Mouse lymphocytar hybridom vu ifn al 6

(57) The invention relates to a murine lymphocyte hybridoma producing an antibody against human leukocyte acid-labile interferon alpha deposited in the collection of hybridomas of the Virology Institute of SAS in Bratislava under the designation IF IFN AL6. The IFN AL6 hybridoma monoclonal antibody is suitable for determining the presence of acid-labile interferon alpha by inhibiting its biological activity in interferon neutralization assays. The monoclonal antibody is also suitable for the preparation of sera specific for murine immunoglobulin of subclass IgG2a. CS 267392 B1 CS267 392B1 1

The invention relates to a novel hybridoma, i.e., a hybrid single-stranded organism, a buildup of mouse myeloma NSO cell line and a mouse slice lymphoid cell that produces an anti-human acid antibody (ankylosing intravassion,

Additionally, antibodies to interferon are made by interferon injection repeatedly as an antigen in experimental animals, most commonly rabbits or sheep. The serum of such immunized animals, taken after a certain antigenic antigen time, serves as a source of antibodies used primarily for quantitative or qualitative analysis of the antigen - the interferon molecule - in research and immunodiagnostic practice. This procedure, called conventional immunization, has several disadvantages. In the sera of immunized animals there is a heterogeneous mixture of antibodies, the spectrum of which in each individual organism is unusual and unrepeatable. Generally, the organism will create, in addition to antibodies against the desired antigen, antibodies to impurities of the antigenic preparation that are required to remove from the serum by shedding. Thus, conventional batch serum production is difficult to standardize and is within a wide range of quality. For the production of each batch, a clean immunization antigen and other antigens should be prepared to remove ballast antibodies against impurities.

The aforementioned drawbacks of the above-mentioned and commonly used procedure are eliminated when a hybridoma producing a monoclonal antibody against the human acid-labile interferon alpha is deposited in the collection of hybridomas of the SIA Virology Institute in Bratislava, Mlýnská dolina 1, under the name of the IFN AL6.

Said hybridoma was obtained by a method known from the literature (Kohler, G., Milstein, C., Continuous Cultures of Fused Cell Secreting Antibodies of predefined specificity, Nature, 256, 1975), 495; Gerhard, W .: Fusion of cells in suspension and outgrowth of hybrids in conditioned media. In: Monoclonal antibodies. Hybrids: A New Dimension in Biological Analyzes. RH Kennet, TJ McKearn, B. Bechtol, eds., New York, Plenum Press, (1980), 370) by cloning a set of hybrid cells after mouse myeloid lineage of NSO and cells derived from spleen of BALB / c mouse mice immunized with a preparation of human leukocyte acid-labile interferon alpha . The advantage of the hybridoma is that it produces homo-