with water as a binder. The resulting spheres (which were ~800 microns in diameter) were sintered at 900°C for one hour to give porous hollow spheres of titania.

Example 6

0037Porous, hollow spheres of kieselguhr (700 - 800µ diameter) were prepared in a manner similar to that of

Example 3.

0038A sample (25 ml) of these spheres was prewarmed (50°C), impregnated with molten agarose (4% w/v in water) and autoclaved at 10 psi for 20 minutes.

0039When cool the resulting material was rubbed through a 1000µ mesh sieve. The excess, free and less dense agarose was separated and removed by decantation to leave composite material spheres comprising hollow kieselguhr particles containing agarose gel.

Example 7

0040Composite spheres prepared as in Example 6 were drained and impregnated with 4% Cibacron Blue 3 GA dye (Ciba-Geigy) on a water bath at 70°C; the spheres and dye were equilibrated at this temperature before mixing together.

0041After 2 hours solid NaCl (4.5g) followed by Na₂CO₃ (8.1 ml of 10% w/v) was added to the mixture of spheres and dye and mixed well. The resulting mixture was heated at 70°C for 17 hours after which it was allowed to cool. Excess dye was washed off by decantation and the resulting spheres drained and stirred in a 0.1% w/v solution of sodium azide.

0042The resulting product comprised composite spheres with each composite sphere comprising hollow kieselguhr particles containing an agarose gel carrying Cibacron Blue 3 GA dye affinity chromatography agent.

0043Sample composite spheres were sectioned and showed substantially complete filling of the interior of the hollow spheres with agarose gel and a substantially uniform uptake of affinity chromatography agent dye by the agarose gel.

Example 8

0044A 10ml column of composite material spheres prepared in accordance with Example 7 was prepared and first washed with 1 : 1 w/v ethanol/water for 4 hours to remove labile dye and any other non-firmly bound material then with 1% w/v NaCl until a steady base line was shown on a recorder coupled to a u.v, monitor absorbing at 280 nm.

0045The column was loaded with 1.2% w/v Human Serum Albumin (HSA) in 1% NaCl to an equilibrated plateau, washed with 1% w/v NaCl to remove unbound H.S.A. and then eluted with 2% KSCN in 1% NaCl. Fractions were pooled for analysis.

0046Results are given in the Tables 1 and 2.

Example 9

0047The procedure of Example 8 was repeated with another sample of composite material spheres.

0048Results are given in the Tables 1 and 2. <tables id="tabl0001" num="0001"><img file="EP0154751A2_D0001.tif" /></tables><tables id="tabl0002" num="0002"><img file="EP0154751A2_D0002.tif" /></tables>